CN109430529A - A kind of preparation method of novel glycolysis injection bean feed - Google Patents
A kind of preparation method of novel glycolysis injection bean feed Download PDFInfo
- Publication number
- CN109430529A CN109430529A CN201811384755.0A CN201811384755A CN109430529A CN 109430529 A CN109430529 A CN 109430529A CN 201811384755 A CN201811384755 A CN 201811384755A CN 109430529 A CN109430529 A CN 109430529A
- Authority
- CN
- China
- Prior art keywords
- soybean
- curing
- injection
- glycolysis
- novel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of preparation method of novel glycolysis injection bean feed, includes the following steps: to crush soybean plus clean water stirring is sized mixing, big soya-bean milk is made；Soybean stock pump is entered Jet liquefier to cure, then liquid pump will be cured and enter to keep the temperature after filling is kept the temperature after cooling, addition complex enzyme zymohydrolysis, the obtained enzymatic hydrolysis of soybean that cures is starched；Curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, curing enzymatic hydrolysis of soybean thin pulp and soybean slag wet basis is made；Curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation, curing enzymatic hydrolysis of soybean thickened pulp is made；Soybean slag wet basis, dregs of beans and mixed bacteria liquid are mixed into post-fermentation, the fermentation residue dregs of rice are made；It is dry after curing enzymatic hydrolysis of soybean thickened pulp and the fermentation residue dregs of rice are mixed, novel glycolysis injection bean feed is made.Bean feed prepared by the present invention is rich in soybean protein, soyabean oligosaccharides, small peptide, lactic acid and probiotics, has the characteristics that low antigen, amino acid balance, nutrition is high, easy to digest, palatability is good and anti-nutritional factors content is low.
Description
Technical field
The invention belongs to feed, enzyme preparation and microorganisms technical fields, and in particular to a kind of novel glycolysis injection soybean feeding
The preparation method of material.
Background technique
China is populous nation and the first in the world pig raising big country.2017, the Animal Husbandry in China output value was up to 3.6 trillion
Member, live pig deliver 6.89 hundred million for sale, wherein sow livestock on hand forty-two million head, and 54,870,000 tons of pork yield.According to Ministry of Agriculture's statistical number
According to national commercial feed yield reaches 2.05 hundred million tons within 2017.Wherein, 1.79 hundred million tons of complete feed, concentrated feed 21,510,000
Ton, 6,900,000 tons of premix, including 89,740,000 tons of pig feed yield, 90,000,000 tons of fowl material yield, 53,000,000 tons of aquatic feeds, cattle and sheep
8,500,000 tons of feed, 3,500,000 tons of feeds of rabbit racoon dog pet etc., more than 600 ten thousand tons of national pig starter feed.Expect the cooperation of the year two thousand twenty whole nation
Forage yield is up to 2.2 hundred million tons or more, and wherein pig starter feed is up to more than 950 ten thousand tons or more.
It is well known that the difficult point of exploitation, the development of feed product is young age animal and fowl fodder, young age livestock and poultry how are improved
Feed digestibility and reduction reduce anti-nutritional factors as the project that Animal nutrition scholar actively inquires into recent years.With regard to young age
For livestock and poultry especially suckling piglet, metabolism is vigorous, and relative growth is rapid, and the requirement to diet nutrient substance is high.Cause
This, the feed for piglet wants compound two big conditions: first is that should nutrition is high, palatability is good and likes eating, be easy digestion suction again
It receives；Second is that neither will lead to suckling piglet indigestion and trophism diarrhea, growth and development is influenced, biological safety is very again
Height will not infect disease.Currently, the animal derived protein matter feed for meeting above-mentioned two big conditions mainly have fish meal, intestines touch albumen,
Plasma protein, whey powder, milk powder and egg powder etc., the plant source feed for meeting above-mentioned two big conditions mainly have soybean, rice
Albumen powder, wheat gluten etc..
Plasma protein refers to that the protein part in blood plasma, plasma protein are the general names of multiple proteins, and plasma protein can divide
For several ingredients such as albumin, globulin and fibrinogen.Plasma protein can effectively prevent the diseases such as grice diarrhoea, diarrhea,
Alleviate stress, promote growth, promote piglet feeding.
Dried porcine saluble is using chitterlings as single source, at a series of hydrolysis for a long time of protease and special enzymatic hydrolysis
Reason, and after the novel animal protein raw material that is manufactured through processes dry etc. under high-temperature sterilization and specific condition.Intestine membrane protein
Powder can partly or entirely replace the animal protein raw material costly such as plasma protein powder, whey powder and skimmed milk power, save significantly
About feed cost.
Soybean is important phytoprotein and oil source, has high nutritive value, obtains in animal and fowl fodder
It is widely applied.The nutrition feature of full-fat bean has: high thermal energy, high protein, high digestibility, vitamin E and ovum rich in
Phosphatide.The general ingredient of soybean are as follows: moisture≤12%, crude fat: 17-19%, thick protein: 35-39%, crude fibre:
5.0-6.0%, cinder powder: 5.0-6.0%, calcium 0.24%, phosphorus 0.58%.But the anti-nutritional factors in soybean limits greatly
Beans and its product utilize level in animal and fowl fodder.It is reacted according to feed nutritive value and animal biology, it will be in soybean
Anti-nutritional factors is divided into following six class: (1) factor of protein digestibility and utilization rate is reduced, such as protease inhibitor, gruel
Protease inhibitor, agglutinin etc.；(2) the carbohydrate digestion rate factor is reduced, such as phenolic compound such as tannin and widow
Sugar etc.；(3) factor of minerals utilization rate, such as phytic acid are influenced；(4) it influences vitamin activity or increases animal vitamin requirement
The factors such as the factor of amount, including antivitamin A, vitamin D, vitamin E and vitamin B12；(5) stimulate immune system because
Son and some other anti-nutritional factors, such as antigen protein, goitrogen, saponin, isoflavones and raw cyanogen glucoside；
(6) with the factor of detoxifying function, such as agglutinin in feed.Therefore, anti-nutritional factors is to influence soybean protein source to exist in soybean
Principal element used in feed, usage amount of the Yao Tigao soybean protein source in feed, it is necessary to suitable measure be taken to carry out
Processing makes Soybean Anti-nutritional Factors inactivation, passivation.Currently, world wide in reduce or eliminate the anti-nutrition of soybean protein because
The research of subproblem is constantly improve, and generallys use the methods of physics, chemistry and biology and is passivated processing.Wherein, at heat
Reason is to eliminate one of the most effectual way of Soybean Anti-nutritional Factors.
In fact, from 1917, Osborne and Mende l report boiling soybean can improve the growth performance of mouse with
Come, people have conducted extensive research the thermal stability of the soybean nutritional factor, the results showed that trypsin ihhibitor, rotten egg
White enzyme inhibition factor, agglutinin, urase, goitrogen and the antivitamin factor have to thermo-responsive characteristic, and soap
Glucoside, isoflavones, oligosaccharides, causes allergic reaction albumen and phytic acid etc. to hot more stable (Li Defa, 2003) at tannin.So heat treatment
Technology has good passivation effect to the thermal sensitivity anti-nutritional factors such as protease inhibitor, agglutinin, urase, and at present
The passivating technique that research is goed deep into the most, is most widely used.Heat treatment be broadly divided into wet heating and dry heating method (Zheng Aijuan etc.,
2002)。
Research shows that: when soybean is heat-treated, it is necessary to assure the intensity of heat treatment is suitable.Underheat then anti-nutritional factors
It destroys inadequate；Then rate of ultilization of amino acid declines for over-heating, can reduce the biological efficiency of protein.In actual production mostly with
Measurement urease activity judges the degree of passivation of the tryptose factor, reacts underheat；It is used as using albumen solubility and judges soybean
Or the index of dregs of beans over-heating.
Since upper century the nineties, cereal starch liquefaction gradually starts using injection liquefaction technology.Compared to steaming
It boils for liquefaction technology, injection liquefaction has many advantages, such as that low energy consumption, liquefaction is thorough, the more preferable and suitable large-scale production of effect.Liquid
Changing injector is the liquefied key equipment of injection, and structure is mainly by liquor inlet, steam inlet, gas-liquid mixed room, diffuser
It is constituted with separator tube.When operation, first the starch slurry of preheating is pumped by spraying system top heat to 90-95 DEG C, then with pump with steam
Liquefy injector.The saturated vapor of pressure up to 0.4-0.6MPa are continuously injected into starch slurry thin layer, spraying machinery shearing at this time is made
With starch liquefacation can be promoted.After liquefied fermented glutinous rice enters liquefied pot, 45 minutes are kept the temperature at 85-90 DEG C and completes liquefaction process.But
It is that can cereal starch injection liquefaction technology and equipment be applied to the curing of bean products and removal anti-nutritional factors still must not
And know, it is badly in need of further inquiring into research.
Feed fermentation is to convert microorganism for feedstuff for biological feed fermentation agent strain with microorganism, complex enzyme
Mycoprotein, bioactive micro peptide amino acid, microbial activity probiotics, complex enzyme formulation are integrated biological fermentation feed.
Microorganism can produce hydrolase, fermenting enzyme and respiratory enzyme during the fermentation, can eliminate the anti-nutrition in plant protein material
Substance is conducive to the digestion and absorption of animal.Fermentation process can significantly improve the palatability of soybean, there is certain phagostimulating effect.It adopts
With unique strain and zymotechnique, the protease secreted in fermentation process makes soybean protein be broken down into small molecule
Albumen and small peptide molecule.In biological fermentation process, microorganism is largely proliferated, and result not only increases fermentation soybean albumen base
The protein level of material, and it is converted into mycoprotein when the fermentation of part soybean protein, this itself also changes soybean protein
The nutritional quality of matter.
Egg also known as ovum gallinaceum, chicken are the produced ovum of hen.There is one layer of hard shell outside it, inside then there is gas chamber, albumen and ovum
Yellow part.Full of nutrition rich in cholesterol, an egg weighs about 50 grams, 6-7 containing protein grams, fatty 5-6 grams.Chicken Albumin
The amino acid ratio of matter is well suited for Human Physiology needs, is easily body absorption, and utilization rate is up to 98% or more, and nutritive value is very
Height is one of the food of mankind's often feeding.
Summary of the invention
The present invention is directed to overcome the biological insecurity of above-mentioned plasma protein and intestine membrane protein, Soybean Anti-nutritional Factors
Bad trophism, the function of young age livestock and poultry alimentary tract are unsound and its feed protein indigestion easily causes diarrhea etc.
Problem provides one kind rich in soybean protein, soyabean oligosaccharides, small peptide, lactic acid and probiotics, and with antigen, low, amino acid is put down
Weighing apparatus, the novel glycolysis for the features such as nutrition is high, easy to digest, palatability is good and anti-nutritional factors content is low injection bean feed.
The technical scheme adopted by the invention is that:
A kind of preparation method of novel glycolysis injection bean feed, includes the following steps:
1), soybean is crushed plus clean water stirring is sized mixing, big soya-bean milk is made；
2) soybean stock pump, is entered Jet liquefier to cure, then liquid pump will be cured and enter addition after heat preservation filling is kept the temperature after cooling
Curing enzymatic hydrolysis of soybean slurry is made in complex enzyme zymohydrolysis；
3), curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, curing enzymatic hydrolysis of soybean thin pulp and soybean slag wet basis is made；
4), curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation, curing enzymatic hydrolysis of soybean thickened pulp is made；
5) soybean slag wet basis, dregs of beans and mixed bacteria liquid, are mixed into post-fermentation, the fermentation residue dregs of rice are made；
6) it is mixed after, being separately dried curing enzymatic hydrolysis of soybean thickened pulp and the fermentation residue dregs of rice, or drying after mixing, is made new
Bean feed is sprayed in type glycolysis.
Preferably, in step 1), the weight ratio of soybean and clean water is 100:100~2000, and soybean smashing fineness is 8-
160 mesh.
Further, in step 1), the weight ratio of soybean and clean water is 100:400~600, and soybean smashing fineness is 50-
60 mesh.
Preferably, in step 2), injection curing temperature is 50-180 DEG C, and injection curing pressure is 0.02-6.0 MPa；
Injection curing liquid holding temperature is 30-150 DEG C, and injection curing liquid soaking time is 3-300 minutes.
Compound-enzyme feed-stuff additive amount is 0.02~200U/g substrate butt, and hydrolysis temperature is 5~80 DEG C, and enzymolysis time is
0.05~120h；
The vigor ratio of pectase, zytase, dextranase and mannase in complex enzyme be 100:0.05~
500:0.05~500:0.05~500.
Further, in step 2), injection curing temperature is 100-120 DEG C, and injection curing pressure is 0.4-0.6 MPa；
Injection curing liquid holding temperature is 70-90 DEG C, and injection curing liquid soaking time is 20-40 minutes；
Compound-enzyme feed-stuff additive amount be 4~8U/g substrate butt, hydrolysis temperature be 50~70 DEG C, enzymolysis time be 0.5~
2h；
The vigor ratio of pectase, zytase, dextranase and mannase in complex enzyme is 100:60~80:
40~60:40~60.
Preferably, in step 4), being concentrated by evaporation temperature is 40-80 DEG C, and soymilk concentrate water content is 40-80%.
Further, in step 4), being concentrated by evaporation temperature is 50-70 DEG C, and soymilk concentrate water content is 55-65%.
Preferably, in step 5), the weight ratio of soybean slag wet basis, dregs of beans and mixed bacteria liquid is 100:10~1000:
0.5~100, fermentation temperature is 5~50 DEG C, and fermentation time is 2~240h；
Lactic acid bacteria culturers number in mixed bacteria liquid: Bacillus subtilis strain number: the quantity ratio of yeast seeds number is
100:0~500:0~500.
Further, in step 5), the weight ratio of soybean slag wet basis, dregs of beans and mixed bacteria liquid be 100:50~80:5~
20, fermentation temperature is 28~30 DEG C, and fermentation time is 48~60h,
Lactic acid bacteria culturers number in mixed bacteria liquid: Bacillus subtilis strain number: the quantity ratio of yeast seeds number is
100:20~30:5~10.
Compared with prior art, the invention has the following advantages:
Bean feed is sprayed in novel glycolysis prepared by the present invention, rich in soybean protein, soyabean oligosaccharides, small peptide, lactic acid and
Probiotics has the characteristics that low antigen, amino acid balance, nutrition is high, easy to digest, palatability is good and anti-nutritional factors content is low.
Present invention discover that: cereal starch injection liquefaction technology and equipment be fully able to applied to bean products curing and
Remove anti-nutritional factors.Its technical solution: soybean being crushed after sizing mixing, and is pumped into Jet liquefier curing, after cooling down after heat preservation,
Complex enzyme zymohydrolysis is added, curing enzymatic hydrolysis of soybean slurry is made；Curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, curing enzymatic hydrolysis of soybean is made
Thin pulp and soybean slag wet basis；Curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation, curing enzymatic hydrolysis of soybean thickened pulp is made；By soybean slag
Wet basis, dregs of beans and mixed bacteria liquid mix post-fermentation, and the fermentation residue dregs of rice are made；It will curing enzymatic hydrolysis of soybean thickened pulp and the fermentation residue dregs of rice
It is mixed after being separately dried, or drying after mixing, low antigen fermentation injection beans egg feed is made.
Specific embodiment
Below with reference to embodiment, the present invention is described in detail.
Embodiment 1
After 600kg soybean impurity elimination is crushed to 50 mesh, 3000kg clean water is added, is modulated into the big soya-bean milk of 3600kg.It will
3600kg soybean stock pump enters Jet liquefier, moment injection curing under the conditions of temperature is 105 DEG C and pressure is 0.5MPa, so
Enter afterwards and retain heat preservation filling, keeps the temperature 30 minutes under the conditions of temperature is 70-90 DEG C.
When being cooled to 65-70 DEG C after heat preservation, 3.0 × 10 are added according to 5U/g substrate butt additive capacity6U is compound
3600kg curing enzymatic hydrolysis of soybean slurry is made secondary enzymolysis 1 hour under the conditions of 65 DEG C of temperature in enzyme.Wherein, the fruit in complex enzyme
Glue enzyme, zytase, dextranase and mannase vigor ratio be 100:80:40:40.
3600kg curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, 2700kg curing enzymatic hydrolysis of soybean thin pulp is made and 1000kg is big
Bean dregs wet basis.2600kg curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation under the conditions of temperature is 70 DEG C, 200kg water content is made
For 60% curing enzymatic hydrolysis of soybean thickened pulp.
After 1000kg soybean slag wet basis, 500kg dregs of beans and 60kg mixed bacteria liquid are mixed, in 28~30 DEG C of items of temperature
The 1545kg fermentation residue dregs of rice are made in secondary fermentation 48 hours under part.Wherein, the lactic acid bacteria culturers number in mixed bacteria liquid: withered grass bud
Spore bacillus species number: the quantity ratio of yeast seeds number is 100:20:5.
It is dry after 200kg curing enzymatic hydrolysis of soybean thickened pulp and the 1545kg fermentation residue dregs of rice are mixed, the low antigen of 1095kg is made
Fermentation injection beans egg feed.
Embodiment 2
After 60kg soybean impurity elimination is crushed to 60 mesh, 250kg clean water is added, is modulated into the big soya-bean milk of 310kg.It will
310kg soybean stock pump enters Jet liquefier, moment injection curing under the conditions of temperature is 110 DEG C and pressure is 0.6MPa, then
It is filled into heat preservation is retained, keeps the temperature 40 minutes under the conditions of temperature is 80 DEG C.
When being cooled to 65 DEG C after heat preservation, 3.6 × 10 are added according to 6U/g substrate butt additive capacity5U complex enzyme,
Secondary enzymolysis 1 hour under the conditions of 65 DEG C of temperature, 310kg curing enzymatic hydrolysis of soybean slurry is made.Wherein, the pectase in complex enzyme,
The vigor ratio of zytase, dextranase and mannase is 100:80:50:50.
310kg curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, 190kg curing enzymatic hydrolysis of soybean thin pulp and 120kg soybean slag is made
Wet basis.190kg curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation under the conditions of temperature is 65 DEG C, it is 60% that 20kg water content, which is made,
Curing enzymatic hydrolysis of soybean thickened pulp.
After 120kg soybean slag wet basis, 30kg dregs of beans and 5kg mixed bacteria liquid are mixed, under the conditions of 28~30 DEG C of temperature
Secondary fermentation 60 hours, the 149kg fermentation residue dregs of rice are made.Wherein, the lactic acid bacteria culturers number in mixed bacteria liquid: bacillus subtilis
Bacterium strain number: the quantity ratio of yeast seeds number is 100:30:10.
By 20kg curing enzymatic hydrolysis of soybean thickened pulp spray drying, by 149kg fermentation residue dregs of rice roller drying, the two dried object
The low antigen fermentation injection beans egg feed of 85kg is made after mixing.
Embodiment 3
After 100kg soybean impurity elimination is crushed to 30 mesh, 500kg clean water is added, is modulated into the big soya-bean milk of 600kg.It will
600kg soybean stock pump enters Jet liquefier, moment injection curing under the conditions of temperature is 120 DEG C and pressure is 0.6MPa, then
It is filled into heat preservation is retained, keeps the temperature 20 minutes under the conditions of temperature is 80 DEG C.
When being cooled to 75 DEG C after heat preservation, 6 × 10 are added according to 3U/g substrate butt additive capacity5U complex enzyme,
600kg curing enzymatic hydrolysis of soybean slurry is made in secondary enzymolysis 2 hours under the conditions of temperature 70 C.Wherein, the pectase in complex enzyme, wood
The vigor ratio of dextranase, dextranase and mannase is 100:50:20:20.
600kg curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, 380kg curing enzymatic hydrolysis of soybean thin pulp and 220kg soybean slag is made
Wet basis.380kg curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation under the conditions of temperature is 60 DEG C, it is 55% that 35kg water content, which is made,
Curing enzymatic hydrolysis of soybean thickened pulp.
After 220kg soybean slag wet basis, 50kg dregs of beans and 30kg mixed bacteria liquid are mixed, in 28~30 DEG C of conditions of temperature
The 278kg fermentation residue dregs of rice are made in lower secondary fermentation 36 hours.Wherein, the lactic acid bacteria culturers number in mixed bacteria liquid: withered grass gemma
Bacillus species number: the quantity ratio of yeast seeds number is 100:50:10.
It is dry after 35kg curing enzymatic hydrolysis of soybean thickened pulp and the 278kg fermentation residue dregs of rice are mixed, the low antigen hair of 145kg is made
Ferment sprays beans egg feed.
Embodiment 4
After 200kg soybean impurity elimination is crushed to 40 mesh, 1000kg clean water is added, is modulated into the big soya-bean milk of 1200kg.It will
1200kg soybean stock pump enters Jet liquefier, moment injection curing under the conditions of temperature is 120 DEG C and pressure is 0.6MPa, so
Enter afterwards and retain heat preservation filling, keeps the temperature 30 minutes under the conditions of temperature is 80 DEG C.
When being cooled to 70 DEG C after heat preservation, 4.0 × 10 are added according to 2U/g substrate butt additive capacity5U complex enzyme,
1200kg curing enzymatic hydrolysis of soybean slurry is made in secondary enzymolysis 2 hours under the conditions of temperature 50 C.Wherein, the pectin in complex enzyme
Enzyme, zytase, dextranase and mannase vigor ratio be 100:80:60:60.
1200kg curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, 680kg curing enzymatic hydrolysis of soybean thin pulp and 520kg soybean is made
Slag wet basis.680kg curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation under the conditions of temperature is 70 DEG C, 70kg water content, which is made, is
65% curing enzymatic hydrolysis of soybean thickened pulp.
After 520kg soybean slag wet basis, 60kg dregs of beans and 25kg mixed bacteria liquid are mixed, in 28~30 DEG C of conditions of temperature
The 598kg fermentation residue dregs of rice are made in lower secondary fermentation 36 hours.Wherein, the lactic acid bacteria culturers number in mixed bacteria liquid: withered grass gemma
Bacillus species number: the quantity ratio of yeast seeds number is 100:20:5.
By 70kg curing enzymatic hydrolysis of soybean thickened pulp spray drying, by 598kg fermentation residue dregs of rice roller drying, the two dried object
The low antigen fermentation injection beans egg feed of 255kg is made after mixing
Embodiment 5
After 300kg soybean impurity elimination is crushed to 50 mesh, 900kg clean water is added, is modulated into the big soya-bean milk of 1200kg.It will
1200kg soybean stock pump enters Jet liquefier, moment injection curing under the conditions of temperature is 120 DEG C and pressure is 0.6MPa, so
Enter afterwards and retain heat preservation filling, keeps the temperature 40 minutes under the conditions of temperature is 80 DEG C.
When being cooled to 70 DEG C after heat preservation, 3.6 × 10 are added according to 6U/g substrate butt additive capacity5U complex enzyme,
1200kg curing enzymatic hydrolysis of soybean slurry is made in secondary enzymolysis 2 hours under the conditions of temperature 70 C.Wherein, the pectin in complex enzyme
Enzyme, zytase, dextranase and mannase vigor ratio be 100:60:60:60.
1200kg curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, 540kg curing enzymatic hydrolysis of soybean thin pulp and 660kg soybean is made
Slag wet basis.540kg curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation under the conditions of temperature is 60 DEG C, 100kg water content, which is made, is
55% curing enzymatic hydrolysis of soybean thickened pulp.
After 660kg soybean slag wet basis, 60kg dregs of beans and 30kg mixed bacteria liquid are mixed, in 28~30 DEG C of conditions of temperature
The 745kg fermentation residue dregs of rice are made in lower secondary fermentation 72 hours.Wherein, the lactic acid bacteria culturers number in mixed bacteria liquid: withered grass gemma
Bacillus species number: the quantity ratio of yeast seeds number is 100:20:5.
By 100kg curing enzymatic hydrolysis of soybean thickened pulp spray drying, by 745kg fermentation residue dregs of rice roller drying, the two dried object
The low antigen fermentation injection beans egg feed of 339kg is made after mixing.
One, nutrition content in the novel glycolysis injection bean feed that prepared by embodiment 1, as shown in table 1.
Nutrition content in the novel glycolysis injection bean feed of 1 embodiment 1 of table preparation
Two, the feeding effect for the novel glycolysis injection bean feed that prepared by embodiment 1
Piglet 300 of two weeks after 35 ages in days or so wean is chosen on Jinhua, Zhejiang gold sail cultivation pig farm, by weight, tire
Secondary close, the fifty-fifty principle of gender is randomly divided into 5 groups, every group of 6 repetitions, 10 pigs of each repetition.Soybean is sprayed in novel glycolysis
Feed (hereinafter referred to as injection bean feed) is directly mixed with basal diet in 4.0%, 6.0%, 8.0% and 10.0% ratio,
It is fed after additional addition, experimental period 35 days, test result was as shown in Tables 3 and 4.
3 basal diet of table composition and nutrition
Note: contain calcium citrate, calcium monohydrogen phosphate, sodium chloride, sweetener, antioxidant, lysine, egg in 4% premix
Propylhomoserin, threonine, tryptophan, phytase, composite trace element, choline, multi-vitamins, medicated premix etc..
Table 4 additionally adds novel glycolysis injection bean feed feeding experiment result
It can be seen from Table 4 that: novel glycolysis injection bean feed is in 4.0%, 6.0%, 8.0% and 10.0% ratio
Directly with basal diet mixture, fed after additional addition, the daily gain of piglet improves 4.73% than control group respectively, 7.07%,
9.87% and 9.36%；Feed intake improves 3.52%, 6.50%, 8.64% and 7.42% than control group respectively；But each group material meat
Than there are no significant change.
Diarrhea rate significantly reduces 34.33%, 48.74%, 50.48% and 58.70% than control group respectively；
The result shows that: novel glycolysis injection bean feed is directly with basal diet mixture, and additional usage amount of adding is in 4.0-
When 10.0%, the production performance of weanling pig can obviously improve, promote weanling pig growth, improve daily gain, increase feeding
Amount.
Four, bean feed feeding effect is sprayed in novel glycolysis prepared by embodiment 3
In Anhui Fengtai herd former one branch of pig farm choose body condition, parity, health binary similar in weight and the expected date of childbirth grow it is white
Milking sow 100,4 groups are randomly divided into, every group of 25 sows, to study the practical feeding of novel glycolysis injection bean feed
Bean feed group, 10.0% are sprayed in effect, i.e. control group, 3.0% novel glycolysis injection bean feed group, 5.0% novel glycolysis
Bean feed group is sprayed in novel glycolysis.Test terminates when producing the last fortnight to piglet wean in 23 days.
4 groups of test sucking pig basal diet compositions and nutrition are all the same.Control group does not add novel glycolysis injection soybean and raises
Bean feed is sprayed in material, the 3.0% novel glycolysis of novel glycolysis injection bean feed group addition 3.0%；5.0% novel glycolysis spray
Penetrate the novel glycolysis injection bean feed of bean feed group addition 5.0%；10.0% novel glycolysis injection bean feed group addition
Bean feed is sprayed in 10.0% novel glycolysis.See Table 5 for details.
It tests pig house and uses independent obstetric table, there is ventilating system on roof, and ventilation and lighting is preferable, natural lighting on daytime, ventilation,
Night lights illumination, normal immunological and management.Pig house temperature is controlled to 18 DEG C or so, and humid control is in 55-60%.It does as far as possible
It is consistent to environmental condition, it is managed with member is at a draft supported, is freely eaten and drinks water.
Table 5 tests daily ration composition and nutrition %
Note: 4% premix contains mountain flour calcium, calcium monohydrogen phosphate, salt, lysine, methionine, threonine, choline, anti-oxidant
Agent, phytase, multi-vitamins, composite trace element etc..
Influence of the bean feed to milking sow production performance is sprayed in the novel glycolysis of table 6
By table 5 and 6 it is found that spraying bean feed, 5.0% novel ferment using 3.0% novel glycolysis in lactating swine diets
Solution injection bean feed, 10.0% novel glycolysis injection bean feed feed milking sow, and the daily gain of piglet is respectively than control
Group improves 3.74%, 12.10% and 12.63% (p < 0.05), and 4.04%, 4.82% and has been respectively increased in 23 age in days survival rates
5.61% (p < 0.05).
The feed intake of milking sow improves 5.39%, 10.35% and 12.05% (p < 0.05) than control group respectively, female
Pig postweaning estrus interval shortens 12.33%, 17.81% and 24.66% (p < 0.05) than control group.The result shows that: it is new
Type glycolysis injection bean feed can significantly improve the reproductive performance of milking sow.
Above content is only citing made for the present invention and explanation, affiliated those skilled in the art are to institute
The specific embodiment of description does various modifications or additions or is substituted in a similar manner, without departing from invention
Conceive or beyond the scope defined by this claim, is within the scope of protection of the invention.
Claims (10)
1. a kind of preparation method of novel glycolysis injection bean feed, which comprises the steps of:
1), soybean is crushed plus clean water stirring is sized mixing, big soya-bean milk is made；
2) soybean stock pump, is entered Jet liquefier to cure, then liquid pump will be cured and entered after heat preservation filling is kept the temperature after cooling, addition complex enzyme
Curing enzymatic hydrolysis of soybean slurry is made in enzymatic hydrolysis；
3), curing enzymatic hydrolysis of soybean slurry is separated by solid-liquid separation, curing enzymatic hydrolysis of soybean thin pulp and soybean slag wet basis is made；
4), curing enzymatic hydrolysis of soybean thin pulp is concentrated by evaporation, curing enzymatic hydrolysis of soybean thickened pulp is made；
5) soybean slag wet basis, dregs of beans and mixed bacteria liquid, are mixed into post-fermentation, the fermentation residue dregs of rice are made；
6) dry after, mixing curing enzymatic hydrolysis of soybean thickened pulp and the fermentation residue dregs of rice, novel glycolysis injection bean feed is made.
2. the preparation method of novel glycolysis injection bean feed according to claim 1, which is characterized in that in step 1),
The weight ratio of soybean and clean water is 100:100~2000, and soybean smashing fineness is 8-160 mesh.
3. the preparation method of novel glycolysis injection bean feed according to claim 2, which is characterized in that in step 1),
The weight ratio of soybean and clean water is 100:400~600, and soybean smashing fineness is 50-60 mesh.
4. the preparation method of novel glycolysis injection bean feed according to claim 1, which is characterized in that in step 2),
Spraying curing temperature is 50-180 DEG C, and injection curing pressure is 0.02-6.0MPa；
Injection curing liquid holding temperature is 30-150 DEG C, and injection curing liquid soaking time is 3-300 minutes；
Compound-enzyme feed-stuff additive amount be 0.02~200U/g substrate butt, hydrolysis temperature be 5~80 DEG C, enzymolysis time be 0.05~
120h；
The vigor ratio of pectase, zytase, dextranase and mannase in complex enzyme is 100:0.05~500:
0.05~500:0.05~500.
5. the preparation method of novel glycolysis injection bean feed according to claim 4, which is characterized in that in step 2),
Spraying curing temperature is 100-120 DEG C, and injection curing pressure is 0.4-0.6MPa；
Injection curing liquid holding temperature is 70-90 DEG C, and injection curing liquid soaking time is 20-40 minutes；
Compound-enzyme feed-stuff additive amount is 4~8U/g substrate butt, and hydrolysis temperature is 50~70 DEG C, and enzymolysis time is 0.5~2h；
The vigor ratio of pectase, zytase, dextranase and mannase in complex enzyme be 100:60~80:40~
60:40~60.
6. the preparation method of novel glycolysis injection bean feed according to claim 1, which is characterized in that in step 4),
Being concentrated by evaporation temperature is 40-80 DEG C, and soymilk concentrate water content is 40-80%.
7. the preparation method of novel glycolysis injection bean feed according to claim 6, which is characterized in that in step 4),
Being concentrated by evaporation temperature is 50-70 DEG C, and soymilk concentrate water content is 55-65%.
8. the preparation method of novel glycolysis injection bean feed according to claim 1, which is characterized in that in step 5),
The weight ratio of soybean slag wet basis, dregs of beans and mixed bacteria liquid is 100:10~1000:0.5~100, and fermentation temperature is 5~50 DEG C,
Fermentation time is 2~240h；
Lactic acid bacteria culturers number in mixed bacteria liquid: Bacillus subtilis strain number: the quantity ratio of yeast seeds number is 100:0
~500:0~500.
9. the preparation method of novel glycolysis injection bean feed according to claim 8, which is characterized in that in step 5),
The weight ratio of soybean slag wet basis, dregs of beans and mixed bacteria liquid is 100:50~80:5~20, and fermentation temperature is 28~30 DEG C, fermentation
Time is 48~60h,
Lactic acid bacteria culturers number in mixed bacteria liquid: Bacillus subtilis strain number: the quantity ratio of yeast seeds number is 100:
20~30:5~10.
10. the preparation method of novel glycolysis injection bean feed according to claim 1, which is characterized in that in step 6),
It is mixed after curing enzymatic hydrolysis of soybean thickened pulp and the fermentation residue dregs of rice are separately dried, novel glycolysis injection bean feed is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811384755.0A CN109430529A (en) | 2018-11-20 | 2018-11-20 | A kind of preparation method of novel glycolysis injection bean feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811384755.0A CN109430529A (en) | 2018-11-20 | 2018-11-20 | A kind of preparation method of novel glycolysis injection bean feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109430529A true CN109430529A (en) | 2019-03-08 |
Family
ID=65553316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811384755.0A Pending CN109430529A (en) | 2018-11-20 | 2018-11-20 | A kind of preparation method of novel glycolysis injection bean feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109430529A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1759731A (en) * | 2005-11-11 | 2006-04-19 | 郑为完 | Method for preparing complete soluble instant powder of green bean |
| CN102488085A (en) * | 2011-12-15 | 2012-06-13 | 安徽基石生物工程股份有限公司 | Method of making fodder for young livestock |
| CN102919529A (en) * | 2012-11-24 | 2013-02-13 | 安徽五粮泰生物工程股份有限公司 | Preparation method of glycolysis and emulsification feed for young livestock and poultry |
| CN202819529U (en) * | 2012-10-08 | 2013-03-27 | 杭州远山食品科技有限公司 | Steam jet type soybean anti-nutritional factor inactivation device |
-
2018
- 2018-11-20 CN CN201811384755.0A patent/CN109430529A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1759731A (en) * | 2005-11-11 | 2006-04-19 | 郑为完 | Method for preparing complete soluble instant powder of green bean |
| CN102488085A (en) * | 2011-12-15 | 2012-06-13 | 安徽基石生物工程股份有限公司 | Method of making fodder for young livestock |
| CN202819529U (en) * | 2012-10-08 | 2013-03-27 | 杭州远山食品科技有限公司 | Steam jet type soybean anti-nutritional factor inactivation device |
| CN102919529A (en) * | 2012-11-24 | 2013-02-13 | 安徽五粮泰生物工程股份有限公司 | Preparation method of glycolysis and emulsification feed for young livestock and poultry |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101627793B (en) | Industrial production method of hydrolyzed wheat protein for feeding | |
| CN105053552B (en) | A kind of high bioactivity fermented bean dregs and the preparation method and application thereof | |
| CN103461687B (en) | Preparation method of fermented Chinese herbal medicine lactagogue fodder for sows in lactation period | |
| CN104509746A (en) | Preparation method of lactating sow feed | |
| CN109123093A (en) | A kind of mixed fermentation miscellaneous dregs of rice and its production technology and application | |
| CN103156057A (en) | Microbial fermented feed and production process thereof | |
| CN108835387A (en) | A kind of preparation method of functionality glycolysis feather meal feed | |
| CN102783582A (en) | Preparation method of sea-tangle, fish meal, brown sugar and ormosia fermented feed for lactating sows | |
| CN103875982A (en) | Preparation method for constipation-resisting fermented feed | |
| CN108522869A (en) | A kind of environmentally friendly sucking pig mixed feed and preparation method thereof based on guarantee enteron aisle | |
| CN104206692A (en) | Preparation method of attractant anti-constipation fermented feed | |
| CN101018488B (en) | Stabilized pancreas product | |
| CN105994946A (en) | Preparation method of high calcium lactate fermented feed | |
| CN105360615A (en) | Fermented feed raw material preparation as well as preparation method and application thereof | |
| CN107535699A (en) | A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe | |
| CN105558302A (en) | Palm meal protein feed additive and preparation method thereof | |
| CN108077610A (en) | A kind of agent for rearing and fattening meat sheep phase feed | |
| CN106071104A (en) | A kind of expanded fermentation protein feedstuff additive and application | |
| CN107373027A (en) | One kind is born to 2 week old suckling pig formulation liquid feeds and its application | |
| CN109628538A (en) | A kind of method that biomimetic method prepares functional protein polypeptide | |
| CN104686790A (en) | Preparation method of peptidoglycolipid for feed | |
| CN105410403A (en) | Preparation method of novel food calling constipation-resisting fermented feed | |
| CN106212944A (en) | A kind of Weanling pig compound feed containing low antigen fermented bean cake and preparation method | |
| CN109430530A (en) | A kind of preparation method of injection curing liquefaction beans paddy glycolysis feed | |
| CN105767556A (en) | Method for preparing egg-milk feed for porkers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190308 |
|
| RJ01 | Rejection of invention patent application after publication |