CN109395052A - Composition and its preparation method and application for inhibiting growth of tumour cell - Google Patents
Composition and its preparation method and application for inhibiting growth of tumour cell Download PDFInfo
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- CN109395052A CN109395052A CN201811324678.XA CN201811324678A CN109395052A CN 109395052 A CN109395052 A CN 109395052A CN 201811324678 A CN201811324678 A CN 201811324678A CN 109395052 A CN109395052 A CN 109395052A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 19
- 230000012010 growth Effects 0.000 title claims abstract description 18
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 15
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- GSSMIHQEWAQUPM-AOLPDKKJSA-N ovalbumin peptide Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)[C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C1=CN=CN1 GSSMIHQEWAQUPM-AOLPDKKJSA-N 0.000 claims abstract description 26
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- 238000009835 boiling Methods 0.000 claims description 27
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- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8994—Coix (Job's tears)
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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Abstract
The present invention provides a kind of compositions and its preparation method and application for inhibiting growth of tumour cell.It includes the component of following parts by weight: 0.1~5 part of Caulis et Folium Brassicae capitatae, 1~20 part of Semen Coicis extract and 1~30 parts by weight ovalbumin peptide that this, which is used to inhibit the composition of growth of tumour cell,.The present invention is effectively cooperated using the ovalbumin peptide of special ratios with Caulis et Folium Brassicae capitatae and Semen Coicis extract, and the growth of tumour cell can be effectively inhibited.
Description
Technical field
The present invention relates to anti-tumor drug technical fields, more particularly, to a kind of for inhibiting growth of tumour cell
Composition and its preparation method and application.
Background technique
Cancer is a kind of disease for seriously threatening human life and health.Modern medicine is mainly to the treatment of cancer at present
Operative treatment cooperates Radiotherapy chemotherapy, though operation can remove primary lesion, cannot fundamentally prevent the regeneration of cancer cell in numerous
It grows；Though Radiotherapy chemotherapy can kill cancer cell, but it is also a large amount of normal tissue cell is damaged, gastrointestinal reaction, bone are induced
Marrow inhibits and liver kidney, impairment of cardiac function, keeps the body of patient weaker, it is difficult to further treatment.And Chinese traditional treatment cancer
There is long history, forms oneself unique theoretical system and therapeutic rules.Pass through the work of vast medical worker in recent years
Traditional Chinese medicine treating cancer is had proven to effect outstanding, important work especially has been played to inhibition cancer cell growth
With.
Western indigo plant flower also known as Broccoliflorets, broccoli etc., are in Cruciferae Btassica brassica specie using green bouquet as product
One mutation.Studies have shown that broccoli is effective against breast cancer and colorectal cancer.It is merely up to not by edible broccoli section vegetables
To the effect for inhibiting cancer cell growth.Semen coicis is the dry mature kernal of gramineae, has hypoglycemic, resists and swell
Tumor improves the effects of immunity, is clinically mainly used for oedema, tinea pedis, difficult urination, splenasthenic diarrhea, the diseases such as cancerous swelling.In reality
In use, antitumor the effect of playing of broccoli and semen coicis, is faint.
Summary of the invention
The first object of the present invention is to provide a kind of for inhibiting the composition of growth of tumour cell.The composition includes
The component of following parts by weight: 0.1~5 parts by weight Caulis et Folium Brassicae capitatae, 1~20 parts by weight Semen Coicis extract and 1~30 weight
Part ovalbumin peptide.
The present invention is effectively cooperated using the ovalbumin peptide of special ratios with Caulis et Folium Brassicae capitatae and Semen Coicis extract, can
Effectively to inhibit the growth of tumour cell.
In a preferred embodiment of the invention, above-mentioned composition includes the component of following parts by weight: 0.5~2 weight
The blue flower extract in part west, 5~10 parts by weight Semen Coicis extracts and 5~15 parts by weight ovalbumin peptides.It is further preferably,
Component including following parts by weight: the blue flower extract in 1~2 parts by weight west, 6~8 parts by weight Semen Coicis extracts and 6~10 weight
Part ovalbumin peptide.
In the present invention, parts by weight can be the unit of weight well known in the art such as μ g, mg, g, kg, be also possible to it again
Number, such as 1/10,1/100,10 times, 100 times.In the present invention, it is preferred to be kg.
In a preferred embodiment of the invention, the preparation method of the ovalbumin peptide include the following steps: using
Water and alkali protease extract the ovalbumin peptide from ovalbumin.
The water that 5~20 times of its quality is added in ovalbumin is more particularly: subjected to 40 DEG C~60 DEG C of heating, mashing；It will
Feed liquid after mashing is adjusted to pH 7~9；It is incorporated as the alkali protease of ovalbumin weight 3~5%, carries out enzyme digestion reaction；
The enzymolysis liquid that enzymatic hydrolysis obtains is heated to boiling, inactivates 0.5~2h；60~80 DEG C are cooled to the enzymolysis liquid after inactivation, takes filter
Liquid obtains the ovalbumin peptide.Wherein, the time of enzyme digestion reaction is 5~10h, preferably 6~8h.
In a preferred embodiment of the invention, the above-mentioned blue flower extract in west is the blue flower seed water extract in west.Wherein,
The blue flower seed water extract in west the preparation method comprises the following steps: by western blue flower seed boiling to boiling, filtrate is collected by filtration.More preferably
Ground is that western blue flower seed is carried out boiling twice, the filtrate after boiling twice is collected, obtains western blue flower seed water extract.
In a preferred embodiment of the invention, Semen Coicis extract described above is semen coicis water extract.Wherein,
The semen coicis seed water extract the preparation method comprises the following steps: by the boiling of semen coicis seed to boil, filtrate is collected by filtration.More preferably
Ground is that semen coicis seed is carried out boiling twice, the filtrate after boiling twice is collected, semen coicis seed water extract is obtained.
Broccoli extract powder and semen coicis extract powder can be used in the present invention and ovalbumin Gly-His-Lys are mixed to get group
Object is closed, when Caulis et Folium Brassicae capitatae and Semen Coicis extract are water extract, then composition may include following parts by weight
Component: 0.1~5 part of broccoli water extract, 1~20 part of semen coicis water extract and 1~30 parts by weight ovalbumin peptide.
In a preferred embodiment of the invention, the active constituent of composition is extracted by Caulis et Folium Brassicae capitatae, semen coicis
Object and ovalbumin peptide composition.
Composition of the invention can be used method commonly used in the art and be mixed to prepare in proportion.
The second object of the present invention is to provide the preparation method of above-mentioned composition, which includes the following steps:
By semen coicis seed, the blue flower seed in west, respectively filtrate is collected by filtration to boiling in boiling, obtains Semen Coicis extract respectively
With western blue flower extract；
The Semen Coicis extract, the blue flower extract in west and the ovalbumin peptide are mixed, mixed liquor is subjected to low temperature
It is concentrated under reduced pressure, 40~60 DEG C of temperature, 0.03~0.07MPa of pressure；180~220 DEG C of import air temperature of spray drying exports wind-warm syndrome
80~110 DEG C of degree is to get the composition.
In a preferred embodiment of the invention, the preparation method of the present composition includes the following steps:
The water that 5~20 times of its quality is added in ovalbumin is subjected to 40 DEG C~60 DEG C of heating, mashing；By the feed liquid after mashing
It is adjusted to pH 7~9；3~5% alkali proteases are added, carry out enzyme digestion reaction；The enzymolysis liquid that enzymatic hydrolysis obtains is heated to boiling,
Inactivate 0.5~2h；60~80 DEG C are cooled to the enzymolysis liquid after inactivation, filtrate is taken to obtain the ovalbumin peptide；
By semen coicis seed, the blue flower seed in west, respectively filtrate is collected by filtration to boiling in boiling, obtains Semen Coicis extract respectively
With western blue flower extract；
The Semen Coicis extract, the blue flower extract in west and the ovalbumin peptide are mixed, mixed liquor is subjected to low temperature
It is concentrated under reduced pressure, 40~60 DEG C of temperature, 0.03~0.07MPa of pressure；180~220 DEG C of import air temperature of spray drying exports wind-warm syndrome
80~110 DEG C of degree is to get the composition.
Wherein, the preparation method of ovalbumin peptide includes the following steps: using water and alkali protease from ovalbumin
Extract the ovalbumin peptide.Specifically preferred steps are shown in upper described that details are not described herein again.
The second object of the present invention is that provide above-mentioned composition or above-mentioned preparation method inhibits tumour cell raw in preparation
Application in long drug or nutraceutical.
Composition of the invention can effectively inhibit the growth of tumour cell, especially liver cancer cells.
The present invention is effectively cooperated using the ovalbumin peptide of special ratios with Caulis et Folium Brassicae capitatae and Semen Coicis extract, can
Effectively to inhibit the growth of tumour cell.
Specific embodiment
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is not intended to limit the scope of the invention.
Unless otherwise specified, the conventional means that technological means used is well known to those skilled in the art in embodiment, institute
It is commercial goods with raw material.
Embodiment 1
Present embodiments provide it is a kind of for inhibiting the composition of growth of tumour cell, by following parts by weight group be grouped
At: the blue flower water extract in 2 parts of west, 7 parts of semen coicis water extracts and 8 parts of ovalbumin peptides.
The preparation method of above-mentioned composition includes the following steps:
1) water that 15 times of its quality is added in ovalbumin is subjected to 40 DEG C of heating, mashing；Feed liquid after mashing is adjusted to
pH 7；It is incorporated as the alkali protease of ovalbumin weight 5%, carries out enzymatic hydrolysis 8h；The enzymolysis liquid that enzymatic hydrolysis obtains is heated to boiling
It rises, inactivates 0.5h；60 DEG C are cooled to the enzymolysis liquid after inactivation, filtrate is taken to obtain ovalbumin peptide；
2) semen coicis seed, the blue flower seed in west are respectively subjected to boiling twice to boiling, plate-frame filtering collects filtrate, respectively
Obtain the blue flower extract of Semen Coicis extract and west；
3) Semen Coicis extract, the blue flower extract in west and the ovalbumin peptide are mixed according to the above ratio, will be mixed
It closes liquid and carries out low-temperature reduced-pressure concentration, temperature 60 C, pressure 0.03MPa；180 DEG C of import air temperature of spray drying exports air temperature
80 DEG C to get the composition.
Embodiment 2
Present embodiments provide it is a kind of for inhibiting the composition of growth of tumour cell, by following parts by weight group be grouped
At:
The blue flower water extract in 1 part of west, 6 parts of semen coicis water extracts and 7 parts of ovalbumin peptides.Wherein, above-mentioned composition
Preparation method is identical as the preparation method of embodiment 1.
Embodiment 3
Present embodiments provide it is a kind of for inhibiting the composition of growth of tumour cell, by following parts by weight group be grouped
At:
The blue flower water extract in 2 parts of west, 8 parts of semen coicis water extracts and 10 parts of ovalbumin peptides.Wherein, above-mentioned composition
Preparation method is identical as the preparation method of embodiment 1.
Embodiment 4
Present embodiments provide it is a kind of for inhibiting the composition of growth of tumour cell, by following parts by weight group be grouped
At:
The blue flower water extract in 2 parts of west, 10 parts of semen coicis water extracts and 15 parts of ovalbumin peptides.Wherein, above-mentioned composition
Preparation method it is identical as the preparation method of embodiment 1.
Embodiment 5
Present embodiments provide it is a kind of for inhibiting the composition of growth of tumour cell, by following parts by weight group be grouped
At:
The blue flower water extract in 0.5 part of west, 5 parts of semen coicis water extracts and 5 parts of ovalbumin peptides.Wherein, above-mentioned composition
Preparation method it is identical as the preparation method of embodiment 1.
Embodiment 6
Present embodiments provide it is a kind of for inhibiting the composition of growth of tumour cell, by following parts by weight group be grouped
At:
The blue flower water extract in 5 parts of west, 20 parts of semen coicis water extracts and 30 parts of ovalbumin peptides.Wherein, above-mentioned composition
Preparation method it is identical as the preparation method of embodiment 1.
Comparative example 1
This comparative example provides a kind of composition, is grouped as by the group of following parts by weight:
2 parts of green water cress water extracts, 21 parts of semen coicis water extracts and 8 parts of ovalbumins.Wherein, the system of above-mentioned composition
Preparation Method includes the following steps:
1) green water cress seed, semen coicis seed are respectively subjected to boiling twice to boiling, plate-frame filtering collects filtrate, respectively
Obtain Semen Coicis extract and green water cress extract；
2) Semen Coicis extract, green water cress extract and ovalbumin are mixed according to the above ratio, mixed liquor is existed
Low-temperature reduced-pressure concentration is carried out under parameter commonly used in the art to get the composition.
Experimental example
Anticancer effect in vivo of the composition to mouse
Take cleaning grade ICR mouse 70, every subcutaneous traditional vaccination rat liver cancer H22 cell suspension 0.2ml of mouse right axillary.Inoculation
After for 24 hours, weighing is random to be grouped, and every group 10.It is set as lotus knurl control group, Examples 1 to 6 and 1 group of comparative example.Lotus knurl control
It organizes and presses 0.2ml/10g administered by oral gavage physiological saline daily, continuous 10 days；Examples 1 to 6 and comparative example 1 press 10mg/kg agent
Measure gastric infusion, once a day, continuous 10 times.In the 11st day execution animal, tumor tissues are taken out, weighing calculates tumour inhibiting rate.
As a result: by the Examples 1 to 6 and comparative example 1 of 10mg/kg dosage gastric infusion to rat liver cancer H22 cell
Tumour inhibiting rate is respectively 60.05%, 58.11%, 59.31%, 55.58%, 54.15%, 54.09%, 12.16%.It proves to implement
The composition that example 1~6 provides has significant Anticancer effect in vivo.
Finally, method of the invention is only preferable embodiment, it is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in protection of the invention
Within the scope of.
Claims (10)
1. a kind of for inhibiting the composition of growth of tumour cell, which is characterized in that the component including following parts by weight: 0.1~5
The blue flower extract in part west, 1~20 part of Semen Coicis extract and 1~30 parts by weight ovalbumin peptide.
2. composition according to claim 1, which is characterized in that the component including following parts by weight: 0.5~2 part of west is blue
Flower extract, 5~10 parts of Semen Coicis extracts and 5~15 parts of ovalbumin peptides.
3. composition according to claim 1 or 2, which is characterized in that the component including following parts by weight: 1~2 part of west is blue
Flower extract, 6~8 parts of Semen Coicis extracts and 6~10 parts of ovalbumin peptides.
4. composition according to any one of claim 1 to 3, which is characterized in that the preparation side of the ovalbumin peptide
Method includes the following steps: to extract the ovalbumin peptide from ovalbumin using water and alkali protease.
5. composition according to claim 4, which is characterized in that the preparation method of the ovalbumin peptide specifically include as
Lower step:
The water that 5~20 times of its quality is added in ovalbumin is subjected to 40 DEG C~60 DEG C of heating, mashing；Feed liquid after mashing is adjusted
To pH 7~9；3~5% alkali proteases are added, carry out enzyme digestion reaction；The enzymolysis liquid that enzymatic hydrolysis obtains is heated to boiling, is inactivated
0.5~2h；60~80 DEG C are cooled to the enzymolysis liquid after inactivation, filtrate is taken to obtain the ovalbumin peptide.
6. composition according to any one of claim 1 to 5, which is characterized in that the blue flower extract in west is that west is blue
Flower seed water extract；The blue flower seed water extract in west the preparation method comprises the following steps: by western blue flower seed boiling to boiling, filtering
Collect filtrate.
7. composition according to any one of claim 1 to 6, which is characterized in that the Semen Coicis extract is coix lacryma-jobi
Benevolence seed water extract；The semen coicis seed water extract the preparation method comprises the following steps: by the boiling of semen coicis seed to boil, filtering
Collect filtrate.
8. the preparation method of composition described in any one of claims 1 to 7, which comprises the steps of:
By semen coicis seed, the blue flower seed in west, respectively filtrate is collected by filtration to boiling in boiling, obtains Semen Coicis extract and west respectively
Blue flower extract；
The Semen Coicis extract, the blue flower extract in west and the ovalbumin peptide are mixed, mixed liquor is subjected to low-temperature reduced-pressure
Concentration, 40~60 DEG C of temperature, 0.03~0.07MPa of pressure；180~220 DEG C of import air temperature of spray drying exports air temperature 80
~110 DEG C to get the composition.
9. preparation method according to claim 8, which is characterized in that the preparation method of the composition includes following step
It is rapid:
The water that 5~20 times of its quality is added in ovalbumin is subjected to 40 DEG C~60 DEG C of heating, mashing；Feed liquid after mashing is adjusted
To pH 7~9；It is incorporated as the alkali protease of ovalbumin weight 3~5%, carries out enzyme digestion reaction；The enzymatic hydrolysis that enzymatic hydrolysis is obtained
Liquid is heated to boiling, and inactivates 0.5~2h；60~80 DEG C are cooled to the enzymolysis liquid after inactivation, filtrate is taken to obtain the ovalbumin
Peptide；
By semen coicis seed, the blue flower seed in west, respectively filtrate is collected by filtration to boiling in boiling, obtains Semen Coicis extract and west respectively
Blue flower extract；
The Semen Coicis extract, the blue flower extract in west and the ovalbumin peptide are mixed, mixed liquor is subjected to low-temperature reduced-pressure
Concentration, 40~60 DEG C of temperature, 0.03~0.07MPa of pressure；180~220 DEG C of import air temperature of spray drying exports air temperature 80
~110 DEG C to get the composition.
10. preparation method described in composition described in any one of claims 1 to 7 or claim 8 or 9 inhibits in preparation
The drug of growth of tumour cell or the application in nutraceutical.
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