CN109395011B

CN109395011B - Fructus amomi stomach-invigorating traditional Chinese medicine composition, fructus amomi stomach-invigorating wine and preparation method thereof

Info

Publication number: CN109395011B
Application number: CN201811509520.XA
Authority: CN
Inventors: 李光; 李宜航; 陈曦; 李学兰; 里二; 苏晶; 吕亚娜; 张丽霞; 罗云; 马国需
Original assignee: Institute of Medicinal Plant Development of CAMS and PUMC
Current assignee: Institute of Medicinal Plant Development of CAMS and PUMC
Priority date: 2018-12-11
Filing date: 2018-12-11
Publication date: 2021-08-06
Anticipated expiration: 2038-12-11
Also published as: CN109395011A

Abstract

A fructus amomi stomach-invigorating traditional Chinese medicine composition, fructus amomi stomach-invigorating wine and a preparation method thereof belong to the field of traditional Chinese medicine compound. The fructus amomi stomach-invigorating traditional Chinese medicine composition comprises the following components in parts by weight: 10-15 parts of fructus amomi, 0.5-1.5 parts of cattail pollen, 0.2-0.5 part of galangal, 0.2-0.5 part of clove, 0.1-0.2 part of long pepper, 2-4 parts of yam and 6-8 parts of astragalus membranaceus. The raw materials of the fructus amomi stomach-invigorating wine comprise base wine and a fructus amomi stomach-invigorating traditional Chinese medicine composition, wherein the weight ratio of the base wine to the fructus amomi stomach-invigorating traditional Chinese medicine composition is 30-60: 1. The preparation method of the amomum fruit stomach-invigorating wine comprises the following steps: soaking and/or percolating fructus Amomi traditional Chinese medicine composition for invigorating stomach with base liquor. Fructus amomi is a monarch drug, and has the effects of resolving dampness, stimulating appetite, warming spleen and stopping diarrhea. The traditional Chinese medicine components of cattail pollen, galangal, clove, long pepper, yam, astragalus and the like which have the effects of tonifying middle-jiao and Qi, warming stomach and strengthening spleen and promoting qi circulation are mutually coordinated with the amomum fruit, so that the traditional Chinese medicine composition has a more definite and effective effect on regulating spleen and stomach.

Description

Fructus amomi stomach-invigorating traditional Chinese medicine composition, fructus amomi stomach-invigorating wine and preparation method thereof

Technical Field

The invention relates to the field of traditional Chinese medicine compounds, and particularly relates to a fructus amomi stomach-invigorating traditional Chinese medicine composition, fructus amomi stomach-invigorating wine and a preparation method thereof.

Background

Fructus amomi is one of four southern medicines, and is used as a single medicinal material in the current market, and the efficacy is unclear and the fructus amomi is rich in taste and difficult to swallow if fructus amomi wine is brewed by the single medicinal material in the current market. Meanwhile, at present, the amomum villosum is mostly seasoned by adding cane sugar or honey, so that the amomum villosum is more harmful to the diabetic patients.

Disclosure of Invention

The invention aims to provide a fructus amomi stomach-invigorating traditional Chinese medicine composition, fructus amomi stomach-invigorating wine and a preparation method thereof, and aims to at least solve the technical problem that in the prior art, the effect of a single fructus amomi medicinal material is unclear.

The embodiment of the invention is realized by the following steps:

in a first aspect, the embodiment of the present invention provides a fructus amomi stomach strengthening traditional Chinese medicine composition, which comprises, by weight: 10-15 parts of fructus amomi, 0.5-1.5 parts of cattail pollen, 0.2-0.5 part of galangal, 0.2-0.5 part of clove, 0.1-0.2 part of long pepper, 2-4 parts of yam and 6-8 parts of astragalus membranaceus.

In the technical scheme, fructus amomi is used as a monarch drug, cattail pollen, astragalus and galangal are used as ministerial drugs, clove, long pepper and Chinese yam are used as adjuvant drugs, and amomum villosum, astragalus and galangal are used as the main components, so that the drug effect is seriously influenced after the loss. Fructus Amomi has effects of eliminating dampness, promoting appetite, warming spleen and relieving diarrhea. The traditional Chinese medicine components such as cattail pollen, galangal, clove, long pepper, yam, astragalus and the like which have the effects of tonifying middle-jiao and Qi, warming stomach and tonifying spleen and promoting qi circulation are mutually coordinated with the amomum fruit, so that the intestinal peristalsis function can be effectively promoted, the digestion can be promoted, and the traditional Chinese medicine composition has a more definite and effective effect on regulating spleen and stomach.

In some specific embodiments, the amomum villosum stomach-invigorating traditional Chinese medicine composition further comprises the following components in parts by weight: 5-8 parts of hawthorn and/or 6-8 parts of liquorice.

In the technical scheme, the hawthorn and the liquorice can be used as guiding drugs and have the effects of helping digestion and harmonizing drug properties. The hawthorn is sour and slightly sweet in taste, the liquorice is sweet in taste, the hawthorn and the liquorice are used as flavoring agents, and the hawthorn and the liquorice are added into the traditional Chinese medicine composition for flavoring, so that the mouthfeel of the fructus amomi wine can be obviously improved, the aftersweet taste is generated, a saccharide substance does not need to be directly added, and the fructus amomi wine can be taken by a diabetic patient. Meanwhile, the hawthorn and the liquorice have the effects of promoting digestion, strengthening the stomach, tonifying the spleen, benefiting qi and the like, so that the traditional Chinese medicine composition has a more definite and effective effect on regulating the spleen and the stomach.

In some specific embodiments, the granularity of the amomum villosum stomach-invigorating traditional Chinese medicine composition is 40-100 meshes.

In the technical scheme, the traditional Chinese medicine composition is crushed to the granularity, so that the medicine dregs can be conveniently separated while the medicine effect can be fully exerted in use. The inventor researches and discovers that if the particle size is larger than the requirement of the particle size, the effective components in the traditional Chinese medicine composition can not be quickly and sufficiently extracted, so that the processing time is long and the drug effect is not sufficiently exerted; if the particle size is smaller than the requirement of the particle size, the traditional Chinese medicine composition is difficult to separate from a liquid phase after being processed, so that the taste of the traditional Chinese medicine composition is poor.

In some embodiments, the amomum villosum is obtained by pretreatment, and the pretreatment comprises: before crushing fructus Amomi, heating fructus Amomi and alcohol under reflux, and oven drying.

In the technical scheme, the inventor researches and discovers that the fructus amomi has pungent smell and fat-soluble ingredients in the fructus amomi generate the pungent smell. By adopting the pretreatment process of heating and refluxing the alcohol and the fructus amomi, a part of components generating pungent smell in the fructus amomi can be extracted and separated, and the pungent smell of the fructus amomi is reduced. The ethanol is adopted for the pretreatment of reflux, the boiling point of the ethanol is just matched with the fat-soluble components generating pungent odor in the fructus amomi, the loss of other effective components in the fructus amomi caused by adopting other high-boiling-point solvents is avoided, and the ethanol is favorable for reducing the pungent odor of the fructus amomi through the pretreatment and simultaneously can keep the special medicinal fragrance of the fructus amomi.

In a second aspect, the present invention provides a fructus amomi stomach strengthening wine, which comprises base wine and the fructus amomi stomach strengthening traditional Chinese medicine composition in the first aspect, wherein the weight ratio of the base wine to the fructus amomi stomach strengthening traditional Chinese medicine composition is 30-60: 1.

In the technical scheme, the fructus amomi stomach-invigorating traditional Chinese medicine composition is infused by the base liquor, the alcohol in the base liquor can effectively extract the effective components in the fructus amomi stomach-invigorating traditional Chinese medicine composition, and the infused fructus amomi stomach-invigorating liquor taking the base liquor in the form of the medicinal liquor is also beneficial to the absorption of the effective components in the fructus amomi stomach-invigorating traditional Chinese medicine composition by a human body, and the fructus amomi stomach-invigorating liquor has high content of the effective components, good drug effect absorption and good efficacy.

In some specific embodiments, the alcohol content of the base wine is 35 to 55% vol.

In the technical scheme, the alcohol content is 35-55% vol for infusion, so that the effective ingredients in the fructus amomi stomach-invigorating traditional Chinese medicine composition are effectively extracted, and the influence on the taking experience due to too heavy medicinal flavor is avoided.

In some specific embodiments, the base wine is selected from any one or more of sake, kaoliang spirit, miscellaneous grain spirit and corn spirit.

In the technical scheme, the rice wine, the sorghum wine, the coarse cereal wine and the corn wine are wide in source and good in taste. Meanwhile, the base wines are grain wines which are rich in trace elements, multiple vitamins and the like, have a certain gastrointestinal conditioning effect and are beneficial to further improving the effect of the fructus amomi stomach-invigorating wine on regulating the spleen and stomach.

In a third aspect, an embodiment of the present invention provides a method for preparing amomum villosum stomach-invigorating wine as in the embodiment of the second aspect, including: soaking and/or percolating fructus Amomi traditional Chinese medicine composition for invigorating stomach with base liquor.

In the technical scheme, the base liquor is used for soaking and/or percolating the amomum villosum stomach-invigorating traditional Chinese medicine composition, the soaking mode is simple to operate, and the drug effect is fully extracted; the medicinal liquor and the dregs of a decoction are fully separated by a percolation mode; when the mode of combining soaking and percolation is adopted, soaking is carried out first, and then percolation is carried out, so that the medicinal liquor and the medicinal dregs are fully separated while the medicinal effect is fully extracted, and the medicinal effect and the taste are both considered.

In some specific embodiments, the method for preparing amomum villosum stomach-invigorating wine as in the examples of the second aspect comprises: step a, soaking the fructus amomi stomach-invigorating traditional Chinese medicine composition by base liquor which is 1.5-3 times of the weight of the fructus amomi stomach-invigorating traditional Chinese medicine composition to obtain prepreg; and b, percolating the prepreg by using the residual base liquor.

In the technical scheme, the fructus amomi is soaked firstly, so that the traditional Chinese medicinal materials of the fructus amomi stomach-invigorating traditional Chinese medicine composition are fully soaked and expanded firstly, and after the pre-soaking treatment, the extraction efficiency of the effective components during the percolation treatment, particularly in the earlier stage of the percolation treatment, can be improved, the percolation efficiency is improved, and the percolation time is saved.

In some specific embodiments, the soaking time in step a is 1-2 h; and/or, the percolation speed in the step b is 0.8-1.5 ml/min.

In the technical scheme, the soaking time is 1-2h, if the soaking time is short, the medicinal materials are wetted and the imbibition is not sufficient, so that the percolation effect is influenced; the medicinal materials almost reach a saturated state after imbibition to a certain degree, and the soaking time has little or no effect on improving the percolation effect, but increases the process period and the production cost. The percolation speed is 0.8-1.5ml/min, the medicine effect is not fully extracted when the percolation is too fast, the extraction effect is obviously reduced when the content of the effective components in the base wine is too high, the effect of improving the extraction rate of the effective components is slight when the percolation is too slow, and the process period and the production cost are increased on the contrary.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

Fig. 1 is a flow chart of a preparation method of fructus amomi stomach-invigorating wine provided by the embodiment of the invention;

FIG. 2 is a graph showing the statistical data of the effect of intestinal motility in mice, a model of intestinal motility inhibition in the experimental part of animals in the experimental example of the present invention;

FIG. 3 is a proportion chart of favorite persons in the compound fructus Amomi wine for invigorating stomach prepared in example 5 and the single fructus Amomi wine for invigorating stomach prepared in the comparative example according to the part of sensory evaluation in the experimental example of the present invention;

FIG. 4 is a diagram of the reason investigation and the population ratio of the fructus Amomi stomachic wine prepared in example 5 of the present invention;

fig. 5 is a proportion chart of the number of people and the reason investigation of the sensory evaluation part of the experimental example of the invention on the dislike of the amomum villosum stomach-invigorating wine prepared in the comparative example 1.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The amomum fruit stomach-invigorating traditional Chinese medicine composition, the amomum fruit stomach-invigorating wine and the preparation method thereof of the embodiment of the invention are specifically explained below.

Or 11 to 14 parts of amomum villosum, 0.8 to 1.2 parts of cattail pollen, 0.25 to 0.45 part of galangal, 0.25 to 0.45 part of clove, 0.12 to 0.18 part of long pepper, 2.5 to 3.5 parts of yam and 6.5 to 7.5 parts of astragalus.

Or fructus Amomi 12-13 parts, pollen Typhae 0.9-1.1 parts, rhizoma Alpiniae Officinarum 0.3-0.4 parts, flos Caryophylli 0.3-0.4 parts, fructus Piperis Longi 0.14-0.16 parts, rhizoma Dioscoreae 2.8-3.2 parts, and radix astragali 6.8-7.2 parts.

Such as but not limited to: 10 parts of fructus amomi, 0.5 part of cattail pollen, 0.2 part of galangal, 0.2 part of clove, 0.1 part of long pepper, 2 parts of Chinese yam and 6 parts of astragalus membranaceus.

13 parts of fructus amomi, 1 part of cattail pollen, 0.35 part of galangal, 0.35 part of clove, 0.15 part of long pepper, 3 parts of Chinese yam and 7 parts of astragalus membranaceus.

15 parts of fructus amomi, 1.5 parts of cattail pollen, 0.5 part of galangal, 0.5 part of clove, 0.2 part of long pepper, 4 parts of Chinese yam and 8 parts of astragalus membranaceus.

In some optional embodiments, the amomum villosum stomach-invigorating traditional Chinese medicine composition further comprises the following components in parts by weight: 5-8 parts of hawthorn and/or 6-8 parts of liquorice, or 5.5-7.5 parts of hawthorn and/or 6.5-7.5 parts of liquorice, or 6-7 parts of hawthorn and/or 6.8-7.2 parts of liquorice, such as but not limited to: 5 parts of hawthorn and/or 6 parts of liquorice, 6.5 parts of hawthorn and/or 7 parts of liquorice, and 8 parts of hawthorn and/or 8 parts of liquorice.

In the above embodiment, the amomum villosum stomach-invigorating traditional Chinese medicine composition may also include only hawthorn, only licorice, or both hawthorn and licorice. It should be noted that "and/or" in the present application, such as "scheme a and/or scheme B" means that the three modes of scheme a alone, scheme B alone, scheme a plus scheme B may be used.

In some specific embodiments, the fructus amomi stomach-invigorating traditional Chinese medicine composition has a lower limit of the dosage of other components and the fructus crataegi and the liquorice, and the fructus amomi stomach-invigorating traditional Chinese medicine composition has an upper limit of the dosage of other components and the fructus crataegi and the liquorice.

For example, in some specific embodiments, the amomum fruit stomach-invigorating traditional Chinese medicine composition comprises the following components in parts by weight: 10 parts of fructus amomi, 0.5 part of cattail pollen, 0.2 part of galangal, 0.2 part of clove, 0.1 part of long pepper, 2 parts of yam, 6 parts of astragalus mongholicus, 5 parts of hawthorn and 6 parts of liquorice, or 13 parts of fructus amomi, 1 part of cattail pollen, 0.35 part of galangal, 0.35 part of clove, 0.15 part of long pepper, 3 parts of yam, 7 parts of astragalus mongholicus and 6.5 parts of hawthorn and 7 parts of liquorice, or 15 parts of fructus amomi, 1.5 parts of cattail pollen, 0.5 part of galangal, 0.5 part of clove-0.5 part, 0.2 part of long pepper, 4 parts of yam, 8 parts of astragalus mongholicus, 8 parts of hawthorn and 8 parts of liquorice.

The stomach-invigorating traditional Chinese medicine composition containing fructus amomi comprises the following components and main effects:

sha ren, pungent in property and warm in flavor, enters spleen, stomach and kidney meridians, and can resolve dampness, stimulate appetite, warm spleen, check diarrhea, regulate qi and prevent abortion.

Cattail pollen, sweet in nature and taste, neutral in nature and flavor, enters liver and pericardium channels, stops bleeding, removes blood stasis and relieves stranguria.

Galangal, pungent in flavor and hot in property, enters spleen and stomach meridians, warms stomach to dispel cold, promotes digestion to alleviate pain.

Clove, pungent in nature and taste and warm in nature, enters spleen, stomach, lung and kidney meridians, warms middle energizer to descend adverse qi, tonifies kidney and invigorates yang.

Long Pepper, pungent and hot in nature and flavor, enters stomach and large intestine channels, warms middle-jiao to dispel cold, and releases qi to relieve pain.

Yam, sweet in nature and taste, has effects of invigorating spleen, lung and kidney, nourishing stomach, promoting fluid production, tonifying lung, invigorating kidney and arresting seminal emission.

Astragalus root, radix astragali, sweet in nature and taste, warm in nature, enters lung and spleen meridians, invigorates qi and strengthens exterior, induces diuresis and expels toxin, expels pus, heals wound and promotes tissue regeneration.

Haw, sour, sweet and slightly warm in nature and taste, enters spleen, stomach and liver channels, promotes digestion, invigorates stomach, promotes qi circulation and removes blood stasis.

The liquorice has sweet and mild nature and taste, and has the effects of tonifying heart, lung, spleen and stomach channels, tonifying spleen and qi, and clearing heat and removing toxicity.

In some specific embodiments, the granularity of the medicinal material in the amomum fruit stomach-invigorating traditional Chinese medicine composition is 40-100 meshes, namely that the medicinal material can pass through a 40-mesh sieve but cannot pass through a 100-mesh sieve.

In some specific embodiments, the alcohol used in the pretreatment is a high concentration alcohol, such as an alcohol having a mass fraction of not less than 95%. The reflux temperature of the pretreatment is just enough to evaporate the alcohol to reflux. The pretreatment process is carried out before the crushing of the fructus amomi, and the whole fructus amomi is treated in the pretreatment process, so that the great loss of other effective components in the fructus amomi in the pretreatment process is avoided, the pretreatment of the fructus amomi is facilitated, the pungent smell is reduced, and the special drug fragrance and the better drug effect can be kept.

In a second aspect, referring to fig. 1, the present invention provides a fructus amomi stomach strengthening wine, which comprises base wine and the fructus amomi stomach strengthening traditional Chinese medicine composition in the first aspect, wherein a weight ratio of the base wine to the fructus amomi stomach strengthening traditional Chinese medicine composition is 30-60:1, or 35-60:1, or 40-60:1, or 45-55:1, such as but not limited to 30:1, 35:1, 40:1, 45:1, 50:1, 55:1, or 60: 1.

In some specific embodiments, the alcohol content of the base wine is 35-55% vol, or 40-50% vol, such as, but not limited to, 35% vol, 40% vol, 45% vol, 50% vol, 55% vol.

In some alternative embodiments, the base wine is selected from any one or more of sake, kaoliang spirit, grain spirit and corn spirit. Wherein the coarse cereal wine is prepared by mixing and brewing multiple raw materials of coarse cereals such as sorghum, rice, glutinous rice, buckwheat, corn and the like.

In a third aspect, an embodiment of the present invention provides a method for preparing amomum villosum stomach-invigorating wine as in the embodiment of the second aspect, including: soaking and/or percolating fructus Amomi traditional Chinese medicine composition for invigorating stomach with base liquor. The brewing method of the amomum fruit stomach-invigorating traditional Chinese medicine composition by the base liquor can be only soaking extraction, can be only percolation extraction, and can also be a mode of combining soaking extraction and percolation extraction. It should be noted that: when only the soaking extraction mode is adopted, the filtration treatment is optionally carried out after the soaking extraction to separate the filtrate and the filter residue, so that the fructus amomi stomach-invigorating wine is convenient to take; when a mode of combining soaking extraction and percolation extraction is adopted, optionally, soaking extraction is firstly carried out, and then the soaked medicinal materials are subjected to percolation extraction.

In some embodiments, the preparation method of the amomum villosum stomach-invigorating wine comprises the following steps:

step a, soaking the fructus amomi stomach-invigorating traditional Chinese medicine composition by base liquor which is 1.5-3 times (or 1.5-2.5 times, such as but not limited to 1.5 times, 2 times, 2.5 times and 3 times) of the weight of the fructus amomi stomach-invigorating traditional Chinese medicine composition to obtain prepreg. And b, percolating the prepreg by using the residual base liquor. The amount of the remaining base liquor is about 30-60 times, for example 50 times in some specific embodiments, the weight of the amomum fruit stomach-invigorating Chinese medicinal composition.

In some alternative embodiments, the soaking time in step a is 1-2h, or 1.2-1.8h, or 1.4-1.6h, such as, but not limited to, 60min, 70min, 80min, 90min, 100min, 110min, 120 min. And/or, in step b, the percolation rate in step b is 0.8-1.5ml/min, or 0.9-1.4ml/min, or 1.0-1.3ml/min, or 1.1-1.2ml/min, such as but not limited to 0.8ml/min, 0.9ml/min, 1.0ml/min, 1.1ml/min, 1.2ml/min, 1.3ml/min, 1.4ml/min, 1.5 ml/min. When the soaking time in the step a is shorter, the percolation speed in the step b can be selected to be slower; when the soaking time in the step a is shorter, the percolation speed in the step b can be selected to be faster.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

A fructus amomi stomach-invigorating traditional Chinese medicine composition comprises:

10g of fructus amomi, 0.5g of cattail pollen, 0.2g of galangal, 0.2g of clove, 0.1g of long pepper, 2g of Chinese yam, 6g of astragalus mongholicus, 5g of hawthorn and 6g of liquorice.

Example 2

13g of fructus amomi, 1g of cattail pollen, 0.35g of galangal, 0.35g of clove, 0.15g of long pepper, 3g of Chinese yam, 7g of astragalus mongholicus, 6.5g of hawthorn and 7g of liquorice.

Example 3

15g of fructus amomi, 1.5g of cattail pollen, 0.5g of galangal, 0.5g of clove, 0.2g of long pepper, 4g of Chinese yam, 8g of astragalus, 8g of hawthorn and 8g of liquorice.

Example 4

A preparation method of fructus amomi stomach-invigorating wine comprises the following steps:

s1, preparing the fructus amomi stomach-invigorating traditional Chinese medicine composition according to the proportion in the embodiment 2, and preparing kaoliang spirit with the alcoholic strength of 45% vol as base wine according to the amount which is 50 times of the weight of the fructus amomi stomach-invigorating traditional Chinese medicine composition.

S2, grinding the fructus amomi stomach-invigorating traditional Chinese medicine composition to 40-100 meshes to obtain medicinal powder.

S3, soaking the medicinal powder in base liquor 2 times the weight of the medicinal powder for 1.5h, adding the residue and the medicinal liquid into a percolation device after soaking, controlling the percolation speed to be 1.2ml/min, percolating with the rest base liquor, and collecting liquid to obtain the fructus amomi stomach-invigorating wine.

Example 5

A preparation method of fructus amomi stomach strengthening wine, which is different from the embodiment 4 in that the fructus amomi is pretreated before the step of S2, and the complete fructus amomi is heated, refluxed and dried by adopting 95 percent alcohol by mass fraction.

Comparative example 1

A preparation method of fructus amomi stomach strengthening wine is characterized in that the traditional Chinese medicine raw materials only contain fructus amomi, the dosage of the fructus amomi stomach strengthening traditional Chinese medicine raw materials is the same as that of the fructus amomi stomach strengthening traditional Chinese medicine composition used in the embodiment 5, and meanwhile, sucrose is added for seasoning, and the mass percentage of the added sucrose in the fructus amomi stomach strengthening wine is 5%.

Test examples

First, animal experiment

1. Experimental methods

Preparation of 1.1, 10% carbon ink suspension

Weighing 2g of sodium carboxymethylcellulose (CMC-Na), adding 150mL of distilled water, boiling until the solution is transparent, weighing 20g of activated carbon powder, adding the activated carbon powder into the solution, boiling for 3 times, cooling the solution, metering the volume to 200mL, storing in a refrigerator at 4 ℃, and shaking uniformly before use.

1.2 preparation of test substance solution

Concentrating the fructus Amomi wine prepared in example 5 by rotary evaporation to relative density of 1.0-1.2, and keeping.

1g of the content of the cathartic capsule (a positive control group), 1g of the fructus amomi stomach-invigorating wine (a fructus amomi stomach-invigorating wine low-dose group), 2g of the fructus amomi stomach-invigorating wine medium-dose group and 4g of the fructus amomi stomach-invigorating wine high-dose group are respectively mixed with 10mL of 10 percent carbon ink suspension to prepare suspension for use.

1.3, testing the influence of the amomum fruit stomach-invigorating wine on the small intestine propulsion of the enterokinesia inhibition model mouse

Taking male Kunming mice (18-22g), randomly dividing into normal group, model group, positive control group, fructus Amomi stomach invigorating wine high dose group, fructus Amomi stomach invigorating wine medium dose group, and fructus Amomi stomach invigorating wine low dose group, each group contains 10 mice. Before the experiment, mice in each group are fasted for 20 hours without water supply, 1% CMC-Na solution is administrated in the normal group by intragastric administration, and loperamide hydrochloride solution (10mg kg) is administrated in the other groups by intragastric administration^-1) The administration volume is 20mL^-1After 30min, the normal group and the model group are intragastrically administered with 10% carbon ink suspension, the other groups of mice are intragastrically administered with 10% carbon ink suspension of the tested sample, the administration volume is 0.5 mL/mouse, and the administration dose of each group of mice of tested solution is equivalent to the maximum crude drug dose of the fructus amomi for people in the pharmacopoeia of the people's republic of China; after 25 min; cervical dislocation to sacrifice the animal; measuring the length of the intestinal canal as the total length of the small intestine; from the pylorus to the leading edge of the ink is the "ink advance length"; the ink propulsion rate is calculated according to the formula (1).

The ink propulsion rate = ink propulsion length (cm)/total small intestine length (cm) × 100%, formula (1).

1.4 statistical methods

All experimental data were statistically processed through SPSS 19.0, and compared between groups for one-way variance analysis, the data are expressed as (` x. + -. s), and P <0.05 indicates that the difference is statistically significant.

2. Results of the experiment

The effect of the solvent fructus amomi stomach-invigorating wine on the small intestine propulsion of the enterokinesia inhibition model mouse is shown in a result chart 2. As can be seen from FIG. 2, the intestinal propulsion rate of the mice in the model group is significantly lower than that in the normal group (P <0.01), which indicates that the mouse intestinal motility inhibition model is successfully prepared; compared with the model group, the small intestine propulsion rate of the positive control group mice is obviously higher than that of the model group (P <0.05), and the small intestine propulsion rate of the villous amomum fruit wine high, medium and low dose groups is obviously higher than that of the model group (P < 0.01).

As can be seen, the intestinal pushing rate is the most intuitive index for indicating the intestinal peristalsis capability of the organism, and the improvement of the intestinal pushing rate indicates the improvement of the intestinal peristalsis capability and has obvious improvement on the digestive system. The experimental results show that compared with the model group, the small intestine propulsion rate of the high, medium and low dosage group of the fructus amomi wine is obviously higher than that of the model group (P is less than 0.01), which indicates that the fructus amomi wine prepared by the formula has good pharmacodynamic activity on mice with enterokinesia inhibition models.

Second, sensory evaluation test

Questionnaires were made on the amomum villosum stomach-invigorating wine prepared in example 5 and the amomum villosum stomach-invigorating wine prepared in comparative example 1, and the preference of the investigator for the amomum villosum stomach-invigorating wine prepared in example 5 and the amomum villosum stomach-invigorating wine prepared in comparative example 1 was known. The results of the total investigation of 200 cases are shown in fig. 3 to 5, which respectively show the population ratio of the amomum fruit stomachic wine prepared in tendency example 5 to the amomum fruit stomachic wine prepared in comparative example 1 (shown in fig. 3), the reason and the population ratio of the amomum fruit stomachic wine prepared in tendency preference example 5 (shown in fig. 4), and the reason and the population ratio of the amomum fruit stomachic wine prepared in tendency preference example 5 to the amomum fruit stomachic wine prepared in comparative example 1 (shown in fig. 5).

The results show that, in 200 cases of the investigation, 195 persons are more inclined to the compound fructus amomi wine prepared in example 5, and 5 persons are more inclined to the single fructus amomi wine prepared in comparative example 1. For investigation reasons, among 195 people who prefer the compound fructus amomi wine prepared in example 5, 73 people feel that the fructus amomi in the compound fructus amomi wine has moderate taste and is easy to accept, 59 people prefer the aftertaste of the compound fructus amomi wine, 43 people feel that the compound fructus amomi wine has lasting taste and is easy to accept, and 20 people feel that the compound fructus amomi wine has no stimulation and is easier to swallow. However, in 195 people, people do not like the single-formula fructus amomi wine prepared in the comparative example 1, 89 people feel that the special taste of fructus amomi is too big and the irritation is strong, 56 people feel that the sweetness is too high and the taste is not good after sugar is added, and 50 people feel that the single-formula fructus amomi wine is not sweet and the taste is not easily accepted.

In conclusion, the fructus amomi stomach-invigorating traditional Chinese medicine composition, the fructus amomi stomach-invigorating wine and the preparation method thereof provided by the embodiment of the invention have the advantages that the fructus amomi is a monarch drug, and is capable of resolving dampness, stimulating appetite, warming spleen and stopping diarrhea. The traditional Chinese medicine components of cattail pollen, galangal, clove, long pepper, yam, astragalus and the like which have the effects of tonifying middle-jiao and Qi, warming stomach and strengthening spleen and promoting qi circulation are mutually coordinated with the amomum fruit, so that the traditional Chinese medicine composition has a more definite and effective effect on regulating spleen and stomach.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims

1. The fructus amomi stomach-invigorating traditional Chinese medicine composition is characterized by being prepared from the following raw materials in parts by weight: 10-15 parts of fructus amomi, 0.5-1.5 parts of cattail pollen, 0.2-0.5 part of galangal, 0.2-0.5 part of clove, 0.1-0.2 part of long pepper, 2-4 parts of yam and 6-8 parts of astragalus membranaceus;

the fructus amomi is obtained by pretreatment, and the pretreatment comprises the following steps: before the fructus amomi is crushed, the fructus amomi and alcohol are heated, refluxed and dried.

2. The fructus amomi stomach-invigorating traditional Chinese medicine composition as claimed in claim 1, which is characterized by further comprising the following raw materials in parts by weight: 5-8 parts of hawthorn and/or 6-8 parts of liquorice.

3. The amomum fruit stomach-invigorating traditional Chinese medicine composition as claimed in claim 1 or 2, wherein the granularity of the medicinal materials is 40-100 meshes.

4. A fructus amomi stomach strengthening wine is characterized in that raw materials of the fructus amomi stomach strengthening wine comprise base wine and the fructus amomi stomach strengthening traditional Chinese medicine composition as claimed in any one of claims 1 to 3, wherein the weight ratio of the base wine to the fructus amomi stomach strengthening traditional Chinese medicine composition is 30-60: 1.

5. The fructus amomi stomach-invigorating wine as claimed in claim 4, characterized in that the alcoholic strength of the base wine is 35-55% vol.

6. The amomum villosum stomach-invigorating wine as claimed in claim 4, wherein the base wine is one or more selected from rice wine, kaoliang spirit, miscellaneous grain spirit and corn spirit.

7. A method for preparing the amomum villosum stomach-invigorating wine as claimed in any one of claims 4 to 6, which comprises the following steps: soaking and/or percolating the fructus amomi stomach-invigorating traditional Chinese medicine composition by adopting the base wine.

8. The method of claim 7, comprising:

step a, soaking the fructus amomi stomach-invigorating traditional Chinese medicine composition by adopting the base wine with the weight 1.5-3 times of that of the fructus amomi stomach-invigorating traditional Chinese medicine composition to obtain a prepreg;

and b, percolating the prepreg by using the residual base liquor.

9. The method according to claim 8, wherein the soaking time in step a is 1-2 h;

and/or, the percolation speed in the step b is 0.8-1.5 ml/min.