CN109394944A - Compound containing strong medicine iron holly bark and rhizoma cyperi - Google Patents
Compound containing strong medicine iron holly bark and rhizoma cyperi Download PDFInfo
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- CN109394944A CN109394944A CN201811535025.6A CN201811535025A CN109394944A CN 109394944 A CN109394944 A CN 109394944A CN 201811535025 A CN201811535025 A CN 201811535025A CN 109394944 A CN109394944 A CN 109394944A
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Abstract
The present invention relates to a kind of compounds containing strong medicine iron holly bark and rhizoma cyperi, iron holly bark is mixed with rhizoma cyperi according to the weight ratio of 15-170:15-50, obtain the compound containing iron holly bark, the compound is added in animal and fowl fodder according to the additive amount of 1-10g/kg feed and is fed, is used continuously 3-7 days.The present invention can treat bacteremia caused by the drug-fast bacteria of antibiotic ineffective, and drug cost and medicament residue can be greatly reduced, and create higher economic benefit for raiser, and ensure the food safety of the mankind.
Description
Technical field
The invention belongs to bacteremia treatment of infection technical fields caused by Escherichia coli, more particularly to containing strong medicine iron holly bark and
The compound of rhizoma cyperi.
Background technique
After bacteremia refers to that extraneous bacterium enters hematological system via the entrance of body surface or the entrance of infection, in body
It breeds in blood and is sent out with blood flow in whole body, be the extensive inflammation reaction of organism infection, be the hair of global intensive care unit
The main reason for sick rate and the death rate；And the problem that China or even whole world Severe acute disease field generally face.In drug resistance
It is undoubtedly even more to make the matter worse for the ward ICU, in many all septicemia of document report hospital under the overall situation that bacterium is increased
In the case of generation, the bacteremia because of caused by antibiotic-resistance E. coli infection accounts for 40% or more unexpectedly, especially because producing super wide spectrum β-
The bacteremia death rate caused by lactamase (extended-spectrum β-lactamase, ESBL) antibiotic-resistance E. coli is more
It is high.Escherichia coli are common conditioned pathogens, and a kind of disease often sent out, it primary can cause chicken acute sepsis, abdomen
The multiple locations severe totals such as film inflammation, hepatitis, pneumonia, enteritis infection, also can it is secondary or it is mixed hair in virosis, caused to aquaculture huge
Huge economic loss.Amoxicillin, ampicillin, Ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/
The beta-lactam nucleus class antibiotic such as Sulbactam is the common important drugs for preventing and treating the disease, as cultivation scale intensive degree is cured
Come more greatly, medication is more and more spread unchecked, and antibody-resistant bacterium is more and more, and the report about Drug Resistance of E. coli is also more.Research
Confirm, drug resistant main mechanism first is that generate beta-lactamase, especially extended spectrumβ-lactamase (ESBLs), the enzyme energy
It is relatively strong and rapidly hydrolyze beta-lactam nucleus, so that this kind of antibiotic is lost antibacterial activity.ESBLs is the main of beta-lactamase
Enzyme type, it is in that intersection is resistance to aminoglycoside, quinolones and sulfamido not only to cephalo three generations and aztreonam drug resistance
Medicine, only to Carbapenems, mould alkenes medicaments insensitive.Many documents it has been reported that Escherichia coli multi-drug resistant and generation
ESBLs has substantial connection, more pays close attention to it both at home and abroad.Inventor has carried out the inspection of food animal sources pathogenic bacteria ESBLs recently
Survey, extraction, enzyme are to researchs such as the suppression enzyme protection effects of percent hydrolysis and Sulbactam, Tazobactam Sodium and Chinese medicine of antibiotic, to portion
The drug sensitive test of ESBLs Escherichia coli is divided to show that its multidrug resistant rate is apparently higher than non-ESBLs bacterial strain.In addition, result also shows
ESBLs bacterial strain is produced not only to the serious drug resistance of the third generation cephalosporins such as Ceftiofur, but also also to fluoroquinolones, aminoglycoside
A variety of antimicrobial drug resistances such as class and sulfamido, phosphonomycin are in serious multidrug resistant.Inventor separates in producing ESBLs bacterial strain
The Escherichia coli that fosA3 drug resistant gene is produced to resistance to phosphonomycin, more strengthen the drug resistance of bacterium.The drug resistance of bacterium causes to cultivate
Drug cost increases when bacteremia disease caused by drug-fast bacteria is treated at family, and treatment course extends, and mortality of livestock increases, to production
Larger economic loss is brought, and increases livestock and poultry medicament residue risk.Therefore, a kind of pair is needed in actual production regardless of whether resistance to
The Escherichia coli of medicine can have a drug of preferable therapeutic effect, reduce the death rate, reduce the green of economic loss and drug residue free
Color product, improves the quality that food animal is delivered for sale, creates higher economic benefit for raiser, also ensures the health peace of the mankind
Entirely.
Summary of the invention
Animal bacteremia disease is treated using iron holly bark and rhizoma cyperi the technical problem to be solved in the present invention is to provide a kind of
Method, it can be killed regardless of whether drug resistant Escherichia coli, overcome antimicrobial to treat the curative effect as caused by drug resistance problems
Difference, dosage are big, residual is high and defect at high cost, avoid simple Chinese medicine sterilizing and compose problem narrow, that sterilization is slow.
In order to solve the above technical problems, the invention adopts the following technical scheme: a kind of answering containing strong medicine iron holly bark and rhizoma cyperi
Square composition is made of the raw material of following parts by weight: 15-50 parts of rhizoma cyperi, 15-170 parts of iron holly bark.
Preferably, it is made of the raw material of following parts by weight: 30 parts of rhizoma cyperi, 90 parts of iron holly bark.
Preferably, the compound is prepared into powder, tablet, oral solution or granule.
Preferably, the powder the preparation method comprises the following steps: by iron holly bark and rhizoma cyperi be crushed to 20-100 mesh, mix,
To obtain the final product.
Preferably, the preparation method of the tablet is the following steps are included: iron holly bark and rhizoma cyperi are crushed, addition lubricates
Agent, binder, after mixing, granulation, tabletting to get.
Preferably, to mention 2 small the following steps are included: iron holly bark and rhizoma cyperi are carried out water for the preparation method of the oral solution
When, concentration, be diluted with water, concentration be 10% to get.
Preferably, the granule preparation method the following steps are included: by it is described iron holly bark and rhizoma cyperi are crushed,
It adds lubricant, binder, pelletizes to obtain the final product.
Preferably, the lubricant is 65% ethyl alcohol.
Preferably, the binder is 5.5% corn starch paste.
For drug resistance problems existing for current livestock and poultry coli-infection, inventor utilizes Guangxi Special Traditional Chinese Medicine iron holly bark
Compound is formed with rhizoma cyperi.Iron holly bark document report focuses mostly in treatment enteritis, diarrhea disease, to systemic bacterial infections
Bacteremia but have not been reported；This laboratory research prove iron holly bark can treat bacteremia very well, especially with polygamy 5 of comforming
After the soothing liver-qi stagnation medicine rhizoma cyperi filtered out in drug, whether the cause of disease no matter infected has drug resistance, and the composition is to whole body
The bacteremia of infection has better effect than single medical instrument.If pressing common theory of traditional Chinese medical science, systemic infection uses a large amount of bitter colds
Medicine such as iron holly bark can achieve better effect, but this discovery, in the case where Isodose, iron holly bark compatibility rhizoma cyperi is than single
Pure iron holly bark therapeutic effect is more preferable, therefore passes through to test and determine that iron holly bark compatibility rhizoma cyperi treatment bacteremia is preferable compatibility.It presses
Powder, tablet, oral solution or granule is made in the compound medicine by conventional method, according to 1-10g main ingredient/kg feed additive amount
It is added in animal and fowl fodder and is fed, drug cost and medicament residue can be greatly reduced, creates higher economy for raiser
Benefit, while also ensuring food safety.
Detailed description of the invention
Fig. 1: while containing fosA3 (1) and CTX-M (2) bacterial strain；
Fig. 2: while containing CTX-M (1) and MCR-1 (2) bacterial strain
Specific embodiment
One, embodiment
Embodiment 1
It is a kind of to treat bacteremic method using iron holly bark and the compound of rhizoma cyperi, by iron holly bark and rhizoma cyperi according to
The weight ratio of 15:15 mixes, and the compound containing iron holly bark is obtained, by the compound adding according to 1g/kg feed
Dosage, which is added in animal and fowl fodder, to be fed, and is used continuously 3 days.
The compound is prepared into powder.
The preparation method of the powder be by iron holly bark and rhizoma cyperi be crushed to 20 meshes, mix to get.
Use applied to diseased chicken.
Embodiment 2
It is a kind of to treat bacteremic method using iron holly bark and the compound of rhizoma cyperi, by iron holly bark and rhizoma cyperi according to
The weight ratio of 170:50 mixes, and the compound containing iron holly bark is obtained, by the compound according to 10g/kg feed
Additive amount, which is added in animal and fowl fodder, to be fed, and is used continuously 7 days.
The compound is prepared into tablet.
The preparation method of the tablet is to crush iron holly bark and rhizoma cyperi, add 65% ethyl alcohol, 5.5% corn starch paste,
After mixing, pelletize, tabletting to get.
Use applied to sick duck.
Embodiment 3
It is a kind of to treat bacteremic method using iron holly bark and the compound of rhizoma cyperi, by iron holly bark and rhizoma cyperi according to
The weight ratio of 50:25 mixes, and the compound containing iron holly bark is obtained, by the compound adding according to 3g/kg feed
Dosage, which is added in animal and fowl fodder, to be fed, and is used continuously 4 days.
The compound is prepared into oral solution.
The preparation method of the oral solution is that iron holly bark and rhizoma cyperi are carried out water to mention 2 hours, is concentrated, is diluted with water, dense
Degree for 10% to get.
Use applied to sick goose.
Embodiment 4
It is a kind of to treat bacteremic method using iron holly bark and the compound of rhizoma cyperi, by iron holly bark and rhizoma cyperi according to
The weight ratio of 130:40 mixes, and the compound containing iron holly bark is obtained, by the compound adding according to 5g/kg feed
Dosage, which is added in animal and fowl fodder, to be fed, and is used continuously 5 days.
The compound is prepared into granule.
The granule the preparation method comprises the following steps: iron holly bark and rhizoma cyperi are crushed by described, add 65% ethyl alcohol, 5.5%
Corn starch paste is pelletized to obtain the final product.
Use applied to sick pig.
Embodiment 5
It is a kind of to treat bacteremic method using iron holly bark and the compound of rhizoma cyperi, by iron holly bark and rhizoma cyperi according to
The weight ratio of 90:35 mixes, and the compound containing iron holly bark is obtained, by the compound according to 1-10g/kg feed
Additive amount, which is added in animal and fowl fodder, to be fed, and is used continuously 3-7 days.
The compound is prepared into powder.
The preparation method of the powder be by iron holly bark and rhizoma cyperi be crushed to 20-100 mesh, mix to get.
Use applied to infected cattle.
Embodiment 6
It is a kind of to treat bacteremic method using iron holly bark and the compound of rhizoma cyperi, by iron holly bark and rhizoma cyperi according to
The weight ratio of 90:35 mixes, and the compound containing iron holly bark is obtained, by the compound according to 1-10g/kg feed
Additive amount, which is added in animal and fowl fodder, to be fed, and is used continuously 3-7 days.
The compound is prepared into powder.
The preparation method of the powder be by iron holly bark and rhizoma cyperi be crushed to 20-100 mesh, mix to get.
Use applied to sick sheep.
Two, iron holly bark and rhizoma cyperi compatibility treatment antibiotic-resistance E. coli cause bacteremia effect
Clinic collects the pathological material of disease for suffering from bacillus coli livestock and poultry, and streak inoculation is in Mai Kangkai screening and culturing medium, 37 DEG C of mistakes
Night culture, the single bacterium colony of picking continues to be separately cultured, then Gram's staining and microscopy, primarily determines as Escherichia coli, then
Identified with the bacterial biochemical assay pipe of enterobacteriaceae, determine separation of bacterial be Escherichia coli, and with fosA3, CTX-M,
The primer of the drug resistant genes such as TEM, SHV is expanded, and determines the Resistant genetype of isolated antibiotic-resistance E. coli, then returned with mouse
Experiment is returned to determine that its is pathogenic.As a result the drug-fast bacteria part bacterial strain separated contains TEM, CTX-M, fosA3, MCR-1 etc., or simultaneously
Containing there are two types of drug resistant gene such as CTX-M and fosA3, CTX-M and MCR-1, Fig. 1, Fig. 2 are seen.
After determining pathogenic bacterium, built by the method for establishing colibacillemia disease model in this laboratory
Vertical disease model.The method of the symptom and morbid state corresponding relationship established after mouse invasion after attacking poison by this laboratory
It gives a mark, 1 mouse stage by stage will be included in respectively and be grouped, screen single medicine and compound medicine respectively (according to traditional prescription
Or diagnosis and treatment based on an overall analysis of the illness and the patient's condition combination) interior therapeutic experiment, to determine the preferable drug of final therapeutic effect.It is tested by interior therapeutic,
Determine that iron holly bark+rhizoma cyperi therapeutic effect is best, although Radix Astragali in-vitro antibacterial effect is bad, relative to other strengthen immunity medicines
The protection test in vivo effect of object, Radix Astragali is relatively good, some other bitter cold antibacterials, although it is external effective, but
Septicemia caused by Escherichia coli is treated, therapeutic effect is general, may be related with reason situations such as dialectical and body absorption.Tool
Body result see the table below:
Three, the application example of compound medicine of the present invention
(1) present invention is applied to the case of chicken
Embodiment 1- embodiment 4 is prepared in accordance with the following methods: after compound iron holly bark crushed 60 meshes by a certain percentage,
As basic raw material, according to a conventional method, compound medicine is prepared into formulation products.
The formula and preparation dosage form of 2 embodiment 1-4 of table
Inventor carries out efficacy experiment by the resulting compound medicine product of above-described embodiment 1-4, in Guangxi Guigang chicken house.
Clinical symptoms: there is fragmentary death in Guangxi Guigang chicken house, 25 Day-old Broiler Chickens, chicken group, and the individual chickens of dissect are wrapped
Pericardium liver pathological change, typical bacillus coli feature.
Laboratory diagnosis: pathologic liver is taken to contaminate piece, Gram's staining, microscopically observation, it is seen that red gram-negative
Property bacillus；Tentatively it is judged as bacillus coli.It is cultivated by Escherichia coli, biochemical test identifies, and judges that chicken infection is big
Enterobacteria disease, PCR amplification, it was demonstrated that produce the ESBLs drug resistant gene for having CTX-M type.
Test therapeutic regimen: morbidity chicken group is divided into 6 groups of administrations, first group with embodiment 1 according to the addition of 1g/kg feed
Amount administration, second group is administered with embodiment 2 according to the additive amount of 2g/kg feed, and third group is with embodiment 3 according to 6g/kg feed
Additive amount administration, the 4th group with embodiment 4 according to 10g/kg feed additive amount be administered；5th group with rhizoma cyperi according to 6g/kg
The additive amount of feed is administered, and the 6th group is administered with the thick powder of iron holly bark according to the additive amount of 10g/kg feed；Above six groups of medications
Scheme persistently uses 5 days.
Wherein, chicken feed according to weight percent be corn 55.3%, dregs of beans 38%, calcium monohydrogen phosphate 1.4%, mountain flour 1%,
Salt 0.3%, oil 3%, curcuma powder 1%.
Staining effect: after first group to the 4th group of chicken group's medication, diseased chicken feed intake rises, and dead chicken no longer occurs, continues
Using 3 days, chicken group was almost recovered；For 5th group of chicken group after medication 2 days, chicken group continues to use 3 without too big marked improvement
It, chicken death phenomenon is still without being effectively controlled；For 6th group of chicken group after medication 2 days, chicken group is most still unhealthy, still has a
Other chicken death continues to use 3 days, and chicken death phenomenon is still without being effectively controlled；As it can be seen that the compound curative effect of iron holly bark
Better than folk prescription.
(2) present invention is applied to the case of duck
Embodiment 1- embodiment 4 is prepared in accordance with the following methods: after compound iron holly bark crushed 60 meshes by a certain percentage,
As basic raw material, according to a conventional method, compound medicine is prepared into formulation products.
The formula and preparation dosage form of 3 embodiment 1-4 of table
| Number | Rhizoma cyperi | Iron holly bark | Dosage form |
| Embodiment 1 | 15g | 15g | Powder |
| Embodiment 2 | 20g | 40g | Tablet |
| Embodiment 3 | 30g | 150g | Oral solution |
| Embodiment 4 | 50g | 170g | Granule |
Inventor is by the resulting compound medicine product of above-described embodiment 1-4, and in Guangxi Guigang, duck carries out efficacy experiment.
Clinical symptoms: there is fragmentary death in Guangxi Guigang duck, 25 age in days meat ducks, duck group, and the individual ducks of dissect are wrapped
Pericardium liver pathological change, typical bacillus coli feature.
Laboratory diagnosis: pathologic liver is taken to contaminate piece, Gram's staining, microscopically observation, it is seen that red gram-negative
Property bacillus；Tentatively it is judged as bacillus coli.It is cultivated by Escherichia coli, biochemical test identifies, and judges that duckling infection is big
Enterobacteria disease, PCR amplification, it was demonstrated that produce the ESBLs drug resistant gene for having CTX-M type.
Test therapeutic regimen: morbidity duck group is divided into 6 groups of administrations, first group with embodiment 1 according to the addition of 1g/kg feed
Amount administration, second group is administered with embodiment 2 according to the additive amount of 2g/kg feed, and third group is with embodiment 3 according to 6g/kg feed
Additive amount administration, the 4th group with embodiment 4 according to 10g/kg feed additive amount be administered；5th group with rhizoma cyperi according to 6g/kg
The additive amount of feed is administered, and the 6th group is administered with the thick powder of iron holly bark according to the additive amount of 10g/kg feed；Above six groups of medications
Scheme persistently uses 5 days.
The duck feed is corn 58.2%, dregs of beans 35%, calcium monohydrogen phosphate 1.4%, mountain flour according to weight percent
1.1%, salt 0.3%, oil 3%, curcuma powder 1%.
Staining effect: after first group to the 4th group of duck group's medication, sick duck feed intake rises, and dead duck no longer occurs, continues
Using 3 days, duck group was almost recovered；For 5th group of duck group after medication 2 days, duck group continues to use 3 without too big marked improvement
It, the duck phenomena of mortality are still without being effectively controlled；For 6th group of duck group after medication 2 days, duck group is most still unhealthy, still has a
Other duck is dead, continues to use 3 days, and the duck phenomena of mortality are still without being effectively controlled；As it can be seen that the compound curative effect of iron holly bark
Better than folk prescription.
(3) present invention is applied to the case of goose
Embodiment 1- embodiment 4 is prepared in accordance with the following methods: after compound iron holly bark crushed 60 meshes by a certain percentage,
As basic raw material, according to a conventional method, compound medicine is prepared into formulation products.
The formula and preparation dosage form of 4 embodiment 1-4 of table
| Number | Rhizoma cyperi | Iron holly bark | Dosage form |
| Embodiment 1 | 15g | 15g | Powder |
| Embodiment 2 | 20g | 40g | Tablet |
| Embodiment 3 | 30g | 150g | Oral solution |
| Embodiment 4 | 50g | 170g | Granule |
Inventor carries out efficacy experiment by the resulting compound medicine product of above-described embodiment 1-4, in Guangxi Guigang goose field.
Clinical symptoms: there is fragmentary death in Guangxi Guigang goose field, 25 age in days meat geese, gaggle, and the individual geese of dissect are wrapped
Pericardium liver pathological change, typical bacillus coli feature.
Laboratory diagnosis: pathologic liver is taken to contaminate piece, Gram's staining, microscopically observation, it is seen that red gram-negative
Property bacillus；Tentatively it is judged as bacillus coli.It is cultivated by Escherichia coli, biochemical test identifies, and judges that gosling infection is big
Enterobacteria disease, PCR amplification, it was demonstrated that produce the ESBLs drug resistant gene for having CTX-M type.
Test therapeutic regimen: morbidity gaggle is divided into 6 groups of administrations, first group with embodiment 1 according to the addition of 1g/kg feed
Amount administration, second group is administered with embodiment 2 according to the additive amount of 2g/kg feed, and third group is with embodiment 3 according to 6g/kg feed
Additive amount administration, the 4th group with embodiment 4 according to 10g/kg feed additive amount be administered；5th group with rhizoma cyperi according to 6g/kg
The additive amount of feed is administered, and the 6th group is administered with the thick powder of iron holly bark according to the additive amount of 10g/kg feed；Above six groups of medications
Scheme persistently uses 5 days.
The goose feed according to weight percent be corn 59.2%, wheat bran 2%, dregs of beans 22.5%, rapeseed meal 9.5%,
Calcium monohydrogen phosphate 1.3%, mountain flour 1.2%, salt 0.3%, oil 3%, curcuma powder 1%.
Staining effect: after first group to the 4th group of gaggle medication, sick goose feed intake rises, and dead goose no longer occurs, continues
Using 3 days, gaggle was almost recovered；Behind gaggle medication 2 days of 5th group, gaggle continues to use 3 without too big marked improvement
It, the goose phenomena of mortality are still without being effectively controlled；Behind gaggle medication 2 days of 6th group, gaggle majority is still unhealthy, still has a
Other goose is dead, continues to use 3 days, and the goose phenomena of mortality are still without being effectively controlled；As it can be seen that the compound curative effect of iron holly bark
Better than folk prescription.
(4) present invention is applied to the case of pig
Embodiment 1- embodiment 4 is prepared in accordance with the following methods: after compound iron holly bark crushed 60 meshes by a certain percentage,
As basic raw material, according to a conventional method, compound medicine is prepared into formulation products.
The formula and preparation dosage form of 5 embodiment 1-4 of table
| Number | Rhizoma cyperi | Iron holly bark | Dosage form |
| Embodiment 1 | 15g | 15g | Powder |
| Embodiment 2 | 20g | 40g | Tablet |
| Embodiment 3 | 30g | 150g | Oral solution |
| Embodiment 4 | 50g | 170g | Granule |
Inventor carries out efficacy experiment by the resulting compound medicine product of above-described embodiment 1-4, on Guangxi Guigang pig farm.
Clinical symptoms: there is fragmentary death in Guangxi Guigang pig farm, 25 age in days pork pigs, swinery, and the individual pigs of dissect are wrapped
Pericardium liver pathological change, typical bacillus coli feature.
Laboratory diagnosis: pathologic liver is taken to contaminate piece, Gram's staining, microscopically observation, it is seen that red gram-negative
Property bacillus；Tentatively it is judged as bacillus coli.It is cultivated by Escherichia coli, biochemical test identifies, and judges that piggy infection is big
Enterobacteria disease, PCR amplification, it was demonstrated that produce the ESBLs drug resistant gene for having CTX-M type.
Test therapeutic regimen: morbidity swinery is divided into 6 groups of administrations, first group with embodiment 1 according to the addition of 1g/kg feed
Amount administration, second group is administered with embodiment 2 according to the additive amount of 2g/kg feed, and third group is with embodiment 3 according to 6g/kg feed
Additive amount administration, the 4th group with embodiment 4 according to 10g/kg feed additive amount be administered；5th group with rhizoma cyperi according to 6g/kg
The additive amount of feed is administered, and the 6th group is administered with the thick powder of iron holly bark according to the additive amount of 10g/kg feed；Above six groups of medications
Scheme persistently uses 5 days.
Wherein pannage is corn 59%, wheat bran 13%, peanut cake (soya-bean cake) 15%, grass meal according to weight percent
(corn stalk, pachyrhizus be climing, peanut vine, alfalfa meal and green grass powder) 5%, domestic fish meal 6%, bone meal 1.5%, (cured fish powder is or not salt
Add salt) 0.5%.
Staining effect: after first group to the 4th group of swinery medication, sick pig feed intake rises, and dead pig no longer occurs, continues
Using 3 days, swinery was almost recovered；Behind swinery medication 2 days of 5th group, swinery continues to use 3 without too big marked improvement
It, the pig phenomena of mortality are still without being effectively controlled；Behind swinery medication 2 days of 6th group, swinery majority is still unhealthy, still has a
Other pig is dead, continues to use 3 days, and the pig phenomena of mortality are still without being effectively controlled；As it can be seen that the compound curative effect of iron holly bark
Better than folk prescription.
(5) present invention is applied to the case of ox
Embodiment 1- embodiment 4 is prepared in accordance with the following methods: after compound iron holly bark crushed 60 meshes by a certain percentage,
As basic raw material, according to a conventional method, compound medicine is prepared into formulation products.
The formula and preparation dosage form of 6 embodiment 1-4 of table
| Number | Rhizoma cyperi | Iron holly bark | Dosage form |
| Embodiment 1 | 15g | 15g | Powder |
| Embodiment 2 | 20g | 40g | Tablet |
| Embodiment 3 | 30g | 150g | Oral solution |
| Embodiment 4 | 50g | 170g | Granule |
Inventor carries out efficacy experiment by the resulting compound medicine product of above-described embodiment 1-4, in Guangxi Guigang cattle farm.
Clinical symptoms: there is fragmentary death in Guangxi Guigang cattle farm, 25 age in days beef cattles, cows, and the individual oxen of dissect are wrapped
Pericardium liver pathological change, typical bacillus coli feature.
Laboratory diagnosis: pathologic liver is taken to contaminate piece, Gram's staining, microscopically observation, it is seen that red gram-negative
Property bacillus；Tentatively it is judged as bacillus coli.It is cultivated by Escherichia coli, biochemical test identifies, and judges that small cattle infected is big
Enterobacteria disease, PCR amplification, it was demonstrated that produce the ESBLs drug resistant gene for having CTX-M type.
Test therapeutic regimen: morbidity cows are divided into 6 groups of administrations, first group with embodiment 1 according to the addition of 1g/kg feed
Amount administration, second group is administered with embodiment 2 according to the additive amount of 2g/kg feed, and third group is with embodiment 3 according to 6g/kg feed
Additive amount administration, the 4th group with embodiment 4 according to 10g/kg feed additive amount be administered；5th group with rhizoma cyperi according to 6g/kg
The additive amount of feed is administered, and the 6th group is administered with the thick powder of iron holly bark according to the additive amount of 10g/kg feed；Above six groups of medications
Scheme persistently uses 5 days.
Wherein ox feed is that beef cattle forage is formulated cotton dregs 15%, dregs of beans 10%, wheat bran 9%, jade according to weight percent
Rice 61%, premix 5%.
Staining effect: after first group to the 4th group of cows medication, infected cattle feed intake rises, and dead ox no longer occurs, continues
Using 3 days, cows were almost recovered；Behind cows medication 2 days of 5th group, cows continue to use 3 without too big marked improvement
It, the ox phenomena of mortality are still without being effectively controlled；Behind cows medication 2 days of 6th group, cows majority is still unhealthy, still has a
Other ox is dead, continues to use 3 days, and the ox phenomena of mortality are still without being effectively controlled；As it can be seen that the compound curative effect of iron holly bark
Better than folk prescription.
(6) present invention is applied to the case of sheep
Embodiment 1- embodiment 4 is prepared in accordance with the following methods: after compound iron holly bark crushed 60 meshes by a certain percentage,
As basic raw material, according to a conventional method, compound medicine is prepared into formulation products.
The formula and preparation dosage form of 7 embodiment 1-4 of table
| Number | Rhizoma cyperi | Iron holly bark | Dosage form |
| Embodiment 1 | 15g | 15g | Powder |
| Embodiment 2 | 20g | 40g | Tablet |
| Embodiment 3 | 30g | 150g | Oral solution |
| Embodiment 4 | 50g | 170g | Granule |
Inventor carries out efficacy experiment by the resulting compound medicine product of above-described embodiment 1-4, in Guangxi Guigang Yang Chang.
Clinical symptoms: there is fragmentary death in Guangxi Guigang Yang Chang, 25 age in days mutton sheeps, flock of sheep, and the individual sheep of dissect are wrapped
Pericardium liver pathological change, typical bacillus coli feature.
Laboratory diagnosis: pathologic liver is taken to contaminate piece, Gram's staining, microscopically observation, it is seen that red gram-negative
Property bacillus；Tentatively it is judged as bacillus coli.It is cultivated by Escherichia coli, biochemical test identifies, and judges that small sheep infection is big
Enterobacteria disease, PCR amplification, it was demonstrated that produce the ESBLs drug resistant gene for having CTX-M type.
Test therapeutic regimen: morbidity flock of sheep are divided into 6 groups of administrations, first group with embodiment 1 according to the addition of 1g/kg feed
Amount administration, second group is administered with embodiment 2 according to the additive amount of 2g/kg feed, and third group is with embodiment 3 according to 6g/kg feed
Additive amount administration, the 4th group with embodiment 4 according to 10g/kg feed additive amount be administered；5th group with rhizoma cyperi according to 6g/kg
The additive amount of feed is administered, and the 6th group is administered with the thick powder of iron holly bark according to the additive amount of 10g/kg feed；Above six groups of medications
Scheme persistently uses 5 days.
Wherein sheep feed is corn 51.9%, wheat bran 24%, peanut cake (soya-bean cake) 15%, grass meal according to weight percent
3%, domestic fish meal 4.3%, bone meal 1.3%, salt 0.5%.
Staining effect: after first group to the 4th group of flock of sheep medication, sick sheep feed intake rises, and dead sheep no longer occurs, continues
Using 3 days, flock of sheep were almost recovered；Behind flock of sheep medication 2 days of 5th group, flock of sheep continue to use 3 without too big marked improvement
It, the sheep phenomena of mortality are still without being effectively controlled；Behind flock of sheep medication 2 days of 6th group, flock of sheep majority is still unhealthy, still has a
Other sheep is dead, continues to use 3 days, and the sheep phenomena of mortality are still without being effectively controlled；As it can be seen that the compound curative effect of iron holly bark
Better than folk prescription.
It would be recognized by those skilled in the art that it is possible for making numerous accommodations to above description, so embodiment is only
For describing one or more particular implementations.
It is counted as example embodiment of the invention although having been described and describing, it will be apparent to those skilled in the art that
It can be variously modified and is replaced, without departing from spirit of the invention.Furthermore it is possible to make many modifications with will be special
Condition of pledging love is fitted to religious doctrine of the invention, without departing from invention described herein central concept.So the present invention is unrestricted
In specific embodiment disclosed herein, but the present invention may further include belonging to all embodiments of the scope of the invention and its being equal
Object.
Claims (9)
1. a kind of compound containing strong medicine iron holly bark and rhizoma cyperi, which is characterized in that be made of the raw material of following parts by weight: fragrant
It is 15-50 parts attached, 15-170 parts of iron holly bark.
2. the compound according to claim 1 containing strong medicine iron holly bark and rhizoma cyperi, which is characterized in that by following weight
The raw material composition of part: 30 parts of rhizoma cyperi, 90 parts of iron holly bark.
3. the compound according to claim 1 containing strong medicine iron holly bark and rhizoma cyperi, which is characterized in that the compound
Composition is prepared into powder, tablet, oral solution or granule.
4. the compound according to claim 3 containing strong medicine iron holly bark and rhizoma cyperi, which is characterized in that the powder
The preparation method comprises the following steps: by iron holly bark and rhizoma cyperi be crushed to 20-100 mesh, mix to get.
5. the compound according to claim 3 containing strong medicine iron holly bark and rhizoma cyperi, which is characterized in that the tablet
Preparation method the following steps are included: iron holly bark and rhizoma cyperi are crushed, addition binder, lubricant, after mixing, granulation,
Tabletting to get.
6. the compound according to claim 3 containing strong medicine iron holly bark and rhizoma cyperi, which is characterized in that described is oral
The preparation method of liquid mentions 2 hours the following steps are included: iron holly bark and rhizoma cyperi are carried out water, is concentrated, is diluted with water, concentration is
10% to get.
7. the compound according to claim 3 containing strong medicine iron holly bark and rhizoma cyperi, which is characterized in that the particle
The following steps are included: by described by iron holly bark and rhizoma cyperi crushing, addition lubricant, binder, granulation is the preparation method of agent
?.
8. the compound containing strong medicine iron holly bark and rhizoma cyperi according to claim 5 or 7, it is characterised in that: the profit
Lubrication prescription is 65% ethyl alcohol.
9. the compound containing strong medicine iron holly bark and rhizoma cyperi according to claim 5 or 7, it is characterised in that: described viscous
Knot agent is 5.5% corn starch paste.
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| CN103127507A (en) * | 2013-03-18 | 2013-06-05 | 广西大学 | Holly bark containing compound composition for treating escherichia coli infected diseases of livestock and poultry |
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| CN103127507A (en) * | 2013-03-18 | 2013-06-05 | 广西大学 | Holly bark containing compound composition for treating escherichia coli infected diseases of livestock and poultry |
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