CN109393200A - It is a kind of to increase black goldfish premix for black imperial eyeball - Google Patents

It is a kind of to increase black goldfish premix for black imperial eyeball Download PDF

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Publication number
CN109393200A
CN109393200A CN201811185209.4A CN201811185209A CN109393200A CN 109393200 A CN109393200 A CN 109393200A CN 201811185209 A CN201811185209 A CN 201811185209A CN 109393200 A CN109393200 A CN 109393200A
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premix
parts
goldfish
vitamin
black
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张蓉
朱华
王晓雯
刘丽丽
朱建亚
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Beijing Fisheries Research Institute
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Beijing Fisheries Research Institute
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
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    • AHUMAN NECESSITIES
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Abstract

To increase goldfish premix described in black goldfish premix for black imperial eyeball include tyrosine and one of vitamin premix, trace element premix agent, plant extracts pre-mixing agent or a variety of compositions the present invention relates to a kind of.The survival rate of black telescope goldfish had not only can be improved in the feed, but also can effectively increase blackness that is black or persistently guaranteeing black telescope goldfish.The ornamental values and the economic values of black telescope goldfish can be significantly provided.

Description

It is a kind of to increase black goldfish premix for black imperial eyeball
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of to increase black goldfish premix for black imperial eyeball.
Background technique
Telescope goldfish is the main goldfish breed variety in China, has higher economic value.Wherein, black telescope goldfish is with dense The black matrix color of weight is favored by people, and often the color the black more has economic value.The synthesis process of melanin specifically: Under the catalysis of tyrosinase, tyrosine is oxidized to DOPA quinone, is formed in conjunction with the intracorporal structural proteins of melanocyte after polymerization black Element.However, the black fine goldfish of dragon is in the breeding process, it is easy death, and the black of epidermis is easy to fade.
Summary of the invention
It is of the invention in order to improve the survival rate of black telescope goldfish, and can effectively increase black or persistently guarantee black telescope goldfish Blackness, provide it is a kind of increase black goldfish premix for black imperial eyeball, the goldfish premix is premixed by tyrosine and vitamin One of agent, trace element premix agent, plant extracts pre-mixing agent or a variety of compositions.
The present invention also provides a kind of feeds of black fine goldfish of dragon, including basal diet and above-mentioned goldfish premix.
Preferably, the basal diet and the amount ratio of the goldfish premix are 100:3~5;Most preferably 100:4.
The present invention increases goldfish premix on the basis of basal diet.Wherein, it especially by increase tyrosine, then ties Vitamin premix, trace element premix agent, the adjusting of three kinds of pre-mixing agents of plant extracts pre-mixing agent are closed, there is its tyrosine Appropriate oxygenation is imitated, and improves the activity of tyrosinase;Not only it can effectively improve the survival rate of black telescope goldfish, but also its black dragon can be made Eyeball goldfish forms beautiful, stable epidermis black.
Preferably, by weight, including following component: 20~30 parts of tyrosine, vitamin are pre- for the goldfish premix 1~2 part of mixture, 1~4 part of trace element premix agent, 10~25 parts of plant extracts pre-mixing agent.
More preferably: 25 parts of tyrosine, 1 part of vitamin premix, 4 parts of trace element premix agent, plant extracts premix 15~20 parts of agent.
Present invention further propose that, the vitamin premix by weight, including following component:
Vitamin A 50%:3~5%, vitamin D 50%:1~2%, vitamin E 50%:35~40%, vitamin B1 96%:0.2~0.4%, vitamin B2 78%:3~5%, vitamin B6 96%:3~5%, pantothenic acid 99%:11~ 15%, niacinamide 99%:11~15%, folic acid 98%:5~7%, biotin 2%:11~15%;
Preferably, vitamin A 50%:4%, vitamin D 50%:1.5%, vitamin E 50%:37.5%, vitamin B1 96%:0.3%, vitamin B2 78%:4%, vitamin B6 96%:4%, pantothenic acid 99%:13%, niacinamide 99%: 13%, folic acid 98%:6%, biotin 2%:13%;
Wherein, " vitamin E 50%37.5% " refers to: the vitamin E for the use of effective content being 50%, additive amount It is 37.5%.
Tyrosinase is a kind of metalloenzyme of cupric, and copper ion is the coenzyme of tyrosinase, participates in the regulation of melanogenesis. Present invention further propose that, the microelement in the trace element premix agent is selected from one of copper, iron, zinc or a variety of;
Preferably, the trace element premix agent by weight, specifically: ferrous sulfate 28%:45~50%, sulfuric acid Zinc 34%:35~37%, manganese sulfate 30%:3~3.5%, copper sulphate 25%:8~9%, calcium iodate 10%:1.2~1.7%, Sodium selenite 1%:3~3.4%;
It is furthermore preferred that ferrous sulfate 28%:48.2%, zinc sulfate 34%:35.4%, manganese sulfate 30%:3.2%, sulfuric acid Copper 25%:8.5%, calcium iodate 10%:1.5%, sodium selenite 1%:3.2%;
Wherein, " ferrous sulfate 28%:48.2% " is referred to: the content of iron is 28%, additive amount 48.2%.
Present invention further propose that, the plant extracts in the plant extracts pre-mixing agent is extracted selected from the fleece-flower root One of object, Semen Sesami Nigrum extract, Quercetin, Yeradetajo extract, Rhizoma Polygonati Odorati extract are a variety of;The plant extracts pair Tyrosinase has stronger activation, can further enhance the synthesis of melanin.
Preferably, the plant extracts in the plant extracts is alcohol extract;
It is highly preferred that the alcohol extract is specifically made in the following way:
Plant extracts uses reflux extraction: 60 DEG C of raw material being dried, is crushed, 40 meshes is crossed, then claim 10g respectively, adds The dehydrated alcohol for entering 150ml extracts in a water bath, is extracted for the first time to keep boiling reflux about 1.5h, cooled and filtered, then Novel solvent is added in the dregs of a decoction, is separately added into the dehydrated alcohol of 100ml, 500ml, carries out second, third time heating and refluxing extraction Each 0.5h, until effective component mentions to the greatest extent substantially.Merging filtrate is concentrated under reduced pressure, and alcohol constant volume makes final mass concentration 1g/mL, It is spare to be placed in 4 DEG C of refrigerators.
Present invention further propose that, the plant extracts pre-mixing agent, by weight, specifically: Semen sesami nigrum is extracted 8~12 parts of object, 4~6 parts of Quercetin, 4~6 parts of Yeradetajo extract;
Or, further including 4~6 parts of radix-polygoni multiflori extract.
Preferably, the plant extracts pre-mixing agent, by 10 parts of Semen Sesami Nigrum extract, 5 parts of Quercetin, Yeradetajo extract 5 parts;
Or, further including 5 parts of radix-polygoni multiflori extract.
A kind of any of the above-described pre-mixing agent can be selected in the present invention, also choosing multiple;When three kinds simultaneously in use, significant effect Increase.For especially tyrosine and above-mentioned three kinds of pre-mixing agents under the service condition of specific proportion, effect is especially pronounced.
Present invention further propose that, in the goldfish premix: the weight ratio of tyrosine and vitamin premix are as follows: 25:0.8~1.2, preferably 25:1;
The weight ratio of tyrosine and trace element premix agent are as follows: 25:3~5, preferably 25:4;
The weight ratio of tyrosine and plant extracts pre-mixing agent is 25:20~25, preferably 25:20.
The conventional daily ration for feeding the black fine goldfish of dragon can be used in basal diet of the present invention, organizes timesharing, base as follows when selecting The collective effect excellent of plinth daily ration and goldfish premix.
Including following component: the molten slurry albumen of dregs of beans, fish meal, wheat bran, flour, peanut meal, fish, fermented bean dregs, microlith powder, fish Oil, choline chloride, mould inhibitor;
Preferably, the basal diet, by weight, including following component: 32~36 parts of dregs of beans, fish meal 20~25 Part, 13~17 parts of wheat bran, 6~10 parts of flour, 4~6 parts of peanut meal, 2~4 parts of the molten slurry albumen of fish, 2~4 parts of fermented bean dregs, microlith 2~3 parts of powder, 1.4~1.8 parts of fish oil, 0.3~0.4 part of choline chloride, 0.04~0.06 part of mould inhibitor;
More preferably: 34.5 parts of dregs of beans, 23 parts of fish meal, 15 parts of wheat bran, 8 parts of flour, 5 parts of peanut meal, the molten slurry albumen 3 of fish Part, 3 parts of fermented bean dregs, 2.5 parts of microlith powder, 1.6 parts of fish oil, 0.35 part of choline chloride, 0.05 part of mould inhibitor.
The present invention provides a kind of preferred embodiment, and by weight, including following group is grouped as the goldfish premix:
1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, 10 parts of Semen Sesami Nigrum extract, Quercetin 5 Part, 5 parts of Yeradetajo extract;
Or, 1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, 10 parts of Semen Sesami Nigrum extract, quercitrin Plain 5 parts, 5 parts of Yeradetajo extract, 5 parts of radix-polygoni multiflori extract;
Wherein, vitamin premix: vitamin A 50%:4%, vitamin D 50%:1.5%, vitamin E 50%: 37.5%, vitamin B1 96%:0.3%, vitamin B2 78%:4%, vitamin B6 96%:4%, pantothenic acid 99%:13%, Niacinamide 99%:13%, folic acid 98%:6%, biotin 2%:13%;
Wherein, trace element premix agent: ferrous sulfate 28%:48.2%, zinc sulfate 34%:35.4%, manganese sulfate 30%: 3.2%, copper sulphate 25%:8.5%, calcium iodate 10%:1.5%, sodium selenite 1%:3.2%;
Present invention further propose that, the goldfish pre-mixing agent further includes auxiliary material, and the auxiliary material is antioxidant, powdered rice hulls Middle one or two.
Preferably, including 0.08~0.12 part of antioxidant.
Feed of the present invention is prepared using usual manner;Above-mentioned raw materials are uniformly mixed by way of mechanical mixture ?.
Feed of the present invention considerably increases the sight of black telescope goldfish while the black dragon of raising fine goldfish survival rate Reward value and economic value.There is very high market significance in terms of the cultivation of fancy fishes.
Detailed description of the invention
Fig. 1 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in comparative example 1;
Fig. 2 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in comparative example 2;
Fig. 3 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in comparative example 3;
Fig. 4 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in comparative example 4;
Fig. 5 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 1;
Fig. 6 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 2;
Fig. 7 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 3;
Fig. 8 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 4;
Fig. 9 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 5;
Figure 10 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 6;
Figure 11 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 7;
Figure 12 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 8;
Figure 13 is the aspect graph of the fine goldfish chromatophore of black dragon of forage feed described in embodiment 9;
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
The composition of basal diet and goldfish premix that following examples use:
Basal diet: 34.5 parts of dregs of beans, 23 parts of fish meal, 15 parts of wheat bran, 8 parts of flour, 5 parts of peanut meal, the molten slurry albumen 3 of fish Part, 3 parts of fermented bean dregs, 2.5 parts of microlith powder, 1.6 parts of fish oil, 0.35 part of choline chloride, 0.05 part of mould inhibitor;
Goldfish premix: 4 parts.
Following table is the nutrient content situation of basic daily ration:
Table 1
Embodiment 1
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 1.5 parts of trace element premix agent, Semen Sesami Nigrum extract 10 Part, 5 parts of Quercetin, 0.1 part of antioxidant, 82.4 parts of powdered rice hulls;
Wherein, vitamin premix: vitamin A 50%:4%, vitamin D 50%:1.5%, vitamin E 50%: 37.5%, vitamin B1 96%:0.3%, vitamin B2 78%:4%, vitamin B6 96%:4%, pantothenic acid 99%:13%, Niacinamide 99%:13%, folic acid 98%:6%, biotin 2%:13%;
Wherein, trace element premix agent: ferrous sulfate 28%:48.2%, zinc sulfate 34%:35.4%, manganese sulfate 30%: 3.2%, copper sulphate 25%:8.5%, calcium iodate 10%:1.5%, sodium selenite 1%:3.2%;
Embodiment 2
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 1.5 parts of trace element premix agent, Semen Sesami Nigrum extract 10 Part, 5 parts of Quercetin, 5 parts of Yeradetajo extract, 0.1 part of antioxidant, 77.4 parts of powdered rice hulls;
Wherein, vitamin premix: vitamin A 50%:4%, vitamin D 50%:1.5%, vitamin E 50%: 37.5%, vitamin B1 96%:0.3%, vitamin B2 78%:4%, vitamin B6 96%:4%, pantothenic acid 99%:13%, Niacinamide 99%:13%, folic acid 98%:6%, biotin 2%:13%;
Wherein, trace element premix agent: ferrous sulfate 28%:48.2%, zinc sulfate 34%:35.4%, manganese sulfate 30%: 3.2%, copper sulphate 25%:8.5%, calcium iodate 10%:1.5%, sodium selenite 1%:3.2%.
Embodiment 3
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 1.5 parts of trace element premix agent, Semen Sesami Nigrum extract 10 Part, 5 parts of Quercetin, 5 parts of Yeradetajo extract, 5 parts of radix-polygoni multiflori extract, 0.1 part of antioxidant, 72.4 parts of powdered rice hulls;
Wherein, vitamin premix: vitamin A 50%:4%, vitamin D 50%:1.5%, vitamin E 50%: 37.5%, vitamin B1 96%:0.3%, vitamin B2 78%:4%, vitamin B6 96%:4%, pantothenic acid 99%:13%, Niacinamide 99%:13%, folic acid 98%:6%, biotin 2%:13%;
Wherein, trace element premix agent: ferrous sulfate 28%:48.2%, zinc sulfate 34%:35.4%, manganese sulfate 30%: 3.2%, copper sulphate 25%:8.5%, calcium iodate 10%:1.5%, sodium selenite 1%:3.2%.
Embodiment 4
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 1.5 parts of trace element premix agent, Semen Sesami Nigrum extract 10 Part, 5 parts of Quercetin, 5 parts of Yeradetajo extract, 5 parts of Rhizoma Polygonati Odorati extract, 0.1 part of antioxidant, 72.4 parts of powdered rice hulls;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Embodiment 5
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 4 parts of trace element premix agent, 0.1 part of antioxidant, junket ammonia 25 parts, 69.9 parts of powdered rice hulls of acid;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Embodiment 6
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, Semen sesami nigrum 10 parts of extract, 5 parts of Quercetin, 0.1 part of antioxidant, 64.9 parts of powdered rice hulls;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Embodiment 7
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, Semen sesami nigrum 10 parts of extract, 5 parts of Quercetin, 5 parts of Yeradetajo extract, 0.1 part of antioxidant, 54.9 parts of powdered rice hulls;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Embodiment 8
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, Semen sesami nigrum 10 parts of extract, 5 parts of Quercetin, 5 parts of Yeradetajo extract, 5 parts of radix-polygoni multiflori extract, 0.1 part of antioxidant, powdered rice hulls 44.9 Part;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Embodiment 9
Increase black goldfish premix for black imperial eyeball the present embodiment provides a kind of, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, Semen sesami nigrum 10 parts of extract, 5 parts of Quercetin, 5 parts of eclipta, 5 parts of radix polygonati officinalis, 0.1 part of antioxidant, 44.9 parts of powdered rice hulls;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Comparative example 1
This comparative example provide it is a kind of increase black goldfish premix for black imperial eyeball, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 1.5 parts of trace element premix agent, 0.1 part of antioxidant, rice 97.4 parts of shell powder;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Comparative example 2
This comparative example provide it is a kind of increase black goldfish premix for black imperial eyeball, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 1.5 parts of trace element premix agent, 25 parts of tyrosine, antioxygen 0.1 part of agent, 72.4 parts of powdered rice hulls;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Comparative example 3
This comparative example provide it is a kind of increase black goldfish premix for black imperial eyeball, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 4 parts of trace element premix agent, 0.1 part of antioxidant, rice husk 94.9 parts of powder;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
Comparative example 4
This comparative example provide it is a kind of increase black goldfish premix for black imperial eyeball, by weight, be grouped as by following group:
In the goldfish premix, 1 part of vitamin premix, 1.5 parts of trace element premix agent, Semen Sesami Nigrum extract 10 Part, 0.1 part of antioxidant, 87.4 parts of powdered rice hulls;
Wherein, the component and dosage of vitamin premix and trace element premix agent are same as Example 1.
The measurement of 1 chromatic value of test example
Goldfish premix described in Examples 1 to 9 and comparative example 1~4 is used to add base by weight for the ratio of 4:100 In plinth daily ration, the identical black fine goldfish of dragon is fed, after culture experiment, 3 tail fishes are taken from every mouthful of net cage at random, with suction Water paper blots fish body surface moisture, and by colour difference meter, (WSC-S type colour difference meter, Shanghai Precision Scientific Apparatus Co., Ltd are physico Learn instrument plant) probe be close to fish body side line more than skin of back and side line skin of abdomen below, measure back respectively With skin of abdomen chromatic value, brightness (L*), red green degree (a*), champac degree (b*) value are recorded.As a result as follows:
The black imperial eyeball skin of back chromatic value of table 1
L value A value B value
Embodiment 1 25.3 3.66 5.33
Embodiment 2 22.6 4.38 3.83
Embodiment 3 23.7 2.36 2.35
Embodiment 4 22.8 6.23 4.66
Embodiment 5 17.46 1.18 -0.01
Embodiment 6 18.25 2.33 1.36
Embodiment 7 17.22 5.21 2.33
Embodiment 8 17.59 5.12 -0.21
Embodiment 9 19.23 2.31 3.28
Comparative example 1 29.3 9.12 13.17
Comparative example 2 19.92 5.18 3.01
Comparative example 3 20.15 3.44 2.21
Comparative example 4 21.13 4.14 4.72
L value: brightness;A value: red green degree;B value: champac degree
It can be seen that adding the forage feed of the goldfish premix of embodiment 5~9 in the color contrast of skin of back The black fine goldfish of dragon L value and a value be lower than comparative example 1~4, embodiment 5~9 feed the black fine goldfish of dragon b value lower than compares Example 1~4;Wherein, the effect of embodiment 7 and 8 is more significant.
The black imperial eyeball skin of abdomen chromatic value of table 2
L value: brightness;A value: red green degree;B value: champac degree
It follows that adding the black of the forage feed of the goldfish premix of embodiment 5~9 in the color contrast of abdomen The L value of the fine goldfish of dragon is substantially less than comparative example 1~4;Add the black dragon of the forage feed of the goldfish premix of embodiment 5~9 The back of fine goldfish and the color of abdomen are significant black in comparative example 1~4, and wherein the effect of embodiment 7 and 8 is the most significant.
By Fig. 1~13 it is found that the black cell volume of embodiment group becomes larger, especially embodiment 5-9 group, black cell volume increase Greatly, Dendritic arborization becomes more, compared with the control group significant effect.
The measurement of 2 skin of back tyrosinase activity of test example
The black fine goldfish of dragon that test example 1 is fed, takes 1g or so skin of back, in 1:5 ratio 67mmol/L pH 6.8 Phosphate buffer be homogenized (ice-water bath), at 4 DEG C 8000r/min be centrifuged 25min, take supernatant.Take 3mg/mL L-3,4 dihydroxyphenylalanine The above-mentioned supernatant of 2mL of 28 DEG C of preheatings, total reaction volume 2.5mL, immediately at room temperature with light splitting light after mixing is added in 0.5mL Degree meter measures its absorbance value at 475nm, measures absorbance value again after 10min.Tyrosinase activity=△ OD475/ 0.001*V*T。△OD475The difference of absorbance is measured for 2 times;V is sample volume;T is 2 measuring interval times.
The black imperial eyeball skin of back tyrosinase activity of table 3
It can be seen that the back skin of the black fine goldfish of dragon of the forage feed of the addition goldfish of embodiment 5~9 and premix Skin tyrosine enzyme activity is significantly higher than comparative example 1~4;Wherein, the effect of embodiment 7 and 8 is the most significant.
The detection of 3 scale chromatophore form of test example:
The melanocyte quantity being distributed in same size area of visual field is observed under 20 power microscopes, while right The form of chromatophore is observed.By forage feed described in embodiment 1, comparative example 1~3 and control group in test example 1 The black fine goldfish of dragon is observed.
Fig. 1 is the black fine goldfish of dragon of forage feed described in comparative example 1;
Fig. 2 is the black fine goldfish of dragon of forage feed described in comparative example 2;
Fig. 3 is the black fine goldfish of dragon of forage feed described in comparative example 3;
Fig. 4 is the black fine goldfish of dragon of forage feed described in comparative example 4;
Fig. 5 is the black fine goldfish of dragon of forage feed described in embodiment 1;
Fig. 6 is the black fine goldfish of dragon of forage feed described in embodiment 2;
Fig. 7 is the black fine goldfish of dragon of forage feed described in embodiment 3;
Fig. 8 is the black fine goldfish of dragon of forage feed described in embodiment 4;
Fig. 9 is the black fine goldfish of dragon of forage feed described in embodiment 5;
Figure 10 is the black fine goldfish of dragon of forage feed described in embodiment 6;
Figure 11 is the black fine goldfish of dragon of forage feed described in embodiment 7;
Figure 12 is the black fine goldfish of dragon of forage feed described in embodiment 8;
Figure 13 is the black fine goldfish of dragon of forage feed described in embodiment 9;
As shown, the scale of dragon-eyes body, melanocyte are evenly distributed, the black cell volume of embodiment group becomes larger, especially It is embodiment 5-9 group, and black cell volume increases, and Dendritic arborization becomes more.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art 's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed Range.

Claims (10)

1. a kind of increase black goldfish premix for black imperial eyeball, which is characterized in that premixed including tyrosine, and selected from vitamin One of agent, trace element premix agent, plant extracts pre-mixing agent or a variety of compositions.
2. goldfish premix according to claim 1, which is characterized in that premixed including 20~30 parts of tyrosine, vitamin 1~2 part of agent, 1~4 part of trace element premix agent, 10~25 parts of plant extracts pre-mixing agent.
3. goldfish premix according to claim 1 or 4, which is characterized in that the vitamin premix by weight, Including following component:
Vitamin A 50%:3~5%, vitamin D 50%:1~2%, vitamin E 50%:35~40%, vitamin B1 96%:0.2~0.4%, vitamin B2 78%:3~5%, vitamin B6 96%:3~5%, pantothenic acid 99%:11~15%, cigarette Amide 99%:11~15%, folic acid 98%:5~7%, biotin 2%:11~15%;
Preferably, vitamin A 50%:4%, vitamin D 50%:1.5%, vitamin E 50%:37.5%, vitamin B1 96%:0.3%, vitamin B2 78%:4%, vitamin B6 96%:4%, pantothenic acid 99%:13%, niacinamide 99%:13%, Folic acid 98%:6%, biotin 2%:13%.
4. described in any item goldfish premixes according to claim 1~3, which is characterized in that in the trace element premix agent Microelement be selected from one of copper, iron, zinc or a variety of;
Preferably, the trace element premix agent by weight, specifically:
The trace element premix agent by weight, specifically: ferrous sulfate 28%:45~50%, zinc sulfate 34%:35~ 37%, manganese sulfate 30%:3~3.5%, copper sulphate 25%:8~9%, calcium iodate 10%:1.2~1.7%, sodium selenite 1%: 3~3.4%;
It is furthermore preferred that ferrous sulfate 28%:48.2%, zinc sulfate 34%:35.4%, manganese sulfate 30%:3.2%, copper sulphate 25%:8.5%, calcium iodate 10%:1.5%, sodium selenite 1%:3.2%.
5. goldfish premix according to any one of claims 1 to 4, which is characterized in that the plant extracts pre-mixing agent In plant extracts in radix-polygoni multiflori extract, Semen Sesami Nigrum extract, Quercetin, Yeradetajo extract, Rhizoma Polygonati Odorati extract It is one or more;
Preferably, the plant extracts in the plant extracts is alcohol extract.
6. goldfish premix according to claim 5, which is characterized in that the plant extracts pre-mixing agent, by weight Meter, specifically: 8~12 parts of Semen Sesami Nigrum extract, 4~6 parts of Quercetin, 4~6 parts of Yeradetajo extract;Or, further including the fleece-flower root 4~6 parts of extract;
Preferably, 10 parts of Semen Sesami Nigrum extract, 5 parts of Quercetin, 5 parts of Yeradetajo extract;Or, further including radix-polygoni multiflori extract 5 Part.
7. described in any item goldfish premixes according to claim 1~6, which is characterized in that in the goldfish premix: junket ammonia The weight ratio of acid and vitamin premix are as follows: 25:0.8~1.2;And/or the weight ratio of tyrosine and trace element premix agent Are as follows: 25:3~5;And/or the weight ratio of tyrosine and plant extracts pre-mixing agent is 25:20~25.
8. described in any item goldfish premixes according to claim 1~7, which is characterized in that by weight, including such as the following group It is grouped as:
1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, 10 parts of Semen Sesami Nigrum extract, 5 parts of Quercetin, 5 parts of Yeradetajo extract;
Or, 1 part of vitamin premix, 4 parts of trace element premix agent, 25 parts of tyrosine, 10 parts of Semen Sesami Nigrum extract, Quercetin 5 Part, 5 parts of Yeradetajo extract, 5 parts of radix-polygoni multiflori extract.
9. described in any item goldfish premixes according to claim 1~8, which is characterized in that further include auxiliary material, the auxiliary material is It is one or two kinds of in antioxidant, powdered rice hulls.
10. a kind of feed of the black fine goldfish of dragon, which is characterized in that including basal ration and according to any one of claims 1 to 9 Goldfish premix;
Preferably, the basal diet and the amount ratio of the goldfish premix are 100:3~5;Most preferably 100:4.
CN201811185209.4A 2018-10-11 2018-10-11 It is a kind of to increase black goldfish premix for black imperial eyeball Pending CN109393200A (en)

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