CN109368107A - A kind of biological sample repository and its storage method - Google Patents
A kind of biological sample repository and its storage method Download PDFInfo
- Publication number
- CN109368107A CN109368107A CN201811330237.0A CN201811330237A CN109368107A CN 109368107 A CN109368107 A CN 109368107A CN 201811330237 A CN201811330237 A CN 201811330237A CN 109368107 A CN109368107 A CN 109368107A
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- Prior art keywords
- shovel
- repository
- cryopreservation tube
- holding vessel
- promotion
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- 239000012472 biological sample Substances 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000002631 hypothermal effect Effects 0.000 claims abstract description 123
- 238000005138 cryopreservation Methods 0.000 claims abstract description 106
- 238000012546 transfer Methods 0.000 claims abstract description 64
- 230000005540 biological transmission Effects 0.000 claims abstract description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 73
- 229910052757 nitrogen Inorganic materials 0.000 claims description 37
- 230000007704 transition Effects 0.000 claims description 37
- 230000007246 mechanism Effects 0.000 claims description 19
- 230000033001 locomotion Effects 0.000 claims description 18
- 230000001737 promoting effect Effects 0.000 claims description 13
- 238000012423 maintenance Methods 0.000 claims description 12
- 238000007791 dehumidification Methods 0.000 claims description 7
- 238000000151 deposition Methods 0.000 claims description 5
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 2
- 230000000630 rising effect Effects 0.000 claims 1
- 230000032258 transport Effects 0.000 abstract description 15
- 230000006378 damage Effects 0.000 abstract description 3
- 206010021113 Hypothermia Diseases 0.000 description 101
- 238000010586 diagram Methods 0.000 description 11
- 230000008569 process Effects 0.000 description 8
- 238000003032 molecular docking Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 238000009434 installation Methods 0.000 description 3
- 230000001174 ascending effect Effects 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- UPLPHRJJTCUQAY-WIRWPRASSA-N 2,3-thioepoxy madol Chemical compound C([C@@H]1CC2)[C@@H]3S[C@@H]3C[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@](C)(O)[C@@]2(C)CC1 UPLPHRJJTCUQAY-WIRWPRASSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G1/00—Storing articles, individually or in orderly arrangement, in warehouses or magazines
- B65G1/02—Storage devices
- B65G1/04—Storage devices mechanical
Abstract
The invention discloses a kind of biological sample repositories, including repository cabinet, multiple profound hypothermia holding vessels and automatic transshipment acitivity；At least one described profound hypothermia holding vessel and automatic transshipment acitivity are arranged in the repository cabinet；Pass-through box is offered on the repository cabinet, the automatic transshipment acitivity can receive the transfer tank for having cryopreservation tube come from outside by pass-through box transmission；And the cryopreservation tube in the transfer tank received can be transferred in the profound hypothermia holding vessel by the automatic transshipment acitivity；It solves the problem of that biological sample repository in the prior art not can guarantee full cold chain protection and improve destruction risk to the storage of biological sample, it is very inconvenient to solve the problems, such as that profound hypothermia holding vessel in the prior art transports；The invention also provides a kind of storage methods of biological sample repository.
Description
Technical field
The present invention relates to biological sample storage equipment area more particularly to a kind of biological sample repository and its storage sides
Method.
Background technique
Biological sample bank is that standardization collects, processing, stores health and the large biological molecule of disease organism, cell, group
Knit the equipment with organ equal samples.Before storing, need to cool down by program makes its suspend mode to biological sample, is then stored in liquid nitrogen and holds
In device or profound hypothermia refrigerator.For certain specific biological samples, storing process needs to guarantee full cold chain protection, and whole process all needs
, to protect its bioactivity, the damage of multigelation is avoided in profound hypothermia environment.
Existing repository is to set to put multiple profound hypothermia holding vessels in a fixed warehouse, needs to store up cryopreservation tube
It when depositing or transferring, is manually entered in warehouse, the profound hypothermia holding vessel for finding corresponding classification storage is manually stored；To life
When object sample is stored, it is manually entered warehouse, opening profound hypothermia holding vessel, the cryopreservation tube from transfer tank is put by cryopreservation tube
In profound hypothermia holding vessel；It needs exist for further illustrating, biological sample puts it into and freezes when most starting to be extracted
In pipe, transfer transport, cryopreservation tube is put into transfer tank for convenience.Storage of the prior art to cryopreservation tube, is to pass through people
Work operation, have a more limitation: 1. it is artificial during storage so that the process cryopreservation tube or transfer tank of storage are located always
In non-cryogenic environment, biggish destructive risk is brought to biological sample；2, artificial storage enters warehouse, needs first to find
The profound hypothermia holding vessel of the affiliated classification of biological sample, is then stored into, low efficiency again, and high labor cost is not easy
Classified storage, risk are big；3. manually when opening the storage of profound hypothermia holding vessel progress cryopreservation tube, profound hypothermia holding vessel opening
Size has to comply with the size of the operational readiness of people, this process, which opens influence of the profound hypothermia holding vessel to itself temperature, destruction
Property, by taking profound hypothermia holding vessel is liquid nitrogen container as an example, operation nitrogen consuming amount can be very big in this way；4. artificial storage is also easy to produce sample and transports mutually
Influence and pollution mutually, risk between dynamic is high；5. existing storehouse type set-up mode, it has not been convenient to install, transport, live adaptability
Difference；6. the subsequent maintenance to profound hypothermia storage warehouse, very inconvenient.
This mode not can guarantee cryopreservation tube and guarantee the protection of full cold chain storing or transfer in whole process；Not only to freezing
Biological sample in pipe generates very havoc risk, existing when requiring high to the protection of full cold chain to certain specific biological samples
There is this storing mode of technology to appear to be worthless；
When needing to biological sample classification global transfer, it is also very inconvenient；Profound hypothermia holding vessel volume itself is larger,
It is also very inconvenient when being configured transport to profound hypothermia holding vessel；
Prior art storing mode is bad in a word, and access pipe is more inconvenient, makes troubles for user.
Summary of the invention
It is an object of the present invention to provide a kind of biological sample repositories, solve biological sample storage in the prior art
Library not can guarantee the problem of full cold chain protection, improves the problem of destroying risk to the storage of biological sample, solves in the prior art
Profound hypothermia holding vessel transport very inconvenient problem.
It is a further object to provide the storage methods of biological sample repository, solve storage in the prior art
Mode is inconvenient, it cannot be guaranteed that whole cold chain protection, efficiency of storage is low, transports inconvenient problem.
The present invention solves first technical problem and adopts the following technical scheme that a kind of biological sample repository, including storage
Library cabinet, multiple profound hypothermia holding vessels and automatic transshipment acitivity；At least one described profound hypothermia holding vessel and automatic transshipment acitivity
It is arranged in the repository cabinet；Pass-through box is offered on the repository cabinet, the automatic transshipment acitivity can receive
The transfer tank for having cryopreservation tube come from outside by pass-through box transmission；And the transfer that the automatic transshipment acitivity can will receive
Cryopreservation tube in tank is transferred in the profound hypothermia holding vessel.
Preferably, it is provided with the hand basket of moveable position in the profound hypothermia holding vessel, is provided in the hand basket multiple
Tray support, bracketing has cryopreservation tube pallet in the tray support；The profound hypothermia holding vessel is provided with storage door, each
The tray support can be with the movement of hand basket, to be moved to position corresponding with the storage door；It is described automatic
Transshipment acitivity include shovel sending component, the shovel sending component may pass through storage door by the cryopreservation tube pallet in hand basket scoop up receive or can
Received cryopreservation tube pallet shovel is sent back in hand basket.
Preferably, the shovel sending component includes that shovel send box body, shovel to send the first actuator, shovel that the second actuator, shovel is sent to send cunning
Dynamic pedestal, slide-and-guide plate, shovel disk component and pallet；The shovel, which is sent, to be offered shovel and send delivery port on box body, and the shovel is sent
Delivery port can be corresponding with the storage door of the profound hypothermia holding vessel；The pallet and the shovel send sliding bottom to be arranged at
The shovel is sent in box body, and the slide-and-guide plate is slidably disposed in the shovel and send on sliding bottom, and the shovel disk component can
The setting of sliding is on the slide-and-guide plate；The pallet can keep in transfer tank；The shovel is sent to be opened up on box body
There is shovel to send logical nitrogen hole, the shovel send logical nitrogen hole to be connected to nitrogen supply system；The shovel send the first actuator to the slide-and-guide plate
Sliding driven, it is described shovel send the second actuator to shovel disk component sliding drive.
Preferably, it includes left sliding seat, right sliding seat and bedframe that the shovel, which send sliding bottom, the left sliding seat with
Right sliding seat is separately positioned on the left and right sides of the bedframe；
The slide-and-guide plate includes left slide-and-guide track, right slide-and-guide track and sliding panel；The left sliding is led
The left and right sides end of the sliding panel is separately positioned on to track and right slide-and-guide track；
The shovel disk component includes shoveling sliding part of facing left, the right sliding part of shovel disk and shovel disk；It is described to shovel face left sliding part and shovel
The left and right sides of the shovel disk is arranged in the right sliding part of disk；
The outside of the left slide-and-guide track is slided on the left sliding seat, the outside of the right slide-and-guide track
It is slided on the right sliding seat, the sliding part of facing left that shovels slides on the inside of the left slide-and-guide track, the shovel
The upper sliding on the inside of the right slide-and-guide track of the right sliding part of disk.
Preferably, the left sliding seat includes sliding support plate and multiple pulleys, and the multiple pulley setting in a row exists
On the sliding support plate；The right sliding seat and the left sliding holder structure are identical and arranged symmetrically；
The left slide-and-guide track is at I-shaped, the right slide-and-guide track and the left slide-and-guide track knot
Structure is identical and arranged symmetrically；
It includes that shovel send driving motor and lead screw that the shovel, which send the first actuator, is provided with lead screw on the sliding panel lower end surface
Nut, the feed screw nut are connect with threads of lead screw, and the shovel send the motor shaft of driving motor to connect with the lead screw.
Preferably, the automatic transshipment acitivity further includes barcode scanning component, and the barcode scanning component can be to the cryopreservation tube that it is transported
Carry out barcode scanning.
Preferably, the automatic transshipment acitivity further includes that transhipment receives cabinet and manipulator；
The transhipment receives cabinet and the shovel send box body to be arranged side by side, the manipulator setting in the shovel sending component and
The transhipment receives the top of cabinet；
The transhipment receives cabinet and receives the transfer tank come in from pass-through box conveying；The manipulator can be to transfer tank
It is grabbed；
The shovel, which is sent, to be offered shovel and send receiving port on cassette upper end face, the manipulator, which can be moved into, send receiving port phase with shovel
Corresponding position.
Preferably, the automatic transshipment acitivity further includes transhipment shell；The manipulator includes crawl driving assembly, Z axis
Set, Z axis bar, Y-axis guide rail, the first X-axis guide rail, the second X-axis guide rail, Zhua Guan mechanism and take pipe fitting；
First X-axis guide rail and the distribution of the second X-axis guide rail are fixed on the two side ends of the transhipment shell, and the Y-axis is led
Slidably it is arranged in first X-axis guide rail and the second X-axis guide rail to the two side ends of rail；The Z axis set is vertically arranged, and Z
It is slidably disposed in the middle part of axle sleeve on the Y-axis guided way；
The Z axis bar is slidably disposed in the Z axis set, and the lower end of the Z axis bar is provided with Z axis supporting, institute
Shu Zhua tank mechanism and pipe fitting is taken to be separately positioned on institute's Z axis supporting；
The Zhua Guan mechanism can grab transfer tank, described to take pipe fitting that carry out taking pipe to cryopreservation tube；
The crawl driving assembly respectively drives the movement of the Z axis set, Y-axis guide rail and Z axis bar.
Preferably, swivel mount, rotary drive assembly and lifting member are provided in the profound hypothermia holding vessel；
The lifting member is nested in the swivel mount, and the top of the lifting member and the profound hypothermia holding vessel
Tank body mutually fix；The rotary drive assembly drives swivel mount, and swivel mount may make to enclose in profound hypothermia holding vessel
It is rotated around the lifting member；
The swivel mount is divided into multiple storage areas by multiple reticles, and it is logical that promotion operation is offered on the storage area
Road, each promotion operating walk way connect with the lifting member；
Hand basket is provided in the storage area；The hand basket periphery setting one that extends outwardly pushes and receives block, and described pushes away
Dynamic reception block passes through the promotion operating walk way and extends to position corresponding with the lifting member；
The lifting member can be by promoting operating walk way in storage area to the effect driving hand basket edge for pushing reception block
It moves up and down.
Preferably, the swivel mount upper center is recessed with downwards transition axial groove, the periphery of the transition axial groove to
Outer extension is provided with multiple demarcation plates, and multiple promotion operating walk ways are offered on the circumferential cell wall of the transition axial groove；Institute
It states and promotes the storage area one-to-one correspondence that operating walk way and demarcation plate are divided into；
The lifting member includes promoting sleeve, promoting driving motor, promote driving wheel, promote driven wheel, promote transition
Wheel and lifter board；
The promotion sleeve is arranged in the transition axial groove, and can be with swivel mount relative rotation；The promotion sleeve
Top circumference be outward extended with promotion transition plates, the promotion driving motor is arranged outside the profound hypothermia holding vessel, and
The motor shaft for promoting driving motor extends downwardly the cover for sequentially passing through profound hypothermia holding vessel and promotes transition plates and is provided with
Promote driving wheel；
The promotion sleeve periphery upper end is provided with promotion transition wheel and engages connection, the promotion with the promotion driving wheel
Sleeve periphery lower end is provided with promotion driven wheel, and promotion transmission is provided between the promotion driven wheel and the promotion transition wheel
Band；Lifter board is provided on the promotion transmission belt, the lifter board is corresponding with promotion reception block.
Preferably, be provided on the demarcation plate storage guiding dragging track, the hand basket periphery be also equipped with it is described
Storage guiding hand basket draw runner of the dragging track to cooperation, the hand basket draw runner slide on storage guiding dragging track.
Preferably, described to promote the pipe sleeve that sleeve is inner hollow；
Turning effort axis, turning effort axis are equipped with to promotion sleeve internal stretch at the slot bottom center of the transition axial groove
Upper end is provided with " ten " word receiving slit；
The rotary drive assembly includes rotary drive motor and rotation drive shaft, and the rotary drive motor is fixed on institute
It states outside profound hypothermia holding vessel, and the motor shaft of the rotary drive motor and the rotation drive shaft are coaxially connected, the rotation
Turn drive shaft lower end and be provided with " ten " word shackle member, and the shackle member for rotating drive shaft is fastened in receiving slit.
Preferably, the hand basket is at sector, and the two sides in the hand basket are provided with multiple support bars, and every two same
Support bar on horizontal plane forms the tray support.
Preferably, the cryopreservation tube pallet is the sector mutually agreed with hand basket, and is provided on the cryopreservation tube pallet more
A to deposit tube seat, the slot bottom for depositing tube seat is exploitation formula slot bottom.
It preferably, further include maintenance door, nitrogen supply system and dehumidification system, the maintenance door is arranged in the repository cabinet
On, repository cabinet can be opened by maintenance door, its inside is overhauled；
The nitrogen supply system can to being carried out in repository cabinet for nitrogen, can also in automatic transshipment acitivity for nitrogen；
The dehumidification system can dehumidify to repository cabinet.
The present invention solves the storage side that another technical problem adopts the following technical scheme that a kind of biological sample repository
The pass-through box that the transfer tank for having cryopreservation tube directly passes through repository cabinet is put into repository by method, R step；S step,
Cryopreservation tube in the transfer tank received is transferred in the profound hypothermia holding vessel by the automatic transshipment acitivity in repository automatically.
Preferably, the received transfer tank of repository is grabbed the temporary of setting by the manipulator of S1 step, automatic transshipment acitivity
It deposits on frame；
S2 step, while carrying out S1 step or after above-mentioned R step, automatic transshipment acitivity can be along given line
It moves at corresponding profound hypothermia holding vessel；
The storage door of S3 step, profound hypothermia holding vessel is opened, and the shovel sending component of automatic transshipment acitivity is to profound hypothermia holding vessel
Interior cryopreservation tube pallet shovels on the corresponding platform of automatic transshipment acitivity；
S4 step, the cryopreservation tube that manipulator grabs on pallet in transfer tank are placed on cryopreservation tube pallet；
Cryopreservation tube tray is transmitted back in profound hypothermia holding vessel by S5 step, shovel sending component again.
Preferably, when the S4 step carries out, manipulator first grabs cryopreservation tube to barcode scanning component from pallet
Side carries out barcode scanning to the two dimensional code on cryopreservation tube, and the control system of setting is handled and stored to barcode scanning result, again will later
Cryopreservation tube is grabbed to cryopreservation tube pallet.
Preferably, T step, before S3 step progress, the corresponding hand basket in profound hypothermia holding vessel is moved to
Position corresponding with storage door；
The T step is divided into:
T1 step, the rotary drive assembly driving swivel mount rotation of profound hypothermia holding vessel, to drive mentioning on swivel mount
Basket is moved in the circumferential at the corresponding position of storage door of profound hypothermia holding vessel；
T2 step, lifting member promote hand basket, may pass through cryopreservation tube tray lift to the shovel sending component on its hand basket
Storage door shovels the position sent to it.
Preferably, further include G step: repository cabinet is directly directly transported by being equipped with the transport vehicle of container.
The present invention have following first the utility model has the advantages that
1, biological sample repository facilitates whole Transporting, easy for installation, and scene is adaptable, and extendibility is strong；
2, storage is reliable for operation high-efficient, and storage capacity is big；
3, storage is convenient, easily classifies；
4, energy saving, effectively reduce the energy (liquid nitrogen) consumption；
5, can reduce sample mutually move between influence and risk of cross-contamination；
6, maintenance is convenient, just in case its is faulty Ke Kuaisugenghuan.
The present invention have following second the utility model has the advantages that can fast storage, guarantee whole cold chain storage, fast and accurately divide
Class storage, it is high-efficient, greatly reduce the storage method of cost of labor.
Detailed description of the invention
Fig. 1 is biological sample repository structural schematic diagram；
Fig. 2 is biological sample repository structural decomposition diagram；
Fig. 3 is biological sample repository top view；
Fig. 4 is profound hypothermia holding vessel schematic diagram of internal structure；
Fig. 5 is profound hypothermia holding vessel cross-sectional view；
Fig. 6 is swivel mount and lifting member structural schematic diagram；
Fig. 7 is structural schematic diagram of the hand basket from rearview direction；
Fig. 8 is structural schematic diagram of the hand basket from preceding view direction；
Fig. 9 is lifting member structural schematic diagram；
Figure 10 is rotary drive assembly and promotion driving motor structural schematic diagram；
Figure 11 is automatic transshipment acitivity structural schematic diagram；
Figure 12 is automatic transshipment acitivity schematic diagram of internal structure；
Figure 13 is shovel sending component and robot manipulator structure schematic diagram；
Figure 14 is shovel sending component structural exploded view.
The mark in the figure shows:
1- repository cabinet；101- pass-through box；
2- profound hypothermia holding vessel；201- hand basket；202- tray support；203- cryopreservation tube pallet；204- storage door；205-
Swivel mount；The storage area 206-；207- lifting member；208- rotary drive assembly；209- promotes operating walk way；2010- promotion connects
Receive block；2011- transition axial groove；2012- demarcation plate；2013- promotes sleeve；2014- promotes driving motor；2015- promotes master
Driving wheel；2016- promotes driven wheel；2017- promotes transition wheel；2018- lifter board；2019- promotes transition plates；2020-, which is promoted, to be passed
Send band；2022- storage guiding dragging track；2023- hand basket draw runner；2024- turning effort axis；2025- receiving slit；2026- rotation
Turn driving motor；2027- rotates drive shaft；2028- shackle member；2029- support bar；2030- deposits tube seat；
The automatic transshipment acitivity of 3-；
301- shovels sending component；3011- shovel send box body；3012- shovel send the first actuator；3014- shovel send sliding bottom；
3015- slide-and-guide plate；3016- shovels disk component；3017- pallet；3018- shovel send delivery port；3019- shovel send logical nitrogen hole；
The left sliding seat of 30110-；The right sliding seat of 30111-；30112- bedframe；The left slide-and-guide track of 30113-；The right cunning of 30114-
Action-oriented track；30115- sliding panel；30116- shovels sliding part of facing left；30117- shovels the right sliding part of disk；30118- shovels disk；
30119- sliding support plate；30120- pulley；30121- shovel send driving motor；30122- lead screw；30123- feed screw nut；
30124- shovel send receiving port；
302- barcode scanning component；3021- scanner；3022- barcode scanning mirror surface；3023- code reader mounting groove；
303- transhipment receives cabinet；3031- first is open；3032- second is open；3033- transports upper cover；
304- manipulator；3041- grabs driving assembly；3042-Z axle sleeve；3043-Z shaft；3044-Y axis rail；3045-
First X-axis guide rail；The second X-axis guide rail of 3046-；3047- Zhua Guan mechanism；3048- takes pipe fitting；3049-Z bearing fitting；
305- transports shell；
4- transfer tank；5- maintenance door；6- nitrogen supply system；7- dehumidifier；8- transports track automatically.
Specific embodiment
Technical solution of the present invention is further elaborated below with reference to examples and drawings.
Embodiment 1
Present embodiments provide kind of a biological sample repository, including repository cabinet 1, multiple profound hypothermia holding vessels 2 and from
Dynamic transshipment acitivity 3；At least one described profound hypothermia holding vessel 1 and automatic transshipment acitivity 3 are arranged at the repository cabinet 1
It is interior；Pass-through box 101 is offered on the repository cabinet 1, the automatic transshipment acitivity 3 can receive from outside by pass-through box
The transfer tank 4 for having cryopreservation tube that 101 transmission come；And the automatic transshipment acitivity 3 can be by freezing in the transfer tank 4 received
Pipe is transferred in the profound hypothermia holding vessel 2.
One repository cabinet 1 is set, multiple profound hypothermia holding vessels 2 are set in repository cabinet 1, in multiple profound hypothermias
Automatic operating mechanism 3 is set between holding vessel 2, and automatic operating mechanism 3 can not only move between multiple profound hypothermia holding vessels 2
It is dynamic, and automatic transshipment acitivity 3 can be corresponding with the pass-through box 101 of repository cabinet 1, inside has the transfer tank 4 of cryopreservation tube to pass through
Pass-through box 101 enters automatic transshipment acitivity 3, and automatic transshipment acitivity 3 carries transfer tank 101 and moves to corresponding profound hypothermia storage
At tank 2, the cryopreservation tube of transfer tank 101 is transferred in depth cryogenic storage tank 2 and is stored；Certainly it is here provided with control
System controls the running of profound hypothermia holding vessel 2 and automatic transshipment acitivity 3；Nitrogen supply system is arranged not only to store profound hypothermia
Tank 2 carries out keeping low-temperature condition for nitrogen, can also be continued to make it be in profound hypothermia state for nitrogen to automatic operating mechanism 3.
Biological sample repository of the invention, 1, it is this by multiple profound hypothermia holding vessels be arranged in repository cabinet, side
Just live installation, adaptable, extendibility is strong；Profound hypothermia holding vessel in repository cabinet can be needed to be arranged according to scene
Number, position etc.；When transporting to it, also convenient transportation, is directly transported using storage box body；2. changing existing
There is bring destructiveness risk during manually storing in technology, so that transfer tank or cryopreservation tube are constantly in low temperature environment,
Greatly reduce the destructive risk during storage；3, it does not need to be manually entered warehouse, directly have in the reception of repository cabinet
Turn tank and transfer tank is transported through by automatic transshipment acitivity, corresponding profound hypothermia holding vessel is docked by transfer tank, it can be fast
The correspondence classified storage of speed, and it is accurate, it is high-efficient, greatly reduce risk；4, automatic transshipment acitivity is stored with profound hypothermia again
When tank docking storage, the mouth for the corresponding size of transfer tank that can be stored can be only opened, and storage is rapid, in place, avoids
Manually when opening profound hypothermia holding vessel and being stored, wind that profound hypothermia holding vessel inner cryogenic storage condition can be destroyed
Danger, greatly reduces nitrogen consuming amount, stability is strong；5, the risk for reducing the influence between sample interactive movement and polluting mutually；6, after
It is continuous very convenient to its maintenance.
The present embodiment further discloses that a kind of automatic transshipment acitivity 3 docks conveying with profound hypothermia holding vessel 2 and storage is frozen
Deposit the frame mode of pipe；The hand basket 201 of moveable position, setting in the hand basket 201 are provided in the profound hypothermia holding vessel 2
There are multiple tray supports 202, bracketing has cryopreservation tube pallet 203 in the tray support 202；The profound hypothermia holding vessel 2
It is provided with storage door 204, each tray support 202 can be with the movement of hand basket 201, to be moved to and the storage
Deposit the corresponding position of door 204；The automatic transshipment acitivity 3 includes shovel sending component 301, and the shovel sending component 301 may pass through storage
Door 204 is deposited the cryopreservation tube pallet 203 in hand basket 201 is scooped up reception or the reception shovel of cryopreservation tube pallet 203 can be sent back to hand basket 201
It is interior.In order to guarantee stability that automatic transshipment acitivity 3 transports cryopreservation tube, also for reduce profound hypothermia holding vessel 2 with it is automatic
The storage door 204 opened is as small as possible when transshipment acitivity 3 docks, and ensure that profound hypothermia environment inside profound hypothermia holding vessel 2 not
It is affected, so being provided with the shovel sending component 301 cooperated with profound hypothermia holding vessel 2, what is be arranged in profound hypothermia holding vessel 2 can
Multiple tray supports 202 are arranged in mobile hand basket 201, hand basket 201, and bracketing has cryopreservation tube pallet 203 in tray support 202,
In this way shovel sending component 301 pass through storage door 204, into hand basket 201 in corresponding cryopreservation tube pallet 203 shoveled out, shovel arrives
In automatic transshipment acitivity 3, then the cryopreservation tube in the transfer tank 4 received is put into freezes conduit saddle again by automatic transshipment acitivity 3
On disk 203, then the cryopreservation tube pallet 203 for having cryopreservation tube is shoveled and is sent back in profound hypothermia holding vessel 2；It not only ensure that cryopreservation tube is transported
Stability, also reducing as far as possible during transportation influences the environment of cryogenic storage tank 2.Certainly, automatic conveyance
The docking mode of structure 3 and profound hypothermia holding vessel 2 is also possible to draw or grab by manipulator, or hand basket is directly moved out depth
Cryogenic storage tank 2 is docked etc. with automatic transshipment acitivity 3, these modes cannot all provide stable effective transmission mode, i.e.,
Transmission is set to reach transmission purpose, the mode that sending component is also preferably shoveled without the present embodiment is more stable, and it is more efficient, also to environment
Do not influence.As shown in figure 3,4 profound hypothermia holding vessels 2 are arranged in repository cabinet 1, between 4 profound hypothermia holding vessels 2
The automatic transhipment track 8 of setting, the automatic transshipment acitivity 3 move on the automatic transhipment track 8, reach in 4 profound hypothermias
The purpose moved between holding vessel 2.
The present embodiment further discloses a kind of specific embodiment for shoveling sending component 301, and the shovel sending component 301 includes
Shovel send box body 3011, shovel to send the first actuator 3012, shovel that the second actuator, shovel is sent to send sliding bottom 3014, slide-and-guide plate
3015, disk component 3016 and pallet 3017 are shoveled；The shovel, which is sent, to be offered shovel and send delivery port 3018, and the shovel on box body 3011
Send delivery port 3018 corresponding with the storage door 204 of the profound hypothermia holding vessel 2；The pallet 3017 and the shovel send cunning
Dynamic pedestal 3014 is arranged at the shovel and send in box body 3011, and the slide-and-guide plate 3015 is slidably disposed in the shovel and send
On sliding bottom 3014, the shovel disk component 3016 is slidably disposed on the slide-and-guide plate 3015；The pallet
3017 can keep in transfer tank 4；The shovel, which is sent, to be offered shovel and send logical nitrogen hole 3019 on box body 3011, the shovel send logical nitrogen hole
3019 are connected to nitrogen supply system；The shovel send the first actuator 3012 to drive the sliding of the slide-and-guide plate 3015,
The shovel send the second actuator to drive the sliding of shovel disk component 3016.
Transfer tank 4 received from the pass-through box 101 of repository cabinet 1 is first first temporarily stored in temporary by automatic transshipment acitivity 3
In frame 3017, setting shovel send box body 3011, and shovel, which is sent, to be offered shovel and send logical nitrogen hole 3019 on box body 3011, the shovel send logical nitrogen hole
3019 are connected to nitrogen supply system, this ensure that shovel is sent in box body 3011 constantly by for nitrogen, it are made to be constantly in profound hypothermia shape
State, the transfer tank 4 received have also been in profound hypothermia state at once；When transfer tank 4 is temporarily stored in the process in pallet 3017
In, automatic transshipment acitivity 3 has moved to position corresponding with profound hypothermia holding vessel 2, and shovel send delivery port 3018 and profound hypothermia
The storage door 204 of holding vessel 2 is corresponding；The shovel send the first actuator 3012 driving slide-and-guide plate 3015 to send conveying to shovel
The movement of mouthful 3018 directions, rear shovel send the second actuator 3013 driving shovel disk component 3016 to move out shovel delivery port 3018 is sent to protrude into
Corresponding cryopreservation tube pallet 203 is shoveled out into profound hypothermia holding vessel 2, then is retracted into initial position；Cryopreservation tube pallet 203 is by band
Enter to after automatic transshipment acitivity, then the cryopreservation tube in transfer tank 4 is put into cryopreservation tube pallet 204；Receive cryopreservation tube it
Cryopreservation tube pallet 203 afterwards is sent back in profound hypothermia holding vessel 2 by the shovel shovel of sending component 301 again, is stored；Certainly deep low here
The door-opening mode of the storage door 204 of warm holding vessel 2 is arranged to slide up and down open mode, more easily cooperation and automatic transhipment
The docking of mechanism 3.
Setting 1. is in whole process in profound hypothermia environment in this way, substantially increases the safety of biological sample；2.
Pallet provides temporary position to transfer tank, facilitates the cryopreservation tube extracted in transfer tank；Journey is passed through in shovel 3. shoveling sending component
Middle stability is high.
Guarantee shovel pass through journey it is stable while, in order to reduce as far as possible the structure of shovel sending component 301 occupy it is empty
Between, so that it is compact-sized, it saves for the nitrogen energy, the present embodiment further preferably discloses the further implementation of shovel sending component 301
Mode, the shovel send sliding bottom 3014 to include left sliding seat 30110, right sliding seat 30111 and bedframe 30112, described
Left sliding seat 30110 is separately positioned on the left and right sides of the bedframe 30112 with right sliding seat 30111；The sliding is led
It include left slide-and-guide track 30113, right slide-and-guide track 30114 and sliding panel 30115 to plate 3015；The left sliding is led
The left and right sides end of the sliding panel 30115 is separately positioned on to track 30113 and right slide-and-guide track 30114；The shovel
Disk component 3016 includes shoveling sliding part 30116 of facing left, the right sliding part 30117 of shovel disk and shovel disk 30118；It is described to shovel sliding part of facing left
30116 and shovel the right sliding part 30117 of disk be arranged at it is described shovel disk 30118 the left and right sides；The left slide-and-guide track
30113 outside is slided on the left sliding seat 30110, and the outside of the right slide-and-guide track 30114 is in the right cunning
It is slided on dynamic seat 30111, the sliding part 30116 of facing left that shovels slides on the inside of the left slide-and-guide track 30113, institute
State the upper sliding on the inside of the right slide-and-guide track 30114 of the shovel right sliding part 30117 of disk.
The left sliding seat 30110 includes sliding support plate 30119 and multiple pulleys 30120, the multiple pulley 30120
It is in a row to be arranged on the sliding support plate 30119；The right sliding seat 30111 and the left 30110 structure phase of sliding seat
It is same and arranged symmetrically；The left slide-and-guide track 30113 at I-shaped, the right slide-and-guide track 30114 with it is described
Left 30113 structure of slide-and-guide track is identical and arranged symmetrically；It includes that shovel send driving motor that the shovel, which send the first actuator 3012,
30121 and lead screw 30122, feed screw nut 30123, the feed screw nut 30123 are provided on 30115 lower end surface of sliding panel
It is threadedly coupled with lead screw 30122, the shovel send the motor shaft of driving motor 30121 to connect with the lead screw 30122.
The shovel send the shovel of the first actuator 3012 that driving motor driving lead screw 30122 is sent to rotate, lead screw 30122 and lead screw
Nut 30123 is threadedly coupled, and rotary motion is converted into linear motion, and sliding panel 30115 is driven to move along a straight line；The left sliding is led
To track 30113 at I-shaped structure, be arranged so left slide-and-guide track 30113 a side and left sliding seat 30110
Multiple pulleys 30120 match and slided on sliding support plate 30119；Another side of I-shaped forms shovel disk simultaneously
The sliding rail of left sliding part 30116, so that shovel is faced left, sliding part 30116 is slid on；Right slide-and-guide track 30114 with
Left 30113 structure of slide-and-guide track is identical and arranged symmetrically；This set is compact-sized on space proportion, and running is steady
While determining, the Extensible of enough shovel sending components 301 is also provided.
The preferred further embodiment of the present embodiment, the automatic transshipment acitivity 3 further includes barcode scanning component 302, described
Barcode scanning component 302 can carry out barcode scanning to the cryopreservation tube that it is transported.After automatic transshipment acitivity 3 turns tank 4 in the reception, pass through pallet
3017 pairs of transfer tanks 4 are kept in, then after extracting cryopreservation tube in transfer tank 4, cryopreservation tube is first moved to barcode scanning component 302
Place carries out barcode scanning to cryopreservation tube, is then sent into profound hypothermia holding vessel 2 in shovel；In this way barcode scanning to the information of cryopreservation tube at
High standard automation is realized in reason, analysis and storage.Here barcode scanning component 302 is further disclosed to include scanner 3021 and sweep
Code mirror surface 3022, a code reader mounting groove 3023 can be arranged in automatic transshipment acitivity 3, code reader 3021 is installed to barcode scanning
In device mounting groove 3023, lid sets barcode scanning mirror surface 3022 above code reader mounting groove 3023；Facilitate barcode scanning in this way.
In order to preferably receive transfer tank 4, and transfer tank 4 is transported through and the cooperation with shovel sending component 301, this implementation
The preferred further embodiment of example, the automatic transshipment acitivity 3 further include that transhipment receives cabinet 303 and manipulator 304；It is described
Transhipment receives cabinet 303 and the shovel send box body 3011 to be arranged side by side, and the manipulator 304 is arranged in the shovel sending component 301
The top of cabinet 303 is received with the transhipment；The transhipment receives what the reception of cabinet 303 was come in from the pass-through box 101 conveying
Transfer tank 4；The manipulator 304 can grab transfer tank 4；The shovel, which send to offer shovel on 3011 upper surface of box body and send, to be connect
Close up 30124, the manipulator 304 can be moved into send receiving port 30124 corresponding position with shovel.Transhipment receives cabinet 303
On offer the first opening 3031 and the second opening 3032, first opening 3031 is bonded with pass-through box 101 to be correspondingly arranged, the
Two openings 3032 are provided with the transhipment and receive on the upper surface of cabinet 303, and transhipment upper cover can be arranged at the second opening 3032
3033；First opening 3031 receives transfer tank 4, and pass-through box 101 is closed, and the transhipment upper cover 3033 at the second opening 3032 is opened,
Manipulator 304 passes through 3032 crawl transfer tank 4 of the second opening and is put into pallet 3017, shovels sending component 301 for cryopreservation tube pallet 203
The shovel for shoveling automatic transshipment acitivity 3 is sent in box body 3011, and manipulator 304 is by the cryopreservation tube on pallet 3017 in transfer tank 4
Crawl；It first grabs to barcode scanning component, carries out barcode scanning, processing storage is carried out to the information of cryopreservation tube；Manipulator 304 is by cryopreservation tube
Crawl is moved to shovel and send at receiving port 30124, and cryopreservation tube is put into shovel and send 30124 lower section of receiving port corresponding cryopreservation tube pallet
In 203；The shovel of cryopreservation tube pallet 203 is sent back in profound hypothermia holding vessel 2 store again by shovel sending component 301.
The preferred further embodiment of the present embodiment, the automatic transshipment acitivity 3 further include transhipment shell 305；It is described
Manipulator 304 includes crawl driving assembly 3041, Z axis set 3042, Z axis bar 3043, Y-axis guide rail 3044, the first X-axis guide rail
3045, the second X-axis guide rail 3046, Zhua Guan mechanism 3047 and pipe fitting 3048 is taken；First X-axis guide rail 3045 and the second X-axis are led
Rail 3046 is distributed the two side ends for being fixed on the transhipment shell 305, and the two side ends of the Y-axis guided way 3044 are slidably arranged
In first X-axis guide rail 3045 and the second X-axis guide rail 3046；The Z axis set 3042 is vertically arranged, and in Z axis set 3042
Portion is slidably disposed on the Y-axis guided way 3044；The Z axis bar 3043 is slidably disposed in the Z axis set 3042
Interior, the lower end of the Z axis bar 3043 is provided with Z axis supporting 3049, the Zhua Guan mechanism 3047 and pipe fitting 3048 is taken to distinguish
It is arranged on institute's Z axis supporting 3049；The Zhua Guan mechanism 3047 can grab transfer tank 4, described to take pipe fitting 3048
Cryopreservation tube is carried out to take pipe；The crawl driving assembly 3041 is respectively to Z axis set 3042, Y-axis guide rail 3044 and Z axis bar
3043 movement is driven.
The two side ends of Y-axis guide rail 3044 can be slided in first X-axis guide rail 3045 and the second X-axis guide rail 3046, real
Movement in existing X-direction；Z axis set can slide in Y-axis guide rail 3044, realize the movement in Y direction；Meanwhile Z axis bar
3043 can cover the up and down motion done on vertical direction in 3042 in Z axis, realize movement in the Z-axis direction；In Z axis bar 3043
Z axis supporting 3049 is arranged in lower end, such as Figure 13, the left and right ends of Z axis supporting 3049 be respectively set Zhua Guan mechanism 3047 and
Pipe fitting 3048 is taken, the Zhua Guan mechanism 3047 can grab transfer tank 4, described to take pipe fitting 3048 that take to cryopreservation tube
Pipe；Transfer tank 4 is grabbed on pallet 3017 first with Zhua Guan mechanism 3047, the cover of transfer tank 4 is opened, can pass through here
Zhua Guan mechanism 3047 carries out crawl unlatching to cover, and can also be uncapped mode by other, just repeats no more here；Open cover
Afterwards, by taking pipe fitting, pipe fitting 3048 will be taken to grab in transfer tank 4, then carries out barcode scanning again, grabs in cryopreservation tube pallet 203
Equal subsequent actions.
It is docked to optimize storage condition and profound hypothermia holding vessel 2 as far as possible with the cooperation of automatic transshipment acitivity 3；This reality
The embodiment for applying the preferred further profound hypothermia holding vessel 2 of example, first passes through the rotation of swivel mount 205, the storage that will need to store
The hand basket 201 for depositing area 206 rotates to corresponding position, and then hand basket 201 is extracted and stored up by lifting member 207 again
Deposit the corresponding position of door 204；The design of profound hypothermia holding vessel 2 of the invention is directed in a limited space, and can sufficient benefit
With storage space, and entire hand basket 201 is not proposed, the cryopreservation tube tray 203 of needs is only subjected to operation and storage door
204 corresponding propose further optimal enforcement mode: swivel mount 205 are provided in the profound hypothermia holding vessel 2, rotation is driven
Dynamic component 208 and lifting member 207；The lifting member 207 is nested in the swivel mount 205, and the lifting member 207
Top and the tank body of the profound hypothermia holding vessel 2 mutually fix；The rotary drive assembly 208 drives swivel mount 205,
Swivel mount 205 may make to be rotated in profound hypothermia holding vessel 2 around the lifting member 207；The swivel mount more than 205
A reticle is divided into multiple storage areas 206, offers on the storage area 206 and promotes operating walk way 209, described each to mention
Lift operations channel 209 connects with the lifting member 207；Hand basket 201 is provided in the storage area 206；The hand basket
201 peripheries, which extend outwardly, is arranged a promotion reception block 2010, and the promotion receives block 2010 and passes through the promotion operating walk way
209 extend to position corresponding with the lifting member 207；The lifting member 207 can be by receiving block 2010 to promotion
Effect driving hand basket 201 along promoted operating walk way 209 moved up and down in storage area 206.Lifting member 207 is nested in rotation
In pivoted frame 205, space is greatly saved, a large amount of space can be saved and be arranged to storage area 206；Further, the rotation
205 upper center of frame is recessed with downwards transition axial groove 2011, and the periphery of the transition axial groove 2011, which extends outwardly, to be provided with
Multiple demarcation plates 2012, and multiple promotion operating walk ways 209 are offered on the circumferential cell wall of the transition axial groove 2011；It is described
The storage area 206 that operating walk way 209 and demarcation plate 2012 are divided into is promoted to correspond；The lifting member 207 includes lift lug
Cylinder 2013 promotes driving motor 2014, promotes driving wheel 2015, promote driven wheel 2016, promote transition wheel 2017 and lifter board
2018；The promotion sleeve 2013 is arranged in the transition axial groove 2011, and can be with 205 relative rotation of swivel mount；It is described
The top circumference for promoting sleeve 2013, which is outward extended with, promotes transition plates 2019, using promoting transition plates 2019 for lifting member
207 fix with 2 phase of profound hypothermia holding vessel；2 preferred embodiment of profound hypothermia holding vessel of the invention is liquid nitrogen container；The promotion
Driving motor 2014 is arranged outside the profound hypothermia holding vessel 2, and the motor shaft for promoting driving motor 2014 is to downward
It stretches the cover for sequentially passing through profound hypothermia holding vessel 2 and promotes transition plates 2019 and be provided with promotion driving wheel 2015；The lift lug
The peripheral upper end of cylinder 2013 is provided with promotion transition wheel 2017 and engages connection with the promotion driving wheel 2015, and promotion here is actively
The preferably sector gear of wheel 2015 is engaged with fan-shaped 2017 arc of promotion transition wheel, for the form setting for most saving torque；It is described
It promotes the peripheral lower end of sleeve 2013 and is provided with promotion driven wheel 2016, the promotion driven wheel 2016 and the promotion transition wheel
It is provided between 2017 and promotes conveyer belt 2020；Lifter board 2018, the lifter board are provided on the promotion transmission belt 2020
2018 is corresponding with promotion reception block 2010.
After rotary drive assembly 208 drives swivel mount 205 to rotate, i.e., each storage area 206 rotates in the axial direction, hand basket
201 rotate with storage area 206；It is provided on transition axial groove 2011, that is, is provided with because promoting operating walk way 209
On swivel mount 205,201 periphery of hand basket of each storage area 206, which extends outwardly, is arranged a promotion reception block 2010, not only guarantees
Hand basket 201 is in the stability upward with 206 revolution of storage area, moreover, when needing corresponding hand basket 201, this hand basket
201 stop being the corresponding position of lifting member 207, and certain lifting member 207 remains and storage door 204 in the circumferential
Corresponding position；It promotes the driving of driving motor 2014 and promotes driving wheel 2015, drive and promote transition wheel 2017, mentioned to drive
Conveyer belt 2020 is risen to move on promoting transition wheel 2017 and promotion driven wheel 2016；Because keeping the position in fact of lifter board 2018
Consistently lower than hand basket 201 is to the position for promoting the promotion reception block 2010 that 209 direction of operating walk way extends, so when promoting transmission
When band 2020 moves, it can be pushed upwards when moving to and pushing and receive block 2010 by promoting the lifter board 2018 on conveyer belt 2020
Movement, to drive hand basket 201 to do ascending motion along operating walk way 209 is promoted, until the cryopreservation tube pallet 203 needed is moved
Until the position for corresponding to storage door 204.
The present embodiment preferred further embodiment is provided with storage guiding dragging track on the demarcation plate 2012
2022,201 periphery of hand basket, which is also equipped with, is oriented to hand basket draw runner 2023 of the dragging track 2022 to cooperation, institute with the storage
Hand basket draw runner 2023 is stated to slide on storage guiding dragging track 2022.Enhance the stability of 201 ascending motion of hand basket.
The preferred further embodiment of the present embodiment, it is described to promote the pipe sleeve that sleeve 2013 is inner hollow；The mistake
It crosses at the slot bottom center of axial groove 2011 and is equipped with turning effort axis 2024, turning effort axis to promotion 2013 internal stretch of sleeve
2024 upper ends are provided with " ten " word receiving slit 2025；The rotary drive assembly 208 includes rotary drive motor 2026 and rotation
Drive shaft 2027, the rotary drive motor 2026 is fixed on outside the profound hypothermia holding vessel 2, and the rotary drive motor
2026 motor shaft and the rotation drive shaft 2027 are coaxially connected, and 2026 lower end of rotation drive shaft is provided with " ten " word card
Fitting 2028, and the shackle member 2028 for rotating drive shaft 2027 is fastened in receiving slit 2025.
The preferred further embodiment of the present embodiment, the hand basket 201 is at sector, and the two sides in the hand basket 201
Multiple support bars 2029 are provided with, every two support bars 2029 in same level form the tray support 202.
The preferred further embodiment of the present embodiment, the cryopreservation tube pallet 203 are the fan agreed with 201 phase of hand basket
Shape, and be provided on the cryopreservation tube pallet 203 it is multiple deposit tube seat 2030, the slot bottom for depositing tube seat 2030 is exploitation formula slot
Bottom.Open trough bottom described here, as shown in fig. 6, opening up open bore in slot bottom, in cryopreservation tube storage, cryopreservation tube can be inserted
Enter to wherein, it is more stable.
The preferred further embodiment of the present embodiment further includes maintenance door 5, nitrogen supply system and dehumidification system, the inspection
It repairs door 5 to be arranged on the repository cabinet 1, repository cabinet 1 can be opened by maintenance door 5, its inside is overhauled；Institute
State nitrogen supply system can to being carried out in repository cabinet 1 for nitrogen, can also in automatic transshipment acitivity for nitrogen；The dehumidification system can be right
Repository cabinet 1 dehumidifies, and dehumidification system includes multiple dehumidifiers 7.Maintenance is convenient, just in case its it is faulty can quickly more
It changes.
Embodiment 2
A kind of storage method of biological sample repository is present embodiments provided, R step will have the transfer tank of cryopreservation tube
Directly it is put into repository by the pass-through box of repository cabinet；S step, the automatic transshipment acitivity in repository will receive
Transfer tank in cryopreservation tube be transferred in the profound hypothermia holding vessel automatically.This storing mode has abandoned people in the prior art
Work stores bring to the risk of cryopreservation tube environmental disruption, and conveniently, efficiently, accurately.
The preferred further embodiment of the present embodiment, the manipulator of S1 step, automatic transshipment acitivity receive repository
Transfer tank grab on the pallet of setting；
S2 step, while carrying out S1 step or after above-mentioned R step, automatic transshipment acitivity can be along given line
It moves at corresponding profound hypothermia holding vessel；
The storage door of S3 step, profound hypothermia holding vessel is opened, and the shovel sending component of automatic transshipment acitivity is to profound hypothermia holding vessel
Interior cryopreservation tube pallet shovels on the corresponding platform of automatic transshipment acitivity；
S4 step, the cryopreservation tube that manipulator grabs on pallet in transfer tank are placed on cryopreservation tube pallet；
Cryopreservation tube tray is transmitted back in profound hypothermia holding vessel by S5 step, shovel sending component again.
By automatic transshipment acitivity can be movably walking between multiple profound hypothermia holding vessels in the way of, instead of existing skill
The process of docking manually is found in multiple profound hypothermia holding vessels in art, and the more convenient classification of profound hypothermia holding vessel is set
It sets, substantially increases efficiency of storage.By shovel send in the way of carry out docking between automatic transshipment acitivity and profound hypothermia holding vessel,
It substantially increases the efficiency of cryopreservation tube deposit profound hypothermia holding vessel.
The preferred further embodiment of the present embodiment, when the S4 step carries out, manipulator first will from pallet
Cryopreservation tube grabs to barcode scanning component top and carries out barcode scanning, the control system of setting, to barcode scanning result to the two dimensional code on cryopreservation tube
It is handled and is stored, later again grabbed cryopreservation tube to cryopreservation tube pallet.
The preferred further embodiment of the present embodiment, T step, before S3 step progress, profound hypothermia holding vessel
Interior corresponding hand basket is moved to position corresponding with storage door；
The T step is divided into:
T1 step, the rotary drive assembly driving swivel mount rotation of profound hypothermia holding vessel, to drive mentioning on swivel mount
Basket is moved in the circumferential at the corresponding position of storage door of profound hypothermia holding vessel；
T2 step, lifting member promote hand basket, may pass through cryopreservation tube tray lift to the shovel sending component on its hand basket
Storage door shovels the position sent to it.
The preferred further embodiment of the present embodiment, further includes G step: by repository cabinet directly by being equipped with packaging
The transport vehicle of case directly transports.One container can transport it, and scene adaptability, extendibility easy for installation are strong.
The sequencing of above embodiments is not only for ease of description, represent the advantages or disadvantages of the embodiments.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (20)
1. a kind of biological sample repository, which is characterized in that including repository cabinet, multiple profound hypothermia holding vessels and automatic transhipment
Mechanism；At least one described profound hypothermia holding vessel and automatic transshipment acitivity are arranged in the repository cabinet；
Pass-through box is offered on the repository cabinet, the automatic transshipment acitivity can receive to be come from outside by pass-through box transmission
The transfer tank for having cryopreservation tube；And the automatic transshipment acitivity cryopreservation tube in the transfer tank received can be transferred to it is described
In profound hypothermia holding vessel.
2. biological sample repository according to claim 1, which is characterized in that
It is provided with the hand basket of moveable position in the profound hypothermia holding vessel, is provided with multiple tray supports in the hand basket,
Bracketing has cryopreservation tube pallet in the tray support；The profound hypothermia holding vessel is provided with storage door, each pallet branch
Support member can be with the movement of hand basket, to be moved to position corresponding with the storage door；
The automatic transshipment acitivity includes shovel sending component, and the shovel sending component may pass through storage door for the cryopreservation tube pallet in hand basket
It scoops up reception or received cryopreservation tube pallet can be shoveled and sent back in hand basket.
3. biological sample repository according to claim 2, which is characterized in that the shovel sending component include shovel send box body,
Shovel send the first actuator, shovel that the second actuator, shovel is sent to send sliding bottom, slide-and-guide plate, shovel disk component and pallet；
The shovel, which is sent, to be offered shovel and send delivery port on box body, and the shovel send delivery port can be with the storage of the profound hypothermia holding vessel
Door is corresponding；
The pallet and the shovel send sliding bottom to be arranged at the shovel and send in box body, and the slide-and-guide plate is slidable
Setting is sent on sliding bottom in the shovel, and the shovel disk component is slidably disposed on the slide-and-guide plate；
The pallet can keep in transfer tank；
The shovel, which is sent, to be offered shovel and send logical nitrogen hole on box body, the shovel send logical nitrogen hole to be connected to nitrogen supply system；
The shovel send the first actuator to drive the sliding of the slide-and-guide plate, and the shovel send the second actuator to shovel disk
The sliding of component is driven.
4. biological sample repository according to claim 3, which is characterized in that it includes left sliding that the shovel, which send sliding bottom,
Seat, right sliding seat and bedframe, the left sliding seat and right sliding seat are separately positioned on the left and right sides of the bedframe；
The slide-and-guide plate includes left slide-and-guide track, right slide-and-guide track and sliding panel；The left slide-and-guide rail
Road and right slide-and-guide track are separately positioned on the left and right sides end of the sliding panel；
The shovel disk component includes shoveling sliding part of facing left, the right sliding part of shovel disk and shovel disk；It is described to shovel face left sliding part and the shovel disk right side
The left and right sides of the shovel disk is arranged in sliding part；
The outside of the left slide-and-guide track is slided on the left sliding seat, and the outside of the right slide-and-guide track is in institute
It states and is slided on right sliding seat, the sliding part of facing left that shovels slides on the inside of the left slide-and-guide track, and the shovel disk is right
Sliding part upper sliding on the inside of the right slide-and-guide track.
5. biological sample repository according to claim 4, which is characterized in that
The left sliding seat includes sliding support plate and multiple pulleys, and the multiple pulley is in a row to be arranged in the sliding support
On plate；The right sliding seat and the left sliding holder structure are identical and arranged symmetrically；
The left slide-and-guide track is at I-shaped, the right slide-and-guide track and the left slide-and-guide track structure phase
It is same and arranged symmetrically；
It includes that shovel send driving motor and lead screw that the shovel, which send the first actuator, is provided with lead screw spiral shell on the sliding panel lower end surface
Mother, the feed screw nut are connect with threads of lead screw, and the shovel send the motor shaft of driving motor to connect with the lead screw.
6. biological sample repository according to claim 1, which is characterized in that the automatic transshipment acitivity further includes barcode scanning
Component, the barcode scanning component can carry out barcode scanning to the cryopreservation tube that it is transported.
7. according to biological sample repository described in claim 3,4 or 5, which is characterized in that the automatic transshipment acitivity also wraps
It includes transhipment and receives cabinet and manipulator；
The transhipment receives cabinet and the shovel send box body to be arranged side by side, the manipulator setting the shovel sending component with it is described
Transhipment receives the top of cabinet；
The transhipment receives cabinet and receives the transfer tank come in from pass-through box conveying；The manipulator can carry out transfer tank
Crawl；
The shovel, which is sent, to be offered shovel and send receiving port on cassette upper end face, the manipulator can be moved into send receiving port corresponding with shovel
Position.
8. biological sample repository according to claim 7, which is characterized in that the automatic transshipment acitivity further includes transhipment
Shell；The manipulator include crawl driving assembly, Z axis set, Z axis bar, Y-axis guide rail, the first X-axis guide rail, the second X-axis guide rail,
Zhua Guan mechanism and take pipe fitting；
First X-axis guide rail and the distribution of the second X-axis guide rail are fixed on the two side ends of the transhipment shell, the Y-axis guided way
Two side ends be slidably arranged in first X-axis guide rail and the second X-axis guide rail；The Z axis set is vertically arranged, and Z axis set
Middle part be slidably disposed on the Y-axis guided way；
The Z axis bar is slidably disposed in the Z axis set, and the lower end of the Z axis bar is provided with Z axis supporting, described to grab
Tank mechanism and pipe fitting is taken to be separately positioned on institute's Z axis supporting；
The Zhua Guan mechanism can grab transfer tank, described to take pipe fitting that carry out taking pipe to cryopreservation tube；
The crawl driving assembly respectively drives the movement of the Z axis set, Y-axis guide rail and Z axis bar.
9. according to claim 1, biological sample repository described in 2,3,4,5,6 or 8, which is characterized in that the profound hypothermia storage
It deposits and is provided with swivel mount, rotary drive assembly and lifting member in tank；
The lifting member is nested in the swivel mount, and the tank on the top of the lifting member and the profound hypothermia holding vessel
Body is mutually fixed；The rotary drive assembly drives swivel mount, may make swivel mount in profound hypothermia holding vessel around institute
Lifting member is stated to be rotated；
The swivel mount is divided into multiple storage areas by multiple reticles, offers promotion operating walk way on the storage area,
Each promotion operating walk way connects with the lifting member；
Hand basket is provided in the storage area；The hand basket periphery, which extends outwardly, is arranged a promotion reception block, and the promotion connects
It receives block and extends to position corresponding with the lifting member across the promotion operating walk way；
The lifting member can be by rising the effect driving hand basket for pushing reception block along operating walk way is promoted in storage area
Drop movement.
10. biological sample repository according to claim 9, which is characterized in that
The swivel mount upper center is recessed with downwards transition axial groove, and the periphery of the transition axial groove, which extends outwardly, to be provided with
Multiple demarcation plates, and multiple promotion operating walk ways are offered on the circumferential cell wall of the transition axial groove；The promotion operation is logical
The storage area that road and demarcation plate are divided into corresponds；
The lifting member include promoted sleeve, promoted driving motor, promoted driving wheel, promoted driven wheel, promoted transition wheel and
Lifter board；
The promotion sleeve is arranged in the transition axial groove, and can be with swivel mount relative rotation；It is described to promote the upper of sleeve
Portion's circumference is outward extended with promotion transition plates, and the promotion driving motor is arranged outside the profound hypothermia holding vessel, and described
The motor shaft for promoting driving motor, which extends downwardly the cover for sequentially passing through profound hypothermia holding vessel and promotes transition plates, is provided with promotion
Driving wheel；
The promotion sleeve periphery upper end is provided with promotion transition wheel and engages connection, the promotion sleeve with the promotion driving wheel
Peripheral lower end is provided with promotion driven wheel, is provided with promotion conveyer belt between the promotion driven wheel and the promotion transition wheel；
Lifter board is provided on the promotion transmission belt, the lifter board is corresponding with promotion reception block.
11. biological sample repository according to claim 10, which is characterized in that be provided with storage on the demarcation plate and lead
To dragging track, the hand basket periphery, which is also equipped with, is oriented to hand basket draw runner of the dragging track to cooperation with the storage, described to mention
Basket draw runner slides on storage guiding dragging track.
12. biological sample repository according to claim 10, which is characterized in that
It is described to promote the pipe sleeve that sleeve is inner hollow；
Turning effort axis, turning effort axis upper end are equipped with to promotion sleeve internal stretch at the slot bottom center of the transition axial groove
It is provided with " ten " word receiving slit；
The rotary drive assembly includes rotary drive motor and rotation drive shaft, and the rotary drive motor is fixed on the depth
Outside cryogenic storage tank, and the motor shaft of the rotary drive motor and the rotation drive shaft are coaxially connected, and the rotation is driven
Moving axis lower end is provided with " ten " word shackle member, and the shackle member for rotating drive shaft is fastened in receiving slit.
13. according to claim 1, biological sample repository described in 2,3,4,5,6,8,10,11 or 12, which is characterized in that institute
Hand basket is stated into sector, and the two sides in the hand basket are provided with multiple support bars, every two support bars in same level
Form the tray support.
14. biological sample repository according to claim 13, which is characterized in that the cryopreservation tube pallet be and hand basket phase
The sector agreed with, and be provided on the cryopreservation tube pallet it is multiple deposit tube seat, the slot bottom for depositing tube seat is exploitation formula slot bottom.
15. according to claim 1, biological sample repository described in 2,3,4,5,6,8,10,11,12 or 14, which is characterized in that
It further include maintenance door, nitrogen supply system and dehumidification system, the maintenance door is arranged on the repository cabinet, can pass through inspection
An opening repository cabinet is repaired, its inside is overhauled；
The nitrogen supply system can to being carried out in repository cabinet for nitrogen, can also in automatic transshipment acitivity for nitrogen；
The dehumidification system can dehumidify to repository cabinet.
16. a kind of storage method of biological sample repository, which is characterized in that
The pass-through box that the transfer tank for having cryopreservation tube directly passes through repository cabinet is put into repository by R step；
S step, it is low that the cryopreservation tube in the transfer tank received is transferred to the depth by automatic transshipment acitivity in repository automatically
In warm holding vessel.
17. the storage method of biological sample repository according to claim 16, which is characterized in that
The manipulator of S1 step, automatic transshipment acitivity grabs repository received transfer tank on the pallet of setting；
S2 step, while carrying out S1 step or after above-mentioned R step, automatic transshipment acitivity can be moved along given line
To at corresponding profound hypothermia holding vessel；
The storage door of S3 step, profound hypothermia holding vessel is opened, and the shovel sending component of automatic transshipment acitivity is in profound hypothermia holding vessel
Cryopreservation tube pallet shovels on the corresponding platform of automatic transshipment acitivity；
S4 step, the cryopreservation tube that manipulator grabs on pallet in transfer tank are placed on cryopreservation tube pallet；
Cryopreservation tube tray is transmitted back in profound hypothermia holding vessel by S5 step, shovel sending component again.
18. the storage method of biological sample repository according to claim 17, which is characterized in that
When the S4 step carries out, manipulator first grabs cryopreservation tube to barcode scanning component top on cryopreservation tube from pallet
Two dimensional code carry out barcode scanning, the control system of setting handled and stored to barcode scanning result, later again by cryopreservation tube grab to
On cryopreservation tube pallet.
19. the storage method of biological sample repository described in 7 or 18 according to claim 1, which is characterized in that
T step, before S3 step progress, the corresponding hand basket in profound hypothermia holding vessel is moved to opposite with storage door
The position answered；
The T step is divided into:
T1 step, the rotary drive assembly driving swivel mount rotation of profound hypothermia holding vessel, so that the hand basket on swivel mount be driven to exist
It moves upward in week at the corresponding position of storage door of profound hypothermia holding vessel；
T2 step, lifting member promote hand basket, and cryopreservation tube tray lift to the shovel sending component on its hand basket is made to may pass through storage
Door shovels the position sent to it.
20. the storage method of biological sample repository described in 6,17 or 18 according to claim 1, which is characterized in that further include G
Step: repository cabinet is directly directly transported by being equipped with the transport vehicle of container.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811330237.0A CN109368107A (en) | 2018-11-09 | 2018-11-09 | A kind of biological sample repository and its storage method |
| PCT/CN2019/070359 WO2020093585A1 (en) | 2018-11-09 | 2019-01-04 | Biological sample repository and storage method therefor |
| JP2021600063U JP3235995U (en) | 2018-11-09 | 2019-01-04 | Biological sample storage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811330237.0A CN109368107A (en) | 2018-11-09 | 2018-11-09 | A kind of biological sample repository and its storage method |
Publications (1)
| Publication Number | Publication Date |
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| CN109368107A true CN109368107A (en) | 2019-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811330237.0A Pending CN109368107A (en) | 2018-11-09 | 2018-11-09 | A kind of biological sample repository and its storage method |
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| Country | Link |
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| JP (1) | JP3235995U (en) |
| CN (1) | CN109368107A (en) |
| WO (1) | WO2020093585A1 (en) |
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| WO2020093585A1 (en) | 2020-05-14 |
| JP3235995U (en) | 2022-01-21 |
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