CN116555030B - Storage equipment based on umbilical cord mesenchymal stem cell culture - Google Patents
Storage equipment based on umbilical cord mesenchymal stem cell culture Download PDFInfo
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- CN116555030B CN116555030B CN202310686737.2A CN202310686737A CN116555030B CN 116555030 B CN116555030 B CN 116555030B CN 202310686737 A CN202310686737 A CN 202310686737A CN 116555030 B CN116555030 B CN 116555030B
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 53
- 238000004113 cell culture Methods 0.000 title claims abstract description 26
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 125
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 230000000712 assembly Effects 0.000 claims abstract description 12
- 238000000429 assembly Methods 0.000 claims abstract description 12
- 238000009434 installation Methods 0.000 claims abstract description 6
- 238000010257 thawing Methods 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 18
- 238000007664 blowing Methods 0.000 claims description 5
- 230000000903 blocking effect Effects 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 239000013078 crystal Substances 0.000 abstract description 12
- 230000004888 barrier function Effects 0.000 abstract description 6
- 238000009833 condensation Methods 0.000 abstract description 6
- 230000005494 condensation Effects 0.000 abstract description 6
- 239000007789 gas Substances 0.000 description 49
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 238000010586 diagram Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 238000010276 construction Methods 0.000 description 5
- 239000004744 fabric Substances 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 101150114468 TUB1 gene Proteins 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/91—Cell lines ; Processes using cell lines
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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Abstract
The invention relates to the field of cell culture, in particular to storage equipment based on umbilical cord mesenchymal stem cell culture. Technical problems: when the ice crystal formed on the outer surface of the reagent tube is wiped, it is necessary to repeatedly wipe a portion, and it is difficult for a person to identify whether the reagent tube is broken or not by wiping. The technical scheme is as follows: a storage device based on umbilical cord mesenchymal stem cell culture comprises a storage barrel, an installation disc, a lifting assembly and the like; the storage barrel is connected with a mounting disc in a sliding manner; the storage barrel is connected with lifting assemblies matched with the number of the through holes, and the lifting assemblies are connected with the mounting disc. Moisture in the gas conveyed by the external first external pump machine is removed through the conveying pipe, low-temperature gas is formed, a barrier is formed on the outer surface of the reagent pipe, indoor air is isolated, the phenomenon that water vapor condensation occurs on the outer surface of the reagent pipe after the indoor air contacts the reagent pipe is prevented, and then ice crystals are formed.
Description
Technical Field
The invention relates to the field of cell culture, in particular to storage equipment based on umbilical cord mesenchymal stem cell culture.
Background
The existing umbilical cord mesenchymal stem cells need to be filled into a reagent tube and then placed in a low-temperature environment for storage, in the transportation process of the mesenchymal stem cells, the reagent tube needs to be placed in a transportation box for storing liquid nitrogen for transportation, the mesenchymal stem cells are stored through the low temperature emitted by the liquid nitrogen, and when the mesenchymal stem cells need to be cultured, the reagent tube needs to be placed in water for thawing the mesenchymal stem cells.
Before thawing, the tube wall of the reagent tube is relatively thin and is made of glass, in the transportation process, the reagent tube is easy to damage due to factors such as shaking or collision, so that whether the reagent tube is damaged or not needs to be checked, the problem that after the thawing of the mesenchymal stem cells is finished, the mesenchymal stem cells are contacted with the outside air to cause pollution and can not be continuously cultured is avoided, however, the reagent tube is stored in an ultralow temperature environment, when taken out, the whole reagent tube is in an ultralow temperature state and is lower than the indoor air, when the reagent tube is contacted with the indoor air, the surface of the reagent tube is subjected to a water vapor condensation phenomenon, then ice crystals are formed under the influence of the ultralow temperature of the reagent tube, whether the reagent tube is damaged or not can not be identified is avoided, and the ice crystals on the surface of the reagent tube need to be wiped by using a wiping cloth manually, however, the wiped ice crystal can be reformed under the influence of ultralow temperature, so that whether the reagent tube is damaged or not needs to be identified manually and quickly, if not, wiping is needed to be repeated on one part, so that whether the reagent tube is damaged or not is difficult to identify manually in a wiping mode, in order to ensure the activity of the mesenchymal stem cells, thawing operation is needed within forty seconds to one minute after the reagent tube is taken out, the identification difficulty is greatly increased, the surface of the reagent tube is stuck with a label for marking the information of the mesenchymal stem cells, when the label is contacted with indoor air, the surface of the label is also subjected to a water vapor condensation phenomenon, ice crystals are formed under the influence of the ultralow temperature of the reagent tube and permeate into the label, so that the label is particularly moist, and when the label is wiped, the wiping cloth rubs with the label, the font on the label is easy to be blurred, and the label is easy to damage;
after the reagent tube is determined to be unbroken, the whole reagent tube is soaked in water, so that the whole label is in a soaked state in a thawing mode, the adhesive force of the adhesive on the label is easily lost, the edge tilting phenomenon occurs, the subsequent inconvenient transportation is caused, in the process of culturing the mesenchymal stem cells, the hydrolytic operation is required to be repeatedly carried out on the mesenchymal stem cells, and the adhesive on the label is easy to fall in the repeated cold-hot alternation process.
Disclosure of Invention
In order to overcome the defect that when ice crystals formed on the outer surface of a reagent tube are wiped, one part needs to be wiped repeatedly, and therefore whether the reagent tube is damaged or not is difficult to identify manually in a wiping mode, the invention provides a storage device based on umbilical mesenchymal stem cell culture.
The technical proposal is as follows: a storage device based on umbilical cord mesenchymal stem cell culture comprises a storage barrel, an installation disc, a plugging block and a lifting assembly; the storage barrel is provided with a first cavity; the storage barrel is provided with a second cavity; the second cavity is provided with a charging valve; the storage barrel is connected with a mounting disc in a sliding manner; the mounting disc is provided with a plurality of through holes; the mounting disc is rotationally connected with plugging blocks with the number matched with that of the through holes; a torsion spring is arranged between the plugging block and the mounting disc; the storage barrel is connected with lifting assemblies which are matched with the through holes in number, and the lifting assemblies are connected with the mounting disc and the plugging blocks; the device also comprises a conveying pipe, a collecting pipe, a first gas pipe and a wind guide pipe; the mounting disc is fixedly connected with a conveying pipe for cooling and dehumidifying the gas, an air inlet of the conveying pipe penetrates out of the mounting disc, and the air inlet of the conveying pipe is contacted with the inner wall of the storage barrel and is blocked by the inner wall of the storage barrel; the conveying pipe is communicated with a collecting pipe which is fixedly connected with the mounting disc; the mounting disc is fixedly connected with air guide pipes, the number of which is matched with that of the through holes, and the air guide pipes are positioned right above the corresponding through holes; the collecting pipe is connected with first air delivery pipes which are matched with the air guide pipes in number, and the first air delivery pipes are communicated with the corresponding air guide pipes.
Optionally, the duct is of a helical configuration for increasing the time for the gas to pass through the duct.
Optionally, the lifting assembly comprises a moving block, a first rope, a supporting plate, a first elastic piece, a clamping plate, a second elastic piece, a first pulley and a spring telescopic rod; the storage barrel is connected with a moving block in a sliding way; the storage barrel is fixedly connected with a second clamping block; the storage barrel is connected with a supporting plate in a sliding way; the supporting plate is provided with an arc concave part for supporting the reagent tube; the movable block is fixedly connected with a first rope, and the other end of the first rope sequentially penetrates through the second clamping block and the storage barrel and is fixedly connected with the supporting plate; the supporting plate is fixedly connected with at least one first elastic piece, and the first elastic piece is fixedly connected with the storage barrel; the storage barrel is connected with a clamping plate in a sliding way; the clamping plate is provided with a clamping pipe hole for fixing the middle part of the reagent pipe; the clamping plate is fixedly connected with at least one second elastic piece, and the second elastic piece is fixedly connected with the storage barrel; the storage barrel is provided with a first pulley; the installation disc is fixedly connected with a spring telescopic rod.
Optionally, the lifting assembly further comprises a pulling unit; the pulling unit comprises a second pulley, a second rope, a third rope, a first clamping block and a second clamping block; the telescopic part of the spring telescopic rod is fixedly connected with a second pulley; the supporting plate is fixedly connected with a second rope, and the other end of the second rope bypasses the first pulley and is fixedly connected with the second pulley; the mounting disc is fixedly connected with a first clamping block; the second pulley is fixedly connected with a third rope, and the other end of the third rope penetrates through the first clamping block and is fixedly connected with the plugging block.
Optionally, the device also comprises a push rod, a barrel cover and a clamping assembly; the storage barrel is fixedly connected with at least one push rod; the telescopic part of the push rod is fixedly connected with a barrel cover; the barrel cover is connected with clamping assemblies which are matched with the air guide pipes in number; the clamping assembly comprises a cylindrical connecting pipe, a turbine ventilator, a magnetic arc plate and an air outlet pipe; the barrel cover is fixedly connected with a cylindrical connecting pipe; the cylindrical connecting pipe is rotationally connected with a turbine ventilator; the turbine ventilator is connected with two magnetic arc plates for clamping the reagent tube in an adsorption mode, and the two magnetic arc plates are mutually adsorbed; the cylindrical connecting pipe is fixedly connected with an air outlet pipe, and the air outlet pipe is contacted with the turbine ventilator.
Optionally, the barrel cover is provided with a plurality of air collecting hoods, and each air collecting hood is located right below the corresponding turbine ventilator and used for collecting upwards the air blown out of the air guide pipe.
Optionally, the device also comprises a defrosting assembly; the defrosting assembly comprises a first telescopic pipe and a second air conveying pipe; the barrel cover is fixedly connected with a first telescopic pipe, and the other end of the first telescopic pipe penetrates out of the barrel cover; the first telescopic pipe is communicated with a plurality of second air delivery pipes, and the other ends of the second air delivery pipes penetrate through corresponding cylindrical connecting pipes to be communicated with the air outlet pipes.
Optionally, the thawing assembly further comprises a guide block; each air outlet pipe is provided with a guide block, and each guide block is provided with a guide part for guiding the air blown out by the second air pipe.
Optionally, a moisture removal assembly is also included; the barrel cover is connected with a plurality of moisture removing components, and the moisture removing components are connected with the corresponding cylindrical connecting pipes and the magnetic arc plates; the water removal assembly comprises an arc frame, a second telescopic pipe, an arc baffle and an air delivery pipe; a cavity is arranged in each magnetic arc plate; each magnetic arc plate is provided with a plurality of air outlet holes for removing moisture from the groove parts of the tube caps of the reagent tubes, and the air outlet holes are communicated with the cavities arranged in the corresponding cylindrical connecting tubes; an arc frame is fixedly connected between the barrel cover and the cylindrical connecting pipe; the arc-shaped frame is rotationally connected with an arc-shaped baffle; the arc-shaped frame and the arc-shaped baffle are mutually matched to form an air conveying channel; the second air pipe is communicated with an air conveying pipe, and the other end of the air conveying pipe passes through the arc-shaped frame and is communicated with the air conveying channel; the arc-shaped baffle plate is fixedly connected with two second telescopic pipes, the other end of each second telescopic pipe penetrates through the corresponding magnetic arc-shaped plate to be communicated with a cavity arranged in the cylindrical connecting pipe, and the second telescopic pipe is communicated with the air conveying channel.
Optionally, the air outlet is inclined upwards for blowing up moisture existing in the recess of the upper part of the reagent tube.
The invention has the following advantages: the method comprises the steps that moisture in gas conveyed by an external first external pump is removed through a conveying pipe, the temperature of the gas can be changed into low-temperature gas under the influence of the temperature of the conveying pipe, moisture does not exist in the gas blown out of an air guide pipe, the gas is low-temperature gas, a barrier is formed on the outer surface of a reagent pipe in the process that the gas is blown upwards around the outer surface of the reagent pipe under the influence of the air guide pipe, indoor air is isolated, after the indoor air is prevented from being contacted with the reagent pipe, the whole reagent pipe is in an ultralow temperature state and is lower than the indoor air, the phenomenon of water vapor condensation can occur on the outer surface of the reagent pipe, ice crystals are formed, and the ice crystals on the surface of the reagent pipe are required to be wiped by using wiping cloth manually, so that whether the reagent pipe is damaged or not can be identified;
when the gas passes through the turbine ventilator, the gas can push the turbine ventilator to rotate, and the turbine ventilator can drive the corresponding magnetic arc-shaped plate and the reagent tube to rotate, so that whether all parts on the outer surface of the reagent tube are damaged or not can be conveniently observed manually, the reagent tube does not need to be rotated manually, and the gas which can form a barrier on the outer surface of the reagent tube is utilized to drive the turbine ventilator to rotate, so that the use of electric energy can be reduced;
when the gas blown out from the gas outlet of the second gas pipe passes through the turbine ventilator, the turbine ventilator is pushed to rotate, so that the mesenchymal stem cells rotate in the reagent pipe, and the thawing time of the mesenchymal stem cells is shortened in a rotating mode.
Drawings
FIG. 1 is a schematic diagram of a first construction of a storage device based on umbilical cord mesenchymal stem cell culture according to the present invention;
FIG. 2 is a schematic diagram of a second construction of the storage device for umbilical cord mesenchymal stem cell culture according to the present invention;
FIG. 3 is a schematic diagram of a third embodiment of the umbilical cord mesenchymal stem cell culture-based storage device of the present invention;
FIG. 4 is a schematic view of a first construction of a moisture removal assembly and a closure block disclosed in the umbilical cord mesenchymal stem cell culture-based storage device of the present invention;
FIG. 5 is a schematic view of a second construction of the moisture removal assembly and the plugging block disclosed in the umbilical cord mesenchymal stem cell culture-based storage device of the present invention;
FIG. 6 is a schematic diagram of the structure of the delivery tube, the header, the first gas tube and the air guide tube disclosed by the storage device based on umbilical cord mesenchymal stem cell culture of the present invention;
FIG. 7 is a schematic view showing a first construction of a clamping assembly, a thawing assembly and a moisture removal assembly disclosed in the umbilical cord mesenchymal stem cell culture-based storage device of the present invention;
FIG. 8 is a schematic diagram of a second configuration of the clamping assembly, thawing assembly and moisture removal assembly disclosed in the umbilical cord mesenchymal stem cell culture-based storage device of the present invention;
FIG. 9 is a schematic diagram of a third configuration of the clamping assembly, thawing assembly and moisture removal assembly disclosed in the umbilical cord mesenchymal stem cell culture-based storage device of the present invention;
FIG. 10 is a schematic diagram showing the structure of a reagent vessel according to the present invention.
The marks of the components in the drawings are as follows: 1-storage barrel, 2-mounting disc, 3-plugging block, 4-push rod, 5-barrel cover, 6-conveying pipe, 7-collecting pipe, 8-first conveying pipe, 9-guide pipe, 101-moving block, 102-first rope, 103-supporting plate, 104-first elastic piece, 105-clamping plate, 106-second elastic piece, 107-first pulley, 108-spring telescopic rod, 109-second pulley, 1010-second rope, 1011-third rope, 1012-first clamping block, 1013-second clamping block, 201-cylindrical connecting pipe, 202-turbo ventilator, 203-magnetic arc plate, 204-air outlet pipe, 301-first telescopic pipe, 302-second conveying pipe, 303-guide block, 401-arc frame, 402-second telescopic pipe, 403-arc baffle, 404-air conveying pipe, 1 a-first cavity, 1 b-second cavity, 2 a-through hole, 105 a-clamping pipe, 103 a-arc concave, 203 a-air outlet hole, 303 a-guide part, 403 a-air conveying channel.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The storage equipment based on umbilical cord mesenchymal stem cell culture comprises a storage barrel 1, an installation disc 2, a plugging block 3 and a lifting assembly, wherein the storage barrel is shown in figures 1-6; the storage barrel 1 is provided with a first cavity 1a for storing liquid nitrogen; the storage barrel 1 is provided with a second cavity 1b for storing a reagent tube; the second cavity 1b is provided with a charging valve; the storage barrel 1 is connected with a mounting disc 2 in a sliding way; the mounting disc 2 is provided with eight through holes 2a; eight plugging blocks 3 for plugging the through holes 2a are rotatably connected with the mounting disc 2; a torsion spring is arranged between the plugging block 3 and the mounting disc 2; the storage barrel 1 is connected with eight lifting assemblies, and the lifting assemblies are connected with the mounting disc 2 and the plugging block 3;
the device also comprises a conveying pipe 6, a collecting pipe 7, a first air conveying pipe 8 and an air guide pipe 9; the mounting disc 2 is connected with a conveying pipe 6 for cooling and dehumidifying the gas through bolts, an air inlet of the conveying pipe 6 penetrates out of the mounting disc 2, and the air inlet of the conveying pipe 6 is contacted with the inner wall of the storage barrel 1 and is blocked by the inner wall of the storage barrel 1; the conveying pipe 6 is communicated with a collecting pipe 7, and the collecting pipe 7 is fixedly connected with the mounting disc 2; eight air guide pipes 9 are fixedly connected to the mounting disc 2, the air guide pipes 9 are positioned right above the corresponding through holes 2a, and the hollow areas of the air guide pipes 9 are communicated with the through holes 2a; the collecting pipe 7 is connected with eight first air delivery pipes 8, and the first air delivery pipes 8 are communicated with corresponding air guide pipes 9.
The conveyer pipe 6 is the helicitic texture for increase the time of gas through conveyer pipe 6, when the gas that then external first external pump machine carried was through conveyer pipe 6, can have longer condensation time, then can be better condense the gas that carries, and through the mode that increases the elapsed time, can let the better formation low temperature gas of gas that carries.
The lifting assembly comprises a moving block 101, a first rope 102, a supporting plate 103, a first elastic piece 104, a clamping plate 105, a second elastic piece 106, a first pulley 107 and a spring telescopic rod 108; the storage barrel 1 is connected with a moving block 101 in a sliding way; the storage barrel 1 is fixedly connected with a second clamping block 1013; the storage barrel 1 is connected with a supporting plate 103 in a sliding way; the support plate 103 is provided with an arc recess 103a for holding the reagent tube; the moving block 101 is fixedly connected with a first rope 102, and the other end of the first rope 102 sequentially passes through the second clamping block 1013 and the storage barrel 1 and is fixedly connected with the supporting plate 103; the supporting plate 103 is fixedly connected with two first elastic pieces 104, and the first elastic pieces 104 are fixedly connected with the storage barrel 1; the storage barrel 1 is connected with a clamping plate 105 in a sliding manner, and the clamping plate 105 is positioned right above the supporting plate 103; the clamping plate 105 is provided with a clamping tube hole 105a for fixing the middle part of the reagent tube; the clamping plate 105 is fixedly connected with two second elastic pieces 106, and the second elastic pieces 106 are fixedly connected with the storage barrel 1; the storage tub 1 is provided with a first pulley 107; the mounting disc 2 is fixedly connected with a spring telescopic rod 108.
The lifting assembly further comprises a pulling unit; the pulling unit comprises a second pulley 109, a second rope 1010, a third rope 1011, a first clamping block 1012 and a second clamping block 1013; a second pulley 109 is fixedly connected to the telescopic part of the spring telescopic rod 108; the supporting plate 103 is fixedly connected with a second rope 1010, and the other end of the second rope 1010 bypasses the first pulley 107 and is fixedly connected with the second pulley 109; the mounting disc 2 is fixedly connected with a first clamping block 1012; the second pulley 109 is fixedly connected with a third rope 1011, and the other end of the third rope 1011 passes through the first clamping block 1012 and is fixedly connected with the plugging block 3.
The specific operation of the above example 1 is as follows: before a reagent tube filled with mesenchymal stem cells is placed in the second cavity 1b, firstly, a charging valve arranged on the first cavity 1a is opened to convey liquid nitrogen into the first cavity 1a, and at the moment, the low temperature emitted by the liquid nitrogen in the first cavity 1a is transferred into the second cavity 1b, so that the second cavity 1b is in a low-temperature environment, and thus, the preparation work is prepared;
when the reagent tube containing the mesenchymal stem cells is placed in the second cavity 1b, as shown in fig. 5, a lifting assembly is described below, firstly, the moving block 101 is driven to move downwards by electric power, when moving, the moving block 101 drives the supporting plate 103 to move upwards by the first rope 102, in the process, when the supporting plate 103 contacts with the clamping plate 105, the clamping plate 105 is pushed to move upwards by the supporting plate 103, when the clamping plate 105 contacts with the mounting disc 2, the clamping plate 105 pushes the mounting disc 2 and the connected parts thereof to move upwards, then the moving block 101 stops moving, at this time, the first elastic member 104 is in a stretching state, the second elastic member 106 is in a compression state, and note that, in the process of moving the supporting plate 103 upwards, the second pulley 109 is driven to move downwards by the second rope 1010, and the second pulley 109 pulls the sealing block 3 to rotate by the third rope 1011, the blocking of the through hole 2a is removed, the telescopic part of the spring telescopic rod 108 is in an extending state, then a reagent tube is manually put in from the hollow area of the air guide pipe 9, the reagent tube sequentially passes through the through hole 2a and the clamping pipe hole 105a, then the bottom of the reagent tube contacts with the circular arc concave part 103a, then the reagent tube slowly moves upwards again through the electric control moving block 101, in the process, the supporting plate 103 loses the pulling force of the first rope 102, under the driving of the first elastic piece 104 and the second elastic piece 106, the disc 2, the supporting plate 103, the clamping plate 105 and the reagent tube are firstly installed to move downwards, in the moving process, the disc 2, the clamping plate 105 and the supporting plate 103 are sequentially restored to the original positions, and at the moment, the bottom of the reagent tube is supported by the supporting plate 103, the middle part of the reagent tube is limited by the clamping plate 105 and is stably positioned in the second cavity 1b;
it should be noted that, in the process of moving the supporting plate 103 downward, the second rope 1010 is loosened, the telescopic part of the spring telescopic rod 108 is slowly retracted, so as to drive the second pulley 109 to move upward, at this time, the third rope 1011 is in a relaxed state, the blocking block 3 is slowly restored to the original position under the driving of the torsion spring, and in this process, since the blocking block 3 is slowly restored to the original position, the reagent tube is not broken due to strong collision when contacting with the reagent tube, so as to complete the operation of placing the reagent tube into the second cavity 1b, and the rest of the lifting components place the reagent tube into the second cavity 1b according to the same manner as above, after the placement is completed, the telescopic part of the push rod 4 is retracted, so as to drive the barrel cover 5 and the clamping component to move downward, so that the barrel cover 5 contacts with the storage barrel 1;
it should be noted that before the culture of the mesenchymal stem cells in the reagent tube is required, as shown in fig. 3, the lifting assembly is controlled to drive the corresponding reagent tube to perform the same operation as when the reagent tube containing the mesenchymal stem cells is placed in the second cavity 1b, the moving block 101 drives the supporting plate 103 to move upwards, so that the reagent tube sequentially passes through the through hole 2a and the hollow area of the air guide tube 9, the upper part of the reagent tube is not located in the second cavity 1b, and in the moving process, the supporting plate 103 also sequentially pushes the clamping plate 105 and the mounting disc 2 to move upwards, so as to complete the removal of the reagent tube from the second cavity 1b, at this moment, the lower part of the mounting disc 2 is located at the upper edge of the supporting plate 1, the air inlet of the conveying tube 6 is located above the storage barrel 1, the air inlet of the conveying tube 6 is not contacted with the inner wall of the storage barrel 1, at this moment, the inner wall of the storage barrel 1 is not blocked, the clamping plate 105 is attached to the mounting disc 2, and the clamping plate 103 is attached to the clamping plate 105;
meanwhile, in the process that the supporting plate 103 drives the reagent tube to move upwards, the air is conveyed into the collecting pipe 7 from the air inlet of the conveying tube 6 through the external first external pump, then is split into all the first air conveying tubes 8, and then is blown out from the corresponding air guide tubes 9, the air blown out from the air guide tubes 9 can be blown upwards around the outer surfaces of the reagent tube under the influence of the air guide tubes 9, at the moment, because the conveying tube 6 is positioned in the mounting disc 2, the bottom of the mounting disc 2 is positioned on the upper surface of the second cavity 1b, the conveying tube 6 is in a low-temperature state, when the air passes through the conveying tube 6, the moisture in the air can be condensed by the conveying tube 6 to realize the removal of the moisture in the air, the temperature of the air can be changed into low-temperature air under the influence of the temperature of the conveying tube 6, and the air blown out from the air guide tubes 9 cannot exist, and is low temperature gas, then in the process of blowing up the gas from the outer surface of the air guide pipe 9 around the reagent pipe, a barrier is formed on the outer surface of the reagent pipe, the indoor air is isolated, after the indoor air is prevented from contacting the reagent pipe, because the whole reagent pipe is in an ultralow temperature state and lower than the indoor air, the moisture condensation phenomenon can occur on the outer surface of the reagent pipe, then ice crystals are formed, the ice crystals on the surface of the reagent pipe are required to be wiped manually by using a wiping cloth, whether the reagent pipe is damaged or not can be identified, and the secondary formation of the ice crystals can be prevented by the way that the cold gas blown out from the air guide pipe 9 forms the barrier on the outer surface of the reagent pipe, and note that dust can not occur in the channel of the conveying pipe 6 because the initial state of the air inlet of the conveying pipe 6 is blocked by the inner wall of the storage barrel 1, when dust appears in the channel of the conveying pipe 6, the air blown out by the air guide pipe 9 is attached to the outer surface of the reagent pipe, so that the outer surface of the reagent pipe is blurred, and whether the reagent pipe is damaged or not is influenced by manual identification;
it should be noted that, because each lifting component can independently drive the corresponding reagent tube to be taken out from the second cavity 1b, thawing operation can be independently carried out on the mesenchymal stem cells of one reagent tube, the thawing operation is required within forty seconds to one minute after the reagent tube is taken out is avoided, the time is too short, and other mesenchymal stem cells are not thawed timely, so that the effect of the mesenchymal stem cells is caused, and the disc 2 is not required to be opened, the phenomenon that the low-temperature gas in the second cavity 1b overflows when the reagent tube is taken out, so that excessive volatilization of liquid nitrogen in the first cavity 1a is caused, and the inner diameter of the reagent tube through hole 2a is equal to the outer diameter of the reagent tube, so that the second cavity 1b is still in a relatively sealed state, the phenomenon that low-temperature cold air in the second cavity 1b overflows from the through hole 2a large amount, so that the temperature of the second cavity 1b is increased, so that the effect of the mesenchymal stem cells in other reagent tubes is influenced, and the lifting component is in the second cavity 1b is not driven, so that the second cavity 1b cannot be started up due to the fact that the low-temperature component 1b is not driven in the second cavity is not used;
it should be noted that, because the conveying pipe 6 is of a spiral structure, the time that the conveying pipe 6 passes through is increased for the gas conveyed by the first external pump, when the gas conveyed by the external first external pump passes through the conveying pipe 6, the longer condensing time can be provided, the moisture in the conveyed gas can be better condensed, and the conveyed gas can be better formed into low-temperature gas by increasing the passing time.
Example 2
On the basis of the embodiment 1, as shown in fig. 1-3 and 7-9, the device also comprises a push rod 4, a barrel cover 5 and a clamping assembly; the storage barrel 1 is connected with two push rods 4 through bolts; the telescopic parts of all the push rods 4 are fixedly connected with a barrel cover 5; eight clamping assemblies for clamping the reagent tubes are connected to the barrel cover 5, and the clamping assemblies are located right above the corresponding air guide pipes 9; the clamping assembly comprises a cylindrical connecting pipe 201, a turbine ventilator 202, a magnetic arc-shaped plate 203 and an air outlet pipe 204; the barrel cover 5 is fixedly connected with a cylindrical connecting pipe 201; the cylindrical connecting pipe 201 is rotatably connected with a turbine ventilator 202; the turbine ventilator 202 is connected with two magnetic arc plates 203 for clamping the reagent tube in an adsorption manner, and the two magnetic arc plates 203 are mutually adsorbed; the cylindrical connecting pipe 201 is fixedly connected with an air outlet pipe 204, and the air outlet pipe 204 is contacted with the turbine ventilator 202.
The barrel cover 5 is provided with a plurality of air collecting hoods, each air collecting hood is located under a corresponding turbine ventilator 202 and used for collecting the air blown out of the air guide pipe 9 upwards, and the air blown out of the air guide pipe 9 is collected through the air collecting hoods, so that the air passing through the turbine ventilator 202 can be increased, and the turbine ventilator 202 can rotate stably.
The specific operation of the above example 2 is as follows: it should be noted that, after the reagent tube containing the mesenchymal stem cells is placed in the second cavity 1b, the telescopic part of the push rod 4 is retracted to drive the barrel cover 5, the clamping assembly and the parts connected with the barrel cover 5 to move downwards, so that the barrel cover 5 is attached to the storage barrel 1, and before the reagent tube is taken out from the second cavity 1b, the telescopic part of the push rod 4 is required to extend to push the barrel cover 5 and the clamping assembly to move upwards, so that the barrel cover 5 is far away from the storage barrel 1;
it should be noted that after the supporting plate 103 pushes the reagent tube to move upwards and sequentially pass through the through hole 2a and the hollow area of the air guide pipe 9, the upper part of the reagent tube contacts with the two corresponding magnetic arc plates 203, then the reagent tube pushes the two corresponding magnetic arc plates 203 to move away from each other, and then the two magnetic arc plates 203 approach each other under the influence of magnetic force to clamp the upper part of the reagent tube;
it should be noted that, after the air blown from the air guide pipe 9 is blown upwards, the air passes through the corresponding turbine ventilator 202 and then is blown out from the air outlet pipe 204, and when the air passes through the turbine ventilator 202, the air pushes the turbine ventilator 202 to rotate, and the turbine ventilator 202 drives the corresponding magnetic arc plate 203 and the reagent pipe to rotate, so that it is convenient to observe manually whether all parts on the outer surface of the reagent pipe are damaged, the reagent pipe does not need to be rotated manually, and the air blown from the air guide pipe 9 is used for driving the turbine ventilator 202 to rotate in a mode that the barrier is formed on the outer surface of the reagent pipe, so that the use of electric energy can be reduced, and as shown in fig. 9, when the air blown from the air guide pipe 9 is blown upwards, the air is converged by the air converging cover when passing through the turbine ventilator 202, and then the air blown from the air guide pipe 9 is converged by the air converging cover, so that the air passing through the turbine ventilator 202 can be increased, and the air passing through the turbine ventilator 202 can be stably rotated.
Example 3
On the basis of the embodiment 2, as shown in fig. 1-3 and 7-9, the thawing device is further included; the defrosting assembly comprises a first telescopic pipe 301 and a second air pipe 302; the barrel cover 5 is fixedly connected with a first telescopic pipe 301, and the other end of the first telescopic pipe 301 penetrates out of the barrel cover 5; the first telescopic pipe 301 is communicated with eight second air delivery pipes 302, and the other ends of the second air delivery pipes 302 penetrate through corresponding cylindrical connecting pipes 201 to be communicated with the air outlet pipes 204.
The defrosting assembly also comprises a guide block 303; each of the air outlet pipes 204 is provided with a guide block 303, and each guide block 303 is provided with a guide portion 303a for guiding the air blown out from the second air delivery pipe 302.
The specific operation of the above example 3 is as follows: after the reagent tube is determined to be unbroken, firstly, gas between thirty-five degrees and thirty-seven degrees is shunted into all second gas delivery tubes 302 through the first telescopic tube 301 by the second external pump, and finally the gas is blown out from the corresponding second gas delivery tubes 302;
it should be noted that, the gas blown out from the second gas pipe 302 blows downwards around the outer surface of the reagent pipe under the influence of the corresponding turbine ventilator 202, and when the gas passes through the reagent pipe, the gas defreezes the mesenchymal stem cells in the reagent pipe, so that the heated gas is utilized to defreeze the mesenchymal stem cells, the whole reagent pipe is not required to be soaked in water for defreezing, and the glue on the label is prevented from losing adhesion due to the way of thawing by soaking the whole reagent pipe in water, thereby affecting the subsequent transportation;
it should be noted that, when the gas blown out from the second gas pipe 302 passes through the turbo ventilator 202, the turbo ventilator 202 is pushed to rotate, so that the mesenchymal stem cells rotate in the reagent pipe, the thawing time of the mesenchymal stem cells is shortened in a rotating manner, and the gas blown out from the gas outlet of the second gas pipe 302 is blown down intensively under the influence of the guiding portion 303a, so that the rotation speed of the turbo ventilator 202 is increased, and the thawing time of the mesenchymal stem cells is better shortened.
Example 4
Based on the embodiment 3, as shown in fig. 7-10, a moisture removal assembly is also included; eight moisture removing components are connected with the barrel cover 5 and are connected with the corresponding cylindrical connecting pipe 201 and the magnetic arc plate 203; the moisture removing assembly comprises an arc-shaped frame 401, a second telescopic pipe 402, an arc-shaped baffle 403 and an air conveying pipe 404; a cavity is arranged in each magnetic arc plate 203; each magnetic arc plate 203 is provided with a plurality of air outlet holes 203a for removing moisture from the groove parts of the tube caps of the reagent tubes, and the air outlet holes 203a are communicated with the cavities arranged in the corresponding cylindrical connecting tubes 201; an arc-shaped frame 401 is fixedly connected between the barrel cover 5 and the cylindrical connecting pipe 201; the arc frame 401 is rotatably connected with an arc baffle 403; the arc-shaped frame 401 and the arc-shaped baffle 403 are mutually matched to form an air conveying channel 403a; the second air delivery pipe 302 is communicated with an air delivery pipe 404, and the other end of the air delivery pipe 404 passes through the arc-shaped frame 401 and is communicated with an air delivery channel 403a; the arc baffle 403 is fixedly connected with two second telescopic pipes 402, and the other end of each second telescopic pipe 402 passes through the corresponding magnetic arc plate 203 to be communicated with a cavity arranged in the cylindrical connecting pipe 201, and the second telescopic pipe 402 is communicated with the air conveying channel 403 a.
The air outlet 203a is inclined upwards and is used for blowing the moisture existing in the concave part of the upper part of the reagent tube upwards, so that the air blown out from the air outlet 203a can push the moisture existing in the concave part of the upper part of the reagent tube to move upwards and rapidly away from the concave part of the upper part of the reagent tube.
The specific operation of the above example 4 is as follows: it should be noted that when the reagent tube containing the mesenchymal stem cells is placed in the second cavity 1b, the telescopic part of the push rod 4 can be controlled to extend to push the barrel cover 5 and all the clamping components, the thawing component and the moisture removing component to move upwards, then the reagent tube is placed in the corresponding two magnetic arc plates 203, the reagent tube is clamped by the mutual matching of the two magnetic arc plates 203, at this time, each air outlet 203a is opposite to the recess of the upper part of the reagent tube corresponding to the air outlet, and in the process of blowing out the gas from the gas outlet of the second gas pipe 302 by the second external pump through the first telescopic tube 301, part of the air is transferred into the air transfer channel 403a through the air transfer tube 404 and then transferred into the cavity arranged in the two cylindrical connecting tubes 201 through the second telescopic tube 402, finally, the air blown out from the air outlet 203a will remove the moisture in the recess at the upper part of the reagent tube as shown in fig. 10, so as to avoid that when the moisture exists in the recess at the upper part of the reagent tube in the process of the mesenchymal stem cells, the moisture existing in the recess at the upper part of the reagent tube will freeze into ice cubes after being directly put into the second cavity 1b, the ice cubes are continuously in low temperature, the moisture in the second cavity 1b will be absorbed continuously, the ice cubes formed will become bigger and bigger than the through hole 2a, so that the reagent tube cannot be taken out, and it should be noted that, because the air outlet 203a is inclined upwards, the air blown out from the air outlet 203a can push the moisture existing in the recess at the upper part of the reagent tube to move upwards, quickly away from the recess at the upper part of the reagent tube.
The foregoing is merely exemplary of the present invention and is not intended to limit the present invention. All equivalents and alternatives falling within the spirit of the invention are intended to be included within the scope of the invention. What is not elaborated on the invention belongs to the prior art which is known to the person skilled in the art.
Claims (7)
1. The storage equipment based on umbilical cord mesenchymal stem cell culture comprises a storage barrel (1), an installation disc (2), a plugging block (3) and a lifting assembly; the storage barrel (1) is provided with a first cavity (1 a); the storage barrel (1) is provided with a second cavity (1 b); the second cavity (1 b) is provided with a charging valve; the storage barrel (1) is connected with a mounting disc (2) in a sliding way; the mounting disc (2) is provided with a plurality of through holes (2 a); the mounting disc (2) is rotationally connected with plugging blocks (3) which are matched with the through holes (2 a) in number; a torsion spring is arranged between the blocking block (3) and the mounting disc (2); the storage barrel (1) is connected with lifting assemblies which are matched with the through holes (2 a) in number, and the lifting assemblies are connected with the mounting disc (2) and the plugging blocks (3); the method is characterized in that: the device also comprises a conveying pipe (6), a collecting pipe (7), a first air conveying pipe (8) and an air guide pipe (9); the mounting disc (2) is fixedly connected with a conveying pipe (6) for cooling and dehumidifying the gas, an air inlet of the conveying pipe (6) penetrates out of the mounting disc (2), and the air inlet of the conveying pipe (6) is contacted with the inner wall of the storage barrel (1) and is blocked by the inner wall of the storage barrel (1); the conveying pipe (6) is communicated with a collecting pipe (7), and the collecting pipe (7) is fixedly connected with the mounting disc (2); the mounting disc (2) is fixedly connected with air guide pipes (9) which are matched with the through holes (2 a) in number, and the air guide pipes (9) are positioned right above the corresponding through holes (2 a); the collecting pipe (7) is connected with first air delivery pipes (8) which are matched with the air guide pipes (9) in number, and the first air delivery pipes (8) are communicated with the corresponding air guide pipes (9);
the device also comprises a push rod (4), a barrel cover (5) and a clamping assembly; at least one push rod (4) is fixedly connected with the storage barrel (1); a barrel cover (5) is fixedly connected with the telescopic part of the push rod (4); the barrel cover (5) is connected with clamping assemblies which are matched with the air guide pipes (9) in number; the clamping assembly comprises a cylindrical connecting pipe (201), a turbine ventilator (202), a magnetic arc plate (203) and an air outlet pipe (204); the barrel cover (5) is fixedly connected with a cylindrical connecting pipe (201); the cylindrical connecting pipe (201) is rotationally connected with a turbine ventilator (202); the turbine ventilator (202) is connected with two magnetic arc plates (203) for clamping the reagent tube in an adsorption mode, and the two magnetic arc plates (203) are mutually adsorbed; the cylindrical connecting pipe (201) is fixedly connected with an air outlet pipe (204), and the air outlet pipe (204) is contacted with the turbine ventilator (202);
the thawing device also comprises a thawing assembly; the defrosting assembly comprises a first telescopic pipe (301) and a second air conveying pipe (302); the barrel cover (5) is fixedly connected with a first telescopic pipe (301), and the other end of the first telescopic pipe (301) penetrates out of the barrel cover (5); the first telescopic pipe (301) is communicated with a plurality of second air delivery pipes (302), and the other ends of the second air delivery pipes (302) penetrate through corresponding cylindrical connecting pipes (201) to be communicated with the air outlet pipes (204);
the thawing assembly also comprises a guide block (303); each air outlet pipe (204) is provided with a guide block (303), and each guide block (303) is provided with a guide part (303 a) for guiding air blown out of the second air conveying pipe (302).
2. The umbilical cord mesenchymal stem cell culture-based storage device of claim 1, wherein: the conveying pipe (6) is of a spiral structure and is used for increasing the time for the gas to pass through the conveying pipe (6).
3. The umbilical cord mesenchymal stem cell culture-based storage device of claim 1, wherein: the lifting assembly comprises a moving block (101), a first rope (102), a supporting plate (103), a first elastic piece (104), a clamping plate (105), a second elastic piece (106), a first pulley (107) and a spring telescopic rod (108); the storage barrel (1) is connected with a moving block (101) in a sliding way; the storage barrel (1) is fixedly connected with a second clamping block (1013); the storage barrel (1) is connected with a supporting plate (103) in a sliding way; the supporting plate (103) is provided with an arc concave part (103 a) for supporting the reagent tube; the moving block (101) is fixedly connected with a first rope (102), and the other end of the first rope (102) sequentially passes through the second clamping block (1013) and the storage barrel (1) and is fixedly connected with the supporting plate (103); the supporting plate (103) is fixedly connected with at least one first elastic piece (104), and the first elastic piece (104) is fixedly connected with the storage barrel (1); the storage barrel (1) is connected with a clamping plate (105) in a sliding way; the clamping plate (105) is provided with a clamping pipe hole (105 a) for fixing the middle part of the reagent pipe; the clamping plate (105) is fixedly connected with at least one second elastic piece (106), and the second elastic piece (106) is fixedly connected with the storage barrel (1); the storage barrel (1) is provided with a first pulley (107); the installation disc (2) is fixedly connected with a spring telescopic rod (108).
4. A storage device based on umbilical cord mesenchymal stem cell culture as claimed in claim 3, wherein: the lifting assembly further comprises a pulling unit; the pulling unit comprises a second pulley (109), a second rope (1010), a third rope (1011), a first clamping block (1012) and a second clamping block (1013); the telescopic part of the spring telescopic rod (108) is fixedly connected with a second pulley (109); the supporting plate (103) is fixedly connected with a second rope (1010), and the other end of the second rope (1010) bypasses the first pulley (107) and is fixedly connected with the second pulley (109); the mounting disc (2) is fixedly connected with a first clamping block (1012); the second pulley (109) is fixedly connected with a third rope (1011), and the other end of the third rope (1011) passes through the first clamping block (1012) to be fixedly connected with the plugging block (3).
5. The umbilical cord mesenchymal stem cell culture-based storage device of claim 1, wherein: the barrel cover (5) is provided with a plurality of air collecting hoods, each air collecting hood is located under the corresponding turbine ventilator (202) and used for collecting air blown out of the air guide pipe (9) upwards.
6. The umbilical cord mesenchymal stem cell culture-based storage device of claim 1, wherein: the water removing component is also included; the barrel cover (5) is connected with a plurality of moisture removing components, and the moisture removing components are connected with the corresponding cylindrical connecting pipes (201) and the magnetic arc plates (203); the water removal assembly comprises an arc-shaped frame (401), a second telescopic pipe (402), an arc-shaped baffle (403) and an air delivery pipe (404); a cavity is arranged in each magnetic arc plate (203); each magnetic arc plate (203) is provided with a plurality of air outlet holes (203 a) for removing moisture from the groove parts of the tube caps of the reagent tubes, and the air outlet holes (203 a) are communicated with the cavities arranged in the corresponding cylindrical connecting tubes (201); an arc-shaped frame (401) is fixedly connected between the barrel cover (5) and the cylindrical connecting pipe (201); the arc-shaped frame (401) is rotatably connected with an arc-shaped baffle (403); the arc-shaped frame (401) and the arc-shaped baffle (403) are mutually matched to form an air conveying channel (403 a); the second air delivery pipe (302) is communicated with an air delivery pipe (404), and the other end of the air delivery pipe (404) passes through the arc-shaped frame (401) to be communicated with an air delivery channel (403 a); the arc-shaped baffle (403) is fixedly connected with two second telescopic pipes (402), the other end of each second telescopic pipe (402) passes through the corresponding magnetic arc-shaped plate (203) to be communicated with a cavity arranged in the cylindrical connecting pipe (201), and the second telescopic pipe (402) is communicated with the air conveying channel (403 a).
7. The umbilical cord mesenchymal stem cell culture-based storage device of claim 6, wherein: the air outlet hole (203 a) is inclined upwards and is used for blowing the moisture existing in the concave part at the upper part of the reagent tube upwards.
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