CN109362473A - The implantation methods of vinifera - Google Patents
The implantation methods of vinifera Download PDFInfo
- Publication number
- CN109362473A CN109362473A CN201811580724.2A CN201811580724A CN109362473A CN 109362473 A CN109362473 A CN 109362473A CN 201811580724 A CN201811580724 A CN 201811580724A CN 109362473 A CN109362473 A CN 109362473A
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- China
- Prior art keywords
- nutrient solution
- vinifera
- implantation methods
- value
- grape
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Cultivation Of Plants (AREA)
- Hydroponics (AREA)
Abstract
The present invention relates to a kind of implantation methods of vinifera, including step (1): the grape Rooted Cuttings of health are rinsed and are disinfected, it is placed into the hydroponic box containing nutrient solution A and is cultivated, the temperature that the process culture controls nutrient solution is 15-18 DEG C, oxygen content is 4mg/L-6mg/L, EC value is 0.6ms/cm-1.1ms/cm, and replacement in nutrient solution A every five days is primary；Step (2): culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and it is persistently sprayed to the fruit very large phase daily, this process control solution temperature is 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/cm-1.1ms/cm.The implantation methods of vinifera provided by the invention have the characteristics that method is simple and significant effect, and it can effectively adjust the anthocyanin accumulation of grapevine seedling, to improve the content of anthocyanidin, the vinifera produced has the characteristics that growth cycle is short fastly, anthocyanidin content is high.
Description
Technical field
The invention belongs to water culture technical fields, and in particular to a kind of implantation methods of vinifera.
Background technique
Contain a large amount of anthocyanidin in grape, and anthocyanidin belongs to bioflavonoids, and flavonoid substance is most important
Physiological active functions are free radical scavenging ability and oxidation resistance, and anthocyanidin not only makes plant that multicoloured color be presented,
Also there is the activity for reducing enzyme, the different bioactive molecule for waiting healthcare functions of resistance, in addition, with the development of science and technology, people are to food
The safety of additive is increasingly paid attention to, and the development and utilization of natural additive have become the general trend that Development of Additives uses.
Vinifera, which refers to, mainly produces the grape variety of purpose to make grape wine.Be broadly divided into red cultivar,
Candida species etc..North China, Bay areas, Xinjiang, Gansu, Ningxia etc. are distributed in the main vinifera producing region in China
Ground.And the direction that the anthocyanidin in vinifera then develops at present vinifera is improved, and the life of existing vinifera
There is not the more preferable method for improving anthocyanidin in vinifera in production.
Summary of the invention
To solve problems of the prior art, the present invention provides a kind of implantation methods of vinifera, according to
Sequencing the following steps are included:
Step (1): the grape Rooted Cuttings of health are rinsed and are disinfected, the water planting containing nutrient solution A is placed into
It is cultivated in case, the temperature which controls nutrient solution is 15-18 DEG C, and oxygen content 4mg/L-6mg/L, EC value is
Replacement in 0.6ms/cm-1.1ms/cm, nutrient solution A every five days is primary；
Step (2): culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and persistently sprays daily
To the fruit very large phase, this process control solution temperature is 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/
cm-1.1ms/cm；
The nutrient solution A includes calcium nitrate, potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate, copper sulphate, sodium molybdate, chelated iron；
The nutrient solution B includes chitin, compound sodium nitrophenolate, uniconazole P；Wherein
100~200mg/L of calcium nitrate, 350~450mg/L of potassium nitrate, 200~300mg/L of potassium dihydrogen phosphate, magnesium sulfate 50
~80mg/L, 0.3~0.7mg/L of copper sulphate, 50~80mg/L of pumpkin powder, 0.01~0.03mg/L of sodium molybdate, chelated iron 20~
25mg/L, 0.8~1.5mg/L of chitin, 4~6mg/L of compound sodium nitrophenolate, 1~3mg/L of uniconazole P.
Preferably, each component including following content: calcium nitrate 200mg/L, potassium nitrate 450mg/L, potassium dihydrogen phosphate
300mg/L, magnesium sulfate 80mg/L, copper sulphate 0.7mg/L, pumpkin powder 80mg/L, sodium molybdate 0.03mg/L, chelated iron 25mg/L,
Chitin 1.5mg/L, compound sodium nitrophenolate 6mg/L, uniconazole P 3mg/L.
It preferably, further include pumpkin powder in the nutrient solution A and the nutrient solution B.
In any of the above-described scheme preferably, the pH value of the nutrient solution A is 5.4-6.4.
In any of the above-described scheme preferably, in the step (1), the thimerosal for disinfecting selection is mass fraction
For 0.3% potassium permanganate solution.
In any of the above-described scheme preferably, the copper sulphate is cupric sulfate pentahydrate.
In any of the above-described scheme preferably, the sodium molybdate is Sodium Molybdate Dihydrate.
In any of the above-described scheme preferably, the 1/2-2/3 of the grape Rooted Cuttings root system is dipped in the battalion when culture
In nutrient solution A.
In any of the above-described scheme preferably, the hydroponic box setting ventilation mouth.
In any of the above-described scheme preferably, whose training case is made of transparent glass.
The implantation methods of vinifera provided by the invention have the characteristics that method is simple and significant effect, and can have
Effect adjusts the anthocyanin accumulation of grapevine seedling, to improve the content of anthocyanidin, the vinifera produced has growth cycle
Short feature fast, anthocyanidin content is high.
Specific embodiment
In order to be further understood that summary of the invention of the invention, the present invention is elaborated below in conjunction with specific embodiment.
Embodiment one
1000mlA liquid includes each component of following content: calcium nitrate 125mg/L, potassium nitrate 375mg/L, biphosphate
Potassium 225mg/L, magnesium sulfate 55mg/L, cupric sulfate pentahydrate 0.4mg/L, pumpkin powder 55mg/L, Sodium Molybdate Dihydrate 0.01mg/L, chelating
Iron 22.5mg/L；
1000ml B liquid includes each component of following content: pumpkin powder 55mg/L, chitin 1mg/L, compound sodium nitrophenolate
4.5mg/L, uniconazole P 1.5mg/L.
By health grape Rooted Cuttings be rinsed and using 0.3% potassium permanganate solution impregnate root 5min into
Row disinfection removes potassium permanganate residual with sterile distilled water rinse after disinfection, the grape Rooted Cuttings of sanitized is placed into and are contained
It is cultivated in the hydroponic box of nutritious liquid A, the 1/2-2/3 of root system is dipped in nutrient solution A, which controls nutrient solution
Temperature is 15-18 DEG C, and oxygen content 4mg/L-6mg/L, EC value is 0.6ms/cm-1.1ms/cm, replacement one in nutrient solution A every five days
It is secondary；Culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and is persistently sprayed to the fruit very large phase daily,
This process control solution temperature is 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/cm-1.1ms/cm.
Embodiment two
1000ml A liquid includes each component of following content: calcium nitrate 100mg/L, potassium nitrate 35mg/L, biphosphate
Potassium 200mg/L, magnesium sulfate 50mg/L, cupric sulfate pentahydrate 0.3mg/L, pumpkin powder 50mg/L, Sodium Molybdate Dihydrate 0.01mg/L, chelating
Iron 20mg/L；
1000ml B liquid includes each component of following content: pumpkin powder 5mg/L, chitin 0.8mg/L, compound sodium nitrophenolate 4mg/
L, uniconazole P 1mg/L.
By health grape Rooted Cuttings be rinsed and using 0.3% potassium permanganate solution impregnate root 5min into
Row disinfection removes potassium permanganate residual with sterile distilled water rinse after disinfection, the grape Rooted Cuttings of sanitized is placed into and are contained
It is cultivated in the hydroponic box of nutritious liquid A, the 1/2-2/3 of root system is dipped in nutrient solution A, which controls nutrient solution
Temperature is 15-18 DEG C, and oxygen content 4mg/L-6mg/L, EC value is 0.6ms/cm-1.1ms/cm, replacement one in nutrient solution A every five days
It is secondary；Culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and is persistently sprayed to the fruit very large phase daily,
This process control solution temperature is 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/cm-1.1ms/cm.
Embodiment three
1000ml A liquid includes each component of following content: calcium nitrate 150mg/L, potassium nitrate 400mg/L, biphosphate
Potassium 250mg/L, magnesium sulfate 65mg/L, cupric sulfate pentahydrate 0.55mg/L, pumpkin powder 65mg/L, Sodium Molybdate Dihydrate 0.02mg/L, chela
Close iron 22.5mg/L；
1000ml B liquid includes each component of following content: pumpkin powder 65mg/L, chitin 1.2mg/L, compound sodium nitrophenolate
5mg/L, uniconazole P 2mg/L.
By health grape Rooted Cuttings be rinsed and using 0.3% potassium permanganate solution impregnate root 5min into
Row disinfection removes potassium permanganate residual with sterile distilled water rinse after disinfection, the grape Rooted Cuttings of sanitized is placed into and are contained
It is cultivated in the hydroponic box of nutritious liquid A, the 1/2-2/3 of root system is dipped in nutrient solution A, which controls nutrient solution
Temperature is 15-18 DEG C, and oxygen content 4mg/L-6mg/L, EC value is 0.6ms/cm-1.1ms/cm, replacement one in nutrient solution A every five days
It is secondary；Culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and is persistently sprayed to the fruit very large phase daily,
This process control solution temperature is 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/cm-1.1ms/cm.
Example IV
1000mlA liquid includes each component of following content: calcium nitrate 175mg/L, potassium nitrate 375mg/L, biphosphate
Potassium 275mg/L, magnesium sulfate 75mg/L, cupric sulfate pentahydrate 0.6mg/L, pumpkin powder 75mg/L, Sodium Molybdate Dihydrate 3mg/L, chelated iron
24.5mg/L；
1000ml B liquid includes each component of following content: pumpkin powder 75mg/L, chitin 1.25mg/L, compound sodium nitrophenolate
5.5mg/L, uniconazole P 2.5mg/L.
By health grape Rooted Cuttings be rinsed and using 0.3% potassium permanganate solution impregnate root 5min into
Row disinfection removes potassium permanganate residual with sterile distilled water rinse after disinfection, the grape Rooted Cuttings of sanitized is placed into and are contained
It is cultivated in the hydroponic box of nutritious liquid A, the 1/2-2/3 of root system is dipped in nutrient solution A, which controls nutrient solution
Temperature is 15-18 DEG C, and oxygen content 4mg/L-6mg/L, EC value is 0.6ms/cm-1.1ms/cm, replacement one in nutrient solution A every five days
It is secondary；Culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and is persistently sprayed to the fruit very large phase daily,
This process control solution temperature is 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/cm-1.1ms/cm.
Embodiment five
1000mlA liquid includes each component of following content: calcium nitrate 200mg/L, potassium nitrate 450mg/L, biphosphate
Potassium 300mg/L, magnesium sulfate 80mg/L, cupric sulfate pentahydrate 0.7mg/L, pumpkin powder 80mg/L, Sodium Molybdate Dihydrate 0.03mg/L, chelating
Iron 25mg/L；
1000ml B liquid includes each component of following content: pumpkin powder 80mg/L, chitin 1.5mg/L, compound sodium nitrophenolate
6mg/L, uniconazole P 3mg/L.
By health grape Rooted Cuttings be rinsed and using 0.3% potassium permanganate solution impregnate root 5min into
Row disinfection removes potassium permanganate residual with sterile distilled water rinse after disinfection, the grape Rooted Cuttings of sanitized is placed into and are contained
It is cultivated in the hydroponic box of nutritious liquid A, the 1/2-2/3 of root system is dipped in nutrient solution A, which controls nutrient solution
Temperature is 15-18 DEG C, and oxygen content 4mg/L-6mg/L, EC value is 0.6ms/cm-1.1ms/cm, replacement one in nutrient solution A every five days
It is secondary；Culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and is persistently sprayed to the fruit very large phase daily,
This process control solution temperature is 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/cm-1.1ms/cm.
Experimental result
Multiple grape Rooted Cuttings are divided into two groups, one group prepares the progress of grape Rooted Cuttings special culture solution using embodiment five
Culture (uses above-mentioned cultural method), is cultivated for another group using existing water planting liquid on the market and (uses common water planting
Liquid uses plant growth regulator as B liquid and uses cultural method same as five groups of embodiment as above-mentioned A liquid), it is right
It is respectively 35 DEG C by solvent, Extracting temperature of 70% ethyl alcohol that culture, which obtains grape, solid-to-liquid ratio 1:5, extraction time 30min,
Anthocyanidin in grape pip is extracted, then is purified by column chromatography, using the yield of anthocyanidin and purity as weighing apparatus
Figureofmerit, concrete outcome are as shown in the table:
The content of anthocyanidin in the grape that the different water planting liquid of table 1 are turned out
| Group | Anthocyanidin yield | Purity |
| Embodiment five | 2.1% | 96.2% |
| Common water planting liquid | 1.6% | 90.7% |
It will be apparent to those skilled in the art that the implantation methods of vinifera of the invention include aforementioned present invention specification
Summary of the invention and specific embodiment part, it is as space is limited and each without constituting these combinations to keep specification concise
Scheme describes one by one.All within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should all wrap
Containing within protection scope of the present invention.
Claims (9)
1. a kind of implantation methods of vinifera, according to sequencing the following steps are included:
Step (1): the grape Rooted Cuttings of health are rinsed and are disinfected, are placed into the hydroponic box containing nutrient solution A
It is cultivated, the temperature which controls nutrient solution is 15-18 DEG C, and oxygen content 4mg/L-6mg/L, EC value is 0.6ms/
Replacement in cm-1.1ms/cm, nutrient solution A every five days is primary；
Step (2): culture continues to spray nutrient solution B comprehensively to the plant after growth after two weeks, and is persistently sprayed to fruit daily
Real very large phase, this process control solution temperature are 18-20 DEG C, and oxygen content 5mg/L-7mg/L, EC value is 0.6ms/cm-
1.1ms/cm；
The nutrient solution A includes calcium nitrate, potassium nitrate, potassium dihydrogen phosphate, magnesium sulfate, copper sulphate, sodium molybdate, chelated iron；
The nutrient solution B includes chitin, compound sodium nitrophenolate, uniconazole P.
2. the implantation methods of vinifera according to claim 1, which is characterized in that the nutrient solution A and the nutrition
It further include pumpkin powder in liquid B.
3. the implantation methods of vinifera according to claim 1, which is characterized in that the pH value of the nutrient solution A is
5.4-6.4。
4. the implantation methods of vinifera according to claim 1, which is characterized in that in the step (1), disinfection treatment
The thimerosal of selection is the potassium permanganate solution that mass fraction is 0.3%.
5. the implantation methods of vinifera according to claim 1, which is characterized in that the copper sulphate is five water sulfuric acid
Copper.
6. the implantation methods of vinifera according to claim 1, which is characterized in that the sodium molybdate is molybdate dihydrate acid
Sodium.
7. the implantation methods of vinifera according to claim 1, which is characterized in that grape Rooted Cuttings root when culture
The 1/2-2/3 of system is dipped in the nutrient solution A.
8. the implantation methods of vinifera according to claim 1, which is characterized in that the hydroponic box setting ventilation mouth.
9. the implantation methods of vinifera according to claim 1, which is characterized in that whose training case is transparent glass system
At.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811580724.2A CN109362473A (en) | 2018-12-24 | 2018-12-24 | The implantation methods of vinifera |
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| CN201811580724.2A CN109362473A (en) | 2018-12-24 | 2018-12-24 | The implantation methods of vinifera |
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| CN109362473A true CN109362473A (en) | 2019-02-22 |
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| CN201811580724.2A Pending CN109362473A (en) | 2018-12-24 | 2018-12-24 | The implantation methods of vinifera |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110291975A (en) * | 2019-06-28 | 2019-10-01 | 西北农林科技大学 | A kind of sand culture vinifera nutrient solution prescription, preparation method and inoculation method |
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Application publication date: 20190222 |