CN109287852A - A method of animal semen quality is improved using chestnut flower water extract - Google Patents
A method of animal semen quality is improved using chestnut flower water extract Download PDFInfo
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- CN109287852A CN109287852A CN201811085090.3A CN201811085090A CN109287852A CN 109287852 A CN109287852 A CN 109287852A CN 201811085090 A CN201811085090 A CN 201811085090A CN 109287852 A CN109287852 A CN 109287852A
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- A—HUMAN NECESSITIES
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- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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Abstract
The present invention relates to a kind of methods for being improved animal semen quality using chestnut flower water extract, chestnut flower water extract is supplemented by stomach-filling or the mode being added in feed daily after weaning to boar, small-sized boar intake dosage is 0.01-20mg/kg weight, medium-sized boar intake dosage is 0.1-20mg/kg feed, and the chestnut flower water extract is the powder for the freeze-drying that further hyperfiltration obtains after ultrasonic wave extraction.Present invention firstly provides utilization ultrasonic wave extraction chestnut flower water extracts after further hyperfiltration, the semen quality of animal can be improved significantly to boar supplementary feeding, supplementary feeding opportunity is i.e. beginning after boar wean, technical solution of the present invention enables boar preferably to establish reproductive system, the genetic potential for sufficiently excavating male animal, extends the utilization scope of chestnut flower water extract.
Description
Technical field
The present invention relates to animals' reproduction physiology and reproduction technique field, and in particular to a kind of to be mentioned using chestnut flower water extract
The method of high animal semen quality.
Background technique
In recent years, lead to male due to the excess of agriculture chemical or incorrect application, environmental pollution, climate change etc.
The decline of animal sperm vigor, sperm concentration reduce, rate of teratosperm increases, and significantly mention so as to cause the infertile rate of male
Height, this is a global problem, causes global extensive attention.However good method improves currently not yet
Semen quality is to infertile to treat male.Therefore, scientists all improve hero finding effective drug or method
The semen quality of property animal.
Chinese chestnut (Castanea mollissima BL.), also known as: chestnut, Chinese chestnut, stalwart chestnut, Mao Li, wind chestnut, be known as " dry fruit it
King ", " panax ginseng fruit " good reputation.Chestnut it is full of nutrition, be up to 70.1% containing sugar and starch in fruit, protein 7%.In addition,
Also fatty, calcium, phosphorus, iron, the content of a variety of vitamins and microelement, especially vitamin C, B1 and carrotene are more general
Dry fruit is all high.Chestnut is full of nutrition, in addition to rich in starch, contains monosaccharide and disaccharide, carotene, thiamine, riboflavin, Ni Ke
The nutriments such as acid, ascorbic acid, protein, fat, inorganic salts.Chinese medicine thinks that chestnut has kidney and spleen invigorating, keeps fit and healthy, benefit
Stomach calming the liver and other effects, referred to as " fruit of kidney ".Recent study discovery chestnut flower in have many effective components, have it is anti-oxidant,
Anti-aging, anti-inflammatory and other effects, however whether the effective component in chestnut flower also has effects that improvement semen quality is also unknown,
There is no any document to disclose about the method for improving semen quality using chestnut flower water extract.
Summary of the invention
To solve the above problems, the present invention proposes a kind of side for improving animal semen quality using chestnut flower water extract
Method safely and effectively improves animal semen quality, and it is an object of the present invention to provide a kind of have using the chestnut flower water extract of natural component
Method.
The method for improving animal semen quality using chestnut flower water extract of the invention, it is critical that male lactation is dynamic
Chestnut flower water extract is supplemented by stomach-filling or the mode being added in feed daily after object wean, small-sized boar is taken the photograph
Entering dosage is 0.01-20mg/kg weight, and medium-sized boar intake dosage is 0.1-20mg/kg feed.
Further, the preparation step of the chestnut flower water extract is as follows:
1) Chinese Chestnut for picking fresh full-bloom stage is placed into ultrapure water immediately, and solid-liquid ratio 35g/L is filled with refiner
Slurry chestnut flower is distributed equally, then the ultrasonic extraction 1h in 45 DEG C, the ultrasonic instrument of power 780W, subsequent 12000r/min centrifugation
50min collects water layer solution, and obtained water layer solution is freeze-dried, buff powder is obtained；
2) buff powder that step 1) obtains is dissolved in again in ultrapure water, the ultra micro mistake for being 0.25 μm using aperture
Filter membrane filters, and will filter obtained aqueous solution and be freeze-dried again and obtain the solid powder of chestnut flower water extract；
3) solid powder for the chestnut flower water extract that step 2) obtains 4 DEG C are placed in save backup.
Further, when the small-sized boar is mouse, chestnut flower water extract, institute are supplemented since 21 ages in days
Chestnut flower water extract is stated to be supplemented by stomach-filling mode.
Further, when the boar is pig, chestnut flower water extract, the Chinese chestnut are supplemented since 35 ages in days
Flower water extract is supplemented by the mode being added in feed daily.
Further, when the boar is sheep, chestnut flower water extract, the plate are supplemented since 65 ages in days
Chestnut flower water extract is supplemented by the mode being added in feed daily.
Preferably, when the small-sized boar is mouse, the chestnut flower water extract daily intake is
10mg/kg weight.
Preferably, when the boar is pig, the chestnut flower water extract daily intake is 10mg/kg feeding
Material.
Preferably, when the boar is sheep, the chestnut flower water extract daily intake is 10mg/kg
Feed.
Preferably, it is Qingdao Huang Island Chestnut Orchard that the chestnut flower, which derives from,.
Beneficial effects of the present invention are as follows:
1, it is put forward for the first time the essence that can improve animal significantly to boar supplementary feeding using chestnut flower water extract
Liquid quality, supplementary feeding opportunity are i.e. beginning after boar wean.The quality of sperm is not only related with the trophic level of male animal,
It is more related with the budding developmental state of male animal, through present inventor team for many years the study found that just from the male animal puberty
Start to carry out manual intervention, can preferably establish reproductive system, sufficiently excavates the genetic potential of male animal.Using technology of the invention
The chestnut flower water extract that scheme obtains is analyzed by using high performance liquid chromatography-mass spectrometry technology, and discovery main component is
Water-soluble biological bases, a small amount of protein, carbohydrate, microelement etc., and found after present inventor detects, from plate
The water-soluble biological bases chemical structure that Li Huazhong is extracted is similar to mammal estrogen chemical structure, can be with mammal
Intracorporal estrogen receptor combines, and estrogen receptor signal path is caused to generate variation, promotes reproduction of male animal system hair
It educates, promotes the generation of sperm.Therefore, it is the mammal supplementary feeding chestnut flower water extract after wean, obtains sexual maturation
The technical effect that semen quality significantly improves afterwards.
2, the utilization scope of chestnut flower water extract is extended.Prior art, chestnut flower water extract are basically used for resisting
Bacterium is antibacterial, also it has been reported that chestnut flower male inflorescence water extract has antitumous effect.And the application is found through experiments that
Chestnut flower water extract is supplemented since the puberty, animal sperm vigor and density can be improved, and do not change the abnormal of sperm
Form quotient or acrosomal integnity.
3, the complete method for extracting chestnut flower water extract and the method for different animals are given.The skill of the application
Art scheme proposes chestnut flower after ultrasonic wave extraction, just for being supplemented to animal after filtering using further ultrafiltration membrane.
Ultrafiltration technology eliminates more impurity, and these impurity are possible to have side effect to puberty male animal building reproductive system.For
Different animals, the application method and dosage of chestnut flower water extract are all different.The application passes through a large number of experiments, has finally determined
Application method and dosage on mouse, pig and sheep.
Detailed description of the invention
Fig. 1 is the sperm concentration column comparison diagram that mouse supplements different replenishers amount chestnut flower water extracts
Fig. 2 is the sperm motility column comparison diagram that mouse supplements different replenishers amount chestnut flower water extracts
Fig. 3 is the sperm concentration column comparison diagram that pig supplements different replenishers amount chestnut flower water extracts
Fig. 4 is the sperm motility column comparison diagram that pig supplements different replenishers amount chestnut flower water extracts
Fig. 5 is the sperm concentration column comparison diagram that sheep supplements different replenishers amount chestnut flower water extracts
Fig. 6 is the sperm motility column comparison diagram that sheep supplements different replenishers amount chestnut flower water extracts
Specific embodiment
Below by way of specific embodiment further illustrate technical solution of the present invention, but technical solution of the present invention not with
Embodiment is limited.
Embodiment 1: chestnut flower water extract is prepared
1) Chinese Chestnut for picking fresh full-bloom stage is placed into ultrapure water immediately, and solid-liquid ratio 35g/L is filled with refiner
Slurry chestnut flower is distributed equally, then the ultrasonic extraction 1h in 45 DEG C, the ultrasonic instrument of power 780W, subsequent 12000r/min centrifugation
50min collects water layer solution, and obtained water layer solution is freeze-dried, buff powder is obtained；
2) buff powder that step 1) obtains is dissolved in again in ultrapure water, the ultra micro mistake for being 0.25 μm using aperture
Filter membrane filters, and will filter obtained aqueous solution and be freeze-dried again and obtain the solid powder of chestnut flower water extract；
3) solid powder for the chestnut flower water extract that step 2) obtains 4 DEG C are placed in save backup.
Embodiment 2: mouse feeding experiment
The 3 week old mouse (weight uniformity) 240 of healthy ICR strain, stochastic averagina are assigned to 8 groups (30/group),
Mouse is all normal feed and drinking-water, and is raised in unified SPF grade mouse room.It is weighed daily by the design of table 1 certain
Chestnut flower water extract powder obtained by 1 step 3) of embodiment of amount, is dissolved in pure water, is configured to required chestnut flower water and extracts
Object solution.Daily on time by way of stomach-filling, the chestnut flower water extract solution that matches to every mouse gavaging and weight.
Every intragastric administration on mice 0.1mL chestnut flower water extract solution.After stomach-filling 5 weeks, the sperm of mouse is collected, is analyzed with semen quality
Instrument analyzes sperm motility and density.Test result is as shown in table 1:
1 mouse of table supplements influence of the chestnut flower water extract to semen quality
| The dosage of daily iron supplement chestnut flower water extract | Sperm concentration (× 10^8) | Sperm motility (%) |
| Control group | 3.32 | 70.4 |
| Experimental group 1 (0.01mg/kg) | 3.45 | 72.4 |
| Experimental group 2 (0.1mg/kg) | 3.69 | 73.9 |
| Experimental group 3 (1mg/kg) | 3.76 | 76.5 |
| Experimental group 4 (5mg/kg) | 3.89 | 82.3 |
| Experimental group 5 (10mg/kg) | 4.12 | 85.4 |
| Experimental group 6 (15mg/kg) | 4.02 | 84.8 |
Research find within the scope of 0.01-10mg/kg have dose dependent (as concentration increases, effect is more obvious,
10mg/kg is best dosage), 15-20mg/kg and 10mg/kg effect do not have difference, as Fig. 1 mouse sperm density and Fig. 2 are small
Shown in mouse sperm motility.
Embodiment 3: swine rearing test
Cooperate Experimental Base in this school, chooses 70 health wean (35 age in days) boars, be randomly divided into 7 groups (10/group).
All pigs are all normal feed and drinking-water in one big feeding room, and are being managed collectively.It is weighed daily by the design of table 2
Chestnut flower water extract powder obtained by a certain amount of 1 step 3) of embodiment, weighed animal fine fodder and chestnut flower water extract
Powder is sufficiently mixed uniformly.The feed fine fodder prepared containing chestnut flower water extract feeds pig on time daily.It feeds to 6
A monthly age collects the sperm of pig, analyzes with semen quality analyzer sperm motility and density.
2 pig of table supplements influence of the chestnut flower water extract to semen quality
| The dosage of daily iron supplement chestnut flower water extract | Sperm concentration (× 10^9) | Sperm motility (%) |
| Control group | 2.05 | 68.9 |
| Experimental group 1 (0.1mg/kg feed) | 2.16 | 72.1 |
| Experimental group 2 (1mg/kg feed) | 2.29 | 74.5 |
| Experimental group 3 (5mg/kg feed) | 2.45 | 78.6 |
| Experimental group 4 (10mg/kg feed) | 2.56 | 85.7 |
| Experimental group 5 (15mg/kg feed) | 2.5 | 84.1 |
| Experimental group 6 (20mg/kg feed) | 2.47 | 83.8 |
Experiment discovery within the scope of 0.1-10mg/kg have dose dependent (as concentration increases, effect is more obvious,
10mg/kg is best dosage), 15-20mg/kg and 10mg/kg effect do not have difference, such as Fig. 3 Boar spermatozoa density and Fig. 4 pig essence
Shown in sub- vigor.
Embodiment 4: sheep feeding experiment
This school cooperate Experimental Base, choose 70 health wean (65 age in days) rams, be randomly divided into 7 groups (10/
Group).All sheep are all normal feed and drinking-water in one big feeding room, and are being managed collectively.It is set daily by table 3
Meter weighs chestnut flower water extract powder obtained by a certain amount of 1 step 3) of embodiment, weighed animal fine fodder and chestnut flower water
Extract powder is sufficiently mixed uniformly.The feed fine fodder prepared containing chestnut flower water extract feeds silk floss on time daily
Sheep guarantees that every animal has the chestnut flower water extract feed to match with feed intake.7.5-8 monthly age is fed, is collected
The sperm of sheep analyzes sperm motility and density with semen quality analyzer.
3 sheep of table supplements influence of the chestnut flower water extract to semen quality
Experiment discovery within the scope of 0.5-10mg/kg have dose dependent (as concentration increases, effect is more obvious,
10mg/kg is best dosage), 15-20mg/kg and 10mg/kg effect do not have difference, as Fig. 5 sheep sperm density and Fig. 6 are continuous
Shown in sheep sperm motility.
Above to a kind of method progress for improving animal semen quality using chestnut flower water extract provided by the present invention
It is discussed in detail.The principle of the present invention and embodiment are expounded herein by specific embodiment, described above
It is to be used to help understand method and its core concept of the invention.It should be pointed out that for those skilled in the art
For, it without departing from the principle of the present invention, can be with several improvements and modifications are made to the present invention, these improve and repair
Decorations are also fallen within the protection scope of the claims of the present invention.
Claims (9)
1. a kind of method for improving animal semen quality using chestnut flower water extract, which is characterized in that boar is disconnected
Chestnut flower water extract is supplemented by stomach-filling or the mode being added in feed daily after milk, small-sized boar takes in agent
Amount is 0.01-20mg/kg weight, and medium-sized boar intake dosage is 0.1-20mg/kg feed.
2. the method for improving animal semen quality using chestnut flower water extract according to claim 1, which is characterized in that institute
The preparation step for stating chestnut flower water extract is as follows:
1) Chinese Chestnut for picking fresh full-bloom stage is placed into ultrapure water immediately, solid-liquid ratio 35g/L, sufficiently even with refiner
Chestnut flower being starched, then the ultrasonic extraction 1h in 45 DEG C, the ultrasonic instrument of power 780W, subsequent 12000r/min is centrifuged 50min,
Water layer solution is collected, obtained water layer solution is freeze-dried, buff powder is obtained；
2) buff powder that step 1) obtains is dissolved in again in ultrapure water, the hyperfiltration film for being 0.25 μm using aperture
It filters, obtained aqueous solution will be filtered be freeze-dried again and obtain the solid powder of chestnut flower water extract；
3) solid powder for the chestnut flower water extract that step 2) obtains 4 DEG C are placed in save backup.
3. the method for improving animal semen quality using chestnut flower water extract according to claim 1, which is characterized in that institute
State small-sized boar be mouse when, since 21 ages in days supplement chestnut flower water extract, the chestnut flower water extract
It is supplemented by stomach-filling mode.
4. the method for improving animal semen quality using chestnut flower water extract according to claim 1, which is characterized in that institute
When to state boar be pig, chestnut flower water extract is supplemented since 35 ages in days, the chestnut flower water extract passes through every
It is added to the mode in feed and is supplemented.
5. the method for improving animal semen quality using chestnut flower water extract according to claim 1, which is characterized in that institute
When to state boar be sheep, chestnut flower water extract is supplemented since 65 ages in days, the chestnut flower water extract passes through
The mode being added in feed daily is supplemented.
6. according to claim 1 or 3 methods for improving animal semen quality using chestnut flower water extract, feature exist
In when the small-sized boar is mouse, the chestnut flower water extract daily intake is 10mg/kg weight.
7. according to claim 1 or 4 methods for improving animal semen quality using chestnut flower water extract, feature exist
In when the boar is pig, the chestnut flower water extract daily intake is 10mg/kg feed.
8. according to claim 1 or 5 methods for improving animal semen quality using chestnut flower water extract, feature exist
In when the boar is sheep, the chestnut flower water extract daily intake is 10mg/kg feed.
9. the method for improving animal semen quality using chestnut flower water extract according to claim 2, which is characterized in that institute
Chestnut flower is stated to derive from as Qingdao Huang Island Chestnut Orchard.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115606710A (en) * | 2022-09-08 | 2023-01-17 | 湖南文和友乔口小龙虾养殖有限公司 | Feed additive for crayfish and preparation method and application thereof |
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2018
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| US20140343134A1 (en) * | 2011-12-23 | 2014-11-20 | Horphag Reserach IP (QR) LTD | Ellagitannins Rich Extracts Composition in Sexual Wellness |
| CN102715352A (en) * | 2012-05-11 | 2012-10-10 | 北京农学院 | Application of chestnut involucre extract in improving health and growth performance of livestock and poultry |
| CN105998103A (en) * | 2016-07-19 | 2016-10-12 | 河北科技师范学院 | Chestnut flower activated extract and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115606710A (en) * | 2022-09-08 | 2023-01-17 | 湖南文和友乔口小龙虾养殖有限公司 | Feed additive for crayfish and preparation method and application thereof |
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