CN109280713A - 用于检测无细胞的病原体特异性核酸的方法和试剂盒 - Google Patents
用于检测无细胞的病原体特异性核酸的方法和试剂盒 Download PDFInfo
- Publication number
- CN109280713A CN109280713A CN201811203829.6A CN201811203829A CN109280713A CN 109280713 A CN109280713 A CN 109280713A CN 201811203829 A CN201811203829 A CN 201811203829A CN 109280713 A CN109280713 A CN 109280713A
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- Prior art keywords
- nucleic acid
- target nucleic
- dna
- double
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161470774P | 2011-04-01 | 2011-04-01 | |
| US61/470,774 | 2011-04-01 | ||
| CN201280026842.3A CN103814139B (zh) | 2011-04-01 | 2012-04-02 | 用于检测无细胞的病原体特异性核酸的方法和试剂盒 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280026842.3A Division CN103814139B (zh) | 2011-04-01 | 2012-04-02 | 用于检测无细胞的病原体特异性核酸的方法和试剂盒 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109280713A true CN109280713A (zh) | 2019-01-29 |
Family
ID=46932429
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811203829.6A Pending CN109280713A (zh) | 2011-04-01 | 2012-04-02 | 用于检测无细胞的病原体特异性核酸的方法和试剂盒 |
| CN201280026842.3A Expired - Fee Related CN103814139B (zh) | 2011-04-01 | 2012-04-02 | 用于检测无细胞的病原体特异性核酸的方法和试剂盒 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280026842.3A Expired - Fee Related CN103814139B (zh) | 2011-04-01 | 2012-04-02 | 用于检测无细胞的病原体特异性核酸的方法和试剂盒 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20140147851A1 (https=) |
| EP (1) | EP2694680A4 (https=) |
| JP (2) | JP6430826B2 (https=) |
| CN (2) | CN109280713A (https=) |
| AU (3) | AU2012236109A1 (https=) |
| RU (1) | RU2644672C2 (https=) |
| WO (1) | WO2012135815A2 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114480691A (zh) * | 2022-01-24 | 2022-05-13 | 广州迪澳基因科技有限公司 | 基于熔解曲线检测结核分枝杆菌复合菌群的方法及试剂盒 |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2694680A4 (en) * | 2011-04-01 | 2014-09-10 | Occam Biolabs Inc | METHOD AND KITS FOR DETECTION OF CELL-FREE NURVIVAL-SPECIFIC NUCLEIC ACIDS |
| EP2761662B1 (en) | 2011-09-30 | 2022-02-02 | Sony Group Corporation | Tungsten gates for non-planar transistors |
| JP6392857B2 (ja) | 2013-05-24 | 2018-09-19 | オッカム バイオラブス,インコーポレイティド | 核酸サンプルを回収するためのシステム及び方法 |
| US9644232B2 (en) * | 2013-07-26 | 2017-05-09 | General Electric Company | Method and device for collection and amplification of circulating nucleic acids |
| AU2014346562B2 (en) | 2013-11-07 | 2018-11-29 | The Board Of Trustees Of The Leland Stanford Junior University | Cell-free nucleic acids for the analysis of the human microbiome and components thereof |
| CN104894226A (zh) * | 2014-03-07 | 2015-09-09 | 复旦大学 | 检测实验室结核分枝杆菌微环境表面污染的方法及其检测试剂盒 |
| RU2729113C2 (ru) | 2014-11-21 | 2020-08-04 | Оккам Байолэбс, Инк. | Система и способ сбора образца нуклеиновой кислоты |
| JP6873921B2 (ja) | 2015-05-18 | 2021-05-19 | カリウス・インコーポレイテッド | 核酸の集団を濃縮するための組成物および方法 |
| ES2818569T3 (es) | 2015-09-09 | 2021-04-13 | Drawbridge Health Inc | Métodos para la recopilación, estabilización y conservación de muestras |
| EP3141612A1 (en) * | 2015-09-10 | 2017-03-15 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Method and device for nucleic acid based diagnostic approaches including the determination of a deviant condtion, especially a health condition and/or pathogenic condition of a sample |
| WO2017077999A1 (ja) * | 2015-11-06 | 2017-05-11 | 公立大学法人横浜市立大学 | 結核菌群由来dnaの検出方法 |
| CN105296661A (zh) * | 2015-12-02 | 2016-02-03 | 北京市结核病胸部肿瘤研究所 | 通过检测游离核酸诊断结核病的试剂盒及其应用 |
| EP3978627A1 (en) | 2016-03-25 | 2022-04-06 | Karius, Inc. | Methods using synthetic nucleic acid spike-ins |
| US20180298435A1 (en) * | 2016-07-05 | 2018-10-18 | Hitachi, Ltd. | DNA Detection Method and Device Therefor |
| EP4545649A3 (en) | 2016-11-11 | 2025-06-04 | Bio-Rad Laboratories, Inc. | Methods for processing nucleic acid samples |
| EP3573756A1 (en) * | 2017-01-30 | 2019-12-04 | Safeguard Biosystems Holdings Ltd. | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
| WO2018191563A1 (en) | 2017-04-12 | 2018-10-18 | Karius, Inc. | Sample preparation methods, systems and compositions |
| CN108165560B (zh) * | 2017-12-01 | 2021-06-08 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| CN108165561B (zh) * | 2017-12-01 | 2021-06-18 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| CN108165563B (zh) * | 2017-12-01 | 2021-02-19 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| CN108165562B (zh) * | 2017-12-01 | 2021-06-08 | 北京蛋白质组研究中心 | 结核分枝杆菌H37Rv编码基因及其应用 |
| CA3082601A1 (en) | 2018-03-16 | 2019-09-19 | Karius, Inc. | Sample series to differentiate target nucleic acids from contaminant nucleic acids |
| EP4700161A3 (en) | 2018-11-21 | 2026-03-11 | Karius, Inc. | Direct-to-library methods, systems, and compositions |
| CN111876411A (zh) * | 2019-09-06 | 2020-11-03 | 深圳微伴生物有限公司 | 获得cfDNA标准品的引物组、PCR扩增阳性标准品及其制备方法、试剂盒及应用 |
| KR102286570B1 (ko) * | 2019-11-19 | 2021-08-05 | 대한민국 | 소변 시료를 이용하여 결핵 진단용 프라이머, 프로브, 키트 및 이들의 용도 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101307355A (zh) * | 2007-11-30 | 2008-11-19 | 亚洲基因科技股份有限公司 | 检测结核分支杆菌和非结核分支杆菌核酸扩增产物的试纸与方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0528306B1 (en) * | 1991-08-15 | 1999-11-17 | F. Hoffmann-La Roche Ag | Mycobacterium primer pair |
| US6458366B1 (en) * | 1995-09-01 | 2002-10-01 | Corixa Corporation | Compounds and methods for diagnosis of tuberculosis |
| EP0975807B1 (en) * | 1997-05-02 | 2006-09-27 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
| DE19752898A1 (de) * | 1997-11-28 | 1999-08-05 | Centeon Pharma Gmbh | Verfahren zum Nachweis hoher Vierenkonzentrationen im Blutplasma und/oder Blutserum mittels der Polymerasekettenreaktion |
| US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| TWI237695B (en) * | 1999-12-14 | 2005-08-11 | Joy Biomedical Corp | Helicobacter pylori antigens in blood |
| AU2002224556A1 (en) * | 2000-07-24 | 2002-02-05 | Inpharmatica Limited | Adhesion molecules |
| CN1388378A (zh) * | 2002-06-17 | 2003-01-01 | 四川大学 | 结核分枝杆菌诊断检测试剂 |
| WO2005075683A1 (en) * | 2004-02-03 | 2005-08-18 | Postech Foundation | High throughput device for performing continuous-flow reactions |
| EP2351857B1 (en) * | 2005-02-17 | 2014-10-29 | TrovaGene, Inc. | Compositions and methods for detecting pathogen specific nucleic acids in urine |
| EP1844164B1 (en) * | 2005-11-15 | 2010-07-14 | Genoid KFT | Method of detecting pathogens using molecular beacons |
| US8741565B2 (en) * | 2005-12-30 | 2014-06-03 | Honeywell International Inc. | Oligonucleotide microarray for identification of pathogens |
| US8029806B2 (en) * | 2008-04-16 | 2011-10-04 | Asiagen Corporation | Method for diagnosing of Mycobacterium tuberculosis |
| WO2010080691A1 (en) * | 2009-01-06 | 2010-07-15 | Qimin You | Cross priming amplification of target nucleic acids |
| EP2694680A4 (en) * | 2011-04-01 | 2014-09-10 | Occam Biolabs Inc | METHOD AND KITS FOR DETECTION OF CELL-FREE NURVIVAL-SPECIFIC NUCLEIC ACIDS |
-
2012
- 2012-04-02 EP EP12765867.2A patent/EP2694680A4/en not_active Withdrawn
- 2012-04-02 US US14/009,199 patent/US20140147851A1/en not_active Abandoned
- 2012-04-02 CN CN201811203829.6A patent/CN109280713A/zh active Pending
- 2012-04-02 AU AU2012236109A patent/AU2012236109A1/en not_active Abandoned
- 2012-04-02 CN CN201280026842.3A patent/CN103814139B/zh not_active Expired - Fee Related
- 2012-04-02 JP JP2014502902A patent/JP6430826B2/ja not_active Expired - Fee Related
- 2012-04-02 RU RU2013148806A patent/RU2644672C2/ru not_active IP Right Cessation
- 2012-04-02 WO PCT/US2012/031814 patent/WO2012135815A2/en not_active Ceased
-
2017
- 2017-05-09 AU AU2017203071A patent/AU2017203071A1/en not_active Abandoned
- 2017-07-31 US US15/664,074 patent/US20170369932A1/en not_active Abandoned
-
2018
- 2018-05-10 JP JP2018091537A patent/JP2018117645A/ja active Pending
-
2019
- 2019-07-05 AU AU2019204844A patent/AU2019204844A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101307355A (zh) * | 2007-11-30 | 2008-11-19 | 亚洲基因科技股份有限公司 | 检测结核分支杆菌和非结核分支杆菌核酸扩增产物的试纸与方法 |
Non-Patent Citations (3)
| Title |
|---|
| JOU, NT等: "Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis", 《JOURNAL OF CLINICAL MICROBIOLOGY》 * |
| ROSSETTI, MLR等: "Improvement of Mycobacterium tuberculosis detection in clinical samples using DNA purified by glass matrix", 《JOURNAL OF MICROBIOLOGICAL METHODS》 * |
| STADEN等: "Detection of Mycobacterium tuberculosis in serum samples using the polymerase chain reaction", 《JOURNAL OF INFECTION》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114480691A (zh) * | 2022-01-24 | 2022-05-13 | 广州迪澳基因科技有限公司 | 基于熔解曲线检测结核分枝杆菌复合菌群的方法及试剂盒 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012135815A3 (en) | 2012-12-27 |
| EP2694680A2 (en) | 2014-02-12 |
| WO2012135815A2 (en) | 2012-10-04 |
| JP6430826B2 (ja) | 2018-11-28 |
| JP2018117645A (ja) | 2018-08-02 |
| US20170369932A1 (en) | 2017-12-28 |
| RU2644672C2 (ru) | 2018-02-13 |
| JP2014510538A (ja) | 2014-05-01 |
| AU2017203071A1 (en) | 2017-06-01 |
| RU2013148806A (ru) | 2015-05-10 |
| US20140147851A1 (en) | 2014-05-29 |
| AU2012236109A1 (en) | 2013-11-21 |
| EP2694680A4 (en) | 2014-09-10 |
| CN103814139B (zh) | 2018-11-13 |
| AU2019204844A1 (en) | 2019-07-25 |
| CN103814139A (zh) | 2014-05-21 |
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