CN109247280B - Rapid counting plate for copepods egg granules - Google Patents
Rapid counting plate for copepods egg granules Download PDFInfo
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- CN109247280B CN109247280B CN201810927829.4A CN201810927829A CN109247280B CN 109247280 B CN109247280 B CN 109247280B CN 201810927829 A CN201810927829 A CN 201810927829A CN 109247280 B CN109247280 B CN 109247280B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
- A01K61/95—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination specially adapted for fish
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Abstract
The invention provides a rapid counting plate for copepod eggs, which comprises a base, wherein adsorption micropores are arrayed on the base, the bottoms of the adsorption micropores are connected with a water outlet pipe, a water inlet pipe is arranged on the side of the orifice of the adsorption micropores, and the water outlet pipe and the water inlet pipe are respectively connected with a micro electric pump arranged on the base through hoses. The counting plate can rapidly and accurately count the number of mature copepods, has no error and no damage to the eggs, reduces the difficulty of counting the mature copepods, and has high metering efficiency.
Description
Technical Field
The invention belongs to the technical field of marine biology and water tank breeding, and particularly relates to a rapid copepod egg counting plate.
Background
Copepods belonging to the phylum Arthropoda, Crustacea, and copepoda. Is a small crustacean, the body length is less than 3 mm, and the planktonic and parasitic lives of the campuses are distributed in the ocean, fresh water or brackish water. It is one of the initial feeds for most fish larvae, and therefore has great significance in food nets for aquatic ecosystems and aquaculture. Therefore, the generational biological research on copepods is a very important research topic, and the mature egg granules of most copepods species have a diameter of about 100 microns. In the experiments of researching the hatchability of eggs, related toxicology research, nutriology research and the like, the counting of the eggs has great operation difficulty due to the tiny size, the eggs are carefully sucked from a culture water body by using a rubber head suction pipe and are counted by microscopic examination, the efficiency is low, and errors are easy to occur. New counting devices or methods are urgently needed to address this problem.
Disclosure of Invention
The invention aims to provide a rapid counting plate for rapidly and accurately counting mature copepods, which has no error and no damage to the eggs, reduces the difficulty in counting mature copepods, and has high metering efficiency.
The technical scheme adopted by the invention for realizing the purpose is as follows: the rapid copepod egg counting plate comprises a base, adsorption micropores are arrayed on the base, the bottoms of the adsorption micropores are connected with water outlet pipes, water inlet pipes are arranged on the sides of orifices of the adsorption micropores, and the water outlet pipes and the water inlet pipes are respectively connected with a micro electric pump arranged on the base through hoses. The base is carved with a coordinate system corresponding to the adsorption micropores arranged in an array mode, micro negative pressure is formed by a micro electric pump to form adsorption force on single mature copepods, so that the mature copepods are quickly positioned in the adsorption micropores and are not threshed, the quantity of the mature copepods can be calculated only by calculating the quantity of the adsorption micropores arranged in a regular square matrix, the counting difficulty is simplified, the counting work efficiency is improved, redundant copepods can be collected to be adsorbed and counted on the base next time, personnel do not need to use a rubber head suction pipe to suck the mature copepods from the small center of a culture water body and count the mature copepods under a microscope, the counting speed of the mature copepods can be greatly accelerated by the design of the invention, the precision rate is high, no error exists, and the mature copepods are not damaged in counting.
Furthermore, a top cover matched with the base is connected to the side of the base through a hinge. The setting of top cap can play the guard action to adsorbing the micropore, prevents that impurity from getting into to adsorb the micropore and causing broken influence to the ovum grain, still can calculate required copepods ovum grain quantity through the coordinate on the base simultaneously, realizes the control of the copepods ovum grain quantity that the single absorption obtained through the shielding plate.
Further, set up the window that corresponds with the absorption hole of array arrangement on the base on the top cap, the cooperation is connected with slidable shielding plate on the window, and the shielding plate surface is equipped with the boss. The shielding plate can slide on the window, the quantity control of the adsorption micropores for adsorbing copepods eggs on the base at a time is realized, the quantity of the copepods eggs can be conveniently obtained according to actual requirements, the design of the bosses on the shielding plate can improve the operation convenience of personnel, and the personnel can conveniently control the shielding plate to slide relative to the window.
Furthermore, a micro sucker is arranged on the hole surface of the adsorption micropore, and an adsorption structure is arranged on the adsorption surface of the sucker in a surrounding mode. When utilizing and adsorbing the micropore to collect the mature ovum grain of copepoda, through miniature charge pump drive outlet pipe drainage, form the negative pressure in adsorbing the micropore, the ovum grain can be adsorbed by adsorbing the micropore fast, the negative pressure that produces simultaneously can lead to the ovum grain damaged, adsorb the ovum grain through setting up the sucking disc and combining the adsorption micropore, there is minimum clearance between the hole face that can realize adsorbed ovum grain and adsorption micropore, reduce the negative pressure that the outlet pipe produced, form little negative pressure and adsorb the ovum grain, the ovum grain damage has been avoided simultaneously to quick effectual adsorption ovum grain.
Furthermore, the adsorption structure is composed of two rectangular groove bodies which are arranged in parallel, and the head end and the tail end between the two groove bodies are connected with a communicating groove. Because the sucking disc is required to adhere the object to the sucking surface of the sucking disc to form a certain vacuum degree to realize the adsorption of the eggs, by arranging the adsorption structure, a certain water body can exist in the adsorption structure in the process of adsorbing the eggs, when the eggs are contacted with the sucker, the eggs can be adsorbed with the sucker due to the self weight of the eggs and the negative pressure generated by the water outlet pipe, and simultaneously, most of the water in the adsorption structure is quickly discharged, the pore pressure in the two groove bodies of the adsorption structure after the water body is discharged is also continuously reduced to reduce the vacuum degree in the groove, the residual water body in the groove can flow between the two groove bodies through the communicating groove to maintain the vacuum degree between the sucker and the egg grains at a lower state to form a micro-suction state, the vacuum degree in the sucking disc can be adjusted to control the adsorption state of the egg grains and the sucking disc, and the egg grains are quickly adsorbed and fixed on the base and quickly washed off by water flow after counting is completed.
Furthermore, a water collecting tank is connected to the lower portion of the base through a second hinge, and water permeable holes of 10-20 microns are formed in the surface of the water collecting tank. When the base adsorbs the copepods ovum and washes unnecessary ovum, usable second hinge makes the plane height that converges the basin be less than or make and converge the basin and apply on the base back and avoid unnecessary copepods ovum to fall to and converge the basin and exert an influence to ovum count precision, make the basin surface that converges and the base surface parallel and level collect the copepods ovum that is washed off by the inlet tube and utilize the hole of permeating water to filter partial unnecessary water simultaneously when collecting the copepods ovum that the count was accomplished through the second hinge.
Furthermore, the outer side of the collecting water tank is connected with a baffle plate, and the tail end of the collecting water tank is connected with a discharge spout. The arrangement of the baffle can avoid that the copepods eggs obtained by counting fall to the outer side of the water collection tank in the process of falling to the water collection tank to cause the reduction of counting accuracy, and the discharge spout can transfer the collected copepods eggs with the counting completion into the collection container from the discharge spout.
Furthermore, the aperture of the adsorption micropores is 50-100 microns. The pore diameter of the adsorption micropores is set to be corresponding to the particle diameters of the copepods egg granules and most of the copepods mature egg granules, so that only mature copepods mature egg granules are collected, and immature copepods egg granules with undersize particle diameters are not adsorbed by the adsorption micropores.
Furthermore, the base and the top cover are made of transparent glass or plastic. The counting plate is convenient for an operator to observe or check the eggs on the counting plate during the use process and when the mature eggs of copepods are adsorbed by the adsorption micropores.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, micro negative pressure is formed by using a micro electric pump to form adsorption force on single mature eggs of the copepods, so that the mature eggs of the copepods are quickly positioned in adsorption micropores and are not threshed, the quantity of the mature eggs of the copepods can be calculated only by calculating the quantity of the adsorption micropores arranged in a regular square matrix, the counting difficulty is simplified, the counting working efficiency is improved, redundant eggs of the copepods can be collected for carrying out adsorption counting on a next base, and people do not need to use a rubber head suction pipe to suck the mature eggs of the copepods from a small center in a culture water body and count the mature eggs by microscopic examination.
The rapid counting plate for copepod eggs, provided by the technical scheme, overcomes the defects of the prior art, and is reasonable in design and convenient to operate.
Drawings
FIG. 1 is a schematic view of a base of a rapid copepod egg counting plate according to the present invention;
FIG. 2 is a schematic top view of a rapid copepod egg counting plate according to the present invention;
FIG. 3 is a schematic view of the connection between the adsorption micropores and the water outlet pipe of the present protector;
FIG. 4 is a top view of the chuck of the present invention;
FIG. 5 is a schematic view of the structure of the water collection tank of the present invention;
FIG. 6 is a schematic view of a shielding plate structure according to the present invention.
Description of reference numerals: 1, a base; 2. a hinge; 3, a water inlet pipe; 4, a hose; 5, a micro electric pump; 6-adsorption micropores; 7, collecting a water tank; 701-a second hinge; 702-a baffle; 8-discharge spout; 9-top cover; 10-window; 11-a shielding plate; 11a to a boss; 12-a sucker; 12 a-an adsorption structure; 13 to a water outlet pipe.
Detailed Description
The invention is described in further detail below with reference to examples and figures:
example 1:
as shown in fig. 1 to 6, the rapid counting plate for copepods eggs comprises a base 1, adsorption micropores 6 are arrayed on the base 1, the bottoms of the adsorption micropores 6 are connected with water outlet pipes 13, water inlet pipes 3 are arranged on the sides of the orifices of the adsorption micropores 6, and the water outlet pipes 13 and the water inlet pipes 3 are respectively connected with a micro electric pump 5 arranged on the base 1 through hoses 4. A coordinate system corresponding to the adsorption micropores 6 arranged in an array is carved on the base 1, micro negative pressure formed by a micro electric pump 5 is utilized to form adsorption force on single mature eggs of the copepods, so that the mature eggs of the copepods are quickly positioned in the adsorption micropores 6 and are not threshed, the quantity of the mature eggs of the copepods can be calculated only by calculating the quantity of the adsorption micropores 6 arranged in an orderly square array, the counting difficulty is simplified, the counting working efficiency is improved, redundant eggs of the copepods can be collected and adsorbed and counted on the base 1 next time, personnel do not need to use a rubber head suction pipe to suck the mature eggs of the copepods from a small center in a culture water body and perform microscopic examination and counting, the counting speed of the mature eggs of the copepods can be greatly accelerated, the accuracy is high, no error exists, and the mature eggs of the copepods are not damaged in counting.
The side of the base 1 is connected with a top cover 9 which is matched with the base 1 through a hinge 2. The top cover 9 can protect the adsorption micropores, prevents impurities from entering the adsorption micropores 6 to cause crushing influence on the eggs, and meanwhile, the number of the copepods eggs required by coordinate calculation on the base 1 can be calculated, and the control of the number of the copepods eggs obtained by single adsorption can be realized through the baffle plate 11.
The top cover 9 is provided with windows 10 corresponding to the adsorption holes 6 arrayed on the base 1, the windows 10 are connected with a slidable baffle plate 11 in a matching manner, and the surface of the baffle plate 11 is provided with a boss 11 a. The shielding plate 11 can slide on the window 10, the quantity control of the adsorption micropores 6 for adsorbing the copepods eggs on the base 1 at a time is realized, the quantity of the copepods eggs can be conveniently obtained according to actual requirements, the design of the boss 11a on the shielding plate 11 can improve the operation convenience of personnel, and the personnel can conveniently control the shielding plate 11 to slide and control relative to the window 10.
The hole surface of the adsorption micropore 6 is provided with a miniature sucker 12, and the adsorption surface of the sucker 12 is provided with an adsorption structure 12a in a surrounding manner. When utilizing absorption micropore 6 to collect the mature egg of copepoda, drive outlet pipe 13 drainage through miniature charge pump 5, form the negative pressure in absorption micropore 6, the egg can be adsorbed by absorption micropore 6 fast, the negative pressure that produces simultaneously can lead to the egg damaged, adsorb the egg through setting up sucking disc 12 combination absorption micropore, there is minimum clearance between the hole face that can realize absorbent egg and absorption micropore 6, reduce the negative pressure that outlet pipe 13 produced, form the micro-negative pressure and adsorb the egg, the egg damaged has been avoided simultaneously to quick effectual absorption egg.
The adsorption structure 12a is composed of two rectangular groove bodies arranged in parallel, and a communicating groove is connected between the two groove bodies at the head end and the tail end. Because the sucking disc 12 needs to adhere the object to the sucking surface of the sucking disc 12 to form a certain vacuum degree to realize the adsorption of the eggs, by arranging the adsorption structure 12a, a certain water body exists in the adsorption structure 12a in the process of adsorbing the eggs, when the eggs contact with the sucker 12, the eggs can be adsorbed by the sucker 12 due to the self weight of the eggs and the negative pressure generated by the water outlet pipe 13, and simultaneously, most of the water in the adsorption structure 12a can be quickly discharged, the pore pressure in the two groove bodies of the adsorption structure 12a after the water body is discharged is also continuously reduced to reduce the vacuum degree in the groove, part of water body remained in the groove can flow between the two groove bodies through the communicating groove to ensure that the vacuum degree between the sucking disc 12 and the egg granules is maintained at a lower state to form a micro-suction state, can realize that the adsorption state of the egg granules and the sucking disc 12 is controlled without adjusting the vacuum degree in the sucking disc 12, namely, the egg granules are quickly adsorbed and fixed on the base 1 and quickly washed down by water flow after counting is finished.
The outer side of the water collecting tank 7 is connected with a baffle 702, and the tail end is connected with a discharge spout 8. The arrangement of the baffle 702 can avoid that the counted copepods eggs fall to the outer side of the water collecting tank 7 in the process of falling to the water collecting tank 7 to cause the counting accuracy to be reduced, and the discharge spout 8 can transfer the collected copepods eggs with the counted ones to the collecting container from the discharge spout 8.
The aperture of the adsorption micropores 6 is 50-100 micrometers, the specific aperture is selected according to the particle size of the required ovum, the aperture of the adsorption micropores 6 is set to correspond to the particle sizes of the copepods ovum and most of the copepods mature ovum, only the mature copepods mature ovum can be collected, and the immature copepods ovum adsorption micropores 6 with undersized particle sizes do not adsorb the mature copepods ovum.
The base 1 and the top cover 9 are made of transparent glass or plastic. The counting plate is convenient for an operator to observe or check the eggs on the counting plate during the use process and when the mature eggs of copepods are adsorbed by the adsorption micropores 6.
Example 2:
when the copepod egg particle rapid counting plate is actually used:
1) before counting, the baffle plate 11 on the top cover 9 can be manually adjusted according to experimental requirements to fix the number of the eggs, and the second hinge 701 is utilized to enable the plane height of the water collecting tank 7 to be lower than or enable the water collecting tank 7 to be pasted on the back of the base 1 to avoid the redundant copepods eggs from falling into the water collecting tank 7;
2) starting the micro electric pump 5 to drive the water outlet pipe 13 to discharge water, and forming micro negative pressure in the adsorption micropores 6;
3) transferring a water body containing the egg grains by using a beaker or a large-mouth pipette, pouring an adsorption micropore 6, adsorbing the egg grains by using negative pressure, keeping the existence of micro negative pressure, and quickly checking the adsorption rate of the micropore to the egg grains by using a magnifying glass;
4) washing the surface of the counting plate by micro water flow, and washing the eggs which are not adsorbed to the micropores;
5) and (3) converting an electrode of a micro electric pump 5, driving the water inlet pipe 3 to absorb water, flushing the adsorption micropores 6, flushing the eggs adsorbed on the adsorption micropores 6 away from the micropores, collecting the eggs in a water tank 7, and transferring the eggs to a collection container through a discharge spout 8.
Counting test:
and counting mature eggs of the copepods (experimental group) by adopting a manual counting method (control group) and the counting plate of the invention, recording the time spent by the two groups, and assigning a third party to test the counting accuracy and the crushing condition of the eggs.
The manual counting method comprises the following steps: an operator carefully sucks the culture water body by using a rubber head suction pipe and performs microscopic examination and counting;
method of use of counting plate of the invention reference example 2;
counting requirements: the number of the obtained eggs is 100, the particle size is about 60 micrometers, and the counting result is as follows:
control group: the time for obtaining 100 eggs is 22 minutes and 17 seconds, wherein the particle size of 5 eggs is less than 50 micrometers, and 3 eggs are damaged;
experimental groups: the time for obtaining 100 eggs is 5 minutes and 24 seconds, the particle size of the obtained eggs meets the requirement, and the damage of the eggs does not occur.
The pore diameter of the adsorption micropores 6 of the present invention is not limited to 50 to 70 micrometers, but may be 50 micrometers, 51 micrometers, 52 micrometers, 53 micrometers, 54 micrometers … …, 99 micrometers, or 100 micrometers. The pore diameter of the water permeable holes formed on the surface of the collecting water tank 7 is not limited to 10-20 micrometers, but can also be 10 micrometers, 11 micrometers, 12 micrometers, 13 micrometers, 14 micrometers, 15 micrometers, 16 micrometers, 17 micrometers, 18 micrometers, 19 micrometers or 20 micrometers.
The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (5)
1. The rapid counting plate for copepod eggs is characterized in that: the device comprises a base (1), adsorption micropores (6) are arrayed on the base (1), the bottoms of the adsorption micropores (6) are connected with a water outlet pipe (13), a water inlet pipe (3) is arranged on the side of the orifice of the adsorption micropores (6), and the water outlet pipe (13) and the water inlet pipe (3) are respectively connected with a micro electric pump (5) arranged on the base (1) through a hose (4); a top cover (9) matched with the base (1) is connected to the side of the base (1) through a hinge (2); the top cover (9) is provided with windows (10) corresponding to the adsorption micropores (6) arranged in an array on the base (1), the windows (10) are connected with a slidable shielding plate (11) in a matching manner, and the surface of the shielding plate (11) is provided with a boss (11 a); a micro sucker (12) is arranged on the hole surface of the adsorption micropore (6), and an adsorption structure (12 a) is arranged on the adsorption surface of the sucker (12) in a surrounding manner; the adsorption structure (12 a) is composed of two rectangular groove bodies which are arranged in parallel, and the head end and the tail end between the two groove bodies are connected with a communicating groove.
2. The rapid copepod egg count plate of claim 1 wherein: the water collecting device is characterized in that a water collecting tank (7) is connected to the lower portion of the base (1) through a second hinge (701), and water permeable holes of 10-20 microns are formed in the surface of the water collecting tank (7).
3. The rapid copepod egg count plate of claim 2 wherein: the outer side of the water collecting tank (7) is connected with a baffle (702), and the tail end of the water collecting tank is connected with a discharge spout (8).
4. The rapid copepod egg count plate of claim 1 wherein: the aperture of the adsorption micropores (6) is 50-100 microns.
5. The rapid copepod egg count plate of claim 1 wherein: the base (1) and the top cover (9) are made of transparent glass or plastic.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810927829.4A CN109247280B (en) | 2018-08-15 | 2018-08-15 | Rapid counting plate for copepods egg granules |
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| CN201810927829.4A CN109247280B (en) | 2018-08-15 | 2018-08-15 | Rapid counting plate for copepods egg granules |
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| CN109247280B true CN109247280B (en) | 2021-04-20 |
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| CN112119960A (en) * | 2020-08-17 | 2020-12-25 | 浙江海洋大学 | Method for counting eggs of acrossocheilus fasciatus |
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| JP2010213602A (en) * | 2009-03-16 | 2010-09-30 | Nagasaki Prefecture | Mixed feed for initial seedling of useful herbivorous shellfish, and method for feeding the same |
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