CN219279913U

CN219279913U - Lymph node cell extraction element

Info

Publication number: CN219279913U
Application number: CN202221823816.0U
Authority: CN
Inventors: 许波; 王岩石; 张倩; 徐龙; 鲍加琪; 安然
Original assignee: Hefei Tongmian Biotechnology Co ltd
Current assignee: Hefei Tongmian Biotechnology Co ltd
Priority date: 2022-07-14
Filing date: 2022-07-14
Publication date: 2023-06-30
Anticipated expiration: 2032-07-14

Abstract

The utility model discloses a lymph node cell extraction device, which comprises: the surface of the grinding table is made of a frosted material; the contact surface of the T-shaped grinding rod and the grinding table is made of a frosted material; and the filtering device is positioned at the side part of the grinding table. The T-shaped grinding rod and the grinding surface of the grinding table, which is made of the grinding material, can sufficiently grind the lymph node tissues, so that the extraction efficiency of lymphocytes is greatly improved, the operation is convenient, and the filtering device can rapidly and simply filter cell suspension to generate more uniform single cells. The utility model has simple structure and convenient use.

Description

Lymph node cell extraction element

Technical Field

The utility model relates to the technical field of medical equipment, in particular to a lymph node cell extraction device.

Background

Lymph nodes are oval or broad bean-shaped lymph tissue bodies of different sizes and are in gray red when fresh. Mainly comprises lymphatic tissue and lymphatic sinus, and is covered with dense connective tissue envelope, and has functions of filtering lymph fluid and participating in immune reaction: (1) filtering lymph liquid: the lymph nodes are located on the path of lymph reflux, when pathogens, foreign matters and other harmful components invade superficial connective tissues in the body, the harmful components easily enter capillary lymphatic vessels throughout the whole body along with tissue fluid, flow back to the lymph nodes along with lymph fluid, and in the lymph sinus, the harmful components in the gonorrhea are fully contacted with macrophages in the sinus due to slow lymph flow velocity, so that the harmful components are cleared or confined in the lymph nodes. (2) Involved in the immune response, the lymph nodes, upon receiving the antigenic stimulus from the body, are able to mount specific immune responses to the antigen, after which the antigen-specific memory cells generated can monitor re-invasion of these harmful components by lymphocyte recirculation.

In the prior art, because the lymph node is small in volume, a laboratory adopts a copper mesh for mechanical grinding when obtaining lymph node cells, specifically, a lymphocyte separation liquid is added into a culture dish, a nylon mesh with 200 meshes is placed on the culture dish, and then a syringe piston is used for lightly grinding lymph node tissues, so that dispersed single cells penetrate the nylon mesh and enter the lymphocyte separation liquid in the culture dish. However, this method is low in extraction efficiency and inconvenient to operate, and therefore, development of a lymph node cell extraction device is highly demanded.

Disclosure of Invention

Aiming at the problems in the technical background, the utility model aims to provide the lymph node cell extraction device, which sufficiently grinds lymph node tissues through the T-shaped grinding rod and the grinding table, greatly improves the extraction efficiency of lymphocytes, and can quickly and simply filter cell suspension to generate more uniform single cells.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a lymph node cell extraction device comprising:

the surface of the grinding table is made of a frosted material;

the contact surface of the T-shaped grinding rod and the grinding table is made of a frosted material;

and the filtering device is positioned at the side part of the grinding table.

Further, the grinding device also comprises a grinding chamber, and the grinding table is transversely arranged inside the grinding chamber.

Further, an inclined body cell collecting table is arranged at the bottom of the grinding chamber and is positioned below the grinding table and the filtering device.

According to the technical scheme, the device further comprises a cell collecting chamber, wherein the cell collecting chamber is positioned on one side of the grinding chamber, and the bottoms of the cell collecting chamber and the grinding chamber are communicated through a notch.

Further, the filter device is located above the notch portion.

Further, the filtering device comprises a filtering plate, and a plurality of filtering net openings are formed in the filtering plate.

Still further, a clamping table is arranged on the inner wall of the grinding chamber, and the filter plate is detachably arranged on the clamping table.

Further, the T-shaped grinding rod comprises a holding part and a grinding part, wherein the bottom surface of the grinding part is in contact with the grinding table.

Compared with the prior art, the utility model has the following advantages:

the utility model has simple structure and convenient use, the grinding surfaces of the T-shaped grinding rod and the grinding table can sufficiently grind the lymph node tissues, the extraction efficiency of the lymphocytes is greatly improved, the operation is convenient, and the filtering device can rapidly and simply filter the cell suspension to generate more uniform single cells.

Drawings

FIG. 1 is a schematic diagram showing the structure of a lymph node cell extraction device according to the present utility model.

FIG. 2 is a schematic diagram of a T-shaped polishing rod according to the present utility model.

Fig. 3 is a schematic structural diagram of an embodiment of the present utility model.

In the figure: 1. a grinding chamber; 2. a grinding table; 3. a T-shaped grinding rod; 4. a filtering device; 5. an italic cell collection station; 6. a cell collection chamber; 7. a notch portion; 8. a clamping table; 9. a holding part; 10. and a polishing section.

Detailed Description

The following describes specific embodiments of the present utility model in detail with reference to the drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the utility model, are not intended to limit the utility model.

As shown in fig. 1 to 3, a lymph node cell extraction apparatus includes:

a grinding table 2, the surface of which is made of a frosted material;

a T-shaped grinding rod 3, wherein the contact surface of the T-shaped grinding rod and the grinding table 2 is made of a frosted material;

and a filter device 4 positioned at the side of the grinding table 2.

The utility model has simple structure and convenient use, the grinding surfaces of the T-shaped grinding rod 3 and the grinding table 2 can sufficiently grind the lymph node tissues, the extraction efficiency of the lymphocytes is greatly improved, the operation is convenient, and the filtering device 4 can rapidly and simply filter cell suspension to obtain more uniform single cells.

As shown in fig. 1, in one embodiment of the present utility model:

the grinding machine further comprises a grinding chamber 1, and the grinding table 2 is transversely arranged inside the grinding chamber 1.

In this embodiment, an inclined cell collection table 5 is disposed at the bottom of the polishing chamber 1, and the inclined cell collection table 5 is located below the polishing table 2 and the filtering device 4.

The ground tissue cells are filtered by the filter device 4, so that the cell suspension can be filtered rapidly and simply, and the cells pass through the filter device 4 to enter the italic cell collection table 5, and unnecessary membrane fibers and the like are left on the filter device 4.

In still another embodiment of the present utility model, the apparatus further comprises a cell collection chamber 6, wherein the cell collection chamber 6 is located at one side of the polishing chamber 1, and the bottoms of the cell collection chamber and the polishing chamber are communicated through a notch 7.

In this embodiment, the filter device 4 is located above the notch 7.

The filtered single cells flow along the inclined italic cell collection table 5 and enter the cell collection chamber 6 via the notch 7, the inclined italic cell collection table 5 helping the cell suspension to accumulate, reducing losses. Specifically, to facilitate cell collection, an outlet may be provided at the bottom of the cell collection chamber 6 to facilitate collection of the milled single cells.

As shown in fig. 2, the T-shaped polishing rod 3 includes a holding portion 9 and a polishing portion 10, and the bottom surface of the polishing portion 10 is in contact with the polishing table 2.

The polishing section 10 has a large bottom surface, and increases the contact area with the polishing table 2, thereby greatly improving the polishing efficiency and the extraction efficiency of lymph node cells.

In one embodiment of the utility model:

the filtering device 4 comprises a filtering plate, and a plurality of filtering net openings are formed in the filtering plate.

As shown in fig. 3, in this embodiment, a clamping table 8 is provided on the inner wall of the grinding chamber 1, and the filter plate is detachably mounted on the clamping table 8. The filter plate is convenient to detach, and impurities such as membrane fibers and the like which are retained above the filter plate are convenient to clean after the filter plate is used.

The using method comprises the following steps: in the experiment, a movable T-shaped grinding rod 3 is firstly arranged at the side of a grinding chamber 1, then samples such as lymph nodes and the like are arranged on a grinding table 2, a proper amount of normal saline or phosphate buffer solution is dripped, then the samples are fully ground by using the T-shaped grinding rod 3, after grinding, the grinding table 2 is washed by using the normal saline or the phosphate buffer solution, a tilting device is used for filtering the grinding solution through a filtering device 4, and the filtered cell solution enters a cell collecting chamber 6 through an oblique cell collecting table 5 so as to collect lymphocytes.

The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made therein without departing from the spirit and scope of the utility model, which is defined by the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. A lymph node cell extraction device, comprising:

the surface of the grinding table is made of a frosted material;

a filter device located at a side of the polishing table; the filtering device comprises a filtering plate, and a plurality of filtering net openings are formed in the filtering plate;

the grinding machine also comprises a grinding chamber, wherein the grinding table is transversely arranged inside the grinding chamber.

2. The lymph node cell extraction device of claim 1, wherein an italic cell collection table is provided at the bottom of the grinding chamber, the italic cell collection table being located below the grinding table and the filtering device.

3. The lymph node cell extraction device of claim 2 further comprising a cell collection chamber, wherein the cell collection chamber is located on one side of the grinding chamber and the bottoms of the two are communicated by a notch.

4. A lymph node cell extraction device according to claim 3, wherein the filter means is located above the notch.

5. The lymph node cell extraction apparatus of claim 1, wherein a clamping table is provided on an inner wall of the grinding chamber, and the filter plate is detachably mounted on the clamping table.

6. The lymph node cell extraction apparatus of claim 1, wherein the T-shaped polishing rod comprises a holding part and a polishing part, and a bottom surface of the polishing part is in contact with a polishing table.