CN216208009U - Novel systematic cell staining equipment - Google Patents

Novel systematic cell staining equipment Download PDF

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Publication number
CN216208009U
CN216208009U CN202122725637.5U CN202122725637U CN216208009U CN 216208009 U CN216208009 U CN 216208009U CN 202122725637 U CN202122725637 U CN 202122725637U CN 216208009 U CN216208009 U CN 216208009U
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China
Prior art keywords
chassis
tank
seted
cell staining
magnetic head
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CN202122725637.5U
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Chinese (zh)
Inventor
邓亚光
邓江平
聂庚梁
李宴清
覃程陈
孙长胜
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Yichang Meiguangguigu Life Science & Technology Co ltd
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Yichang Meiguangguigu Life Science & Technology Co ltd
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Abstract

The utility model discloses novel systematic cell staining equipment, which relates to the technical field of cell staining and comprises a chassis, wherein a collecting tank is arranged on the upper surface of the chassis, a magnetic suction component is arranged above the collecting tank, a first washing tank is arranged on the upper surface of the chassis, a fixed liquid tank is arranged on the upper surface of the chassis, a red-green staining tank is arranged on the upper surface of the chassis, a second washing tank is arranged on the upper surface of the chassis, a DAPI staining tank is arranged on the upper surface of the chassis, a third washing tank is arranged on the upper surface of the chassis, and a release tank is arranged on the upper surface of the chassis. It can set up through the cooperation of chassis, collecting vat, magnetism subassembly, first sink, fixed cistern, red green dyeing tank, second sink, DAPI dyeing tank, third sink and release groove, utilizes the magnetism subassembly of inhaling that sets up, can make the even cover of scanning of magnetic path when magnetism subassembly is inhaled and is rotated, has reduced the scanning time, has improved dyeing efficiency.

Description

Novel systematic cell staining equipment
Technical Field
The utility model relates to the technical field of cell staining, in particular to novel systematic cell staining equipment.
Background
Cell staining is a discipline formed by combining cytology and chemistry, and is a method for qualitatively locating and semi-quantitatively analyzing various chemical substances in blood cells based on cell morphology by combining the principle of chemical reaction.
When the existing cell staining equipment is used, a small rod-shaped magnet or an electromagnet is required to be used for continuously carrying out covering scanning in a six-hole culture plate, the scanning coverage of the magnetic rod or the electromagnet is uneven, the scanning time is long, and the staining efficiency is reduced; therefore, a novel systematic cell staining device is provided to solve the problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to make up the defects of the prior art and provides novel eight-well plate cell staining equipment.
In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a novel systematized cell staining equipment, includes the chassis, the collecting vat has been seted up to the upper surface on chassis, the top of collecting vat is provided with magnetism and inhales the subassembly, first sink has been seted up to the upper surface on chassis, fixed cistern has been seted up to the upper surface on chassis, red green staining bath has been seted up to the upper surface on chassis, the second sink has been seted up to the upper surface on chassis, DAPI staining bath has been seted up to the upper surface on chassis, the third sink has been seted up to the upper surface on chassis, the release tank has been seted up to the upper surface on chassis.
Further, the collecting tank, the first washing tank, the fixed liquid tank, the red-green dyeing tank, the second washing tank, the DAPI dyeing tank, the third washing tank and the releasing tank are annularly arranged at equal intervals.
Furthermore, a rubber pad is fixedly connected to the bottom surface of the chassis.
Furthermore, the magnetic assembly comprises a magnetic head, a first placing groove is formed in the upper surface of the magnetic head, a limiting plate is fixedly connected to the outer surface of the magnetic head, and the length of the limiting plate is larger than the diameter of the collecting groove.
Furthermore, the top end of the magnetic head is fixedly connected with a connecting column, a second placing groove is formed in the connecting column, and the bottom end of the second placing groove is communicated with the top end of the first placing groove.
Furthermore, an external thread is formed in the upper portion of the outer surface of the connecting column, a threaded cap is arranged above the second placing groove, and the inner wall of the threaded cap is in threaded connection with the external thread.
Furthermore, the inner top wall of the screw cap is fixedly connected with an elastic pad, and the elastic pad is positioned inside the second placing groove.
Furthermore, the upper surface of the threaded cap is fixedly connected with a holding plate, and a group of anti-skid grains are arranged on the outer surface of the holding plate.
Compared with the prior art, this neotype eight-hole board cell staining is equipped and is possessed following beneficial effect:
1. according to the utility model, through the matching arrangement of the chassis, the collecting tank, the magnetic attraction component, the first washing tank, the fixed liquid tank, the red-green dyeing tank, the second washing tank, the DAPI dyeing tank, the third washing tank and the release tank, the friction force between the chassis and an external experiment table can be increased by using the arranged rubber pads, the chassis is prevented from sliding, and the magnetic attraction component can be used for uniformly covering the scanning of the magnetic block when the magnetic attraction component rotates, so that the scanning time is reduced, and the dyeing efficiency is improved.
2. According to the device, the holding plate, the magnetic head, the collecting tank, the second placing groove, the first placing groove and the limiting plate are used, after the strip-shaped magnetic blocks are placed in the second placing groove and the first placing groove, the holding plate is held tightly and rotated, the holding plate can drive the magnetic head to rotate in the collecting tank, magnetic particles in samples in the collecting tank can be rapidly adsorbed on the outer surface of the magnetic head, and therefore the dyeing efficiency of the device can be improved.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic view of the magnetic assembly of the present invention;
FIG. 3 is an exploded view of the magnetic assembly of the present invention;
FIG. 4 is a schematic view of the structure of the screw cap portion of the present invention.
In the figure: 1 chassis, 2 collecting tanks, 3 magnetism subassembly, 301 magnetic head, 302 first standing groove, 303 limiting plate, 304 spliced pole, 305 second standing groove, 306 external screw thread, 307 screw cap, 308 elastic pad, 309 grip block, 4 first washtrough, 5 fixed flume, 6 red and green dyeing tank, 7 second washtrough, 8DAPI dyeing tank, 9 third washtrough, 10 release tanks, 11 rubber pads.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the utility model.
This embodiment provides a neotype systematization cell staining equipment, and this cell staining equipment is used for cell staining analysis, inhales the subassembly through the magnetism that sets up, can make the even cover of scanning of magnetic path, has reduced the scanning time, has improved dyeing efficiency.
Referring to fig. 1-4, a novel systematized cell staining equipment, which comprises a chassis 1, collecting vat 2 has been seted up to chassis 1's upper surface, collecting vat 2's top is provided with magnetism and inhales subassembly 3, magnetism and inhales subassembly 3 and includes magnetic head 301, magnetic head 301's length slightly is less than collecting vat 2's diameter, first standing groove 302 has been seted up to magnetic head 301's upper surface, magnetic head 301's external fixed surface is connected with limiting plate 303, and limiting plate 303's length is greater than collecting vat 2's diameter, utilize the limiting plate 303 that sets up, can play limiting displacement to magnetic head 301, avoid magnetic head 301's bottom and collecting vat 2's inner bottom wall to contact, prevent to cause magnetic head 301 and collecting vat 2 to cause collision friction.
The top end of the magnetic head 301 is fixedly connected with a connecting column 304, a second placing groove 305 is formed in the connecting column 304, the bottom end of the second placing groove 305 is communicated with the top end of the first placing groove 302, and a space formed by the second placing groove 305 and the first placing groove 302 is used for placing the bar-shaped magnetic blocks.
Outer screw thread 306 has been seted up on the upper portion of spliced pole 304 surface, and the top of second standing groove 305 is equipped with screw cap 307, and screw cap 307's inner wall and outer screw thread 306 threaded connection, through the cooperation setting of outer screw thread 306 and screw cap 307, can be convenient for dismantle screw cap 307.
The elastic pad 308 is fixedly connected to the inner top wall of the threaded cap 307, the elastic pad 308 is located inside the second placing groove 305, the elastic pad 308 is made of sponge materials with high density, a fixing effect can be achieved on the magnetic block inside the second placing groove 305, and shaking of the magnetic block is avoided.
The upper surface fixedly connected with of screw cap 307 holds board 309, and a set of anti-skidding line has been seted up to the surface of holding board 309, through the board 309 that holds that sets up, can be convenient for the operating personnel to inhale subassembly 3 and remove.
The upper surface of the chassis 1 is provided with a first washing tank 4, the upper surface of the chassis 1 is provided with a fixed liquid tank 5, the upper surface of the chassis 1 is provided with a red-green dyeing tank 6, the upper surface of the chassis 1 is provided with a second washing tank 7, the upper surface of the chassis 1 is provided with a DAPI dyeing tank 8, the upper surface of the chassis 1 is provided with a third washing tank 9, and the upper surface of the chassis 1 is provided with a release tank 10.
The collecting tank 2, the first washing tank 4, the fixed liquid tank 5, the red-green dyeing tank 6, the second washing tank 7, the DAPI dyeing tank 8, the third washing tank 9 and the release tank 10 are annularly arranged at equal intervals, and the sizes of the collecting tank 2, the first washing tank 4, the fixed liquid tank 5, the red-green dyeing tank 6, the second washing tank 7, the DAPI dyeing tank 8, the third washing tank 9 and the release tank 10 are the same.
The bottom surface of the chassis 1 is fixedly connected with a rubber pad 11, and the friction force between the chassis 1 and an external experiment table can be increased by using the arranged rubber pad 11, so that the chassis 1 is prevented from sliding.
The working principle is as follows: when the device is used, firstly, the threaded cap 307 is rotated, the threaded cap 307 is arranged in a matching way through the threaded cap 307 and the external thread 306, the threaded cap 307 moves upwards and is separated from the top end of the connecting column 304, then, the magnetic block is placed inside the second placing groove 305 and the first placing groove 302, then the threaded cap 307 is rotated to enable the threaded cap 307 to be connected with the connecting column 304 again, then, the holding plate 309 is gripped and rotated, the holding plate 309 is rotated to drive the threaded cap 307, the connecting column 304 and the magnetic head 301 to rotate, the magnetic head 301 rotates more than one circle inside the collecting tank 2 to absorb the magnetic particles in the sample inside the collecting tank 2, then the magnetic head 301 is moved into the first washing tank 4, the holding plate 309 is rotated lightly from left to right and moved up and down, the cleaning of the magnetic head 301 can be completed, irrelevant cells and impurities adsorbed on the outer surface of the magnetic head 301 are cleaned, then the magnetic head 301 is moved into the fixed liquid tank 5, moving the magnetic head 301 into a red-green-red dyeing tank 6 after several minutes to perform red-green dyeing on cells, completing red-green dyeing after thirty minutes, then moving the magnetic head 301 into a second washing tank 7, placing PBS (phosphate buffer solution) in the second washing tank 7 for cleaning residual dyeing liquid, then moving the magnetic head 301 into a DAPI dyeing tank 8 for performing DAPI dyeing on the cells for five minutes, completing DAPI dyeing after five minutes, then moving the magnetic head 301 into a third washing tank 9, placing PBS (phosphate buffer solution) in the third washing tank 9 for cleaning residual dyeing liquid on the outer surface of the magnetic head 301, moving the magnetic head 301 into a release tank 10 after cleaning, placing PBS (phosphate buffer solution) in the release tank 10, releasing the cells wrapped by the magnetic particles on the outer surface of the magnetic head 301 into the solution, and directly observing and analyzing the cells in a microscope, therefore, the magnetic blocks can be uniformly covered during scanning, the scanning time is shortened, and the dyeing efficiency is improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (8)

1. A novel systematized cell staining equipment comprises a chassis (1), and is characterized in that: collecting vat (2) have been seted up to the upper surface on chassis (1), the top of collecting vat (2) is provided with magnetism and inhales subassembly (3), first wash tank (4) have been seted up to the upper surface on chassis (1), fixed cistern (5) have been seted up to the upper surface on chassis (1), red green dyeing vessel (6) have been seted up to the upper surface on chassis (1), second wash tank (7) have been seted up to the upper surface on chassis (1), DAPI dyeing vessel (8) have been seted up to the upper surface on chassis (1), third wash tank (9) have been seted up to the upper surface on chassis (1), release tank (10) have been seted up to the upper surface on chassis (1).
2. The novel systematic cell staining apparatus of claim 1, wherein: the collecting tank (2), the first washing tank (4), the fixed liquid tank (5), the red-green dyeing tank (6), the second washing tank (7), the DAPI dyeing tank (8), the third washing tank (9) and the release tank (10) are annularly arranged at equal intervals.
3. The novel systematic cell staining apparatus of claim 1, wherein: the bottom surface of the chassis (1) is fixedly connected with a rubber pad (11).
4. The novel systematic cell staining apparatus of claim 1, wherein: subassembly (3) are inhaled to magnetism includes magnetic head (301), first standing groove (302) have been seted up to the upper surface of magnetic head (301), the surface fixedly connected with limiting plate (303) of magnetic head (301), just the length of limiting plate (303) is greater than the diameter of collecting vat (2).
5. The novel systematic cell staining apparatus of claim 4, wherein: the top end of the magnetic head (301) is fixedly connected with a connecting column (304), a second placing groove (305) is formed in the connecting column (304), and the bottom end of the second placing groove (305) is communicated with the top end of the first placing groove (302).
6. The novel systematic cell staining apparatus of claim 5, wherein: the upper part of the outer surface of the connecting column (304) is provided with an external thread (306), a threaded cap (307) is arranged above the second placing groove (305), and the inner wall of the threaded cap (307) is in threaded connection with the external thread (306).
7. The novel systematic cell staining apparatus of claim 6, wherein: an elastic pad (308) is fixedly connected to the inner top wall of the threaded cap (307), and the elastic pad (308) is located inside the second placing groove (305).
8. The novel systematic cell staining apparatus of claim 7, wherein: the upper surface of the threaded cap (307) is fixedly connected with a holding plate (309), and a group of anti-skidding threads are formed in the outer surface of the holding plate (309).
CN202122725637.5U 2021-11-08 2021-11-08 Novel systematic cell staining equipment Active CN216208009U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122725637.5U CN216208009U (en) 2021-11-08 2021-11-08 Novel systematic cell staining equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122725637.5U CN216208009U (en) 2021-11-08 2021-11-08 Novel systematic cell staining equipment

Publications (1)

Publication Number Publication Date
CN216208009U true CN216208009U (en) 2022-04-05

Family

ID=80906131

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202122725637.5U Active CN216208009U (en) 2021-11-08 2021-11-08 Novel systematic cell staining equipment

Country Status (1)

Country Link
CN (1) CN216208009U (en)

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