CN103954545A - Special filter membrane surface cell counting plate for detecting circulating tumor cells - Google Patents
Special filter membrane surface cell counting plate for detecting circulating tumor cells Download PDFInfo
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- CN103954545A CN103954545A CN201410207610.9A CN201410207610A CN103954545A CN 103954545 A CN103954545 A CN 103954545A CN 201410207610 A CN201410207610 A CN 201410207610A CN 103954545 A CN103954545 A CN 103954545A
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- filter membrane
- tally
- counting plate
- glass slide
- circulating tumor
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Abstract
The invention provides a special filter membrane surface cell counting plate for detecting circulating tumor cells. The special filter membrane surface cell counting plate comprises a glass slide, wherein a filter membrane placing region is mounted on the surface of the glass slide; a counting plate formed by square cells is arranged at one side of the filter membrane placing region above the glass slide; the sizes of the counting plate and the glass slide are adapted to that of a glass slide placing region. After being repeatedly tested and used, the special filter membrane surface cell counting plate has the advantages that the circular filter membrane placing region is arranged on the glass slide for placing a filter membrane; the glass slide is placed in the glass slide placing region of the counting plate; the position of the filter membrane corresponds to a counting region of the counting plate; the counting is very accurate; each straight line has codes, cell counting can be corrected to a specific coordinate position and region division can also be carried out; the counting accuracy is improved and the operation is convenient; the special filter membrane surface cell counting plate is good for recording and analyzing; the working efficiency is improved and the practicability is strong; the special filter membrane surface cell counting plate is suitable for general popularization and application.
Description
Technical field
The present invention relates to for the filter membrane detection method field of counting according to tumour cell size dimension, specifically a kind of New Cycle tumour cell Special filtering membrane superficial cell tally.
Background technology
The present invention is based on state key fundamental research development plan (973 plan) project, be numbered developing of 2011CB504302 and improve membrane filtration circulating tumor cell detection system, this system requirements adopts the filter membrane that the diameter of the filter opening that contains 8 microns is 15mm, after blood filtration screening, circulating tumor cell is retained in filter membrane surface, carry out accurate metering, and usually because filter membrane surface causes repeat count without object of reference, the present situations such as error of omission, cause testing result inaccurate, the circulating tumor cell detecting is carried out to location difficulty again, and cause the repeated authentication of result difficult, also there is no at present the equipment of counting for filter membrane superficial cell specially, make up the blank of this Related product, develop a kind of circulating tumor cell and detect Special filtering membrane superficial cell tally.
Summary of the invention
The object of this invention is to provide a kind of circulating tumor cell and detect Special filtering membrane superficial cell tally, it is calculated accurately, be convenient to record analysis.
For achieving the above object, the present invention by the following technical solutions, the invention provides circulating tumor cell Special filtering membrane superficial cell tally, comprises microslide, and described slide surface is installed filter membrane put area; The tally that microslide top and the one side setting of filter membrane rest area are comprised of grid; The slide rest area that tally and microslide size adapt to.
Above-mentioned circulating tumor cell detects Special filtering membrane superficial cell tally, and described tally is comprised of crisscross 1 * 1mm grid matrix, and reference numbers and alphabetic flag.
Above-mentioned circulating tumor cell detects Special filtering membrane superficial cell tally, and tally and microslide plane are wholely set.
Above-mentioned circulating tumor cell detects Special filtering membrane superficial cell tally, and tally also can arrange in the groove of microslide.
The present invention uses and knows there is following advantage through repetition test: on the microslide of placing filter membrane, have circular filter membrane put area, microslide is positioned in the slide rest area of tally, filter membrane position is corresponding with the count block of tally, counts more accurate; Every straight line has coding, and cell count can be as accurate as concrete coordinate position, can also carry out Region Segmentation, improves counting accuracy, easy to operate, is beneficial to record analysis, increases work efficiency, practical, suitablely generally applies.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further described.
Circulating tumor cell detects Special filtering membrane superficial cell tally, comprises microslide 1, and filter membrane put area 2 is installed on described microslide 1 surface; The tally 3 that microslide 1 top and the one side setting of filter membrane rest area are comprised of grid; The slide rest area that tally and microslide size adapt to.
Concrete circulating tumor cell detects Special filtering membrane superficial cell tally, and described tally is comprised of crisscross 1 * 1mm grid matrix, and reference numbers and alphabetic flag.
Concrete circulating tumor cell detects Special filtering membrane superficial cell tally, and tally and microslide plane are wholely set.
Concrete circulating tumor cell detects Special filtering membrane superficial cell tally, and tally also can arrange in the groove of microslide.
Using method: place under microscope filter membrane is observed to counting in the criss-cross method district on tally.Part not in the detailed description of the invention is known technology, and the common variation that those skilled in the art carries out within the scope of technical solution of the present invention or replacement all should be included in protection scope of the present invention.
Claims (4)
1. circulating tumor cell detects Special filtering membrane superficial cell tally, comprises microslide (1), it is characterized in that: filter membrane put area (2) is installed on described microslide (1) surface; The tally (3) that microslide (1) top and the one side setting of filter membrane rest area are comprised of grid; The slide rest area that tally and microslide size adapt to.
2. circulating tumor cell according to claim 1 detects Special filtering membrane superficial cell tally, it is characterized in that: described tally is comprised of crisscross 1 * 1mm grid matrix, and reference numbers and alphabetic flag.
3. circulating tumor cell according to claim 1 detects Special filtering membrane superficial cell tally, it is characterized in that: tally and microslide plane are wholely set.
4. circulating tumor cell according to claim 1 detects Special filtering membrane superficial cell tally, it is characterized in that: tally also can arrange in the groove of microslide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410207610.9A CN103954545A (en) | 2014-05-16 | 2014-05-16 | Special filter membrane surface cell counting plate for detecting circulating tumor cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410207610.9A CN103954545A (en) | 2014-05-16 | 2014-05-16 | Special filter membrane surface cell counting plate for detecting circulating tumor cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103954545A true CN103954545A (en) | 2014-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410207610.9A Pending CN103954545A (en) | 2014-05-16 | 2014-05-16 | Special filter membrane surface cell counting plate for detecting circulating tumor cells |
Country Status (1)
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| CN (1) | CN103954545A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247280A (en) * | 2018-08-15 | 2019-01-22 | 浙江海洋大学 | Copepods ovum grain fast counting panel |
| CN109738347A (en) * | 2019-02-25 | 2019-05-10 | 广州牛顿光学研究院有限公司 | A kind of cell count Special sample pond and production method |
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| CN2542672Y (en) * | 2001-07-10 | 2003-04-02 | 朝克 | Suspension cell counter |
| CN2676190Y (en) * | 2004-02-16 | 2005-02-02 | 复旦大学附属华山医院 | Urine deposit staining and quantitatively counting device |
| CN201080485Y (en) * | 2007-06-20 | 2008-07-02 | 深圳职业技术学院 | Cell culture container |
| CN101429234A (en) * | 2008-12-11 | 2009-05-13 | 中国人民解放军第四军医大学 | Vaccine based on simulating human blood vessel endothelial cell growth factor VEGF epitope and preparation method thereof |
| CN201387415Y (en) * | 2009-03-19 | 2010-01-20 | 农业部沼气科学研究所 | Cell counting plate |
| CN103725604A (en) * | 2014-01-08 | 2014-04-16 | 爱威科技股份有限公司 | Cell counting plate |
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2377532Y (en) * | 1999-06-10 | 2000-05-10 | 中国人民解放军第二军医大学 | Microfluorecyte counting plate |
| CN2542672Y (en) * | 2001-07-10 | 2003-04-02 | 朝克 | Suspension cell counter |
| CN2676190Y (en) * | 2004-02-16 | 2005-02-02 | 复旦大学附属华山医院 | Urine deposit staining and quantitatively counting device |
| CN201080485Y (en) * | 2007-06-20 | 2008-07-02 | 深圳职业技术学院 | Cell culture container |
| CN101429234A (en) * | 2008-12-11 | 2009-05-13 | 中国人民解放军第四军医大学 | Vaccine based on simulating human blood vessel endothelial cell growth factor VEGF epitope and preparation method thereof |
| CN201387415Y (en) * | 2009-03-19 | 2010-01-20 | 农业部沼气科学研究所 | Cell counting plate |
| CN103725604A (en) * | 2014-01-08 | 2014-04-16 | 爱威科技股份有限公司 | Cell counting plate |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247280A (en) * | 2018-08-15 | 2019-01-22 | 浙江海洋大学 | Copepods ovum grain fast counting panel |
| CN109247280B (en) * | 2018-08-15 | 2021-04-20 | 浙江海洋大学 | Rapid counting plate for copepods egg granules |
| CN109738347A (en) * | 2019-02-25 | 2019-05-10 | 广州牛顿光学研究院有限公司 | A kind of cell count Special sample pond and production method |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20161221 Address after: 255000 Shandong high tech Zone, Zibo, Shandong Road, No. 51, high polymer materials innovation park B block, room 2011 Applicant after: Shandong Weikang Medical Inspection Co. Ltd. Address before: 250014 Ji'nan Province, Lixia District, Shandong by Road No. ten, No. 18877 Applicant before: SHANDONG ACADEMY OF MEDICAL SCIENCES |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140730 |