CN2676190Y - Urine deposit staining and quantitatively counting device - Google Patents
Urine deposit staining and quantitatively counting device Download PDFInfo
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- CN2676190Y CN2676190Y CNU2004200201619U CN200420020161U CN2676190Y CN 2676190 Y CN2676190 Y CN 2676190Y CN U2004200201619 U CNU2004200201619 U CN U2004200201619U CN 200420020161 U CN200420020161 U CN 200420020161U CN 2676190 Y CN2676190 Y CN 2676190Y
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- arena
- calibration
- counting device
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Abstract
The utility model relates to a urine deposit staining and quantitatively counting device, comprising a glass stage staining plate and a counting calibration plate which is coupled with the glass stage staining plate whose plane is provided with a circular urine sample staining and counting pond and a pond wall whose circumference are colored, and the processing of preventing slice escaped from glass slide is carried out on the base of the pond. Circular big and small calibration zones are arranged on the counting calibration plate. The urine deposit staining and quantitatively counting device which has easy use and strong result reliability of the utility model conforms to the urine detecting standardized requirement established by the international (NCCLS) and the testing branch of the Chinese Medical Association, carrying out the histiocyte chemical counting of various components of the urine deposit and accurately quantitative damage detection, and the primitive data of a patient can be preserved for all time.
Description
Technical field:
The utility model belongs to medical instruments field, relates to a kind of urine verifying attachment, is specifically related to a kind of arena dyeing quantitative counting device.
Technical background:
The sediment urinalysis analysis is meant the particle that detects cast, various pathology senses in urine in the clinical urine check, as cell, bacterium, the inspection of crystallization, sperm, bacterium visible components such as (moulds) can be used for the monitoring of diagnosis and differential diagnosis, prognosis judgement and the drug therapy reaction of diseases such as urinary system.Be applied to clinically at present that the method for counting of visible component mainly contains in the urine, based on fluidic cell detection technique and two kinds of quantitative analysis methods of blood cell counting plate.The former is because the arena composition is too complicated, after doing fluorescent dye, the dyeing characteristic of its each cell component is subject to that multiple factor is disturbed and the degree of accuracy that has a strong impact on automatic counter system, be lower than 70% with clinical detection actual coincidence rate, and can not differentiate pathologic cast and abnormal cell, equipment price costliness in addition, technical sophistication, be difficult to popularize, still belong to a kind of clinical screening examination.Blood cell counting plate is on glass sheet wet sheet to be carried out the Direct observation counting.Its major technique defective is: 1. detect volume of urine and do not wait, each urine sample is too small, and the visible component skewness is not had corrective action, and the selection in the counting visual field has randomness; 2. be not suitable for dyeing; 3. the original sample data can't be preserved; 4. can not carry out qualitative and quantitative Accurate Analysis simultaneously, so its accuracy and repeated inequality and be difficult to standardization.
Summary of the invention:
The purpose of this utility model is for overcoming the defective of prior art, a kind of meet international and domestic uroscopy standardization are provided, can carry out histochemical stain, simultaneously qualitative and quantitative examination of urinary sediment device is specifically related to a kind of arena dyeing quantitative counting device.
The utility model is implemented by following technical proposals.
The utility model is made up of glass loading dyed plate 1 and counting calibration plate 6 two parts, wherein loading dyed plate and the coupling mutually of calibration plate.Described glass loading dyed plate plane is provided with circular urine sample dyeing counting pond 2, and interior warp is 8mm～12mm, outward through 8.1mm～12.2mm, and the high 0.10mm～0.25mm in pond, floorage is 50.26mm
2～113.10mm
2, pool wall 5 circumference are colour (red or blue), counting chamber space of a whole page sign 4 is A, and B, urine sample counting chamber bottom 3 usefulness poly-D-lysines and serum (10: 1) are prevented the flake processing; Counting calibration plate is provided with circular calibration district 7, and is identical with support board sample counting pond size.Calibration district is divided into the fan-shaped drift slide 8 of big lattice, and wherein to be divided into square little lattice be lattice drift slide 9 to each several part, and little lattice area is 0.1 * 0.1mm
2 Put cover glass 10 between loading dyed plate and the counting calibration plate.
Adopt the utility model to carry out urine sample dyeing quantitative counting, can be as follows and step,
1. go up sample: accurately draw fresh arena 50 μ l～150 μ l and splash in the sample counting pond drying at room temperature respectively.The abundant mixing of arena.
2. sample is fixed: select different immobile liquids that the sample of counting chamber bottom is fixed according to various colouring methods.
3. dyeing: the qualitative and quantitative counting of conventional sediment cell adopts Pasteur (Papanicolaou) dyeing, requires to give corresponding specific stain for special examined, as the immuno-enzymatic mark etc.Dyeing finishes to be checked with neutral gum cover glass mounting.
4. microscope qualitative examination: urinary sediment microscopy is identical with conventional microscope inspection checking method.
5. quantitative counting: adopt lattice drift slide (0.1 * 0.1 * 9) * 4mm usually
2Under low power lens, carry out quantitatively, distinguish (50.26mm when the remarkable skewness of arena visible component or the fan-shaped drift slide of the big lattice of the very few selection of quantity carry out circle calibration
2～113.10mm
2) inside counting.According to the particle number in the every microlitre urine of counting calculating.
6. take pictures with high explanation camera photography or digital camera and carry out the Computer Analysis counting, preserve sample mounting counting back.
The utility model one-time detection sample size can reach 150 μ l, is more than 100 times of conventional blood cell counting plate, can obviously improve the recall rate of pathological cells in the urine at microscopically, can reach 1/10
5More than.
The utlity model has following advantage:
1. arena sample volume and counting area obtain accurate quantification, and the position of each sample counting is identical, have guaranteed the accuracy of sample visible component counting, prevent the subjectivity of counting.
2. the subregion sign design of calibration plate count block can accurately be counted remarkable uneven visible component of Tissue distribution such as cast etc., effectively prevents the influence of sample distribution of particles inequality to counting.
3. the sample size in the loading sample counting pond is big and carry out the anticreep sheet to handle and suitable different histochemical stain, thereby significantly improves each paracytic recall rate.
4. the design of sample counting pond circle is identical with the shape that urine drips, and pool wall is consistent to the surface tension of sample.
5. because the arena sample volume obtains accurate quantification, realize in line with international standardsly, increased result's comparability.
6. circumference is that colour makes that the counting chamber area is clear debates, and can test to same sample with two identical sample countings of sheet pond, realizes own control, effectively prevents omission and false retrieval
7. can forever preserve after the original sample mounting also and can carry out repeat count, once use no cross pollution.
8. but both also available MICROCOMPUTER PROCESSING of manually-operated, cheap, technology is simple.The utility model arena dyeing quantitative counting device meets the uroscopy standardization requirement of the world (NCCLS) and the formulation of check branch of Chinese Medical Association, can carry out the histocytochemistry dyeing of various arena components, carry out qualitative simultaneously and the accurate quantification analysis, patient's source book is realized permanent the preservation.Easy to use, the credible result degree is strong, is fit to hospital application at different levels.
Description of drawings
Fig. 1 is the utility model glass loading dyed plate plan structure synoptic diagram,
Wherein, 1. glass loading dyed plate, 2. sample counting pond, 3. counting chamber bottom, 4. counting chamber sign.
Fig. 2 is the cross-sectional view of Fig. 1,
Wherein, 1. glass loading dyed plate, 5. pool wall.
Fig. 3 is the utility model counting calibration plate plan structure synoptic diagram
Wherein, 6. counting is calibrated plate, 7. circular calibration district, the 8. big fan-shaped drift slide of lattice, 9. lattice drift slide.
Fig. 4 is the utility model master TV structure synoptic diagram.
Wherein, 1. glass loading dyed plate, 5. pool wall, 6. counting calibration plate, 10 cover glasses
Embodiment
Embodiment 1: prepare the utility model device, carry out the dyeing quantitative counting of urine cast-off cells
Adopt transparency glass plate to prepare the loading dyed plate, two identical circular urine sample dyeing counting ponds are set on its plane, interior warp is 8.0mm, outward through 8.2mm, and high 0.1 5mm in pond, floorage is 50.26mm
2, the pool wall circumference is red, the flake processing is prevented with poly-D-lysine and serum (10: 1) in bottom, urine sample pond, adopts glass preparation counting calibration plate, and the circular district of calibrating is set on the plate, and is identical with support board sample counting pond size.Calibration district is divided into 4 fan-shaped drift slides of big lattice, and wherein to be divided into 9 little lattice of square be the lattice drift slide to each several part, and every little lattice area is 0.1 * 0.1mm
2
The fresh arena 50 μ l that draw through abundant mixing splash into respectively in the support board sample counting pond, after the drying at room temperature, arena is selected to fix in the mixed liquor of ether and 95% alcohol half and half and was inserted 80%, 70%, 50% alcohol in 15 minutes successively interior 30 seconds, placed distilled water then 2 minutes, and waited to do; With haematine dye liquor dyeing 15 minutes, water dashed; Watery hydrochloric acid with 0.5% removes haematine dye liquor unnecessary in the endochylema, and water dashes after the several seconds; Put in rare lithium carbonate solution and alkalized 1 minute, water dashes; With 95% dehydration of alcohol 2 minutes, water dashed; Dyeing with 95% alcohol washing 2 times, was put in the anhydrous alcohol and is dewatered after 3 minute in the EA dye liquor, put then in the dimethylbenzene and washed 2 times, with natural gum, cover glass sealing.Behind the conventional microscope qualitative examination, on cover glass, place the calibration tally and make calibration district's area and support board counting area overlapping fully, adopt lattice drift slide (0.1 * 0.1 * 9 * 4mm
2) the parallel digital photographing of counting staining cell under low power lens, then according to the cell number of the every microlitre of actual urine fluid samples volume calculation.Patient's arena staining section and digital image numbering are preserved.
Embodiment 2: the dyeing quantitative counting of cast in the urine
Adopt transparency glass plate to prepare the loading dyed plate, two identical circular urine sample dyeing counting ponds are set on its plane, interior warp is 12mm, outward through 12.2mm, and the high 0.25mm in pond, floorage is for being 113.10mm
2The pool wall circumference is red, because size of the cast volume in the arena and length all do not wait, it distributes uneven relatively, the fresh arena 150 μ l that draw through abundant mixing splash into respectively in the support board sample counting pond, and after the drying at room temperature, arena is fixed 15 minutes with 2.5% paraformaldehyde, water dashes, and waits to do; With the dyeing of the capable negativity of india ink 150 μ l, treat behind its bone dry at conventional test under microscope.Then on the sample counting pond, place the calibration tally and make calibration district's area and support board counting area overlapping fully, adopt big lattice fan type drift slide (28.28 * 4mm
2) the parallel digital photographing of the cast of counting in the arena under low power lens, then according to the cast number of the every microlitre of actual urine fluid samples volume calculation.Patient's arena staining section and digital image numbering are preserved.
Claims (8)
1. an arena dyeing quantitative counting device is characterized in that forming loading dyed plate and the coupling mutually of calibration plate by glass loading dyed plate and counting calibration plate two parts.
2. arena dyeing quantitative counting device according to claim 1, it is characterized in that described loading dyed plate plane is provided with 2 identical circular urine sample dyeing counting ponds, warp is 8mm～12mm in it, outward through 8.1mm～12.2mm, high 0.10mm～the 0.25mm in pond, floorage is 50.26mm
2～113.10mm
2
3. arena dyeing quantitative counting device according to claim 2 is characterized in that described counting chamber, and the pool wall circumference is colored.
4. arena dyeing quantitative counting device according to claim 2 is characterized in that preventing the flake processing bottom the described counting chamber.
5. arena dyeing quantitative counting device according to claim 1 is characterized in that described counting calibration plate is provided with and the identical circular calibration of support board sample counting pond size district.
6. arena dyeing quantitative counting device according to claim 5 is characterized in that described tally calibration district is divided into 4 fan-shaped drift slides of big lattice and 4 the little lattice calibration of square districts.
7. arena dyeing quantitative counting device according to claim 6 is characterized in that the every lattice in the little calibration of described tally district are 0.1 * 0.1 * 9mm
2
8. arena dyeing quantitative counting device according to claim 1 is characterized in that the one-time detection sample size is 50 μ l～150 μ l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2004200201619U CN2676190Y (en) | 2004-02-16 | 2004-02-16 | Urine deposit staining and quantitatively counting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2004200201619U CN2676190Y (en) | 2004-02-16 | 2004-02-16 | Urine deposit staining and quantitatively counting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2676190Y true CN2676190Y (en) | 2005-02-02 |
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ID=34483270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2004200201619U Expired - Fee Related CN2676190Y (en) | 2004-02-16 | 2004-02-16 | Urine deposit staining and quantitatively counting device |
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| Country | Link |
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| CN (1) | CN2676190Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103954545A (en) * | 2014-05-16 | 2014-07-30 | 山东省医学科学院 | Special filter membrane surface cell counting plate for detecting circulating tumor cells |
| CN106062557A (en) * | 2014-02-28 | 2016-10-26 | 希森美康株式会社 | Urine sample analysis method and reagent kit for urine sample analysis |
-
2004
- 2004-02-16 CN CNU2004200201619U patent/CN2676190Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106062557A (en) * | 2014-02-28 | 2016-10-26 | 希森美康株式会社 | Urine sample analysis method and reagent kit for urine sample analysis |
| CN106062557B (en) * | 2014-02-28 | 2021-01-15 | 希森美康株式会社 | Urine sample analysis method and urine sample analysis kit |
| CN103954545A (en) * | 2014-05-16 | 2014-07-30 | 山东省医学科学院 | Special filter membrane surface cell counting plate for detecting circulating tumor cells |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |