CN201266164Y - Device for counting urine cell tittle dyeing - Google Patents
Device for counting urine cell tittle dyeing Download PDFInfo
- Publication number
- CN201266164Y CN201266164Y CNU2008201578729U CN200820157872U CN201266164Y CN 201266164 Y CN201266164 Y CN 201266164Y CN U2008201578729 U CNU2008201578729 U CN U2008201578729U CN 200820157872 U CN200820157872 U CN 200820157872U CN 201266164 Y CN201266164 Y CN 201266164Y
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- counting
- overflow
- cover plate
- dyeing
- pool wall
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Abstract
The utility model relates to a counting device for micro staining quantifying of urine cast-off cells, comprising a carrying plate and a cover plate. The counting device is characterized in that the carrying plate is provided with an anti-overflow pool surrounded by anti-overflow pool walls, and a counting pool surrounded by counting pool walls is arranged in the anti-overflow pool, wherein the height of the counting pool walls is 0.1-0.2 mm lower than the height of the anti-overflow pool wall, and the cover plate can cover the anti-overflow pool. The counting device can realize micro staining during the process of complete staining without cross contamination, and has reliable result and lower cost.
Description
Technical field
The utility model relates to medical diagnosis device, belongs to medical diagnosis utensil technology, and particularly, the utility model relates to a kind of quantitative counting assembly of cast-off cells trace dyeing that is used to urinate, and can realize trace dyeing in the full dyeing process.
Background technology
The inspection of sediment urinalysis is routine clinical inspection item, is mainly used in the monitoring of diagnosis and differential diagnosis, prognosis judgement and the drug therapy reaction of disease in the urological system.Wherein, arena includes the particle of various casts and pathology sense, mainly is to come off and the cell that oozes out, also comprises visible components such as bacterium, crystal (as, medicine) crystallization, mould, cellulose, mucus.The unevenness of arena composition and each component particle show the difference of tension force, and meeting appreciable impact arena composition is in the homogeneity of microslide upper berth sheet.For example, cell is easy to overlapping, is difficult to form cell monolayer, thereby disturbs dyeing quality and microscopical qualitative and quantitative counting inspection.There is no at present and carry out the quantitatively special-purpose counting assembly of urine cell of trace dyeing, the colouring method of arena adopts fluid cylinder formula multisample to dye simultaneously usually, and its main shortcoming has: can't avoid the cross pollution of dyeing; Dyeing liquor and cleaning fluid requirement are big; A large amount of coloured dye liquor processing cost height; Directly the discharging environmental pollution is serious; Can not support the skeptophylaxis histochemical stain.
Adopt single sample trace staining technique to dye and not only can prevent the cross pollution of sample, but also significantly reduce the consumption of dyeing liquor, thereby realize the skeptophylaxis histochemical stain.But when the dyeing liquor trace, when for example dyeing liquor was less than 100ul, because the capillary influence of arena each component, dyeing liquor usually skewness caused the dyeing of sample significantly uneven, thereby causes the dyeing failure.Be the influence of the surface tension that prevents the arena each component to trace dyeing, long-felt by the inventor, the utility model adopts the design of cover plate formula, the volume and the height in counting chamber and anti-overflow pond have been regulated, made up a kind of quantitative counting assembly of cast-off cells trace dyeing that is used to urinate, overcome capillary influence, thereby can in the full dyeing process, realize trace dyeing.
Summary of the invention
Problem to be solved in the utility model is to overcome the deficiencies in the prior art, and a kind of quantitative counting assembly of cast-off cells trace dyeing that is used to urinate is provided, and can realize trace dyeing in the full dyeing process.Particularly, the purpose of this utility model is to provide a kind of quantitative counting assembly of cast-off cells trace dyeing that is used to urinate, it comprises support board 1 and cover plate 4, wherein, the anti-overflow pond 2 that useful anti-overflow pool wall 21 surrounds on the support board 1, and the counting chamber 3 that useful counting pool wall 31 surrounds in the anti-overflow pond 2, wherein count the aspect ratio of pool wall 31 and the height of anti-overflow pool wall 21 and hang down 0.1～0.2 millimeter, and cover plate 4 can cover anti-overflow pond 2.It is poor that counting pool wall 31 and anti-overflow pool wall 21 have certain altitude in a small amount like this, the adverse effect that surface tension produced of the liquid that adds in the counting chamber 3, can eliminate by covering cover plate 4, and excess liquid is flowed in the anti-overflow pond 2 but not pollute outside the support board 1.
In above-mentioned counting assembly, preferred counting chamber 3 is circular.Like this, counting chamber 3 can adapt to microscopical circular field of view.
In above-mentioned counting assembly, the interior diameter of preferred counting chamber 3 is 2.5～5.0 millimeters, and the height of counting pool wall 31 is 0.1 millimeter.Like this, counting chamber 3 can hold 0.49～1.96 cubic millimeter liquid.Once dyeing can reach 5～10 microlitres.Because counting pool wall 31 has certain thickness, therefore also the overall diameter of preferred counting chamber 3 is 3.0～5.5 millimeters in addition.In above-mentioned counting assembly, anti-overflow pond 2 can be the Any shape that can contain counting chamber 3, and preferred anti-overflow pond 2 is square.The interior length of side in preferred anti-overflow pond 2 is 5.0～10.0 millimeters, and the height of preferred anti-overflow pool wall 21 is 0.2 millimeter.Because anti-overflow pool wall 21 has certain thickness, therefore also the outer length of side in preferred anti-overflow pond 2 is 5.2～10.2 millimeters in addition.
In above-mentioned counting assembly, cover plate 4 can be the Any shape that can cover anti-overflow pond 2, and preferred cover plate 4 is a square, and its length of side is greater than the outer length of side in anti-overflow pond 2, and the length of side of especially preferred cover plate 4 is 12cm.In addition, cover plate 4 has certain thickness, as 0.5 millimeter.Take for the convenience of the users, preferably have on the cover plate 4 and hold brightly 42, hold bright 42 ends and preferably have sphere and hold a little 41.For example, holding bright 42 length can be 3～8 centimetres, and the sphere of its end holds a little that 41 diameter is 1～2 centimetre.
In above-mentioned counting assembly, support board 1 and cover plate 4 are normally made with transparent material, and optionally material for example has glass, plastics or other macromolecule transparent materials.Preferred support board 1 and cover plate 4 are all made by glass.
In addition, can also comprise counting calibration plate in the above-mentioned counting assembly, one and the identical calibration of size district, support board sample counting pond, especially circular calibration district are arranged on this counting calibration plate.For example, circular calibration district can be divided into 4 fan-shaped scaling blocks of big lattice, and wherein every part comprises 9 0.1 * 0.1 square millimeter square little lattice, and totally 36 little lattice of square are with handled easily person calibration technique.
The counting assembly that the utility model provides has following multiple beneficial effect:
1. micro-dyeing liquor can significantly reduce the consumption of dyeing liquor, significantly reduces coloured dye liquor processing cost height; Directly the discharging environmental pollution is serious; Can support the skeptophylaxis histochemical stain.
2. adopt single sample trace staining technique to carry out trace dyeing, once use no cross pollution.
3. circular urine sample trace dyeing counting pond.As, it only is 4.9 square millimeters that the calibration area can be as small as; Simultaneously one square anti-overflow pond is arranged, guaranteed that like this dyeing liquor is unlikely to cause the failure of dyeing when excessive in urine sample counting chamber outside.
4. the difference in height in circular urine sample trace dyeing counting pond and its outer square anti-overflow pond is 0.1～0.2mm, adds the glass cover slide thereon, has guaranteed that like this dyeing liquor distribution covers the sample dyeing counting chamber fully.
5. trace dyeing urine cell sample volume and counting area obtain accurate quantification, the position of each sample counting is identical, discharged of the influence of other component of arena to the film-making of urine cell, make the distribution of urine cell more even, guarantee the accuracy of sample visible component counting, prevented the subjectivity of counting.
6. counting the count block of calibrating on the plate has the design of subregion sign, needn't count each cell one by one when counting is urinated total cellular score, only counts the efficient that the interior cell of scale can significantly improve monitoring.
7. the design of sample counting pond circle is identical with the shape that urine drips, and pool wall is consistent to the surface tension of sample.
8. because the arena sample volume obtains accurate quantification, meet the uroscopy standardization requirement that check branch of the world (NCCLS) and Chinese Medical Association formulates, the result can be report with the granule number in every milliliter, thereby realizes in line with international standardsly, has increased result's comparability.
9. but long preservation also can be carried out repeat count after the sample mounting.
10. except manually-operated, also be convenient to realize automation mechanized operation, and this counting assembly is simple in structure, cheap.
Description of drawings
Fig. 1 is the structural representation that is used for urinating the quantitative counting assembly support board 1 of cast-off cells trace dyeing of the present utility model.
Fig. 2 is the structural representation that is used for urinating the quantitative counting assembly cover plate 4 of cast-off cells trace dyeing of the present utility model.
Fig. 3 is the quantitative cross-sectional view of counting assembly when dying operation of cast-off cells trace dyeing that be used to urinate of the present utility model.
Embodiment
Below in conjunction with accompanying drawing formation of the present utility model is further described:
Accompanying drawing 1-3 is the structural representation that is used to urinate the quantitative counting assembly of cast-off cells trace dyeing of the present utility model.Be used to urinate the quantitative counting assembly of cast-off cells trace dyeing and comprise support board 1 and cover plate 4, wherein, the anti-overflow pond 2 that useful anti-overflow pool wall 21 surrounds on the support board 1, and the counting chamber 3 that useful counting pool wall 31 surrounds in the anti-overflow pond 2, wherein count the aspect ratio of pool wall 31 and the height of anti-overflow pool wall 21 and hang down 0.1～0.2 millimeter, and cover plate 4 can cover anti-overflow pond 2.Wherein, support board 1 and cover plate 4 are all made by glass; Counting chamber 3 is circular, and its interior diameter is 2.5～5.0 millimeters, and the height of counting pool wall 31 is 0.1 millimeter; The interior length of side in anti-overflow pond 2 is 5.0～10.0 millimeters, and the height of anti-overflow pool wall 21 is 0.2 millimeter; Cover plate 4 is a square, and its length of side is 12cm, and to preferably have length on it be that 3～8 centimetres hold is bright 42, and holding bright 42 ends, to preferably have diameter be that 1～2 centimetre sphere holds a little 41.
The use of this counting assembly is as follows:
Arena is accurately drawn the fresh urine cell dilution suspension 10 μ l through abundant mixing after layering is handled, join in the counting chamber 3, in drying at room temperature.Then, select different immobile liquid 20 μ l that the sample in the counting chamber 3 is fixed according to colouring method.
Then, adopt Pasteur (Papanicolaou) decoration method (also can require to carry out specific stain) to carry out the qualitative and quantitative counting of conventional sediment cell as immuno-enzymatic mark or immunohistochemical staining etc. according to clinical special examined.Detailed process is:
1) distilled water is joined left standstill drying at room temperature in the counting chamber 32 minutes;
2) add haematine dye liquor 10 μ l in counting chamber 3, cover cover plate 4, dyeing liquor is evenly distributed on counting chamber 3 and opens cover plate 4 after interior 15 minutes, with flowing water flushing counting chamber 3 and cover plate 4;
3) with unnecessary haematine dye liquor in 0.25% the watery hydrochloric acid 10 μ l removal counting chamber 3, wash with flowing water after the several seconds;
4) add rare lithium carbonate solution 10 μ l in counting chamber 3 and cover cover plate 4 alkalization one minute, the flowing water flushing;
5) to counting chamber 3 usefulness 95% alcohol, 20 μ l and cover cover plate 4 dehydration 2 minutes, the flowing water flushing;
6) counting chamber 3 is added EA liquid 10 μ l and cover cover plate 4 dyeing 2-3 minute, till the painted distinctness of endochylema;
7) to counting chamber 3 usefulness 95% alcohol, 20 μ l and cover cover plate 4 washing 2-3 time, remove too much dye liquor, add anhydrous alcohol 20 μ l again and cover cover plate 4 and dewater, put dimethylbenzene 10 μ l then and cover cover plate 4 washed twice;
8) counting chamber 3 is added neutral gum 10 μ l, and optional available conventional cover glass mounting;
9) examine under a microscope cell and differential count.
Instantiation and accompanying drawing are illustrated the utility model.Yet, should be understood that the instantiation that provides only is in order to illustrate that obviously those of ordinary skill in the art can make various corrections and change to the present invention within the scope of the invention according to detailed description.
Claims (8)
1. one kind is used to urinate the quantitative counting assembly of cast-off cells trace dyeing, it comprises support board (1) and cover plate (4), it is characterized in that, support board (1) is gone up the anti-overflow pond (2) that useful anti-overflow pool wall (21) surrounds, and the counting chamber (3) that useful counting pool wall (31) surrounds in anti-overflow pond (2), wherein count the aspect ratio of pool wall (31) and the height of anti-overflow pool wall (21) and hang down 0.1～0.2 millimeter, and cover plate (4) can cover anti-overflow pond (2).
2. counting assembly according to claim 1 is characterized in that, counting chamber (3) is circular.
3. counting assembly according to claim 2 is characterized in that, the interior diameter of counting chamber (3) is 2.5～5.0 millimeters, and the height of counting pool wall (31) is 0.1 millimeter.
4. counting assembly according to claim 1 is characterized in that, anti-overflow pond (2) are square.
5. counting assembly according to claim 4 is characterized in that, the interior length of side of anti-overflow pond (2) is 5.0～10.0 millimeters, and the height of anti-overflow pool wall (21) is 0.2 millimeter.
6. counting assembly according to claim 1 is characterized in that, cover plate (4) is a square, and its length of side is 12cm.
7. counting assembly according to claim 1 is characterized in that, holds bright (42) on the cover plate (4), and holding bright (42) end has sphere to hold point (41).
8. counting assembly according to claim 1 is characterized in that, support board (1) and cover plate (4) are all made by glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201578729U CN201266164Y (en) | 2008-12-25 | 2008-12-25 | Device for counting urine cell tittle dyeing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201578729U CN201266164Y (en) | 2008-12-25 | 2008-12-25 | Device for counting urine cell tittle dyeing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201266164Y true CN201266164Y (en) | 2009-07-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201578729U Expired - Fee Related CN201266164Y (en) | 2008-12-25 | 2008-12-25 | Device for counting urine cell tittle dyeing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201266164Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103398949A (en) * | 2013-07-30 | 2013-11-20 | 济南华天恒达科技有限公司 | Disposable counting chamber |
| CN103852576A (en) * | 2012-12-03 | 2014-06-11 | 科宝智慧医疗科技（上海）有限公司 | Container for liquid analysis |
-
2008
- 2008-12-25 CN CNU2008201578729U patent/CN201266164Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103852576A (en) * | 2012-12-03 | 2014-06-11 | 科宝智慧医疗科技（上海）有限公司 | Container for liquid analysis |
| CN103852576B (en) * | 2012-12-03 | 2017-11-28 | 科宝智慧医疗科技（上海）有限公司 | Container for fluid analysis |
| CN103398949A (en) * | 2013-07-30 | 2013-11-20 | 济南华天恒达科技有限公司 | Disposable counting chamber |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090701 Termination date: 20141225 |
|
| EXPY | Termination of patent right or utility model |