CN109239052A - Spacefarer's urine detection method based on liquid core waveguide Raman spectrum - Google Patents
Spacefarer's urine detection method based on liquid core waveguide Raman spectrum Download PDFInfo
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
Spacefarer's urine detection method based on liquid core waveguide Raman spectrum that the invention discloses a kind of, this method is realized in spacefarer's urine detection system based on liquid core waveguide Raman spectrum, and this method includes that test liquid continues sample introduction, the test of dynamic liquid core waveguide Raman, waste liquid real-time collecting, test end and Data Post, remaining waste five steps of post-processing.The invention has the advantages that using based on movement liquid core waveguide Raman spectrum detection method, it can be achieved that enhancing and relatively large sample to faint Raman signal statistical measurement so that more reliable to the composition detection of spacefarer's urine；DC electric field method is used in waste collection, meets the requirement under microgravity environment；In test finally, carrying out the cleaning of wick-containing using water purification, meet the requirement of environmentally-friendly sanitary.
Description
Technical field
The present invention relates to a kind of raman microspectroscopy detection methods more particularly to a kind of based on liquid core waveguide laser Raman spectroscopy
Microcell detection method, suitable for, to the daily monitoring of spacefarer's urine, belonging to field of photodetection under the microgravity environment of space station.
Background technique
Manned space flight refers to that the mankind drive spacecraft and are engaged in flying for the round-trip earth such as various science, test, research in space
Row activity.Mainly there are manned spaceship, space station, outer celestial body campsite etc. in the region of spacefarer's work.Wherein space station is most common
It is also longer workplace.In space station, the case where spacefarer is in the environment of long-term microgravity, body need with
When be monitored, to find its potential health effect and problem.
The health monitoring of spacefarer can choose many test samples in spacecraft.Wherein, to the monitoring of spacefarer's urine
Most important to be also easier to obtain, the substance of monitoring includes creatinine, albumen, urea etc..Such as the creatinine in urine is muscle in people
The product being metabolized in vivo, is mainly excreted by glomerular filtration.Every 20g muscle metabolism can produce 1mg creatinine, in meat food
When intake is stablized, the muscle metabolism of body and not big variation, the generation of creatinine will be more constant.Urea nitrogen and creatinine
Increase simultaneously, illustrates that serious damage occurs in kidney.
Currently, urine detection instrument must use different principles in detection urine when different substances, including pH value, than close
Method, acid-base index agent, enzyme process etc. also need a large amount of reagent etc. to support, and are unable to satisfy the needs that spacefarer oneself operates detection.For
This, needs to find suitable spacefarer and rapidly and efficiently convenient and fast detection method and designs corresponding detection device, to meet space flight
The disconnected demand of member's real-time health diagnosis.
Laser Raman spectroscopy method is a kind of high molecular convenient feasible method of detection, but because of creatinine, the egg in urine
The content of white, urea etc. is lower, and Raman signal is very weak, so if Raman spectroscopy is used for spacefarer's Urine monitoring, needs to solve
Certainly the problem of its weak output signal, in addition it is also necessary to consider the space flight of microgravity, sample introduction, raffinate recycling, compact, durable etc.
The demand of aspect.
Summary of the invention
For above demand, the purpose of the present invention is to provide a kind of, and the spacefarer based on liquid core waveguide Raman spectrum urinates
Liquid detection method can meet under the space environment requirement of microgravity, compact, durable etc., creatinine, albumen, urine in urine
The Raman signal of the micro substances such as element detects, and reflects that the real-time of Partial Physiological Indexes of health condition is examined to spacefarer to realize
It is disconnected.
The present invention is achieved like this:
Spacefarer's urine detection method proposed by the present invention based on liquid core waveguide Raman spectrum is based on liquid core waveguide
It is realized in spacefarer's urine detection system of Raman spectrum, the system is mainly by master controller, collection subsystem, spectrum subsystem
System and sample introduction subsystem composition；
Wherein, spectroscopy subsystems are responsible for Laser emission and Raman signal and are received, it is by liquid core waveguide pipe, window slide, aobvious
Speck mirror, dichroscope, interferometric filter, beam expanding lens, Ramar laser, Rayleigh optical filter, fibre-coupled mirrors, reception optical fiber,
Raman spectrometer, total reflective mirror and sealing composition；One end of liquid core waveguide pipe is bonded window slide, and the other end is bonded total reflective mirror, entirely
Anti- mirror is reinforced fixing by sealing；
Sample introduction subsystem is analyzed for that will test in liquid-like (note: spacefarer's urine) feeding spectroscopy subsystems；Into
Subsystem is made of hose, liquid inlet device, electric piston, Step motor, inflow pipe, inlet and sealing cover；Test fluid
Body sample imports liquid inlet device by inlet, and sealing cover is covered after the completion of importing；Inflow pipe is connected to liquid core waveguide pipe, hose
Inflow pipe is connected with liquid inlet device；Step motor can drive electric piston to translate in liquid inlet device, and test liquid-like is passed through connection
It connects hose and inflow pipe is slowly pushed into liquid core waveguide pipe；
Collection subsystem is used to collect test liquid-like under the microgravity environment of space station, prevents pollution environment；Collect son
System is made of DC power supply, electric cathode, enclosed globe shade, outflow tube, electric anode and exhausted bath box；Outflow tube and liquid core waveguide pipe connect
It is logical；Enclosed globe shade inner wall has electric cathode, and waste liquid tank inlet is electric anode；The positive and negative anodes of DC power supply connect with electric anode electricity cathode respectively
It connects, maintains the electric field between electric anode and electric cathode；Test liquid-like in liquid core waveguide pipe is by outflow tube, along jet axis
Injection under the action of electric field, is attracted to electric anode, into exhausted bath box to electric cathode to take negative electrical charge；
Ramar laser can emit from right to left the continuous laser beam of certain wavelength X along optical main axis, expand through beam expanding lens
After (note: obtained lasing beam diameter is matched with the entrance pupil of microcobjective), then narrow frequency raman laser is obtained after interferometric filter
Beam is subsequently passed through dichroscope, after microcobjective and by window slide, can focus to and test liquid-like in liquid core waveguide pipe
(note: spacefarer's urine), the Raman Forward scattering signal of focal spot excitation is along wick-containing axis (note: wick-containing axis and optics master
Axis is completely coincident) it transmits to the left, and constantly work as transmission to be accumulated and be reinforced with the molecular collision in test liquid-like
To after total reflective mirror, reflected and transmitted to the right along wick-containing axis, again constantly with test liquid-like in molecular collision, Raman scattering
Signal is further strengthened, and after sequentially passing through window slide and microcobjective, after dichroscope reflects, passes along optical axis is received
Defeated, the Raman diffused light after Rayleigh optical filter filters off the pump light that wavelength is λ is focused through fibre-coupled mirrors enters reception optical fiber,
Incoming Raman spectrometer is analyzed；
Master controller is used to give step machine for starting and closing DC power supply, Ramar laser and Raman spectrometer
Motor sends control instruction, is also used to be arranged Raman spectrometer running parameter, and the spectroscopic data for receiving Raman spectrometer carries out
Analysis；
Spacefarer's urine detection method proposed by the present invention based on liquid core waveguide Raman spectrum the following steps are included:
(1) test liquid continues sample introduction
Liquid-like (note: spacefarer's urine) will be tested, liquid inlet device is imported by inlet, sealing cover is covered after the completion of importing；
Master controller issues instruction starting DC power supply, then gives Step motor transmission control instruction, drives electric piston in liquid inlet device
In slowly to right translation, test liquid-like by hose and inflow pipe is slowly pushed into liquid core waveguide pipe；The sample introduction process
It is lasting to carry out, run through entire test process, until test terminates；
(2) dynamic liquid core waveguide Raman is tested
When testing liquid-like full of entire liquid core waveguide pipe, master controller issues instruction starting Ramar laser and Raman
Raman spectrometer running parameter is arranged in spectrometer；Ramar laser emits the continuous laser beam of certain wavelength X from right to left, warp
Beam expanding lens expands obtains narrow frequency raman laser beam after interferometric filter again, is subsequently passed through dichroscope, through microcobjective and leads to
It after crossing window slide, focuses in liquid core waveguide pipe and tests liquid-like (note: spacefarer's urine), before the Raman of focal spot excitation
Transmitted to the left to scattered signal along wick-containing axis, and constantly with test liquid-like in molecular collision, to be accumulated and be added
By force, it after being transferred to total reflective mirror, is reflected and is transmitted to the right along wick-containing axis, constantly touched again with the molecule in test liquid-like
It hitting, Raman scattering signal is further strengthened, after sequentially passing through window slide and microcobjective, after dichroscope reflects,
Along optical axis transmission is received, the Raman diffused light after Rayleigh optical filter filters off the pump light that wavelength is λ is focused through fibre-coupled mirrors
Into reception optical fiber, be passed to Raman spectrometer, Raman spectrum data is real-time transmitted to master controller by Raman spectrometer, with into
Dynamic liquid core waveguide Raman spectrum data is constantly acquired and is stored by the continuous progress of sample, master controller；
(3) waste liquid real-time collecting
During the test, sample introduction is constantly carrying out；Test liquid-like in liquid core waveguide pipe is by outflow tube, along spray
It penetrates axis to spray to electric cathode, to take negative electrical charge, under the action of electric field, electric anode is attracted to, into exhausted bath box；
(4) test end and Data Post
When electric piston moves to bottom of right side in liquid inlet device, without test liquid-like in entire liquid inlet device, at this time
Test terminates；Master controller issues instruction and closes Ramar laser, Raman spectrometer；Master controller is by the multiple groups dynamic fluid flow of acquisition
Core waveguide Raman spectrum data carries out counting cumulative and average treatment, reflection spacefarer's health condition is obtained, in its urine
Creatinine, albumen, urea the relevant raman spectral signal of content, and this signal data is contrasted with health indicator, from
And complete health evaluating；
(5) remaining waste post-processes
Master controller sends control instruction to Step motor, drives electric piston in liquid inlet device slowly to left, directly
Extremely arrive liquid inlet device leftmost position；Sealing cover is opened, clean water is imported into liquid inlet device by inlet, is covered after the completion of importing close
Capping；Master controller sends control instruction to Step motor, drives electric piston in liquid inlet device slowly to right translation, will be clean
Water is slowly pushed into liquid core waveguide pipe by hose and inflow pipe, while son is collected in remaining waste indentation in liquid core waveguide pipe
System is collected, until electric piston moves to bottom of right side in liquid inlet device and terminates, liquid core waveguide pipe only has clean water at this time
In the presence of master controller issues instruction, disconnects DC power supply.
The invention has the advantages that using the detection method based on movement liquid core waveguide Raman spectrum, it can be achieved that micro-
The statistical measurement of the enhancing of weak Raman signal and relatively large sample, so that more reliable to the composition detection of spacefarer's urine；?
DC electric field method is used in waste collection, meets the requirement under microgravity environment；In test finally, carrying out wick-containing using water purification
Cleaning, meet the requirement of environmentally-friendly sanitary.
Detailed description of the invention
Fig. 1 is present system structural schematic diagram, in figure: 1 --- DC power supply；2 --- electric cathode；3 --- enclosed globe shade；
4 --- jet axis；5 --- outflow tube；6 --- liquid core waveguide pipe；7 --- wick-containing axis；8 --- inflow pipe；9 --- window
Slide；10 --- microcobjective；11 --- optical main axis；12 --- dichroscope；13 --- interferometric filter；14 --- it expands
Mirror；15 --- Ramar laser；16 --- Rayleigh optical filter；17 --- fibre-coupled mirrors；18 --- reception optical fiber；19 --- it draws
Graceful spectrometer；20 --- hose；21 --- liquid inlet device；22 --- test liquid-like；23 --- electric piston；24 --- step
Electromechanical machine；25 --- master controller；26 --- total reflective mirror；27 --- sealing；28 --- electric anode；29 --- exhausted bath box；
30 --- collection subsystem；31 --- spectroscopy subsystems；32 --- sample introduction subsystem；33 --- receive optical axis；34 --- feed liquor
Mouthful；35 --- sealing cover.
Specific embodiment
The specific embodiment of the invention is as shown in Figure 1.
Spacefarer's urine detection method proposed by the present invention based on liquid core waveguide Raman spectrum is based on liquid core waveguide
It is realized in spacefarer's urine detection system of Raman spectrum, the system is mainly by master controller 25, collection subsystem 30, spectrum
Subsystem 31 and sample introduction subsystem 32 form；
Wherein, spectroscopy subsystems 31 are responsible for Laser emission and Raman signal receives, it is by liquid core waveguide pipe 6, window slide
9, microcobjective 10, dichroscope 12, interferometric filter 13, beam expanding lens 14, Ramar laser 15, Rayleigh optical filter 16, optical fiber
Coupling mirror 17, reception optical fiber 18,27 composition of Raman spectrometer 19, total reflective mirror 26 and sealing；One end of liquid core waveguide pipe 6 is bonded window
Mouth slide 9, the other end are bonded total reflective mirror 26, and total reflective mirror 26 is reinforced fixing by sealing 27；
Sample introduction subsystem 32 divides for that will test liquid-like 22 (note: spacefarer's urine) and be sent into spectroscopy subsystems 31
Analysis；Sample introduction subsystem 32 is by hose 20, liquid inlet device 21, electric piston 23, Step motor 24, inflow pipe 8,34 and of inlet
Sealing cover 35 forms；It tests liquid-like 22 and liquid inlet device 21 is imported by inlet 34, sealing cover 35 is covered after the completion of importing；It becomes a mandarin
Pipe 8 is connected to liquid core waveguide pipe 6, and inflow pipe 8 is connected by hose 20 with liquid inlet device 21；Step motor 24 can drive electronic work
Plug 23 translates in liquid inlet device 21, and test liquid-like 22 is slowly pushed into liquid core waveguide pipe by hose 20 and inflow pipe 8
6；
Collection subsystem 30 is used to collect test liquid-like 22 under the microgravity environment of space station, prevents pollution environment；It receives
Subsystem 30 is made of DC power supply 1, electric cathode 2, enclosed globe shade 3, outflow tube 5, electric anode 28 with exhausted bath box 29；Outflow tube 5
It is connected to liquid core waveguide pipe 6；3 inner wall of enclosed globe shade has electric cathode 2, and 29 entrance of exhausted bath box is electric anode 28；DC power supply 1 it is positive and negative
Pole is connect with the electric cathode 2 of electric anode 28 respectively, maintains the electric field between electric anode 28 and electric cathode 2；Survey in liquid core waveguide pipe 6
Liquid-like 22 is tried by outflow tube 5, is sprayed along jet axis 4 to electric cathode 2, so that negative electrical charge is taken, under the action of electric field,
It is attracted to electric anode 28, into exhausted bath box 29；
Ramar laser 15 can emit from right to left the continuous laser beam of certain wavelength X along optical main axis 11, through beam expanding lens
After 14 expand (note: obtained lasing beam diameter is matched with the entrance pupil of microcobjective 10), then obtain after interferometric filter 13 narrow
Frequency raman laser beam, is subsequently passed through dichroscope 12, after microcobjective 10 and by window slide 9, can focus to wick-containing wave
Liquid-like 22 (note: spacefarer's urine) is tested in conduit 6, the Raman Forward scattering signal of focal spot excitation is along wick-containing axis 7
(note: wick-containing axis 7 is completely coincident with optical main axis 11) transmits to the left, and constantly with test liquid-like 22 in molecular collision,
To being accumulated and be reinforced, after being transferred to total reflective mirror 26, reflected and transmitted to the right along wick-containing axis 7, again constantly with survey
The molecular collision in liquid-like 22 is tried, Raman scattering signal is further strengthened, and sequentially passes through window slide 9 and microcobjective
After 10, after the reflection of dichroscope 12, transmitted along optical axis 33 is received, after Rayleigh optical filter 16 filters off the pump light that wavelength is λ
Raman diffused light focused through fibre-coupled mirrors 17 and enter reception optical fiber 18, incoming Raman spectrometer 19 is analyzed；
Master controller 25 is used for for starting and closing DC power supply 1, Ramar laser 15 and Raman spectrometer 19
Control instruction is sent to Step motor 24, is also used to be arranged 19 running parameter of Raman spectrometer, and receive Raman spectrometer 19
Spectroscopic data is analyzed；
Spacefarer's urine detection method proposed by the present invention based on liquid core waveguide Raman spectrum the following steps are included:
(1) test liquid continues sample introduction
Test liquid-like 22 (note: spacefarer's urine) is passed through into inlet 34 and imports liquid inlet device 21, is covered after the completion of importing
Sealing cover 35；Master controller 25 issues instruction starting DC power supply 1 and then sends control instruction to Step motor 24, drives electricity
Piston 23, slowly to right translation, test liquid-like 22 is slowly pushed away by hose 20 and inflow pipe 8 in liquid inlet device 21
Enter liquid core waveguide pipe 6；The sample introduction process persistently carries out, and runs through entire test process, until test terminates；
(2) dynamic liquid core waveguide Raman is tested
When testing liquid-like 22 full of entire liquid core waveguide pipe 6, master controller 25 issues instruction starting Ramar laser
15 and Raman spectrometer 19,19 running parameter of Raman spectrometer is set；Ramar laser 15 emits certain wavelength X from right to left
Continuous laser beam expands through beam expanding lens 14 and obtains narrow frequency raman laser beam after interferometric filter 13 again, is subsequently passed through dichroic
Mirror 12 focuses to the test (note: spacefarer of liquid-like 22 in liquid core waveguide pipe 6 after microcobjective 10 and by window slide 9
Urine), focal spot excitation Raman Forward scattering signal transmitted to the left along wick-containing axis 7, and constantly with test liquid-like 22
In molecular collision after being transferred to total reflective mirror 26, reflected and transmitted to the right along wick-containing axis 7 to be accumulated and be reinforced,
Again constantly with the molecular collision in test liquid-like 22, Raman scattering signal is further strengthened, and sequentially passes through window glass
After piece 9 and microcobjective 10, after the reflection of dichroscope 12, is transmitted along optical axis 33 is received, filter off wavelength through Rayleigh optical filter 16
It is focused for the Raman diffused light after the pump light of λ through fibre-coupled mirrors 17 and enters reception optical fiber 18, be passed to Raman spectrometer 19, drawn
Raman spectrum data is real-time transmitted to master controller 25 by graceful spectrometer 19, and with the continuous progress of sample introduction, master controller 25 is not
Disconnected acquire dynamic liquid core waveguide Raman spectrum data stores；
(3) waste liquid real-time collecting
During the test, sample introduction is constantly carrying out；Test liquid-like 22 in liquid core waveguide pipe 6 passes through outflow tube 5,
It sprays along jet axis 4 to electric cathode 2 to take negative electrical charge and under the action of electric field, is attracted to electric anode 28, enter
Exhausted bath box 29；
(4) test end and Data Post
When electric piston 23 moves to bottom of right side in liquid inlet device 21, without test liquid-like in entire liquid inlet device 21
22, test terminates at this time；Master controller 25 issues instruction and closes Ramar laser 15, Raman spectrometer 19；Master controller 25 will
The multiple groups dynamic liquid core waveguide Raman spectrum data of acquisition carries out counting cumulative and average treatment, obtains reflection spacefarer's health feelings
Condition, raman spectral signal relevant to the creatinine in its urine, albumen, the content of urea, and by this signal data and be good for
Kang Zhibiao is contrasted, to complete health evaluating；
(5) remaining waste post-processes
Master controller 25 to Step motor 24 send control instruction, drive electric piston 23 in liquid inlet device 21 slowly to the left
Translation, until arriving 21 leftmost position of liquid inlet device；Sealing cover 35 is opened, clean water is imported into liquid inlet device 21 by inlet 34,
Sealing cover 35 is covered after the completion of importing；Master controller 25 to Step motor 24 send control instruction, drive electric piston 23 into
Slowly to right translation in liquid device 21, clean water is slowly pushed into liquid core waveguide pipe 6 by hose 20 and inflow pipe 8, simultaneously
Remaining waste indentation collection subsystem 30 in liquid core waveguide pipe 6 is collected, until electric piston 23 translates in liquid inlet device 21
Terminate to bottom of right side, liquid core waveguide pipe 6 only has clean water to exist at this time, and master controller 25 issues instruction, disconnects DC power supply
1。
Claims (1)
1. a kind of spacefarer's urine detection method based on liquid core waveguide Raman spectrum, this method is based on liquid core waveguide Raman
It is realized in spacefarer's urine detection system of spectrum, the system comprises master controllers (25), collection subsystem (30), spectrum
Subsystem (31) and sample introduction subsystem (32) composition；It is characterized in that the detection method comprises the following steps:
1) test liquid continues sample introduction
Liquid-like, i.e. spacefarer's urine will be tested, liquid inlet device is imported by inlet, sealing cover is covered after the completion of importing；Master control
Device processed issues instruction starting DC power supply, then gives Step motor transmission control instruction, and electric piston is driven to delay in liquid inlet device
Slowly to right translation, test liquid-like is slowly pushed into liquid core waveguide pipe by hose and inflow pipe；The sample introduction process continues
It carries out, runs through entire test process, until test terminates；
2) dynamic liquid core waveguide Raman is tested
When testing liquid-like full of entire liquid core waveguide pipe, master controller issues instruction starting Ramar laser and Raman spectrum
Raman spectrometer running parameter is arranged in instrument；Ramar laser emits the continuous laser beam of certain wavelength X from right to left, through expanding
Mirror expands obtains narrow frequency raman laser beam after interferometric filter again, is subsequently passed through dichroscope, through microcobjective and passes through window
It after mouth slide, focuses to and tests liquid-like in liquid core waveguide pipe, the Raman Forward scattering signal of focal spot excitation is along wick-containing axis
Line transmits to the left, and constantly with the molecular collision in test liquid-like, to be accumulated and be reinforced, when being transferred to total reflective mirror
Afterwards, it is reflected and is transmitted to the right along wick-containing axis, again constantly with the molecular collision in test liquid-like, Raman scattering signal obtained
It further strengthens, after sequentially passing through window slide and microcobjective, after dichroscope reflects, along optical axis transmission is received, through auspicious
Sharp optical filter, which filters off wavelength and focuses for the Raman diffused light after the pump light of λ through fibre-coupled mirrors, enters reception optical fiber, is passed to and draws
Raman spectrum data is real-time transmitted to master controller by graceful spectrometer, Raman spectrometer, with the continuous progress of sample introduction, main control
Dynamic liquid core waveguide Raman spectrum data is constantly acquired and is stored by device；
3) waste liquid real-time collecting
During the test, sample introduction is constantly carrying out；Test liquid-like in liquid core waveguide pipe is by outflow tube, along injection axis
Line is sprayed to electric cathode, to take negative electrical charge, under the action of electric field, electric anode is attracted to, into exhausted bath box；
4) test end and Data Post
When electric piston moves to bottom of right side in liquid inlet device, tested at this time without test liquid-like in entire liquid inlet device
Terminate；Master controller issues instruction and closes Ramar laser, Raman spectrometer；Master controller is by the multiple groups dynamic wick-containing wave of acquisition
It leads Raman spectrum data to carry out counting cumulative and average treatment, reflection spacefarer's health condition is obtained, with the flesh in its urine
Acid anhydride, albumen, urea the relevant raman spectral signal of content, and this signal data is contrasted with health indicator, thus complete
At health evaluating；
5) remaining waste post-processes
Master controller sends control instruction to Step motor, drives electric piston in liquid inlet device slowly to left, until arriving
Liquid inlet device leftmost position；Sealing cover is opened, clean water is imported into liquid inlet device by inlet, sealing is covered after the completion of importing
Lid；Master controller sends control instruction to Step motor, drives electric piston in liquid inlet device slowly to right translation, by clean water
It is slowly pushed into liquid core waveguide pipe by hose and inflow pipe, while subsystem is collected into remaining waste indentation in liquid core waveguide pipe
System is collected, until electric piston moves to bottom of right side in liquid inlet device and terminates, liquid core waveguide pipe only has clean water to deposit at this time
, master controller sending instruction, disconnection DC power supply.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110455777A (en) * | 2019-09-12 | 2019-11-15 | 中科院合肥技术创新工程院 | Creatinine detection method in Novel urine based on Surface enhanced Raman spectroscopy |
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