CN202101939U - Measuring device for aflatoxin - Google Patents
Measuring device for aflatoxin Download PDFInfo
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- CN202101939U CN202101939U CN2011201569914U CN201120156991U CN202101939U CN 202101939 U CN202101939 U CN 202101939U CN 2011201569914 U CN2011201569914 U CN 2011201569914U CN 201120156991 U CN201120156991 U CN 201120156991U CN 202101939 U CN202101939 U CN 202101939U
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- aflatoxin
- light source
- signal
- measurement mechanism
- sample
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Abstract
The utility model relates to a measuring device for aflatoxin. The measuring device for the aflatoxin is characterized by comprising an excitation light source, a sample detecting pool, a signal measuring and processing device and a reading device. The measuring device for the aflatoxin is characterized in that the excitation light source comprises a laser light source which can be used for transmitting laser with the wavelength of 375+/-5nm and conducting optical fibers, the signal measuring and processing device is formed by sequentially distributing a focusing lens, optical gratings, a photomultiplier tube and an analog-digital (A/D) converter, and the focusing lens is positioned in a direction vertical to the excitation light, so that a fluorescent signal generated by a sample in the sample detecting pool is focused by the focusing lens and then reaches the photomultiplier tube after light is split by the optical gratings, a light signal is converted into an electric signal by the photomultiplier tube, and finally, the electric signal is transmitted to the A/D converter for processing. According to the measuring device for the aflatoxin, the fluorescent light is induced by adopting the laser light source, and the detection sensitivity is greatly enhanced; the measuring device for the aflatoxin is suitable for detecting the trace quantity of the aflatoxin in agricultural products and foods; and the operation is simple and convenient, and the detection cost is low.
Description
Technical field
The utility model relates to a kind of aflatoxin measurement mechanism.
Background technology
Aflatoxin (Aflatoxins) mainly be by Aspergillus flavus (
Aspergillus flavs) and the aspergillus parasiticus bacterium (
Aspergillus parasiticus) the high poison and the strong carcinogenic secondary metabolite that produce, extensively be present in multiple agricultural product and the food.But because the aflatoxin physicochemical property is stable, it is very difficult or even impossible thoroughly it being removed from agricultural product and food fully.Therefore seem very important for examination of aflatoxin.Existing examination of aflatoxin method mainly comprises high performance liquid chromatography, fluorescence opacimeter method, mass spectroscopy etc.The outstanding feature of these class methods is that accuracy of measurement is higher, good reproducibility, but need Large-Scale Precision Instrument and Equipment, the labor organic solvent needs chemical reagent to derive to improve detection sensitivity.Therefore highly sensitive and simple to operate, the low aflatoxin detection technique of detection cost is the focus of agricultural product and food hygiene and quality safety detection research always.
Summary of the invention
The utility model technical matters to be solved is to provide detection sensitivity high, easy and simple to handle aflatoxin measurement mechanism to the deficiency of above-mentioned prior art.
The utility model is that the technical scheme that problem adopted of the above-mentioned proposition of solution is:
It comprises excitation source, sample detection pond, signal measurement treating apparatus and reading device; It is characterized in that: described excitation source is that the LASER Light Source of 375 ± 5 nm laser is formed with conduction optical fiber by the ability emission wavelength; Described signal measurement treating apparatus is to be distributed successively by condenser lens, grating, photomultiplier and A/D converter to form, and described condenser lens is positioned at exciting light and becomes 90
oDirection, the fluorescence signal line focus lens focus that the sample in the sample detection pond is produced arrives photomultiplier again behind grating beam splitting, photomultiplier changes light signal into electric signal, is sent to A/D converter at last and handles.
Press such scheme, said LASER Light Source is connected coupling with conduction optical fiber.
Press such scheme, described LASER Light Source adopts diode pumped solid state laser, and output power is 10～30mW.
Press such scheme, the fiber power of described laser after the coupling of conduction optical fiber is more than 5 mW.General fluorescence analysis excitating light strength optimization range is between 0.5～5 mW, and the optical fiber output power that the utility model adopts is more than 5 mW, and this can improve the fluorescence intensity of aflatoxin, thereby improves detection sensitivity.
Press such scheme, described sample detection pond material is selected quartz glass for use, and it does not have absorption to ultraviolet light.
Pressing such scheme, is the light of 440 ± 10 nm but described grating is set to beam split acquisition wavelength.
The design concept of the utility model is the physicochemical property according to the aflatoxin molecular fluorescence; Adopt LASER Light Source to substitute conventional light source; Required focusing and light-dividing device when having avoided using conventional light source as excitation source; Strengthen the fluorescence intensity of excitating light strength greatly, thereby promoted examination of aflatoxin sensitivity, also reduced the sample size in the sample detection pond simultaneously with raising aflatoxin molecular emission; Simplify the sample pre-treatments operation, reduced the consumption of sample pre-treatments reagent.
The beneficial effect of the utility model is: 1. the aflatoxin measurement mechanism detection sensitivity that provides of the utility model is high, is suitable for the trace detection of aflatoxin in agricultural product and the food.2. the utility model is owing to adopt LASER Light Source to substitute conventional light source; Required focusing and light-dividing device when having avoided using conventional light source as excitation source; Reduced the sample size in the sample detection pond; Reduce the consumption of sample pre-treatments reagent simultaneously, reduced the detection cost, simplified the sample pre-treatments operation.
Description of drawings
Fig. 1 is an aflatoxin measurement mechanism synoptic diagram.
Embodiment
Below in conjunction with accompanying drawing and embodiment the summary of the invention of the utility model is described further.
Embodiment 1
Like Fig. 1; The aflatoxin measurement mechanism comprises LASER Light Source 1; Conduction optical fiber 2 with the excitation source coupling; Sample detection pond 3, by signal measurement treating apparatus and reading device 8 that condenser lens 4, grating 5, photomultiplier 6, A/D converter 7 distribute successively and form, described condenser lens is positioned at exciting light and becomes 90
oDirection, the fluorescence signal line focus lens focus that the sample in the sample detection pond is produced arrives photomultiplier again behind grating beam splitting, photomultiplier changes light signal into electric signal, is sent to A/D converter at last and handles.Said LASER Light Source adopts diode pumped solid state laser, and output power is 20 mW, and emission wavelength is 375 ± 5 nm.The fiber power of exciting light after the coupling of conduction optical fiber is more than 5 mW.The ultraviolet quartz glass is selected in the sample detection pond for use.It is the light of 440 ± 10 nm that but grating is set to beam split acquisition wavelength.
The example that is determined as with AFB1 in the peanut: in pretreated extract was packed the sample detection pond into, LASER Light Source sent the laser that wavelength is 375 ± 5 nm with peanut sample, after the coupling of conduction optical fiber, conducted to the sample detection pond; And pass testing sample, produce fluorescence behind the absorption of sample exciting light in the sample detection pond, the fluorescence signal line focus lens focus of generation; Through grating beam splitting, obtain the light that wavelength is 440 ± 10 nm again, arrive photomultiplier; Photomultiplier changes light signal into electric signal; Simultaneously electric signal is amplified the back input a/d converter, export to reading device at last and handle, can directly read the result through reading device.
Embodiment 2
Like Fig. 1; The aflatoxin measurement mechanism comprises LASER Light Source 1; Conduction optical fiber 2 with the excitation source coupling; Sample detection pond 3, by signal measurement treating apparatus and reading device 8 that condenser lens 4, grating 5, photomultiplier 6, A/D converter 7 distribute successively and form, described condenser lens is positioned at exciting light and becomes 90
oDirection, the fluorescence signal line focus lens focus that the sample in the sample detection pond is produced arrives photomultiplier again behind grating beam splitting, photomultiplier changes light signal into electric signal, is sent to A/D converter at last and handles.Said LASER Light Source adopts diode pumped solid state laser, and output power is 20 mW, and emission wavelength is 375 ± 5 nm.The fiber power of exciting light after the coupling of conduction optical fiber is more than 5 mW.The ultraviolet quartz glass is selected in the sample detection pond for use.It is the light of 440 ± 5 nm that but grating is set to beam split acquisition wavelength.
The example that is determined as with aflatoxin M in the milk and milk products 1: in pretreated extract was packed the sample detection pond into, LASER Light Source sent the laser that wavelength is 375 ± 5 nm with breast or dairy products, after the coupling of conduction optical fiber, conducted to the sample detection pond; And pass testing sample; Produce fluorescence behind the absorption of sample exciting light in the sample detection pond, the fluorescence signal line focus lens focus of generation is again through grating beam splitting; Obtain the light that wavelength is 440 ± 5 nm; Arrive photomultiplier, photomultiplier changes light signal into electric signal, simultaneously electric signal is amplified the back input a/d converter; Export to reading device at last and handle, can directly read the result through reading device.
Claims (6)
1. aflatoxin measurement mechanism; Comprise excitation source, sample detection pond, signal measurement treating apparatus and reading device; It is characterized in that: described excitation source is that the LASER Light Source of 375 ± 5 nm laser is formed with conduction optical fiber by the ability emission wavelength; Described signal measurement treating apparatus is to be distributed successively by condenser lens, grating, photomultiplier and A/D converter to form, and described condenser lens is positioned at exciting light and becomes 90
oDirection, the fluorescence signal line focus lens focus that the sample in the sample detection pond is produced arrives photomultiplier again behind grating beam splitting, photomultiplier changes light signal into electric signal, is sent to A/D converter at last and handles.
2. aflatoxin measurement mechanism according to claim 1 is characterized in that: said LASER Light Source is connected coupling with conduction optical fiber.
3. aflatoxin measurement mechanism according to claim 1 is characterized in that: described LASER Light Source adopts diode pumped solid state laser, and output power is 10～30 mW.
4. aflatoxin measurement mechanism according to claim 1 is characterized in that: the fiber power of described exciting light after the coupling of conduction optical fiber is more than 5 mW.
5. aflatoxin measurement mechanism according to claim 1 is characterized in that: described sample detection pond material is selected quartz glass for use.
6. aflatoxin measurement mechanism according to claim 1 is characterized in that: but described grating is set to beam split acquisition wavelength is the light of 440 ± 10 nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201569914U CN202101939U (en) | 2011-05-17 | 2011-05-17 | Measuring device for aflatoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201569914U CN202101939U (en) | 2011-05-17 | 2011-05-17 | Measuring device for aflatoxin |
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| CN202101939U true CN202101939U (en) | 2012-01-04 |
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| CN2011201569914U Expired - Lifetime CN202101939U (en) | 2011-05-17 | 2011-05-17 | Measuring device for aflatoxin |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103116024A (en) * | 2012-11-30 | 2013-05-22 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528A (en) * | 2012-11-30 | 2013-07-24 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
| CN103308501A (en) * | 2013-05-31 | 2013-09-18 | 浙江师范大学 | Detecting and warning system for monitoring whether milk contains melamine |
| CN105044062A (en) * | 2015-07-31 | 2015-11-11 | 合肥美亚光电技术股份有限公司 | Online aflatoxin detecting device and material sorting equipment adopting same |
| CN105445253A (en) * | 2015-11-12 | 2016-03-30 | 北京农业智能装备技术研究中心 | Equipment for detecting concentration of antibiotics in water |
| CN107144554A (en) * | 2017-06-16 | 2017-09-08 | 合肥泰禾光电科技股份有限公司 | A kind of aflatoxin detection means |
| CN111650123A (en) * | 2020-06-22 | 2020-09-11 | 广东省测试分析研究所（中国广州分析测试中心） | Aflatoxin in-situ derived fluorescence detection device |
-
2011
- 2011-05-17 CN CN2011201569914U patent/CN202101939U/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103116024A (en) * | 2012-11-30 | 2013-05-22 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528A (en) * | 2012-11-30 | 2013-07-24 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
| CN103217528B (en) * | 2012-11-30 | 2014-04-09 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
| CN103116024B (en) * | 2012-11-30 | 2014-04-09 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103308501A (en) * | 2013-05-31 | 2013-09-18 | 浙江师范大学 | Detecting and warning system for monitoring whether milk contains melamine |
| CN105044062A (en) * | 2015-07-31 | 2015-11-11 | 合肥美亚光电技术股份有限公司 | Online aflatoxin detecting device and material sorting equipment adopting same |
| CN105044062B (en) * | 2015-07-31 | 2018-03-23 | 合肥美亚光电技术股份有限公司 | Aflatoxin on-line measuring device and the material separation device using the device |
| CN105445253A (en) * | 2015-11-12 | 2016-03-30 | 北京农业智能装备技术研究中心 | Equipment for detecting concentration of antibiotics in water |
| CN105445253B (en) * | 2015-11-12 | 2018-05-18 | 北京农业智能装备技术研究中心 | A kind of equipment for detecting antibiotic concentration in water |
| CN107144554A (en) * | 2017-06-16 | 2017-09-08 | 合肥泰禾光电科技股份有限公司 | A kind of aflatoxin detection means |
| CN111650123A (en) * | 2020-06-22 | 2020-09-11 | 广东省测试分析研究所（中国广州分析测试中心） | Aflatoxin in-situ derived fluorescence detection device |
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