CN202101939U - Measuring device for aflatoxin - Google Patents
Measuring device for aflatoxin Download PDFInfo
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- CN202101939U CN202101939U CN2011201569914U CN201120156991U CN202101939U CN 202101939 U CN202101939 U CN 202101939U CN 2011201569914 U CN2011201569914 U CN 2011201569914U CN 201120156991 U CN201120156991 U CN 201120156991U CN 202101939 U CN202101939 U CN 202101939U
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- 229930195730 Aflatoxin Natural products 0.000 title claims abstract description 25
- 239000005409 aflatoxin Substances 0.000 title claims abstract description 25
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 36
- 230000005284 excitation Effects 0.000 claims abstract description 21
- 238000012545 processing Methods 0.000 claims abstract description 15
- 238000005259 measurement Methods 0.000 claims abstract description 10
- 239000013307 optical fiber Substances 0.000 claims abstract description 10
- 230000003287 optical effect Effects 0.000 claims abstract description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 7
- 235000013305 food Nutrition 0.000 abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 241001553178 Arachis glabrata Species 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000228230 Aspergillus parasiticus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002115 aflatoxin B1 Substances 0.000 description 1
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 1
- 229930073161 aflatoxin M1 Natural products 0.000 description 1
- 239000002108 aflatoxin M1 Substances 0.000 description 1
- MJBWDEQAUQTVKK-IAGOWNOFSA-N aflatoxin M1 Chemical compound C=1([C@]2(O)C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O MJBWDEQAUQTVKK-IAGOWNOFSA-N 0.000 description 1
- 229930020125 aflatoxin-B1 Natural products 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
本实用新型涉及一种黄曲霉毒素测量装置。黄曲霉毒素测量装置，其特征在于：它包括激发光源、样品检测池、信号测量处理装置和读数装置，其特征在于：所述的激发光源由能发射波长为375±5nm激光的激光光源和传导光纤组成，所述的信号测量处理装置是由聚焦透镜、光栅、光电倍增管和A/D转换器依次分布而成，所述的聚焦透镜位于与激发光成90o的方向，使样品检测池中的样品产生的荧光信号经聚焦透镜聚焦，再经光栅分光后到达光电倍增管，光电倍增管将光信号转变为电信号，最后传送至A/D转换器进行处理。其采用激光光源诱导荧光，大大提高了检测灵敏度，适于农产品及食品中黄曲霉毒素的痕量检测；操作简便，检测成本低。
The utility model relates to an aflatoxin measuring device. The aflatoxin measurement device is characterized in that: it comprises an excitation light source, a sample detection pool, a signal measurement processing device and a reading device, and is characterized in that: the excitation light source is a laser light source and a conductive Composed of optical fibers, the signal measurement and processing device is composed of a focusing lens, a grating, a photomultiplier tube, and an A/D converter. The fluorescent signal generated by the sample in the sample is focused by the focusing lens, and then reaches the photomultiplier tube after being split by the grating. The photomultiplier tube converts the optical signal into an electrical signal, and finally transmits it to the A/D converter for processing. It uses a laser light source to induce fluorescence, which greatly improves the detection sensitivity, and is suitable for the trace detection of aflatoxins in agricultural products and foods; the operation is simple and the detection cost is low.
Description
技术领域 technical field
本实用新型涉及一种黄曲霉毒素测量装置。 The utility model relates to an aflatoxin measuring device.
背景技术 Background technique
黄曲霉毒素（Aflatoxins）主要是由黄曲霉菌（Aspergillus flavs）和寄生曲霉菌（Aspergillus parasiticus）产生的高毒和强致癌的次生代谢产物，广泛存在于多种农产品及食品中。但由于黄曲霉毒素理化性质稳定，要彻底将其从农产品及食品中完全去除是极为困难甚至是不可能的。因此对于黄曲霉毒素的检测显得极为重要。现有黄曲霉毒素的检测方法主要包括高效液相色谱法、荧光光密度计法、质谱法等。这类方法的突出特点是测定准确度较高，重复性好，但需要大型精密仪器设备，耗费大量有机溶剂，需要化学试剂进行衍生以提高检测灵敏度。因此灵敏度高、且操作简单、检测成本低的黄曲霉毒素检测技术一直是农产品及食品卫生和质量安全检测研究的热点。 Aflatoxins are mainly highly toxic and carcinogenic secondary metabolites produced by Aspergillus flavs and Aspergillus parasiticus , and are widely found in a variety of agricultural products and foods. However, due to the stable physical and chemical properties of aflatoxin, it is extremely difficult or even impossible to completely remove it from agricultural products and food. Therefore, the detection of aflatoxin is extremely important. Existing aflatoxin detection methods mainly include high performance liquid chromatography, fluorescence densitometer, mass spectrometry and the like. The prominent features of this type of method are high measurement accuracy and good repeatability, but they require large precision instruments and equipment, consume a large amount of organic solvents, and require chemical reagents to be derivatized to improve detection sensitivity. Therefore, aflatoxin detection technology with high sensitivity, simple operation and low detection cost has always been a hot spot in the research of agricultural products and food hygiene and quality safety detection.
发明内容 Contents of the invention
本实用新型所要解决的技术问题是针对上述现有技术的不足而提供检测灵敏度高、操作简便的黄曲霉毒素测量装置。 The technical problem to be solved by the utility model is to provide an aflatoxin measuring device with high detection sensitivity and easy operation for the deficiencies of the above-mentioned prior art.
本实用新型为解决上述提出的问题所采用的技术方案为： The technical solution adopted by the utility model for solving the problems mentioned above is:
它包括激发光源、样品检测池、信号测量处理装置和读数装置，其特征在于：所述的激发光源由能发射波长为375±5 nm激光的激光光源和传导光纤组成，所述的信号测量处理装置是由聚焦透镜、光栅、光电倍增管和A/D转换器依次分布而成，所述的聚焦透镜位于与激发光成90o的方向，使样品检测池中的样品产生的荧光信号经聚焦透镜聚焦，再经光栅分光后到达光电倍增管，光电倍增管将光信号转变为电信号，最后传送至A/D转换器进行处理。 It includes an excitation light source, a sample detection cell, a signal measurement processing device and a reading device, and is characterized in that: the excitation light source is composed of a laser light source capable of emitting a laser with a wavelength of 375 ± 5 nm and a conductive optical fiber, and the signal measurement processing The device is composed of a focusing lens, a grating, a photomultiplier tube and an A/D converter. The focusing lens is located at a direction of 90 o to the excitation light, so that the fluorescent signal generated by the sample in the sample detection cell is focused The lens is focused, and after being split by the grating, it reaches the photomultiplier tube. The photomultiplier tube converts the optical signal into an electrical signal, and finally transmits it to the A/D converter for processing.
按上述方案，所述激光光源与传导光纤连接耦合。 According to the above solution, the laser light source is connected and coupled with the conductive optical fiber.
按上述方案，所述的激光光源采用半导体泵浦固体激光器，输出功率为10～30mW。 According to the above solution, the laser light source is a semiconductor-pumped solid-state laser with an output power of 10-30 mW.
按上述方案，所述的激光经传导光纤耦合后的出纤功率为5 mW以上。一般荧光分析激发光强度优化范围在0.5～5 mW之间，而本实用新型采用的光纤输出功率为5 mW以上，这可提高黄曲霉毒素的荧光强度，从而提高检测灵敏度。 According to the above scheme, the output power of the laser light coupled through the conducting fiber is above 5 mW. The optimal range of excitation light intensity for general fluorescence analysis is between 0.5 and 5 mW, while the output power of the optical fiber used in the utility model is above 5 mW, which can increase the fluorescence intensity of aflatoxin, thereby improving the detection sensitivity.
按上述方案，所述的样品检测池材质选用石英玻璃，其对紫外光无吸收。 According to the above scheme, the material of the sample detection cell is made of quartz glass, which has no absorption of ultraviolet light.
按上述方案，所述的光栅设置为可分光获得波长为440±10 nm的光。 According to the above scheme, the grating is set to be able to split light to obtain light with a wavelength of 440±10 nm.
本实用新型的设计原理是根据黄曲霉毒素分子荧光的理化特性，采用激光光源替代传统光源，避免了使用传统光源用作激发光源时所需的聚焦和分光装置，大大增强激发光强度以提高黄曲霉毒素分子发射的荧光强度，从而提升了黄曲霉毒素的检测灵敏度，同时也减少了样品检测池中的样品量，简化了样品前处理操作，减少了样品前处理试剂的消耗。 The design principle of the utility model is based on the physical and chemical characteristics of the fluorescence of aflatoxin molecules, using a laser light source instead of a traditional light source, avoiding the need for focusing and splitting devices when using a traditional light source as an excitation light source, and greatly enhancing the intensity of excitation light to improve yellow The fluorescence intensity emitted by the aspergillus toxin molecule improves the detection sensitivity of aflatoxin, and also reduces the sample amount in the sample detection cell, simplifies the sample pretreatment operation, and reduces the consumption of sample pretreatment reagents.
本实用新型的有益效果在于：1. 本实用新型提供的黄曲霉毒素测量装置检测灵敏度高，适于农产品及食品中黄曲霉毒素的痕量检测。2. 本实用新型由于采用激光光源替代传统光源，避免了使用传统光源用作激发光源时所需的聚焦和分光装置，减少了样品检测池中的样品量，同时减少了样品前处理试剂的消耗，降低了检测成本，简化了样品前处理操作。 The beneficial effects of the utility model are: 1. The aflatoxin measuring device provided by the utility model has high detection sensitivity, and is suitable for trace detection of aflatoxin in agricultural products and food. 2. Since the utility model adopts the laser light source instead of the traditional light source, it avoids the focusing and spectroscopic devices required when using the traditional light source as the excitation light source, reduces the sample amount in the sample detection pool, and reduces the consumption of sample pretreatment reagents at the same time , which reduces the detection cost and simplifies the sample pretreatment operation.
附图说明 Description of drawings
图1为黄曲霉毒素测量装置示意图。 Figure 1 is a schematic diagram of the aflatoxin measuring device.
具体实施方式 Detailed ways
下面结合附图和实施例对本实用新型的发明内容作进一步说明。 Below in conjunction with accompanying drawing and embodiment the content of the invention of the present utility model is described further.
实施例1 Example 1
如图1，黄曲霉毒素测量装置包括激光光源1，与激发光源耦合的传导光纤2，样品检测池3，由聚焦透镜4、光栅5、光电倍增管6、A/D转换器7依次分布而成的信号测量处理装置和读数装置8，所述的聚焦透镜位于与激发光成90o的方向，使样品检测池中的样品产生的荧光信号经聚焦透镜聚焦，再经光栅分光后到达光电倍增管，光电倍增管将光信号转变为电信号，最后传送至A/D转换器进行处理。所述激光光源采用半导体泵浦固体激光器，输出功率为20 mW，发射波长为375±5 nm。激发光经传导光纤耦合后的出纤功率为5 mW以上。样品检测池选用紫外石英玻璃。光栅设置为可分光获得波长为440±10 nm的光。 As shown in Figure 1, the aflatoxin measuring device includes a laser light source 1, a conductive optical fiber 2 coupled with an excitation light source, and a sample detection cell 3, which are sequentially distributed by a focusing lens 4, a grating 5, a photomultiplier tube 6, and an A/D converter 7. The signal measurement processing device and the reading device 8 are formed. The focusing lens is located in the direction of 90 o to the excitation light, so that the fluorescent signal generated by the sample in the sample detection cell is focused by the focusing lens, and then separated by the grating to reach the photomultiplier. The photomultiplier tube converts the optical signal into an electrical signal, and finally sends it to the A/D converter for processing. The laser light source is a semiconductor-pumped solid-state laser with an output power of 20 mW and an emission wavelength of 375±5 nm. The output power of the excitation light after coupling through the conducting fiber is above 5 mW. The sample detection cell is made of ultraviolet quartz glass. The grating is set to split light to obtain light with a wavelength of 440±10 nm.
以花生中黄曲霉毒素B1的测定为例：将花生样品经预处理后的提取物装入样品检测池中，激光光源发出波长为375±5 nm的激光，经传导光纤耦合后传导至样品检测池，并穿过待测样品，样品检测池中的样品吸收激发光后产生荧光，产生的荧光信号经聚焦透镜聚焦，再经光栅分光，得到波长为440±10 nm的光，到达光电倍增管，光电倍增管将光信号转变为电信号，同时将电信号放大后输入A/D转换器，最后输出给读数装置处理，通过读数装置即可直接读出结果。 Take the determination of aflatoxin B1 in peanuts as an example: put the pretreated peanut sample extract into the sample detection cell, the laser light source emits laser light with a wavelength of 375±5 nm, which is coupled by a conductive optical fiber and then transmitted to the sample for detection The sample in the sample detection cell absorbs the excitation light and generates fluorescence. The generated fluorescence signal is focused by the focusing lens, and then separated by the grating to obtain light with a wavelength of 440±10 nm, which reaches the photomultiplier tube. , The photomultiplier tube converts the optical signal into an electrical signal, and at the same time, the electrical signal is amplified and input to the A/D converter, and finally output to the reading device for processing, and the result can be directly read out through the reading device.
实施例2 Example 2
如图1，黄曲霉毒素测量装置包括激光光源1，与激发光源耦合的传导光纤2，样品检测池3，由聚焦透镜4、光栅5、光电倍增管6、A/D转换器7依次分布而成的信号测量处理装置和读数装置8，所述的聚焦透镜位于与激发光成90o的方向，使样品检测池中的样品产生的荧光信号经聚焦透镜聚焦，再经光栅分光后到达光电倍增管，光电倍增管将光信号转变为电信号，最后传送至A/D转换器进行处理。所述激光光源采用半导体泵浦固体激光器，输出功率为20 mW，发射波长为375±5 nm。激发光经传导光纤耦合后的出纤功率为5 mW以上。样品检测池选用紫外石英玻璃。光栅设置为可分光获得波长为440±5 nm的光。 As shown in Figure 1, the aflatoxin measuring device includes a laser light source 1, a conductive optical fiber 2 coupled with an excitation light source, and a sample detection cell 3, which are sequentially distributed by a focusing lens 4, a grating 5, a photomultiplier tube 6, and an A/D converter 7. The signal measurement processing device and the reading device 8 are formed. The focusing lens is located in the direction of 90 o to the excitation light, so that the fluorescent signal generated by the sample in the sample detection cell is focused by the focusing lens, and then separated by the grating to reach the photomultiplier. The photomultiplier tube converts the optical signal into an electrical signal, and finally sends it to the A/D converter for processing. The laser light source is a semiconductor-pumped solid-state laser with an output power of 20 mW and an emission wavelength of 375±5 nm. The output power of the excitation light after coupling through the conducting fiber is above 5 mW. The sample detection cell is made of ultraviolet quartz glass. The grating is set to split light to obtain light with a wavelength of 440±5 nm.
以乳及乳制品中黄曲霉毒素M1的测定为例：将乳或乳制品经预处理后的提取物装入样品检测池中，激光光源发出波长为375±5 nm的激光，经传导光纤耦合后传导至样品检测池，并穿过待测样品，样品检测池中的样品吸收激发光后产生荧光，产生的荧光信号经聚焦透镜聚焦，再经光栅分光，得到波长为440±5 nm的光，到达光电倍增管，光电倍增管将光信号转变为电信号，同时将电信号放大后输入A/D转换器，最后输出给读数装置处理，通过读数装置即可直接读出结果。 Take the determination of aflatoxin M1 in milk and dairy products as an example: put the pretreated extract of milk or dairy products into the sample detection cell, and the laser light source emits a laser with a wavelength of 375±5 nm, which is coupled through a conductive optical fiber. After that, it is transmitted to the sample detection cell and passes through the sample to be tested. The sample in the sample detection cell absorbs the excitation light and generates fluorescence. The generated fluorescence signal is focused by the focusing lens and then split by the grating to obtain light with a wavelength of 440±5 nm. , reaches the photomultiplier tube, the photomultiplier tube converts the optical signal into an electrical signal, and at the same time amplifies the electrical signal and inputs it to the A/D converter, and finally outputs it to the reading device for processing, and the result can be directly read out through the reading device.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103116024A (en) * | 2012-11-30 | 2013-05-22 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528A (en) * | 2012-11-30 | 2013-07-24 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
| CN103308501A (en) * | 2013-05-31 | 2013-09-18 | 浙江师范大学 | Detecting and warning system for monitoring whether milk contains melamine |
| CN105044062A (en) * | 2015-07-31 | 2015-11-11 | 合肥美亚光电技术股份有限公司 | Online aflatoxin detecting device and material sorting equipment adopting same |
| CN105445253A (en) * | 2015-11-12 | 2016-03-30 | 北京农业智能装备技术研究中心 | Equipment for detecting concentration of antibiotics in water |
| CN107144554A (en) * | 2017-06-16 | 2017-09-08 | 合肥泰禾光电科技股份有限公司 | A kind of aflatoxin detection means |
| CN111650123A (en) * | 2020-06-22 | 2020-09-11 | 广东省测试分析研究所（中国广州分析测试中心） | Aflatoxin in-situ derived fluorescence detection device |
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2011
- 2011-05-17 CN CN2011201569914U patent/CN202101939U/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103116024A (en) * | 2012-11-30 | 2013-05-22 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528A (en) * | 2012-11-30 | 2013-07-24 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
| CN103116024B (en) * | 2012-11-30 | 2014-04-09 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528B (en) * | 2012-11-30 | 2014-04-09 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
| CN103308501A (en) * | 2013-05-31 | 2013-09-18 | 浙江师范大学 | Detecting and warning system for monitoring whether milk contains melamine |
| CN105044062A (en) * | 2015-07-31 | 2015-11-11 | 合肥美亚光电技术股份有限公司 | Online aflatoxin detecting device and material sorting equipment adopting same |
| CN105044062B (en) * | 2015-07-31 | 2018-03-23 | 合肥美亚光电技术股份有限公司 | Aflatoxin on-line measuring device and the material separation device using the device |
| CN105445253A (en) * | 2015-11-12 | 2016-03-30 | 北京农业智能装备技术研究中心 | Equipment for detecting concentration of antibiotics in water |
| CN105445253B (en) * | 2015-11-12 | 2018-05-18 | 北京农业智能装备技术研究中心 | A kind of equipment for detecting antibiotic concentration in water |
| CN107144554A (en) * | 2017-06-16 | 2017-09-08 | 合肥泰禾光电科技股份有限公司 | A kind of aflatoxin detection means |
| CN111650123A (en) * | 2020-06-22 | 2020-09-11 | 广东省测试分析研究所（中国广州分析测试中心） | Aflatoxin in-situ derived fluorescence detection device |
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