CN109200120B

CN109200120B - Medicine for treating spleen-kidney yang deficiency chronic cough and application thereof

Info

Publication number: CN109200120B
Application number: CN201811412218.2A
Authority: CN
Inventors: 姜廷枢; 杨文君
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-11-25
Filing date: 2018-11-25
Publication date: 2021-03-23
Anticipated expiration: 2038-11-25
Also published as: CN109200120A

Abstract

The invention belongs to the field of medicines, and particularly relates to a medicine for treating spleen-kidney yang deficiency chronic cough and application thereof. The medicine is prepared from platycodon grandiflorum, aster, schizonepeta, dried orange peel and red cudrania cochinchinensis serving as raw materials. The weight ratio of the raw materials is 9-20 parts of platycodon grandiflorum, 9-20 parts of aster tataricus, 9-20 parts of schizonepeta, 5-10 parts of dried orange peel and 5-7 parts of red cudrania cochinchinensis. In the preparation process, the raw materials are extracted into volatile oil components by a steam distillation method. The medicine for treating spleen-kidney yang deficiency chronic cough has a certain cough relieving effect on a spleen-kidney yang deficiency chronic cough animal model.

Description

Medicine for treating spleen-kidney yang deficiency chronic cough and application thereof

Technical Field

The invention belongs to the field of medicines, and particularly relates to a medicine for treating spleen-kidney yang deficiency chronic cough and application thereof.

Background

Cough is a common clinical symptom, and cold or other stimulating factors such as capsaicin and smoking can cause cough reflex of respiratory tract. For the cough accompanied with symptoms such as turbid phlegm and the like, the cough belongs to a protective reaction mechanism, and turbid phlegm and deposited particles in the respiratory tract can be discharged through the cough, so that the respiratory tract is protected. However, the hyperreactivity of cough can also damage respiratory tract, so that antitussive medicines are often used in clinic to avoid the harm caused by excessive cough. Clinical cough is classified into wet cough and dry cough according to the presence or absence of sputum, and infectious cough and noninfectious cough according to the presence or absence of infection. The disease course is divided into acute cough, chronic cough and the like.

Modern medicine believes that cough is a physiological reflex triggered by the activation of cough receptors in the body. The interaction enhancement of mechanical and chemical receptors is the pathophysiological basis for chronic cough caused by certain diseases. Based on neurological cognition on cough, most of cough relieving medicines in the current western medicines are central and peripheral nerve inhibitors. Most of the central antitussive medicines such as benproperine phosphate, pentoxyverine citrate and dextromethorphan hydrobromide for treating dry cough are central antitussive medicines. The medicine directly acts on related receptors, has quick response, but the inhibition effect of the central or peripheral nerve of the medicine can also cause a plurality of adverse reactions, such as lethargy and the like.

The traditional Chinese medicine holds that the main pathogenesis of cough is the failure of dispersing and descending of the lung and the adverse ascending of lung qi. Cough is caused by the failure of dispersing and descending of the lung due to both exogenous factors and internal injury, for example, cough caused by exogenous pathogenic factors attacking the lung failing to disperse and descend, or cough caused by internal injury of internal organs. The "Nei Jing" has cloud: the five zang-organs and six fu-organs all cause cough. For example, the lung and large intestine are exterior and interior, and the large intestine is excessive heat and obstructed by qi of the fu-organs, which may cause cough and dyspnea. Spleen deficiency failing to transport and phlegm-dampness leading to internal generation can lead to cough due to lung qi obstruction. Kidney qi deficiency can affect the dispersing and descending of lung qi, which can lead to cough and dyspnea. Therefore, in TCM, cough is manifested in the lung, but its cause may be in other organs, such as large intestine, liver, spleen, kidney, etc. Among them, the occurrence of cough can be caused by the insufficiency of both the spleen and the kidney.

The traditional Chinese medicine for treating cough is mostly a medicine for eliminating phlegm, relieving cough and asthma. According to the different actions of herbs, the actions can be classified into drying dampness and resolving phlegm, warming and resolving cold phlegm, clearing and resolving heat phlegm, moistening dryness and resolving phlegm, etc. Cough and asthma relieving herbs can also be classified into lung ventilating, lung descending, lung purging, lung clearing, lung moistening, lung astringing, etc. The commonly used cough-relieving traditional Chinese medicines include:

herbs for warming and resolving cold-phlegm: is mainly used for treating cough and asthma caused by cold phlegm invading the lung, and phlegm-dampness blocking the channels and collaterals to cause the obstruction of phlegm-core flow and the like. The commonly used herbs include ban Xia, Tian nan xing, Bai Fu Zi, Bai Jie Zi, Jing Jie and Jie Geng, etc.

Heat-phlegm-clearing herbs: is mainly used for treating excessive phlegm cough and asthma, dry cough with little phlegm and unsmooth expectoration caused by heat-phlegm obstructing in the lung. The commonly used herbs include Peucedanum praeruptorum, Trichosanthes kirilowii Maxim, Fritillaria thunbergii, caulis Bambusae in Taenia, succus Bambusae, Sargassum, thallus laminariae, lapis Micae aureus, semen Scaphii Lychnophori, etc.

Antitussive and antiasthmatic herbs: it is mainly used for cough and dyspnea due to lung qi loss, dispersion and descending, phlegm accumulation and adverse rising of qi. The commonly used medicines include semen Armeniacae amarum, Perillae herba, cortex Mori, herba Ardisiae Japonicae, herba Gnaphalii affinis, flos Daturae Metelis, radix Platycodi, radix Asteris, flos Farfarae, semen Ginkgo, etc

Through long-term clinical demonstration, some traditional Chinese medicine compound has proved to have exact curative effect on cough. For example Bulbus Fritillariae Cirrhosae cough syrup, is prepared from Bulbus Fritillariae Cirrhosae, semen Armeniacae amarum solution, flos Farfarae, folium Perillae, radix Asteris, radix Peucedani, radix Platycodi, pericarpium Citri Tangerinae, Glycyrrhrizae radix, sucrose, and sodium benzoate, and can be widely used in treating cough. The cough relieving powder is prepared from platycodon grandiflorum, schizonepeta, aster tataricus, platycodon grandiflorum, schizonepeta, liquorice and dried orange peel, is mild and mild, is warm but not dry, can generate a plurality of plus or minus prescriptions according to the addition or subtraction of symptoms, and is quite wide in clinical application.

Disclosure of Invention

In view of the prior art, one of the purposes of the present invention is to provide a medicine for treating chronic cough due to spleen-kidney yang deficiency. In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a medicine for treating chronic cough due to spleen-kidney yang deficiency is prepared from Chinese medicinal materials including radix Platycodi, radix Asteris, herba Schizonepetae, and pericarpium Citri Tangerinae.

Preferably, the medicine for treating chronic cough due to spleen-kidney yang deficiency is prepared from platycodon grandiflorum, aster tataricus, schizonepeta, dried orange peel and radix cudraniae. Wherein the Platycodon grandiflorum is dried root of Platycodon grandiflorum (Jacq.) A.DC. of Campanulaceae. Bitter, pungent and mild in nature. Meridian tropism enters lung meridian. The functions are mainly used for dispersing lung qi, relieving sore throat, eliminating phlegm and expelling pus. Can be used for treating cough with excessive phlegm, chest distress, pharyngalgia, hoarseness, lung carbuncle, suppuration, and pyocutaneous disease with pus formation. Radix Asteris is the dried root and rhizome of Asteris tataricus L. Pungent, bitter and warm in nature. Meridian tropism enters lung meridian. The functions are mainly moistening lung and descending qi, and dissolving phlegm and relieving cough. Can be used for treating excessive phlegm, cough, chronic cough, and cough and hemoptysis. Herba Schizonepetae is dried aerial part of herba Schizonepetae of Labiatae, Schizonepeta tenuifolia Briq. Pungent in nature and slightly warm in flavor. Meridian tropism enters lung and liver meridians. The functions are mainly used for relieving exterior syndrome, dispelling wind and promoting eruption. Can be used for treating common cold, headache, measles, rubella, and initial stage of pyocutaneous disease. Stir-baked into charcoal is indicated for hematochezia, metrorrhagia and metrostaxis and postpartum anemic fainting. The pericarpium Citri Tangerinae is dried mature pericarp of Rutaceae plant Citrus reticulata Blanco and its cultivar. Bitter and pungent in property and warm in nature. Meridian tropism enters lung and spleen meridians. The functions are mainly used for regulating qi, strengthening spleen, eliminating dampness and resolving phlegm. Can be used for treating fullness in chest and abdomen, anorexia, vomiting, diarrhea, cough, and excessive phlegm. The radix Seu caulis Erythrinae is root and leaf of Nanling Oak of Hydnocarpus. Latin plant animal mineral name: xylosma controlverse Clos; pungent taste; sweet; is cold. Excels in dissipating blood stasis and relieving swelling; cool blood and stop bleeding. Mainly treats traumatic injuries; fracture of the bone; dislocation; traumatic hemorrhage; hematemesis; scald and burn.

Preferably, the Chinese medicinal raw materials including platycodon grandiflorum, aster tataricus, schizonepeta, dried orange peel and radix cudraniae are extracted by a steam distillation method.

Further preferably, the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicinal materials in parts by weight: 9-20 parts of platycodon grandiflorum, 9-20 parts of aster tataricus, 9-20 parts of schizonepeta, 5-10 parts of dried orange peel and 5-7 parts of red cudrania cochinchinensis.

As one preferable scheme, the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicine raw materials in parts by weight: 9 parts of platycodon grandiflorum, 9 parts of aster tataricus, 9 parts of schizonepeta, 5 parts of dried orange peel and 5 parts of radix cynanchi bungei.

As another preferred scheme, the medicament for treating spleen-kidney yang deficiency chronic cough comprises the following Chinese medicinal raw materials in parts by weight: 13 parts of platycodon grandiflorum, 13 parts of radix asteris, 13 parts of schizonepeta, 8 parts of dried orange peel and 6 parts of radix rhodiolae.

As another preferable scheme, the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicine raw materials in parts by weight: 19 parts of platycodon grandiflorum, 19 parts of radix asteris, 19 parts of schizonepeta, 10 parts of dried orange peel and 7 parts of radix rhodiolae.

As another preferable scheme, the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicine raw materials in parts by weight: 19 parts of platycodon grandiflorum, 19 parts of radix asteris, 19 parts of schizonepeta, 7 parts of dried orange peel and 6 parts of radix cynanchi bungei.

For the dosage form of the medicine, the dosage form of the medicine for treating the spleen-kidney yang deficiency chronic cough is oral liquid. Of course, pulmonary inhalation formulations are also possible. Thus being beneficial to the direct action of the medicine on the lung and quickly controlling the cough symptom.

When the dosage form is oral liquid, the steam distillation extract can be directly used as oral liquid preparation, or necessary pharmaceutical adjuvants such as antiseptic, etc. can be added to improve the stability of the medicine during storage. Inclusion with cyclodextrin is also possible. For preparing the oral liquid, the preparation method of the medicament for treating the chronic cough due to spleen-kidney yang deficiency at least comprises the following steps:

(1) respectively crushing platycodon grandiflorum, aster tataricus, schizonepeta, dried orange peel and radix cudraniae according to the prescription amount and sieving the crushed materials with a 30-mesh sieve to obtain crude medicinal powder;

(2) mixing the crude medicinal powder, adding water with the weight being 8-10 times of the total weight of the crude medicinal powder, soaking for 2-6 hours, and then adopting a volatile oil extractor to keep micro-boiling to extract volatile oil with the relative density being less than 1.0, wherein the extraction time is 7-10 hours.

Preferably, the addition amount of the water is 8 times of the total weight of the crude powder of the medicinal materials, the soaking time is 4 hours, and the extraction time is 8 hours. In a case where no particular description is given, the steam distillation extraction process may be carried out by heating to boiling and then heating at 70 ℃ to keep slight boiling.

On the other hand, the invention also provides application of the medicine in preparing a medicine for treating spleen-kidney yang deficiency chronic cough.

The medicine has cough relieving effect on spleen-kidney yang deficiency chronic cough animal model after being extracted and orally administered.

Detailed Description

The invention is further illustrated by the following examples.

EXAMPLE 1 cough relieving Compound volatile oil and extraction

The formula is as follows: 900g of platycodon grandiflorum, 900g of aster tataricus, 900g of schizonepeta, 500g of dried orange peel and 500g of radix cudraniae.

Extraction: (1) respectively crushing platycodon grandiflorum, aster tataricus, schizonepeta, dried orange peel and radix cudraniae according to the prescription amount and sieving the crushed materials with a 30-mesh sieve to obtain crude medicinal powder;

(2) mixing the crude powder, adding water 8 times the total weight of the crude powder, soaking for 4 hr, and extracting volatile oil with relative density less than 1.0 by using volatile oil extractor for 5 hr.

EXAMPLE 2 cough relieving Compound volatile oil and extraction

The formula is as follows: 1300g of platycodon grandiflorum, 1300g of aster tataricus, 1300g of schizonepeta, 800g of dried orange peel and 600g of red cudrania cochinchinensis.

EXAMPLE 3 cough relieving Compound volatile oil and extraction

The formula is as follows: 1900g of platycodon grandiflorum, 1900g of aster tataricus, 1900g of schizonepeta, 1000g of dried orange peel and 700g of radix cudraniae.

EXAMPLE 4 cough relieving Compound volatile oil and extraction

The formula is as follows: 1900g of platycodon grandiflorum, 1900g of aster tataricus, 1900g of schizonepeta, 700g of dried orange peel and 600g of radix cudraniae

Example 5 antitussive effect of antitussive compound drug on chronic cough rats with spleen-kidney yang deficiency

Animal molding: SD rats with male weight of 180-220 g are established with a spleen-kidney yang deficiency chronic cough model in a disease and syndrome combined mode according to a method reported in the literature (Ximenzhou, He-armeng, Qu ya Ting, Zhang Xi, Zhang Zhao Meng, research on a spleen-kidney yang deficiency chronic cough rat model, the university of Hunan traditional Chinese medicine, 2010,39(9): 30-33.) through stimulation of cigarettes to induce cough symptoms, and the spleen-kidney yang deficiency model is established through stimulation of medicines and cold and heat, so that the spleen-kidney yang deficiency chronic cough model of the rats is established.

Wherein the cigarette stimulation is performed twice daily with 6 hour intervals. Rats were placed in standard rat cages of about 380 mm x325 mm x180mm, 6 rats per cage, 10 cigarettes of haddock were lit each time (filter removed), placed in small beakers of equivalent size after lighting, and then covered with a glass dish above the beakers to allow cigarette smoke to escape from the slot above the beakers. The beaker was then placed in a rat cage and secured with tape to prevent tipping over. After the cigarette is burnt, the exhaust system is started to purify the indoor air.

In the interval period of cigarette exposure twice a day, spleen-kidney yang deficiency molding stimulation is carried out, and the senna leaf water decoction is firstly added into each rat by intragastric administration and 0.4ml of each rat is added (the senna leaf water decoction is obtained by adding 10 times of water into senna leaves, decocting for 4h and filtering). After half an hour of gastric lavage, the whole body of each rat is sprayed with ice water at 4 ℃ by a watering can to moisten the skin and hair, and then dried by a fan (the rotating speed is about 1200 rpm).

After four weeks of the above stimulation, the molded rats were randomly divided into six groups of 6 rats each for intragastric administration.

The first group is used as a model group, and the normal saline is administered by intragastric administration, wherein each intragastric administration is 1 ml;

the second group is used as radix Seu caulis Parthenocissi Tricuspidatae control group, and the volatile oil extract is administered by intragastric administration, and the dose per intragastric administration is 0.2ml of volatile oil and 0.8ml of normal saline;

the third group is used as compound control group of radix Platycodi, radix Asteris, herba Schizonepetae, and pericarpium Citri Tangerinae, and the volatile oil extract of radix Platycodi, radix Asteris, herba Schizonepetae, and pericarpium Citri Tangerinae is administered by intragastric administration, and the volatile oil is administered by intragastric administration 0.2ml per each time and is added with physiological saline 0.8 ml;

the volatile oil prepared in example 1, example 2 and example 3 is respectively administrated to the fourth to sixth groups by intragastric administration, and 0.2ml of the volatile oil and 0.8ml of physiological saline are respectively administrated to each intragastric administration.

The preparation method of the red cudrania cochinchinensis control group sample comprises the following steps: pulverizing radix Cudraniae, sieving with 30 mesh sieve, adding 8 times of water, soaking for 4 hr, and extracting volatile oil with relative density less than 1.0 by volatile oil extractor for 5 hr.

The preparation method of the compound control group sample of platycodon grandiflorum, aster tataricus, schizonepeta and dried orange peel comprises the following steps: respectively crushing 1300g of platycodon grandiflorum, 1300g of aster tataricus, 1300g of schizonepeta and 800g of dried orange peel, sieving with a 30-mesh sieve, mixing the medicinal powders, adding 8 times of water, soaking for 4 hours, and then adopting a volatile oil extractor to keep micro-boiling to extract volatile oil with the relative density of less than 1.0, wherein the extraction time is 5 hours.

Each group of rats was administered 1 time daily by gavage for two weeks.

And (5) adopting capsaicin solution to induce cough 8 hours after the last administration. The concentration of the capsaicin solution is 0.15g per 100 ml. A1.5L plastic bottle of the drinking purified water of the Wahaha is taken, and the bottom is removed. The head of the rat is put into a baby haha plastic bottle with 1 bottle per bottle. Then the bottle is vertically placed on a laboratory bench, the hind limb of the rat touches the bottom, the forelimb touches the side wall to be in a state similar to a standing state due to escape resistance, but the bottle mouth in the head of the rat is still distant due to the higher plastic bottle. Meanwhile, due to the limitation of the thickness of the plastic bottle, the body rotation scope of the rat is not enough, but the chest and abdomen have enough motion scope, so that the cough motion can be observed conveniently. At this time, the capsaicin solution was sprayed from the bottle mouth three times per bottle.

The plastic bottle was quickly tightened after the capsaicin solution was sprayed in, and the number of coughs per rat was examined within 5 minutes. Thereby comparing the cough relieving effect of the medicines in each group. The number of coughs is recorded for each cough sound or cough motion (rapid twisting of the head, sharp contraction of the abdomen or chest contraction and mouth opening). The statistical results of the cough frequency of each group of rats are shown in the following table:

comparisons of the number of coughs between groups were performed using the t-test. The results showed that the number of coughs was significantly lower in the administered groups than in the model group (p <0.05 or p < 0.01) except for the rubus delavayi control group. Wherein the number of coughs in the examples 1 to 3 groups was significantly lower than that in the compound control group (p <0.05 or p < 0.01). As can be seen from the statistical results, the volatile oil prepared in the example has better cough relieving effect on model rats

Radix Platycodi, radix Asteris, herba Schizonepetae, and pericarpium Citri Tangerinae.

Claims

1. The medicine for treating the spleen-kidney yang deficiency chronic cough is characterized in that the medicine for treating the spleen-kidney yang deficiency chronic cough is prepared by taking platycodon grandiflorum, aster tataricus, schizonepeta, dried orange peel and red cudrania cochinchinensis as traditional Chinese medicine raw materials, and extracting volatile oil with the relative density of less than 1.0 from the traditional Chinese medicine raw materials by adopting a steam distillation method; the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicine raw materials in parts by weight: 9-20 parts of platycodon grandiflorum, 9-20 parts of aster tataricus, 9-20 parts of schizonepeta, 5-10 parts of dried orange peel and 5-7 parts of red cudrania cochinchinensis.

2. The medicine for treating spleen-kidney yang deficiency chronic cough according to claim 1, wherein the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicinal materials in parts by weight: 9 parts of platycodon grandiflorum, 9 parts of aster tataricus, 9 parts of schizonepeta, 5 parts of dried orange peel and 5 parts of radix cynanchi bungei.

3. The medicine for treating spleen-kidney yang deficiency chronic cough according to claim 1, wherein the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicinal materials in parts by weight: 13 parts of platycodon grandiflorum, 13 parts of radix asteris, 13 parts of schizonepeta, 8 parts of dried orange peel and 6 parts of radix rhodiolae.

4. The medicine for treating spleen-kidney yang deficiency chronic cough according to claim 1, wherein the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following traditional Chinese medicinal materials in parts by weight: 19 parts of platycodon grandiflorum, 19 parts of radix asteris, 19 parts of schizonepeta, 10 parts of dried orange peel and 7 parts of radix rhodiolae.

5. The medicine for treating spleen-kidney yang deficiency chronic cough according to claim 1, wherein the preparation formulation of the medicine for treating spleen-kidney yang deficiency chronic cough is oral liquid.

6. The medicine for treating spleen-kidney yang deficiency chronic cough according to claim 5, wherein the preparation method of the medicine for treating spleen-kidney yang deficiency chronic cough comprises the following steps:

7. The medicine for treating spleen-kidney yang deficiency chronic cough as claimed in claim 6, wherein the amount of water added is 8 times of the total weight of the crude powder of medicinal materials, the soaking time is 4 hours, and the extraction time is 8 hours.

8. The use of the medicament for treating chronic cough due to spleen-kidney yang deficiency of claim 1 in the preparation of a medicament for treating chronic cough due to spleen-kidney yang deficiency.