CN109182450A - A kind of selection of true pleurotus cornucopiae strain - Google Patents
A kind of selection of true pleurotus cornucopiae strain Download PDFInfo
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- CN109182450A CN109182450A CN201811074239.8A CN201811074239A CN109182450A CN 109182450 A CN109182450 A CN 109182450A CN 201811074239 A CN201811074239 A CN 201811074239A CN 109182450 A CN109182450 A CN 109182450A
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Abstract
The invention discloses a kind of selections of true pleurotus cornucopiae strain, and steps are as follows for specific selection: step 1: by sophora bud slag, mixture of Chinese herbal medicines, ash powder, potassium hydrogen phosphate, Pig cholate, glucose, lactose, agar is added in culture medium；Step 2: clean water will be added in the culture medium of step 1, then shakes up, pours into culture dish；Step 3: black line is drawn on each culture dish bottom cover by 2 equal part of area, and is being inoculated with true pleurotus cornucopiae parent species and Antagonistic Fungi respectively in the opposite two o'clock of plate center 3cm；Step 4: inoculated culture dish temperature control is protected from light closed culture for 22 DEG C；Step 5: after culture, calculating true pleurotus cornucopiae strain in each culture dish, to the bacteriostasis rate of Antagonistic Fungi, selecting the true pleurotus cornucopiae strain in the higher culture dish of bacteriostasis rate is the target strain of institute's breeding.The selection of the true pleurotus cornucopiae strain of the invention is simple and convenient, easy to spread；And the energy is not consumed substantially, does not have any pollution to environment, meets the industrial policy of national energy-saving environmental protection.
Description
Technical field
The invention belongs to strain improvement production technical fields, and in particular to a kind of selection of true pleurotus cornucopiae strain.
Background technique
The true pleurotus cornucopiae speed of growth is slower, inhibits the ability of miscellaneous bacteria poor, and the shadow vulnerable to planting environment and cultivation condition
It rings, has compared with other factory edible fungis produce kind and have a certain disadvantage.Strain improvement is that true pleurotus cornucopiae is cultivated in step most
One of key technology makes true pleurotus cornucopiae cultivation also only be confined to small range due to being limited by technology and equipment condition, this is unfavorable for
The all-round popularization of true pleurotus cornucopiae industry is universal.The breeding of existing true pleurotus cornucopiae strain mainly passes through physics and chemical method mutagenesis is turned out
The energetic true pleurotus cornucopiae strain of anti-Trichoderma viride, the method complex process, input cost are high, are not easy to be widely applied.
Summary of the invention
The purpose of the present invention is to provide a kind of selections of true pleurotus cornucopiae strain, to solve to propose in above-mentioned background technique
The breeding of existing true pleurotus cornucopiae strain mainly pass through physics and the energetic true Ji of anti-Trichoderma viride is turned out in chemical method mutagenesis
Mushroom strains, the method complex process, input cost are high, are not easy to the problem of being widely applied.
To achieve the above object, the invention provides the following technical scheme: a kind of selection of true pleurotus cornucopiae strain, specific to select
It is as follows to educate method and step:
Step 1: by sophora bud slag, mixture of Chinese herbal medicines, ash powder, potassium hydrogen phosphate, Pig cholate, glucose, lactose, agar
It is added in culture medium；
Step 2: clean water will be added in the culture medium of step 1, then shakes up, pours into culture dish；
Step 3: black line is drawn on each culture dish bottom cover by 2 equal part of area, and opposite apart from plate center 3cm
True pleurotus cornucopiae parent species and Antagonistic Fungi are inoculated in two o'clock respectively；
Step 4: inoculated culture dish temperature control is protected from light closed culture for 22 DEG C；
Step 5: after culture, calculating true pleurotus cornucopiae strain in each culture dish to the bacteriostasis rate of Antagonistic Fungi, select bacteriostasis rate compared with
True pleurotus cornucopiae strain in high culture dish is the target strain of institute's breeding；
Step 6: multiple groups culture dish is expanded numerous rear culture 14 days, is transferred in triangular flask；It cultivates 37 days and transfers in triangular flask
Into original seed culture bottle；Original seed is transferred in culture bottle after growing 45 days；85 days mycelium stimulation fruitings are grown in cultivation；
Step 7: comparing and analyzing the growing state of pleurotus cornucopiae strain true in step 5 and step 6 multiple groups culture dish,
Obtain final conclusion.
Further, pH value is adjusted to 7 in the step 1, and temperature is adjusted to 23-25 DEG C.
Further, ultrasonic sterilization processing is carried out before raw material is added in culture medium in the step 1.
Further, the culture solution in culture medium is poured into six groups of identical culture dishes in the step 2.
Further, in the step 5 bacteriostasis rate be Antagonistic Fungi colony diameter subtract true pleurotus cornucopiae colony diameter again divided by
The resulting numerical value of Antagonistic Fungi diameter.
Further, the mixture of Chinese herbal medicines includes tung oil root, chervil, gynostemma pentaphylla, tabasheer, nine inner bright and mountain
The root of Dahurain angelica.
Compared with prior art, the beneficial effects of the present invention are:
1, the sophora bud slag is mature sophora bud fresh goods, and sophora bud slag amino acid content is only second to soya-bean cake, is better than wheat, nitrogenous
Amount is in 6% or more, there are also a variety of nutriments such as phosphorus, potassium, best culturing edible fungus fertilizer.
2, mixture of Chinese herbal medicines includes tung oil root, chervil, gynostemma pentaphylla, tabasheer, nine inner bright and the mountain root of Dahurain angelica, inside
Containing the microelement that true pleurotus cornucopiae strain is cultivated, the breeding of true pleurotus cornucopiae strain is provided.
3, the present invention can turn out the energetic true pleurotus cornucopiae of anti-Trichoderma viride without using physics and chemical method mutagenesis
Strain, it is simple and convenient, easy to spread；And the energy is not consumed substantially, does not have any pollution to environment, meets national energy-saving environmental protection
Industrial policy.
4, comparing and analyzing to the growing state of pleurotus cornucopiae strain true in step 5 and step 6 multiple groups culture dish makes breeding
True pleurotus cornucopiae strain out is more in line with standard quality.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
A kind of selection of true pleurotus cornucopiae strain, steps are as follows for specific selection:
Step 1: by sophora bud slag, mixture of Chinese herbal medicines, ash powder, potassium hydrogen phosphate, Pig cholate, glucose, lactose, agar
It is added in culture medium；
Step 2: clean water will be added in the culture medium of step 1, then shakes up, pours into culture dish；
Step 3: black line is drawn on each culture dish bottom cover by 2 equal part of area, and opposite apart from plate center 3cm
True pleurotus cornucopiae parent species and Antagonistic Fungi are inoculated in two o'clock respectively；
Step 4: inoculated culture dish temperature control is protected from light closed culture for 22 DEG C；
Step 5: after culture, calculating true pleurotus cornucopiae strain in each culture dish to the bacteriostasis rate of Antagonistic Fungi, select bacteriostasis rate compared with
True pleurotus cornucopiae strain in high culture dish is the target strain of institute's breeding；
Step 6: multiple groups culture dish is expanded numerous rear culture 14 days, is transferred in triangular flask；It cultivates 37 days and transfers in triangular flask
Into original seed culture bottle；Original seed is transferred in culture bottle after growing 45 days；85 days mycelium stimulation fruitings are grown in cultivation；
Step 7: comparing and analyzing the growing state of pleurotus cornucopiae strain true in step 5 and step 6 multiple groups culture dish,
Obtain final conclusion.
Wherein, pH value is adjusted to 7 in the step 1, and temperature is adjusted to 23-25 DEG C.
Wherein, ultrasonic sterilization processing is carried out before raw material is added in culture medium in the step 1.
Wherein, the culture solution in culture medium is poured into six groups of identical culture dishes in the step 2.
Wherein, bacteriostasis rate is that the colony diameter of Antagonistic Fungi subtracts true pleurotus cornucopiae colony diameter again divided by antagonism in the step 5
The resulting numerical value of bacterium diameter.
Wherein, the mixture of Chinese herbal medicines includes tung oil root, chervil, gynostemma pentaphylla, tabasheer, nine inner bright and the mountain root of Dahurain angelica.
Embodiment 2
A kind of selection of true pleurotus cornucopiae strain, steps are as follows for specific selection:
Step 1: by sophora bud slag, mixture of Chinese herbal medicines, ash powder, calcium superphosphate, gypsum, glucose, lactose, agar adds
Enter into culture medium；
Step 2: clean water will be added in the culture medium of step 1, then shakes up, pours into culture dish；
Step 3: black line is drawn on each culture dish bottom cover by 2 equal part of area, and opposite apart from plate center 3cm
True pleurotus cornucopiae parent species and Antagonistic Fungi are inoculated in two o'clock respectively；
Step 4: inoculated culture dish temperature control is protected from light closed culture for 22 DEG C；
Step 5: after culture, calculating true pleurotus cornucopiae strain in each culture dish to the bacteriostasis rate of Antagonistic Fungi, select bacteriostasis rate compared with
True pleurotus cornucopiae strain in high culture dish is the target strain of institute's breeding；
Step 6: multiple groups culture dish is expanded numerous rear culture 14 days, is transferred in triangular flask；It cultivates 37 days and transfers in triangular flask
Into original seed culture bottle；Original seed is transferred in culture bottle after growing 45 days；85 days mycelium stimulation fruitings are grown in cultivation；
Step 7: comparing and analyzing the growing state of pleurotus cornucopiae strain true in step 5 and step 6 multiple groups culture dish,
Obtain final conclusion.
Wherein, pH value is adjusted to 7 in the step 1, and temperature is adjusted to 23-25 DEG C.
Wherein, ultrasonic sterilization processing is carried out before raw material is added in culture medium in the step 1.
Wherein, the culture solution in culture medium is poured into six groups of identical culture dishes in the step 2.
Wherein, bacteriostasis rate is that the colony diameter of Antagonistic Fungi subtracts true pleurotus cornucopiae colony diameter again divided by antagonism in the step 5
The resulting numerical value of bacterium diameter.
Wherein, the mixture of Chinese herbal medicines includes tung oil root, chervil, gynostemma pentaphylla, tabasheer, nine inner bright and the mountain root of Dahurain angelica.
When the invention works: the sophora bud slag is mature sophora bud fresh goods, and sophora bud slag amino acid content is only second to soya-bean cake, excellent
In wheat, nitrogen content is 6% or more, there are also a variety of nutriments such as phosphorus, potassium, best culturing edible fungus fertilizer, and Chinese herbal medicine
Mixture includes tung oil root, chervil, gynostemma pentaphylla, tabasheer, nine inner bright and the mountain root of Dahurain angelica, and inside is trained containing true pleurotus cornucopiae strain
The microelement educated, provides the breeding of true pleurotus cornucopiae strain, and the present invention can be turned out without using physics and chemical method mutagenesis
The energetic true pleurotus cornucopiae strain of anti-Trichoderma viride, it is simple and convenient, easy to spread；And the energy is not consumed substantially, is not had to environment
Any pollution meets the industrial policy of national energy-saving environmental protection.To pleurotus cornucopiae strain true in step 5 and step 6 multiple groups culture dish
Growing state compares and analyzes the true pleurotus cornucopiae strain for making to select and is more in line with standard quality.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of selection of true pleurotus cornucopiae strain, which is characterized in that steps are as follows for specific selection:
Step 1: by sophora bud slag, mixture of Chinese herbal medicines, ash powder, potassium hydrogen phosphate, Pig cholate, glucose, lactose, agar addition
Into culture medium；
Step 2: clean water will be added in the culture medium of step 1, then shakes up, pours into culture dish；
Step 3: black line is drawn on each culture dish bottom cover by 2 equal part of area, and in the two o'clock opposite apart from plate center 3cm
It is upper to be inoculated with true pleurotus cornucopiae parent species and Antagonistic Fungi respectively；
Step 4: inoculated culture dish temperature control is protected from light closed culture for 22 DEG C；
Step 5: it after culture, calculates true pleurotus cornucopiae strain in each culture dish and selects bacteriostasis rate compared with Gao Pei the bacteriostasis rate of Antagonistic Fungi
Support the target strain that the true pleurotus cornucopiae strain in ware is institute's breeding；
Step 6: multiple groups culture dish is expanded numerous rear culture 14 days, is transferred in triangular flask；It is cultivated 37 days in triangular flask and is transferred to original
In kind culture bottle；Original seed is transferred in culture bottle after growing 45 days；85 days mycelium stimulation fruitings are grown in cultivation.
Step 7: the growing state of pleurotus cornucopiae strain true in step 5 and step 6 multiple groups culture dish is compared and analyzed, is obtained
Final conclusion.
2. a kind of selection of true pleurotus cornucopiae strain according to claim 1, it is characterised in that: by PH in the step 1
Value is adjusted to 7, and temperature is adjusted to 23-25 DEG C.
3. a kind of selection of true pleurotus cornucopiae strain according to claim 1, it is characterised in that: trained in the step 1
It supports before raw material is added in base and carries out ultrasonic sterilization processing.
4. a kind of selection of true pleurotus cornucopiae strain according to claim 1, it is characterised in that: will training in the step 2
The culture solution supported in base is poured into six groups of identical culture dishes.
5. a kind of selection of true pleurotus cornucopiae strain according to claim 1, it is characterised in that: in the step 4 day by day
Record the colony diameter of each culture dish.
6. a kind of selection of true pleurotus cornucopiae strain according to claim 1, it is characterised in that: antibacterial in the step 5
Rate is that the colony diameter of Antagonistic Fungi subtracts true pleurotus cornucopiae colony diameter again divided by the resulting numerical value of Antagonistic Fungi diameter.
7. a kind of selection of true pleurotus cornucopiae strain according to claim 1, it is characterised in that: the mixture of Chinese herbal medicines
Including tung oil root, chervil, gynostemma pentaphylla, tabasheer, nine inner bright and the mountain root of Dahurain angelica.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811074239.8A CN109182450A (en) | 2018-09-14 | 2018-09-14 | A kind of selection of true pleurotus cornucopiae strain |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811074239.8A CN109182450A (en) | 2018-09-14 | 2018-09-14 | A kind of selection of true pleurotus cornucopiae strain |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165273A (en) * | 2020-02-26 | 2020-05-19 | 河南城建学院 | Culture method and culture medium for high-yield selenium-rich cordyceps militaris |
| CN111304095A (en) * | 2020-03-10 | 2020-06-19 | 秦小波 | Bolete hypha fertile culture medium and preparation method and application purification method thereof |
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| CN105018355A (en) * | 2015-08-21 | 2015-11-04 | 山东晨阳菌业有限公司 | Breeding method for hypsizigus marmoreus strains |
| CN107652014A (en) * | 2017-11-09 | 2018-02-02 | 成都市斯贝佳科技有限公司 | A kind of cultivation Bag Material only used true pleurotus cornucopiae |
| CN108276071A (en) * | 2017-01-06 | 2018-07-13 | 桂林洁宇环保科技有限责任公司 | Application of the sophora bud slag in true pleurotus cornucopiae cultivating material |
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2018
- 2018-09-14 CN CN201811074239.8A patent/CN109182450A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105018355A (en) * | 2015-08-21 | 2015-11-04 | 山东晨阳菌业有限公司 | Breeding method for hypsizigus marmoreus strains |
| CN108276071A (en) * | 2017-01-06 | 2018-07-13 | 桂林洁宇环保科技有限责任公司 | Application of the sophora bud slag in true pleurotus cornucopiae cultivating material |
| CN107652014A (en) * | 2017-11-09 | 2018-02-02 | 成都市斯贝佳科技有限公司 | A kind of cultivation Bag Material only used true pleurotus cornucopiae |
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| 刘明广 等: "真姬菇杂交菌株H11的选育", 《食用菌》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165273A (en) * | 2020-02-26 | 2020-05-19 | 河南城建学院 | Culture method and culture medium for high-yield selenium-rich cordyceps militaris |
| CN111165273B (en) * | 2020-02-26 | 2022-04-15 | 河南城建学院 | Culture method and culture medium for high-yield selenium-rich cordyceps militaris |
| CN111304095A (en) * | 2020-03-10 | 2020-06-19 | 秦小波 | Bolete hypha fertile culture medium and preparation method and application purification method thereof |
| CN111304095B (en) * | 2020-03-10 | 2022-03-22 | 秦小波 | Bolete hypha fertile culture medium and preparation method and application purification method thereof |
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Application publication date: 20190111 |