AU2021103049A4 - Saline-alkali tolerant Trichoderma viride Tv-1511 and bio-organic fertilizer and application thereof - Google Patents
Saline-alkali tolerant Trichoderma viride Tv-1511 and bio-organic fertilizer and application thereof Download PDFInfo
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Abstract
The present invention provides a saline-alkali tolerant Trichoderma viride Tv-1511 and a
bio-organic fertilizer and application thereof, belonging to the field of microbial technology.
By further in-depth studies on Trichoderma viride Tv-1511 screened early, it was found that
the Trichoderma viride Tv-1511 had good stress resistance, good adaptability to saline-alkali,
low-temperature and high-temperature environments, and an antagonistic effect on a variety
of pathogenic fungi. A solid fermentation product of the Trichoderma viride Tv-1511 can be
added to an organic fertilizer to prepare a Trichoderma bio-organic fertilizer suitable for
saline-alkali soil, which has good practical application value.
Description
Description
SALINE-ALKALI TOLERANT TRICHODERMA VIRIDE TV-1511 AND BIO-ORGANIC FERTILIZER AND APPLICATION THEREOF
Field of the Invention The present invention belongs to the field of microbial technology, and specifically relates to a saline-alkali tolerant Trichoderma viride Tv-1511 and a bio-organic fertilizer and application thereof.
Background of the Invention The information disclosed in the Background of the Invention is merely intended to increase the understanding on the general background of the present invention, and should not be construed as acknowledgment or hint in any form that the information constitutes the prior art known by those skilled in the art. Soil salinization is one of the main types of land degradation. It is an ecological problem that threatens the world, and is also one of the crises for human beings nowadays. According to statistics, saline-alkali soil in the world today has reached 1 billion hm2 , accounting for 7% of the total land area. In recent years, due to climatic factors and unreasonable development and utilization, the area of soil salinization is still expanding, which further threatens precious and scarce land resources. How to effectively use saline-alkali soil has become a hot issue all over the world. Bio-organic fertilizers are a type of fertilizers with the effects of both microbial fertilizers and organic fertilizers. The application of bio-organic fertilizers can not only promote plant growth, antagonize pathogenic fungi, increase crop yield and quality, but also improve soil, increase fertility, improve the ecological environment, etc. Therefore, the development of a suitable bio-organic fertilizer for saline-alkali soil areas is an effective way to accelerate the improvement on saline-alkali soil. The core of the bio-organic fertilizer is a microorganism with specific functions. According to the characteristics of saline-alkali soil, the screening of functional microorganisms that can tolerate saline-alkali environments is the key to determining the effectiveness of bio-organic fertilizers.
Summary of the Invention
In order to overcome the above technical problems, the present invention provides a
saline-alkali tolerant Trichoderma viride Tv-1511 and a bio-organic fertilizer and application
Description
thereof. By further in-depth studies on Trichoderma viride Tv-1511 screened early, it was
found that the Trichoderma viride Tv-1511 had good stress resistance, good adaptability to
saline-alkali, low-temperature and high-temperature environments, and an antagonistic effect
on a variety of pathogenic fungi. A solid fermentation product of the the Trichoderma viride
Tv-1511 can be further added to an organic fertilizer to prepare a Trichoderma bio-organic
fertilizer suitable for saline-alkali soil, which has good practical application value.
In order to achieve the above technical objectives, the technical solutions adopted by the
present invention are as follows:
The first aspect of the present invention provides an application of exogenous calcium in
improving the salt tolerance of Trichoderma viride Tv-1511 or a bio-organic fertilizer
containing Trichoderma viride Tv-1511.
The second aspect of the present invention provides a bio-organic fertilizer, the bio-organic
fertilizer at least including:
(A) the above-mentioned Trichoderma viride Tv-1511 or a solid fermentation product
thereof; and
(B) a calcium source.
A preparation method of the solid fermentation product is as follows: inoculating the
Trichoderma viride Tv-1511 to a medium containing mushroom residue, and culturing at
-30°C for 15-25 days to obtain the solid fermentation product of the Trichoderma viride
Tv-1511. The calcium source can not only effectively alleviate the growth inhibition of
Trichoderma viride Tv-1511 by salt stress, but also improve the salt tolerance of plants by
inhibiting the generation of reactive oxygen species. After the bio-organic fertilizer is applied
to saline-alkali soil, it can effectively increase the survival rate of Trichoderma viride
Tv-1511 and crops, and achieve the effects of promoting growth and increasing production.
The third aspect of the present invention provides an application of the above-mentioned
Trichoderma viride Tv-1511 and/or bio-organic fertilizer in any one or more of the following:
1) application in inhibiting pathogenic fungi;
2) application in preparation of pathogenic fungi inhibitors;
3) application in inhibiting plant diseases; and
4) application in preparation of plant disease inhibitors.
Description
The fourth aspect of the present invention provides an application of the above-mentioned
Trichoderma viride Tv-1511 and/or bio-organic fertilizer in promoting the increase of crop
production.
In the application, the application environment is a saline-alkali environment.
The beneficial technical effects of one or more of the above technical solutions are:
1) In agricultural production, Trichoderma inevitably faces some unfavorable environments,
such as high salt and high temperature, which directly affect the survival of Trichoderma. The
present invention evaluates the salt tolerance, alkali tolerance, low-temperature tolerance,
heat resistance and pathogenic fungi antagonism of Trichoderma viride Tv-1511, and found
that it has strong stress resistance and can adapt to different saline-alkali environments. The
Trichoderma viride Tv-1511 can achieve long-term colonization in saline-alkali soil, which is
conducive to the sustainable development of saline-alkali soil.
2) The above technical solution provides a Trichoderma bio-organic fertilizer suitable for
saline-alkali soil. The fertilizer is added with exogenous calcium on the basis of an original
bio-organic fertilizer. The exogenous calcium can not only effectively alleviate the growth
inhibition of Trichoderma viride Tv-1511 by salt stress, but also improve the salt tolerance of
plants by inhibiting the generation of reactive oxygen species. After the fertilizer is applied to
the saline-alkali soil, it can effectively increase the survival rate of Trichoderma viride
Tv-1511 and crops, and achieve the effects of promoting growth and increasing production.
3) Core field test sites were set up in the agricultural high-tech industry demonstration zone
of the Yellow River Delta, and field application effect studies of the Trichoderma bio-organic
fertilizer for saline-alkali soil were carried out under a variety of crops and soil conditions.
The Trichoderma bio-organic fertilizer of the present invention has a good growth-promoting
effect in saline-alkali soil, is suitable for large-area popularization and application, and has a
good development and application prospect.
Brief Description of the Drawings The accompanying drawings constituting a part of the present invention are used for providing a further understanding on the present invention, and the schematic embodiments of the present invention and the descriptions thereof are used for interpreting the present invention,
Description
rather than constituting improper limitations to the present invention. FIG. 1 illustrates an evaluation on salt tolerance of Trichoderma viride Tv-1511 in Example 1 of the present invention; FIG. 2 illustrates an evaluation on alkali resistance of Trichoderma viride Tv-1511 in Example 1 of the present invention; FIG. 3 illustrates an evaluation on low-temperature resistance of Trichoderma viride Tv-1511 in Example 1 of the present invention; FIG. 4 illustrates an evaluation on heat resistance of Trichoderma viride Tv-1511 in Experimental Example 1 of the present invention, where A is a culture situation map of Trichoderma viride Tv-1511 at different temperatures (28°C, 33°C, 35°C, 37C); B is a culture situation map of Trichoderma viride Tv-1511 thermally shocked at 37C, 40°C, 43°C, 46°C, and 49°C for 24 h and then inoculated to a PDA medium for culture in a 28°C incubator; FIG. 5 is a diagram of Trichoderma viride Tv-1511 antagonizing pathogenic fungi in Experimental Example 2 of the present invention; FIG. 6 illustrates the effect of exogenous calcium on the salt tolerance of Trichoderma viride Tv-1511 in Experimental Example 3 of the present invention.
Detailed Description of Embodiments
It should be pointed out that the following detailed descriptions are all exemplary and aim to
further illustrate the present invention. Unless otherwise specified, all technical and scientific
terms used in the descriptions have the same meanings generally understood by those of
ordinary skill in the art of the present invention. It should be noted that the terms used herein are merely for describing specific embodiments, but are not intended to limit exemplary embodiments according to the present invention. As used herein, unless otherwise clearly stated in the context, the singular form is also intended to include the plural form. In addition, it should also be understood that when the terms "include" and/or "comprise" are used in the Description, they indicate features, steps, operations, devices, components, and/or combinations thereof. It should be understood that the protection scope of the present invention is not limited to the following specific implementation schemes. It should also be understood that the terms used in the examples of the present invention are used to describe specific implementation schemes, and are not to limit the protection scope of the present invention. As mentioned earlier, the development of a suitable bio-organic fertilizer for saline-alkali soil
Description
areas is an effective way to accelerate the improvement on saline-alkali soil. The core of the bio-organic fertilizer is microorganisms with specific functions. According to the characteristics of saline-alkali soil, the screening of functional microorganisms that can tolerate saline-alkali environments is the key to determining the effectiveness of the bio-organic fertilizer.
The present invention has been confirmed through research that exogenous calcium can
significantly enhance the salt tolerance of Trichoderma viride Tv-1511. Therefore, in a
typical embodiment of the present invention, an application of exogenous calcium in
improving the salt tolerance of Trichoderma viride Tv-1511 or a bio-organic fertilizer
containing Trichoderma viride Tv-1511 is provided.
The exogenous calcium is calcium chloride or superphosphate; further, the concentration of
the calcium chloride or superphosphate is controlled to be 0.1-1 mM, preferably 0.5 mM.
In another specific embodiment of the present invention, a bio-organic fertilizer is provided,
the bio-organic fertilizer at least including:
(A) the above-mentioned Trichoderma viride Tv-1511 or a solid fermentation product
thereof; and
(B) a calcium source.
In another specific embodiment of the present invention, a preparation method of the solid
fermentation product is as follows: inoculating the Trichoderma viride Tv-1511 to a medium
containing mushroom residue, and culturing at 20-30°C for 15-25 days to obtain the solid
fermentation product of the Trichoderma viride Tv-1511. The calcium source can not only
effectively alleviate the growth inhibition of Trichoderma viride Tv-1511 by salt stress, but
also improve the salt tolerance of plants by inhibiting the generation of reactive oxygen
species. After the bio-organic fertilizer is applied to saline-alkali soil, it can effectively
increase the survival rate of Trichoderma viride Tv-1511 and crops, and achieve the effects of
promoting growth and increasing production.
In another specific embodiment of the present invention, the calcium source is specifically a
calcium salt, more preferably superphosphate or calcium chloride.
In another specific embodiment of the present invention, the added amount of the solid
fermentation product is 1-10% of the dry weight of the bio-organic fertilizer, preferably 5%.
Description
In another specific embodiment of the present invention, the bio-organic fertilizer includes a
solid fermentation product of Trichoderma viride Tv-1511, a calcium source and an organic
fertilizer.
In another specific embodiment of the present invention, the organic fertilizer is provided by
Jinxing (Rizhao) Agricultural Technology Co., Ltd., with water content <30%, organic matter
>45%, total nitrogen 4%, total phosphorus (P205) 4%, total potassium (K20) 2%, zinc sulfate
(ZnSO4) 0.05%, and free amino acid 1%.
In another specific embodiment of the present invention, an application of the
above-mentioned Trichoderma viride Tv-1511 and/or bio-organic fertilizer in any one or
more of the following is provided:
1) application in inhibiting pathogenic fungi;
2) application in preparation of pathogenic fungi inhibitors;
3) application in inhibiting plant diseases; and
4) application in preparation of plant disease inhibitors.
In another specific embodiment of the present invention, the pathogenic fungi may be all or
part of the following pathogenic fungi: Fusarium oxysporum, Fusarium prolferatum and
Scleritium rolfsii.
In another specific embodiment of the present invention, the diseases may be all or part of the
following diseases: fusarium wilt, ear rot and Sclerotium rolfsii.
In another specific embodiment of the present invention, an application of the
above-mentioned Trichoderma viride Tv-1511 and/or bio-organic fertilizer in promoting the
increase of crop production is provided.
In another specific embodiment of the present invention, the crops are corn and wheat,
further, the wheat is Ji Mai 22, and the corn is Hua Nong 138.
In another specific embodiment of the present invention, in the application, the application
environment is a saline-alkali environment.
It should be noted that the Trichoderma viride Tv-1511 was collected in the China General
Microbiological Culture Collection Center (Address: Institute of Microbiology, Chinese
Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing) on
December 20, 2018, with Collection Number: CGMCC NO.16800. This strain has been
Description
disclosed in the patent No. ZL 201910626525.9.
The technical solutions of the present invention will be described below through specific
examples. The raw materials used in the following examples are all commercially available.
Example 1 Evaluation on stress resistance of Trichoderma viride Tv1511
1.1 Salt tolerance
Trichoderma viride Tv-1511 clumps were inoculated to PDAs with different salt
concentrations (CK, 0.5%, 1%, 1.5%, 2%, 3%, 4%, 5% NaCl) and cultured at 28°C, radii of
Trichoderma growth circles were measured and the shape and size of the growth circles were
scanned every day, and the fresh and dry weights of the bacteria were measured.
The results of salt tolerance experiments showed that Tv-1511 can maintain normal growth
under 0.5% NaCl treatment; Tv-1511 also maintained a high biomass under 1% and 1.5%
NaCl treatments; and when the NaCl concentration reached 5%, Tv-1511 still maintained
certain activity (FIG. 1).
1.2 Alkali resistance
The pH value was adjusted to 4, 5, 6, 7, 8, and 9 with HCl and NaOH, Trichoderma viride
Tv-1511 clumps were inoculated and cultured at 28°C, and the shape and size of Trichoderma
growth circles were observed. The results showed that the optimum growth pH value for the
Trichoderma viride Tv1511 was 6-7; and the Trichoderma viride Tv1511 can also grow on a
medium with a pH value of 9 (FIG. 2), and had strong alkali resistance.
1.3 Low-temperature resistance
The PDAs inoculated with the Trichoderma viride Tv-1511 were respectively cultured in
incubators at different temperatures (8°C, 13°C, 18°C, 23°C, 28°C, 33°C, 35°C, 37C), and
the shape and size of Trichoderma growth circles were observed. The results showed that the
optimum growth temperature of the Trichoderma viride Tv1511 was 28°C; and when the
temperature reached 13°C, the Tv-1511 still maintained certain activity (FIG. 3) and had
strong low-temperature resistance.
1.4 Heat resistance
The PDAs inoculated with the Trichoderma viride Tv-1511 were respectively cultured in
Description
incubators at different temperatures (28°C, 33°C, 35°C, 37C), and the shape and size of
Trichoderma growth circles were observed. The results showed that the Trichoderma viride
Tv1511 still maintained certain activity when the temperature reached 33°C and 35°C, and
had good heat resistance (FIG. 4, A).
Furthermore, a spore liquid of the Trichoderma viride Tv-1511 was thermally shocked at
37°C, 40°C, 43°C, 46°C and 49°C for 24 h, and then inoculated to a PDA medium for culture
in a 28°C incubator, and the shape and size of Trichoderma growth circles were observed.
The results showed that the Trichoderma viride Tv1511 can recover growth after thermal
shock at 46°C for 24 h, and had strong high-temperature resistance (FIG. 4, B).
Example 2: Application of Trichoderma viride Tv-1511 in antagonizing pathogenic fungi
Three plant pathogenic fungi were selected, including Fusarium oxysporum, Fusarium
proliferatum and Scleritium rofsii. Clumps with a diameter of 5 mm were taken with a
puncher at edges of colonies, the plant pathogenic fungi and the Trichoderma viride Tv-1511
were respectively inoculated at symmetrical positions 1.5 cm away from two side dish edges
on a diameter line, and PDAs not inoculated with the Trichoderma but inoculated with only
the pathogenic fungi were used as a control. The clumps were cultured in 28°C thermostatic
incubators, the fungal confrontation was photographed, and the inhibitory rate was calculated
according to the following formula.
Inhibitory rate (%) = (control pathogenic fungi colony radius-treated pathogenic fungi colony
radius)/control pathogenic fungi colony radius x 100%.
The results showed that the Trichoderma viride Tv-1511 had varying degrees of inhibition
effects on the Fusarium oxysporum, the Fusariumproliferatum and the Scleritium rolfsii. At
the initial stage of inoculation, the Trichoderma viride Tv-1511 grew normally, and the
pathogenic fungi were inhibited to a certain extent; on day 4 after inoculation, the
Trichoderma viride Tv-1511 was in contact with the pathogenic fungi, and the growth of the
pathogenic fungi was significantly inhibited; on day 8 after inoculation, the Trichoderma
viride Tv-1511 covered or invaded the fungal colonies and formed a large number of green
spores to prevent further growth of the pathogenic fungi. The inhibitory rates against the
Fusarium oxysporum, the Fusariumproliferatum and the Scleritium rofsii were respectively
Description
71.9%, 66.6% and 57.2% (See the table below and FIG. 5). Therefore, the Trichoderma viride
Tv-1511 had an antagonistic effect on the pathogenic fungi such as the Fusariumoxysporum,
the Fusariumproiferatum and the Scleritium rolfsii, and can be used for biological control of
soil-borne diseases.
Scleritium Fusarium FusariuR rolfsii proliferatum oxysporum Treated pathogenic 2.51 2.42 2.20 1.28 1.46 1.21 1.63 1.51 1.57 fungi radius/cm Control pathogenic 8.45 8.45 8.45 3.88 399 3.97 375 3.88 3.41 fungi radius/cm Inhibitory rate ° 70.3 71.3 74.0 67.0 63.4 69.5 56.5 61.1 54.0 Average inhibitory rate/% 71.9 66.6 57.2
Example 3: Application of exogenous calcium in improving the salt tolerance of
Trichoderma viride Tv-1511
This example was carried out in the Industrial Microbiology Laboratory of the Institute of
Biology, Shandong Academy of Sciences. Take a normal PDA medium as a control, a PDA
medium added with 0.5 mM CaCl2 as treatment 1 (TI), a PDA medium added with 300 mM
NaCl as treatment 2 (T2), and a PDA medium added with 300 mM NaCl and 0.5 mM CaCl2
as treatment 3 (T3), with three replicates for each treatment. Trichoderma viride Tv-1511
clumps were inoculated and cultured at 28°C thermostatic incubators, radii of Trichoderma
growth circles were measured and the shape and size of the growth circles were scanned
every day.
The results showed that 0.5 mM CaCl2 did not have a significant promotion effect on the
growth of Tv-1511 under normal conditions. Under salt stress, the growth of the Trichoderma
viride Tv-1511 was inhibited. After treated with 300 mM NaCI for 72 h, the colony radius of
Tv-1511 was 17.31 mm shorter than that of the control group, a decrease of 36.2%; after 0.5
mM CaCl2 was added, the inhibition on the growth of Tv-1511 hyphae was significantly
alleviated; after 72 hours of treatment, the colony radius of the T3 group (300 mM NaC+0.5
mM CaC2) was 1.67 times that of the T2 group (300 mM NaCl) (FIG. 6). Therefore, the
Description
exogenous calcium can significantly enhance the salt tolerance of the Trichoderma viride
Tv-1511.
Example 4: Field demonstration of a Trichoderma bio-organic fertilizer in saline-alkali
soil
4.1. Test materials
Test microorganism: Trichoderma viride Tv-1511.
Test crops: wheat (Ji Mai 22), bred by Crop Research Institute, Shandong Academy of
Agricultural Sciences. Corn (Hua Nong 138), purchased from Beijing Huanong Weiye Seed
Technology Co., Ltd.
Trichoderma bio-organic fertilizer for test: provided by Jinxing (Rizhao) Agricultural
Technology Co., Ltd., with water content <30%, organic matter > 4 5 %, total nitrogen 4%,
total phosphorus (P205) 4%, total potassium (K20) 2%, zinc sulfate (ZnSO4) 0.05%, and free
amino acid 1%. A Trichoderma solid fermentation product was added in an amount of 5% of
the dry weight of the organic fertilizer to prepare a bio-organic fertilizer.
Trichoderma solid fermentation product: the Trichoderma viride Tv-1511 was inoculated in
an amount of 6% to a medium containing mushroom residue, and cultured at 30°C for 20
days.
Trichoderma bio-organic fertilizer for test saline-alkali soil: provided by Jinxing (Rizhao)
Agricultural Technology Co., Ltd., a certain proportion of superphosphate was added on the
basis of the Trichoderma bio-organic fertilizer.
4.2. Experimental design
The field demonstration experiment was carried out in the Yellow River Delta Agricultural
High-Tech Industry Demonstration Zone in Shandong Province. Soil had a low organic
matter content of 0.6 1 %-1.01%, and contained high potassium, low nitrogen and low
phosphorus; the pH value of the soil was relatively alkaline, higher than 8.0; and the salt
content was 0.3%-0.6%, so the soil was moderately severe saline-alkali soil. The
experimental design was as follows: a control group (CK) was conventionally fertilized by
farmers; the Trichoderma bio-organic fertilizer was applied to treatment 1 (TI); the
Trichoderma bio-organic fertilizer and superphosphate (its added amount was 19.95 kg/mu
for corn and 28.50 kg/mu for wheat) were applied to treatment 2 (T2), with 3 replicates for
Description
each treatment, routine management, and weighing after maturity.
4.3. Results
The effects of the Trichoderma bio-organic fertilizer on the thousand-grain weight and yield
of saline-alkali soil wheat were shown in Table 1. The thousand-grain weights of wheat after
TI (applied with the Trichoderma bio-organic fertilizer) and T2 (applied with the
Trichoderma bio-organic fertilizer added with superphosphate) were respectively 1.03 times
and 1.04 times that of CK, wherein the effect of the T2 treatment on the thousand-grain
weight of wheat reached a significant level (P<0.05).
Compared with the CK (conventional fertilization) treatment, the TI (applied with the
Trichoderma bio-organic fertilizer) treatment increased the yield of wheat by 42.01 kg per
mu, an increase of 11.90%; and the T2 (applied with the Trichoderma bio-organic fertilizer
added with superphosphate) treatment increased the yield of wheat by 66.91 kg per mu, an
increase of 18.95%. The two treatments reached a significant level of difference (P<0.05).
Therefore, the application of the Trichoderma bio-organic fertilizer and the addition of
superphosphate on the basis of the Trichoderma bio-organic fertilizer can significantly
increase the yield of wheat, and the fertilization of superphosphate added on the basis of the
Trichoderma bio-organic fertilizer had the most obvious effect on yield increase.
Table 1 Effects of Trichoderma bio-organic fertilizer for saline-alkali soil on thousand-grain
weight and yield of wheat
Significant Significant Thousand-grain Thousandgri difference Yield difference Treatment weight (Kg/mu) (g) P<0.05 P<0.05
CK 38.96±1.75 b 353.03+19.44 b TI 40.22+0.47 ab 395.04+5.71 a T2 40.70+1.55 a 419.94+9.54 a
The effects of the Trichoderma bio-organic fertilizer on the hundred-grain weight and yield of
saline-alkali soil corn were shown in Table 2. Compared with the CK (conventional
fertilization) treatment, the hundred-grain weights of corn treated by TI (applied with the
Trichoderma bio-organic fertilizer) and T2 (applied with the Trichoderma bio-organic
fertilizer added with superphosphate) increased by 5.54% and 6.62%, respectively. The effect
Description
of the T2 treatment on the hundred-grain weight of corn reached a significant level (P<
0.05). Compared with the CK (conventional fertilization) treatment, the TI (applied with the
Trichoderma bio-organic fertilizer) treatment increased the yield of corn by 61.94 kg per mu,
an increase of 15.48%; and the T2 (applied with the Trichoderma bio-organic fertilizer added
with superphosphate) treatment increased the yield of corn by 126.19 kg per mu, an increase
of 31.54%. The two treatments reached a significant level of difference (P<0.05) over the
CK, and the T2 treatment also reached a significant level of difference (P<0.05) over the TI
treatment. Therefore, the application of the Trichoderma bio-organic fertilizer and the
addition of superphosphate on the basis of the Trichoderma bio-organic fertilizer can
significantly increase the yield of corn, and the fertilization of superphosphate added on the
basis of the Trichoderma bio-organic fertilizer had the most obvious effect on yield increase.
Table 2 Effects of Trichoderma bio-organic fertilizer for saline-alkali soil on hundred-grain
weight and yield of corn
Significant Significant Hundred-grain Hundredgri difference Yield difference Treatment weight (Kg/mu) (g) P<0.05 P<0.05
CK 26.73+0.93 b 400.16+24.83 c T1 28.21+0.59 ab 462.10+19.08 b T2 28.50+0.83 a 526.35+20.71 a
Finally, it should be noted that the above descriptions are only preferred embodiments of the
present invention and are not intended to limit the present invention. Although the present
invention is described in detail with reference to the foregoing embodiments, the technical
solutions described in the foregoing embodiments can still be modified or some of them can
be equivalently substituted by those skilled in the art. Any modification, equivalent
substitution or improvement made within the spirit and principle of the present invention
shall fall into the protection scope of the present invention. The specific embodiments of the
present invention are described above, but are not intended to limit the protection scope of the
present invention. Those skilled in the art should understand that various modifications or
variations that can be made by those skilled in the art without contributing creative labor are
Description
still within the protection scope of the present invention on the basis of the technical solution
of the present invention.
Claims (6)
1. An application of exogenous calcium in improving the salt tolerance of Trichoderma viride
Tv-1511 or a bio-organic fertilizer containing Trichoderma viride Tv-1511.
2. The application according to claim 1, wherein the exogenous calcium is calcium chloride
or superphosphate; and
preferably, the concentration of the exogenous calcium is controlled to be 0.1-1 mM, more
preferably 0.5 mM.
3. An application of Trichoderma viride Tv-1511 in any one or more of the following:
1) application in inhibiting pathogenic fungi;
2) application in preparation of pathogenic fungi inhibitors;
3) application in inhibiting plant diseases; and
4) application in preparation of plant disease inhibitors.
4. The application according to claim 3, wherein the pathogenic fungi are all or part of the
following pathogenic fungi: Fusarium oxysporum, Fusarium prolferatum and Scleritium
rolfsii.
5. The application according to claim 3, wherein the diseases are all or part of the following
diseases: fusarium wilt, ear rot and Sclerotium rolfsii.
6. An application of Trichoderma viride Tv-1511 in promoting crop production;
preferably, the crops are corn and wheat; and
preferably, in the application, the application environment is a saline-alkali environment.
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| CN202011428492.6A CN112410232B (en) | 2020-12-09 | 2020-12-09 | Saline-alkali-tolerant trichoderma viride, biological organic fertilizer and application thereof |
| CN2020114284926 | 2020-12-09 |
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| AU2021103049A4 true AU2021103049A4 (en) | 2021-07-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107327A (en) * | 2021-12-08 | 2022-03-01 | 山东省科学院生物研究所 | Trichoderma viride high-temperature stress response key enzyme gene TvHSP70, recombinant expression vector, engineering bacteria and application thereof |
| CN115197957A (en) * | 2022-05-16 | 2022-10-18 | 南京农业大学 | Gene fragment for improving crop straw decomposition of trichoderma guizhouense NJAU4742 in adversity and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110218659B (en) * | 2019-07-11 | 2020-04-07 | 山东省科学院生物研究所 | Trichoderma viride, microbial inoculum, biological fertilizer and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107327A (en) * | 2021-12-08 | 2022-03-01 | 山东省科学院生物研究所 | Trichoderma viride high-temperature stress response key enzyme gene TvHSP70, recombinant expression vector, engineering bacteria and application thereof |
| CN114107327B (en) * | 2021-12-08 | 2024-02-23 | 山东省科学院生物研究所 | Trichoderma viride high-temperature stress response key enzyme gene TvHSP70, recombinant expression vector, engineering bacteria and application thereof |
| CN115197957A (en) * | 2022-05-16 | 2022-10-18 | 南京农业大学 | Gene fragment for improving crop straw decomposition of trichoderma guizhouense NJAU4742 in adversity and application thereof |
| CN115197957B (en) * | 2022-05-16 | 2023-10-10 | 南京农业大学 | Gene segment for improving decomposition of trichoderma guizhou NJAU4742 into crop straws under adverse conditions and application thereof |
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| CN112410232A (en) | 2021-02-26 |
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