CN112410232A - Saline-alkali-tolerant trichoderma viride, biological organic fertilizer and application thereof - Google Patents

Saline-alkali-tolerant trichoderma viride, biological organic fertilizer and application thereof Download PDF

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CN112410232A
CN112410232A CN202011428492.6A CN202011428492A CN112410232A CN 112410232 A CN112410232 A CN 112410232A CN 202011428492 A CN202011428492 A CN 202011428492A CN 112410232 A CN112410232 A CN 112410232A
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trichoderma viride
organic fertilizer
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trichoderma
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黄艳华
郭凯
李哲
郝永任
郑泽慧
刘奎祝
张豪
隋永辉
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Venus Rizhao Agricultural Science And Technology Development Co ltd
Biology Institute of Shandong Academy of Sciences
Golden Star Rizhao Agricultural Science and Technology Development Co Ltd
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Abstract

The invention provides saline-alkali tolerant trichoderma viride, a biological organic fertilizer and application thereof, and belongs to the technical field of microorganisms. According to the invention, through further and in-depth research on a Trichoderma viride Tv-1511 strain obtained by early screening, the Trichoderma viride has good stress resistance, good adaptability to saline-alkali, low-temperature and high-temperature environments and antagonism to various pathogenic fungi, and the Trichoderma bio-organic fertilizer suitable for saline-alkali soil can be prepared by adding the solid fermentation product of the Trichoderma bio-organic fertilizer into an organic fertilizer, so that the Trichoderma bio-organic fertilizer has good practical application value.

Description

Saline-alkali-tolerant trichoderma viride, biological organic fertilizer and application thereof
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to saline-alkali tolerant trichoderma viride, a biological organic fertilizer and application thereof.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
Soil salinization is one of the main types of land degradation, is an ecological problem threatening the world, and is also one of the crises facing human beings today. According to statistics, the saline-alkali soil in the world reaches 10 hundred million hm2, and accounts for 7 percent of the total land area. In recent years, the salinized area of soil is still continuously expanded due to climate factors and unreasonable development and utilization, and further the precious and scarce land resources are threatened. How to effectively utilize saline-alkali soil has become a hot problem all over the world.
The biological organic fertilizer is a fertilizer with the effects of both microbial fertilizer and organic fertilizer. The biological organic fertilizer is applied, so that the growth of plants can be promoted, pathogenic bacteria can be antagonized, the yield and the quality of crops can be increased, the soil can be improved, the fertility can be improved, the ecological environment can be improved and the like. Therefore, the development of the bio-organic fertilizer suitable for saline-alkali areas is an effective way for accelerating the improvement of saline-alkali soil. The core of the biological organic fertilizer is the microorganism with specific function. Aiming at the characteristics of saline-alkali soil, screening functional microorganisms capable of tolerating saline-alkali environment is a key for determining the effectiveness of the biological organic fertilizer.
Disclosure of Invention
In order to overcome the technical problems, the invention provides a saline-alkali tolerant Trichoderma viride, a biological organic fertilizer thereof and application thereof, and further intensive research on a Trichoderma viride Tv-1511 obtained by early screening discovers that the Trichoderma viride has good stress resistance, has good adaptability to saline-alkali, low-temperature and high-temperature environments, has antagonism on various pathogenic fungi, can be prepared into the Trichoderma type biological organic fertilizer suitable for saline-alkali soil by further adding a solid fermentation product of the Trichoderma type biological organic fertilizer into the organic fertilizer, and has good value of practical application.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:
in a first aspect of the invention, there is provided the use of exogenous calcium to improve the salt tolerance of Trichoderma viride (Trichoderma viride) Tv-1511 or a bioorganic fertilizer containing Trichoderma viride (Trichoderma viride) Tv-1511.
In a second aspect of the present invention, there is provided a bio-organic fertilizer, which at least comprises:
(a) trichoderma viride Tv-1511 or a solid fermentation product thereof;
(b) a source of calcium.
The preparation method of the solid fermentation product comprises the following steps: inoculating Trichoderma viride Tv-1511 into the culture medium containing mushroom residue, and culturing at 20-30 deg.C for 15-25 days to obtain Trichoderma viride solid fermented product. The calcium source can effectively relieve the growth inhibition of salt stress on the trichoderma viride Tv-1511, and can also improve the salt tolerance of plants by inhibiting the generation of active oxygen substances. After the biological organic fertilizer is applied to saline-alkali soil, the survival rate of trichoderma viride Tv-1511 and crops can be effectively improved, and the effects of promoting growth and increasing yield are achieved.
In a third aspect of the present invention, there is provided the use of Trichoderma viride (Trichoderma viride) Tv-1511 and/or a bio-organic fertilizer as described above in any one or more of:
1) the application in inhibiting pathogenic bacteria;
2) the application in the preparation of pathogenic bacteria inhibitor;
3) the application in inhibiting plant diseases;
4) application in preparing plant disease inhibitor.
In a fourth aspect of the present invention, there is provided an application of Trichoderma viride (Trichoderma viride) Tv-1511 and/or a bio-organic fertilizer in promoting crop yield increase.
In the application, the application environment is a saline-alkali environment.
The beneficial technical effects of one or more technical schemes are as follows:
1) in agricultural production, trichoderma inevitably faces some adverse environments, such as high salt, high temperature and the like, and directly influences the survival of trichoderma. The invention evaluates the salt tolerance, alkali tolerance, low temperature resistance, heat resistance, pathogenic bacterium antagonism and the like of the trichoderma viride Tv-1511, and finds that the trichoderma viride Tv-1511 has stronger stress resistance and can adapt to different saline-alkali environments. The trichoderma viride Tv-1511 can realize long-term colonization in saline-alkali soil, and is beneficial to sustainable development of the saline-alkali soil.
2) The trichoderma bio-organic fertilizer applicable to saline-alkali soil is provided by the technical scheme. The fertilizer is added with exogenous calcium on the basis of the original biological organic fertilizer. The exogenous calcium can effectively relieve the growth inhibition of salt stress on the trichoderma viride Tv-1511, and can improve the salt tolerance of plants by inhibiting the generation of active oxygen substances. After the fertilizer is applied to saline-alkali soil, the survival rate of trichoderma viride Tv-1511 and crops can be effectively improved, and the effects of promoting growth and increasing yield are achieved.
3) Core field test points are arranged in a high and new technology industry demonstration area of yellow river delta agriculture, and field application effect research of the trichoderma bio-organic fertilizer in the saline-alkali soil is developed under various crop and soil conditions. The trichoderma bio-organic fertilizer has good growth promoting effect in saline-alkali soil, is suitable for large-area popularization and application, and has good development and application prospects.
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The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is an evaluation of salt tolerance of Trichoderma viride Tv-1511 in example 1 of the present invention;
FIG. 2 is an evaluation of alkali resistance of Trichoderma viride Tv-1511 in example 1 of the present invention;
FIG. 3 is a diagram showing the evaluation of the low temperature resistance of Trichoderma viride Tv-1511 in example 1 of the present invention;
FIG. 4 is a heat resistance evaluation of Trichoderma viride Tv-1511 in Experimental example 1 of the present invention; wherein A is a graph of the culture condition of Trichoderma viride Tv-1511 at different temperatures (28 deg.C, 33 deg.C, 35 deg.C, 37 deg.C); b is a graph of the condition that trichoderma viride Tv-1511 is thermally shocked for 24 hours at the temperature of 37 ℃, 40 ℃, 43 ℃, 46 ℃ and 49 ℃, then inoculated on PDA culture medium and placed in an incubator at 28 ℃.
FIG. 5 is a diagram showing the antagonistic action of pathogenic fungi by Trichoderma viride Tv-1511 in Experimental example 2 of the present invention;
FIG. 6 is a graph showing the effect of exogenous calcium on salt tolerance of Trichoderma viride Tv-1511 in Experimental example 3 of the present invention.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise. It is to be understood that the scope of the invention is not to be limited to the specific embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.
As mentioned above, the development of the bio-organic fertilizer suitable for saline-alkali areas is an effective way for accelerating the improvement of saline-alkali soil. The core of the biological organic fertilizer is the microorganism with specific function. Aiming at the characteristics of saline-alkali soil, screening functional microorganisms capable of tolerating saline-alkali environment is a key for determining the effectiveness of the biological organic fertilizer.
The research proves that the exogenous calcium can obviously enhance the salt tolerance of the Trichoderma viride Tv-1511. Accordingly, in an exemplary embodiment of the invention, use of exogenous calcium to increase the salt tolerance of the aforementioned Trichoderma viride (Tv-1511) or a bioorganic fertilizer comprising Trichoderma viride (Tv-1511) is provided.
The exogenous calcium is calcium chloride or calcium superphosphate; further, the concentration of the calcium chloride or the calcium superphosphate is controlled to be 0.1-1 mM, and preferably 0.5 mM.
In another embodiment of the present invention, there is provided a bio-organic fertilizer, which at least comprises:
(a) trichoderma viride Tv-1511 or a solid fermentation product thereof;
(b) a source of calcium.
In another embodiment of the present invention, the method for preparing the solid fermentation product comprises: inoculating Trichoderma viride Tv-1511 into the culture medium containing mushroom residue, and culturing at 20-30 deg.C for 15-25 days to obtain Trichoderma viride solid fermented product. The calcium source can effectively relieve the growth inhibition of salt stress on the trichoderma viride Tv-1511, and can also improve the salt tolerance of plants by inhibiting the generation of active oxygen substances. After the biological organic fertilizer is applied to saline-alkali soil, the survival rate of trichoderma viride Tv-1511 and crops can be effectively improved, and the effects of promoting growth and increasing yield are achieved.
In a further embodiment of the invention, the calcium source is in particular a calcium salt, more preferably calcium superphosphate or calcium chloride.
In another embodiment of the invention, the addition amount of the solid fermentation product is 1-10% of the dry weight of the bio-organic fertilizer, and preferably 5%.
In yet another embodiment of the present invention, the bio-organic fertilizer comprises a solid fermentation product of Trichoderma viride (Trichoderma viride) Tv-1511, a calcium source and an organic fertilizer.
In another embodiment of the invention, the organic fertilizer is provided by Venus (sunshine) agricultural science and technology Limited, the water content is less than or equal to 30%, the organic matter is more than or equal to 45%, the total nitrogen is 4%, and the total phosphorus (P) is2O5) 4% total potassium (K)2O) 2%, zinc sulfate (ZnSO)4) 0.05% and 1% of free Amino Acid (Amino Acid).
In another embodiment of the present invention, there is provided a use of Trichoderma viride (Trichoderma viride) Tv-1511 and/or a bio-organic fertilizer as described above in any one or more of:
1) the application in inhibiting pathogenic bacteria;
2) the application in the preparation of pathogenic bacteria inhibitor;
3) the application in inhibiting plant diseases;
4) application in preparing plant disease inhibitor.
In another embodiment of the present invention, the pathogenic bacteria may be all or part of the following pathogenic bacteria: fusarium oxysporum (Fusarium oxysporum), Fusarium proliferatum (Fusarium proliferatum), and Sclerotinium rolfsii (Sclerotinium rolfsii).
In another embodiment of the present invention, the disease may be all or part of the following diseases: blight, ear rot and southern blight.
In another embodiment of the present invention, there is provided the use of Trichoderma viride (Trichoderma viride) Tv-1511 and/or a bio-organic fertilizer as described above for promoting crop yield increase.
In another embodiment of the present invention, the crops are corn and wheat, further, the wheat is jimai 22, and the corn is huanong 138.
In another embodiment of the present invention, the application environment is saline-alkali environment.
It should be noted that, in the above, Trichoderma viride (Trichoderma viride) Tv-1511 has been deposited in the general microbiological culture collection of the china committee for culture collection of microorganisms at 20/12 in 2018, with the deposition addresses: the biological preservation number of the institute of microbiology of the Chinese academy of sciences, No. 3 Xilu-1 Beijing, Chaoyang, and the area of the rising Yang is: CGMCC NO. 16800. The strain is disclosed in patent No. ZL 201910626525.9.
The technical solution of the present invention will be described below with specific examples. The starting materials used in the following examples are all commercially available.
Example 1 evaluation of Trichoderma viride Tv1511 stress resistance
1.1 salt tolerance
Inoculating Trichoderma viride Tv-1511 strain blocks on plates with different salt concentrations (CK, 0.5%, 1%, 1.5%, 2%, 3%, 4%, 5% NaCl), culturing at 28 deg.C, measuring radius of Trichoderma growth circle, scanning shape and size of growth circle every day, and measuring fresh weight and dry weight of strain.
The results of salt tolerance experiments show that: under the treatment of 0.5% NaCl, the Tv-1511 can keep normal growth; tv-1511 also maintained higher biomass at 1% and 1.5% NaCl treatment; when the NaCl concentration reached 5%, the activity of Tv-1511 remained constant (FIG. 1).
1.2 alkali resistance
Adjusting pH to 4, 5, 6, 7, 8 and 9 with HCl and NaOH, inoculating Trichoderma viride Tv-1511 strain block, culturing at 28 deg.C, and observing the shape and size of Trichoderma growth circle. The results show that: the optimum growth pH value of the trichoderma viride Tv1511 is 6-7; can also grow on the culture medium with pH 9 (figure 2), and has strong alkali resistance.
1.3 Low temperature resistance
The PDA plates inoculated with the Tv-1511 strain blocks of the trichoderma viride are respectively placed in incubators with different temperatures (8 ℃, 13 ℃, 18 ℃, 23 ℃, 28 ℃, 33 ℃, 35 ℃ and 37 ℃) for culture, and the shape and the size of the trichoderma growth circle are observed. The results show that: the optimal growth temperature of trichoderma viride Tv1511 is 28 ℃; when the temperature reaches 13 ℃, Tv-1511 still keeps certain activity (figure 3), and has strong low temperature resistance.
1.4 Heat resistance
The PDA plates inoculated with the Tv-1511 strain blocks of the trichoderma viride are respectively placed in incubators with different temperatures (28 ℃, 33 ℃, 35 ℃ and 37 ℃) for culture, and the shape and the size of the trichoderma growth circle are observed. The results show that: the Trichoderma viride Tv1511 still keeps certain activity when the temperature reaches 33 ℃ and 35 ℃, and has better heat resistance (figure 4, A).
Further, the Trichoderma viride Tv-1511 spore liquid is respectively placed at the temperature of 37 ℃, 40 ℃, 43 ℃, 46 ℃ and 49 ℃ for heat shock for 24 hours, then inoculated on a PDA culture medium and placed in a 28 ℃ incubator for culture, and the shape and the size of the Trichoderma growth circle are observed. The results show that: the trichoderma viride Tv1511 can recover growth after heat shock for 24h at 46 ℃, and has strong high temperature resistance (figure 4, B).
Example 2: application of trichoderma viride Tv-1511 in antagonizing pathogenic fungi
Three plant pathogenic fungi are selected, including Fusarium oxysporum (Fusarium oxysporum), Fusarium proliferatum (Fusarium proliferatum) and Sclerithium rolfsii. A puncher is used for taking a fungus block with the diameter of 5mm from the edge of a bacterial colony, plant pathogenic fungi and trichoderma viride Tv-1511 are respectively inoculated at symmetrical positions 1.5cm away from the edges of dishes on two sides on a diameter line, and meanwhile, plates which are not inoculated with the trichoderma viride and only inoculated with the pathogenic fungi are used as controls. Culturing in a constant-temperature incubator at 28 ℃, photographing and recording the confronting conditions of fungi, and calculating the bacteriostasis rate according to the following formula.
The bacteriostatic ratio (%) - (control pathogen colony radius-treatment pathogen colony radius)/control pathogen colony radius × 100%.
The results show that the trichoderma viride Tv-1511 has different degrees of inhibition effects on fusarium oxysporum, fusarium laminarinum and southern blight. At the initial stage of inoculation, trichoderma viride Tv-1511 grows normally, and pathogenic bacteria are inhibited to a certain degree; after 4 days of inoculation, the trichoderma viride Tv-1511 is contacted with pathogenic bacteria, and the growth of the pathogenic bacteria is obviously inhibited; after 8 days of inoculation, the trichoderma viride Tv-1511 covers or invades pathogenic bacteria colonies, forms a large amount of green spores, prevents further growth of pathogenic bacteria, and has bacteriostasis rates of 71.9%, 66.6% and 57.2% for sclerotinia sclerotiorum, fusarium stratiotes and fusarium oxysporum respectively (see the following table and figure 5). Therefore, the trichoderma viride Tv-1511 has antagonistic action on pathogenic bacteria such as fusarium oxysporum, fusarium laminarum and southern blight, and can biologically control soil-borne fungal diseases.
Figure BDA0002825731430000091
Example 3: application of exogenous calcium in improving salt tolerance of trichoderma viride Tv-1511
This example was carried out in the laboratory of microorganisms of the institute of sciences, Shandong province. Normal PDA medium was used as a control to add 0.5mM CaCl2PDA medium (1) (T1) and PDA medium (2) (T2) supplemented with 300mM NaCl and 0.5mM CaCl2PDA medium of (3) was treatment 3(T3), with 3 replicates per treatment set up. Inoculating a Trichoderma viride Tv-1511 strain block, culturing in a constant-temperature incubator at 28 ℃, measuring the radius of a Trichoderma growth ring every day, and scanning the shape and size of the growth ring.
The results showed 0.5mM CaCl2Has no obvious promotion effect on the growth of Tv-1511 under normal conditions. Under the salt stress, the growth of Trichoderma viride Tv-1511 is inhibited, and after the Trichoderma viride Tv-1511 is treated by 300mM NaCl for 72h, the colony radius of the Tv-1511 is 17.31mM shorter than that of a control group, and is reduced by 36.2%; adding 0.5mM CaCl2After that, the phenomenon that the growth of Tv-1511 hyphae is inhibited is obviously reduced, and after 72 hours of treatment, a T3 group (300mM NaCl +0.5mM CaCl)2) The colony radius was 1.67 times that of the T2 group (300mM NaCl) (FIG. 6). Therefore, exogenous calcium can significantly enhance the salt tolerance of trichoderma viride Tv-1511.
Example 4: saline-alkali soil trichoderma bio-organic fertilizer field demonstration
4.1. Test material
Test microorganisms: trichoderma viride Tv-1511.
Test work: wheat (Jimai 22) was selected by the institute of crops, institute of agricultural sciences, Shandong province. Corn (huanong 138), available from great seed science co.
Test Trichoderma organismsOrganic fertilizer: provided by Jinxing (sunshine) agriculture science and technology Limited, the water content is less than or equal to 30 percent, the organic matter is more than or equal to 45 percent, the total nitrogen is 4 percent, and the total phosphorus (P)2O5) 4% total potassium (K)2O) 2%, zinc sulfate (ZnSO)4) 0.05% and 1% of free Amino Acid (Amino Acid). Adding the trichoderma solid fermentation product according to 5% of the dry weight of the organic fertilizer to prepare the biological organic fertilizer.
Trichoderma solid fermentation: inoculating Trichoderma viride Tv-1511 at 6% inoculation amount in culture medium containing mushroom residue, and culturing at 30 deg.C for 20 days.
The method comprises the following steps of (1) testing the saline-alkali soil trichoderma bio-organic fertilizer: provided by Venus aureus (sunshine) agricultural science and technology Limited company, and a certain proportion of calcium superphosphate is added on the basis of the trichoderma type bio-organic fertilizer.
4.2. Design of experiments
The field demonstration test is carried out in the high and new technology industry demonstration area of yellow river delta agriculture in Shandong province. The content of organic matters in the soil is 0.61-1.01%, the content of the organic matters is not high, and the soil is high in potassium, low in nitrogen and low in phosphorus; the pH value of the soil is alkaline and is higher than 8.0; the salt content is 0.3-0.6% and belongs to moderately heavily salinized soil. The experimental design was as follows: using the fertilizer applied by farmers as a control group (CK); application of the trichoderma bio-organic fertilizer is treatment 1 (T1); on the basis of the trichoderma bio-organic fertilizer, the treatment 2(T2) is added with calcium superphosphate (the adding amount of corn is 19.95 kg/mu, and the adding amount of wheat is 28.50 kg/mu), each treatment is repeated for 3 times, the conventional management is carried out, and the yield is measured by weighing after the fertilizer is mature.
4.3. Results
The influence of the trichoderma bio-organic fertilizer on the thousand-grain weight and yield of wheat in saline-alkali soil is shown in table 1. The thousand grain weight of the wheat after the treatment of T1 (the application of the trichoderma type bio-organic fertilizer) and T2 (the addition of calcium superphosphate on the basis of the trichoderma type bio-organic fertilizer) is respectively 1.03 times and 1.04 times of that of CK, wherein the influence of the treatment of T2 on the thousand grain weight of the wheat reaches a remarkable level (P is less than 0.05).
Compared with CK (conventional fertilization) treatment, T1 (application of trichoderma bio-organic fertilizer) increases yield of 42.01kg per mu of wheat by 11.90%; the yield of each mu of wheat treated by T2 (added with calcium superphosphate on the basis of the trichoderma type bio-organic fertilizer) is increased by 66.91kg and 18.95 percent. Both treatments achieved significant levels of difference (P < 0.05). Therefore, the application of the trichoderma type bio-organic fertilizer and the addition of the calcium superphosphate on the basis of the trichoderma type bio-organic fertilizer can both obviously increase the yield of wheat, and the yield increasing effect is most obvious in a fertilizing mode of adding the calcium superphosphate on the basis of the trichoderma type bio-organic fertilizer.
TABLE 1 influence of Trichoderma bio-organic fertilizer in saline-alkali soil on thousand-grain weight and yield of wheat
Figure BDA0002825731430000111
The influence of the trichoderma bio-organic fertilizer on the hundred-grain weight and yield of the saline-alkali soil corns is shown in table 2. Compared with CK (conventional fertilization) treatment, the weight of the corn treated by T1 (trichoderma bio-organic fertilizer application) and T2 (trichoderma bio-organic fertilizer with calcium superphosphate added) is increased by 5.54% and 6.62%, respectively. Wherein the effect of the T2 treatment on the hundred grain weight of the corn reaches a significant level (P < 0.05).
Compared with CK (conventional fertilization) treatment, T1 (trichoderma bio-organic fertilizer application) increases the yield of corn acre by 61.94kg and by 15.48%; the yield of corn acre treated by T2 (added with calcium superphosphate on the basis of trichoderma type bio-organic fertilizer) is increased by 126.19kg and 31.54 percent. Both treatments achieved significant levels of difference compared to CK (P < 0.05), and the T2 treatment also achieved significant levels of difference compared to the T1 treatment (P < 0.05). Therefore, the application of the trichoderma type bio-organic fertilizer and the addition of the calcium superphosphate on the basis of the trichoderma type bio-organic fertilizer can both obviously increase the yield of the corn, and the yield increasing effect is most obvious in a fertilizing mode of adding the calcium superphosphate on the basis of the trichoderma type bio-organic fertilizer.
TABLE 2 influence of Trichoderma bioorganic fertilizer in saline-alkali soil on corn grain weight and yield
Figure BDA0002825731430000121
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or equivalents thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Although the present invention has been described with reference to the specific embodiments, it should be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.

Claims (10)

1. The application of exogenous calcium in improving the salt tolerance of Trichoderma viride (Trichoderma viride) Tv-1511 or a bio-organic fertilizer containing Trichoderma viride (Trichoderma viride) Tv-1511.
2. The use of claim 1, wherein the exogenous calcium is calcium chloride or calcium superphosphate;
preferably, the concentration of the exogenous calcium is controlled to be 0.1-1 mM, and more preferably 0.5 mM.
3. A bio-organic fertilizer, characterized in that it comprises at least:
(a) trichoderma viride (Trichoderma viride) Tv-1511 or a solid fermentation thereof;
(b) a source of calcium.
4. The bio-organic fertilizer according to claim 3, wherein the preparation method of the solid fermentation product comprises the following steps: inoculating Trichoderma viride Tv-1511 into culture medium containing mushroom residue, and culturing at 20-30 deg.C for 15-25 days.
5. Bio-organic fertilizer according to claim 3, wherein the calcium source is in particular a calcium salt, preferably calcium superphosphate or calcium chloride.
6. The bio-organic fertilizer according to claim 3, wherein the addition amount of the solid fermentation product is 1-10% of the dry weight of the bio-organic fertilizer, preferably 5%;
preferably, the bio-organic fertilizer comprises solid fermentation product of Trichoderma viride Tv-1511, calcium source and organic fertilizer.
7. Use of Trichoderma viride (Trichoderma viride) Tv-1511 and/or a bio-organic fertilizer according to any one of claims 3 to 6 in any one or more of:
1) the application in inhibiting pathogenic bacteria;
2) the application in the preparation of pathogenic bacteria inhibitor;
3) the application in inhibiting plant diseases;
4) application in preparing plant disease inhibitor.
8. The use according to claim 7, wherein the pathogenic bacteria are all or part of the following: fusarium oxysporum (Fusarium oxysporum), Fusarium proliferatum (Fusarium proliferatum), and Sclerotinium rolfsii (Sclerotinium rolfsii).
9. The use according to claim 7, characterized in that the diseases are all or some of the following diseases: blight, ear rot and southern blight.
10. Use of Trichoderma viride (Trichoderma viride) Tv-1511 and/or a bioorganic fertilizer according to any one of claims 3 to 6 for promoting crop yield increase;
preferably, the crops are corn and wheat;
preferably, in the application, the application environment is a saline-alkali environment.
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