CN109182179A - A kind of kitchen garbage degradation agent and preparation method thereof - Google Patents
A kind of kitchen garbage degradation agent and preparation method thereof Download PDFInfo
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- CN109182179A CN109182179A CN201811062876.3A CN201811062876A CN109182179A CN 109182179 A CN109182179 A CN 109182179A CN 201811062876 A CN201811062876 A CN 201811062876A CN 109182179 A CN109182179 A CN 109182179A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a kind of kitchen garbage degradation agents and preparation method thereof, belong to environmental protection technical field.Kitchen garbage degradation agent provided by the invention includes the bacillus subtilis SL-10 (Bacillus subtilis SL-10) for having good degradation effect to kitchen organic matter, the strain is preserved in China typical culture collection center at present, the deposit date is on June 28th, 2017, deposit number was CCTCC M2017387.The kitchen organic waste degradation agent preparation method is simple, and good degrading effect, application potential is big, can solve the problems, such as the problem of resource waste of environmental contaminants caused by discarded kitchen garbage and nutrition organic matter.
Description
Technical field
The present invention relates to field of environment engineering technology, it is specifically related to a kind of kitchen garbage degradation agent and preparation method thereof.
Background technique
Circular economy efficiently utilizes and is recycling as core with resource, is original with " minimizing, recycling, recycling "
It then, is stream with " resource-product-regenerated resources " mode of reaction type using low consumption, low emission, high efficiency as essential characteristic
Journey, emphasize decrement, recycle on the basis of realize resource circulation utilization, including substance circulation and energy circulation, by from source
Head reduces consumption, realizes recycle in the process, and discharge is reduced in end, reaches recycling economy development requirement.
Kitchen castoff refers generally to the easy organic matter being decomposed by the microorganisms such as leftovers, leftovers and dish leaf, pericarp, to form sediment
Based on the substances such as powder class, food fiber class, animal protein, fat, mostly come from the processes such as food processing, processing, transport with
And surplus meal topples over object, has moisture content high, grease, salt content are high, perishable, fermentation, it is smelly the features such as, occupy a large amount of soil
Ground seriously pollutes atmosphere, water body, easily causes biologic contamination.
Degradation treatment is carried out to kitchen waste using microbial method, bio-organic fertilizer can be obtained, avoid the wasting of resources
With the pollution to environment.Common processing method is divided into aerobic method and two kinds of anaerobic process at present.Wherein, aerobic method is by aerobic micro-
Biodegradable organic waste, speed is fast, compost temperature is high, is divided into fever, heating, cooling and decomposed fertilizer conservation four-stage, micro-
Biological bacteria concomitant fermentations process and change.Anaerobic composting method refers to anaerobe under anaerobic state by organic solid matter
It liquefied, make organic substance conversion and stabilized process after acid fermentation, alkaline fermentation.
As the Classification Management of family's organic matter class rubbish is promoted, kitchen garbage is more and more carried out in family at present
Classification saves and processing, and the equipment of kitchen garbage disposal and the demand of kitchen organic matter degradation agent increase year by year suitable for family
It is more, microbial bacterial agent is added into carrier using biological reinforcing technology and strengthen exploitation kitchen organic matter degradation agent with good
Prospect and effect.
Summary of the invention
Based on the above background, the present invention develops the kitchen organic matter drop under aerobic condition to realize resource circulation utilization
Solve agent.
To achieve the object of the present invention, the present invention provides a kind of kitchen garbage degradation agent, which includes to have to kitchen
Machine matter has the bacillus subtilis SL-10 (Bacillus subtilis SL-10) of good degradation effect, which protects at present
It is hidden in China typical culture collection center, the deposit date is on June 28th, 2017, deposit number was CCTCC M2017387.
The present invention also provides a kind of preparation methods of kitchen garbage degradation agent, this method comprises: Spawn incubation obtains
Seed liquor is inoculated with to obtain fermentation liquid, carrier sterilizing, carrier and fermentation liquid mixing, obtains kitchen garbage degradation agent.
The Spawn incubation method can be with are as follows: after the slant culture of bacillus subtilis SL-10 is seeded to sterilizing
In seed culture medium, is cultivated 8-24 hours at 20-40 DEG C of temperature, 120-220rpm revolving speed, obtain seed liquor.
Further, the seed culture medium pH value is 6-9, and every liter of seed culture medium may include peptone 5-15g, ox
Meat extract 3-8g, sodium chloride 2-4g.
The inoculation, which can be, is inoculated in seed liquor in fermentation medium with the ratio of 2-10%, obtains fermentation liquid.
Preferably, the fermentation medium sterilizing is followed by kind of a seed liquor, at 20-40 DEG C of temperature, 120-220rpm revolving speed
Cultivate 2-7d.
Further, the fermentation medium pH value can be 6-9, and every liter may include peptone 5-15g, beef extract 3-
8g, sucrose 10-20g, sodium chloride 2-4g, potassium dihydrogen phosphate 2-6g, dipotassium hydrogen phosphate 1-5g, magnesium sulfate 1-3g.
The carrier may include one or more of wheat bran, rice bran, sawdust, husk, active carbon, rice chaff and Chinese medicine slag
Combination.
Preferably, carrier includes wheat bran, sawdust, husk and active carbon；It is further preferred that the mass ratio of each ingredient is bran
Skin 10-20%, sawdust 40-60%, active carbon 10-15%, husk 5-40%.
The seed culture medium sterilizing, fermentation medium sterilizing and carrier sterilizing can be and sterilize in 115-125 DEG C of temperature
15-30 minutes, such as sterilize after twenty minutes in 121 DEG C of temperature, cooling is spare.
Compared with prior art, advantages of the present invention is as follows:
(1) bacillus subtilis SL-10 of the invention has good growth ability, is fixed in cheap organic matter and carries
After on body, there is good survival ability, can rapidly and efficiently degrade kitchen garbage organic matter.
(2) degradation effect for the kitchen garbage degradation agent that the present invention obtains is significant, and preparation method is simple and quick, is suitable for exhausted
Most of kitchen garbage mechanism of degradation are suitable for average family use.
(3) kitchen garbage degradation agent realizes well degrading and recycle for kitchen garbage after, solves family
The processing problem and problem of resource waste of kitchen organic waste, environmental protection and economy are convenience-for-people to benefit the nation.
Detailed description of the invention
Fig. 1 is picture of the kitchen garbage before kitchen garbage degradation agent processing of the invention；
Fig. 2 is the picture after the kitchen garbage of Fig. 1 is handled 48 hours with kitchen garbage degradation agent of the invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.Additional aspect and advantage of the invention will be set forth in part in the description, part
It will become apparent from the description below, or practice through the invention is recognized.It is only used to solve it should be appreciated that being described below
The present invention is released, is not intended to limit the present invention.
Term "comprising" used herein, " comprising ", " having ", " containing " or its any other deformation, it is intended that covering
Non-exclusionism includes.For example, composition, step, method, product or device comprising listed elements are not necessarily limited to those and want
Element, but may include not expressly listed other elements or such composition, step, method, product or device it is intrinsic
Element.
Conjunction " Consists of " excludes any element that do not point out, step or component.If in claim, this
Phrase will make claim closed, so that it is not included the material in addition to the material of those descriptions, but relative normal
Except rule impurity.When being rather than immediately following after theme in the clause that phrase " Consists of " appears in claim main body,
It is only limited to element described in the clause；Other elements are not excluded except the claim as a whole.
Equivalent, concentration or other values or parameter are excellent with range, preferred scope or a series of upper limit preferred values and lower limit
When the Range Representation that choosing value limits, this should be understood as specifically disclosing by any range limit or preferred value and any range
Any pairing of lower limit or preferred value is formed by all ranges, regardless of whether the range separately discloses.For example, when open
When range " 1 to 5 ", described range should be interpreted as including range " 1 to 4 ", " 1 to 3 ", " 1 to 2 ", " 1 to 2 and 4 to
5 ", " 1 to 3 and 5 " etc..When numberical range is described herein, unless otherwise stated, otherwise the range is intended to include its end
Value and all integers and score in the range.
In addition, technical characteristic involved in each embodiment of the present invention as long as they do not conflict with each other can
To be combined with each other.
Embodiment 1
1. a kind of kitchen garbage degradation agent and preparation method thereof, process flow is as follows:
(1) prepare seed culture medium: every liter of seed culture medium contains peptone 10g, beef extract 5g, sodium chloride 3g, pH value
6.6, sterilizing at a temperature of 121 DEG C, it is stand-by to be cooled to room temperature after twenty minutes；
(2) 1 ring of the bacillus subtilis SL-10 on inclined-plane is inoculated in seed culture medium, at a temperature of 28 DEG C
20 hours are cultivated under 200rpm revolving speed to logarithmic growth phase, obtain seed liquor；
(3) 100L fermentation medium is prepared, every liter of fermentation medium contains peptone 10g, beef extract 5g, sucrose 15g,
Sodium chloride 3g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 1g, magnesium sulfate 1g, pH value 6.8, culture medium is through 121 DEG C of temperature sterilizings 20
Cooling is spare after minute；
(4) seed liquor that step (2) obtain is inoculated in fermentation medium with 5% ratio, in 30 DEG C of temperature,
It is cultivated 3 days under 150rpm revolving speed, it is spare to obtain fermentation of bacillus liquid, at this point, counting to get clump count 1*1010CFU/mL；
(5) 500 kilograms of carrier are prepared, wherein 100 kilograms of wheat bran, 300 kilograms of sawdust, 75 kilograms of active carbon, husk 25 are public
Jin, sterilizes 20 minutes at a temperature of 121 DEG C after being sufficiently mixed, spare after cooling；
(6) fermentation liquid that step (4) obtains is added in carrier, kitchen organic matter degradation is packaged to be after being sufficiently mixed
Agent.
2. 1 kilogram of degradation agent is added in kitchen garbage mechanism of degradation, 0.4 kilogram of remaining food is added and carries out at degradation
Reason, effect are as shown in table 1: with the extension of time, the trend risen is presented in degradation rate, calculating degradation with the practical weight that mitigates
As a result rate reaches 93.2% in 48h degradation rate, degradation effect is good.
1 embodiment 1 of table degradation situation table
| Time (h) | Appearance | Degradation rate (%) |
| 0 | Kitchen organic matter is added | 0 |
| 6 | Organic matter mixing is abundant | 10.1 |
| 12 | Bulky grain has part reduction | 24.5 |
| 18 | Vegetables decompose and finish | 30.8 |
| 24 | Rice constituents reduce obvious | 50.4 |
| 36 | Kitchen organic matter significantly reduces | 85.6 |
| 48 | Basic degradation is complete | 93.2 |
Embodiment 2
1. a kind of kitchen garbage degradation agent and preparation method thereof, process flow is as follows:
(1) prepare seed culture medium: every liter of seed culture medium contains peptone 12g, beef extract 6g, sodium chloride 4g, pH value
7, and sterilize and be cooled to room temperature after twenty minutes for use at a temperature of 121 DEG C；
(2) 1 ring of the bacillus subtilis SL-10 on inclined-plane is inoculated in seed culture medium, at a temperature of 35 DEG C
16 hours are cultivated under 200rpm revolving speed to logarithmic growth phase, obtain seed liquor；
(3) 70L fermentation medium is prepared, every liter of fermentation medium contains peptone 10g, beef extract 5g, sucrose 20g, chlorine
Change sodium 3g, potassium dihydrogen phosphate 2g, dipotassium hydrogen phosphate 2g, magnesium sulfate 2g, pH value 7.0, culture medium sterilizes 20 minutes through 121 DEG C of temperature
After cool down it is spare；
(4) seed liquor that step (2) obtain is inoculated in fermentation medium with 10% ratio, in 30 DEG C of temperature,
3d is cultivated under 200rpm revolving speed, it is spare to obtain fermentation of bacillus liquid, at this point, counting to get clump count 1.5*1010CFU/mL；
(5) 400 kilograms of carrier are prepared, wherein 40 kilograms of wheat bran of weighing, 160 kilograms of sawdust, 40 kilograms of active carbon, husk
It 160 kilograms, sterilizes 20 minutes at a temperature of 121 DEG C after being sufficiently mixed, it is spare after cooling；
(6) fermentation liquid that step (4) obtains is added in carrier, kitchen organic matter degradation is packaged to be after being sufficiently mixed
Agent.
2. 2 kilograms of degradation agents is added in kitchen garbage mechanism of degradation, 1 kilogram of remaining food is added and carries out degradation treatment,
Effect is as shown in table 2: with the extension of time, the trend risen is presented in degradation rate, calculating degradation rate, knot with the practical weight that mitigates
Fruit reaches 94.8% in 48h degradation rate, and degradation effect is good.
2 embodiment 2 of table degradation situation table
| Time (h) | Appearance | Degradation rate (%) |
| 0 | Kitchen organic matter is added | 0 |
| 6 | Organic matter mixing is abundant | 12.2 |
| 12 | Bulky grain has part reduction | 26.8 |
| 18 | Vegetables decompose and finish | 42.2 |
| 24 | Rice constituents reduce obvious | 56.8 |
| 36 | Kitchen organic matter significantly reduces | 82.6 |
| 48 | Basic degradation is complete | 94.8 |
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all include
Within protection scope of the present invention.
Claims (10)
1. a kind of kitchen garbage degradation agent, which is characterized in that the degradation agent includes to have good degradation effect to kitchen organic matter
Bacillus subtilis SL-10 (Bacillus subtilis SL-10), the strain be preserved at present Chinese Typical Representative culture guarantor
Hiding center, the deposit date is on June 28th, 2017, deposit number was CCTCC M2017387.
2. a kind of preparation method of kitchen garbage degradation agent described in claim 1, which is characterized in that this method comprises: strain
Seed liquor is cultivated to obtain, fermentation liquid, carrier sterilizing, carrier and fermentation liquid mixing is inoculated with to obtain, obtains kitchen garbage degradation agent.
3. the preparation method of kitchen garbage degradation agent according to claim 2, which is characterized in that the Spawn incubation method
Are as follows: the slant culture of bacillus subtilis SL-10 is seeded in the seed culture medium after sterilizing, in 20-40 DEG C of temperature,
It is cultivated 8-24 hours under 120-220rpm revolving speed, obtains seed liquor.
4. the preparation method of kitchen garbage degradation agent according to claim 3, which is characterized in that the seed culture medium pH
Value is 6-9, and every liter of seed culture medium includes peptone 5-15g, beef extract 3-8g, sodium chloride 2-4g.
5. the preparation method of kitchen garbage degradation agent according to claim 2, which is characterized in that the inoculation is by seed
Liquid is inoculated in fermentation medium with the ratio of 2-10%, obtains fermentation liquid.
6. the preparation method of kitchen garbage degradation agent according to claim 5, which is characterized in that the fermentation medium goes out
Bacterium is followed by kind of a seed liquor, cultivates 2-7d at 20-40 DEG C of temperature, 120-220rpm revolving speed.
7. the preparation method of kitchen garbage degradation agent according to claim 5, which is characterized in that the fermentation medium pH
Value is 6-9, and every liter includes peptone 5-15g, beef extract 3-8g, sucrose 10-20g, sodium chloride 2-4g, potassium dihydrogen phosphate 2-6g,
Dipotassium hydrogen phosphate 1-5g, magnesium sulfate 1-3g.
8. the preparation method of kitchen garbage degradation agent according to claim 2, which is characterized in that the carrier includes bran
One of skin, rice bran, sawdust, husk, active carbon and Chinese medicine slag are a variety of.
9. the preparation method of kitchen garbage degradation agent according to claim 8, which is characterized in that the carrier includes bran
Skin, sawdust, husk, active carbon, it is preferred that the mass ratio of each ingredient is wheat bran 10-20%, sawdust 40-60%, active carbon 10-
15%, husk 5-40%.
10. according to the preparation method of kitchen garbage degradation agent described in claim 2-3 or 6, which is characterized in that the seed training
Supporting base sterilizing, fermentation medium sterilizing and carrier sterilizing is sterilized 15-30 minutes in 115-125 DEG C of temperature, such as in 121 DEG C of temperature
After twenty minutes, cooling is spare for degree sterilizing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811062876.3A CN109182179A (en) | 2018-09-12 | 2018-09-12 | A kind of kitchen garbage degradation agent and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811062876.3A CN109182179A (en) | 2018-09-12 | 2018-09-12 | A kind of kitchen garbage degradation agent and preparation method thereof |
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| CN201811062876.3A Pending CN109182179A (en) | 2018-09-12 | 2018-09-12 | A kind of kitchen garbage degradation agent and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468119A (en) * | 2019-07-01 | 2019-11-19 | 福建美天环保科技有限公司 | A kind of garbage disposal microbial activity medium carrier |
| CN110894477A (en) * | 2019-09-18 | 2020-03-20 | 浙江工业大学 | Compound microbial inoculum for degrading kitchen waste, application and kitchen waste degradation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107739726A (en) * | 2017-11-15 | 2018-02-27 | 浙江科技学院 | A kind of bacillus subtilis and its application |
-
2018
- 2018-09-12 CN CN201811062876.3A patent/CN109182179A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107739726A (en) * | 2017-11-15 | 2018-02-27 | 浙江科技学院 | A kind of bacillus subtilis and its application |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468119A (en) * | 2019-07-01 | 2019-11-19 | 福建美天环保科技有限公司 | A kind of garbage disposal microbial activity medium carrier |
| CN110894477A (en) * | 2019-09-18 | 2020-03-20 | 浙江工业大学 | Compound microbial inoculum for degrading kitchen waste, application and kitchen waste degradation method |
| CN110894477B (en) * | 2019-09-18 | 2021-03-23 | 浙江工业大学 | Compound microbial inoculum for degrading kitchen waste, application and kitchen waste degradation method |
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Application publication date: 20190111 |