CN109169715A - A kind of complex biological bactericide composition containing Paenibacillus polymyxa and bacillus subtilis - Google Patents
A kind of complex biological bactericide composition containing Paenibacillus polymyxa and bacillus subtilis Download PDFInfo
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- CN109169715A CN109169715A CN201811059166.5A CN201811059166A CN109169715A CN 109169715 A CN109169715 A CN 109169715A CN 201811059166 A CN201811059166 A CN 201811059166A CN 109169715 A CN109169715 A CN 109169715A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
The invention discloses a kind of complex biological bactericide composition containing Paenibacillus polymyxa and bacillus subtilis, belong to biological pesticide technical field, its effective ingredient is Paenibacillus polymyxa (Paenibacillus polymyza) and bacillus subtilis (Bacillus subtilis), wherein the content of Paenibacillus polymyxa is hundred million CFU/ g of formulation of 0.1-100, and the content of bacillus subtilis is hundred million CFU/ g of formulation of 1-10000.Paenibacillus polymyxa and bacillus subtilis mutually cooperate with, and can play a role simultaneously from different targets, can greatly improve drug effect, reduce drug cost, delay the resistance of disease, comprehensive protection is provided for crop.By adding appropriate auxiliary material, wettable powder, water dispersible granules or microcapsulated granule can be made in Paenibacillus polymyxa and bacillus subtilis, for preventing and treating the multiple diseases such as bacterial wilt, bacterial angular leaf spot, powdery mildew, wilt disease, downy mildew, bacterial black rot, bacterial stripe, bacterial leaf spot, banded sclerotial blight, rice blast, ring rot, soft rot, canker, balck shank.
Description
Technical field
The present invention relates to biological pesticide technical fields, and in particular to one kind contains Paenibacillus polymyxa and bacillus subtilis
Complex biological bactericide composition, Paenibacillus polymyxa and bacillus subtilis compound preparation is made in certain proportion
Afterwards, can be used for preventing and treating bacterial wilt, bacterial angular leaf spot, powdery mildew, wilt disease, downy mildew, bacterial black rot, bacterial stripe
The multiple diseases such as disease, bacterial leaf spot, banded sclerotial blight, rice blast, ring rot, soft rot, canker, balck shank.
Background technique
China uses a large amount of chemical pesticide preparations every year, easily causes excessive pesticide residues, biological concentration, not only pollution life
State environment, has an effect on the health of the mankind, and biological pesticide Small side effects, harmless to humans, is a kind of green with bright prospects
Pesticide has become the trend of global pesticide development.
Bacillus is that a kind of more microorganism is studied in current biological pesticide, it has broad spectrum antibiotic activity and extremely strong
Anti-adversity ability, vegetative cell can fungal pathogen mycelium surface multiply colonize, effectively inhibition pathogen growth, therefore,
In the generation of control disease, promote plant strain growth, improves crop yield etc. and show wide application prospect.
Paenibacillus polymyxa (Paenibacillus polymyza) is a kind of biocontrol bacteria, to the bacillary angle of crop
The plurality of plant diseases such as pinta, bacterial wilt, soft rot, canker, anthracnose, wilt disease, verticillium wilt, root rot all have centainly
Preventive and therapeutic effect.
Bacillus subtilis (Bacillus subtilis) is a kind of mesophilic, aerobic, sporiferous bacillus, life
Feature multiplicity is managed, it is widely distributed, easily it is separately cultured.The bacterium is widespread in nature, nontoxic to people and animals, does not pollute
Environment can generate a variety of antibiotic and enzyme, have broad spectrum antibiotic activity and extremely strong anti-adversity ability.Bacillus subtilis not only may be used
It to be widely present in the external environments such as soil, plant rhizosphere, body surface, and is common endogenetic bacteria in plant, especially
Root, stem in plant.The bacterium shows on the crops such as rice, soybean, cotton, wheat, capsicum, tomato, corn at present
Good disease-controlling effect is shown.Bacillus subtilis pass through successfully colonize to plant rhizosphere, body surface or in vivo, with pathogen
Living space around competitive plant secretes antibacterial material to inhibit growth of pathogenic bacteria, while plant defense system being induced to resist
Pathogen invasion, to achieve the purpose that biological and ecological methods to prevent plant disease, pests, and erosion.Its main controlling object is plant disease, such as rice caused by filamentous fungi
Banded sclerotial blight (Stagonospora curtisii), wheat sharp eyespot (Rhizoctonia cerealis), leaf muld of tomato
(Cladosporium fulvum), beans root rot (Fusarium graminerarum), Apple Mould Core (Alternaria
Alternata), cotton seedling blight (Rhizoctonia solani), cotton wilt (Fusarium oxysporum
F.sp.vasinfectum) etc..
Summary of the invention
The present invention is a kind of complex biological bactericide composition containing Paenibacillus polymyxa and bacillus subtilis, is belonged to
Biological pesticide technical field.
The Paenibacillus polymyxa and bacillus subtilis content ratio is 0.1-100 hundred million CFU/ grams: 1-10000 hundred million
CFU/ grams, remaining for allow in pesticide using with acceptable auxiliary element.
It can be wettable powder, water-dispersible grain according to the dosage form of aforementioned proportion biological bactericide composition of the invention
Any in agent, microcapsulated granule, need to only arrange in pairs or groups corresponding auxiliary element.
Complex biological bactericide composition of the invention has mutually synergistic effect, can send out brightness from different targets simultaneously
Effect greatly improves drug effect, reduces drug cost, delays the resistance of disease, comprehensive protection is provided for crop.
The microbicide compositions can be used for preventing and kill off the bacterial wilt of crops, bacterial angular leaf spot, powdery mildew, wither
Disease, downy mildew, bacterial black rot, bacterial stripe, bacterial leaf spot, banded sclerotial blight, rice blast, ring rot, soft rot, ulcer
Disease, balck shank etc..
Compared with existing biological pesticide, the present invention has the advantages that
1, Paenibacillus polymyxa and bacillus subtilis compounding have significant synergistic function, compared with exclusive use
A kind of strain significantly improves the control efficiency to crop disease.
2, the mechanism of action of Paenibacillus polymyxa and bacillus subtilis is different from chemical pesticide, can effectively delay disease
The generation of drug resistance increases the service life of pesticide.
3, Paenibacillus polymyxa and bacillus subtilis easily obtain in the Nature, and the market price is relatively low, will
Biological pesticide is made in it, can be effectively reduced drug cost.
Specific embodiment
It is further described below with reference to the technical solution that embodiment is related to invention, to facilitate reason of the invention
Solution, but not as the limitation to technical solution, in addition to specifically mentioned, component ratio is weight percentage.
Embodiment 1
200000000 CFU/ grams of bacillus subtilis granules of CFU/ grams of Paenibacillus polymyxa 500,000,000
Formula: weighing Paenibacillus polymyxa (female medicine: 5,000,000,000 CUF/ grams) 4% according to quantity, bacillus subtilis (female medicine:
1000000000000 CUF/ grams) 0.05%, sodium lignin sulfonate 3.2% (dispersing agent), lauryl sodium sulfate 2.5% (wetting agent) is gathered
Vinyl alcohol 2.5% (binder), white carbon black 10% (filler), diatomite (filler) complement to 100%.By above each ingredient by matching
The ratio of side is sufficiently mixed, and is crushed, is stirred again after wetting with water, then be granulated with screw extruder pelletizer, after dry
Sieving is to get 200,000,000 CFU/ grams of bacillus subtilis granules of CFU/ grams of Paenibacillus polymyxa 500,000,000.
Field trial 1:
Experimental pharmacy is 200,000,000 CFU/ grams of bacillus subtilis granules of CFU/ grams of Paenibacillus polymyxa 500,000,000, by implementation
Example 1 is prepared, and comparison medicament is 200,000,000 CFU/ grams of water dispersible powder of Paemobacillus polymyxa, 500,000,000 CFU/ grams of bacillus subtilis
Bacterium；Controlling object is cucumber downy mildew；It is as follows for the control rate data of downy mildew:
From field trial 1 as can be seen that in the test of prevention and treatment cucumber downy mildew, 200,000,000 CFU/ grams of mostly viscous class gemma bars
The effect of the CFU/ grams of bacillus subtilis granule of bacterium 500,000,000 is better than 200,000,000 CFU/ grams of water dispersible powder of Paemobacillus polymyxa and 5
Hundred million CFU/ grams of bacillus subtilis wettable powders, and synergy is obvious.
Embodiment 2
500000000 CFU/ grams of bacillus subtilis granules of CFU/ grams of Paenibacillus polymyxa 1,000,000,000
Formula: weighing Paenibacillus polymyxa (female medicine: 5,000,000,000 CUF/ grams) 10% according to quantity, bacillus subtilis (female medicine:
1000000000000 CUF/ grams) 0.1%, naphthalenesulfonate formaldehyde condensation compound 3.5% (dispersing agent), (wetting of lauryl sodium sulfate 3.5%
Agent), polyvinyl alcohol 3.0% (binder), attapulgite 10% (filler), diatomite (filler) complements to 100%.It will be above each
Ingredient is mixed according to the formula uniformly, through air-flow crushing at pulvis, a certain amount of water mixing extruding is added and makes material, dry screening
After obtain 500,000,000 CFU/ grams of bacillus subtilis granule granules of CFU/ grams of Paenibacillus polymyxa 1,000,000,000.
Field trial 2:
Experimental pharmacy is 500,000,000 CFU/ grams of bacillus subtilis granules of CFU/ grams of Paenibacillus polymyxa 1,000,000,000, by real
It applies example 2 to be prepared, comparison medicament is 500,000,000 CFU/ grams of water dispersible powder of Paemobacillus polymyxa, 1,000,000,000 CFU/ grams of withered grass gemma
Bacillus；Controlling object is cucumber downy mildew；It is as follows for the control rate data of downy mildew:
From field trial 2 as can be seen that in the test of prevention and treatment cucumber downy mildew, 500,000,000 CFU/ grams of mostly viscous class gemma bars
The effect of the CFU/ grams of bacillus subtilis granule of bacterium 1,000,000,000 better than 500,000,000 CFU/ grams of water dispersible powder of Paemobacillus polymyxa and
1000000000 CFU/ grams of bacillus subtilis wettable powders, and synergy is obvious.
Embodiment 3
1000000000 CFU/ grams of bacillus subtilis wettable powders of CFU/ grams of Paenibacillus polymyxa 10,000,000,000
Formula: weighing Paenibacillus polymyxa (female medicine: 5,000,000,000 CUF/ grams) 20% according to quantity, bacillus subtilis (female medicine:
1000000000000 CUF/ grams) 1%, polycarboxylate 5.0% (dispersing agent), lauryl sodium sulfate 4.5% (wetting agent), white carbon black
10% (filler), kaolin (filler) complement to 100%.Above each ingredient is sufficiently mixed in the ratio of formula uniformly, through gas
It flows pulverizer to crush, can be prepared by wettable containing 1,000,000,000 CFU/ grams of bacillus subtilises of CFU/ grams of Paenibacillus polymyxa 10,000,000,000
Property pulvis.
Field trial 3:
Experimental pharmacy is 1,000,000,000 CFU/ grams of bacillus subtilis wettable powders of CFU/ grams of Paenibacillus polymyxa 10,000,000,000
Agent is prepared by embodiment 3, and comparison medicament is 1,000,000,000 CFU/ grams of water dispersible powder of Paemobacillus polymyxa, and 10,000,000,000 CFU/ grams
Bacillus subtilis；Controlling object is cucumber downy mildew；It is as follows for the control rate data of downy mildew:
From field trial 3 as can be seen that in the test of prevention and treatment cucumber downy mildew, 1,000,000,000 CFU/ grams of mostly viscous class gemma bars
The effect of the CFU/ grams of bacillus subtilis wettable powder of bacterium 10,000,000,000 is better than 1,000,000,000 CFU/ grams of Paenibacillus polymyxa wettables
Pulvis and 10,000,000,000 CFU/ grams of bacillus subtilis wettable powders, and synergy is obvious.
The field test results of above-described embodiment show that the compound product of Paenibacillus polymyxa and bacillus subtilis is imitated
Fruit is substantially better than single-dose product, and popularization and application have huge economic results in society.
Claims (6)
1. a kind of complex biological bactericide composition, which is characterized in that its effective ingredient is Paenibacillus polymyxa
(Paenibacillus polymyza) and bacillus subtilis (Bacillus subtilis).
2. microbicide compositions according to claim 1, which is characterized in that the Paenibacillus polymyxa and withered grass bud
The living bacteria count amount ratio of spore bacillus is CFU/ grams of 0.1-100 hundred million CFU/ grams: 1-10000 hundred million.
3. microbicide compositions described in any one of -2 claims according to claim 1, which is characterized in that the fungicide
Composition is aided with Pesticide formulation auxiliary element and is processed into any one dosage form for being suitble to agriculturally use.
4. microbicide compositions according to claim 3, which is characterized in that dosage form be wettable powder, water dispersible granules,
It is any in microcapsulated granule.
5. the purposes that microbicide compositions described in any one of claim 1-4 claim are used to prevent and kill off corps diseases.
6. purposes according to claim 5, which is characterized in that the disease is bacterial wilt, bacterial angular leaf spot, white powder
Disease, wilt disease, downy mildew, bacterial black rot, bacterial stripe, bacterial leaf spot, banded sclerotial blight, rice blast, ring rot, soft corruption
Disease, canker, balck shank etc..
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811059166.5A CN109169715A (en) | 2018-09-05 | 2018-09-05 | A kind of complex biological bactericide composition containing Paenibacillus polymyxa and bacillus subtilis |
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| CN201811059166.5A CN109169715A (en) | 2018-09-05 | 2018-09-05 | A kind of complex biological bactericide composition containing Paenibacillus polymyxa and bacillus subtilis |
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Cited By (4)
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| CN109879678A (en) * | 2019-03-20 | 2019-06-14 | 山东省果树研究所 | A kind of composite microbic bacterial fertilizer and its preparation method and application |
| CN112400901A (en) * | 2020-11-30 | 2021-02-26 | 安徽丰乐农化有限责任公司 | Bactericidal composition and application thereof |
| CN112674116A (en) * | 2020-12-23 | 2021-04-20 | 宝鸡市星星协力生物有限公司 | Compound microbial agent for preventing and treating scab of crops and preparation and use methods thereof |
| CN113287636A (en) * | 2021-05-17 | 2021-08-24 | 新疆库庚尔奇农业科技有限公司 | Novel plant seed dressing agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109879678A (en) * | 2019-03-20 | 2019-06-14 | 山东省果树研究所 | A kind of composite microbic bacterial fertilizer and its preparation method and application |
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| CN112674116A (en) * | 2020-12-23 | 2021-04-20 | 宝鸡市星星协力生物有限公司 | Compound microbial agent for preventing and treating scab of crops and preparation and use methods thereof |
| CN113287636A (en) * | 2021-05-17 | 2021-08-24 | 新疆库庚尔奇农业科技有限公司 | Novel plant seed dressing agent |
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Application publication date: 20190111 |