CN101836638B - Bactericide for preventing and curing wheat scab - Google Patents
Bactericide for preventing and curing wheat scab Download PDFInfo
- Publication number
- CN101836638B CN101836638B CN201010202322.6A CN201010202322A CN101836638B CN 101836638 B CN101836638 B CN 101836638B CN 201010202322 A CN201010202322 A CN 201010202322A CN 101836638 B CN101836638 B CN 101836638B
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- China
- Prior art keywords
- bactericide
- tebuconazole
- triazolone
- wheat
- agent
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- 241000209140 Triticum Species 0.000 title claims abstract description 33
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 33
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 25
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 24
- 206010039509 Scab Diseases 0.000 title claims abstract description 18
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000005839 Tebuconazole Substances 0.000 claims abstract description 34
- FFSJPOPLSWBGQY-UHFFFAOYSA-N triazol-4-one Chemical compound O=C1C=NN=N1 FFSJPOPLSWBGQY-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000004480 active ingredient Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 21
- 201000010099 disease Diseases 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 241000221785 Erysiphales Species 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 206010059866 Drug resistance Diseases 0.000 abstract 1
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 13
- 231100000419 toxicity Toxicity 0.000 description 7
- 230000001988 toxicity Effects 0.000 description 7
- 241000223195 Fusarium graminearum Species 0.000 description 6
- 101710146773 Lanosterol 14-alpha demethylase Proteins 0.000 description 6
- 238000003209 gene knockout Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 230000002195 synergetic effect Effects 0.000 description 6
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 5
- 102100021695 Lanosterol 14-alpha demethylase Human genes 0.000 description 5
- 239000006013 carbendazim Substances 0.000 description 5
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 5
- 101150033031 CYP51A gene Proteins 0.000 description 4
- 101150087345 CYP51C gene Proteins 0.000 description 4
- 206010034133 Pathogen resistance Diseases 0.000 description 4
- 101150065362 cyp51B gene Proteins 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 150000003852 triazoles Chemical class 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000003449 preventive effect Effects 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- 101100061218 Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) CYP51C gene Proteins 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 101150065528 CYP51 gene Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001507673 Penicillium digitatum Species 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 239000005842 Thiophanate-methyl Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000017858 demethylation Effects 0.000 description 1
- 238000010520 demethylation reaction Methods 0.000 description 1
- 229930002954 deoxynivalenol Natural products 0.000 description 1
- -1 deoxynivalenol enol Chemical class 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 150000002561 ketenes Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bactericide for preventing and curing wheat scab, which comprises triazolone and tebuconazole as active components. The bactericide of the invention has stable chemical properties; in the mixing process, triazolone and tebuconazole are mutually soluble and do not generate chemical reaction; the bactericide has wide range of application, obvious synergy and safe use; and the bactericide has good antibacterial activity to gibberellic groups of wheat of different types in the field at present, and the antibacterial activity of the bactericide is obviously higher than the antibacterial activity of a single triazolone agent or a single tebuconazole agent, i.e. the bactericide has compound synergy of the triazolone agent and the tebuconazole agent. The compound agent also has good effect on preventing other important diseases such as wheat powdery mildew and the like in wheat production, has broad-spectrum sterilizing effect, can delay generation of drug resistance of pathogenic bacteria to single agents in the formula, and has significance for resistance management.
Description
Technical field
The present invention relates to Plant Protection field, especially relate to a kind of bactericide of preventing and treating wheat scab.
Background technology
Head blight is a kind of important disease on wheat crops, especially with wheat, is injured the heaviest.It is Gibberella zeae (Schw.) Petch that pathogen has condition, without condition, is Fusarium graminearum SchW., is mainly distributed in Winter Wheat Area, the middle and lower reach of Yangtze River and Northeasten Spring Wheat Area of China, and Upper Yangtze River and Winter Wheat Area, south China also often occur.After this disease occurs, the general underproduction 10～20%, reaches 80～90% when serious.In addition, contain the multiple toxin harmful to people, animal such as deoxynivalenol enol, Gibberella zeae ketenes in sick wheat, serious harm food security, carrys out serious potential threat to food manufacturing belt.
At present owing to producing the upper effective wheat-resistance to scab kind that lacks, control for wheat scab, in production, mainly rely on for many years benzimidazole germicide (as carbendazim, thiophanate-methyl), due to long-term single drug, field carbendazim high resistance bacterial strain has been found at present a lot of areas, pesticide resistance problem is more and more serious, and being badly in need of finding some has the replacement medicament of good fungistatic effect to head blight.Triazole bactericidal agent (as triazolone, Tebuconazole), belong to sterol demethylation inhibitor (DMI), this series bactericidal agent is a class of the most species of success commercialization in current bactericide market, a lot of field trials shows that triazole type Tebuconazole bactericide has good control efficiency for sickle-like bacteria, will be expected to become the important alternative medicament of preventing and treating wheat scab.
Summary of the invention
The invention provides a kind of bactericide of preventing and treating wheat scab, solved that pathogen easily develops immunity to drugs to existing bactericide or the not strong problem of existing bactericide single dose drug effect.
Prevent and treat a bactericide for wheat scab, active ingredient is triazolone and Tebuconazole, and above-mentioned active ingredient can and be filled composition with conventional auxiliary agent and is together mixed with the conventional formulations of pesticide.
The weight ratio of triazolone and Tebuconazole is preferably 1: 10～and 10: 1, be preferably 1: 1.Described triazolone and the weight concentration of Tebuconazole are respectively 9%～30% and 12.5%～80%, and general single dose all reaches above-mentioned concentration range.
The mechanism that above-mentioned bactericide drug effect strengthens is as follows:
14 α-demethylase (CYP51) is the action target spot of triazole bactericidal agent, in most organisms, only there is a CYP51, but from fusarium graminearum genome, but search the homologue of 3 CYP51, be respectively CYP51A, CYP51B and CYP51C, compare with the CYP51 having reported in other pathogen (wheat white powder, Penicillium digitatum, aspergillus flavus etc.), find all there is higher autoploidy (50～60%), and the result of reverse transcription-PCR shows that above-mentioned three CYP51 genes all can normal expression.
Utilize gene Knockout respectively by these three CYP51 gene knockouts, the transformant that knocks out of tri-genes of CYP51A, CYP51B and CYP51C is compared with wild-type parent, and triazolone and Tebuconazole are shown to different sensitivity differences.CYP51A gene knockout mutant significantly increases the susceptibility of triazolone, and CYP51B, CYP51C gene knockout mutant to the susceptibility of triazolone without significant change; CYP51A, CYP51C gene knockout mutant significantly increase the susceptibility of Tebuconazole, and CYP51B gene knockout mutant to the susceptibility of Tebuconazole without significant change.Illustrate that triazolone and Tebuconazole act on different CYP51, thereby according to this mechanism, by triazolone with Tebuconazole is composite will strengthen the control efficiency of medicament to wheat scab, and in existing disinfectant use in agriculture, both do not carry out mixing using.
Bacteriocide formula of the present invention is by triazolone and Tebuconazole is composite makes, to preventing and treating wheat scab, there is obvious synergistic effect, this synergistic effect not only shows on control sensitive strain, for other group types of field, also has same synergistic effect as carbendazim resistance bacterial strain and triazole type resistant strain.In addition, compare with Tebuconazole single dose, cost can reduce by 25%～30%.This bactericide decapacitation prevents and treats outside wheat scab, can also doublely control wheat powdery mildew, is the biological agricultural chemicals of a kind of efficient, low toxicity, low-residual, environmentally compatible, meets the demand of the nuisanceless production of Agricultural Products.
Bactericide stable chemical nature of the present invention, during mixing, triazolone and Tebuconazole two compositions hold mutually, and chemical reaction does not occur, and of many uses, synergy is remarkable, uses safety.
Bactericide of the present invention has good preventive effect to important disease in wheat scab and Wheat Production, and broad spectrum activity is strong, simultaneously can prevent and treat the carbendazim resistance bacterial strain in field, and can delay pathogen the single dose in formula is developed immunity to drugs.
Embodiment
Embodiment 1 triazolone Tebuconazole built agent and preventing and treating the dull and stereotyped test of pesticide effectiveness of wheat scab
Triazolone and Original Tebuconazole are dissolved in respectively to acetone, then triazolone and Tebuconazole are sneaked into culture matrix by different weight concentration proportionings, measure the inhibition of wheat scab bacteria growing active.The test strain adopting is respectively carbendazim resistance bacterial strain GJ33, Tebuconazole resistant strain DY3-4 and sensitive strain ZJ2-9.The bacterial strain that these several classes are different can represent several large main foreigner tourists of current wheat head blight.Measure the inhibiting rate of medicament to these strain growths, use software DPS to calculate concentration EC in inhibition
50value, this test in triplicate.(the results are shown in Table 1).
This test be take single dose triazolone as standard medicament, and each medicament toxicity index is calculated as follows:
Inhibiting rate (%)=(contrast colony diameter mean-processing colony diameter mean)/(contrast colony diameter mean-bacterium dish diameter) * 100
Relative virus force index TI=(standard medicament EC
50value/reagent agent EC
50value) * 100
Toxicity index (TI) * PB (PA and PB are respectively the percentage composition of each single dose in built agent) of toxicity index (TI) * PA+ single dose B of theoretical toxicity index (the TTI)=single dose A of built agent
The actual toxicity index of built agent (ATI)=(standard medicament EC
50value/mixture EC
50value) * 100
The theoretical toxicity index (TTI) of actual toxicity index (the ATI)/built agent of co-toxicity coefficient CTC={ built agent } * 100
According to above formula, calculate the relative virus force index (the results are shown in Table 2) of each medicament and the co-toxicity index (the results are shown in Table 3) of built agent.
Table 1, triazolone and Tebuconazole and built agent control fusarium graminearum EC thereof
50value
Table 2, triazolone and Tebuconazole and built agent control fusarium graminearum relative virus force index thereof
The co-toxicity of table 3, triazolone and Tebuconazole built agent control gibberella saubinetii
According to Tan Fu outstanding person to the criteria for classifying of co-toxicity coefficient type (Tan Fujie, the bioassay method insect knowledge 198623 (6) of Mixture Use of Insecticides: 279-281), synergistic effect judgement according to being: CTC >=170 are notable synergistic; 120≤CTC≤170 are synergy slightly; 70≤CTC≤120 have summation action; CTC≤70 are antagonism.
By the co-toxicity coefficient calculating, can be found out, triazolone and Tebuconazole are composite with 1: 1 ratio, to preventing and treating wheat scab, there is obvious synergistic effect, effect is better than each single dose, on producing, with triazolone and 1: 1 ratio built agent of Tebuconazole, replacing Tebuconazole single dose uses, can improve bacteriostatic activity, synergy is obvious, and use cost can be reduced to 25%～30%.
Embodiment 2 triazolone Tebuconazole built agents and preventing and treating the in vitro test of pesticide effectiveness of wheat scab
For examination material, be the blooming stage wheat head (black wheat 12), control group and processed group be set, every group of 30 strain wheat heads.Control group clear water moisturizing, chemicals treatment group comprises that triazolone or Tebuconazole single dose field recommend consumption, and the built agent of 1: 1 percentage by weight, and each processed group 30 strain wheat head is dipped in above medicament to 15 seconds, after taking out rapidly, dries in the shade.Again by 1.0 * 10
5gibberella saubinetii spore suspension 10 microlitres be expelled in the little grain husk in wheat head middle part, bagging also use clear water moisturizing, cultivates for 25 ℃ and within 5 days, adds up the incidence of disease, the incidence of disease (%)=(the clever number of falling ill/total clever number) * 100%.Preventive effect (%)=(the chemicals treatment group incidence of disease/control group incidence of disease) * 100%.Result of the test shows, 1: 1 mixed control efficiency to head blight of triazolone and Tebuconazole is better than the control efficiency (table 4) of each single dose.
Table 4, triazolone and Tebuconazole built agent are to the in vitro preventive effect of gibberella saubinetii
Claims (3)
1. the application of bactericide in preventing and treating wheat scab, the active ingredient of described bactericide is triazolone and Tebuconazole, described triazolone and the weight ratio of Tebuconazole are 1:1.
2. application according to claim 1, is characterized in that: the weight percent concentration of described triazolone is 9%～30%.
3. application according to claim 1, is characterized in that: the weight percent concentration of described Tebuconazole is 12.5%～80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010202322.6A CN101836638B (en) | 2010-06-13 | 2010-06-13 | Bactericide for preventing and curing wheat scab |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010202322.6A CN101836638B (en) | 2010-06-13 | 2010-06-13 | Bactericide for preventing and curing wheat scab |
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| Publication Number | Publication Date |
|---|---|
| CN101836638A CN101836638A (en) | 2010-09-22 |
| CN101836638B true CN101836638B (en) | 2014-03-12 |
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|---|---|---|---|
| CN201010202322.6A Expired - Fee Related CN101836638B (en) | 2010-06-13 | 2010-06-13 | Bactericide for preventing and curing wheat scab |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583280A (en) * | 2013-10-18 | 2014-02-19 | 固镇县万佳生态养殖场 | Control method for scab of wheat |
| CN107232205A (en) * | 2017-05-11 | 2017-10-10 | 安徽喜洋洋农业科技有限公司 | A kind of complex preparation and its preparation method for preventing and treating wheat scab |
| CN110846433B (en) * | 2019-11-29 | 2023-03-21 | 湖北省农业科学院植保土肥研究所 | KASP marker related to drug resistance of wheat powdery mildew and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005006420A1 (en) * | 2005-02-12 | 2006-08-31 | Lanxess Deutschland Gmbh | Fungicidal mixtures for wood protection |
| CN101617674B (en) * | 2009-08-14 | 2014-06-18 | 中国农业科学院植物保护研究所 | Bacteria and pest killing compound agent for wheat |
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2010
- 2010-06-13 CN CN201010202322.6A patent/CN101836638B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 三唑酮和多菌灵协同作用对小麦赤霉病菌的增效及机理初探;史建荣等;《江苏农业学报》;19951231;第11卷(第4期);第30-33页 * |
| 史建荣等.三唑酮和多菌灵协同作用对小麦赤霉病菌的增效及机理初探.《江苏农业学报》.1995,第11卷(第4期),第30-33页. |
| 戊唑醇防治小麦赤霉病的田间示范及对籽粒毒素的影响;韩青梅等;《中国植物病理学会2009年学术年会论文集》;20091231;第666-669页 * |
| 韩青梅等.戊唑醇防治小麦赤霉病的田间示范及对籽粒毒素的影响.《中国植物病理学会2009年学术年会论文集》.2009,第666-669页. |
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