CN109105390A - A kind of corn seed cold-proof dose - Google Patents

A kind of corn seed cold-proof dose Download PDF

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Publication number
CN109105390A
CN109105390A CN201811215088.3A CN201811215088A CN109105390A CN 109105390 A CN109105390 A CN 109105390A CN 201811215088 A CN201811215088 A CN 201811215088A CN 109105390 A CN109105390 A CN 109105390A
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China
Prior art keywords
parts
corn seed
seed cold
proof dose
dose according
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CN201811215088.3A
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Chinese (zh)
Inventor
范光发
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Anhui Green Rain Seed Industry Ltd By Share Ltd
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Anhui Green Rain Seed Industry Ltd By Share Ltd
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Priority to CN201811215088.3A priority Critical patent/CN109105390A/en
Publication of CN109105390A publication Critical patent/CN109105390A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
    • A01N41/10Sulfones; Sulfoxides
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to corn seed fields, more particularly to a kind of corn seed cold-proof dose, raw material includes: 10-30 parts of polyethylene glycol, 7-14 parts of synergist, 2-8 parts of dimethyl sulfoxide, 1-4 parts of salicylic acid, 0.5-1.5 parts of calcium chloride, 2-4 parts of emulsifier, 8-10 parts of water by weight.The present invention had not only contained nutritious, but also contains antimicrobial component, can effectively enhance the antibacterial and cold performance of corn seed.

Description

A kind of corn seed cold-proof dose
Technical field
The present invention relates to corn seed fields, and in particular to a kind of corn seed cold-proof dose.
Background technique
Corn is most important crops in the world, accounts for the 20% of grain yield, and the corn seeding area in China is stablized 3 Hundred million mu, occupy second place of the world.Corn is at the second place in China's production estimation, and cultivated area is only second to rice, in recent years Carry out cultivated area also constantly increasing, the status in Chinese national economy and people's lives becomes more and more important.Northeast is that I spends the spring Main maize area, corn are thermophilous, and mean temperature will Severe Reduction lower than 10 DEG C.China northeast spring is windy, temperature It changes greatly, corn is often met with low temperature after early sowing of losing no time in sowing while there is sufficient moisture in the soil, and influences seed sprouting, therefore, it is necessary to a kind of raising seed cold-resistants The cold-proof dose of ability.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of corn seed cold-proof doses.The present invention both contained It is nutritious, and contain antimicrobial component, it can effectively enhance the antibacterial and cold performance of corn seed.
A kind of corn seed cold-proof dose proposed by the present invention, raw material include: 10-30 parts of polyethylene glycol, increase by weight Imitate agent 7-14 parts, 2-8 parts of dimethyl sulfoxide, 1-4 parts of salicylic acid, 0.5-1.5 parts of calcium chloride, 2-4 parts of emulsifier, 8-10 parts of water.
Preferably, the synergist the preparation method comprises the following steps: silver carp fish-skin, pigskin and sea cucumber are uniformly mixed, distillation is added Water adjusts pH to acidity, and primary to stand, filtrate is obtained by filtration in addition pepsin, secondary standing;Filtrate be centrifuged To supernatant, supernatant is hydrolyzed, is freeze-dried, obtains synergist.
Preferably, the silver carp fish-skin, pigskin, sea cucumber, distilled water weight ratio be 1:0.4-0.6:0.1-0.3:65- 70。
Preferably, described to adjust pH to acid concrete operations as using newborn acid for adjusting pH to 1.5-2.5.
Preferably, the time once stood is 40-45h.
Preferably, the additional amount of the pepsin is 55-60U/g.
Preferably, the time of the secondary standing is 20-25h.
Preferably, the centrifugal speed is 6000-8000r/min, centrifugation time 45-55min.
Preferably, the temperature of the hydrolysis is 115-120 DEG C, time 9-11h.
Preferably, the temperature of the freeze-drying is -25 DEG C to -40 DEG C, time 50-65min.
Each raw material is uniformly mixed by the present invention in the preparation.
A kind of corn seed cold-proof dose provided by the invention not only containing nutritious, but also contains antimicrobial component, can effectively enhance The antibacterial and cold performance of corn seed;Synergist is added, silver carp fish-skin, pigskin and the mixture of sea cucumber are extracted, energy Hydroxyproline and the higher collagen hydrolysate liquor of proline content are accessed, due to hydroxyproline and proline synergistic effect, It can stablize structure of biological macromolecule as osmotic adjustment in corn seed cytoplasm, reduce cellular acid, release ammonia Poison, moreover it is possible to adjust intracellular redox point as energy sink, and then effectively improve the cold performance of corn seed, promote Corn seed germination;In addition, hydroxyproline and proline have it is highly hydrophilic, can stablize plasm colloid and tissue in metabolism Process, and then freezing point can be reduced, prevent corn seed cell dehydration;And in its preparation process, first to silver carp fish-skin, pigskin Acid is carried out with the mixture of sea cucumber to mention, then is carried out enzyme and mentioned, two kinds of extracting method synergistic effects, the not only recovery rate of collagen Height, and the content of the γ chain and β chain in collagen is higher, while collagen shows as what porous matrix was interconnected together Reticular structure, there are micron thin layer between Kong Yukong, with the crackle with internal run-through of distribution, make collagen have compared with Good dissolubility and hygroscopicity.In addition, the Sea Cucumber Glycosides contained in sea cucumber extract are inhibited to a variety of fungies, it can Effectively improve anti-microbial property of the invention.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A kind of corn seed cold-proof dose, raw material include: 20 parts of polyethylene glycol, 10 parts of synergist, dimethyl by weight 5 parts of sulfoxide, 2.5 parts of salicylic acid, 1 part of calcium chloride, 3 parts of emulsifier, 9 parts of water.
Embodiment 2
A kind of corn seed cold-proof dose, raw material include: 10 parts of polyethylene glycol, 14 parts of synergist, dimethyl by weight 2 parts of sulfoxide, 4 parts of salicylic acid, 0.5 part of calcium chloride, 4 parts of emulsifier, 8 parts of water;
Wherein, the synergist the preparation method comprises the following steps: silver carp fish-skin, pigskin and sea cucumber are uniformly mixed, distilled water is added, PH is adjusted to acidity, primary to stand, filtrate is obtained by filtration in addition pepsin, secondary standing;Filtrate is centrifuged to obtain Supernatant is freeze-dried by clear liquid, obtains synergist.
Embodiment 3
A kind of corn seed cold-proof dose, raw material include: 30 parts of polyethylene glycol, 7 parts of synergist, dimethyl Asia by weight 8 parts of sulfone, 1 part of salicylic acid, 1.5 parts of calcium chloride, 2 parts of emulsifier, 10 parts of water;
Wherein, the synergist the preparation method comprises the following steps: silver carp fish-skin, pigskin and sea cucumber are uniformly mixed, distilled water is added, PH is adjusted to acidity, primary to stand, filtrate is obtained by filtration in addition pepsin, secondary standing;Filtrate is centrifuged to obtain Supernatant is freeze-dried by clear liquid, obtains synergist;
The silver carp fish-skin, pigskin, sea cucumber, distilled water weight ratio be 1:0.5:0.2:67;
It is described to adjust pH to acid concrete operations as using newborn acid for adjusting pH to 2;
The time once stood is 43h;
The additional amount of the pepsin is 58U/g;
The time of the secondary standing is 23h;
The centrifugal speed is 7000r/min, centrifugation time 50min;
The temperature of the hydrolysis is 117 DEG C, time 10h;
The temperature of the freeze-drying is -35 DEG C, time 60min.
Embodiment 4
A kind of corn seed cold-proof dose, raw material include: 15 parts of polyethylene glycol, 8 parts of synergist, dimethyl Asia by weight 4 parts of sulfone, 2 parts of salicylic acid, 0.8 part of calcium chloride, 2.5 parts of emulsifier, 8.5 parts of water;
Wherein, the synergist the preparation method comprises the following steps: silver carp fish-skin, pigskin and sea cucumber are uniformly mixed, distilled water is added, PH is adjusted to acidity, primary to stand, filtrate is obtained by filtration in addition pepsin, secondary standing;Filtrate is centrifuged to obtain Supernatant is freeze-dried by clear liquid, obtains synergist;
The silver carp fish-skin, pigskin, sea cucumber, distilled water weight ratio be 1:0.4:0.3:65;
It is described to adjust pH to acid concrete operations as using newborn acid for adjusting pH to 2.5;
The time once stood is 40h;
The additional amount of the pepsin is 60U/g;
The time of the secondary standing is 20h;
The centrifugal speed is 8000r/min, centrifugation time 45min;
The temperature of the hydrolysis is 115 DEG C, time 11h;
The temperature of the freeze-drying is -40 DEG C, time 50min.
Embodiment 5
A kind of corn seed cold-proof dose, raw material include: 25 parts of polyethylene glycol, 12 parts of synergist, dimethyl by weight 6 parts of sulfoxide, 3 parts of salicylic acid, 1.3 parts of calcium chloride, 3.5 parts of emulsifier, 9.5 parts of water;
Wherein, the synergist the preparation method comprises the following steps: silver carp fish-skin, pigskin and sea cucumber are uniformly mixed, distilled water is added, PH is adjusted to acidity, primary to stand, filtrate is obtained by filtration in addition pepsin, secondary standing;Filtrate is centrifuged to obtain Supernatant is freeze-dried by clear liquid, obtains synergist;
The silver carp fish-skin, pigskin, sea cucumber, distilled water weight ratio be 1:0.6:0.1:70;
It is described to adjust pH to acid concrete operations as using newborn acid for adjusting pH to 1.5;
The time once stood is 45h;
The additional amount of the pepsin is 55U/g;
The time of the secondary standing is 25h;
The centrifugal speed is 6000r/min, centrifugation time 55min;
The temperature of the hydrolysis is 120 DEG C, time 9h;
The temperature of the freeze-drying is -25 DEG C, time 65min.
Comparative example 1
A kind of corn seed cold-proof dose, raw material include: 25 parts of polyethylene glycol, 6 parts of dimethyl sulfoxide, bigcatkin willow by weight Sour 3 parts, 1.3 parts of calcium chloride, 3.5 parts of emulsifier, 9.5 parts of water.
Test example 1
In identical area, full corn seed 6000 of same batch are randomly choosed, are randomly divided into 6 groups, every group 1000 , it is successively denoted as test group 1-5 and control group, the cold-proof dose obtained respectively with embodiment 1-5 and comparative example 1 is to test group 1-5 It dresses seed, and plants with the corn seed of control group, other management methods are identical, and test group and control group corn seed are cold-proof The seed dressing effect of agent is as follows:
Project Bacteriostasis rate (%) Germination percentage (%) Survival rate (%)
Test group 1 82 99 98
Test group 2 85 100 99
Test group 3 86 99 99
Test group 4 88 100 99
Test group 5 87 100 98
Control group 70 90 91
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (10)

1. a kind of corn seed cold-proof dose, which is characterized in that its raw material includes: 10-30 parts of polyethylene glycol, synergist by weight 7-14 parts, 2-8 parts of dimethyl sulfoxide, 1-4 parts of salicylic acid, 0.5-1.5 parts of calcium chloride, 2-4 parts of emulsifier, 8-10 parts of water.
2. corn seed cold-proof dose according to claim 1, which is characterized in that the synergist the preparation method comprises the following steps: by silver carp Fish fish-skin, pigskin and sea cucumber are uniformly mixed, and distilled water is added, and adjust pH to acidity, pepsin is added in primary standing, secondary It stands, filtrate is obtained by filtration;Filtrate is centrifuged to obtain supernatant, supernatant is hydrolyzed, is freeze-dried, obtains synergy Agent.
3. corn seed cold-proof dose according to claim 2, which is characterized in that the silver carp fish-skin, pigskin, sea cucumber, distillation The weight ratio of water is 1:0.4-0.6:0.1-0.3:65-70.
4. the corn seed cold-proof dose according to Claims 2 or 3, which is characterized in that the pH that adjusts is to acid specific behaviour Newborn acid for adjusting pH is taken as to 1.5-2.5.
5. the corn seed cold-proof dose according to Claims 2 or 3, which is characterized in that the time once stood is 40- 45h。
6. the corn seed cold-proof dose according to Claims 2 or 3, which is characterized in that the additional amount of the pepsin is 55- 60U/g。
7. the corn seed cold-proof dose according to Claims 2 or 3, which is characterized in that the time of the secondary standing is 20- 25h。
8. the corn seed cold-proof dose according to Claims 2 or 3, which is characterized in that the centrifugal speed is 6000-8000r/ Min, centrifugation time 45-55min.
9. the corn seed cold-proof dose according to Claims 2 or 3, which is characterized in that the temperature of the hydrolysis is 115-120 DEG C, time 9-11h.
10. the corn seed cold-proof dose according to Claims 2 or 3, which is characterized in that the temperature of the freeze-drying is -25 DEG C to -40 DEG C, time 50-65min.
CN201811215088.3A 2018-10-18 2018-10-18 A kind of corn seed cold-proof dose Pending CN109105390A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090264291A1 (en) * 1998-02-23 2009-10-22 Etienne Soudant Compositions comprising anti-proliferative agents and use thereof
CN105107011A (en) * 2015-09-11 2015-12-02 江苏振宇环保科技有限公司 Manufacturing method for fish scale collagen hemostatic sponge
CN106359449A (en) * 2016-08-29 2017-02-01 滁州隆扬农业科技有限公司 Corn seed cold-resistant agent
CN108617659A (en) * 2018-04-19 2018-10-09 浦江县欧立生物技术有限公司 Promote the conditioning agent of plant failure cell fast-growth

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090264291A1 (en) * 1998-02-23 2009-10-22 Etienne Soudant Compositions comprising anti-proliferative agents and use thereof
CN105107011A (en) * 2015-09-11 2015-12-02 江苏振宇环保科技有限公司 Manufacturing method for fish scale collagen hemostatic sponge
CN106359449A (en) * 2016-08-29 2017-02-01 滁州隆扬农业科技有限公司 Corn seed cold-resistant agent
CN108617659A (en) * 2018-04-19 2018-10-09 浦江县欧立生物技术有限公司 Promote the conditioning agent of plant failure cell fast-growth

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘金龙: "鱼蛋白多肽的制备及其在农业生产中的应用", 《中国优秀博硕士学位论文全文数据库(硕士) 工程科技Ⅰ辑》 *

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