CN109096520A - 一种改性胶原及其制备方法 - Google Patents

一种改性胶原及其制备方法 Download PDF

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CN109096520A
CN109096520A CN201810697808.8A CN201810697808A CN109096520A CN 109096520 A CN109096520 A CN 109096520A CN 201810697808 A CN201810697808 A CN 201810697808A CN 109096520 A CN109096520 A CN 109096520A
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collagen
modified
modified collagen
epigallo
egcg
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满毅
朱宸佑
向琳
邓佳
屈依丽
刘丽
周寅
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Sichuan University
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
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    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
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Abstract

本发明提供了一种改性胶原，制备方法包括以下步骤：A.由表没食子儿茶素没食子酸酯交联胶原膜；B.加载骨形态发生蛋白‑2至步骤A得到的胶原膜，清洗，干燥，即得。本发明将胶原膜以表没食子儿茶素没食子酸酯改性，再加载骨形态发生蛋白‑2，进而得到促骨再生性能良好的加载了骨形态发生蛋白‑2的表没食子儿茶素没食子酸酯改性胶原复合膜。

Description

一种改性胶原及其制备方法
技术领域
本发明属于生物材料领域，具体涉及一种改性胶原及其制备方法。
背景技术
牙科手术中常需要应用引导骨再生手术进行骨增量。胶原是细胞外基质的一种成分，已 经广泛应用于引导骨再生术中。胶原膜有较好的生物兼容性和较低的抗原性，并能促进成骨 细胞黏附、增殖、迁移和分化，也能有阻挡成纤维细胞快速入侵骨组织生长位点、隔开软组 织与硬组织的生长等屏障功能。
胶原(Collagen)是细胞外最重要的水不溶性纤维蛋白，是构成细胞外基质的骨架。胶原在 哺乳动物体内含量丰富，约占人体或其他动物蛋白的20％-30％。主要存在于软骨、皮肤及肌 腱等组织中，在动物体内起支持、保护及连接等多种作用。由于胶原具有良好的生物相溶性、 无毒、弱抗原性、可降解性及安全性等优点，因此可以作为天然生物材料。胶原膜作为可吸 收性膜，它具有低抗原性，生物相容性好，可参与组织愈合过程，降解速率可根据需要调节 等诸多优点。
表没食子儿茶素没食子酸酯是一种茶多酚，拥有抗癌、抗氧化、抗炎、抗纤维化和促骨 再生的作用。并且，表没食子儿茶素没食子酸酯能提高胶原的热稳定性，并保护胶原不被胶 原酶降解，因此它可以用于胶原膜的交联。
骨形态发生蛋白-2在骨引导、骨再生中有重要作用。骨形态发生蛋白-2通过促进多能干 细胞向骨再生细胞分化，从而得到促骨再生的作用。美国食品与药品监督管理局也批准了相 应的材料可以在临床上使用。
发明内容
本发明的目的是针对现有技术中存在的不足，提供一种改性胶原及其制备方法。
为了达到上述目的，本发明采用了下列技术方案：
一种改性胶原，制备方法包括以下步骤：
A.由表没食子儿茶素没食子酸酯交联胶原膜；
B.加载骨形态发生蛋白-2至步骤A得到的胶原膜，清洗，干燥，即得。
作为优选，所述的胶原膜为牛来源I型胶原制备得到。
作为优选，所述步骤A中，表没食子儿茶素没食子酸酯和胶原膜重量比为1：100。
作为优选，所述步骤A中，交联是将胶原置于表没食子儿茶素没食子酸酯中改性60min，lXPBS缓冲液冲洗。
作为优选，所述步骤A中，胶原膜是在-26℃温度下冷冻过夜；在-53℃、1Pa条件下，真 空冷冻干得到。
作为优选，所述步骤B中，加载为按照100mg：1ml的比例，将胶原膜浸泡在浓度为500ng/ml的骨形态发生蛋白-2的PBS溶液中，浸泡时间为24小时。
作为优选，所述步骤B中，干燥为将制备好的改性胶原膜置于-20℃条件下冷冻干燥过夜。
本发明的优点在于：
本发明将胶原膜以表没食子儿茶素没食子酸酯改性，再加载骨形态发生蛋白-2，进而得到促 骨再生性能良好的加载了骨形态发生蛋白-2的表没食子儿茶素没食子酸酯改性胶原复合膜。 本发明提高了以前胶原膜的生物性能，具有很好的应用前景，有望于应用在引导骨组织再生 手术(GBR)中，为引导再生术等相关增量手术提供了新选择。
具体实施方式
下面，对本发明作详细的说明。
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行 进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定 本发明。
实施例1：一种改性胶原，制备方法包括以下步骤：
A.由表没食子儿茶素没食子酸酯交联胶原膜；
B.加载骨形态发生蛋白-2至步骤A得到的胶原膜，清洗，干燥，即得。
实施例2：一种改性胶原，制备方法包括以下步骤：
A.按照重量比为1：100的比例，将表没食子儿茶素没食子酸酯和牛来源I型胶原制备的胶原 膜交联胶原膜，交联是将牛来源I型胶原置于表没食子儿茶素没食子酸酯中改性60min，lXPBS 缓冲液冲洗；
B.按照100mg：1ml的比例，将胶原膜浸泡在的骨形态发生蛋白-2浓度为500ng/ml的PBS溶 液中，浸泡时间为24小时；清洗，将制备好的改性胶原膜置于-20℃条件下冷冻干燥过夜， 即得。
本发明并不局限于前述的具体实施方式。本发明扩展到任何在本说明书中披露的新特征 或任何新的组合，以及披露的任一新的方法或过程的步骤或任何新的组合。

Claims (7)

1.一种改性胶原，其特征在于制备方法包括以下步骤：
A.由表没食子儿茶素没食子酸酯交联胶原膜；
B.加载骨形态发生蛋白-2至步骤A得到的胶原膜，清洗，干燥，即得。
2.根据权利要求1所述的一种改性胶原，其特征在于：所述的胶原膜为牛来源I型胶原制备得到。
3.根据权利要求1所述的一种改性胶原，其特征在于：所述步骤A中，表没食子儿茶素没食子酸酯和胶原膜的重量比为1：100。
4.根据权利要求1所述的一种改性胶原，其特征在于：所述步骤A中，交联是将胶原置于表没食子儿茶素没食子酸酯中改性60min，lX PBS缓冲液冲洗。
5.根据权利要求1所述的一种改性胶原，其特征在于：所述步骤A中，胶原膜是在-26℃温度下冷冻过夜；在-53℃、1Pa条件下，真空冷冻干得到。
6.根据权利要求1所述的一种改性胶原，其特征在于：所述步骤B中，加载为按照100mg：1ml的比例，将胶原膜浸泡在浓度为500ng/ml的骨形态发生蛋白-2的PBS溶液中，浸泡时间为24小时。
7.根据权利要求1所述的一种改性胶原，其特征在于：所述步骤B中，干燥为将制备好的改性胶原膜置于-20℃条件下冷冻干燥过夜。
CN201810697808.8A 2018-06-29 2018-06-29 一种改性胶原及其制备方法 Pending CN109096520A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114191618A (zh) * 2021-06-01 2022-03-18 四川大学华西医院 一种减缓纤维化和炎性巨噬细胞募集的改性骨修复材料

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101496914A (zh) * 2008-02-03 2009-08-05 烟台正海生物技术有限公司 骨支架材料及其制备方法与应用
CN102225219A (zh) * 2011-06-01 2011-10-26 陕西博鸿生物科技有限公司 一种骨组织再生引导膜及其制备方法
CN106390195A (zh) * 2016-11-16 2017-02-15 四川大学 一种胶原膜的改性方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101496914A (zh) * 2008-02-03 2009-08-05 烟台正海生物技术有限公司 骨支架材料及其制备方法与应用
CN102225219A (zh) * 2011-06-01 2011-10-26 陕西博鸿生物科技有限公司 一种骨组织再生引导膜及其制备方法
CN106390195A (zh) * 2016-11-16 2017-02-15 四川大学 一种胶原膜的改性方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114191618A (zh) * 2021-06-01 2022-03-18 四川大学华西医院 一种减缓纤维化和炎性巨噬细胞募集的改性骨修复材料

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Application publication date: 20181228