CN109089807A - A kind of implantation methods of environmental protection and public nuisance free rice - Google Patents
A kind of implantation methods of environmental protection and public nuisance free rice Download PDFInfo
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- CN109089807A CN109089807A CN201811003575.3A CN201811003575A CN109089807A CN 109089807 A CN109089807 A CN 109089807A CN 201811003575 A CN201811003575 A CN 201811003575A CN 109089807 A CN109089807 A CN 109089807A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0237—Devices for protecting a specific part of a plant, e.g. roots, trunk or fruits
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Abstract
The invention discloses a kind of implantation methods of environmental protection and public nuisance free rice, it is characterized in that, the following steps are included: culture photosynthetic bacteria-, which prepares the obtained base fluid-of photosynthetic bacterium solution a-, is mixed to prepare photosynthetic bacterium solution b for photosynthetic bacterium solution a and base fluid, and when rice moves seedling, rice shoot root system is immersed in 12~14h in photosynthetic bacterium solution b, photosynthetic bacteria rhizosphere film is formed in rice shoot rhizosphere, is then planted.Compared with prior art; the invention has the following advantages: (1) present invention forms rhizosphere mycoderm in soil root using the photosynthetic bacteria of improvement; rice root is protected; check the entrance of mercury; reduce the content of mercury in rice seed; more significant compared to traditional rhizosphere spray application effect, amount of application is few, the ability with excellent barrier rice Adsorption of Mercury；(2) present invention is environmentally protective, small to the influence power of the original ecosystem of soil and farmland yield while effectively barrier rice Adsorption of Mercury, and can promote microbial populations in soil abundance.
Description
Technical field
The present invention relates to a kind of implantation methods of environmental protection and public nuisance free rice, and in particular to one kind is hindered by photosynthetic bacteria rhizosphere film
Hold back rice absorbing mercury element, and then reach the method for production environmental protection and public nuisance free rice, belongs to field of agricultural cultivation.
Background technique
Mercury is a kind of high toxicity, persistent heavy metal contaminants, is classified as one of primary control pollutant by national governments.
Instantly due to the activity of the mankind and the promotion of industrialized degree, a large amount of mercury element is entered in soil.Partial soil is also
The crops such as wheat, rice have been planted behind field.By the predation of food chain, there is very big a part that can enter human body, danger
Evil human health.So the improvement of the mercury element in soil becomes the task of top priority of environmental improvement instantly.Common inorganic mercury
Compound has mercury bichloride (mercuric chloride), mercury chloride (calomel), mercury fulminate, mercuric nitrate, mercuric arsenate and mercury cyanide etc..It is producing and is using
Mercury mainly enters human body through respiratory tract in the form of steam in the process, as mercury ore smelting, gold-plating, pearl sand dry, gas welding cutting belt mercury
Equipment etc..Gastrointestinal tract absorption mercury metal is little, does not cause generally to be poisoned.Liveliness proof acute poisoning be mostly oral mercuric chloride, calomel and
Caused by the mercury compounds such as mercury fulminate, it is main performance that inorganic mercury poisoning, which is with alimentary canal and kidney damage, and common poisons are mercury chloride,
Its lethal dose is only about 1g.Therefore rice of ingesting is likely to become one of mercury pollution area crowd's mercury chloride exposure main path, prestige
Coerce local resident's health.
Control measures of the modern science and technology to the inorganic mercury pollution of field soil: heat treatment technics, leaching recovery technique, fixation
Change technology, electric repairing technique, phytoremediation technology, gene repair technology and nanometer recovery technique etc..Wherein it is heat-treated skill
Art, leaching recovery technique, electric repairing technique can cause soil to lose arability；Its method of immobilization technology is simple to operation,
And a possibility that mercury in soils exposure can be effectively reduced, but applying immobilization medicament influences even destroy field ecology system
System, medicament are much larger than the harm of inorganic mercury to the influence of the ecosystem；In addition, the applied amount of immobilization medicament is also difficult to control,
Its applying mode is difficult to operate, and will cause the accumulation of other heavy metals.In soil-plant system, research report is used
Sodium selenate or sodium selenite medicament can effectively inhibit accumulation of the inorganic mercury in radish, tomato, leaf mustard, soybean, green onion and garlic.
But apply selenium amount and be difficult to control in the actual operation process, easily cause selenium excessive.
Therefore, the present invention proposes a kind of implantation methods of environmental protection and public nuisance free rice, and in particular to one kind passes through photosynthetic mycorhiza
Border film checks rice absorbing mercury element, and then reaches the method for production non-pollution rice.
Summary of the invention
The technical problems to be solved by the present invention are: for being inhibited in current Rice Cropping using curing agent/stabilizer
The method of mercury accumulation, but due to this method curing agent/stabilizer complicated component, and administration dosage is difficult to the problems such as holding, easily
The original ecosystem of soil is destroyed, farmland yield is impacted.
In order to solve the above technical problems, the present invention provides technical solution below:
The implantation methods of environmental protection and public nuisance free rice of the invention, which comprises the following steps:
A. it takes 0.5~1.5 part of photosynthetic bacteria and 4~6 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A work
The photosynthetic bacterium solution a of bacterium number/mL.
B. it takes 4~6 parts of starch and 0.5~1.5 part of cold water mix uniform by weight, adds 3.5~4.5 parts of temperature
It is uniformly mixed for 75~85 DEG C of hot water, base fluid is made after being cooled to room temperature.
C. by weight it is that 1:0.8~1.2 mix by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirs
It mixes uniformly, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12~14h in photosynthetic bacterium solution b, in rice shoot root
Border forms photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Further, rice plant of tillering stage root spray 9~11 times of photosynthetic bacterium solution a dilution, fountain height be 10~
20Kg/ mus.
Further, the cultural method of the photosynthetic bacteria is as follows:
A, 90~100 parts of distilled water, 30~35 parts of beef extract, 10 ~ 15 parts of glucose, 22~26 parts of sodium chloride, phosphorus are taken by weight
4~5 parts of sour hydrogen dipotassium, 4~5 parts of potassium dihydrogen phosphate, 3~5 parts of magnesium chloride, 0.1~0.3 part of N-methyl-N-nitrosourea, mixing
It stirs evenly, and 15~25min of sterilizing at 115~126 DEG C, obtains culture medium.
B, the photosynthetic bacteria strain of 8~9 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 2.5~3.5 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 29~33 DEG C, with 110~
The speed of 130rpm carries out shake culture, and incubation time is 20~24 hours.
Above-mentioned straw powder is stalk to pulverize and sieve for 80~150 mesh powders.
C, after the completion of step b, 2~3 weight starch is added, stir evenly, stand 40~50min, after solution clarification
Photosynthetic bacteria can be prepared by taking the clarified solution on upper layer.
Further, the photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and described
Purple sulfur bacteria, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:0.5 ~ 4:0.5 ~ 3.
Compared with prior art, the invention has the following advantages:
(1) present invention forms rhizosphere mycoderm in soil root using the photosynthetic bacteria of improvement, protects to rice root, checks mercury
Entrance, reduce rice seed in mercury content, more significant compared to traditional rhizosphere spray application effect, amount of application is few, has
The ability of excellent barrier rice Adsorption of Mercury.
(2) present invention is environmentally protective, while effectively barrier rice Adsorption of Mercury, to the original ecosystem of soil and agriculture
The influence power of field yield is small, and can promote microbial populations in soil abundance.
Detailed description of the invention
Fig. 1 is 16 subordinate list of embodiment.
Specific embodiment
The following detailed description of the preferred embodiment of the present invention.
Embodiment 1: the following steps are included:
Cultivate photosynthetic bacteria:
A. 90 parts of distilled water, 35 parts of beef extract, 10 parts of glucose, 22.5 parts of sodium chloride, 4 parts of dipotassium hydrogen phosphate, phosphorus are taken by weight
5 parts of acid dihydride potassium, 4 parts of magnesium chloride, 0.1 part of N-methyl-N-nitrosourea, are mixed evenly, and the sterilizing at 126 DEG C
15min obtains culture medium.
B. the photosynthetic bacteria strain of 8 parts by weight is inoculated with into the culture medium of step a, and 3 weights are added in culture medium after inoculation
Measure part straw powder, stir evenly, be placed in constant incubator, set temperature be 29 DEG C, with the speed of 115rpm into
Row shake culture, incubation time are 20 hours.
It is 80 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:0.5:0.5.
C. after the completion of step b, 2 weight starch is added, stir evenly, stand 50min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 0.5 part of photosynthetic bacteria and 6 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL's
Photosynthetic bacterium solution a.
B. it takes 6 parts of starch and 1.5 parts of cold water mix uniform by weight, adds the hot water that 3.5 parts of temperature are 75 DEG C
It is uniformly mixed, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:1.2 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12h in photosynthetic bacterium solution b, forms light in rice shoot rhizosphere
Combined bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 9 times of photosynthetic bacterium solution a in root, and fountain height is 10Kg/ mus.
Embodiment 2: the following steps are included:
Cultivate photosynthetic bacteria:
A. take by weight 91 parts of distilled water, 34.5 parts of beef extract, 15 parts of glucose, 22 parts of sodium chloride, 4.1 parts of dipotassium hydrogen phosphate,
4.9 parts of potassium dihydrogen phosphate, 5 parts of magnesium chloride, 0.12 part of N-methyl-N-nitrosourea, are mixed evenly, and kill at 125 DEG C
Bacterium sterilizes 16min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.1 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 3.1 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 30 DEG C, with the rotary frequency of 120rpm
Rate carries out shake culture, and incubation time is 24 hours.
It is 85 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:4:3.
C. after the completion of step b, 2.1 weight starch is added, stir evenly, stand 40min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 0.6 part of photosynthetic bacteria and 5.8 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 5.5 parts of starch and 1.4 parts of cold water mix uniform by weight, adds the heat that 3.6 parts of temperature are 76 DEG C
Water is uniformly mixed, and base fluid is made after being cooled to room temperature.
It C. is by weight that 1:1.1 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 13h in photosynthetic bacterium solution b, forms light in rice shoot rhizosphere
Combined bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 10 times of photosynthetic bacterium solution a in root, and fountain height is 11Kg/ mus.
Embodiment 3: the following steps are included:
Cultivate photosynthetic bacteria:
A. take by weight 92 parts of distilled water, 34 parts of beef extract, 14 parts of glucose, 23.5 parts of sodium chloride, 4.2 parts of dipotassium hydrogen phosphate,
4.8 parts of potassium dihydrogen phosphate, 4.2 parts of magnesium chloride, 0.14 part of N-methyl-N-nitrosourea, are mixed evenly, and at 124 DEG C
Sterilizing 17min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.2 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 3.2 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 31 DEG C, with the rotary frequency of 112rpm
Rate carries out shake culture, and incubation time is 20.5 hours.
It is 90 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:0.5:3.
C. after the completion of step b, 2.2 weight starch is added, stir evenly, stand 49min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 0.7 part of photosynthetic bacteria and 5.6 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 4.5 parts of starch and 1.3 parts of cold water mix uniform by weight, adds the heat that 3.7 parts of temperature are 77 DEG C
Water is uniformly mixed, and base fluid is made after being cooled to room temperature.
C. by weight it is that 1:1 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirs evenly,
Photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 14h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere photosynthetic
Mycorhiza border film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 11 times of photosynthetic bacterium solution a in root, and fountain height is 12Kg/ mus.
Embodiment 4: the following steps are included:
Cultivate photosynthetic bacteria:
A. take by weight 93 parts of distilled water, 33.5 parts of beef extract, 13 parts of glucose, 23 parts of sodium chloride, 4.3 parts of dipotassium hydrogen phosphate,
4.7 parts of potassium dihydrogen phosphate, 4.4 parts of magnesium chloride, 0.16 part of N-methyl-N-nitrosourea, are mixed evenly, and at 123 DEG C
Sterilizing 18min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.3 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 3.3 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 32 DEG C, with the rotary frequency of 114rpm
Rate carries out shake culture, and incubation time is 23.5 hours.
It is 95 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:4:0.5.
C. after the completion of step b, 2.3 weight starch is added, stir evenly, stand 48min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 0.8 part of photosynthetic bacteria and 5.4 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 6 parts of starch and 1.2 parts of cold water mix uniform by weight, adds the hot water that 3.8 parts of temperature are 78 DEG C
It is uniformly mixed, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:0.9 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12.5h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 9 times of photosynthetic bacterium solution a in root, and fountain height is 13Kg/ mus.
Embodiment 5: the following steps are included:
Cultivate photosynthetic bacteria:
A. take by weight 94 parts of distilled water, 33 parts of beef extract, 12 parts of glucose, 24.5 parts of sodium chloride, 4.4 parts of dipotassium hydrogen phosphate,
4.6 parts of potassium dihydrogen phosphate, 4.6 parts of magnesium chloride, 0.18 part of N-methyl-N-nitrosourea, are mixed evenly, and at 122 DEG C
Sterilizing 19min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.4 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 3.4 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 33 DEG C, with the rotary frequency of 116rpm
Rate carries out shake culture, and incubation time is 21 hours.
It is 100 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:1:1.
C. after the completion of step b, 2.4 weight starch is added, stir evenly, stand 47min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 0.9 part of photosynthetic bacteria and 5.2 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 5.8 parts of starch and 1.1 parts of cold water mix uniform by weight, adds the heat that 3.9 parts of temperature are 79 DEG C
Water is uniformly mixed, and base fluid is made after being cooled to room temperature.
It C. is by weight that 1:0.8 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 13.5h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 10 times of photosynthetic bacterium solution a in root, and fountain height is 14Kg/ mus.
Embodiment 6: the following steps are included:
Cultivate photosynthetic bacteria:
A. take by weight 95 parts of distilled water, 32.5 parts of beef extract, 11 parts of glucose, 24 parts of sodium chloride, 4.5 parts of dipotassium hydrogen phosphate,
4.5 parts of potassium dihydrogen phosphate, 4.7 parts of magnesium chloride, 0.2 part of N-methyl-N-nitrosourea, are mixed evenly, and kill at 121 DEG C
Bacterium sterilizes 20min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.5 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 3.5 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 29.5 DEG C, with the rotation of 118rpm
Frequency carries out shake culture, and incubation time is 23 hours.
It is 105 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:1:1.5.
C. after the completion of step b, 2.5 weight starch is added, stir evenly, stand 46min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1 part of photosynthetic bacteria and 5 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109The light of a viable count/mL
Combined bacteria liquid a.
B. it takes 5.6 parts of starch and 1 part of cold water mix uniform by weight, it is mixed to add the hot water that 4 parts of temperature are 80 DEG C
It closes uniformly, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:0.8 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12.2h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 11 times of photosynthetic bacterium solution a in root, and fountain height is 15Kg/ mus.
Embodiment 7: the following steps are included:
Cultivate photosynthetic bacteria:
A. 96 parts of distilled water, 32 parts of beef extract, 10.5 parts of glucose, 25.5 parts of sodium chloride, dipotassium hydrogen phosphate 4.6 are taken by weight
Part, 4.4 parts of potassium dihydrogen phosphate, 4.8 parts of magnesium chloride, 0.22 part of N-methyl-N-nitrosourea, are mixed evenly, and at 120 DEG C
Lower sterilizing 21min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.6 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 2.9 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 30.5 DEG C, with the rotation of 130rpm
Frequency carries out shake culture, and incubation time is 21.5 hours.
It is 110 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:1:2.
C. after the completion of step b, 2.6 weight starch is added, stir evenly, stand 45min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1.1 parts of photosynthetic bacteria and 4.8 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 5.4 parts of starch and 0.9 part of cold water mix uniform by weight, adds the heat that 4.1 parts of temperature are 81 DEG C
Water is uniformly mixed, and base fluid is made after being cooled to room temperature.
It C. is by weight that 1:0.9 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12.4h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 9 times of photosynthetic bacterium solution a in root, and fountain height is 16Kg/ mus.
Embodiment 8: the following steps are included:
Cultivate photosynthetic bacteria:
A. 97 parts of distilled water, 31.5 parts of beef extract, 11.5 parts of glucose, 25 parts of sodium chloride, dipotassium hydrogen phosphate 4.7 are taken by weight
Part, 4.3 parts of potassium dihydrogen phosphate, 3.8 parts of magnesium chloride, 0.24 part of N-methyl-N-nitrosourea, are mixed evenly, and at 119 DEG C
Lower sterilizing 22min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.7 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 2.8 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 31.5 DEG C, with the rotation of 128rpm
Frequency carries out shake culture, and incubation time is 22.5 hours.
It is 115 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:1:2.5.
C. after the completion of step b, 2.7 weight starch is added, stir evenly, stand 44min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1.2 parts of photosynthetic bacteria and 4.6 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 5.2 parts of starch and 0.8 part of cold water mix uniform by weight, adds the heat that 4.2 parts of temperature are 82 DEG C
Water is uniformly mixed, and base fluid is made after being cooled to room temperature.
C. by weight it is that 1:1 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirs evenly,
Photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12.6h in photosynthetic bacterium solution b, forms light in rice shoot rhizosphere
Combined bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 10 times of photosynthetic bacterium solution a in root, and fountain height is 17Kg/ mus.
Embodiment 9: the following steps are included:
Cultivate photosynthetic bacteria:
A. take by weight 98 parts of distilled water, 31 parts of beef extract, 12.5 parts of glucose, 26 parts of sodium chloride, 4.8 parts of dipotassium hydrogen phosphate,
4.2 parts of potassium dihydrogen phosphate, 3.6 parts of magnesium chloride, 0.26 part of N-methyl-N-nitrosourea, are mixed evenly, and at 120 DEG C
Sterilizing 23min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.8 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 2.7 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 32.5 DEG C, with the rotation of 126rpm
Frequency carries out shake culture, and incubation time is 22 hours.
It is 120 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:1:3.
C. after the completion of step b, 2.8 weight starch is added, stir evenly, stand 43min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1.3 parts of photosynthetic bacteria and 4.4 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 5 parts of starch and 0.7 part of cold water mix uniform by weight, adds the hot water that 4.3 parts of temperature are 83 DEG C
It is uniformly mixed, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:1.1 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12.8h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 11 times of photosynthetic bacterium solution a in root, and fountain height is 18Kg/ mus.
Embodiment 10: the following steps are included:
Cultivate photosynthetic bacteria:
A. 99 parts of distilled water, 30.5 parts of beef extract, 13.5 parts of glucose, 22 parts of sodium chloride, dipotassium hydrogen phosphate 4.9 are taken by weight
Part, 4.1 parts of potassium dihydrogen phosphate, 3.4 parts of magnesium chloride, 0.28 part of N-methyl-N-nitrosourea, are mixed evenly, and at 121 DEG C
Lower sterilizing 24min, obtains culture medium.
B. the photosynthetic bacteria strain of 8.9 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 2.6 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 33 DEG C, with 110~130rpm's
Speed carries out shake culture, and incubation time is 20 hours.
It is 125 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:2:3.
C. after the completion of step b, 2.9 weight starch is added, stir evenly, stand 42min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1.4 parts of photosynthetic bacteria and 4.2 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 4.8 parts of starch and 0.6 part of cold water mix uniform by weight, adds the heat that 4.4 parts of temperature are 84 DEG C
Water is uniformly mixed, and base fluid is made after being cooled to room temperature.
It C. is by weight that 1:1.2 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 13.2h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 9 times of photosynthetic bacterium solution a in root, and fountain height is 19Kg/ mus.
Embodiment 11: the following steps are included:
Cultivate photosynthetic bacteria:
A. 100 parts of distilled water, 30 parts of beef extract, 14.5 parts of glucose, 23 parts of sodium chloride, 5 parts of dipotassium hydrogen phosphate, phosphorus are taken by weight
4 parts of acid dihydride potassium, 3.2 parts of magnesium chloride, 0.3 part of N-methyl-N-nitrosourea, are mixed evenly, and sterilize and disappear at 122 DEG C
Malicious 25min, obtains culture medium.
B. the photosynthetic bacteria strain of 9 parts by weight is inoculated with into the culture medium of step a, is added 2.5 in culture medium after inoculation
The straw powder of parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 29 DEG C, with the speed of 124rpm
Shake culture is carried out, incubation time is 21 hours.
It is 130 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:3:2.
C. after the completion of step b, 3 weight starch is added, stir evenly, stand 41min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1.5 parts of photosynthetic bacteria and 4 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL's
Photosynthetic bacterium solution a.
B. it takes 4.6 parts of starch and 0.5 part of cold water mix uniform by weight, adds the heat that 4.5 parts of temperature are 85 DEG C
Water is uniformly mixed, and base fluid is made after being cooled to room temperature.
It C. is by weight that 1:0.8 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 13.4h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 10 times of photosynthetic bacterium solution a in root, and fountain height is 20Kg/ mus.
Embodiment 12: the following steps are included:
Cultivate photosynthetic bacteria:
A. 90 parts of distilled water, 31 parts of beef extract, 15 parts of glucose, 24 parts of sodium chloride, 4.5 parts of dipotassium hydrogen phosphate, phosphorus are taken by weight
4.5 parts of acid dihydride potassium, 3 parts of magnesium chloride, 0.15 part of N-methyl-N-nitrosourea, are mixed evenly, and sterilize at 123 DEG C
15min is sterilized, culture medium is obtained.
B. the photosynthetic bacteria strain of 8.8 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 2.8 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 30 DEG C, with the rotary frequency of 122rpm
Rate carries out shake culture, and incubation time is 22 hours.
It is 135 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:4:1.5.
C. after the completion of step b, 2 weight starch is added, stir evenly, stand 40min, take upper layer after solution clarification
Clarified solution can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 0.5 part of photosynthetic bacteria and 4.5 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 4.4 parts of starch and 0.5 part of cold water mix uniform by weight, adds the hot water that 3 parts of temperature are 75 DEG C
It is uniformly mixed, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:0.85 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 13.6h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 11 times of photosynthetic bacterium solution a in root, and fountain height is 10.5Kg/ mus.
Embodiment 13: the following steps are included:
Cultivate photosynthetic bacteria:
A. 95 parts of distilled water, 32 parts of beef extract, 10 parts of glucose, 25 parts of sodium chloride, 4.8 parts of dipotassium hydrogen phosphate, phosphorus are taken by weight
4.7 parts of acid dihydride potassium, 4 parts of magnesium chloride, 0.25 part of N-methyl-N-nitrosourea, are mixed evenly, and sterilize at 124 DEG C
25min is sterilized, culture medium is obtained.
B. the photosynthetic bacteria strain of 8.6 parts by weight is inoculated with into the culture medium of step a, is added 3 in culture medium after inoculation
The straw powder of parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 31 DEG C, with the speed of 120rpm
Shake culture is carried out, incubation time is 23 hours.
It is 140 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:0.5:2.
C. after the completion of step b, 2.2 weight starch is added, stir evenly, stand 45.5min, taken after solution clarification
The clarified solution of layer can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1.5 parts of photosynthetic bacteria and 5.5 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL
Photosynthetic bacterium solution a.
B. it takes 4.2 parts of starch and 1.5 parts of cold water mix uniform by weight, adds the hot water that 4 parts of temperature are 79 DEG C
It is uniformly mixed, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:0.95 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 13.8h in photosynthetic bacterium solution b, is formed in rice shoot rhizosphere
Photosynthetic bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 9 times of photosynthetic bacterium solution a in root, and fountain height is 12.5Kg/ mus.
Embodiment 14: the following steps are included:
Cultivate photosynthetic bacteria:
A. 96 parts of distilled water, 33 parts of beef extract, 12 parts of glucose, 26 parts of sodium chloride, 4.2 parts of dipotassium hydrogen phosphate, phosphorus are taken by weight
4.1 parts of acid dihydride potassium, 4.5 parts of magnesium chloride, 0.2 part of N-methyl-N-nitrosourea, are mixed evenly, and sterilize at 125 DEG C
18min is sterilized, culture medium is obtained.
B. the photosynthetic bacteria strain of 8.4 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 2.5 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 32 DEG C, with the rotary frequency of 125rpm
Rate carries out shake culture, and incubation time is 24 hours.
It is 145 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:0.5:1.
C. after the completion of step b, 2.5 weight starch is added, stir evenly, stand 42.5min, taken after solution clarification
The clarified solution of layer can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 0.5 part of photosynthetic bacteria and 4 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable count/mL's
Photosynthetic bacterium solution a.
B. it takes 6 parts of starch and 1 part of cold water mix uniform by weight, it is mixed to add the hot water that 4.5 parts of temperature are 80 DEG C
It closes uniformly, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:1.05 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 14h in photosynthetic bacterium solution b, forms light in rice shoot rhizosphere
Combined bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 10 times of photosynthetic bacterium solution a in root, and fountain height is 14.5Kg/ mus.
Embodiment 15: the following steps are included:
Cultivate photosynthetic bacteria:
A. 93 parts of distilled water, 34 parts of beef extract, 13 parts of glucose, 24 parts of sodium chloride, 4.5 parts of dipotassium hydrogen phosphate, phosphorus are taken by weight
4.9 parts of acid dihydride potassium, 3.5 parts of magnesium chloride, 0.1 part of N-methyl-N-nitrosourea, are mixed evenly, and sterilize at 126 DEG C
20min is sterilized, culture medium is obtained.
B. the photosynthetic bacteria strain of 8.2 parts by weight is inoculated with into the culture medium of step a, is added in culture medium after inoculation
The straw powder of 3.5 parts by weight, stirs evenly, and is placed in constant incubator, and set temperature is 33 DEG C, with the rotary frequency of 130rpm
Rate carries out shake culture, and incubation time is 22.5 hours.
It is 150 mesh powders that the straw powder, which is by stalk crushing, spare after sieving.
The photosynthetic bacteria strain is the Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulphur is thin
Bacterium, cyanobacterium and green sulfur bacteria are mixed according to the ratio of 1:0.5:2.5.
C. after the completion of step b, 2.8 weight starch is added, stir evenly, stand 47.5min, taken after solution clarification
The clarified solution of layer can be prepared into photosynthetic bacteria.
Plant environmental protection and public nuisance free rice:
A. it takes 1 part of photosynthetic bacteria and 6 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109The light of a viable count/mL
Combined bacteria liquid a.
B. it takes 4 parts of starch and 0.5 part of cold water mix uniform by weight, it is mixed to add the hot water that 4 parts of temperature are 80 DEG C
It closes uniformly, base fluid is made after being cooled to room temperature.
It C. is by weight that 1:1.15 is mixed by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12h in photosynthetic bacterium solution b, forms light in rice shoot rhizosphere
Combined bacteria rhizosphere film, subsequent rice cultivation.
Rice plant of tillering stage, sprays the dilution of 11 times of photosynthetic bacterium solution a in root, and fountain height is 16.5Kg/ mus.
Embodiment 16: verifying embodiment is compareed for Rice Cropping.
It is divided into 5 groups to be verified, 1-4 group is 4 test groups, is planted in the present inventive method, and the 5th group is pair
According to group, plant according to a conventional method.The concentration of 4 groups of mercury in soils pollutants experimental field is 40mg/Kg or more.
Respectively in seedling stage, tillering stage, heading flowering period and grouting parameter extract the corresponding site of test plant, as root,
Leaf, seed are detected, and the content of the life mercury metal of each test group is obtained.
Analyze result such as subordinate list (referring to Fig. 1).
This experimental technique has the excellent ability for checking rice absorbing mercury known to subordinate list.
Specific test is as follows.
One, photosynthetic bacteria rhizosphere film liquid is prepared by following process.
A. according to parts by weight, 90~100 parts of distilled water, 30~35 portions of beef extracts, 10 ~ 15 parts of glucose, 22~26 are taken
Part sodium chloride, 4~5 parts of dipotassium hydrogen phosphates, 4~5 parts of potassium dihydrogen phosphates, 3~5 parts of magnesium chlorides, 0.1~0.3 part of N- methyl-N- are sub-
Nitrourea stirs evenly, and sterilizing, obtains culture medium；
B. 8 ~ 9 parts of photosynthetic bacteria strains are inoculated with into culture medium, and be placed in constant temperature for 3 parts of straw powders are added in the culture medium after inoculation
In incubator, set temperature is 29~33 DEG C, and with 120rpm shake culture, when culture is 20 ~ 24 hours a length of；
C. after cultivating, 2 ~ 3 parts of starch is added, stir evenly, stand 40~50min, light can be prepared into after solution clarification
Combined bacteria bacterium solution.Three kinds of Mixed Microbes of photosynthetic bacteria strain selection purple sulfur bacteria, cyanobacterium, green sulfur bacteria.
Two, the Rice Cropping of three test groups is as follows.
Test group 1:
(1) 1 part of photosynthetic bacteria is mixed with 5 parts of water, activation culture 20h is configured to 5.2 × 109The bacterium solution of a viable count/mL
a；5 parts of starch and 1 part of cold water are mixed, 4 parts of hot water is added and mixes formation base fluid b after cooling；By 1 part of bacterium solution a and 1 part of base
Liquid b mixes to form photosynthetic bacterium solution c；When rice moves seedling, 100 parts of rice shoot root systems are immersed in 12h in photosynthetic bacterium solution c, in rice shoot
Rhizosphere forms photosynthetic bacteria rhizosphere film, rice cultivation after immersion；
(2) 10 times of diluted photosynthetic bacterium solution a are sprayed in rice plant of tillering stage, fountain height is that 12Kg, sprinkling position are plant roots per acre
Portion.The root, leaf and seed for acquiring the rice in each period respectively are detected.
Test group 2:
(1) 1 part of photosynthetic bacteria is mixed with 5 parts of water, activation culture 23h is configured to 7.0 × 109The bacterium solution of a viable count/mL
a；5 parts of starch and 1 part of cold water are mixed, 4 parts of hot water is added and mixes formation base fluid b after cooling；By 1 part of bacterium solution a and 1 part of base
Liquid b mixes to form photosynthetic bacterium solution c；When rice moves seedling, 100 parts of rice shoot root systems are immersed in 13h in photosynthetic bacterium solution c, in rice shoot
Rhizosphere forms photosynthetic bacteria rhizosphere film, rice cultivation after immersion；
(2) 10 times of diluted photosynthetic bacterium solution a are sprayed in rice plant of tillering stage, fountain height is that 15Kg, sprinkling position are plant roots per acre
Portion.The root, leaf and seed for acquiring the rice in each period respectively are detected.
Test group 3:
(1) 1 part of photosynthetic bacteria is mixed with 5 parts of water, activation culture for 24 hours, is configured to 9.0 × 109The bacterium solution of a viable count/mL
a；5 parts of starch and 1 part of cold water are mixed, 4 parts of hot water is added and mixes formation base fluid b after cooling；By 1 part of bacterium solution a and 1 part of base
Liquid b mixes to form photosynthetic bacterium solution c；When rice moves seedling, 100 parts of rice shoot root systems are immersed in 14h in photosynthetic bacterium solution c, in rice shoot
Rhizosphere forms photosynthetic bacteria rhizosphere film, rice cultivation after immersion；
(2) 10 times of diluted photosynthetic bacterium solution a are sprayed in rice plant of tillering stage, fountain height is that 17Kg, sprinkling position are plant roots per acre
Portion.The root, leaf and seed for acquiring the rice in each period respectively are detected.
Test group 4:
(1) 1 part of photosynthetic bacteria is mixed with 5 parts of water, activation culture for 24 hours, is configured to 9.6 × 109The bacterium solution of a viable count/mL
a；5 parts of starch and 1 part of cold water are mixed, 4 parts of hot water is added and mixes formation base fluid b after cooling；By 1 part of bacterium solution a and 1 part of base
Liquid b mixes to form photosynthetic bacterium solution c；When rice moves seedling, 100 parts of rice shoot root systems are immersed in 14h in photosynthetic bacterium solution c, in rice shoot
Rhizosphere forms photosynthetic bacteria rhizosphere film, rice cultivation after immersion；
(2) 10 times of diluted photosynthetic bacterium solution a are sprayed in rice plant of tillering stage, fountain height is that 20Kg, sprinkling position are plant roots per acre
Portion.The root, leaf and seed for acquiring the rice in each period respectively are detected.
Control group: it plants according to a conventional method.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art
For, without departing from the concept of the premise of the invention, several changes and improvements can also be made, these belong to the present invention
Protection scope.
Claims (7)
1. a kind of implantation methods of environmental protection and public nuisance free rice, which is characterized in that mainly comprise the steps that
A. it takes 0.5~1.5 part of photosynthetic bacteria and 4~6 parts of water to be uniformly mixed by weight, is configured to 5.2~9.6 × 109A viable bacteria
The photosynthetic bacterium solution a of number/mL；
B. take 4~6 parts of starch and 0.5~1.5 part of cold water mix uniform by weight, adding 3.5~4.5 parts of temperature is 75
~85 DEG C of hot water is uniformly mixed, and base fluid is made after being cooled to room temperature；
It C. is by weight that 1:0.8~1.2 mix by the base fluid of the photosynthetic bacterium solution a of above-mentioned steps A and step B, stirring is equal
It is even, photosynthetic bacterium solution b is made, when rice moves seedling, rice shoot root system is immersed in 12~14h in photosynthetic bacterium solution b, in rice shoot rhizosphere shape
At photosynthetic bacteria rhizosphere film, then planted.
2. the implantation methods of environmental protection and public nuisance free rice as described in claim 1, which is characterized in that rice plant of tillering stage is sprayed in root
The dilution of 9~11 times of photosynthetic bacterium solution a is spilt, fountain height is 10~20Kg/ mus.
3. the implantation methods of environmental protection and public nuisance free rice as claimed in claim 1 or 2, which is characterized in that the training of the photosynthetic bacteria
The method of supporting is as follows:
A. 90~100 parts of distilled water, 30~35 parts of beef extract, 10~15 parts of glucose, 22~26 parts of sodium chloride, phosphorus are taken by weight
4~5 parts of sour hydrogen dipotassium, 4~5 parts of potassium dihydrogen phosphate, 3~5 parts of magnesium chloride, 0.1~0.3 part of N-methyl-N-nitrosourea, mixing
It stirs evenly, and sterilizing, obtains culture medium；
B. in 8~9 parts of photosynthetic bacteria strains of above-mentioned inoculation of medium, 2.5~3.5 parts of straw powders are added after inoculation, mixing is equal
It is even to be placed in constant incubator, it is 29~33 DEG C in temperature, speed cultivates 20~24 under conditions of being 110~130rpm
Hour；
C. after step b, 2~3 parts of starch is added, stir evenly, stand 40~50min, photosynthetic bacteria is made.
4. the implantation methods of environmental protection and public nuisance free rice as claimed in claim 3, which is characterized in that the photosynthetic bacteria strain is purple
The Mixed Microbes of color sulfur bacteria, cyanobacterium and green sulfur bacteria, and the purple sulfur bacteria, cyanobacterium and green sulfur bacteria are according to 1:
The ratio of 0.5~4:0.5~3 is mixed.
5. the implantation methods of environmental protection and public nuisance free rice as described in claim 3 or 4, which is characterized in that killing in the step a
The condition of bacterium disinfection: temperature is 115~126 DEG C, and the time is 15~25min.
6. the implantation methods of environmental protection and public nuisance free rice as described in claim 3 or 4, which is characterized in that stalk in the step b
Powder is stalk to pulverize and sieve for 80~150 mesh powders.
7. the implantation methods of environmental protection and public nuisance free rice as claimed in claim 5, which is characterized in that straw powder in the step b
To pulverize and sieve stalk for 80~150 mesh powders.
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| CN113303183B (en) * | 2021-06-10 | 2022-09-30 | 湖北师范大学 | Method for reducing total mercury in rice |
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