CN104045383A - Preparation method of compound microorganism fertilizer for planting nursery stock - Google Patents
Preparation method of compound microorganism fertilizer for planting nursery stock Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a preparation method of a compound microorganism fertilizer for planting a nursery stock. The method comprises the following steps: respectively activating photosynthetic bacteria, nitrogen-fixing bacteria, silicate bacteria, blue-green algae and saccharomyces cerevisiae in a frozen storage tube in a slope, and then purifying to obtain each cant strain for later use; preparing corresponding original bacterium fluid by using each cant strain; inoculating each original bacterium fluid into sterilized fermentation medium I according to the bacteria ratio of 2:2:2:2:1 to be cultivated, so as to obtain the first level of seeds of compound probiotics; inoculating the first level of seeds into the sterilized fermentation medium II at 5% of inoculation quantity to be cultivated, so as to obtain second level of seeds of the compound probiotics; finally inoculating the second level of seeds into the sterilized culture medium III at 5% of inoculation quantity to be cultivated, so as to obtain a compound probiotic finished product. By using the compound probiotics disclosed by the invention, not only can growth of the nursery stock be promoted, but also the survival rate of the planted and cultivated nursery stock can be improved. In addition, the compound probiotic also is ecological and environment-friendly, and low in cost.
Description
Technical field
The present invention relates to the compound micro-ecological preparation that a kind of plant cultivation is used, relate in particular to a kind of compound micro-ecological preparation for seedling growth.
Background technology
Along with the development of China's ecological construction, it is more and more important that afforestation seems in China's ecological construction, and people are to the quality of greening and require also more and more higher.And the plantation of nursery stock cultivation is the primary link of greening, in the plantation cultivating process of nursery stock, a large amount of nutrients of the growth needs of nursery stock are number of chemical element especially, existing is mainly that carbohydrate and the nutrient in soil of manufacturing by photosynthesis supplied the needs of its growth, but many times, nutrient content in photosynthesis and soil also cannot meet the demand of seedling growth, thereby causes seedling growth comparatively slow, and survival rate is also relatively low.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation method of the composite microbe fertilizer for seedling growth, utilizes its growth that not only can promote nursery stock, and can improve the surviving rate that seedling growth is cultivated.
The present invention solves the problems of the technologies described above by the following technical programs: a kind of compound micro-ecological preparation for seedling growth, and the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the photosynthetic bacteria in freezing pipe, vinelandii, silicate bacteria, blue-green algae, yeast saccharomyces cerevisiae are activated respectively in inclined-plane, be placed in respectively afterwards 90mm culture dish and carry out purifying, obtain photosynthetic bacteria slant strains, vinelandii slant strains, silicate bacteria slant strains, blue-green algae slant strains and yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: photosynthetic bacteria slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation and obtains photosynthetic bacteria original bacteria liquid for 5-7 days; Vinelandii slant strains is inoculated in sterilized liquid nutrient medium II, and then at 25 ℃-27 ℃, aerobic cultivation obtains vinelandii original bacteria liquid for 1-2 days; Silicate bacteria slant strains is inoculated in sterilized liquid nutrient medium III, and then at 28 ℃-30 ℃, aerobic cultivation obtains silicate bacteria original bacteria liquid for 1-2 days; Blue-green algae slant strains is inoculated in sterilized liquid nutrient medium IV, is then placed in 28 ℃-30 ℃ luminescent lamps and irradiates 10-14 hour and aerobic cultivation 3-5 days every day, obtain blue-green algae original bacteria liquid; Yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium V, and at 28 ℃-30 ℃, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid for 3 days afterwards;
(3) first order seed is cultivated: photosynthetic bacteria original bacteria liquid, vinelandii original bacteria liquid, silicate bacteria original bacteria liquid, blue-green algae original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid are inoculated in sterilized fermention medium I and are cultivated by the bacterium amount ratio of 2:2:2:2:1, particularly: first Azotobacter original bacteria liquid, silicate bacteria original bacteria liquid, blue-green algae original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and in 28 ℃ of-30 ℃ of aerobic cultivations 3 days, and then inoculation photosynthetic bacteria original bacteria liquid, in 37 ℃ of anaerobism, cultivate 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and at 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and at 28 ℃-30 ℃ first aerobic cultivations 3-5 days again anaerobism cultivate 3-5 days, the finished product of acquisition compound micro-ecological preparation.
Further, the concrete operations that in described step (1), photosynthetic bacteria activates in inclined-plane are: with transfering loop, photosynthetic bacteria is inoculated in sterilized slant medium I, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; Described oblique
The sterilising temp of face substratum I is that 121 ℃, sterilization time are 15min; And the component of this slant medium I: 10 grams of yeast powders, 1 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH7.0-7.2.
Further, the concrete operations that vinelandii activate in inclined-plane in described step (1) are: with transfering loop by inoculating in sterilized slant medium II, aerobic cultivation 1-2 days at 25 ℃-27 ℃ afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: 0.5 gram of yeast powder, 20 grams, N.F,USP MANNITOL, 0.2 gram of potassium primary phosphate, 0.8 gram of dipotassium hydrogen phosphate, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulfate, 15 grams, agar, 0.01 gram, iron(ic) chloride, 0.01 gram of Sodium orthomolybdate, 15 grams, agar, water 1000ml, PH7.2.
Further, the concrete operations that described step (1) mesosilicic acid salt bacterium activates in inclined-plane are: with transfering loop, silicate bacteria is inoculated in sterilized slant medium III, afterwards aerobic cultivation 1-2 days at 25 ℃-28 ℃; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; And the component of this slant medium III: 10 grams of sucrose, 5 grams, calcium sulfate, 0.2 gram of dipotassium hydrogen phosphate, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium-chlor, 14 grams, agar, water 1000ml, PH7.5.
Further, the concrete operations that in described step (1), blue-green algae activates in inclined-plane are: with transfering loop, blue-green algae is inoculated in sterilized slant medium IV, irradiates 10-14 hour and aerobic cultivation 3-5 days lower every day afterwards in 28 ℃-30 ℃ luminescent lamps; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 20min; And the component of this slant medium IV: 1.5 grams of SODIUMNITRATE, 0.05 gram of dipotassium hydrogen phosphate, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, 0.006 gram of citric acid, 0.006 gram of ironic citrate, 0.001 gram, zinc sulfate, 0.001 gram of Manganous chloride tetrahydrate, 20 grams, agar, water 1000ml, PH nature.
Further, the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated in sterilized slant medium V to aerobic cultivation 3 days at 28 ℃-30 ℃ afterwards; The sterilising temp of described slant medium V is that 121 ℃, sterilization time are 30min; And the component of this slant medium V: 200 grams of potatos, 20 grams of sucrose, agar 15-20 gram, water 1000ml, PH nature.
Further, the component of liquid nutrient medium I in described step (2): 10 grams of yeast powders, 1 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: 0.5 gram of yeast powder, 20 grams, N.F,USP MANNITOL, 0.8 gram of 0.2 gram of dipotassium hydrogen phosphate of potassium primary phosphate, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulfate,, 0.01 gram, iron(ic) chloride, 0.01 gram of Sodium orthomolybdate, water 1000ml, PH7.2; The component of liquid nutrient medium III: 10 grams of sucrose, 5 grams, calcium sulfate, 0.2 gram of dipotassium hydrogen phosphate, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium-chlor, water 1000ml, PH7.5; The component of liquid nutrient medium IV: 1.5 grams of SODIUMNITRATE, 0.05 gram of dipotassium hydrogen phosphate, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, 0.006 gram of citric acid, 0.006 gram of ironic citrate, 0.001 gram, zinc sulfate, 0.001 gram of Manganous chloride tetrahydrate, water 1000ml, PH nature; The component of liquid nutrient medium V: 200 grams of potatos, 20 grams of sucrose, agar 15-20 gram, water 1000ml, PH nature.
Further, the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
Further, the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
The preparation method's of a kind of composite microbe fertilizer for seedling growth of the present invention beneficial effect is: this compound micro-ecological preparation is sprayed on to plant and surrounding soil thereof, the growth of nursery stock not only can be promoted, and the surviving rate that seedling growth is cultivated can be improved; In addition, its also have advantages of ecological, environmental protective and cost low, the plantation that can be applicable on a large scale plant nursery stock is cultivated.
Embodiment
The preparation method of a kind of composite microbe fertilizer for seedling growth of the present invention, the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the photosynthetic bacteria in freezing pipe, vinelandii, silicate bacteria, blue-green algae, yeast saccharomyces cerevisiae are activated respectively in inclined-plane, be placed in respectively afterwards 90mm culture dish and carry out purifying, obtain photosynthetic bacteria slant strains, vinelandii slant strains, silicate bacteria slant strains, blue-green algae slant strains and yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: photosynthetic bacteria slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation and obtains photosynthetic bacteria original bacteria liquid for 5-7 days; Vinelandii slant strains is inoculated in sterilized liquid nutrient medium II, and then at 25 ℃-27 ℃, aerobic cultivation obtains vinelandii original bacteria liquid for 1-2 days; Silicate bacteria slant strains is inoculated in sterilized liquid nutrient medium III, and then at 28 ℃-30 ℃, aerobic cultivation obtains silicate bacteria original bacteria liquid for 1-2 days; Blue-green algae slant strains is inoculated in sterilized liquid nutrient medium IV, is then placed in 28 ℃-30 ℃ luminescent lamps and irradiates 10-14 hour and aerobic cultivation 3-5 days every day, obtain blue-green algae original bacteria liquid; Yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium V, and at 28 ℃-30 ℃, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid for 3 days afterwards;
(3) first order seed is cultivated: by the former bacterium of photosynthetic bacteria, vinelandii original bacteria liquid, silicate bacteria original bacteria liquid, blue-green algae original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid by 2: 2: 2: the bacterium amount ratio of 2: 1 is inoculated in sterilized fermention medium I to be cultivated, particularly: first Azotobacter original bacteria liquid, silicate bacteria original bacteria liquid, blue-green algae original bacteria liquid and yeast saccharomyces cerevisiae original bacteria liquid, and in 28 ℃ of-30 ℃ of aerobic cultivations 3 days, and then inoculation photosynthetic bacteria original bacteria liquid, in 37 ℃ of anaerobism, cultivate 3 days, obtain the first order seed of compound micro-ecological preparation;
(4) secondary seed is cultivated: the first order seed of gained is inoculated in sterilized fermention medium II by 5% inoculum size, and at 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and at 28 ℃-30 ℃ first aerobic cultivations 3-5 days again anaerobism cultivate 3-5 days, the finished product of acquisition compound micro-ecological preparation.
Wherein: the concrete operations that in step (1), photosynthetic bacteria activates in inclined-plane are: with transfering loop, photosynthetic bacteria is inoculated in sterilized slant medium I, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 15min; And the component of this slant medium I: 10 grams of yeast powders, 1 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH7.0-7.2.The concrete operations that vinelandii activate in inclined-plane in step (1) are: with transfering loop by inoculating in sterilized slant medium II, aerobic cultivation 1-2 days at 25 ℃-27 ℃ afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: 0.5 gram of yeast powder, 20 grams, N.F,USP MANNITOL, 0.2 gram of potassium primary phosphate, 0.8 gram of dipotassium hydrogen phosphate, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulfate, 15 grams, agar, 0.01 gram, iron(ic) chloride, 0.01 gram of Sodium orthomolybdate, 15 grams, agar, water 1000ml, PH7.2.The concrete operations that step (1) mesosilicic acid salt bacterium activates in inclined-plane are: with transfering loop, silicate bacteria is inoculated in sterilized slant medium III, afterwards aerobic cultivation 1-2 days at 25 ℃-28 ℃; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; And the component of this slant medium III: 10 grams of sucrose, 5 grams, calcium sulfate, 0.2 gram of dipotassium hydrogen phosphate, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium-chlor, 14 grams, agar, water 1000ml, PH7.5.The concrete operations that in step (1), blue-green algae activates in inclined-plane are: with transfering loop, blue-green algae is inoculated in sterilized slant medium IV, irradiates 10-14 hour and aerobic cultivation 3-5 days lower every day afterwards in 28 ℃-30 ℃ luminescent lamps; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 20min; And the component of this slant medium IV: 1.5 grams of SODIUMNITRATE, 0.05 gram of dipotassium hydrogen phosphate, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, 0.006 gram of citric acid, 0.006 gram of ironic citrate, 0.001 gram, zinc sulfate, 0.001 gram of Manganous chloride tetrahydrate, 20 grams, agar, water 1000ml, PH nature.The concrete operations that in step (1), yeast saccharomyces cerevisiae activates in inclined-plane are: with transfering loop, yeast saccharomyces cerevisiae is inoculated in to sterilized slant medium V
Upper, aerobic cultivation 3 days at 28 ℃-30 ℃ afterwards; The sterilising temp of described slant medium V is that 121 ℃, sterilization time are 30min; And the component of this slant medium V: 200 grams of potatos, 20 grams of sucrose, agar 15-20 gram, water 1000ml, PH nature.
The component of liquid nutrient medium I in step (2): 10 grams of yeast powders, 1 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: 0.5 gram of yeast powder, 20 grams, N.F,USP MANNITOL, 0.8 gram of 0.2 gram of dipotassium hydrogen phosphate of potassium primary phosphate, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulfate, 0.01 gram, iron(ic) chloride, 0.01 gram of Sodium orthomolybdate, water 1000ml, PH7.2; The component of liquid nutrient medium III: 10 grams of sucrose, 5 grams, calcium sulfate, 0.2 gram of dipotassium hydrogen phosphate, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium-chlor, water 1000ml, PH7.5; The component of liquid nutrient medium IV: 1.5 grams of SODIUMNITRATE, 0.05 gram of dipotassium hydrogen phosphate, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, 0.006 gram of citric acid, 0.006 gram of ironic citrate, 0.001 gram, zinc sulfate, 0.001 gram of Manganous chloride tetrahydrate, water 1000ml, PH nature; The component of liquid nutrient medium V: 200 grams of potatos, 20 grams of sucrose, agar 15-20 gram, water 1000ml, PH nature.
The component of fermention medium I in step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium II in step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.The component of fermention medium III in step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
It should be noted that, in the present invention, the per-cent of each substratum is mass percent.
Compound micro-ecological preparation of the present invention is for cultivating seedling growth, to promote the growth of nursery stock, and improve the surviving rate of nursery stock, applicant is to test as follows the purposes of explanation compound micro-ecological preparation of the present invention, particularly: get 20 strain qualities euscaphis konishii seedling unanimous on the whole, be divided at random two groups, one group is experimental group, and another group is control group; Test group adopts compound micro-ecological preparation of the present invention to process, control group adopts sterilized water to process: June 1 was transplanted to test group and control group in same environment, before transplanting, each euscaphis konishii root of test group is all immersed in to 5min in 100 times of diluents of compound micro-ecological preparation of the present invention, each euscaphis konishii root of control group is all immersed in 5min in sterilized water; After transplanting, 100 times of diluents of compound micro-ecological preparation of the present invention are evenly sprayed in test group plant and surrounding soil thereof by 0.5L/m2, control group sprays the sterilized water of equal parts, and experimental period is that June 1 was to October 10; Experimental result: test group is than control group, the growing height of nursery stock is higher than control group 12.75%, and surviving rate is higher than control group 20%, therefore, adopt compound micro-ecological preparation of the present invention not only can promote the growth of nursery stock, and can improve the surviving rate that seedling growth is cultivated.
To sum up, compound micro-ecological preparation of the present invention is sprayed in plant and surrounding soil thereof, not only can promotes the growth of nursery stock, and can improve the surviving rate that seedling growth is cultivated; In addition, this compound micro-ecological preparation also have advantages of ecological, environmental protective and cost low, the plantation that can be applicable on a large scale plant nursery stock is cultivated.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and in the situation that not deviating from spirit of the present invention or essential characteristic, can realize the present invention with other specific form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present invention is limited by claims rather than above-mentioned explanation, is therefore intended to include in the present invention dropping on the implication that is equal to important document of claim and all changes in scope.Any Reference numeral in claim should be considered as limiting related claim.
In addition, be to be understood that, although this specification sheets is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification sheets is only for clarity sake, those skilled in the art should make specification sheets as a whole, and the technical scheme in each embodiment also can, through appropriately combined, form other embodiments that it will be appreciated by those skilled in the art that.
Claims (6)
1. for a preparation method for the composite microbe fertilizer of seedling growth, it is characterized in that: the concrete steps of its preparation method are as follows:
(1) former strain inclined plane is cultivated: the photosynthetic bacteria in freezing pipe, vinelandii, silicate bacteria, blue-green algae, yeast saccharomyces cerevisiae are activated respectively in inclined-plane, be placed in respectively afterwards 90mm culture dish and carry out purifying, obtain photosynthetic bacteria slant strains, vinelandii slant strains, silicate bacteria slant strains, blue-green algae slant strains and yeast saccharomyces cerevisiae slant strains, standby;
(2) former bacterial classification liquid culture: photosynthetic bacteria slant strains is inoculated in sterilized liquid nutrient medium I, is placed in afterwards 28 ℃ of-30 ℃ of anaerobism tengsten lamp illumination cultivation and obtains photosynthetic bacteria original bacteria liquid for 5-7 days; Vinelandii slant strains is inoculated in sterilized liquid nutrient medium II, and then at 25 ℃-27 ℃, aerobic cultivation obtains vinelandii original bacteria liquid for 1-2 days; Silicate bacteria slant strains is inoculated in sterilized liquid nutrient medium III, and then at 28 ℃-30 ℃, aerobic cultivation obtains silicate bacteria original bacteria liquid for 1-2 days; Blue-green algae slant strains is inoculated in sterilized liquid nutrient medium IV, is then placed in 28 ℃-30 ℃ luminescent lamps and irradiates 10-14 hour and aerobic cultivation 3-5 days every day, obtain blue-green algae original bacteria liquid; Yeast saccharomyces cerevisiae slant strains is inoculated in sterilized liquid nutrient medium V, at 28 ℃-30 ℃, aerobic cultivation obtains yeast saccharomyces cerevisiae original bacteria liquid for 3 days afterwards, the component of liquid nutrient medium I in described step (2): 10 grams of yeast powders, 1 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, water 1000ml, PH7.0-7.2; The component of liquid nutrient medium II: 0.5 gram of yeast powder, 20 grams, N.F,USP MANNITOL, 0.8 gram of 0.2 gram of dipotassium hydrogen phosphate of potassium primary phosphate, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulfate, 0.01 gram, iron(ic) chloride, 0.01 gram of Sodium orthomolybdate, water 1000ml, PH7.2; The component of liquid nutrient medium III: 10 grams of sucrose, 5 grams, calcium sulfate, 0.2 gram of dipotassium hydrogen phosphate, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium-chlor, water 1000ml, PH7.5; The component of liquid nutrient medium IV: 1.5 grams of SODIUMNITRATE, 0.05 gram of dipotassium hydrogen phosphate, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, 0.006 gram of citric acid, 0.006 gram of ironic citrate, 0.001 gram, zinc sulfate, 0.001 gram of Manganous chloride tetrahydrate, water 1000ml, PH nature; The component of liquid nutrient medium V: 200 grams of potatos, 20 grams of sucrose, water 1000ml, PH nature;
(3) first order seed is cultivated: by the former bacterium of photosynthetic bacteria, vinelandii original bacteria liquid, silicate bacteria original bacteria liquid, blue-green algae original bacteria liquid, and yeast saccharomyces cerevisiae original bacteria liquid is inoculated in sterilized fermention medium I and cultivates by the bacterium amount ratio of 2:2:2:2:1, particularly: first Azotobacter original bacteria liquid, silicate bacteria original bacteria liquid, blue-green algae original bacteria liquid, and yeast saccharomyces cerevisiae original bacteria liquid, and in 28 ℃ of-30 ℃ of aerobic cultivations 3 days, and then inoculation photosynthetic bacteria original bacteria liquid, in 37 ℃ of anaerobism, cultivate 3 days, obtain the first order seed of compound micro-ecological preparation, the component of fermention medium I in described step (3): tangerine water 5%, yeast powder 0.5%, peptone 1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water,
(4) secondary seed is cultivated: the first order seed of gained is inoculated in to sterilized fermention medium II by 5% inoculum size
In, and at 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the secondary seed of compound micro-ecological preparation, the component of fermention medium II in described step (4): tangerine water 5%, yeast powder 0.1%, peptone 0.1%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water;
(5) finished product is cultivated: the secondary seed of gained is seeded in sterilized fermention medium III by 5% inoculum size, and at 28 ℃-30 ℃ first aerobic cultivations 3-5 days anaerobism cultivation 3-5 days again, obtain the finished product of compound micro-ecological preparation, the component of fermention medium III in described step (5): tangerine water 5%, ammonium chloride 0.2%, sodium-chlor 0.1%, potassium primary phosphate 0.1%, magnesium sulfate 0.05%, zinc sulfate 0.025%, ferrous sulfate 0.025%, surplus is water.
2. according to the preparation method of a kind of composite microbe fertilizer for seedling growth described in claim 1, it is characterized in that: the concrete operations that in described step (1), photosynthetic bacteria activates in inclined-plane are: with transfering loop, photosynthetic bacteria is inoculated in sterilized slant medium I, afterwards in 28 ℃-30 ℃ anaerobism tengsten lamp illumination cultivation 5-7 days; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 15min; And the component of this slant medium I: 10 grams of yeast powders, 1 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, 20 grams, agar, water 1000ml, PH7.0-7.2.
3. according to the preparation method of a kind of composite microbe fertilizer for seedling growth described in claim 1, it is characterized in that: the concrete operations that vinelandii activate in inclined-plane in described step (1) are: with transfering loop by inoculating in sterilized slant medium II, aerobic cultivation 1-2 days at 25 ℃-27 ℃ afterwards; The sterilising temp of described slant medium I is that 121 ℃, sterilization time are 20min; And the component of this slant medium I: 0.5 gram of yeast powder, 20 grams, N.F,USP MANNITOL, 0.2 gram of potassium primary phosphate, 0.8 gram of dipotassium hydrogen phosphate, 0.2 gram, magnesium sulfate, 0.1 gram, calcium sulfate, 15 grams, agar, 0.01 gram, iron(ic) chloride, 0.01 gram of Sodium orthomolybdate, 15 grams, agar, water 1000ml, PH7.2.
4. according to the preparation method of a kind of composite microbe fertilizer for seedling growth described in claim 1, it is characterized in that: the concrete operations that described step (1) mesosilicic acid salt bacterium activates in inclined-plane are: with transfering loop, silicate bacteria is inoculated in sterilized slant medium III, afterwards aerobic cultivation 1-2 days at 25 ℃-28 ℃; The sterilising temp of described slant medium III is that 121 ℃, sterilization time are 20min; And the component of this slant medium III: 10 grams of sucrose, 5 grams, calcium sulfate, 0.2 gram of dipotassium hydrogen phosphate, 5 grams, calcium carbonate, 0.2 gram, magnesium sulfate, 0.2 gram, sodium-chlor, 14 grams, agar, water 1000ml, PH7.5.
5. according to the preparation method of a kind of composite microbe fertilizer for seedling growth described in claim 1, it is characterized in that: the concrete operations that in described step (1), blue-green algae activates in inclined-plane are: with transfering loop, blue-green algae is inoculated in sterilized slant medium IV, irradiates 10-14 hour and aerobic cultivation 3-5 days lower every day afterwards in 28 ℃-30 ℃ luminescent lamps; The sterilising temp of described slant medium IV is that 121 ℃, sterilization time are 20min; And the component of this slant medium IV: 1.5 grams of SODIUMNITRATE, 0.05 gram of dipotassium hydrogen phosphate, 0.08 gram, magnesium sulfate, 0.04 gram, calcium chloride, 0.02 gram, sodium carbonate, 0.006 gram of citric acid, 0.006 gram of ironic citrate, 0.001 gram, zinc sulfate, 0.001 gram of Manganous chloride tetrahydrate, 20 grams, agar, water 1000ml, PH nature.
6. according to the preparation method of a kind of composite microbe fertilizer for seedling growth described in claim 1, it is characterized in that: the concrete operations that yeast saccharomyces cerevisiae activates in inclined-plane in described step (1) are: with transfering loop, yeast saccharomyces cerevisiae is inoculated in sterilized slant medium V to aerobic cultivation 3 days at 28 ℃-30 ℃ afterwards; The sterilising temp of described slant medium V is that 121 ℃, sterilization time are 30min; And the component of this slant medium V: 200 grams of potatos, 20 grams of sucrose, agar 15-20 gram, water 1000ml, PH nature.
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| CN109089807A (en) * | 2018-08-30 | 2018-12-28 | 新疆光合元生物科技有限公司 | A kind of implantation methods of environmental protection and public nuisance free rice |
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