CN109043538A - A kind of preparation method of okra composition - Google Patents
A kind of preparation method of okra composition Download PDFInfo
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- CN109043538A CN109043538A CN201810942225.7A CN201810942225A CN109043538A CN 109043538 A CN109043538 A CN 109043538A CN 201810942225 A CN201810942225 A CN 201810942225A CN 109043538 A CN109043538 A CN 109043538A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 20
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- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 12
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of preparation methods of okra composition, belong to Biochemical Engineering technical field, the preparation method of the okra composition is that okra composition then is made by certain formulation ratio wet granulation, tabletting first by producing okra water extract.Okra water extract made from this method and okra composition, can be improved blood sugar concentration in animal movement, be conducive to reinforce to load performance；More energy can be provided for body by increasing liver glycogen reserves；Have the function of improving body aerobic capacity；There is the effect of good anti-sports type fatigue.
Description
Technical field
The present invention relates to Biochemical Engineering technical fields, more particularly relate to the preparation method of okra composition.
Background technique
Okra is Malvaceae Abelmoschus annual herb plant also known as woman refers to, kidney tonifying grass, coffee certain herbaceous plants with big flowers etc..Gumbo is former
African torrid areas is originated in, is now distributed widely in all over the world.The fresh okra fruit of okra can be edible, wherein rich in
The various bioactive components such as protein, unsaturated fatty acid, free amino acid, minerals, vitamin, alkaloid are both battalion
Delicious vegetables abundant are supported, and have medicinal health effect.In addition, mucilaginous substance also rich in its fruit, has and promotes stomach
The effect of enterocinesia, gastritis, defaecation.Okra is lightly seasoned, cold in nature, have relieving sore-throat, treating stranguria, lower cream, menstruation regulating and other effects, leaf,
Bud, flower are also edible, and flower, seed, root can be used as medicine.Seed fries grind after, have the fragrance of coffee, but decaffeinated,
Therefore it can be used as the substitute of coffee.Okra nutriment rich in, the edible self-care that human body can be promoted comprehensive,
To have it is hypoglycemic, by blood lipid, anti-aging, it is antifatigue, improve immunity, enhancing human body anti-cancer, anti-cancer ability and other effects.
Fatigue is defined as the body or mental exertion of extreme.It is a kind of physiological phenomenon of complexity, may be along with
Many diseases and occur.Chinese medicine is to the understanding of sports fatigue, from entirety, it is believed that fatigue essence mainly with spleen, the basic machine of kidney
It can change or the extent of damage is closely related.Currently, making internal disorder or usurp for antifatigue grinding, domestic literature report is more, and external related text
It is less to offer report.Oneself the antifatigue substance of report is all the active constituent extracted from Chinese medicine and natural products mostly, and is concentrated
The substance necessary to the body metabolisms such as energy matter and microelement.Oneself has document confirmation, and okra fruit pod powder and its water mention
Liquid has the effect of good anti-mouse sports type fatigue, but does not illustrate the substance specifically to work and specific effect machine
Reason.
Increasingly increase with society to functional food demand, okra is because of its special functional characteristic and good
Medical value is more and more widely paid attention to.Due to its nutriment rich in, high-grade vegetables were not only can be used as, but also can
To develop into novel functional food and drug.
Summary of the invention
In order to overcome the drawbacks of the prior art, technical problem to be solved by the present invention lies in propose a kind of okra combination
The preparation method of object.
For this purpose, preparation process of the invention the following steps are included:
The present invention provides a kind of preparation methods of okra composition, implement in accordance with the following steps:
S1: okra raw material being mixed with water, solid-liquid ratio 1g:15-30ml, 0.3-2h is impregnated, in 70-100 DEG C of temperature
Lower extraction 1-3h, after separation of solid and liquid extracting solution I and solid phase I；
The extracting mode is carried out using one of ultrasonic extraction, Microwave Extraction, refluxing extraction mode；
S2: solid phase I obtained by step 1) is mixed with water, and solid-liquid ratio 1g:10-20ml after extracting 0.5-1h, is separated by solid-liquid separation
Extracting solution II is obtained afterwards；
S3: concentrate is obtained after the mixed liquor of extracting solution I or extracting solution I and extracting solution II is concentrated under reduced pressure；
S4: concentrate is dry using any one mode in heated-air drying, freeze-drying, vacuum drying, ultrasonic wave drying
It is dry to get okra water extract；
S5: taking 120-160 parts of okra water extract by weight, and 20-50 parts of rhizoma polygonati water extract, Fructus lycii P.E 20-
50 parts；10-20 parts of pharmaceutic adjuvant, the ethyl alcohol that volumetric concentration is 80-95% is then added, carries out wet granulation, tabletting to get Huang
Gumbo composition；
The Extraction solvent of the rhizoma polygonati water extract is not less than 10 times of raw material；Polyoses content is not in the Fructus lycii P.E
Lower than 20wt%；The pharmaceutic adjuvant include dextrin, starch, sucrose, microcrystalline cellulose, mannitol, lactose, pregelatinized starch,
At least one of magnesium stearate.
In preferably technical solution of the invention, in the S1 step, okra raw material is mixed with water, solid-liquid ratio is
1g:20-30ml impregnates 1-2h, extracts 1.5-2h, obtains extracting solution I and solid phase I after separation of solid and liquid.
In preferably technical solution of the invention, the extracting mode uses the ultrasonic power of ultrasonic wave extraction for 100-
200W/L；Extraction time 1-3h.
In preferably technical solution of the invention, the extracting mode uses the power of Microwave Extraction for 200-500W/
L；Extraction time 2-5h.
In preferably technical solution of the invention, in the S2 step, solid phase I obtained by step S1 is mixed with water, feed liquid
Than for 1g:10-15ml, 80-90 DEG C at a temperature of extract 0.5-1h after, after separation of solid and liquid extracting solution II.
In preferably technical solution of the invention, in the S4 step, drying mode uses heated-air drying, and heating temperature is
40-70 DEG C, preferably 55~65 DEG C, more preferable 60 DEG C.
It in preferably technical solution of the invention, states in S4 step, drying mode is using vacuum drying, heating temperature
40-60 DEG C, preferably 45~55 DEG C, more preferable 50 DEG C.
In preferably technical solution of the invention, in the S4 step, drying mode is dry using ultrasonic wave, ultrasonic wave function
Rate is 10-100W/cm2, drying temperature is 20-30 DEG C.
In preferably technical solution of the invention, in the S5 step, okra water extract 130-150 is taken by weight
Part, more preferable 140 parts, 20-40 parts of rhizoma polygonati water extract, more preferable 30 parts, 20-40 parts of Fructus lycii P.E, more preferable 30 parts；Medicine
With 15 parts of auxiliary material.
In preferably technical solution of the invention, in the S5 step, the ethyl alcohol that volumetric concentration is 90-95% is added, into
Row wet granulation.
The invention has the benefit that
The preparation method of okra composition provided by the invention, okra water extract and okra combination obtained
Object can be improved the concentration of blood glucose in animal movement, be conducive to reinforce to load performance；It can be by increasing liver glycogen reserves
More energy are provided for body；Have the function of improving body aerobic capacity；There is the effect of good anti-sports type fatigue
Fruit.
Detailed description of the invention
Fig. 1 a is changes of weight before and after all animal feedings that the specific embodiment of the invention provides；
Fig. 1 b is changes of weight before and after the swimming with a load attached to the body animal feeding that the specific embodiment of the invention provides；
Fig. 2 is the experimental animal walking weight load that the specific embodiment of the invention provides；
Fig. 3 is experimental animal serum urea level after the movement that the specific embodiment of the invention provides；
Fig. 4 is each group hepatic glycogen content after the administration that the specific embodiment of the invention provides；
Fig. 5 is each dosage group blood lactase acid level of experimental animal before and after the movement that the specific embodiment of the invention provides.
Specific embodiment
To further illustrate the technical scheme of the present invention below with reference to the accompanying drawings and specific embodiments.
Embodiment 1 prepares okra water extract 1#And composition A1
Okra dry plate 100g is weighed, 20 times of amount (2L) distilled water are added, impregnates 20min, 85 DEG C of refluxing extraction 2h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, and gained solid phase I is mixed with water, and solid-liquid ratio 1:13 is mentioned at 85 DEG C
1h is taken, extracting solution II is obtained after separation of solid and liquid, extracting solution I and the mixed liquor of extracting solution II is concentrated under reduced pressure, 65 DEG C of hot air dryings
It is dry, obtain okra water extract 32.5g, water extract yield is that 32.5% (wherein polysaccharide yield is 5.2%, flavones yield is
0.57%), it is denoted as 1#.
Weigh 1#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.2g with 12 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 90vol.%, press
Piece obtains composition A1.
Wherein, the pharmaceutic adjuvant is dextrin.
Embodiment 2 prepares okra water extract 2#And composition A2
Okra dry plate 100g is weighed, 25 times of amount (2.5L) distilled water are added, impregnates 20min, 30 DEG C of 150W/L ultrasounds mention
Time 1h is taken, is filtered, is centrifuged, extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 65 DEG C of hot winds
It is dry, obtain okra water extract 31.1g, water extract yield is that 33.1% (wherein polysaccharide yield is 5.3%, flavones yield is
0.59%), it is denoted as 2#.
Weigh 2#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.5g with 15 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, press
Piece obtains composition A2.
Wherein, the pharmaceutic adjuvant is starch.
Embodiment 3 prepares okra water extract 3#And composition A3
Okra dry plate 100g is weighed, 20 times of amount (2L) distilled water are added, impregnates 20min, 300W/L Microwave Extraction 2h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 60 DEG C of heated-air dryings obtain Huang
Gumbo water extract 29.5g, water extract yield are 29.5% (wherein polysaccharide yield is 5.4%, flavones yield is 0.60%), are denoted as
3#。
Weigh 3#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.0g with 10 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 90vol.%, press
Piece obtains composition A3.
Wherein, the pharmaceutic adjuvant is microcrystalline cellulose.
Embodiment 4 prepares okra water extract 4#And composition A4
Okra dry plate 100g is weighed, 30 times of amount (3L) distilled water are added, impregnates 20min, 90 DEG C of refluxing extraction 2h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, and gained solid phase I is mixed with water, and solid-liquid ratio 1:10 is mentioned at 80 DEG C
1h is taken, extracting solution II is obtained after separation of solid and liquid, extracting solution I and the mixed liquor of extracting solution II is freeze-dried, okra is obtained
Water extract 34.8g, water extract yield are 34.8% (wherein polysaccharide yield is 5.9%, flavones yield is 0.51%), are denoted as 4#.
Weigh 4#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.4g with 14 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, press
Piece obtains composition A4.
Wherein, the pharmaceutic adjuvant is mannitol.
Embodiment 5 prepares okra water extract 5#And composition A5
Okra dry plate 100g is weighed, 20 times of amount (2L) distilled water are added, impregnates 20min, 85 DEG C of refluxing extraction 1.5h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, and 50 DEG C of vacuum drying obtain Huang
Gumbo water extract 30.7g, water extract yield are 30.7% (wherein polysaccharide yield is 5.4%, flavones yield is 0.56%), are denoted as
5#。
Weigh 5#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.2g with 12 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 90vol.%, press
Piece obtains composition A5.
Wherein, the pharmaceutic adjuvant is lactose.
Embodiment 6 prepares okra water extract 6#And composition A6
Okra dry plate 100g is weighed, 15 times of amount (1.5L) distilled water are added, impregnates 20min, 40 DEG C of 150W/L ultrasounds mention
Time 1.5h is taken, obtains extracting solution I and solid phase I after separation of solid and liquid, gained solid phase I is mixed with water, solid-liquid ratio 1:15, at 90 DEG C
0.8h is extracted, extracting solution II is obtained after separation of solid and liquid, extracting solution I and the mixed liquor of extracting solution II is concentrated under reduced pressure, 65 DEG C of heat
It air-dries dry, obtains okra water extract 32.6g, water extract yield is that 32.6% (wherein polysaccharide yield is 4.9%, flavones yield
0.55%), to be denoted as 6#.
Weigh 6#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.5g with 15 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 90vol.%, press
Piece obtains composition A6.
Wherein, the pharmaceutic adjuvant is pregelatinized starch.
Embodiment 7 prepares okra water extract 7#And composition A7
Okra dry plate 100g is weighed, 25 times of amount (2.5L) distilled water are added, impregnates 20min, 70 DEG C of refluxing extraction 3h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 60 DEG C of heated-air dryings obtain Huang
Gumbo water extract 30.1g, water extract yield are 30.1% (wherein polysaccharide yield is 4.8%, flavones yield is 0.53%), are denoted as
7#。
Weigh 7#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.4g with 14 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, press
Piece obtains composition A7.
Wherein, the pharmaceutic adjuvant is that dextrin and starch are formed according to 1:1 proportion.
Embodiment 8 prepares okra water extract 8#And composition A8
Okra dry plate 100g is weighed, 20 times of amount (2L) distilled water are added, impregnates 20min, 90 DEG C of refluxing extraction 1h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, and is freeze-dried, is obtained okra
Water extract 33.1g, water extract yield are 33.1% (wherein polysaccharide yield is 5.8%, flavones yield is 0.51%), are denoted as 8#.
Weigh 8#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.5g with 15 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, press
Piece obtains composition A8.
Wherein, the pharmaceutic adjuvant is that magnesium stearate and pregelatinized starch are formed according to 1:1 proportion.
Embodiment 9 prepares okra water extract 9#And composition A9
Okra dry plate 100g is weighed, 15 times of amount (1.5L) distilled water are added, impregnates 20min, 70 DEG C of refluxing extraction 2h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, and is freeze-dried, is obtained okra
Water extract 28.8g, water extract yield are 28.8% (wherein polysaccharide yield is 4.6%, flavones yield is 0.55%), are denoted as 9#.
Weigh 9#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 41.3g with 13 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 90vol.%, press
Piece obtains composition A9.
Wherein, the pharmaceutic adjuvant is that sucrose and microcrystalline cellulose are formed according to 1:1 proportion.
Embodiment 10 prepares okra water extract 10#And composition A10
Okra dry plate 1.8Kg is weighed, 20 times of amount (36L) distilled water are added, impregnates 1h, 85 DEG C of refluxing extraction 2h, is filtered,
It is centrifuged, extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 65 DEG C of heated-air dryings obtain okra
Water extract 549g, water extract yield are 30.5% (wherein polysaccharide yield is 5.4%, flavones yield is 0.56%), are denoted as 10#.
Weigh 10#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 636g with 12 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 90vol.%, tabletting,
Obtain composition A10.
Wherein, the pharmaceutic adjuvant is that mannitol and lactose are formed according to 1:1 proportion.
Embodiment 11 prepares okra water extract 11#And composition A11
Okra dry plate 1.8g is weighed, 25 times of amount (45L) distilled water are added, impregnates 1h, 80 DEG C of refluxing extraction 2h, is filtered,
It is centrifuged, extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 20 DEG C, 50W/cm2 ultrasound is dry, obtains
To okra water extract 535g, water extract yield is 29.7% (wherein polysaccharide yield is 5.7%, flavones yield is 0.58%),
It is denoted as 11#.
Weigh 11#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 642g with 14 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, tabletting,
Obtain composition A11.
Wherein, the pharmaceutic adjuvant is that magnesium stearate and microcrystalline cellulose are formed according to 1:1 proportion.
Embodiment 12 prepares okra water extract 12#And composition A12
Okra dry plate 2.0kg is weighed, 20 times of amount (40L) distilled water are added, impregnates 2h, 85 DEG C of refluxing extraction 2h, is filtered,
It is centrifuged, extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 65 DEG C of heated-air dryings obtain okra
Water extract 646g, water extract yield are 32.3% (wherein polysaccharide yield is 5.9%, flavones yield is 0.56%), are denoted as 12#.
Weigh 12#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 848g with 12 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, tabletting,
Obtain composition A12.
Wherein, the pharmaceutic adjuvant is that mannitol and pregelatinized starch are formed according to 1:1 proportion.
Embodiment 13 prepares okra water extract 13#And composition A13
Okra dry plate 2.5kg is weighed, 20 times of amount (50L) distilled water are added, impregnates 1.5h, 85 DEG C of refluxing extraction 2h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 65 DEG C of heated-air dryings obtain Huang
Gumbo water extract 780g, water extract yield are 31.2% (wherein polysaccharide yield is 5.2%, flavones yield is 0.57%), are denoted as
13#。
Weigh 13#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 1060g with 12 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, press
Piece obtains composition A13.
Wherein, the pharmaceutic adjuvant is that lactose, magnesium stearate and pregelatinized starch are formed according to 1:1:1 proportion.
Embodiment 14 prepares okra water extract 14#And composition A14
Okra dry plate 3.6g is weighed, 20 times of amount (72L) distilled water are added, impregnates 0.5h, 85 DEG C of refluxing extraction 2h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 60 DEG C of heated-air dryings obtain Huang
Gumbo water extract 1090g, water extract yield are 30.3% (wherein polysaccharide yield is 5.2%, flavones yield is 0.57%), are denoted as
14#。
Weigh 14#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 212g with 12 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 90vol.%, tabletting,
Obtain composition A14.
Wherein, the pharmaceutic adjuvant is that microcrystalline cellulose, magnesium stearate and pregelatinized starch are formed according to 1:1:1 proportion.
Embodiment 15 prepares okra water extract 15#And composition A15
Okra dry plate 90kg is weighed, 20 times of amount (1800L) distilled water are added, impregnates 2h, 70 DEG C of refluxing extraction 3h, mistake
Filter is centrifuged, and extracting solution I and solid phase I is obtained after separation of solid and liquid, extracting solution I is concentrated under reduced pressure, 60 DEG C of heated-air dryings obtain Huang
Gumbo water extract 26.43kg, water extract yield are 29.4% (wherein polysaccharide yield is 5.2%, flavones yield is 0.57%), note
For 15#.
Weigh 15#140 parts of okra water extract in parts by weight, 30 parts of rhizoma polygonati water extract, 30 parts of Fructus lycii P.E, medicine
It is total to obtain mixture 21.2kg with 12 parts of auxiliary material, it is uniformly mixed, uses concentration for the ethyl alcohol wet granulation of 95vol.%, press
Piece obtains composition A15.
Wherein, the pharmaceutic adjuvant is mannitol, microcrystalline cellulose, magnesium stearate and pregelatinized starch according to 1:1:1:1
Proportion composition.
14# okra water extract prepared by the present invention alleviates having the beneficial effect that for physical fatigue:
(1) experimental animal: SPF grades of CD-1 (ICR) mouse are purchased from Beijing Vital River Experimental Animals Technology Co., Ltd., altogether
160, male, 27-32g, credit number SCXK (capital) 2016-0011, Quality of Experimental Animals quality certification number
No.11400700229272.Mouse is raised in barrier environment IVC cage box, and 5/cage, 12 hours light and shades of animal house illumination are handed over
It replaces, 22-26 DEG C of temperature, humidity 40-60%.Mouse free water is ingested in experimentation.Experimental animal operation and processing are deferred to
Experimental animal Ethic review guidelines.
(2) experimental group and drug treatment scheme
160 male ICR mouses, weight 27-32g are random according to weight in animal house adaptive feeding 1 week after buying
Be divided into 4 groups, be respectively as follows: Normal group, YP-1 it is low, in and high dose group.Mice group and drug treatment scheme see the table below.
Mice group and dosage regimen (d1-d30)
1. weight records
Judge that high, medium and low dosage group drug is to experimental animal weight from experimental animal weight incrementss all during stomach-filling
Influence low (Fig. 1 a).In the animal of swimming with a load attached to the body Indexs measure, high dose group the weight of animals and control group significant difference (figure
1b), infer that the gastric infusion of high dose there may be certain side effect to experimental animal.The groups of animals of other each index animals
Weight indifference exclusive or difference between control group is not significant.
2. walking weight load
Loaned swimming test is the most direct-vision method for detecting drug fatigue effect.The result shows that middle dose group extend it is small
Mouse walking weight load has reached significance (P < 0.05).Compared with the control group and indifference, this may be low dose group
Caused by underdosage.The high dose group mice burden swimming time is reduced compared with control group, but not up to significance (P >
0.05).This may be that high dose group gives that sample is more, the reason (table 1, Fig. 2) of the discomfort of mouse caused by stomach-filling.
1 experimental animal walking weight load of table
3. serum urea content
Energy needed for animal movement is mainly provided by glucose, when blood glucose insufficient supply, protein degradation offer portion
Divide energy.And the nitrogen of breaks down proteins is recycled by ornithine and generates urea, causes the raising of blood urea nitrogen.Table 2 and Fig. 3 number
According to show sample can inhibit movement after mouse urea nitrogen concentration increase, three dosage groups reached significance (P <
0.05), illustrate that it can be improved the concentration of blood glucose in animal movement, be conducive to reinforce to relieve fatigue load performance.
2 experimental animal serum urea of table is horizontal
4. hepatic glycogen measures
Glycogen is the main energy sources of strenuous exercise, it can be in a manner of anerobic glycolysis but also with aerobic metabolism side
Formula synthesizes ATP again.Hepatic glycogen and muscle glycogen are energy reserve and the direct tissue utilized respectively, and it is movement energy supply that oxidation, which utilizes,
Main source.The failure of body function always exhausts while occurring with glycogen, therefore the content of glycogen can illustrate tired hair
Raw speed or degree.If tested material group hepatic glycogen is apparently higher than control group, and difference is statistically significant, then explanation should be by
Examination object can provide more energy by increasing liver glycogen reserves for body, achieve the purpose that antifatigue.
After administration 30 days, liver glycogen reserves are can be improved in pharmaceutical intervention group, wherein low, high dose group difference highly significant (p <
0.01) (table 3, Fig. 4)
3 experimental animal hepatic glycogen content of table
5. blood lactase acid measures
Lactic acid is glycogen anaerobic metabolism product, in fierce power or static exercise, is increased about in lactic acid in muscle
30 times, lactic acid is easy to accumulate, due to lactic acid in muscle bulk deposition, increase muscle middle acid substance, and lead to fatigue.If
Oxygen is sufficient in blood, and lactic acid just further decomposes into CO2 and water；If oxygen is insufficient in blood, lactic acid concn is increased in blood, is just drawn
Play muscular fatigue.Table 4 and Fig. 5 show that animal pattern gives middle dosage and the drug of high dose is fed, and lactic acid can after vigorous exercise
Fast quick-recovery (decline is obvious, is in dose dependent), serum K+ is significantly better than control group (p < 0.05), illustrates sample
Have the function of improving body aerobic capacity, so that mice burden swimming is restored to normal level after stopping 30min, postpone
The appearance of fatigue.
4 experimental animal blood lactase acid of table is horizontal
Referring to " health food is examined and assessment technique specification " result judgement guide: swimming with a load attached to the body experimental result is positive, blood
Lactic acid, serum urea, wantonly two index is positive in three indexs of hepatic glycogen/muscle glycogen, can determine whether that the given the test agent has and alleviates body
The effect of power fatigue function.Sample anti-fatigue effect evaluation result shows that middle dose group can significantly extend the mice burden swimming time
(p < 0.01), middle dose group serum urea level declines significant (p < 0.05) compared to control group, while middle dose group can be significant
It improves serum K+ (p < 0.05).In addition, the serum urea (p < 0.05) of high dose group, hepatic glycogen (p < 0.01), blood
There were significant differences with control group for three indexs of lactic acid (p < 0.05), serum urea (p < 0.001), the hepatic glycogen (p of low dose group
< 0.0001) also there were significant differences with control group for index.In conclusion " health food is examined advises samples met with assessment technique
Model " in antifatigue criterion, judgement have the function of relieving fatigue.
The present invention is described with reference to the preferred embodiments, and those skilled in the art know, of the invention not departing from
In the case where spirit and scope, various changes or equivalence replacement can be carried out to these features and embodiment.The present invention is not by this
The limitation of specific embodiment disclosed in place, other embodiments fallen into claims hereof belong to protection of the present invention
Range.
Claims (10)
1. a kind of preparation method of okra composition, which is characterized in that preparation process the following steps are included:
S1: okra raw material being mixed with water, solid-liquid ratio 1g:15-30ml, is impregnated 0.3-2h, is extracted 1- at 70-100 DEG C
3h, after separation of solid and liquid extracting solution I and solid phase I；
The extracting mode is carried out using one of ultrasonic extraction, Microwave Extraction, refluxing extraction mode；
S2: solid phase I obtained by step S1 being mixed with water, solid-liquid ratio 1g:10-20ml, 70-100 DEG C at a temperature of extract
After 0.5-1h, extracting solution II is obtained after separation of solid and liquid；
S3: concentrate is obtained after the mixed liquor of extracting solution I or extracting solution I and extracting solution II is concentrated under reduced pressure；
S4: concentrate is dry using any one mode in heated-air drying, freeze-drying, vacuum drying, ultrasonic wave drying, i.e.,
Obtain okra water extract；
S5: 120-160 parts of okra water extract, 20-50 parts of rhizoma polygonati water extract, 20-50 parts of Fructus lycii P.E are taken by weight；
10-20 parts of pharmaceutic adjuvant, the ethyl alcohol that volumetric concentration is 80-95% is then added, carries out wet granulation, tabletting to get okra
Composition；
The Extraction solvent of the rhizoma polygonati water extract is not less than 10 times of raw material；Polyoses content is not less than in the Fructus lycii P.E
20wt%；The pharmaceutic adjuvant includes dextrin, starch, sucrose, microcrystalline cellulose, mannitol, lactose, pregelatinized starch, tristearin
At least one of sour magnesium.
2. the preparation method of okra composition according to claim 1, it is characterised in that:
In the S1 step, okra raw material is mixed, solid-liquid ratio 1g:20-30ml with water, impregnates 1-2h, extracts 1.5-2h,
Extracting solution I and solid phase I is obtained after separation of solid and liquid.
3. the preparation method of okra composition according to claim 1, it is characterised in that: the extracting mode uses
The ultrasonic power of ultrasonic wave extraction is 100-200W/L；Extraction time 1-3h.
4. the preparation method of okra composition according to claim 1, it is characterised in that: the extracting mode uses
The power of Microwave Extraction is 200-500W/L；Extraction time 2-5h.
5. the preparation method of okra composition according to claim 1, it is characterised in that: in the S2 step, will walk
Solid phase I obtained by rapid S1 mix with water, solid-liquid ratio 1g:10-15ml, 80-90 DEG C at a temperature of extraction 0.5-1h after, solid-liquid divides
Extracting solution II is obtained from after.
6. the preparation method of okra composition according to claim 1, it is characterised in that: dry in the S4 step
Mode uses heated-air drying, and heating temperature is 40-70 DEG C, preferably 55~65 DEG C, more preferable 60 DEG C.
7. the preparation method of okra composition according to claim 1, it is characterised in that: dry in the S4 step
For mode using vacuum drying, heating temperature is 40-60 DEG C, preferably 45~55 DEG C, more preferable 50 DEG C.
8. the preparation method of okra composition according to claim 1, it is characterised in that: dry in the S4 step
Mode is dry using ultrasonic wave, ultrasonic power 10-100W/cm2, drying temperature is 20-30 DEG C.
9. the preparation method of okra composition according to claim 1, it is characterised in that: in the S5 step, by weight
Amount part takes 130-150 parts of okra water extract, and more preferable 140 parts, 20-40 parts of rhizoma polygonati water extract, more preferable 30 parts, fructus lycii mentions
Take 20-40 parts of object, more preferable 30 parts；15 parts of pharmaceutic adjuvant.
10. the preparation method of okra composition according to claim 1, it is characterised in that: in the S5 step, be added
Volumetric concentration is the ethyl alcohol of 90-95%, carries out wet granulation.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797274A (en) * | 2010-04-09 | 2010-08-11 | 三门富达果蔬专业合作社 | Preparation technology of okra capsule |
| CN104287047A (en) * | 2014-10-21 | 2015-01-21 | 浙江葵瑞农业开发有限公司 | Production process of Abelmoschus esculentus solid drink |
| CN104366467A (en) * | 2014-10-21 | 2015-02-25 | 方道会 | Production process of tablets with okra |
| CN106820140A (en) * | 2017-01-03 | 2017-06-13 | 河北省农林科学院经济作物研究所 | A kind of functional food for alleviating exercise induced fatigue |
| CN107998218A (en) * | 2017-12-29 | 2018-05-08 | 内蒙古阿拉善苁蓉集团有限责任公司 | A kind of composition and preparation method thereof and pharmacy application |
-
2018
- 2018-08-17 CN CN201810942225.7A patent/CN109043538A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797274A (en) * | 2010-04-09 | 2010-08-11 | 三门富达果蔬专业合作社 | Preparation technology of okra capsule |
| CN104287047A (en) * | 2014-10-21 | 2015-01-21 | 浙江葵瑞农业开发有限公司 | Production process of Abelmoschus esculentus solid drink |
| CN104366467A (en) * | 2014-10-21 | 2015-02-25 | 方道会 | Production process of tablets with okra |
| CN106820140A (en) * | 2017-01-03 | 2017-06-13 | 河北省农林科学院经济作物研究所 | A kind of functional food for alleviating exercise induced fatigue |
| CN107998218A (en) * | 2017-12-29 | 2018-05-08 | 内蒙古阿拉善苁蓉集团有限责任公司 | A kind of composition and preparation method thereof and pharmacy application |
Non-Patent Citations (2)
| Title |
|---|
| 国家药典委员会: "《中华人民共和国药典临床用药须知 中药饮片卷 2015年版》", 31 October 2017, 中国医药科技出版社 * |
| 钟雨等: "黄秋葵水提物抗运动疲劳作用研究", 《食品科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601684A (en) * | 2019-01-22 | 2019-04-12 | 广州市顺芝堂生物科技有限公司 | A kind of gumbo mulberry leaf pressed candy and preparation method thereof |
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