CN109042676A - A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol - Google Patents

A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol Download PDF

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Publication number
CN109042676A
CN109042676A CN201810926193.1A CN201810926193A CN109042676A CN 109042676 A CN109042676 A CN 109042676A CN 201810926193 A CN201810926193 A CN 201810926193A CN 109042676 A CN109042676 A CN 109042676A
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CN
China
Prior art keywords
azoles bacterium
acyl azanol
fluorine azoles
bacterium acyl
agent
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Pending
Application number
CN201810926193.1A
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Chinese (zh)
Inventor
葛家成
李健
刘桂娟
杨志鹏
巨翠红
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Hailir Pesticides and Chemicals Group Co Ltd
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Hailir Pesticides and Chemicals Group Co Ltd
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Priority to CN201810926193.1A priority Critical patent/CN109042676A/en
Publication of CN109042676A publication Critical patent/CN109042676A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/32—Cyclic imides of polybasic carboxylic acids or thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/88—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with three ring hetero atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof

Abstract

The present invention provides a kind of bactericidal compositions of fluorine-containing azoles bacterium acyl azanol, it is characterized in that other than component A fluorine azoles bacterium acyl azanol, it further include at least one other fungicide as B component medicament, and the auxiliary agent that can be used in Pesticide formulation, conventional pesticidal preparations are made, and are applied to controlling plant diseases.

Description

A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol
Technical field:
The invention belongs to pesticides application technical fields, are related to a kind of composition pesticide, specifically a kind of to contain fluorine azoles The bactericidal composition of bacterium acyl azanol is applied to controlling plant diseases.
Background technique:
In agricultural production process, plant disease occurs seriously, to endanger the positions such as plant roots, stem, leaf and fruit, kind The organs of multiplication such as son, stem tuber, make crop yield fall sharply, and quality decline is serious.When disease occurs serious, crop is caused to be had no harvest.Cause This, plant pest is of virtually important prevention and treatment meaning in production, chemical control be prevention and treatment plant pest it is the most economical, Quick and effective method.
In actual production, plant disease often occurs simultaneously, such as kind biography, soil-borne disease, foliage disease are usually simultaneously Occur, single use fungicide, not can be well solved the plant disease harm problem of production in practice.By different fungicide Mixture, can remedy such and insufficient, the two has complementary advantages, and can expand prevention spectrum, comprehensive effective disease and pest control harm is real in production Become well received application form in trampling.Fungicide especially with synergy interacts between each active principle, makes Combined effect thus can play a multiplier effect in actual use better than the theoretical summation that effect is used alone, more by User's favor.
Fluorine azoles bacterium acyl azanol (exploitation code name SYN545974；Trade name Adepidyn) it is by Syngenta discovery, exploitation and life The pyrazol acid amide fungicide of production.Fluorine azoles bacterium acyl azanol efficiently, wide spectrum, be suitable for cereal, corn and soybean, rape, vegetables and spy Kind crop etc., prevents and treats many diseases including gray mold, brown spot, leaf spot, sclerotiniose, powdery mildew, head blight etc..Fluorine Azoles bacterium acyl azanol is to leaf spot and powdery mildew activity highest；To the disease for being difficult to prevent and treat, such as botrytis, sclerotinite and stick spore bacterium Disease caused by equal pathogens is efficient；The disease as caused by sickle-like bacteria on cereal, such as head blight are prevented and treated also breakthroughly.And And crop yield and income can be improved in fluorine azoles bacterium acyl azanol.
Fluorine bacterium azoles (triflumizole), tetraconazole (tetraconazole), fluoxastrobin (fluxastrobine), cyprodinil (nyprodinil), Boscalid (boscalid), fludioxonil (fludioxonil), Pyrimethanil (pyrimethanil), procymidone (procymidone) are common in the market, killing with good bactericidal effect Microbial inoculum.
The present invention pass through a large amount of recipe determination experimental studies have found that, when fluorine azoles bacterium acyl azanol and fluorine bacterium azoles, tetraconazole, Fluoxastrobin, cyprodinil, Boscalid, fludioxonil, pyrimethanil, one kind of procymidone or one or more medicament any combination When, show good bactericidal effect.Good synergistic effect is shown, actual effect is substantially better than the theory of exclusive use Preventive effect has very extensive application prospect in production.
Summary of the invention:
Composition of the present invention, including active components A and B, the active components A are fluorine azoles bacterium acyl azanol, institute The active constituent B stated is fluorine bacterium azoles, tetraconazole, fluoxastrobin, cyprodinil, Boscalid, fludioxonil, pyrimethanil, rotten mould One of benefit or more than one combinations.
The present composition component A and B can be higher than rest activity with arbitrary proportion mixture, usually a kind of active component content Ingredient, active components A and B mass ratio are 1:100~100:1, preferably 1:50~50:1.
The composition further includes in addition to the active ingredient (s the auxiliary agent and carrier that can be used in Pesticide formulation.
The composition good can be applied to prevent the plant disease of various crops.
The crop includes but is not limited to conventional crop, especially suitable for prevention and treatment wheat, rice, cereal, corn, big The crops such as beans, rape, vegetables are cooked kind of a disease, such as kind biography, soil-borne disease, foliage disease.
The auxiliary ingredients for pesticide preparations includes auxiliary agent and carrier.
The carrier is one of water, solvent or filler, two kinds or three kinds, the preferred deionized water of water.
The solvent is selected from n,N-Dimethylformamide, cyclohexanone, toluene, dimethylbenzene, dimethyl sulfoxide, methanol, second Alcohol, trimethylcyclohexanone, n-octylpyrrolidone, ethanol amine, triethanolamine, isopropylamine.N-Methyl pyrrolidone, propyl alcohol, One of butanol, ethylene glycol, diethylene glycol, ethylene glycol monomethyl ether, butyl ether, ethanol amine, isopropylamine, ethyl acetate or acetonitrile are more The mixture of kind composition.
The filler is in kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or precipitated calcium carbonate The mixture of one or more compositions.
The auxiliary agent includes at least a kind of surfactant and can be also added antifreeze according to different use occasion and demand The other functions auxiliary agent such as agent, thickener, stabilizer, disintegrating agent, defoaming agent.
The surfactant is selected from one of emulsifier, dispersing agent, wetting agent or bleeding agent or four kinds.Above-mentioned surface Activating agent is the single dose or compound preparation of common nonionic surfactant or anionic surfactant.
Other function auxiliary agent one kind or five in antifreezing agent, thickener, stabilizer, disintegrating agent or defoaming agent Kind.
The emulsifier is selected from agriculture breast 500# (alkyl benzene calcium sulfonate), OP series phosphate (nonylphenol polyoxyethylene ether phosphorus Acid esters), 600# phosphate (phenyl phenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate salt, alkyl biphenyl ether Disulfonic acid magnesium salts, triethanolamine salt, agriculture breast 400# (benzyl dimethyl phenol polyoxyethyl ether), Nongru-700 # (alkylphenol formaldehyde tree Rouge polyoxyethyl ether), peaceful cream 36# (phenethyl phenol formaldehyde resin polyoxyethyl ether), agriculture breast 1600# (phenethyl phenol polyoxyethanyl Polypropylene-base ether), ethylene oxide-propylene oxide block copolymer, OP serial (nonylphenol polyoxyethylene ether), the serial (castor of BY Sesame oil polyoxyethylene ether), agriculture breast 33# (alkylaryl polyoxyethylene poly-oxygen propylene aether), (sorbitol anhydride list is stearic for Span series Acid), one of TWEEN Series (anhydrous sorbitol resin acid ester APEO) or AEO serial (fatty alcohol polyoxyethylene ether) or The mixture of a variety of compositions of person.
The dispersing agent be selected from polycarboxylate, lignosulfonates, APES formaldehyde condensation products sulfat, Alkyl benzene calcium sulfonate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkyl phenol polyoxyethylene ether, aliphatic amine polyoxyethylene ether, fatty acid polyglycol The mixture of one of ethylene oxide ether or glycerol fatty acid ester polyoxyethylene ethe or a variety of compositions.
The wetting agent is selected from lauryl sodium sulfate, neopelex, Nekal BX, wetting and penetrating agent F, soap The mixture of one of angle powder, silkworm excrement or spaonin powder or a variety of compositions.
The bleeding agent is selected from penetrating agent JFC (fatty alcohol polyoxyethylene ether), penetrant t (diisooctyl maleate Sulfonate), the mixture of one of azone or organosilicon or a variety of compositions.
The antifreezing agent is selected from the mixture of one of ethylene glycol, propylene glycol, glycerine or urea or a variety of compositions.
The thickener be selected from one of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or aluminium-magnesium silicate or The mixture of a variety of compositions.
The stabilizer is selected from one kind or more of epoxidized soybean oil, epoxychloropropane, BHT, ethyl acetate, triphenyl phosphate The mixture of kind composition.
The disintegrating agent is selected from bentonite, urea, ammonium sulfate, aluminium chloride, low-substituted hydroxypropyl cellulose, lactose, lemon The mixture of one of acid, succinic acid or sodium bicarbonate or a variety of compositions.
The defoaming agent is selected from silicone oil, silicone compound, C10~C20 saturated fat acids compound or C8~C10 rouge The mixture of one of fat alcohol compound or a variety of compositions.The auxiliary agent and carrier includes but is not limited to described above Auxiliary agent and carrier.
The effective component and auxiliary agent, carrier can be enough by business.
Compared with prior art, the present invention having the following technical effect that
1, mixed composition has good synergistic effect, and application effect is substantially better than single dose theoretical effect summation, that is, has There is super superposition, can preferably control plant disease occurrence injury；
2, there is good complementarity in control of plant disease spectrum between mixed composition active constituent, can expands very well Prevention spectrum, synthesis effectively control various plant disease occurrence injuries；
3, mixed composition has good synergistic effect, can reduce pharmaceutical use, reduces use cost and environment is dirty Dye improves the safety to crop.
Specific embodiment
One, embodiment is prepared
Specific implementation case of the present invention, including but not limited to case study on implementation in detail below, it is all in the application skill It is made equivalent exchange on the basis of art scheme and each falls within protection scope of the present invention.
1, missible oil:
Preparation method: active components A and B are added in carrier in Example formulations ratio, and it is living that surface is added wherein Property agent and other function auxiliary agent, are uniformly mixed in being stirred kettle.Being stirred kettle can be selected enamel reaction Kettle, stainless steel stirred tank etc..
Embodiment 1:30% missible oil (1:2)
Formula: active components A, 10%；Active constituent B, 20%；Emulsifier, 5% and 8%；Solvent, 15%；Solvent, it is remaining Amount.
2, seed treatment suspending agent
Preparation method: active constituent and wetting agent, dispersing agent, antifreezing agent, coloring agent and water are put into stirred tank, sufficiently After stirring, material is pumped into sand mill and is fully ground, after the completion of grinding, is pumped into high-speed shearing machine, thickener is added Afterwards, high speed shear is carried out, seed treatment suspending agent is obtained after the completion of shearing.
Embodiment 2:18% seed treatment suspending agent (1:2)
Formula: active components A 6%, active constituent B are 12%, wetting agent 5%, dispersing agent 6%, and antifreezing agent 5% increases Thick dose 0.2%, coloring agent 0.8%, water complements to 100%.
3, water dispersible granules
Preparation method: in Example formulations ratio, active constituent is added in carrier, and surfactant is added wherein With other function auxiliary agent, mixing adds 10~25% water after air-flow crushing, then makes through kneading, granulation, drying, screening Obtain water dispersible granules product；Or the powder that crushed is sprayed water in boiling granulator, is granulated, is dry, screening later, which is made, to be produced Product.Capital equipment has mixing machine, airslide disintegrating mill, kneader, a Squeezinggranulator, drying chamber or fluidized bed drying or fluidized granulating Machine, screening machine.
Embodiment 3:70% water dispersible granules (2:5)
Formula: active components A 20%, active constituent B are 50%, wetting agent 7%, dispersing agent 5%, and disintegrating agent 5% sticks Agent 0.5% is tied, filler complements to 100%.
4, aqueous emulsion
Preparation method: active constituent and solvent, emulsifier, cosolvent are added together in Example formulations ratio, made molten Solution is at uniform oily phase；By part water, antifreeze, the others insecticides adjuvant such as antimicrobial mixes into uniform water Phase；The oil phase is added to the aqueous phase while being stirred in the reactor at high speed, and slowly plus water is until reach phase inversion point, open cutter into Row high speed shear, and remaining water is added, shearing about half an hour, form the aqueous emulsion of oil-in-water type.
Embodiment 4:12% aqueous emulsion (1:2)
Formula: active components A 4%, active constituent B are 8%, solvent 11%, emulsifier 9%, antifreezing agent 4%, and water is mended Enough to 100%.
5, microemulsion:
Preparation method: in Example formulations ratio, active constituent is completely dissolved with solvent and cosolvent, is then added Other auxiliary agents are uniformly mixed, are eventually adding water, after being sufficiently stirred, can be prepared by microemulsion formulation of the present invention.
Embodiment 5:24% microemulsion (3:1)
Formula: active components A 18%, active constituent B are 6%, solvent 15%, emulsifier 14%, antifreezing agent 5%, water Complement to 100%.
6, wettable powder
Preparation method: in Example formulations ratio, active constituent is added in carrier, and surfactant is added wherein With other function auxiliary agent, mixing remixes obtained wettable powder after air-flow crushing.Capital equipment has mixing machine, air-flow Pulverizer.
Embodiment 6:50% wettable powder (2:3)
Formula: active components A 20%, active constituent B are 30%, and wetting agent 5%, dispersing agent 6%, filler complements to 100%.
7, granule:
Preparation method: active constituent, binder, carrier are weighed in required ratio and is mixed, mixes, adds with carrier after crushing Enter binder stirring granulation, is packed after the assay was approved to get product is arrived.
Embodiment 7:6% granule (1:1)
Formula: active components A 3%, active constituent B are 3%, wetting agent 0.6%, dispersing agent acid 0.9%, colorant 0.2%, filler complements to 100%.
8, suspension seed-coating agent
The preparation method comprises the following steps: by active constituent and wetting agent, dispersing agent, antifreezing agent, warning coloration, film forming agent and water investment stirring In kettle, after being sufficiently stirred, it is pumped into high-speed shearing machine, carries out high speed shear, then pump and be sufficiently sanded into sand mill, be sanded Thickener is added after qualification, stirs evenly up to suspension seed-coating agent.
Embodiment 8:22% suspension seed-coating agent (13:9)
Formula: active components A 13%, active constituent B are 9%, wetting agent 3%, dispersing agent 6%, film forming agent 0.6%, Antifreezing agent 5%, thickener 0.3%, coloring agent 0.8%, water complements to 100%.
Two, active case
Active case 1: 1 test method of bioactivity of fluorine azoles bacterium acyl azanol and fluorine bacterium azoles to cucumber charcoal powdery mildew: reference " the 11st part of NY/T 1156.11-2008 farm-chemical indoor determination test rule fungicide: prevention and treatment cucurbits powdery mildew tests basin Hydroponics " it carries out.
2 test targets: powdery mildew of cucumber (Erysiphe cichoracearum) is for trying pathogen.
3 experimental designs:
Test material prepares: selecting cucumber variety " the close thorn in Shandong " is experimental cultivar, potting culture to 2~4 true leaves, number It is spare.
4 test medicines: fluorine azoles bacterium acyl azanol raw medicine (Adepidyn, Swiss Syngenta Crop Protection Co., Ltd.), fluorine bacterium Azoles (riflumizole, Jiangsu grass biochemistry Co., Ltd, 95%).
5 test procedures:
The preparation of 5.1 spore suspensions:
With the pure water added with a small amount of surfactant, the Fresh spores for taking and covering on Powdery Mildew plant leaf blade are washed, are used Double gauze filtering, it is 1 × 10 that spore concentration, which is made,5The suspension of a spore/ml, it is spare.
5.2 chemicals treatments:
Configured medicament is sprayed on blade to whole wettings, it is spare after medical fluid natural air drying.3 basins of every processing, 4 times It repeats.
5.3 inoculations and culture:
After 72 hours, with spore suspension spray inoculation.Natural air drying after inoculation, then moves to thermostatic chamber, in temperature It is cultivated 7~10 days under conditions of 20~24 DEG C.
5.4 investigation:
When blank control disease leaf rate reaches 80% or more, incidence is managed in classification investigation everywhere, and fork is at least jumped in every processing 30 leaves, grade scale are as follows:
0 grade: disease-free；
1 grade: lesion area accounts for 5% or less full wafer leaf area；
3 grades: lesion area accounts for the 5%~15% of full wafer leaf area；
5 grades: lesion area accounts for the 15%~25% of full wafer leaf area；
7 grades: lesion area accounts for the 25%~50% of full wafer leaf area；
9 grades: lesion area accounts for the 50%~75% of full wafer leaf area；
11 grades: lesion area accounts for 75% or more of full wafer leaf area；
6 data statistic analysis
6.1 calculation methods:
According to survey data, the disease index and control efficiency of each processing are calculated.
6.1.1 disease index:
It is calculated by calculation formula, as a result retains after decimal point 2.
In formula:
X-disease index；
Ni-sick the numbers of sheets at different levels；
I-is with respect to value of series；
N-investigation total number of sheets；
11-highest values of series.
6.1.2 control efficiency:
It is calculated by formula, calculated result retains after decimal point 2.
In formula:
P-control efficiency, unit are percentage (%)；
CK-blank control disease index；
PT-chemicals treatment disease index.
6.2 statistical analysis:
The EC50 value, related coefficient, virulence regression equation and corresponding value of every kind of medicament are calculated with DPS software.
The synergistic effect of pharmacy mix, i.e. CTC are evaluated according to the co-toxicity coefficient method (CTC) of (1960) Sun&Johnson ≤ 80 be antagonism, and 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect.
Survey toxicity index calculation formula:
Mixtures theoretical toxicity index calculation formula
TTI=TTIA×XA+TTIB×XB
In formula:
TTI: mixtures theoretical toxicity index
TTIA: the survey toxicity index of mixture Chinese medicine A
XA: the percentage composition of mixture Chinese medicine A
TTIB: the survey toxicity index of mixture Chinese medicine B
XB: the percentage composition of mixture Chinese medicine B
Mixture co-toxicity coefficient calculation formula
6 conclusion (of pressure testing)s:
Fluorine azoles bacterium acyl azanol, fluorine bacterium azoles and the two different ratio mixture are to cucumber powdery mildew bacteria joint toxicity measuring, respectively The EC50 value of medicament, virulence regression equation, related coefficient, co-toxicity coefficient (CTC) are shown in Table 1.
1 fluorine azoles bacterium acyl azanol of table and fluorine bacterium azoles mixture are to cucumber powdery mildew bacteria co-toxicity interpretation of result
Show according to test data and (be shown in Table 1) as it can be seen that fluorine azoles bacterium acyl azanol and fluorine bacterium azoles prevent and treat room for powdery mildew of cucumber Interior co-toxicity coefficient is synergy and summation action.
Active case 2: fluorine azoles bacterium acyl azanol and Boscalid test the toxicity of grape grey mould
1 test basis: NY/T1156.9-2008 " the 9th part of farm-chemical indoor determination test rule fungicide: inhibits Ash arrhizus bacteria pilot blade method "
2 for examination target and condition of culture
Grape grey mould (Botrytis cinerea) acquires fresh disease from Jimo City of Qingdao City shop Yi Feng greenhouse controlling garden Fringe, isolation and purification culture, the condition of culture of pathogen are 25 ± 5 DEG C of temperature, relative humidity 65 ± 5%.
3 experimental designs
The processing of 3.1 reagents
3.1.1 test medicine
95% fluorine azoles bacterium acyl azanol raw medicine, 98% Boscalid raw medicine,
3.1.2 medicament mother liquor preparation method
Fluorine azoles bacterium acyl hydroxylamine solution: weighing 95% fluorine azoles bacterium acyl azanol raw medicine, is first configured to 1000mg/L mother with DMF dissolution Liquid, be then added and be diluted to 10 containing 0.1% Tween 80 aqueous solution, 20,40,80,160mg/L concentration it is spare；
Boscalid solution: weighing 98% Boscalid raw medicine, is first configured to 1000mg/L mother liquor with DMF dissolution, then Be added and be diluted to 20 containing 0.1% Tween 80 aqueous solution, 40,80,160,320mg/L concentration it is spare；
Contrast solution: the aqueous solution containing same amount of solvent and emulsifier contained in maximum concentration.
3.2 test process
3.2.1 dosage is arranged
Fluorine azoles bacterium acyl azanol is mixed with Boscalid to set 5 groups of proportions altogether, on the basis of preliminary experiment, each single dose and Each proportion sets the processing of 5 concentration respectively, using maximum concentration handle contained in solvent and the amount of emulsifier as blank control, Specific concentration processing is as follows:
(10 times of liquid that the concentration is required concentration)
Fluorine azoles bacterium acyl azanol 10,20,40,80,160mg/L
Boscalid 20,40,80,160,320mg/L
Fluorine azoles bacterium acyl azanol: Boscalid (4:1) 10,20,40,80,160mg/L
Fluorine azoles bacterium acyl azanol: Boscalid (2:1) 10,20,40,80,160mg/L
Fluorine azoles bacterium acyl azanol: Boscalid (1:1) 10,20,40,80,160mg/L
Fluorine azoles bacterium acyl azanol: Boscalid (1:2) 10,20,40,80,160mg/L
Fluorine azoles bacterium acyl azanol: Boscalid (1:4) 10,20,40,80,160mg/L
The amount of solvent and emulsifier contained in the processing of blank control maximum concentration
3.2.2 test repeats
Each concentration processing is repeated 4 times, each 3 ware of repetition.
4 test methods
Referring to biometric standard method NY/T1156.9-2008, using pastille culture medium method: taking each single dose and mixture series dense The medical fluid 4ml of degree is added in the PDA culture medium for the 36mL for being cooled to 45 DEG C, and the pastille culture medium of final concentration required for being made is flat Plate.Then 6mm diameter mycelia block is produced from the Botrytis cinerea colony edge of culture 7 days, moves to each serial pastille culture medium On, mycelia is face-down, 4 repetitions of every processing.It is disposed, is placed in 28 DEG C of constant temperature biochemical cultivation case and cultivates, to clear water pair According to culture dish in mycelia grow to the 2/3 of culture dish when, measure colony diameter, calculate growth inhibition ratio.
5 data survey and statistical analysises
5.1 investigation method
Test process measures colony diameter after 4 days, calculate growth inhibition ratio (%).
D=D1-D2
In formula: D indicates that bacterium colony increases diameter；D1Indicate colony diameter；D2Indicate bacteria cake diameter.
I (%)=[(D0-Dt)/D0]*100
In formula: I indicates mycelial growth inhibition rate；D0Indicate that blank control bacterium colony increases diameter；DtIndicate chemicals treatment bacterium colony Increase diameter.
5.2 synegism evaluation methods
Referring to biometric standard method NY/1156.9-2008, according to the co-toxicity coefficient method (CTC) of (1960) Sun&Johnson Evaluate the synergistic effect of pharmacy mix, i.e., CTC≤80 are antagonism, 80 < CTC < 120 are summation action, CTC >=120 For synergistic effect.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)*100
Percentage composition+medicament B of A medicament in mixtures theoretical toxicity index (TTI)=medicament A toxicity index * mixture The percentage composition of B medicament in toxicity index * mixture
Co-toxicity coefficient (CTC)=(mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI)) * 100
5.3 data statistic analysis
All test datas are all made of SAS6.12 statistical software and are analyzed.Mycelia growth suppression is calculated according to test data Rate (%) processed, finds out virulence regression equation, related coefficient (r) and EC50。
6 interpretations of result and discussion
6.1 evaluating drug effect
Fluorine azoles bacterium acyl azanol combines the virulence to Botrytis cinerea with the mixture of Boscalid and the two different proportion Measurement result is shown in Table 1.Seen from table 1, fluorine azoles bacterium acyl azanol and Boscalid are with 5 mixtures of 4:1,2:1,1:1,1:2,1:4 Combination shows as synergistic effect, EC to Botrytis cinerea50Value is respectively 1.9971,1.6541,1.7826,1.9605 And 2.2905mg/L, co-toxicity coefficient are respectively 236.06,299.54,296.86,289.64 and 263.30, wherein with 2:1 ratio Mixture is most obvious to Botrytis cinerea synergistic effect.
2 fluorine azoles bacterium acyl azanol of table is with Boscalid mixture to the toxicity test result of Botrytis cinerea
According to experiments have shown that (being shown in Table 2) indoor biometrics the result shows that, fluorine azoles bacterium acyl azanol and Boscalid with 4:1,2:1, 1:1,1:2 and 1:4 ratio carry out mixture, the results showed that and each ratio mixture all shows synergistic effect to Botrytis cinerea, wherein It is best with the synergistic effect of 2:1.
Active case 3: fluorine azoles bacterium acyl azanol and tetraconazole test apple marssonina leaf spot toxicity
1 for examination target and condition of culture
Marssonina coronaria (Marssonina coronaria) is saved and is mentioned by Qingdao Agricultural University's plant pathology room For the condition of culture for target after examination target and test is 25 ± 5 DEG C of temperature, relative humidity 65 ± 5%.
2 experimental designs
2.1 reagent
2.1.1 test medicine
97.0% fluorine azoles bacterium acyl azanol raw medicine；95.0% tetraconazole raw medicine
2.1.2 medicament mother liquor preparation method
Fluorine azoles bacterium acyl azanol mother liquor: 0.1031g fluorine azoles bacterium acyl azanol raw medicine is weighed, 2.00gN, N- dimethyl methyl are dissolved in Amide is added the aqueous solution containing 0.1%Tween80 and supplies 100ml, stirs evenly, be configured to 1000mg/L mother liquor.
Tetraconazole mother liquor: weighing 0.1053g tetraconazole raw medicine, is dissolved in 2.00gN, and N- dimethylformamide adds Enter the aqueous solution containing 0.1%Tween80 and supply 100ml, stir evenly, is configured to 1000mg/L mother liquor.
Blank control mother liquor: weighing n,N-Dimethylformamide 2.00g, and the aqueous solution containing 0.1%Tween80 is added and mends Sufficient 100mL.
2.2 test process
2.2.1 dosage is arranged
Fluorine azoles bacterium acyl azanol totally 5 groups of proportions mixed with tetraconazole, on the basis of preliminary experiment, each single dose and each Proportion sets 5 concentration processing respectively, using the system without medical fluid as blank control (maximum concentration referring to chemicals treatment dilutes). Specific concentration processing is as follows:
Fluorine azoles bacterium acyl azanol 0.05,0.1,0.2,0.4,0.8mg/L
Tetraconazole 0.05,0.1,0.2,0.4,0.8mg/L
Fluorine azoles bacterium acyl azanol: tetraconazole (4:1) 0.05,0.1,0.2,0.4,0.8mg/
L fluorine azoles bacterium acyl azanol: tetraconazole (2:1) 0.05,0.1,0.2,0.4,0.8mg/
L fluorine azoles bacterium acyl azanol: tetraconazole (1:1) 0.05,0.1,0.2,0.4,0.8mg/
L fluorine azoles bacterium acyl azanol: tetraconazole (1:2) 0.05,0.1,0.2,0.4,0.8mg/
L fluorine azoles bacterium acyl azanol: tetraconazole (1:4) 0.05,0.1,0.2,0.4,0.8mg/
L using maximum concentration handle contained in solvent and 0.1%Tween80 aqueous solution as blank control.
2.2.2 test repeats
Each concentration processing is repeated 4 times, 3 wares of every repetition.
2.3 processing times and number:
Medication is primary.
3 test methods
Referring to biometric standard method NY/T1156.2-2008, using mycelial growth rate method: by above two single dose and mixing Agent is configured to above-mentioned required 10 times of concentration respectively, takes the medical fluid 3mL of each single dose and mixture series of concentrations, and addition is cooled to 45 DEG C 27mL PDA culture medium in, be made required for final concentration pastille culture medium plate.Then from cultured apple tree foxiness Germ colony edge produces 6mm mycelia block, moves in each serial pastille culture medium, mycelia is face-down, 4 repetitions of every processing.Place Reason finishes, and is placed in 28 DEG C of constant temperature biochemical cultivation case and cultivates, and colony diameter is measured after 4 days, calculates growth inhibition ratio.
4 data survey and statistical analysises
4.1 investigation method
Test process measures colony diameter after 4 days, calculate growth inhibition ratio (%).
D=D1-D2
In formula: D indicates that bacterium colony increases diameter；D1 indicates colony diameter；D2 indicates bacteria cake diameter.
I (%)=((D0-Dt)/D0) * 100
In formula: I indicates mycelial growth inhibition rate；D0 indicates that blank control bacterium colony increases diameter；
Dt indicates that chemicals treatment bacterium colony increases diameter.
4.2 synegism evaluation methods
Referring to biometric standard method NY/1156.6-2006, according to the co-toxicity coefficient method (CTC) of (1960) Sun&Johnson Evaluate the synergistic effect of pharmacy mix, i.e., CTC≤80 are antagonism, 80 < CTC < 120 are summation action, CTC >=120 For synergistic effect.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100 mixtures theoretical toxicity indexes (TTI) B in percentage composition+medicament B toxicity index * 100* mixture of A medicament in=medicament A toxicity index * 100* mixture The percentage composition co-toxicity coefficient (CTC) of medicament=(mixture surveys toxicity index (ATI)/mixture theory virulence refers to
Number (TTI)) * 100
4.3 data statistic analysis
All test datas are all made of SAS6.12 statistical software and are analyzed.Mycelia growth suppression is calculated according to test data Rate (%) processed, finds out virulence regression equation, related coefficient (r) and EC50.
5 test results and analysis
3 fluorine azoles bacterium acyl azanol of table is with tetraconazole mixture to the toxicity test result fluorine azoles bacterium acyl hydroxyl of apple tree brown patch germ Amine, tetraconazole and the two different ratio mixture are to apple tree brown patch germ indoor toxicity test, the EC of each medicament50It is worth and corresponding B value and virulence regression equation be shown in Table 3.As can be seen from Table 2, fluorine azoles bacterium acyl azanol is low to the virulence of apple tree brown patch germ In tetraconazole, their EC50Value is respectively 0.1901mg/L and 0.1751mg/L.Fluorine azoles bacterium acyl azanol and tetraconazole are mixed Match, corresponding co-toxicity coefficient when matching as 4:1,2:1,1:1,1:2,1:4 is respectively 114.31,130.63,138.04, 120.79,110.65, show that 2:1,1:1,1:2 proportion mixture show a degree of synergy to apple tree brown patch germ and makees With 4:1,1:4 proportion mixture show as summation action to apple tree brown patch germ.
Active case 4: fluorine azoles bacterium acyl azanol and pyrimethanil are to gray mold of cucumber toxicity case
1 for trying target
Botrytis cinerea pers (Botrytis cinerea), diseased plant pick up from Jimo City base Yi Feng cucumber disease melon, train in PDA It supports and carries out tissue separation and pathogen purifying on base according to a conventional method, obtain pure culture bacterial strain.
2 condition of culture
Bacterial strain in PDA culture medium preculture 4 days it is spare, 25 ± 5 DEG C of temperature, relative humidity 65 ± 5%.
3 experimental designs
3.1 reagent
3.1.1 test medicine
95.0% raw medicine of fluorine azoles bacterium acyl azanol；95.0% raw medicine of pyrimethanil.
3.1.2 other reagents and medicament mother liquor method
Fluorine azoles bacterium acyl azanol: 0.1053g raw medicine is weighed, 2.00gN- dimethylformamide is dissolved in, uses 0.1%Tween80 Aqueous solution dilutes and is settled to 100mL, stirs evenly, and is configured to 1000mg/L mother liquor.
Pyrimethanil: weighing 0.1053g raw medicine, is dissolved in 2.00gN- dimethylformamide, water-soluble with 0.1%Tween80 Liquid dilutes and is settled to 100mL, stirs evenly, and is configured to 1000mg/L mother liquor.
Blank control mother liquor: weighing dinethylformamide 2.00g, is diluted and is settled to 0.1%Tween80 aqueous solution 100mL。
3.2 test process
3.2.1 dosage is arranged
Fluorine azoles bacterium acyl azanol and pyrimethanil mixture set 5 groups of mixture combinations altogether, separately set fluorine azoles bacterium acyl azanol and pyrimethanil two A single dose control and a blank control, each single dose and each mixture combination set 5 concentration processing respectively, at maximum concentration The solution of contained solvent and 0.1%Tween80 aqueous solution does blank control in reason.Specific concentration processing is as follows:
Fluorine azoles bacterium acyl azanol 1,2,4,8,16mg/L
Pyrimethanil 1,2,4,8,16mg/L
Fluorine azoles bacterium acyl azanol: pyrimethanil (4:1) 1,2,4,8,16mg/L
Fluorine azoles bacterium acyl azanol: pyrimethanil (2:1) 1,2,4,8,16mg/L
Fluorine azoles bacterium acyl azanol: pyrimethanil (1:1) 1,2,4,8,16mg/L
Fluorine azoles bacterium acyl azanol: pyrimethanil (1:2) 1,2,4,8,16mg/L
Fluorine azoles bacterium acyl azanol: pyrimethanil (1:4) 1,2,4,8,16mg/L
The solution of contained solvent and 0.1%Tween80 aqueous solution in the processing of blank control maximum concentration
3.2.2 test repeats
Each concentration processing is repeated 4 times.
3.3 processing times and number
Medication is primary.
4 test methods
The test basis People's Republic of China (PRC) agricultural industry criteria (fungicide) NY/T 1156.3-2006, NY/T 1156.6-2006 is carried out using mycelial growth rate method.
4.1 chemicals treatment
Above two single dose and 5 kinds of mixed combination medicaments are configured to above-mentioned required 10 times of concentration respectively, take 1mL medicament Addition 9mL, which is cooled in about 50 DEG C of PDA culture medium, to be mixed, and pours into and drug containing tablet is made in culture dish.
4.2 inoculations and culture
The germ of preculture in PDA culture medium is broken into bacteria cake with punch (diameter=6mm), is inoculated in drug containing tablet Centre, 4 repetitions are placed in culture 3 days in 25 DEG C of constant incubators.
5 data survey and statistical analysises
5.1 investigation method
Colony diameter, and control are handled using crossing method measurement different agents and various concentration after test process 3 days Compare and calculates growth inhibition ratio with following equation.
5.2 calculation method
D=D1-D2
In formula: D indicates that bacterium colony increases diameter；D1 indicates colony diameter；D2 indicates bacteria cake diameter.
I (%)=[(D0-Dt)/D0] * 100
In formula: I indicates mycelial growth inhibition rate；D0 indicates that blank control bacterium colony increases diameter；Dt indicates chemicals treatment bacterium Fall growth diameter.
The EC50 value, related coefficient, virulence regression equation and corresponding value of every kind of medicament are calculated with DPS software.
Referring to biometric standard method NY/1156.6-2006, according to the co-toxicity coefficient method (CTC) of (1960) Sun&Johnson Evaluate the synergistic effect of pharmacy mix, i.e., CTC≤80 are antagonism, 80 < CTC < 120 are summation action, CTC >=120 For synergistic effect.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
Percentage composition+medicament of A medicament in mixtures theoretical toxicity index (TTI)=medicament A survey toxicity index * mixture The percentage composition co-toxicity coefficient (CTC) of B medicament=[mixture surveys toxicity index (ATI)/mixed in the survey toxicity index * mixture of B Agent theoretical toxicity index (TTI)] * 100
6 test results and analysis
Fluorine azoles bacterium acyl azanol, pyrimethanil and the two different ratio mixture are to botrytis cinerea pers joint toxicity measuring, each medicine The EC50 value of agent, virulence regression equation, related coefficient, co-toxicity coefficient (CTC) are shown in Table 4.
4 fluorine azoles bacterium acyl azanol of table and pyrimethanil mixture are to botrytis cinerea pers co-toxicity interpretation of result
Reagent agent Virulence regression equation Related coefficient EC50(mg/L) Co-toxicity coefficient
Fluorine azoles bacterium acyl azanol Y=1.2812x+4.0312 0.9958 5.7036 —
Pyrimethanil Y=1.1310x+4.3229 0.9928 3.9692 —
Fluorine: phonetic (4:1) Y=1.1240x+4.2630 0.9959 4.5256 115.90
Fluorine: phonetic (2:1) Y=1.1297x+4.3227 0.9967 3.9769 125.18
Fluorine: phonetic (1:1) Y=1.1055x+4.3875 0.9951 3.5815 130.70
Fluorine: phonetic (1:2) Y=1.0619x+4.4617 0.9924 3.2131 137.47
Fluorine: phonetic (1:4) Y=1.0739x+4.4600 0.9918 3.1829 132.78
Table 4 is as can be seen that pyrimethanil is higher than fluorine azoles bacterium acyl azanol, their EC to the virulence of botrytis cinerea pers50Value point It Wei not 3.9692mg/L and 5.7036mg/L.Fluorine azoles bacterium acyl azanol and pyrimethanil mixture, match as 4:1,2:1,1:1,1:2,1:4 When corresponding co-toxicity coefficient be respectively 115.90,125.18,130.70,137.47,132.78, show each proportion mixture to cucumber Ash arrhizus bacteria shows better effect.Wherein match fluorine azoles bacterium acyl azanol: the synergistic effect of pyrimethanil (1:2) is larger.
Active case 5: fluorine azoles bacterium acyl azanol is with procymidone mixture to the field control effectiveness test of sclerotinia sclerotiorum
1 test method: according to GB/T17980.35-200 " pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating oil Dish sclerotiniose " it carries out.
2 subjects: miscellaneous No. four of rape variety-China.
3 experimental designs and arrangement:
3.1 medicament
3.1.1 test medicine: fluorine azoles bacterium acyl azanol and procymidone different ratio, the total setting processing 1 of different dosages, place Reason 2, processing 3 ... processing 7 amount to 7 processing.
3.1.2 comparison medicament: fluorine azoles bacterium acyl azanol, procymidone single dose comparison medicament, blank control.
3.2 cell arrangements
3.2.1 cell arranges
Test medicine, comparison medicament and the processing of the cell of blank control are arranged using random district's groups.
3.2.2 plot area and repetition
Plot area: 20 square metres/are repeated 4 times.
3.3 insecticide-applying way: spraying.
3.4 application periods and number
Rape full blossom initial stage, rape flowering are respectively administered 1 time.
4 investigation, record and measurement method
4.1 investigation methods, time and number
4.1.1 investigation method
10 points of every cell checkerboard type investigation, 5 plants (50 plants/cell) of every investigation.
0 grade: disease-free；
1 grade: slight morbidity, onset area account for 5% or less stem surface area；
3 grades: slight morbidity, onset area account for the 6%~15% of stem surface area；
5 grades: medium morbidity, onset area account for the 16%~30% of stem surface area；
7 grades: height is fallen ill, and onset area accounts for the 31%~50% of stem surface area；
9 grades: serious morbidity, onset area account for 50% or more of stem surface area；
4.1.2 survey plan and number
Rape florescence at end, silique phase investigate stem disease strain rate, disease index respectively.
4.1.3 drug effect calculation method
Drug effect calculation formula
In formula:
Ck: disease index after blank district application
PT: chemicals treatment district is will rear disease index
5 results and analysis
5 fluorine azoles bacterium acyl azanol of table and procymidone are to sclerotinia sclerotiorum test result
(be shown in Table 5) as the result is shown according to field efficacy: each chemicals treatment is to the preventive effect of sclerotinia sclerotiorum at whole florescence and angle Fruiting period is above single dose processing and control treatment.
Active case 6: fluorine azoles bacterium acyl azanol is with fluoxastrobin mixture to the field efficacy of rice blast
1 test method: according to GB/T17980.19-2000 " pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating water Rice leaf diseases " criterion development test.
2 experimental conditions
The selection of 2.1 subjects, crop and kind
Subjects: rice blast (Pyricularia oryzae)
For studying object: rice, kind are two excellent 287
2.2 arable farmings and environmental condition
Experimental field soil is clay, and middle fertility, field management level uniformity, field humidity is moister, surrounding soil Ground long-term cropping is rice.
3 experimental designs and arrangement
3.1 medicament
3.1.1 test medicine
Fluorine azoles bacterium acyl azanol and fluoxastrobin different disposal amount to tetra- processing of ABCD.
3.1.2 comparison medicament
Fluorine azoles bacterium acyl azanol (E), fluoxastrobin single dose (F), blank control (CK)
3.2 plot area and repetition
Plot area: every plot area is 15m2。
Number of repetition: this test sets 7 processing, and 4 repetitions set 28 cells altogether, set 2 row isolation strips between processing district's groups.
3.3 application method
3.3.1 application method
Carry out field spray is watered in rice blast early stage.
3.3.2 spraying time and number
Application three times, is administered interval 7 days altogether.
4 investigation, record and measurement method
4.1 investigation methods, time and number
4.1.1 control time and number
7 days progress efficacy surveys after being administered with last time for 7 days after first time is administered.
4.1.2 investigation method
According to pesticide field efficacy medicine test criterion, field investigation uses diagonal line five point sampling, and every takes 50 fringes, record Sick spike number and sick series.
Rice Panicle neck disease (as unit of fringe) severity Scaling standard:
0 grade: disease-free；
1 grade: every 5% or less fringe loss (individual branch stalk morbidities)；
3 grades: every fringe loss 6~20% (morbidity of 1/3 or so branch stalk)；
5 grades: every fringe loss 21~50% (fringe neck or main shaft morbidity, grain half are flat)；
7 grades: every fringe loss 51~70% (fringe neck morbidity, big portion's shrivelled kernel)；
9 grades: every fringe loss 71~100% (fringe neck morbidity, cause dead ears).
4.1.3 drug effect calculation method
According to pesticide field efficacy medicine test criterion calculation formula, disease index and control efficiency are calculated.
Control efficiency calculation formula is as follows:
5 results and analysis
6 30% fluorine azoles bacterium acyl azanol fluoxastrobin suspending agent of table prevents and treats rice blast test result
As can be seen from Table 6, the reagent agent fluorine azoles bacterium acyl azanol proportion different from fluoxastrobin mixture is harnessed the river for anti- Rice rice blast all has good preventive effect, and preventive effect is 85% or more.It is substantially better than prevention and treatment of the single dose for rice blast.

Claims (6)

1. a kind of composition, which is characterized in that including active components A and B.
2. composition as described in claim 1, which is characterized in that the active components A is fluorine azoles bacterium acyl azanol.
3. composition as described in claim 1, which is characterized in that the active constituent B is fluorine bacterium azoles, tetraconazole, fluorine One of Fluoxastrobin, cyprodinil, Boscalid, fludioxonil, pyrimethanil, procymidone or more than one combinations.
4. composition as described in claim 1, which is characterized in that composition A and the B mass ratio is 1:100~100: 1, preferably 1:50~50:1.
5. composition as claimed in claim 1,2 or 3, which is characterized in that further include pesticide system other than active components A and B The auxiliary agent and carrier that can be used in agent processing.
6. the composition as described in claim 1,2,3 or 4, which is characterized in that be applied to controlling plant diseases.
CN201810926193.1A 2018-08-15 2018-08-15 A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol Pending CN109042676A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109169677A (en) * 2018-10-09 2019-01-11 东莞市瑞德丰生物科技有限公司 A kind of composition and its application of fluorine-containing azoles bacterium acyl azanol and fludioxonil
CN112616844A (en) * 2020-11-27 2021-04-09 浙江威尔达化工有限公司 Bactericide
CN114027325A (en) * 2021-10-14 2022-02-11 广东真格生物科技有限公司 Sterilization composition containing fluconazole hydroxylamine, preparation and application thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109169677A (en) * 2018-10-09 2019-01-11 东莞市瑞德丰生物科技有限公司 A kind of composition and its application of fluorine-containing azoles bacterium acyl azanol and fludioxonil
CN112616844A (en) * 2020-11-27 2021-04-09 浙江威尔达化工有限公司 Bactericide
CN114027325A (en) * 2021-10-14 2022-02-11 广东真格生物科技有限公司 Sterilization composition containing fluconazole hydroxylamine, preparation and application thereof

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