CN109042413A - A method of it reducing receptor shellfish trnasplantion immunity and repels - Google Patents
A method of it reducing receptor shellfish trnasplantion immunity and repels Download PDFInfo
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- CN109042413A CN109042413A CN201811164623.7A CN201811164623A CN109042413A CN 109042413 A CN109042413 A CN 109042413A CN 201811164623 A CN201811164623 A CN 201811164623A CN 109042413 A CN109042413 A CN 109042413A
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- 235000015170 shellfish Nutrition 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 40
- 230000036039 immunity Effects 0.000 title claims abstract description 23
- 239000011049 pearl Substances 0.000 claims abstract description 66
- PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical compound CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 claims abstract description 62
- 229930105110 Cyclosporin A Natural products 0.000 claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 238000007796 conventional method Methods 0.000 claims abstract description 20
- 239000010984 cultured pearl Substances 0.000 claims abstract description 19
- 239000013535 sea water Substances 0.000 claims abstract description 15
- 230000009278 visceral effect Effects 0.000 claims abstract description 6
- 241000879903 Pteria Species 0.000 claims description 18
- PZJWYUDBXNNVLZ-UHFFFAOYSA-N 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxoquinoline-3-carboxylic acid;hydrochloride Chemical compound Cl.C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 PZJWYUDBXNNVLZ-UHFFFAOYSA-N 0.000 claims description 9
- -1 compound amino acid Chemical class 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 6
- 230000008629 immune suppression Effects 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 230000003115 biocidal effect Effects 0.000 claims description 4
- AYIRNRDRBQJXIF-NXEZZACHSA-N (-)-Florfenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CF)NC(=O)C(Cl)Cl)C=C1 AYIRNRDRBQJXIF-NXEZZACHSA-N 0.000 claims description 2
- 229930195503 Fortimicin Natural products 0.000 claims description 2
- 229930182555 Penicillin Natural products 0.000 claims description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 2
- 241000183300 Pteria penguin Species 0.000 claims description 2
- BIDUPMYXGFNAEJ-APGVDKLISA-N astromicin Chemical compound O[C@@H]1[C@H](N(C)C(=O)CN)[C@@H](OC)[C@@H](O)[C@H](N)[C@H]1O[C@@H]1[C@H](N)CC[C@@H]([C@H](C)N)O1 BIDUPMYXGFNAEJ-APGVDKLISA-N 0.000 claims description 2
- 229960003760 florfenicol Drugs 0.000 claims description 2
- 229940049954 penicillin Drugs 0.000 claims description 2
- 230000001846 repelling effect Effects 0.000 claims 6
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 239000003018 immunosuppressive agent Substances 0.000 abstract description 8
- 230000001900 immune effect Effects 0.000 abstract description 5
- 230000006058 immune tolerance Effects 0.000 abstract description 5
- 230000009467 reduction Effects 0.000 abstract description 3
- 230000002980 postoperative effect Effects 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 238000013401 experimental design Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- 206010052779 Transplant rejections Diseases 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 238000005025 nuclear technology Methods 0.000 description 3
- 238000011056 performance test Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 210000001835 viscera Anatomy 0.000 description 3
- 102000004631 Calcineurin Human genes 0.000 description 2
- 108010042955 Calcineurin Proteins 0.000 description 2
- 108010036949 Cyclosporine Proteins 0.000 description 2
- 102000003945 NF-kappa B Human genes 0.000 description 2
- 108010057466 NF-kappa B Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229960001265 ciclosporin Drugs 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 235000013411 Alpinia speciosa Nutrition 0.000 description 1
- 244000060696 Alpinia speciosa Species 0.000 description 1
- 229940122739 Calcineurin inhibitor Drugs 0.000 description 1
- 102100024123 Calcineurin-binding protein cabin-1 Human genes 0.000 description 1
- 101710192106 Calcineurin-binding protein cabin-1 Proteins 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 108010077850 Nuclear Localization Signals Proteins 0.000 description 1
- 241000490567 Pinctada Species 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 229930182912 cyclosporin Natural products 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000030609 dephosphorylation Effects 0.000 description 1
- 238000006209 dephosphorylation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
- A01K61/56—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of methods that reduction receptor shellfish trnasplantion immunity is repelled, belong to seawater pearl culture technical field, immunosuppressor Ciclosporin A is brought into receptor shellfish body by following steps: (1) Ciclosporin A being dissolved in basal liquid and is configured to Ciclosporin A solution；(2) seawater pearl shell mantle piece is made, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated on Ciclosporin A solution；(4) according to a conventional method by mantle piece and pearl renucleation receptor shellfish visceral mass；(5) core position is planted to receptor shellfish using sprayer to be sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls；Immunosuppressor Ciclosporin A is applied to production of growing cultured pearls by the present invention, appropriate to reduce receptor shellfish in the plant postoperative trnasplantion immunity rejection of core, is accelerated receptor shellfish and is converted from immunological rejection to immune tolerance, so that pearl sac be promoted to be formed, improves pearl production.
Description
Technical field
The present invention relates to it is a kind of reduction receptor shellfish trnasplantion immunity repel method more particularly to it is a kind of utilize Ciclosporin A
The method that receptor shellfish trnasplantion immunity is repelled is reduced, seawater pearl culture technical field is belonged to.
Background technique
Artificial culture sea water pearls are the forming process for imitating natural peral, by performing the operation the mantle piece of donor shellfish
Tissue and a circular pearl core are transplanted to together in the visceral mass connective tissue of receptor shellfish.Then, mantle piece tissue with by
Body shellfish tissue interaction, epithelial cell are gradually proliferated, break up, and form the pearl sac for surrounding pearl core.Pearl sac constantly secretes treasure
Pearl matter is deposited on pearl core surface and forms pearl.In production, benefit of growing cultured pearls usually is improved using mantle piece Precerving liquid, it is former
Reason mainly maintains small pieces activity, promotes cell Proliferation and avoids infection.
The implantation process of mantle piece and pearl core is similar to the organ transplant of higher organism, after the transfer initial stage, implantation
Allosome mantle tissue and pearl core can cause the graft-rejection of receptor shellfish, and about 10-15 days after the transfer, a part of receptor shellfish
The small pieces tissue and pearl core for gradually receiving implantation establish immune tolerance, form the pearl sac for surrounding pearl core.In this process, mistake
Strong rejection will lead to the core of spitting of pearl sac dysplasia and receptor shellfish, and even receptor shellfish is dead, to reduce treasure
The quality and yield of pearl.
Therefore, Effective Regulation pearl shell plants the graft rejection after core, facilitates the immune tolerance of pearl shell, is that pearl sector is held
Problem in the urgent need to address in supervention exhibition, and the not yet targeted scheme of existing production technology.
Immunosuppressor is to reduce graft rejection most effectual way, can significantly improve transplant organ survival rate.Ciclosporin A,
Also known as cyclosporin A (cyclosporine, CsA) is a kind of by 11 amino acids formed ring molecules, belongs to calcineurin
Inhibitor family has strong immunosuppressive action, has been widely used in clinical filed of organ transplantation at present, has greatly improved shifting
Plant the survival rate of organ.CsA is by the way that it is compound to form CyP/CsA with rich in combining in intracytoplasmic Ciclosporin A receptor (CyP)
Object, then CyP/CsA/CN ternary complex is formed with 1: 1: 1 ratio with calcineurin (calcineurin, CN), to make
CN dephosphorylation is blocked, Ca2+Interior stream is obstructed, the nuclear localization signal of Nuclear factor kappa B (nuclear factor κ B, NF- κ B)
It cannot expose, therefore close and Signal Transduction Pathways are activated by the nucleus of CN activation, inhibit the activation and proliferation of immunocyte, finally
It is suppressed immunity of organism repulsive interaction.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of methods that reduction receptor shellfish trnasplantion immunity is repelled, and pass through
Mantle piece and pearl core bring Ciclosporin A solution in receptor shellfish body into, the suppression being immunoreacted using Ciclosporin A to receptor shellfish
Production is used, and is accelerated receptor shellfish and is converted from immunological rejection to immune tolerance, promotes mantle piece survival and hyperplasia, to promote
Pearl sac is formed, and core-remaining rate and bead yield rate is improved, to improve pearl production.
Above-mentioned purpose of the invention is achieved by the following technical programs:
A method of it reducing receptor shellfish trnasplantion immunity and repels, this method is mould by immunosuppressor ring spore by following steps
Plain A is brought into receptor shellfish body: (1) Ciclosporin A being dissolved in basal liquid and be configured to Ciclosporin A solution；(2) production seawater is precious
Ciclosporin A solution is added dropwise and infiltrates on mantle piece by pearl shellfish mantle piece；(3) pearl core is infiltrated on Ciclosporin A
Solution；(4) according to a conventional method by mantle piece and pearl renucleation receptor shellfish visceral mass；(5) receptor shellfish is planted using sprayer
Core position is sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls；
Above-mentioned Ciclosporin A solution contains the Ciclosporin A of 0.40%-0.55%；Above-mentioned basal liquid is with every liter of sterilizing sea
Water dissolves 3-5 grams of glucose, and 1-3 grams of compound amino acid, 0.1-0.2 grams of antibiotic configures；Above-mentioned spraying solution is base
Plinth liquid；Above-mentioned receptor shellfish is pteria martensii, large nacre, nacre or Pteria penguin；Above-mentioned antibiotic is penicillin+chain
Mycin, Florfenicol+fortimicin or Enrofloxacin HCL.
The invention has the advantages that immunosuppressor Ciclosporin A is applied to production of growing cultured pearls by this method, it is appropriate to reduce
Receptor shellfish is planting the postoperative trnasplantion immunity rejection of core, accelerates receptor shellfish and converts from immunological rejection to immune tolerance, finally
The result is that promote pearl sac formed, improve pearl production.
Detailed description of the invention
The 6th day control group (left side) and the intracavitary haemocyte of experimental group (right side) pearl sac compare figure after the slotting core of Fig. 1.
Specific embodiment
The present invention is described in further details with specific embodiment with reference to the accompanying drawings of the specification, but embodiment is not right
The present invention limits in any form.Unless stated otherwise, the present invention uses reagent, method and apparatus are normal for the art
Advise reagent, method and apparatus.
Embodiment 1
A method of it reducing receptor shellfish trnasplantion immunity and repels, this method is mould by immunosuppressor ring spore by following steps
Plain A is brought into pteria martensii body: (1) Ciclosporin A being dissolved in basal liquid and be configured to 0.45% Ciclosporin A solution；(2)
Pteria martensii mantle piece is made, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated
In Ciclosporin A solution；(4) according to a conventional method by mantle piece and pearl renucleation pteria martensii visceral mass；(5) spray is utilized
It is spraying that day with fog plants core position progress basal liquid to pteria martensii；(6) it carries out cultivation according to a conventional method to grow cultured pearls, basal liquid is every liter
The seawater that sterilizes dissolves 3 grams of glucose, and 1 gram of compound amino acid, 0.1 gram of Enrofloxacin HCL configures.
Embodiment 2
A method of it reducing receptor shellfish trnasplantion immunity and repels, this method is mould by immunosuppressor ring spore by following steps
Plain A is brought into large nacre body: (1) Ciclosporin A being dissolved in basal liquid and be configured to 0.55% Ciclosporin A solution；(2) it makes
Make big black shell mantle flakes, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated on ring
P0-357 solution A；(4) according to a conventional method by mantle piece and pearl renucleation large nacre；(5) using sprayer to big pearl oyster
It is spraying that shellfish plants core position progress basal liquid；(6) it carries out cultivation according to a conventional method to grow cultured pearls, basal liquid is that every liter of sterilizing seawater dissolves Portugal
5 grams of grape sugar, 3 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL configures.
Embodiment 3
A method of it reducing receptor shellfish trnasplantion immunity and repels, this method is mould by immunosuppressor ring spore by following steps
Plain A is brought into nacre body: (1) Ciclosporin A being dissolved in basal liquid and be configured to 0.50% Ciclosporin A solution；(2) it makes
Ciclosporin A solution is added dropwise and infiltrates on mantle piece by black shell mantle flakes；(3) that pearl core is infiltrated on ring spore is mould
Plain solution A；(4) according to a conventional method by mantle piece and pearl renucleation nacre visceral mass；(5) using sprayer to nacre
It is spraying to plant core position progress basal liquid；(6) it carries out cultivation according to a conventional method to grow cultured pearls, basal liquid is that every liter of sterilizing seawater dissolves grape
4 grams of sugar, 2 grams of compound amino acid, 0.2 gram of Enrofloxacin HCL configures.
Embodiment 4
Time: on May 23rd, 2016 slotting core, on January 7th, 2017 receipts pearl
Place: the village Xuwen County great Jing
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, this method passes through following steps for immune suppression
Preparation Ciclosporin A is brought into pteria martensii body: (1) Ciclosporin A being dissolved in basal liquid, to be configured to 0.50% ring spore mould
Plain solution A；(2) pteria martensii mantle piece is made, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3)
Pearl core is infiltrated on Ciclosporin A solution；(4) according to a conventional method by mantle piece and pearl renucleation pteria martensii internal organ
Group；(5) core position progress basal liquid is planted to pteria martensii using sprayer to be sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls,
Basal liquid is that every liter of sterilizing seawater dissolves 4 grams of glucose, and 2 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL configures.
Experimental design: Ciclosporin A group and each slotting core 2000 of control group (conventional manufacturing techniques), each group is by identical
Small pieces technician and slotting nuclear technology person make mantle piece and slotting core.
Performance of growing cultured pearls compares: Ciclosporin A group survival rate, core-remaining rate and average nacre thickness are respectively increased
10.5%, 14.9%, 8.99%.
1 embodiment of table, 4 each group is grown cultured pearls performance test
Embodiment 5
Time: on April 22nd, 2017 slotting core
Place: the village Xuwen County great Jing
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, this method passes through following steps for immune suppression
Preparation Ciclosporin A is brought into pteria martensii body: (1) Ciclosporin A being dissolved in basal liquid, to be configured to 0.55% ring spore mould
Plain solution A；(2) pteria martensii mantle piece is made, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3)
Pearl core is infiltrated on Ciclosporin A solution；(4) according to a conventional method by mantle piece and pearl renucleation pteria martensii internal organ
Group；(5) core position progress basal liquid is planted to pteria martensii using sprayer to be sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls,
Basal liquid is that every liter of sterilizing seawater dissolves 4 grams of glucose, and 2 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL configures.
Experimental design: Ciclosporin A group and each 100 shellfishes of slotting core of control group replace conventional pearl core with wax core.Each group is equal
Mantle piece and slotting core are made by identical small pieces technician and slotting nuclear technology person.Pearl lens capsule tissue is taken within the 6th day after inserting core, benefit
The intracavitary haemocyte of pearl sac is observed with histotomy and HE decoration method.
As a result: with control group phase, Ciclosporin A processing conspicuousness reduces the quantity of the intracavitary haemocyte of pearl sac, explanation
Ciclosporin A conspicuousness reduces the immunological rejection of slotting core shellfish, and too strong immune response is avoided to generate damage to pearl shell.
Embodiment 6
Time: 23-24 days in October, 2017 slotting core, the receipts pearl on the 5th of September in 2018
Place: the village Xuwen County great Jing
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, this method passes through following steps for immune suppression
Preparation Ciclosporin A is brought into pteria martensii body: (1) Ciclosporin A being dissolved in basal liquid, to be configured to 0.50% ring spore mould
Plain solution A；(2) pteria martensii mantle piece is made, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3)
Pearl core is infiltrated on Ciclosporin A solution；(4) according to a conventional method by mantle piece and pearl renucleation pteria martensii internal organ
Group；(5) core position progress basal liquid is planted to pteria martensii using sprayer to be sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls,
Basal liquid is that every liter of sterilizing seawater dissolves 5 grams of glucose, and 2 grams of compound amino acid, 0.2 gram of Enrofloxacin HCL configures.
Experimental design: Ciclosporin A group inserts core 5000, and control group inserts core 3000 according to conventional manufacturing techniques, each group
Mantle piece and slotting core are made by identical small pieces technician and slotting nuclear technology person.
Performance of growing cultured pearls compares: Ciclosporin A group commodity pearl rate and control group are almost the same, but survival rate, core-remaining rate, excellent pearl
Rate and every ten thousand shellfishes commodity pearl yield have been respectively increased 10.9%, 17.1%, 27.6% and 19.7%.
1 embodiment of table, 6 each group is grown cultured pearls performance test
Embodiment 7
Time: the slotting core on the 19th of August in 2017, the receipts pearl on the 15th of August in 2018
Place: the village Xuwen County Cheng Wu
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, this method passes through following steps for immune suppression
Preparation Ciclosporin A is brought into large nacre body: (1) Ciclosporin A being dissolved in basal liquid and be configured to 0.55% Ciclosporin A
Solution；(2) big black shell mantle flakes are made, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3) by pearl
Core is infiltrated on Ciclosporin A solution；(4) according to a conventional method by mantle piece and pearl renucleation " left bag " (before enteron aisle returns at song
The place of side and paw withdrawal belly of muscle face)；(5) spraying to core position progress basal liquid is planted using sprayer；(6) it carries out according to a conventional method
Cultivation is grown cultured pearls, and basal liquid is that every liter of sterilizing seawater dissolves 5 grams of glucose, and 3 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL is matched
It sets.
Experimental design: Ciclosporin A group and each slotting core 300 of control group (conventional manufacturing techniques), mantle piece and plant
The same technician completes respectively for core operation；It plants core mother shellfish and derives from Sanya cultured population, female long 14-16cm of shell.
Performance of growing cultured pearls compares: Ciclosporin A group survival rate, core-remaining rate, commodity pearl rate and average nacre thickness mention respectively
It is high by 10.1%, 14.5%, 12.7% and 8.6%.
3 embodiment of table, 7 each group is grown cultured pearls performance test
Pass through embodiments above, it can be deduced that conclusion, Ciclosporin A can conspicuousness reduce insert core shellfish immunological rejection
Reaction avoids too strong immune response from generating damage to pearl shell, promotes pearl sac to be formed, to improve pearl production.
Claims (6)
1. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling, it is characterised in that: this method passes through following steps for immune suppression
Preparation Ciclosporin A is brought into receptor shellfish body: (1) Ciclosporin A being dissolved in basal liquid and be configured to Ciclosporin A solution；(2)
Seawater pearl shell mantle piece is made, Ciclosporin A solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated
In Ciclosporin A solution；(4) according to a conventional method by mantle piece and pearl renucleation receptor shellfish visceral mass；(5) sprayer is utilized
Core position is planted to receptor shellfish to be sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls.
2. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: the ring spore is mould
Plain solution A contains the Ciclosporin A of 0.4%-0.55%, and the percentage is volume ratio.
3. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: the basal liquid
3-5 grams of glucose is dissolved with every liter of sterilizing seawater, 1-3 grams of compound amino acid, 0.1-0.2 grams of antibiotic configures.
4. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: described spraying
Solution is basic liquid.
5. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: the receptor shellfish
For pteria martensii, large nacre, nacre or Pteria penguin.
6. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 3, it is characterised in that: the antibiotic
For penicillin+streptomysin, Florfenicol+fortimicin or Enrofloxacin HCL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811164623.7A CN109042413A (en) | 2018-10-06 | 2018-10-06 | A method of it reducing receptor shellfish trnasplantion immunity and repels |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811164623.7A CN109042413A (en) | 2018-10-06 | 2018-10-06 | A method of it reducing receptor shellfish trnasplantion immunity and repels |
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| CN109042413A true CN109042413A (en) | 2018-12-21 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007110902A (en) * | 2005-09-21 | 2007-05-10 | Sannoki Kazutaka | Method for producing seed and seedling of pearl oyster |
| CN103598129A (en) * | 2013-12-02 | 2014-02-26 | 浙江海洋学院 | Method for cultivating iridescence fresh water pearls |
| CN107613764A (en) * | 2015-05-19 | 2018-01-19 | 安托法加斯塔大学 | The method that Wild pearl is produced by abalone |
-
2018
- 2018-10-06 CN CN201811164623.7A patent/CN109042413A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007110902A (en) * | 2005-09-21 | 2007-05-10 | Sannoki Kazutaka | Method for producing seed and seedling of pearl oyster |
| CN103598129A (en) * | 2013-12-02 | 2014-02-26 | 浙江海洋学院 | Method for cultivating iridescence fresh water pearls |
| CN107613764A (en) * | 2015-05-19 | 2018-01-19 | 安托法加斯塔大学 | The method that Wild pearl is produced by abalone |
Non-Patent Citations (2)
| Title |
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| 戈贤平: "《淡水珍珠养殖新技术》", 31 August 2002, 上海科学技术出版社 * |
| 王梅芳: "育珠贝细胞小片及处理技术研究进展", 《广东海洋大学学报》 * |
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