CN109169443A - A method of it reducing receptor shellfish trnasplantion immunity and repels - Google Patents
A method of it reducing receptor shellfish trnasplantion immunity and repels Download PDFInfo
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- CN109169443A CN109169443A CN201811164622.2A CN201811164622A CN109169443A CN 109169443 A CN109169443 A CN 109169443A CN 201811164622 A CN201811164622 A CN 201811164622A CN 109169443 A CN109169443 A CN 109169443A
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- mycophenolate
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- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000036039 immunity Effects 0.000 title claims abstract description 23
- HPNSFSBZBAHARI-RUDMXATFSA-N mycophenolic acid Chemical compound OC1=C(C\C=C(/C)CCC(O)=O)C(OC)=C(C)C2=C1C(=O)OC2 HPNSFSBZBAHARI-RUDMXATFSA-N 0.000 claims abstract description 67
- HPNSFSBZBAHARI-UHFFFAOYSA-N micophenolic acid Natural products OC1=C(CC=C(C)CCC(O)=O)C(OC)=C(C)C2=C1C(=O)OC2 HPNSFSBZBAHARI-UHFFFAOYSA-N 0.000 claims abstract description 64
- 239000011049 pearl Substances 0.000 claims abstract description 62
- 229940014456 mycophenolate Drugs 0.000 claims abstract description 58
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000007796 conventional method Methods 0.000 claims abstract description 20
- 239000010984 cultured pearl Substances 0.000 claims abstract description 18
- 239000013535 sea water Substances 0.000 claims abstract description 15
- 239000003018 immunosuppressive agent Substances 0.000 claims abstract description 13
- 230000009278 visceral effect Effects 0.000 claims abstract description 10
- -1 Phenols acids Chemical class 0.000 claims description 15
- 241000879903 Pteria Species 0.000 claims description 14
- 238000005507 spraying Methods 0.000 claims description 12
- PZJWYUDBXNNVLZ-UHFFFAOYSA-N 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxoquinoline-3-carboxylic acid;hydrochloride Chemical compound Cl.C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 PZJWYUDBXNNVLZ-UHFFFAOYSA-N 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 230000003115 biocidal effect Effects 0.000 claims description 4
- 229960000951 mycophenolic acid Drugs 0.000 claims description 4
- AYIRNRDRBQJXIF-NXEZZACHSA-N (-)-Florfenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CF)NC(=O)C(Cl)Cl)C=C1 AYIRNRDRBQJXIF-NXEZZACHSA-N 0.000 claims description 2
- 229930195503 Fortimicin Natural products 0.000 claims description 2
- 229930182555 Penicillin Natural products 0.000 claims description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 2
- 241000183300 Pteria penguin Species 0.000 claims description 2
- BIDUPMYXGFNAEJ-APGVDKLISA-N astromicin Chemical compound O[C@@H]1[C@H](N(C)C(=O)CN)[C@@H](OC)[C@@H](O)[C@H](N)[C@H]1O[C@@H]1[C@H](N)CC[C@@H]([C@H](C)N)O1 BIDUPMYXGFNAEJ-APGVDKLISA-N 0.000 claims description 2
- 229960003760 florfenicol Drugs 0.000 claims description 2
- 229940049954 penicillin Drugs 0.000 claims description 2
- 230000001846 repelling effect Effects 0.000 claims 6
- 238000004519 manufacturing process Methods 0.000 abstract description 12
- 230000001900 immune effect Effects 0.000 abstract description 5
- 230000006058 immune tolerance Effects 0.000 abstract description 5
- 230000009467 reduction Effects 0.000 abstract description 3
- 230000002980 postoperative effect Effects 0.000 abstract description 2
- 230000014509 gene expression Effects 0.000 description 6
- RTGDFNSFWBGLEC-SYZQJQIISA-N mycophenolate mofetil Chemical compound COC1=C(C)C=2COC(=O)C=2C(O)=C1C\C=C(/C)CCC(=O)OCCN1CCOCC1 RTGDFNSFWBGLEC-SYZQJQIISA-N 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 238000013401 experimental design Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 238000005025 nuclear technology Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 238000011056 performance test Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 108010057466 NF-kappa B Proteins 0.000 description 2
- 102000003945 NF-kappa B Human genes 0.000 description 2
- 102000001253 Protein Kinase Human genes 0.000 description 2
- 206010052779 Transplant rejections Diseases 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 206010020718 hyperplasia Diseases 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229960004866 mycophenolate mofetil Drugs 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- VXQOFFOCENPBFI-MCDZGGTQSA-N (2r,3r,4s,5r)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol;diphosphono hydrogen phosphate Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O.C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O VXQOFFOCENPBFI-MCDZGGTQSA-N 0.000 description 1
- 235000013411 Alpinia speciosa Nutrition 0.000 description 1
- 244000060696 Alpinia speciosa Species 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 108010014632 NF-kappa B kinase Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000490567 Pinctada Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
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- 239000011324 bead Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 description 1
- 235000013928 guanylic acid Nutrition 0.000 description 1
- 239000004226 guanylic acid Substances 0.000 description 1
- 230000008629 immune suppression Effects 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
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- 238000002054 transplantation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
- A01K61/56—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of methods that reduction receptor shellfish trnasplantion immunity is repelled, belong to seawater pearl culture technical field, immunosuppressor mycophenolate is brought into receptor shellfish body by following steps: (1) mycophenolate being dissolved in basal liquid and is configured to mycophenolate solution；(2) seawater pearl shell mantle piece is made, mycophenolate solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated on mycophenolate solution；(4) according to a conventional method by mantle piece and pearl renucleation receptor shellfish visceral mass；(5) core position is planted to receptor shellfish using sprayer to be sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls；Immunosuppressor mycophenolate is applied to production of growing cultured pearls by this method, appropriate to reduce receptor shellfish in the plant postoperative trnasplantion immunity rejection of core, is accelerated receptor shellfish and is converted from immunological rejection to immune tolerance, promotes pearl sac to be formed, to improve pearl production.
Description
Technical field
The present invention relates to a kind of methods that reduction receptor shellfish trnasplantion immunity is repelled more particularly to a kind of utilization mycophenolate to drop
The method that low receptor shellfish trnasplantion immunity is repelled, belongs to seawater pearl culture technical field.
Background technique
Artificial culture sea water pearls are the forming process for imitating natural peral, by performing the operation the mantle piece of donor shellfish
Tissue and a circular pearl core are transplanted to together in the visceral mass connective tissue of receptor shellfish.Then, mantle piece tissue with by
Body shellfish tissue interaction, epithelial cell are gradually proliferated, break up, and form the pearl sac for surrounding pearl core.Pearl sac constantly secretes treasure
Pearl matter is deposited on pearl core surface and forms pearl.In production, benefit of growing cultured pearls usually is improved using mantle piece Precerving liquid, it is former
Reason mainly maintains small pieces activity, promotes cell Proliferation and avoids infection.
The implantation process of mantle piece and pearl core is similar to the organ transplant of higher organism, after the transfer initial stage, implantation
Allosome mantle tissue and pearl core can cause the graft-rejection of receptor shellfish, and about 10-15 days after the transfer, a part of receptor shellfish
The small pieces tissue and pearl core for gradually receiving implantation establish immune tolerance, form the pearl sac for surrounding pearl core.In this process, mistake
Strong rejection will lead to the core of spitting of pearl sac dysplasia and receptor shellfish, and even receptor shellfish is dead, to reduce treasure
The quality and yield of pearl.
Therefore, Effective Regulation pearl shell plants the graft rejection after core, facilitates the immune tolerance of pearl shell, is that pearl sector is held
Problem in the urgent need to address in supervention exhibition, and the not yet targeted scheme of existing production technology.
Immunosuppressor is a kind of drug that can inhibit the reaction of body abnormal immune, is clinically mainly used for preventer
Rejection when official transplants.Mycophenolate (Mycophenolate Mofetil, MMF) also known as mycophenolate mofetil, are one
Kind small toxicity, the strong immunosuppressor of immunosuppressive action have been widely used in clinical filed of organ transplantation at present, have made organ
The success rate of transplanting greatly improves.Studies have shown that MMF is first converted into active metabolite mycophenolic acid in vivo
(mycophenolic acid, MPA).MPA reversibly inhibits the speed limit of guanopterin nucleoside triphosphate adenosine de novo synthesis
Enzyme-carnine acidohydrogenase, to reduce intracellular guanylic acid, the synthesis of blocking dna inhibits immune thin
The hyperplasia of born of the same parents.Meanwhile MMF can also inhibit immunocyte to inflammation by the formation of inhibition immunocyte surface adhesion molecule
The aggregation at position.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of methods that reduction receptor shellfish trnasplantion immunity is repelled, and pass through
Mantle piece and pearl core bring mycophenolate solution in receptor shellfish body into, the inhibition being immunoreacted using mycophenolate to receptor shellfish
Effect accelerates receptor shellfish from immunological rejection to immune tolerance and converts the survival of promotion mantle piece and hyperplasia, to promote treasure
Pearl capsule is formed, and core-remaining rate and bead yield rate is improved, to improve pearl production.
Above-mentioned purpose of the invention is achieved by the following technical programs:
A method of it reducing receptor shellfish trnasplantion immunity and repels, brought into immunosuppressor mycophenolate by following steps
In receptor shellfish body: (1) mycophenolate being dissolved in basal liquid and be configured to mycophenolate solution；(2) seawater pearl shell housing is made
Mycophenolate solution is added dropwise and infiltrates on mantle piece by film small pieces；(3) pearl core is infiltrated on mycophenolate solution；(4) it presses
Conventional method is by mantle piece and pearl renucleation receptor shellfish visceral mass；(5) core position is planted to receptor shellfish using sprayer to carry out
It is spraying；(6) cultivation is carried out according to a conventional method to grow cultured pearls；
Above-mentioned mycophenolate solution contains the mycophenolate of 0.20%-0.35%；Above-mentioned basal liquid is with every liter of sterilizing seawater
3-5 grams of glucose of dissolution, 1-3 grams of compound amino acid, 0.1-0.15 grams of antibiotic configures；Based on above-mentioned spraying solution
Liquid；Above-mentioned receptor shellfish is pteria martensii, large nacre, nacre or Pteria penguin；Above-mentioned antibiotic is penicillin+strepto-
Element, Florfenicol+fortimicin or Enrofloxacin HCL.
The invention has the advantages that immunosuppressor mycophenolate is applied to production of growing cultured pearls by this method, it is appropriate to reduce
Receptor shellfish is planting the postoperative trnasplantion immunity rejection of core, accelerates receptor shellfish and converts from immunological rejection to immune tolerance, promotes
Pearl sac is formed, to improve pearl production.
Detailed description of the invention
Fig. 1 is that mycophenolate group and control group pearly shellfish blood cell immunity related gene expression amount compare；It (a) is mycophenolic acid
Ester processing inhibits the influence of protein kinase (inhibitor of NF- κ B kinases, IKK) gene expression to Nuclear factor kappa B,
(b) influence to Nuclear factor kappa B (Nuclearfactor kB, NF- κ B) gene expression is handled for mycophenolate.
Specific embodiment
The present invention is described in further details with specific embodiment with reference to the accompanying drawings of the specification, but embodiment is not right
The present invention limits in any form.Unless stated otherwise, the present invention uses reagent, method and apparatus are normal for the art
Advise reagent, method and apparatus.
Embodiment 1
A method of it reducing receptor shellfish trnasplantion immunity and repels, brought into immunosuppressor mycophenolate by following steps
In pteria martensii body: (1) mycophenolate being dissolved in basal liquid and be configured to 0.20% mycophenolate solution；(2) geneva is made
Mycophenolate solution is added dropwise and infiltrates on mantle piece by black shell mantle flakes；(3) pearl core is infiltrated on mycophenolate
Solution；(4) according to a conventional method by mantle piece and pearl renucleation pteria martensii visceral mass；(5) using sprayer to geneva
It is spraying that nacre plants core position progress basal liquid；(6) cultivation is carried out according to a conventional method to grow cultured pearls；The basal liquid is sterilized with every liter
Seawater dissolves 3 grams of glucose, and 1 gram of compound amino acid, 0.1 gram of Enrofloxacin HCL configures.
Embodiment 2
A method of it reducing receptor shellfish trnasplantion immunity and repels, brought into immunosuppressor mycophenolate by following steps
In large nacre body: (1) mycophenolate being dissolved in basal liquid and be configured to 0.35% mycophenolate solution；(2) big pearl oyster is made
Mycophenolate solution is added dropwise and infiltrates on mantle piece by shellfish mantle piece；(3) that pearl core is infiltrated on mycophenolate is molten
Liquid；(4) according to a conventional method by mantle piece and pearl renucleation large nacre visceral mass；(5) using sprayer to large nacre
It is spraying to plant core position progress basal liquid；(6) cultivation is carried out according to a conventional method to grow cultured pearls；The basal liquid is molten with every liter of sterilizing seawater
5 grams of glucose of solution, 3 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL configures.
Embodiment 3
A method of it reducing receptor shellfish trnasplantion immunity and repels, brought into immunosuppressor mycophenolate by following steps
In nacre body: (1) mycophenolate being dissolved in basal liquid and be configured to 0.3% mycophenolate solution；(2) nacre housing is made
Mycophenolate solution is added dropwise and infiltrates on mantle piece by film small pieces；(3) pearl core is infiltrated on mycophenolate solution；(4) it presses
Conventional method is by mantle piece and pearl renucleation nacre visceral mass；(5) core position is planted to nacre using sprayer to carry out
Basal liquid is spraying；(6) cultivation is carried out according to a conventional method to grow cultured pearls；The basal liquid dissolves 4 grams of glucose with every liter of sterilizing seawater,
2 grams of compound amino acid, 0.2 gram of Enrofloxacin HCL configures.
Embodiment 4
Time: on May 25th, 2016 slotting core, on January 7th, 2017 receipts pearl
Place: the village Xuwen County great Jing
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, it is by following steps that immunosuppressor is mould
Phenols acids are brought into pteria martensii body: (1) mycophenolate being dissolved in basal liquid and configure 0.25% mycophenolate solution；(2)
Pteria martensii mantle piece is made, mycophenolate solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated on
Mycophenolate solution；(4) according to a conventional method by mantle piece and pearl renucleation pteria martensii visceral mass；(5) using spraying
It is spraying that device plants core position progress basal liquid to pteria martensii；(6) cultivation is carried out according to a conventional method to grow cultured pearls；Basal liquid is gone out with every liter
Bacterium seawater dissolves 4 grams of glucose, and 2 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL configures.
Experimental design: mycophenolate group and each slotting core 2000 of control group (conventional manufacturing techniques), each group is by identical
Small pieces technician and slotting nuclear technology person make mantle piece and slotting core.
Performance of growing cultured pearls compares: mycophenolate group survival rate, core-remaining rate and average nacre thickness has been respectively increased 7.9%,
24.5%, 8.0%.
1 embodiment of table, 4 each group is grown cultured pearls performance test
Embodiment 5
Time: on April 22nd, 2017 slotting core, 6h, 12h, 1d after inserting core, 3d, 6d, 12d, 18d, 30d is sampled.
Place: the village Xuwen County great Jing
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, it is by following steps that immunosuppressor is mould
Phenols acids are brought into pteria martensii body: (1) mycophenolate being dissolved in basal liquid and configure 0.30% mycophenolate solution；(2)
Pteria martensii mantle piece is made, mycophenolate solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated on
Mycophenolate solution；(4) according to a conventional method by mantle piece and pearl renucleation pteria martensii visceral mass；(5) using spraying
It is spraying that device plants core position progress basal liquid to pteria martensii；(6) cultivation is carried out according to a conventional method to grow cultured pearls；Basal liquid is gone out with every liter
Bacterium seawater dissolves 4 grams of glucose, and 2 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL configures.
Experimental design: mycophenolate group inserts core 100, and control group inserts core 100 according to conventional manufacturing techniques, and each group is by phase
Same small pieces technician and slotting nuclear technology person make mantle piece and slotting core.Take pearly shellfish blood thin respectively at above-mentioned each time point
Born of the same parents extract RNA, utilize the expression of method detection the gene involved in immunity IKK and NF-kb of quantitative fluorescent PCR.
As a result: as shown in Figure 1, compared with the control group, 3d days to 12 days after inserting core, mycophenolate handles conspicuousness and reduces
The expression of IKK and NF- κ b.IKK and NF- κ b is the key component in NF- κ b signal path, and expression, which reduces, illustrates mould phenol
Acid esters processing can conspicuousness reduce insert core shellfish immunological rejection, avoid too strong immune response to pearl shell generate damage.
Significant difference (P < 0.05) is shown with colonnade subscript difference lowercase alphabet.
Embodiment 6
Time: 15-16 days in November, 2017 slotting core, the receipts pearl on the 18th of September in 2018
Place: the village Xuwen County great Jing
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, it is by following steps that immunosuppressor is mould
Phenols acids are brought into pteria martensii body: (1) mycophenolate being dissolved in basal liquid and configure 0.30% mycophenolate solution；(2)
Pteria martensii mantle piece is made, mycophenolate solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is infiltrated on
Mycophenolate solution；(4) according to a conventional method by mantle piece and pearl renucleation nacre visceral mass；(5) sprayer pair is utilized
It is spraying that nacre plants core position progress basal liquid；(6) cultivation is carried out according to a conventional method to grow cultured pearls；Basal liquid is molten with every liter of sterilizing seawater
5 grams of glucose of solution, 2 grams of compound amino acid, 0.2 gram of Enrofloxacin HCL configures.
Experimental design: mycophenolate group inserts core 5000, and control group inserts core 3000 according to conventional manufacturing techniques, and each group is equal
Mantle piece and slotting core are made by identical small pieces technician and slotting nuclear technology person.
Performance of growing cultured pearls compares: mycophenolate group survival rate and commodity pearl rate and control group are almost the same, but core-remaining rate improves
27.5%, excellent pearl rate improves 17.8%, every ten thousand shellfishes commodity pearl output increased 23.2%.
2 embodiment of table, 6 each group is grown cultured pearls performance test
Embodiment 7
Time: the slotting core on the 25th of August in 2017, the receipts pearl on the 15th of August in 2018
Place: the village Xuwen County Cheng Wu
Experimental method: a method of it reducing receptor shellfish trnasplantion immunity and repels, this method passes through following steps for immune suppression
Preparation mycophenolate is brought into large nacre body: (1) mycophenolate being dissolved in basal liquid, to be configured to 0.35% mycophenolate molten
Liquid；(2) big black shell mantle flakes are made, mycophenolate solution is added dropwise and is infiltrated on mantle piece；(3) pearl core is soaked
Moisten in mycophenolate solution；(4) by mantle piece and pearl renucleation " left bag ", (enteron aisle returns front and contracting at song according to a conventional method
The place in sufficient belly of muscle face)；(5) spraying to core position progress basal liquid is planted using sprayer；(6) cultivation is carried out according to a conventional method to educate
Pearl, basal liquid are that every liter of sterilizing seawater dissolves 5 grams of glucose, 3 grams of compound amino acid, 0.15 gram of Enrofloxacin HCL configure and
At.
Experimental design: mycophenolate group and each slotting core 300 of control group (conventional manufacturing techniques), mantle piece and plant core
The same technician completes respectively for operation；It plants core mother shellfish and derives from Sanya cultured population, female long 14-16cm of shell.
Performance of growing cultured pearls compares: mycophenolate group survival rate, core-remaining rate, commodity pearl rate and average nacre thickness are respectively increased
10.7%, 18.5%, 13.2% and 12.1%.
3 embodiment of table, 7 each group is grown cultured pearls performance test
Pass through embodiments above, it can be deduced that conclusion, mycophenolate can conspicuousness reduce insert core shellfish immunological rejection
Reaction avoids too strong immune response from generating damage to pearl shell, promotes pearl sac to be formed, to improve pearl production.
Claims (6)
1. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling, it is characterised in that: by following steps that immunosuppressor is mould
Phenols acids are brought into receptor shellfish body: (1) mycophenolate being dissolved in basal liquid and be configured to mycophenolate solution；(2) seawater is made
Mycophenolate solution is added dropwise and infiltrates on mantle piece by pearl shell mantle small pieces；(3) pearl core is infiltrated on mycophenolate
Solution；(4) according to a conventional method by mantle piece and pearl renucleation receptor shellfish visceral mass；(5) receptor shellfish is planted using sprayer
Core position is sprayed；(6) cultivation is carried out according to a conventional method to grow cultured pearls.
2. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: the mycophenolic acid
Ester solution contains the mycophenolate of 0.20%-0.35%, and the percentage is volume ratio.
3. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: the basal liquid
3-5 grams of glucose is dissolved with every liter of sterilizing seawater, 1-3 grams of compound amino acid, 0.1-0.2 grams of antibiotic configures.
4. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: described spraying
Solution is basic liquid.
5. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 1, it is characterised in that: the receptor shellfish
For pteria martensii, large nacre, nacre or Pteria penguin.
6. a kind of method for reducing receptor shellfish trnasplantion immunity and repelling according to claim 3, it is characterised in that: the antibiotic
For penicillin+streptomysin, Florfenicol+fortimicin or Enrofloxacin HCL.
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| CN113711960A (en) * | 2021-09-30 | 2021-11-30 | 北部湾大学 | Method for reducing seawater pearl shell heterograft immune rejection |
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