CN109021090A - 第2209位点发生突变的人类ibgc致病基因pdgfrb及其检测方法 - Google Patents
第2209位点发生突变的人类ibgc致病基因pdgfrb及其检测方法 Download PDFInfo
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Abstract
本发明涉及第2209位点发生突变的人类IBGC致病基因PDGFB，其核苷酸序列如SEQ ID NO.3所示，具体是野生型基因第2209位的G突变为A。本发明提供的致病基因形式尚未被报道，可以为该病致病机理的解析和药物开发、致病基因筛查和检测以及治疗方案的制定等提供依据和奠定基础。同时本发明构建了致病基因检测方法：首先利用多重PCR捕获致病基因外显子区域，再对其进行二代测序、通过信息分析找出突变，最后利用Sanger测序对突变进行验证；其中PCR捕获引物组包含扩增引物序列SEQ ID NO.12‑23，Sanger测序片段的扩增引物序列如SEQ ID NO.28‑29所示。本发明检测方法可以高效全面、快速准确地获取突变信息。
Description
本案是专利申请No.201710071474.9(申请日为：2017年2月9日，发明名称为：人类特发性基底节钙化致病基因及其检测方法)的分案申请。
技术领域
本发明涉及基因及其检测方法，特别涉及第2209位点发生突变的人类IBGC致病基因PDGFRB及其检测方法。
背景技术
人类特发性基底节钙化(Idiopathic Basal Ganglia Calcification,IBGC)是一种以影像学上基底节及脑其它部位对称性钙化为特征的神经系统变性遗传病,也称Fahr病。钙化最常见的部位为基底节区豆状核，尤其是苍白球，也常见于壳核、丘脑、尾状核及小脑齿状核等部位，甚至出现在大脑皮层及皮层下白质。
IBGC属罕见病，呈散发或家族性发病，家族性患者发病年龄逐代提前，进展缓慢、病程较长。该病无明显性别差异，表现为复杂多样的运动损害和行为症状的组合，同一家族中的不同成员之间临床症状往往存在差异。临床表现以神经、精神功能紊乱为主，轻者仅仅为轻度注意力或记忆力的下降，重者则会出现人格及行为改变，终致精神病或痴呆。
IBGC有家族性发病的现象，常染色体显性遗传和隐性遗传的病例均有报道，以常染色体显性遗传居多。遗传性IBGC相关致病基因和发病机制长期以来并未得到解析，直到2012年Liu等人在IBGC3基因位点中，通过连锁分析、单倍体分析、Sanger测序等成功克隆特发性基底节钙化的致病基因SLC20A2，并且在不同种族中得到验证；此后，PDGFRB、PDGFB和XPR1这三个致病基因陆续被发现和得到验证。其中，SLC20A2基因编码的蛋白为钠-磷共转运体(PiT2)，参与机体的钙磷调节；PDGFRB基因编码血小板衍生生长因子受体PDGFRβ，属于III型受体酪氨酸激酶家族；PDGFB基因则编码血小板衍生生长因子PDGF-B，与PDGFBβ结合可激活下游信号通路，参与细胞的增殖、分化、生存以及迁移；XPR1基因编码的蛋白含有SPX结构域，主要参与磷稳态的维持，具有Pi输出功能。
为了系统解析IBGC致病基因突变，本发明设计和合成了覆盖4个已知致病基因的PCR捕获引物组，并结合二代测序技术对10个IBGC患者样本进行捕获测序，共分析得出了1种SLC20A2基因突变、2种PDGFRB基因突变、2种PDGFB基因突变和2种XPR1基因突变，所发现的突变均经Sanger测序确证；之后针对各自的致病基因突变，利用Sanger测序分析对应的家系中其它成员的样本，全部符合基因型-表型共分离规律从而致病基因突变得到了验证。进一步的，采用同样的方法在另外12个IBGC患者和200个正常人样本中进行了验证。本发明提供的IBGC致病基因突变尚未见报道，其对于该病的解析、筛查、检测和治疗具有潜在的重要价值。此外，本发明基于PCR捕获结合二代测序(简称PCR捕获测序)构建的IBGC致病基因检测方法，可以同时全面解析4种IBGC致病基因突变谱，具有通量高、灵敏度高、可解析未知突变的优点。目前，国内外均没有该方法在IBGC致病基因SLC20A2，PDGFRB，PDGFB，XPR1突变同时检测方面应用的报道。由于单次反应测序长度的限制(1000bp以内)，传统的基于Sanger测序的方法进行的全面解析，费用高昂且费时费力。而基于实时荧光定量PCR的突变检测方法，则只能针对少量已知的突变。
发明内容
本发明提供了IBGC相关的4个致病基因的7种突变基因形式，同时提供了基于PCR捕获测序的检测方法；这些可以为IBGC致病机理的解析和药物开发、致病基因筛查和检测以及治疗方案的制定等提供依据和奠定基础。
本发明解决其技术问题所采用的技术方案如下：
本发明提供了IBGC的7种致病基因形式，包括1种SLC20A2突变基因，其序列如SEQID NO.1所示；2种PDGFRB突变基因，其序列如SEQ ID NO.2和SEQ ID NO.3所示；2种PDGFB突变基因，其序列如SEQ ID NO.4和SEQ ID NO.5所示；和2种XPR1突变基因，其序列如SEQ IDNO.6和SEQ ID NO.7所示。
所述涉及SLC20A2、PDGFRB、PDGFB和XPR1这4个基因共7种突变基因类型经检测和验证，均为IBGC致病基因。其中，SLC20A2突变基因的突变位点在Exon7中，具体是野生型基因第799位的G突变为C；PDGFRB突变基因的突变位点其一在Exon 2中，具体是野生型基因第3位的G突变为A,其二在Exon16中，具体是野生型基因第2209位的G突变为A；PDGFB突变基因的突变位点其一在Exon3中，具体是野生型基因第220位的G突变为T，其二也在Exon3中，具体是野生型基因第232位的C突变为T；XPR1突变基因的突变位点其一在Exon 5中，具体是野生型基因第490位的G突变为T，其二在Exon13中，具体是野生型基因第1708位的C突变为T。由于突变发生在外显子区，因此本发明提供的SEQ ID NO.1-7为编码区序列。另外，提供的SEQ ID NO.8-11分别是SLC20A2，PDGFRB，PDGFB，XPR1的野生型基因的编码区序列，供参比。
本发明提供的基于PCR捕获测序的IBGC致病基因检测方法(参见图1)包括如下主要步骤：
(1)4个致病基因的PCR捕获
提取待测样本基因组DNA，利用设计合成的PCR捕获引物组进行扩增；
(2)二代测序解析基因突变谱
将(1)中的多重PCR扩增产物进行二代测序，并通过信息分析找出突变位点；
(3)突变位点的验证
对于结果是已知突变的，则将原样本突变片段扩增后直接进行Sanger测序验证；对于结果是新发现突变的，则根据情况设计引物将原样本突变片段扩增后直接进行Sanger测序验证后，再通过Sanger测序分析患者家系相关成员样本的基因型-表型共分离情况进行验证。
其中，(1)中所述PCR捕获引物组是利用美国Thermo Fisher公司的在线软件IonAmpliSeq Designer进行设计的，至少应该捕获所述所有7种突变形式其突变位点所在区域，结果显示至少需要SEQ ID NO.12-23这12条序列。优选地，可以针对SLC20A2、PDGFRB、PDGFB和XPR1这4个基因所有外显子及其侧翼序列，使得目标基因覆盖率达100％，结果共获得了69对引物，其序列如表1所示。
表1 PCR捕获扩增引物组
其中，(1)中进行的多重PCR扩增体系(20ul)如表2所示。5X Ion AmpliSeqTMHiFiMix、2X Ion AmpliSeqTM引物组和20X Ion AmpliSeqTMSample ID Panel均购置于美国Thermo Fisher公司。
表2 PCR捕获扩增体系
| 成分 | 添加量(ul) |
| 5X Ion AmpliSeqTMHiFi Mix | 4 |
| 2X Ion AmpliSeqTM引物组 | 10 |
| 20X Ion AmpliSeqTMSample ID Panel | 1 |
| 浓度>20ng/ul的样本基因组DNA | 0.5 |
| 灭菌的Nuclease-free Water | 4.5 |
其中，(1)中所述的多重PCR扩增阶段和条件如表3所示：
表3 PCR捕获扩增阶段和条件
其中，(2)中所述的PCR扩增捕获产物进行的二代测序和信息分析，有二代测序仪和配套分析软件则按照规范操作和分析进行，或可由商业化的高通量测序公司完成，最终需得到所检测样本的致病基因突变信息。
其中，(3)中所述的已知突变指的是前述的7种突变形式(可详见表4)，所述新发现突变指的是这7种已发现突变外的其它突变形式。所述的原样本是指(1)中提取的待测样本基因组，除用于PCR捕获测序模板外剩余的部分；或者原检测样本再次提取的基因组DNA。所述突变包括点突变、插入或缺失，但不限于此。
其中，(3)中所述对于已知突变则将原样本突变片段扩增后直接进行Sanger测序验证，是指利用特定的引物对扩增突变片段并进行Sanger测序，验证是否同二代测序检出的突变结果一致；有Sanger测序仪则按规范操作进行测序或可送商业化测序公司完成，最终需得到对应的扩增子序列。所述原样本突变片段扩增是指，对于SLC20A2基因的c.799G>C突变，扩增引物对为SEQ ID NO.24-25；对于PDGFRB基因的c.3G>A和c.2209G>A突变，扩增引物对分别为SEQ ID NO.26-27和SEQ ID NO.28-29；对于PDGFB基因的c.220G>T和c.232C>T突变，扩增引物对分别为SEQ ID NO.28-29和SEQ ID NO.30-31；对于XPR1基因的c.490G>T和c.1708C>T突变，扩增引物对分别为SEQ ID NO.32-33和SEQ ID NO.34-35。
其中，(3)中所述的患者家系相关成员必须包括IBGC患者和非IBGC患者。所述基因型-表型共分离是指基因型决定表型，由此两者符合共同对应的遗传学传递现象和规律。
本发明提供的IBGC相关4个致病基因的7种突变基因类型，可以为该病致病机理的解析和药物开发、致病基因筛查和检测以及治疗方案的制定等提供依据和奠定基础；此外本发明提供的基于PCR捕获测序的致病基因检测方法，覆盖了4个致病基因的全部外显子，可以高效全面、快速准确地获取突变信息。
附图说明
图1是本发明IBGC致病基因检测方法流程图。
图2-8是各种基因突变形式的Sanger测序验证图谱突变位点局部。其中图2对应于实施例1中的1号患者，为SLC20A2基因的c.799G>C突变，2号、3号和4号患者的图谱与其类似；图3、图4、图5、图6、图7、和图8分别对应于实施例1中的5、6、7、8、9和10号患者，分别为PDGFRB的c.3G>A、PDGFRB的c.2209G>A、PDGFB的c.220G>T、PDGFB的c.232C>T、XPR1的c.490G>T和XPR1的c.1708C>T；各图中的序列位点数字只代表对应扩增子，并不代表突变位点在实际基因中的位置。
图9是实施例1中1号IBGC患者颅脑CT平扫影像。
图10是实施例1中1号IBGC患者所在家系的遗传图谱。此图中正方形表示男性，圆形表示女性；箭头表示先证者，带斜线表示死亡，带问号表示未行颅脑CT检查个体；实心表示IBGC患病个体，空心表示正常个体；“+”表示SLC20A2基因检出c.799G>C突变，“-”表示SLC20A2基因未检出c.799G>C突变；I、II和III分别代表第1、2和3代，数字为成员编号。
具体实施方式
以下结合具体实施例，进一步阐述本发明。
实施例1
根据详细的病史和家族史调查情况以及规范的神经系统体格检查和相关辅助检查信息，选择所在医院10名IBGC患者在其签署了知情同意书的情况下，进行致病基因检测和分析。
(1)4个致病基因的PCR捕获
取患者肘静脉血10ml(EDTA抗凝)，用QIAamp DNA Blood Mini Kit(德国Qiagen公司)提取基因组DNA，以其为模板采用表1的PCR捕获引物组按照表2配制扩增体系，并在常规PCR仪上按照表3条件进行反应。
(2)二代测序解析基因突变谱
将(1)中的多重PCR扩增产物送上海晶能生物技术有限公司进行二代测序，采用的是Ion PGM Template OT2 200试剂盒进行乳液PCR，Dynabeads MyOne Streptavidin C1磁珠进行阳性模板富集；以上两步反应均在Ion One Touch系统上进行。建库后采用Ion PGMSequencing kit及Ion Torrent 316 v2芯片，在PGM测序仪上进行测序反应。采用PGM服务器自带分析软件对测序结果初步分析，再用千人基因组数据库、SNP数据库和COSMIC(version 64)数据库等进行突变位点解析，结果如表4所示，共计有7种突变。
表4 10例IBGC患者致病基因检测及验证结果
(3)突变位点的验证
根据所检出的突变，利用前述特定引物对将原样本突变片段扩增后直接进行Sanger测序验证。采用Takara的rTaq聚合酶扩增，其扩增体系详见表5，扩增引物对详见表6，PCR产物送上海生工生物工程股份有限公司进行Sanger测序。Sanger测序结果发现均与二代测序结果一致(表4)。
表5扩增体系
| 编号 | 组份 | 添加量 |
| 1 | 10X PCR buffer(Mg+) | 2.5ul |
| 2 | dNTP Mix | 0.5ul |
| 3 | Primer(50uM)F | 0.25ul |
| 4 | Primer(50uM)R | 0.25ul |
| 5 | rTaq | 0.25ul |
| 6 | Template DNA(30ng/ul) | 1ul |
| 7 | 灭菌ddH2O | 20ul |
表6扩增引物对
扩增条件为：94℃预变性3min；30个循环：94℃变性30s，60℃/65℃退火30s，72℃延伸30s；72℃终延伸5min。
由于这7种突变均未被报道，因此收集这10例IBGC患者家系相关成员样本，再通过Sanger测序突变片段。以S1号患者为例，如图9所示其为家系先证者III4，无明显症状，颅脑CT平扫示双侧苍白球及小脑齿状核对称性大面积钙化。根据PGM数据可知，其SLC20A2基因第799位的G突变为C；利用引物对SEQ ID NO.24-25扩增SLC20A2基因c.799G>C突变片段，其直接Sanger测序结果(如图2所示)，与二代测序结果一致。收集该家系中的其他成员资料，能获得该家系6个阳性成员，4个阴性成员的影像资料(如图10中所示)：即先证者III4、II1、II3、II8、III2及III7的颅脑CT平扫均显示双侧基底节区及小脑存在大片钙化灶，II2、III1、III5、III6颅内均无钙化灶。采集这10个家系成员的血液样本提取基因组DNA，利用引物对SEQ ID NO.24-25扩增SLC20A2基因c.799G>C突变片段，Sanger测序显示6个阳性患者均存在该突变位点，而4个阴性成员均无该位点突变，即出现了基因型-表型的家系共分离现象，由此证明了SLC20A2基因的c.799G>C突变是该家系的致病性突变。表4中其余9例患者，经过同样的分析均出现家系共分离现象(表4)。因此，通过本实施例获得了7种IBGC的致病基因，其序列对应信息详见表4。
(4)致病基因制备和获取
基于本发明新鉴定的IBGC的7种致病基因，为了开展致病机理机制、筛查检测方法和试剂盒开发、治疗药物研发等科研生产工作需要，申请人将突变所在的扩增子进行纯化，将其克隆到商业化质粒等载体中，之后将其导入大肠杆菌等宿主菌中，置于-80℃冰箱中保存。另一方面，由于本发明对于致病基因序列的公布，可以采用定点突变的方式由野生型基因制备得到，或通过序列合成的方式获取。
实施例2
另取与实施例1中不同的IBGC患者12例以及200例正常人血样进行分析，方法同实施例2，其结果如表7所示。
表7 12例IBGC患者致病基因检测及验证结果
对于表5中IBGC患者的突变均经过Sanger测序验证了，所有突变基因均属于所述7种基因突变形式；而在200例正常人中，均不存在所述的突变，从而从正反两方面再次验证了本发明提供的IBGC致病基因，以及本发明提供的检测方法具有良好的可靠性和准确性。
虽然本发明是通过优选的具体实施例进行说明，但是对于本领域的技术人员来说，本发明可以有各种更改和变化。凡在本发明的精神和原则之内，所做的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
序列表
<110> 福建医科大学附属第一医院
<120> 第2209位点发生突变的人类IBGC致病基因PDGFRB及其检测方法
<141> 2018-05-08
<160> 35
<170> SIPOSequenceListing 1.0
<210> 1
<211> 3702
<212> DNA
<213> 智人(Homo sapiens)
<400> 1
cgcttccgga agcgggcgac tcgcagctcc acgcgacgcc gaggggctcc gcgccgggac 60
cgggcgggtg ctcggagttt cggggaccgc acgggaccga gggcaggagg agacatcaca 120
gctttcccag atcgggagga aaaatatgga atgtgtttta ccgctgactg aacacaacca 180
aatgaactgt cctgacagta gtttgcaaac cagcagctag cagtttgtcc agcctctaac 240
attgtccagc actttccaga gcaaactcac tgtttacaag aactcttggc cttacgaagt 300
ttataacctc aagctttgtt tatttaaaat attcctgcaa aagaaaagta cccggcaccc 360
actttccaaa atggccatgg atgagtattt gtggatggtc attttgggtt tcatcatagc 420
tttcatcttg gccttttctg ttggtgcaaa cgatgttgcc aactcctttg gtacagccgt 480
gggctctggt gtggtgacct tgaggcaggc atgcatttta gcttcaatat ttgaaaccac 540
cggctccgtg ttactaggcg ccaaagtagg agaaaccatt cgcaaaggta tcattgacgt 600
gaacctgtac aacgagacgg tggagactct catggctggg gaagttagtg ccatggttgg 660
ttccgctgtg tggcagctga ttgcttcctt cctgaggctt ccaatctcag gaacgcactg 720
cattgtgggt tctactatag gattctcact ggtcgcaatc ggtaccaaag gtgtgcagtg 780
gatggagctt gtcaagattg ttgcttcttg gtttatatct ccactgttgt ctggtttcat 840
gtctggcctg ctgtttgtac tcatcagaat tttcatctta aaaaaggaag accctgttcc 900
caatggcctc cgggcactcc cagtattcta tgctgctacc atagcaatca atgtcttttc 960
catcatgtac acaggagcac cagtgctcgg ccttgttctc cccatgtggg ccatagccct 1020
catttccttt ggtgtcgccc tcctgttcgc tttttttgtg tggctcttcg tgtgtccgtg 1080
gatgcggagg aaaataacag gcaaattaca aaaagaaggt gctttatcac gagtatctga 1140
cgaaagcctc agtaaggttc aggaagcaca gtccccagta tttaaagagc taccaggtgc 1200
caaggctaat gatgacagca ccatcccgct cacgggagca gcaggggaga cactggggac 1260
ctcggaaggc acttctgcgg gcagccaccc tcgggctgca tacggaagag cactgtccat 1320
gacccatggc tctgtgaaat cgcccatctc caacggcacc ttcggcttcg acggccacac 1380
caggagcgac ggtcatgtgt accacaccgt gcacaaagac tcggggctct acaaagatct 1440
gctgcacaaa atccacatcg acaggggccc cgaggagaag ccagcccagg aaagcaacta 1500
ccggctgctg cgccgaaaca acagttacac ctgctacacc gcagccattt gtgggctgcc 1560
agtgcacgcc acctttcgag ctgcggactc atcggcccca gaggacagtg agaagctggt 1620
gggcgacacc gtgtcctact ccaagaagag gctgcgctac gacagctact cgagctactg 1680
taacgcggtg gcagaggcgg agatcgaggc ggaggagggc ggcgtggaga tgaagctggc 1740
gtcggagctg gccgaccctg accagccgcg agaggaccct gcagaggagg agaaggagga 1800
gaaggacgca cccgaggttc acctcctgtt ccatttcctg caggtcctca ccgcctgttt 1860
cgggtccttt gctcacggcg gcaatgacgt gagtaatgcc atcggtcccc tggtagcctt 1920
gtggctgatt tacaaacaag gcggggtaac gcaagaagca gctacacccg tctggctgct 1980
gttttatgga ggagttggaa tctgcacagg cctctgggtc tgggggagaa gagtgatcca 2040
gaccatgggg aaggacctca ctcccatcac gccgtccagc ggcttcacga tcgagctggc 2100
ctcagccttc acagtggtga tcgcctccaa catcgggctt ccagtcagca ccacgcactg 2160
taaggtgggc tcggtggtgg ccgtgggctg gatccgctcc cgcaaggctg tggactggcg 2220
cctctttcgg aacatcttcg tggcctggtt cgtgaccgtc cctgtggctg ggctgttcag 2280
cgctgctgtc atggctcttc tcatgtatgg gatccttcca tatgtgtgag ctgtcatggc 2340
tcttctcatg tatgggatcc ttccatatgt gtgatttgtc ttcttccagc tgcaaacagc 2400
taaagggatg gtctggtgtt ggcgtgtggg agacatgtgt gctcgtgccg cacatacaca 2460
tcctggccgt gcacggctct ctcatgacca gctctctgcc tcccttccag gaggctccat 2520
cccacactgt tcacccaggc tgcggagact caccttcccg agctaactta actactgtac 2580
ataataatat gtattaaact ggtatcgtgg tgatataatg tggtgcagtt acttatatat 2640
taaatatcta ttgtatccat agaataggca gcattatttc aaacatattc aagttgggag 2700
tggagatcat tgcctagaag tcaatattca ataaatcttg tacataacta tttcgatggc 2760
aaatgttaag ccttctaaaa ggaaagtgta gattggaaaa tgattttttt tccaaatgat 2820
gtttttgcct tctaatatac tgtaaggtaa tgagcttcag aacaggcaac ctgaccctgc 2880
agaggtcgcg tgctgtggga tgacagcggg acgggagctc acaagtgctt tcactgaaga 2940
tttgttcata tactgtgtat tgattgttgt gtaatatatc atcattgctt ttgtaaatac 3000
gtaaaactgt aattttttaa tggtgtgctt cccttatact tttttgatca gagaattttg 3060
gaaagtacca aagaagcagg ggaatcattg gccagtgtta cgttttcaca ttgtctgtct 3120
cccaccctca ctgatcacgc ctgccccaga gcagtgtgtg gcggtgacac cgtcacccag 3180
catgcgccac gccgtggctc ccaccagcag tgccaccgcc accacacccc agatcccacc 3240
caccttgcag tggcctttcc ttgtcatcag agtagagaat gcacaggtgt tggtgagggc 3300
gtgtggctga gcactacatg tcaagtccag agtcagtttc tatcccaatt ctccctgcag 3360
cctgaagaac ggatccttgt ctccaatgtc agcacaaagg aggctttttc tgtgctttga 3420
cattctagca cttcagggat gagagggagg gagaatcctg gatgctggat ggagtatttc 3480
tctgaggccc acacaaagct ggacaccccc aggctctact ccatcccatt ggagtctctt 3540
ctttttttga tagcgggagg gaggaagtac gactaatgtt ggagcctgaa actatggaaa 3600
tgctgctaaa atttttatat tgacaaacat tttcttggta cttcattgtc atttttcatt 3660
aatcaaccat attaaattta taataaaaaa tgcccctcag aa 3702
<210> 2
<211> 3791
<212> DNA
<213> 智人(Homo sapiens)
<400> 2
gctcctgagg ctgccagcag ccagcagtga ctgcccgccc tatctgggac ccaggatcgc 60
tctgtgagca acttggagcc agagaggaga tcaacaagga ggaggagaga gccggcccct 120
cagccctgct gcccagcagc agcctgtgct cgccctgccc aacgcagaca gccagaccca 180
gggcggcccc tctggcggct ctgctcctcc cgaaggatgc ttggggagtg aggcgaagct 240
gggccgctcc tctcccctac agcagccccc ttcctccatc cctctgttct cctgagcctt 300
caggagcctg caccagtcct gcctgtcctt ctactcagct gttacccact ctgggaccag 360
cagtctttct gataactggg agagggcagt aaggaggact tcctggaggg ggtgactgtc 420
cagagcctgg aactgtgccc acaccagaag ccatcagcag caaggacacc atacggcttc 480
cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct ctcctgttac 540
ttctggaacc acagatctct cagggcctgg tcgtcacacc cccggggcca gagcttgtcc 600
tcaatgtctc cagcaccttc gttctgacct gctcgggttc agctccggtg gtgtgggaac 660
ggatgtccca ggagccccca caggaaatgg ccaaggccca ggatggcacc ttctccagcg 720
tgctcacact gaccaacctc actgggctag acacgggaga atacttttgc acccacaatg 780
actcccgtgg actggagacc gatgagcgga aacggctcta catctttgtg ccagatccca 840
ccgtgggctt cctccctaat gatgccgagg aactattcat ctttctcacg gaaataactg 900
agatcaccat tccatgccga gtaacagacc cacagctggt ggtgacactg cacgagaaga 960
aaggggacgt tgcactgcct gtcccctatg atcaccaacg tggcttttct ggtatctttg 1020
aggacagaag ctacatctgc aaaaccacca ttggggacag ggaggtggat tctgatgcct 1080
actatgtcta cagactccag gtgtcatcca tcaacgtctc tgtgaacgca gtgcagactg 1140
tggtccgcca gggtgagaac atcaccctca tgtgcattgt gatcgggaat gaggtggtca 1200
acttcgagtg gacatacccc cgcaaagaaa gtgggcggct ggtggagccg gtgactgact 1260
tcctcttgga tatgccttac cacatccgct ccatcctgca catccccagt gccgagttag 1320
aagactcggg gacctacacc tgcaatgtga cggagagtgt gaatgaccat caggatgaaa 1380
aggccatcaa catcaccgtg gttgagagcg gctacgtgcg gctcctggga gaggtgggca 1440
cactacaatt tgctgagctg catcggagcc ggacactgca ggtagtgttc gaggcctacc 1500
caccgcccac tgtcctgtgg ttcaaagaca accgcaccct gggcgactcc agcgctggcg 1560
aaatcgccct gtccacgcgc aacgtgtcgg agacccggta tgtgtcagag ctgacactgg 1620
ttcgcgtgaa ggtggcagag gctggccact acaccatgcg ggccttccat gaggatgctg 1680
aggtccagct ctccttccag ctacagatca atgtccctgt ccgagtgctg gagctaagtg 1740
agagccaccc tgacagtggg gaacagacag tccgctgtcg tggccggggc atgccccagc 1800
cgaacatcat ctggtctgcc tgcagagacc tcaaaaggtg tccacgtgag ctgccgccca 1860
cgctgctggg gaacagttcc gaagaggaga gccagctgga gactaacgtg acgtactggg 1920
aggaggagca ggagtttgag gtggtgagca cactgcgtct gcagcacgtg gatcggccac 1980
tgtcggtgcg ctgcacgctg cgcaacgctg tgggccagga cacgcaggag gtcatcgtgg 2040
tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc ctggtggtgc 2100
tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca cgttacgaga 2160
tccgatggaa ggtgattgag tctgtgagct ctgacggcca tgagtacatc tacgtggacc 2220
ccatgcagct gccctatgac tccacgtggg agctgccgcg ggaccagctt gtgctgggac 2280
gcaccctcgg ctctggggcc tttgggcagg tggtggaggc cacggctcat ggcctgagcc 2340
attctcaggc cacgatgaaa gtggccgtca agatgcttaa atccacagcc cgcagcagtg 2400
agaagcaagc ccttatgtcg gagctgaaga tcatgagtca ccttgggccc cacctgaacg 2460
tggtcaacct gttgggggcc tgcaccaaag gaggacccat ctatatcatc actgagtact 2520
gccgctacgg agacctggtg gactacctgc accgcaacaa acacaccttc ctgcagcacc 2580
actccgacaa gcgccgcccg cccagcgcgg agctctacag caatgctctg cccgttgggc 2640
tccccctgcc cagccatgtg tccttgaccg gggagagcga cggtggctac atggacatga 2700
gcaaggacga gtcggtggac tatgtgccca tgctggacat gaaaggagac gtcaaatatg 2760
cagacatcga gtcctccaac tacatggccc cttacgataa ctacgttccc tctgcccctg 2820
agaggacctg ccgagcaact ttgatcaacg agtctccagt gctaagctac atggacctcg 2880
tgggcttcag ctaccaggtg gccaatggca tggagtttct ggcctccaag aactgcgtcc 2940
acagagacct ggcggctagg aacgtgctca tctgtgaagg caagctggtc aagatctgtg 3000
actttggcct ggctcgagac atcatgcggg actcgaatta catctccaaa ggcagcacct 3060
ttttgccttt aaagtggatg gctccggaga gcatcttcaa cagcctctac accaccctga 3120
gcgacgtgtg gtccttcggg atcctgctct gggagatctt caccttgggt ggcacccctt 3180
acccagagct gcccatgaac gagcagttct acaatgccat caaacggggt taccgcatgg 3240
cccagcctgc ccatgcctcc gacgagatct atgagatcat gcagaagtgc tgggaagaga 3300
agtttgagat tcggcccccc ttctcccagc tggtgctgct tctcgagaga ctgttgggcg 3360
aaggttacaa aaagaagtac cagcaggtgg atgaggagtt tctgaggagt gaccacccag 3420
ccatccttcg gtcccaggcc cgcttgcctg ggttccatgg cctccgatct cccctggaca 3480
ccagctccgt cctctatact gccgtgcagc ccaatgaggg tgacaacgac tatatcatcc 3540
ccctgcctga ccccaaaccc gaggttgctg acgagggccc actggagggt tcccccagcc 3600
tagccagctc caccctgaat gaagtcaaca cctcctcaac catctcctgt gacagccccc 3660
tggagcccca ggacgaacca gagccagagc cccagcttga gctccaggtg gagccggagc 3720
cagagctgga acagttgccg gattcggggt gccctgcgcc tcgggcggaa gcagaggata 3780
gcttcctgta g 3791
<210> 3
<211> 3791
<212> DNA
<213> 智人(Homo sapiens)
<400> 3
gctcctgagg ctgccagcag ccagcagtga ctgcccgccc tatctgggac ccaggatcgc 60
tctgtgagca acttggagcc agagaggaga tcaacaagga ggaggagaga gccggcccct 120
cagccctgct gcccagcagc agcctgtgct cgccctgccc aacgcagaca gccagaccca 180
gggcggcccc tctggcggct ctgctcctcc cgaaggatgc ttggggagtg aggcgaagct 240
gggccgctcc tctcccctac agcagccccc ttcctccatc cctctgttct cctgagcctt 300
caggagcctg caccagtcct gcctgtcctt ctactcagct gttacccact ctgggaccag 360
cagtctttct gataactggg agagggcagt aaggaggact tcctggaggg ggtgactgtc 420
cagagcctgg aactgtgccc acaccagaag ccatcagcag caaggacacc atgcggcttc 480
cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct ctcctgttac 540
ttctggaacc acagatctct cagggcctgg tcgtcacacc cccggggcca gagcttgtcc 600
tcaatgtctc cagcaccttc gttctgacct gctcgggttc agctccggtg gtgtgggaac 660
ggatgtccca ggagccccca caggaaatgg ccaaggccca ggatggcacc ttctccagcg 720
tgctcacact gaccaacctc actgggctag acacgggaga atacttttgc acccacaatg 780
actcccgtgg actggagacc gatgagcgga aacggctcta catctttgtg ccagatccca 840
ccgtgggctt cctccctaat gatgccgagg aactattcat ctttctcacg gaaataactg 900
agatcaccat tccatgccga gtaacagacc cacagctggt ggtgacactg cacgagaaga 960
aaggggacgt tgcactgcct gtcccctatg atcaccaacg tggcttttct ggtatctttg 1020
aggacagaag ctacatctgc aaaaccacca ttggggacag ggaggtggat tctgatgcct 1080
actatgtcta cagactccag gtgtcatcca tcaacgtctc tgtgaacgca gtgcagactg 1140
tggtccgcca gggtgagaac atcaccctca tgtgcattgt gatcgggaat gaggtggtca 1200
acttcgagtg gacatacccc cgcaaagaaa gtgggcggct ggtggagccg gtgactgact 1260
tcctcttgga tatgccttac cacatccgct ccatcctgca catccccagt gccgagttag 1320
aagactcggg gacctacacc tgcaatgtga cggagagtgt gaatgaccat caggatgaaa 1380
aggccatcaa catcaccgtg gttgagagcg gctacgtgcg gctcctggga gaggtgggca 1440
cactacaatt tgctgagctg catcggagcc ggacactgca ggtagtgttc gaggcctacc 1500
caccgcccac tgtcctgtgg ttcaaagaca accgcaccct gggcgactcc agcgctggcg 1560
aaatcgccct gtccacgcgc aacgtgtcgg agacccggta tgtgtcagag ctgacactgg 1620
ttcgcgtgaa ggtggcagag gctggccact acaccatgcg ggccttccat gaggatgctg 1680
aggtccagct ctccttccag ctacagatca atgtccctgt ccgagtgctg gagctaagtg 1740
agagccaccc tgacagtggg gaacagacag tccgctgtcg tggccggggc atgccccagc 1800
cgaacatcat ctggtctgcc tgcagagacc tcaaaaggtg tccacgtgag ctgccgccca 1860
cgctgctggg gaacagttcc gaagaggaga gccagctgga gactaacgtg acgtactggg 1920
aggaggagca ggagtttgag gtggtgagca cactgcgtct gcagcacgtg gatcggccac 1980
tgtcggtgcg ctgcacgctg cgcaacgctg tgggccagga cacgcaggag gtcatcgtgg 2040
tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc ctggtggtgc 2100
tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca cgttacgaga 2160
tccgatggaa ggtgattgag tctgtgagct ctgacggcca tgagtacatc tacgtggacc 2220
ccatgcagct gccctatgac tccacgtggg agctgccgcg ggaccagctt gtgctgggac 2280
gcaccctcgg ctctggggcc tttgggcagg tggtggaggc cacggctcat ggcctgagcc 2340
attctcaggc cacgatgaaa gtggccgtca agatgcttaa atccacagcc cgcagcagtg 2400
agaagcaagc ccttatgtcg gagctgaaga tcatgagtca ccttgggccc cacctgaacg 2460
tggtcaacct gttgggggcc tgcaccaaag gaggacccat ctatatcatc actgagtact 2520
gccgctacgg agacctggtg gactacctgc accgcaacaa acacaccttc ctgcagcacc 2580
actccgacaa gcgccgcccg cccagcgcgg agctctacag caatgctctg cccgttgggc 2640
tccccctgcc cagccatgtg tccttgaccg gggagagcaa cggtggctac atggacatga 2700
gcaaggacga gtcggtggac tatgtgccca tgctggacat gaaaggagac gtcaaatatg 2760
cagacatcga gtcctccaac tacatggccc cttacgataa ctacgttccc tctgcccctg 2820
agaggacctg ccgagcaact ttgatcaacg agtctccagt gctaagctac atggacctcg 2880
tgggcttcag ctaccaggtg gccaatggca tggagtttct ggcctccaag aactgcgtcc 2940
acagagacct ggcggctagg aacgtgctca tctgtgaagg caagctggtc aagatctgtg 3000
actttggcct ggctcgagac atcatgcggg actcgaatta catctccaaa ggcagcacct 3060
ttttgccttt aaagtggatg gctccggaga gcatcttcaa cagcctctac accaccctga 3120
gcgacgtgtg gtccttcggg atcctgctct gggagatctt caccttgggt ggcacccctt 3180
acccagagct gcccatgaac gagcagttct acaatgccat caaacggggt taccgcatgg 3240
cccagcctgc ccatgcctcc gacgagatct atgagatcat gcagaagtgc tgggaagaga 3300
agtttgagat tcggcccccc ttctcccagc tggtgctgct tctcgagaga ctgttgggcg 3360
aaggttacaa aaagaagtac cagcaggtgg atgaggagtt tctgaggagt gaccacccag 3420
ccatccttcg gtcccaggcc cgcttgcctg ggttccatgg cctccgatct cccctggaca 3480
ccagctccgt cctctatact gccgtgcagc ccaatgaggg tgacaacgac tatatcatcc 3540
ccctgcctga ccccaaaccc gaggttgctg acgagggccc actggagggt tcccccagcc 3600
tagccagctc caccctgaat gaagtcaaca cctcctcaac catctcctgt gacagccccc 3660
tggagcccca ggacgaacca gagccagagc cccagcttga gctccaggtg gagccggagc 3720
cagagctgga acagttgccg gattcggggt gccctgcgcc tcgggcggaa gcagaggata 3780
gcttcctgta g 3791
<210> 4
<211> 1542
<212> DNA
<213> 智人(Homo sapiens)
<400> 4
gcagctcgca gctcgcagcc cgcagcccgc agaggacgcc cagagcggcg agcgggcggg 60
cagacggacc gacggactcg cgccgcgtcc acctgtcggc cgggcccagc cgagcgcgca 120
gcgggcacgc cgcgcgcgcg gagcagccgt gcccgccgcc cgggccccgc gccagggcgc 180
acacgctccc gcccccctac ccggcccggg cgggagtttg cacctctccc tgcccgggtg 240
ctcgagctgc cgttgcaaag ccaactttgg aaaaagtttt ttgggggaga cttgggcctt 300
gaggtgccca gctccgcgct ttccgatttt gggggccttt ccagaaaatg ttgcaaaaaa 360
gctaagccgg cgggcagagg aaaacgcctg tagccggcga gtgaagacga accatcgact 420
gccgtgttcc ttttcctctt ggaggttgga gtcccctggg cgcccccaca cggctagacg 480
cctcggctgg ttcgcgacgc agccccccgg ccgtggatgc tcactcgggc tcgggatccg 540
cccaggtagc ggcctcggac ccaggtcctg cgcccaggtc ctcccctgcc ccccagcgac 600
ggagccgggg ccgggggcgg cggcgcccgg gggccatgcg ggtgagccgc ggctgcagag 660
gcctgagcgc ctgatcgccg cggacccgag ccgagcccac ccccctcccc agccccccac 720
cctggccgcg ggggcggcgc gctcgatcta cgcgtccggg gccccgcggg gccgggcccg 780
gagtcggcat gaatcgctgc tgggcgctct tcctgtctct ctgctgctac ctgcgtctgg 840
tcagcgccga gggggacccc attcccgagg agctttatga gatgctgagt gaccactcga 900
tccgctcctt tgatgatctc caacgcctgc tgcacggaga ccccggagag gaagatgggg 960
ccgagttgga cctgaacatg acccgctccc actctggagg cgagctgtag agcttggctc 1020
gtggaagaag gagcctgggt tccctgacca ttgctgagcc ggccatgatc gccgagtgca 1080
agacgcgcac cgaggtgttc gagatctccc ggcgcctcat agaccgcacc aacgccaact 1140
tcctggtgtg gccgccctgt gtggaggtgc agcgctgctc cggctgctgc aacaaccgca 1200
acgtgcagtg ccgccccacc caggtgcagc tgcgacctgt ccaggtgaga aagatcgaga 1260
ttgtgcggaa gaagccaatc tttaagaagg ccacggtgac gctggaagac cacctggcat 1320
gcaagtgtga gacagtggca gctgcacggc ctgtgacccg aagcccgggg ggttcccagg 1380
agcagcgagc caaaacgccc caaactcggg tgaccattcg gacggtgcga gtccgccggc 1440
cccccaaggg caagcaccgg aaattcaagc acacgcatga caagacggca ctgaaggaga 1500
cccttggagc ctaggggcat cggcaggaga gtgtgtgggc ag 1542
<210> 5
<211> 1542
<212> DNA
<213> 智人(Homo sapiens)
<400> 5
gcagctcgca gctcgcagcc cgcagcccgc agaggacgcc cagagcggcg agcgggcggg 60
cagacggacc gacggactcg cgccgcgtcc acctgtcggc cgggcccagc cgagcgcgca 120
gcgggcacgc cgcgcgcgcg gagcagccgt gcccgccgcc cgggccccgc gccagggcgc 180
acacgctccc gcccccctac ccggcccggg cgggagtttg cacctctccc tgcccgggtg 240
ctcgagctgc cgttgcaaag ccaactttgg aaaaagtttt ttgggggaga cttgggcctt 300
gaggtgccca gctccgcgct ttccgatttt gggggccttt ccagaaaatg ttgcaaaaaa 360
gctaagccgg cgggcagagg aaaacgcctg tagccggcga gtgaagacga accatcgact 420
gccgtgttcc ttttcctctt ggaggttgga gtcccctggg cgcccccaca cggctagacg 480
cctcggctgg ttcgcgacgc agccccccgg ccgtggatgc tcactcgggc tcgggatccg 540
cccaggtagc ggcctcggac ccaggtcctg cgcccaggtc ctcccctgcc ccccagcgac 600
ggagccgggg ccgggggcgg cggcgcccgg gggccatgcg ggtgagccgc ggctgcagag 660
gcctgagcgc ctgatcgccg cggacccgag ccgagcccac ccccctcccc agccccccac 720
cctggccgcg ggggcggcgc gctcgatcta cgcgtccggg gccccgcggg gccgggcccg 780
gagtcggcat gaatcgctgc tgggcgctct tcctgtctct ctgctgctac ctgcgtctgg 840
tcagcgccga gggggacccc attcccgagg agctttatga gatgctgagt gaccactcga 900
tccgctcctt tgatgatctc caacgcctgc tgcacggaga ccccggagag gaagatgggg 960
ccgagttgga cctgaacatg acccgctccc actctggagg cgagctggag agcttggctt 1020
gtggaagaag gagcctgggt tccctgacca ttgctgagcc ggccatgatc gccgagtgca 1080
agacgcgcac cgaggtgttc gagatctccc ggcgcctcat agaccgcacc aacgccaact 1140
tcctggtgtg gccgccctgt gtggaggtgc agcgctgctc cggctgctgc aacaaccgca 1200
acgtgcagtg ccgccccacc caggtgcagc tgcgacctgt ccaggtgaga aagatcgaga 1260
ttgtgcggaa gaagccaatc tttaagaagg ccacggtgac gctggaagac cacctggcat 1320
gcaagtgtga gacagtggca gctgcacggc ctgtgacccg aagcccgggg ggttcccagg 1380
agcagcgagc caaaacgccc caaactcggg tgaccattcg gacggtgcga gtccgccggc 1440
cccccaaggg caagcaccgg aaattcaagc acacgcatga caagacggca ctgaaggaga 1500
cccttggagc ctaggggcat cggcaggaga gtgtgtgggc ag 1542
<210> 6
<211> 8614
<212> DNA
<213> 智人(Homo sapiens)
<400> 6
gcggggaggg gcggggctat ggagaggagg aggaagatgg cgggcgggct gctctgaaga 60
gacctcggcg gcggcggagg aggagagaag cgcagcgccg cgccgcgccg gggcccatgt 120
ggggaggagt cggagtcgct gttgccgccg ccgcctgtag ctgctggacc cgagtgggag 180
tgagggggaa acggcaggat gaagttcgcc gagcacctct ccgcgcacat cactcccgag 240
tggaggaagc aatacatcca gtatgaggct ttcaaggata tgctgtattc agctcaggac 300
caggcacctt ctgtggaagt tacagatgag gacacagtaa agaggtattt tgccaagttt 360
gaagagaagt ttttccaaac ctgtgaaaaa gaacttgcca aaatcaacac attttattca 420
gagaagctcg cagaggctca gcgcaggttt gctacacttc agaatgagct tcagtcatca 480
ctggatgcac agaaagaaag cactggtgtt actacgctgc gacaacgcag aaagccagtc 540
ttccacttgt cccatgagga acgtgtccaa catagaaata ttaaagacct taaactggcc 600
ttcagtgagt tctacctcag tctaatcctg ctgcagaact atcagaatct gaattttaca 660
gggtttcgaa aaatcctgaa aaagcattac aagatcctgg aaacatctcg tggagcagat 720
tggcgagtgg ctcacgtaga ggtggcccca ttttatacat gcaagaaaat caaccagctt 780
atctctgaaa ctgaggctgt agtgaccaat gaacttgaag atggtgacag acaaaaggct 840
atgaagcgtt tacgtgtccc ccctttggga gctgctcagc ctgcaccagc atggactact 900
tttagagttg gcctattttg tggaatattc attgtactga atattaccct tgtgcttgcc 960
gctgtattta aacttgaaac agatagaagt atatggccct tgataagaat ctatcggggt 1020
ggctttcttc tgattgaatt cctttttcta ctgggcatca acacgtatgg ttggagacag 1080
gctggagtaa accatgtact catctttgaa cttaatccga gaagcaattt gtctcatcaa 1140
catctctttg agattgctgg attcctcggg atattgtggt gcctgagcct tctggcatgc 1200
ttctttgctc caattagtgt catccccaca tatgtgtatc cacttgccct ttatggattt 1260
atggttttct tccttatcaa ccccaccaaa actttctact ataaatcccg gttttggctg 1320
cttaaactgc tgtttcgagt atttacagcc cccttccata aggtaggctt tgctgatttc 1380
tggctggcgg atcagctgaa cagcctgtca gtgatactga tggacctgga atatatgatc 1440
tgcttctaca gtttggagct caaatgggat gaaagtaagg gcctgttgcc aaataattca 1500
gaagaatcag gaatttgcca caaatataca tatggtgtgc gggccattgt tcagtgcatt 1560
cctgcttggc ttcgcttcat ccagtgcctg cgccgatatc gagacacaaa aagggccttt 1620
cctcatttag ttaatgctgg caaatactcc acaactttct tcatggtgac gtttgcagcc 1680
ctttacagca ctcacaaaga acgaggtcac tcggacacta tggtgttctt ttacctgtgg 1740
attgtctttt atatcatcag ttcctgctat accctcatct gggatctcaa gatggactgg 1800
ggtctcttcg ataagaatgc tggagagaac actttcctcc gggaagagat tgtatacccc 1860
caaaaagcct actactactg tgccataata gaggatgtga ttctgcgctt tgcttggact 1920
atccaaatct cgattacctc tacaactttg ttgcctcatt ctggggacat cattgctact 1980
gtctttgccc cacttgaggt tttccggcct actactactg tgccataata gaggatgtga 2040
ttctgcgctt tgcttggact atccaaatct cgattacctc tacaactttg ttgcctcatt 2100
ctggggacat cattgctact gtctttgccc cacttgaggt tttccggcga tttgtgtgga 2160
acttcttccg cctggagaat gaacatctga ataactgtgg tgaattccgt gctgtgcggg 2220
acatctctgt ggcccccctg aacgcagatg atcagactct cctagaacag atgatggacc 2280
aggatgatgg ggtacgaaac cgccagaaga atcggtcatg gaagtacaac cagagcatat 2340
ccctgcgccg gcctcgcctc gcttctcaat ccaaggctcg tgacactaag gtattgatag 2400
aagacacaga tgatgaagct aacacttgaa ttttctgaag tctagcttaa catctttggt 2460
tttcctactc tacaatcctt tcctcgacca acgcaacctc tagtaccttt ccagccgaaa 2520
acaggagaaa acacataaca cattttccga gctcttccgg atcggatcct atggactcca 2580
aacaagctca ctgtgtttct tttcttttct tctggtttaa ttttaatttt ctattttcaa 2640
aacaaatatt tacttcattt gccaatcaga ggatgtttta agaaacaaaa catagtatct 2700
tatggattgt ttacaatcac aaggacatag atacctatca ggatgaagaa caggcattgc 2760
aaggaccctc tgatgggacg gtactgagat atctcggctt ccgctcagcc cggttttgac 2820
tggttgaaac cggacattgg tttttaaatt ttttgtcagt ttatgtggag aatttttttc 2880
tttccttcat acccagcgca aaggcactgg ccgcacttgc aggaaaagtg caacttaaag 2940
cagtaccttc attcatgaag ctacttttta atttgatgta acttttctta ttttgggaag 3000
ggttgctggg tgggtgggaa atatgatgta tttgttacac atagttttct cattatttat 3060
gaaacttaac catacagaat gatataactc ctgtgcaatg aaggtgataa cagtaaaaga 3120
aggcagggga aacttacgtt ggatgacatt tatgagggtc agtcccacat acctctttca 3180
ggagacaact tgcaccagtt tgaccttttc ttttctttgt ttttatttta agccaaagtt 3240
tcattgctaa cttcttaagt tgctgctgct ttagagtcct gagcatatct ctcataacaa 3300
ggaatcccac acttcacacc accggctgaa tttcatggaa gaggttctga taattttttt 3360
aactttttaa ggaacagatg tggaatacac tggcccatat ttcaacctta acagctgaag 3420
ctatgcctta ttatgcatcc acatgtatgg tccctgtagc gtgaccttta ctagctctga 3480
atcagaagac agagctattt cagaggctct gtgtgccctc actagatagt ttttcttctg 3540
ggttcaacca ctttagccag aatttgatca aattaaaagt ctgtcatggg gaaactatat 3600
ttttgagcac atggaacaaa ttatacttcc tcattcatat tatgttgata caaaagacct 3660
tggcagccat ttctcccagc agttttaaag gatgaacatt ggatttcatg ccatcccata 3720
gaaaacctgt tttaaaattt tagggatctt tacttggtca tacatgaaaa gtacactgct 3780
tagaaattat agactattat gatctgtcca cagtgcccat tgtcacttct ttgtctcatt 3840
tcttcccttt gttccttagt catccaaata agcctgaaaa ccataagaga tattacttta 3900
ttgaatatgg ttggcattaa atttagcatt tcattatcta acaaaattaa tataaattcc 3960
aggacatggt aaaatgtgtt ttaataaccc ccagacccaa atgaaaattt caaagtcaat 4020
accagcagat tcatgaaagt aaatttagtc ctataatttt cagcttaatt ataaacaaag 4080
gaacaaataa gtggaagggc agctattacc attcgcttag tcaaaacatt cggttactgc 4140
cctttaatac actcctatca tcagcacttc caccatgtat tacaagtctt gacccatccc 4200
tgtcgtaact ccagtaaaag ttactgttac tagaaaattt ttatcaatta actgacaaat 4260
agtttctttt taaagtagtt tcttccatct ttattctgac tagcttccaa aatgtgttcc 4320
ctttttgaat cgaggttttt ttgttttgtt ttgttttctg aaaaaatcat acaactttgt 4380
gcttctattg cttttttgtg ttttgttaag catgtccctt ggcccaaatg gaagaggaaa 4440
tgtttaatta atgcttttta gtttaaataa attgaatcat ttataataat cagtgttaac 4500
aatttagtga cccttggtag gttaaaggtt gcattattta tacttgagat ttttttcccc 4560
taactattct gttttttgta ctttaaaact atgggggaaa tatcactggt ctgtcaagaa 4620
acagcagtaa ttattactga gttaaattga aaagtccagt ggaccaggca tttcttatat 4680
aaataaaatt ggtggtacta atgtgtatcc agagacattt gaattattaa ctttattctt 4740
tattagtgaa tctctatgat ggcacacata aaatcttaca gttgacttct gctgtttgaa 4800
ttccccagtg tttttacatt aatgtatcct atggccagtt tagtctttct tcaactgtta 4860
cagttctagg ccctaatcgg tcttattctt tcacatatga tgcaatgata gatgtaatcc 4920
ttttctgaag tgtttgtcgg attggcatag tctattcaaa aataaggctt atgtaacttt 4980
cagcttgcta tgtgacagaa gtttaaatgt gtatttaagt acacagcttt atgttcctct 5040
taaagctgta aagagaaaag aggacttttc ctcatcaaga gagcttggga gacaaagcat 5100
acatttaatt tttttctttc aaaaaaggca atgtgcactt tcctctccta aattttatat 5160
gccctagcag ttgcgaatgt tgtgtccacc ttacaatttg attggaggct cccatacact 5220
gaaactctca atagaattct taaaaatgaa catcagctgg gcgcagtggc tcacgtctgt 5280
aatcccagca ctttgggagg ccaaggtggg cagatcacga gctcaggagt tcgagaccag 5340
cctgaccaac atggtgaaac cccgtctcta ctaaaaatac aaaaattagc tgggcgtggt 5400
ggcgcatgcc tgtaatccca gctactcagg agactgaggc aggagaattg cttaaacctg 5460
ggaagcagag gttgcagtga gactagatca tgccactgca ctccactgcc actgcctggg 5520
caacagagca agactccatc tcaaaaacaa acaaaaaaaa tcacagactc attttaaagg 5580
agcagcaacc taaccaaata caaacttcat ttgattttag gtttagaaat tctagtttta 5640
ttttggagaa tcactcagtt tttaatatca attaaaatag tcctcaaagg aatgaagagg 5700
aagtctaaat aaatgaataa aactcatttt tcattttatg gtagtataga aagcattgat 5760
gtgtgtagag aaattaaaag acaagagtgg tttactgtct atgcacaaat gagtgcctat 5820
atttaaggct gcctatacca ttacagtggc gtaattggtg atttcatagc atacagagaa 5880
acaatgaaat caaagattaa atcattaggt tatgatggtc atttgtaaga ttggaaagta 5940
gtcaaaaacc catgtgcaac tttttttcag cactctacca ttaggctcct taggcaagct 6000
gcttaacttc cgtcagaagc aaaggtgtgg gagaacacca ttcacctcag tagtaactta 6060
taaaaaccgt ttaaagaagg aaaatgggcc ccagccccta ggatccctga cttcctgata 6120
ttctaaacat gagagttctg tgactttaac tgagctcttt accagaagta acgtgttgat 6180
gtatattatc tttccaccat gctttgacca aagtacattc acagtaacta gagaaagcag 6240
atcatccatt tctggatagg tgaactacgg caaggtaaga acagaaaaaa gtaaagtagt 6300
attgagtcat gcaggtatca gattaacaga cattcattga agataagtga aataactttg 6360
gagatagcag agaagagcaa gaagatgttg gacgttggca gtaagccgca gcaaggcagt 6420
taggaaagta cgtgagcctg atgacgcgaa ggtgatgcct ccatctgtcc gcttagtggt 6480
tattaaattg ctgggaaaga cctgccttta ttttcctatc atcttttccc cattactttg 6540
cattattcag gaaataatta tttattccca aatgcagttt tctaagcagt gaccacaaaa 6600
atttcttatt ctaaggaaaa taattaagaa aataacccta tctgtggcat tgttttaaaa 6660
tgtgggatac aaatctggta agcagatttc aggtcattat tgttttcttt taatggttta 6720
catgaaattt tcctctcaaa aaacaagtac ctcttactgt ataacatttg agtttttaaa 6780
attgagatag cttctgagag aaaaaaagaa agttgaattt gtagctctct caactacaaa 6840
atcaccacac ttttctcaaa tctaatagga attcagtgtc attacagata cggtctctag 6900
tgaccaggtg aagacaattg aagctttggt agaacttctg caaaagttag gtaaagaaga 6960
ataaatccaa tttatcagct ttgttcatat tctttttctg agtgatctaa atttttcttg 7020
cttgtgatca ttagctaaga caaggagtgt taaaggtttt ctaaatgcaa acacatacgt 7080
ttctgcattt cagaagtcag tcttgatagt aaatcctaca aactctcaac tttctgcatt 7140
gtattttaag tctatacaat gttagagcac atggtaatgt gggacagagt tgattatatg 7200
atagcatgag aaatttgcac tggttggtgt tacaaaccaa tgatctaacc agcatactct 7260
ccaaatatga aaaagtaaaa acatctcatt aggtttggaa gagcctaagg tagtccatac 7320
aggaagtttg cctgagaaca gagaggagcc tgttgattgg catggctcat gatgcctttc 7380
ttgtatgaat tatcaaaact gatactgagt tgcccctaga aaacagcctt ccagaatatg 7440
tttgcttatt tatggtggaa gaggaagtga gaacaagatg agagtttccc ttaaagtgag 7500
gagaatcatt atacaacttt gcaactgaag tatgtttcct tgtagcaaac ctttggttta 7560
ttttatcgtc aaatcaaagc caccctcagt tttttgtatg gtgccatcaa cctaaggagg 7620
acaatcaaat aacctattgt ttgccttgga ttcagtatat ctactaagta cacagtctcg 7680
tataccttca aaccagttac ttgactaaac ttccaccata ttaccttagt tcttcctatg 7740
ccctttcctc tgtgccacca caaatcagag ggaaagactg cagtacttct agaaagttgt 7800
ggacttctaa gaaagagcca ggcttccatc tcactatccc ttgatcatta tctctgaagt 7860
ccctacctgc acttccctga ttgccctgta gcaacaccag catggtggga attggaggga 7920
aagattgtgg aaaatcccta aagggtcatt gttcatgacg ttattttgaa ttgtaaaatt 7980
gtgagccact cacatttcaa ttacaaaaag aacagaactc ttaatcacgt tgtgaagatt 8040
aatttcttgc cattttctgg attagcacag aagcttgtat caatgaagag tatttaggag 8100
gggaataggg ggaaaattgt atttttctgt tcaaactctc tccttctatt tgtgtttcct 8160
tcacccctct ccctccacca cactgtgttg gtcaaatgat actattctat gaaaccctga 8220
gctatagttg tcaaaagtaa ctatggattg tgattagtcc tctgtgggtg cttccttccc 8280
tcttttttct ccctcactct ctttctcagt ctccactctg gtaaatggaa aaggtgatgt 8340
cgaagaattg atgcttgctt ggactcatgt tgtatctgtg actatgctat tagctgtctc 8400
ctttttcctt cctatatggg tagaattctt atgacttatg aagttccctt cttgataagt 8460
aggttaaatg cacaatgtgg tactgtttta tagtttctag atggttgtat aaagcaaaaa 8520
gttaatgtgg ttatgtgttt ttaaagaaaa taagtgattg gtcactaaac tctgtccttt 8580
tttcattatt acaatttctc tctgttttcc aaca 8614
<210> 7
<211> 8614
<212> DNA
<213> 智人(Homo sapiens)
<400> 7
gcggggaggg gcggggctat ggagaggagg aggaagatgg cgggcgggct gctctgaaga 60
gacctcggcg gcggcggagg aggagagaag cgcagcgccg cgccgcgccg gggcccatgt 120
ggggaggagt cggagtcgct gttgccgccg ccgcctgtag ctgctggacc cgagtgggag 180
tgagggggaa acggcaggat gaagttcgcc gagcacctct ccgcgcacat cactcccgag 240
tggaggaagc aatacatcca gtatgaggct ttcaaggata tgctgtattc agctcaggac 300
caggcacctt ctgtggaagt tacagatgag gacacagtaa agaggtattt tgccaagttt 360
gaagagaagt ttttccaaac ctgtgaaaaa gaacttgcca aaatcaacac attttattca 420
gagaagctcg cagaggctca gcgcaggttt gctacacttc agaatgagct tcagtcatca 480
ctggatgcac agaaagaaag cactggtgtt actacgctgc gacaacgcag aaagccagtc 540
ttccacttgt cccatgagga acgtgtccaa catagaaata ttaaagacct taaactggcc 600
ttcagtgagt tctacctcag tctaatcctg ctgcagaact atcagaatct gaattttaca 660
gggtttcgaa aaatcctgaa aaagcatgac aagatcctgg aaacatctcg tggagcagat 720
tggcgagtgg ctcacgtaga ggtggcccca ttttatacat gcaagaaaat caaccagctt 780
atctctgaaa ctgaggctgt agtgaccaat gaacttgaag atggtgacag acaaaaggct 840
atgaagcgtt tacgtgtccc ccctttggga gctgctcagc ctgcaccagc atggactact 900
tttagagttg gcctattttg tggaatattc attgtactga atattaccct tgtgcttgcc 960
gctgtattta aacttgaaac agatagaagt atatggccct tgataagaat ctatcggggt 1020
ggctttcttc tgattgaatt cctttttcta ctgggcatca acacgtatgg ttggagacag 1080
gctggagtaa accatgtact catctttgaa cttaatccga gaagcaattt gtctcatcaa 1140
catctctttg agattgctgg attcctcggg atattgtggt gcctgagcct tctggcatgc 1200
ttctttgctc caattagtgt catccccaca tatgtgtatc cacttgccct ttatggattt 1260
atggttttct tccttatcaa ccccaccaaa actttctact ataaatcccg gttttggctg 1320
cttaaactgc tgtttcgagt atttacagcc cccttccata aggtaggctt tgctgatttc 1380
tggctggcgg atcagctgaa cagcctgtca gtgatactga tggacctgga atatatgatc 1440
tgcttctaca gtttggagct caaatgggat gaaagtaagg gcctgttgcc aaataattca 1500
gaagaatcag gaatttgcca caaatataca tatggtgtgc gggccattgt tcagtgcatt 1560
cctgcttggc ttcgcttcat ccagtgcctg cgccgatatc gagacacaaa aagggccttt 1620
cctcatttag ttaatgctgg caaatactcc acaactttct tcatggtgac gtttgcagcc 1680
ctttacagca ctcacaaaga acgaggtcac tcggacacta tggtgttctt ttacctgtgg 1740
attgtctttt atatcatcag ttcctgctat accctcatct gggatctcaa gatggactgg 1800
ggtctcttcg ataagaatgc tggagagaac actttcctcc gggaagagat tgtatacccc 1860
caaaaagcct actactactg tgccataata gaggatgtga ttctgcgctt tgcttggact 1920
atccaaatct cgattacctc tacaactttg ttgcctcatt ctggggacat cattgctact 1980
gtctttgccc cacttgaggt tttccggcct actactactg tgccataata gaggatgtga 2040
ttctgtgctt tgcttggact atccaaatct cgattacctc tacaactttg ttgcctcatt 2100
ctggggacat cattgctact gtctttgccc cacttgaggt tttccggcga tttgtgtgga 2160
acttcttccg cctggagaat gaacatctga ataactgtgg tgaattccgt gctgtgcggg 2220
acatctctgt ggcccccctg aacgcagatg atcagactct cctagaacag atgatggacc 2280
aggatgatgg ggtacgaaac cgccagaaga atcggtcatg gaagtacaac cagagcatat 2340
ccctgcgccg gcctcgcctc gcttctcaat ccaaggctcg tgacactaag gtattgatag 2400
aagacacaga tgatgaagct aacacttgaa ttttctgaag tctagcttaa catctttggt 2460
tttcctactc tacaatcctt tcctcgacca acgcaacctc tagtaccttt ccagccgaaa 2520
acaggagaaa acacataaca cattttccga gctcttccgg atcggatcct atggactcca 2580
aacaagctca ctgtgtttct tttcttttct tctggtttaa ttttaatttt ctattttcaa 2640
aacaaatatt tacttcattt gccaatcaga ggatgtttta agaaacaaaa catagtatct 2700
tatggattgt ttacaatcac aaggacatag atacctatca ggatgaagaa caggcattgc 2760
aaggaccctc tgatgggacg gtactgagat atctcggctt ccgctcagcc cggttttgac 2820
tggttgaaac cggacattgg tttttaaatt ttttgtcagt ttatgtggag aatttttttc 2880
tttccttcat acccagcgca aaggcactgg ccgcacttgc aggaaaagtg caacttaaag 2940
cagtaccttc attcatgaag ctacttttta atttgatgta acttttctta ttttgggaag 3000
ggttgctggg tgggtgggaa atatgatgta tttgttacac atagttttct cattatttat 3060
gaaacttaac catacagaat gatataactc ctgtgcaatg aaggtgataa cagtaaaaga 3120
aggcagggga aacttacgtt ggatgacatt tatgagggtc agtcccacat acctctttca 3180
ggagacaact tgcaccagtt tgaccttttc ttttctttgt ttttatttta agccaaagtt 3240
tcattgctaa cttcttaagt tgctgctgct ttagagtcct gagcatatct ctcataacaa 3300
ggaatcccac acttcacacc accggctgaa tttcatggaa gaggttctga taattttttt 3360
aactttttaa ggaacagatg tggaatacac tggcccatat ttcaacctta acagctgaag 3420
ctatgcctta ttatgcatcc acatgtatgg tccctgtagc gtgaccttta ctagctctga 3480
atcagaagac agagctattt cagaggctct gtgtgccctc actagatagt ttttcttctg 3540
ggttcaacca ctttagccag aatttgatca aattaaaagt ctgtcatggg gaaactatat 3600
ttttgagcac atggaacaaa ttatacttcc tcattcatat tatgttgata caaaagacct 3660
tggcagccat ttctcccagc agttttaaag gatgaacatt ggatttcatg ccatcccata 3720
gaaaacctgt tttaaaattt tagggatctt tacttggtca tacatgaaaa gtacactgct 3780
tagaaattat agactattat gatctgtcca cagtgcccat tgtcacttct ttgtctcatt 3840
tcttcccttt gttccttagt catccaaata agcctgaaaa ccataagaga tattacttta 3900
ttgaatatgg ttggcattaa atttagcatt tcattatcta acaaaattaa tataaattcc 3960
aggacatggt aaaatgtgtt ttaataaccc ccagacccaa atgaaaattt caaagtcaat 4020
accagcagat tcatgaaagt aaatttagtc ctataatttt cagcttaatt ataaacaaag 4080
gaacaaataa gtggaagggc agctattacc attcgcttag tcaaaacatt cggttactgc 4140
cctttaatac actcctatca tcagcacttc caccatgtat tacaagtctt gacccatccc 4200
tgtcgtaact ccagtaaaag ttactgttac tagaaaattt ttatcaatta actgacaaat 4260
agtttctttt taaagtagtt tcttccatct ttattctgac tagcttccaa aatgtgttcc 4320
ctttttgaat cgaggttttt ttgttttgtt ttgttttctg aaaaaatcat acaactttgt 4380
gcttctattg cttttttgtg ttttgttaag catgtccctt ggcccaaatg gaagaggaaa 4440
tgtttaatta atgcttttta gtttaaataa attgaatcat ttataataat cagtgttaac 4500
aatttagtga cccttggtag gttaaaggtt gcattattta tacttgagat ttttttcccc 4560
taactattct gttttttgta ctttaaaact atgggggaaa tatcactggt ctgtcaagaa 4620
acagcagtaa ttattactga gttaaattga aaagtccagt ggaccaggca tttcttatat 4680
aaataaaatt ggtggtacta atgtgtatcc agagacattt gaattattaa ctttattctt 4740
tattagtgaa tctctatgat ggcacacata aaatcttaca gttgacttct gctgtttgaa 4800
ttccccagtg tttttacatt aatgtatcct atggccagtt tagtctttct tcaactgtta 4860
cagttctagg ccctaatcgg tcttattctt tcacatatga tgcaatgata gatgtaatcc 4920
ttttctgaag tgtttgtcgg attggcatag tctattcaaa aataaggctt atgtaacttt 4980
cagcttgcta tgtgacagaa gtttaaatgt gtatttaagt acacagcttt atgttcctct 5040
taaagctgta aagagaaaag aggacttttc ctcatcaaga gagcttggga gacaaagcat 5100
acatttaatt tttttctttc aaaaaaggca atgtgcactt tcctctccta aattttatat 5160
gccctagcag ttgcgaatgt tgtgtccacc ttacaatttg attggaggct cccatacact 5220
gaaactctca atagaattct taaaaatgaa catcagctgg gcgcagtggc tcacgtctgt 5280
aatcccagca ctttgggagg ccaaggtggg cagatcacga gctcaggagt tcgagaccag 5340
cctgaccaac atggtgaaac cccgtctcta ctaaaaatac aaaaattagc tgggcgtggt 5400
ggcgcatgcc tgtaatccca gctactcagg agactgaggc aggagaattg cttaaacctg 5460
ggaagcagag gttgcagtga gactagatca tgccactgca ctccactgcc actgcctggg 5520
caacagagca agactccatc tcaaaaacaa acaaaaaaaa tcacagactc attttaaagg 5580
agcagcaacc taaccaaata caaacttcat ttgattttag gtttagaaat tctagtttta 5640
ttttggagaa tcactcagtt tttaatatca attaaaatag tcctcaaagg aatgaagagg 5700
aagtctaaat aaatgaataa aactcatttt tcattttatg gtagtataga aagcattgat 5760
gtgtgtagag aaattaaaag acaagagtgg tttactgtct atgcacaaat gagtgcctat 5820
atttaaggct gcctatacca ttacagtggc gtaattggtg atttcatagc atacagagaa 5880
acaatgaaat caaagattaa atcattaggt tatgatggtc atttgtaaga ttggaaagta 5940
gtcaaaaacc catgtgcaac tttttttcag cactctacca ttaggctcct taggcaagct 6000
gcttaacttc cgtcagaagc aaaggtgtgg gagaacacca ttcacctcag tagtaactta 6060
taaaaaccgt ttaaagaagg aaaatgggcc ccagccccta ggatccctga cttcctgata 6120
ttctaaacat gagagttctg tgactttaac tgagctcttt accagaagta acgtgttgat 6180
gtatattatc tttccaccat gctttgacca aagtacattc acagtaacta gagaaagcag 6240
atcatccatt tctggatagg tgaactacgg caaggtaaga acagaaaaaa gtaaagtagt 6300
attgagtcat gcaggtatca gattaacaga cattcattga agataagtga aataactttg 6360
gagatagcag agaagagcaa gaagatgttg gacgttggca gtaagccgca gcaaggcagt 6420
taggaaagta cgtgagcctg atgacgcgaa ggtgatgcct ccatctgtcc gcttagtggt 6480
tattaaattg ctgggaaaga cctgccttta ttttcctatc atcttttccc cattactttg 6540
cattattcag gaaataatta tttattccca aatgcagttt tctaagcagt gaccacaaaa 6600
atttcttatt ctaaggaaaa taattaagaa aataacccta tctgtggcat tgttttaaaa 6660
tgtgggatac aaatctggta agcagatttc aggtcattat tgttttcttt taatggttta 6720
catgaaattt tcctctcaaa aaacaagtac ctcttactgt ataacatttg agtttttaaa 6780
attgagatag cttctgagag aaaaaaagaa agttgaattt gtagctctct caactacaaa 6840
atcaccacac ttttctcaaa tctaatagga attcagtgtc attacagata cggtctctag 6900
tgaccaggtg aagacaattg aagctttggt agaacttctg caaaagttag gtaaagaaga 6960
ataaatccaa tttatcagct ttgttcatat tctttttctg agtgatctaa atttttcttg 7020
cttgtgatca ttagctaaga caaggagtgt taaaggtttt ctaaatgcaa acacatacgt 7080
ttctgcattt cagaagtcag tcttgatagt aaatcctaca aactctcaac tttctgcatt 7140
gtattttaag tctatacaat gttagagcac atggtaatgt gggacagagt tgattatatg 7200
atagcatgag aaatttgcac tggttggtgt tacaaaccaa tgatctaacc agcatactct 7260
ccaaatatga aaaagtaaaa acatctcatt aggtttggaa gagcctaagg tagtccatac 7320
aggaagtttg cctgagaaca gagaggagcc tgttgattgg catggctcat gatgcctttc 7380
ttgtatgaat tatcaaaact gatactgagt tgcccctaga aaacagcctt ccagaatatg 7440
tttgcttatt tatggtggaa gaggaagtga gaacaagatg agagtttccc ttaaagtgag 7500
gagaatcatt atacaacttt gcaactgaag tatgtttcct tgtagcaaac ctttggttta 7560
ttttatcgtc aaatcaaagc caccctcagt tttttgtatg gtgccatcaa cctaaggagg 7620
acaatcaaat aacctattgt ttgccttgga ttcagtatat ctactaagta cacagtctcg 7680
tataccttca aaccagttac ttgactaaac ttccaccata ttaccttagt tcttcctatg 7740
ccctttcctc tgtgccacca caaatcagag ggaaagactg cagtacttct agaaagttgt 7800
ggacttctaa gaaagagcca ggcttccatc tcactatccc ttgatcatta tctctgaagt 7860
ccctacctgc acttccctga ttgccctgta gcaacaccag catggtggga attggaggga 7920
aagattgtgg aaaatcccta aagggtcatt gttcatgacg ttattttgaa ttgtaaaatt 7980
gtgagccact cacatttcaa ttacaaaaag aacagaactc ttaatcacgt tgtgaagatt 8040
aatttcttgc cattttctgg attagcacag aagcttgtat caatgaagag tatttaggag 8100
gggaataggg ggaaaattgt atttttctgt tcaaactctc tccttctatt tgtgtttcct 8160
tcacccctct ccctccacca cactgtgttg gtcaaatgat actattctat gaaaccctga 8220
gctatagttg tcaaaagtaa ctatggattg tgattagtcc tctgtgggtg cttccttccc 8280
tcttttttct ccctcactct ctttctcagt ctccactctg gtaaatggaa aaggtgatgt 8340
cgaagaattg atgcttgctt ggactcatgt tgtatctgtg actatgctat tagctgtctc 8400
ctttttcctt cctatatggg tagaattctt atgacttatg aagttccctt cttgataagt 8460
aggttaaatg cacaatgtgg tactgtttta tagtttctag atggttgtat aaagcaaaaa 8520
gttaatgtgg ttatgtgttt ttaaagaaaa taagtgattg gtcactaaac tctgtccttt 8580
tttcattatt acaatttctc tctgttttcc aaca 8614
<210> 8
<211> 3702
<212> DNA
<213> 智人(Homo sapiens)
<400> 8
cgcttccgga agcgggcgac tcgcagctcc acgcgacgcc gaggggctcc gcgccgggac 60
cgggcgggtg ctcggagttt cggggaccgc acgggaccga gggcaggagg agacatcaca 120
gctttcccag atcgggagga aaaatatgga atgtgtttta ccgctgactg aacacaacca 180
aatgaactgt cctgacagta gtttgcaaac cagcagctag cagtttgtcc agcctctaac 240
attgtccagc actttccaga gcaaactcac tgtttacaag aactcttggc cttacgaagt 300
ttataacctc aagctttgtt tatttaaaat attcctgcaa aagaaaagta cccggcaccc 360
actttccaaa atggccatgg atgagtattt gtggatggtc attttgggtt tcatcatagc 420
tttcatcttg gccttttctg ttggtgcaaa cgatgttgcc aactcctttg gtacagccgt 480
gggctctggt gtggtgacct tgaggcaggc atgcatttta gcttcaatat ttgaaaccac 540
cggctccgtg ttactaggcg ccaaagtagg agaaaccatt cgcaaaggta tcattgacgt 600
gaacctgtac aacgagacgg tggagactct catggctggg gaagttagtg ccatggttgg 660
ttccgctgtg tggcagctga ttgcttcctt cctgaggctt ccaatctcag gaacgcactg 720
cattgtgggt tctactatag gattctcact ggtcgcaatc ggtaccaaag gtgtgcagtg 780
gatggagctt gtcaagattg ttgcttcttg gtttatatct ccactgttgt ctggtttcat 840
gtctggcctg ctgtttgtac tcatcagaat tttcatctta aaaaaggaag accctgttcc 900
caatggcctc cgggcactcc cagtattcta tgctgctacc atagcaatca atgtcttttc 960
catcatgtac acaggagcac cagtgctcgg ccttgttctc cccatgtggg ccatagccct 1020
catttccttt ggtgtcgccc tcctgttcgc tttttttgtg tggctcttcg tgtgtccgtg 1080
gatgcggagg aaaataacag gcaaattaca aaaagaaggt gctttatcac gagtatctga 1140
cgaaagcctc agtaaggttc aggaagcaga gtccccagta tttaaagagc taccaggtgc 1200
caaggctaat gatgacagca ccatcccgct cacgggagca gcaggggaga cactggggac 1260
ctcggaaggc acttctgcgg gcagccaccc tcgggctgca tacggaagag cactgtccat 1320
gacccatggc tctgtgaaat cgcccatctc caacggcacc ttcggcttcg acggccacac 1380
caggagcgac ggtcatgtgt accacaccgt gcacaaagac tcggggctct acaaagatct 1440
gctgcacaaa atccacatcg acaggggccc cgaggagaag ccagcccagg aaagcaacta 1500
ccggctgctg cgccgaaaca acagttacac ctgctacacc gcagccattt gtgggctgcc 1560
agtgcacgcc acctttcgag ctgcggactc atcggcccca gaggacagtg agaagctggt 1620
gggcgacacc gtgtcctact ccaagaagag gctgcgctac gacagctact cgagctactg 1680
taacgcggtg gcagaggcgg agatcgaggc ggaggagggc ggcgtggaga tgaagctggc 1740
gtcggagctg gccgaccctg accagccgcg agaggaccct gcagaggagg agaaggagga 1800
gaaggacgca cccgaggttc acctcctgtt ccatttcctg caggtcctca ccgcctgttt 1860
cgggtccttt gctcacggcg gcaatgacgt gagtaatgcc atcggtcccc tggtagcctt 1920
gtggctgatt tacaaacaag gcggggtaac gcaagaagca gctacacccg tctggctgct 1980
gttttatgga ggagttggaa tctgcacagg cctctgggtc tgggggagaa gagtgatcca 2040
gaccatgggg aaggacctca ctcccatcac gccgtccagc ggcttcacga tcgagctggc 2100
ctcagccttc acagtggtga tcgcctccaa catcgggctt ccagtcagca ccacgcactg 2160
taaggtgggc tcggtggtgg ccgtgggctg gatccgctcc cgcaaggctg tggactggcg 2220
cctctttcgg aacatcttcg tggcctggtt cgtgaccgtc cctgtggctg ggctgttcag 2280
cgctgctgtc atggctcttc tcatgtatgg gatccttcca tatgtgtgag ctgtcatggc 2340
tcttctcatg tatgggatcc ttccatatgt gtgatttgtc ttcttccagc tgcaaacagc 2400
taaagggatg gtctggtgtt ggcgtgtggg agacatgtgt gctcgtgccg cacatacaca 2460
tcctggccgt gcacggctct ctcatgacca gctctctgcc tcccttccag gaggctccat 2520
cccacactgt tcacccaggc tgcggagact caccttcccg agctaactta actactgtac 2580
ataataatat gtattaaact ggtatcgtgg tgatataatg tggtgcagtt acttatatat 2640
taaatatcta ttgtatccat agaataggca gcattatttc aaacatattc aagttgggag 2700
tggagatcat tgcctagaag tcaatattca ataaatcttg tacataacta tttcgatggc 2760
aaatgttaag ccttctaaaa ggaaagtgta gattggaaaa tgattttttt tccaaatgat 2820
gtttttgcct tctaatatac tgtaaggtaa tgagcttcag aacaggcaac ctgaccctgc 2880
agaggtcgcg tgctgtggga tgacagcggg acgggagctc acaagtgctt tcactgaaga 2940
tttgttcata tactgtgtat tgattgttgt gtaatatatc atcattgctt ttgtaaatac 3000
gtaaaactgt aattttttaa tggtgtgctt cccttatact tttttgatca gagaattttg 3060
gaaagtacca aagaagcagg ggaatcattg gccagtgtta cgttttcaca ttgtctgtct 3120
cccaccctca ctgatcacgc ctgccccaga gcagtgtgtg gcggtgacac cgtcacccag 3180
catgcgccac gccgtggctc ccaccagcag tgccaccgcc accacacccc agatcccacc 3240
caccttgcag tggcctttcc ttgtcatcag agtagagaat gcacaggtgt tggtgagggc 3300
gtgtggctga gcactacatg tcaagtccag agtcagtttc tatcccaatt ctccctgcag 3360
cctgaagaac ggatccttgt ctccaatgtc agcacaaagg aggctttttc tgtgctttga 3420
cattctagca cttcagggat gagagggagg gagaatcctg gatgctggat ggagtatttc 3480
tctgaggccc acacaaagct ggacaccccc aggctctact ccatcccatt ggagtctctt 3540
ctttttttga tagcgggagg gaggaagtac gactaatgtt ggagcctgaa actatggaaa 3600
tgctgctaaa atttttatat tgacaaacat tttcttggta cttcattgtc atttttcatt 3660
aatcaaccat attaaattta taataaaaaa tgcccctcag aa 3702
<210> 9
<211> 3791
<212> DNA
<213> 智人(Homo sapiens)
<400> 9
gctcctgagg ctgccagcag ccagcagtga ctgcccgccc tatctgggac ccaggatcgc 60
tctgtgagca acttggagcc agagaggaga tcaacaagga ggaggagaga gccggcccct 120
cagccctgct gcccagcagc agcctgtgct cgccctgccc aacgcagaca gccagaccca 180
gggcggcccc tctggcggct ctgctcctcc cgaaggatgc ttggggagtg aggcgaagct 240
gggccgctcc tctcccctac agcagccccc ttcctccatc cctctgttct cctgagcctt 300
caggagcctg caccagtcct gcctgtcctt ctactcagct gttacccact ctgggaccag 360
cagtctttct gataactggg agagggcagt aaggaggact tcctggaggg ggtgactgtc 420
cagagcctgg aactgtgccc acaccagaag ccatcagcag caaggacacc atgcggcttc 480
cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct ctcctgttac 540
ttctggaacc acagatctct cagggcctgg tcgtcacacc cccggggcca gagcttgtcc 600
tcaatgtctc cagcaccttc gttctgacct gctcgggttc agctccggtg gtgtgggaac 660
ggatgtccca ggagccccca caggaaatgg ccaaggccca ggatggcacc ttctccagcg 720
tgctcacact gaccaacctc actgggctag acacgggaga atacttttgc acccacaatg 780
actcccgtgg actggagacc gatgagcgga aacggctcta catctttgtg ccagatccca 840
ccgtgggctt cctccctaat gatgccgagg aactattcat ctttctcacg gaaataactg 900
agatcaccat tccatgccga gtaacagacc cacagctggt ggtgacactg cacgagaaga 960
aaggggacgt tgcactgcct gtcccctatg atcaccaacg tggcttttct ggtatctttg 1020
aggacagaag ctacatctgc aaaaccacca ttggggacag ggaggtggat tctgatgcct 1080
actatgtcta cagactccag gtgtcatcca tcaacgtctc tgtgaacgca gtgcagactg 1140
tggtccgcca gggtgagaac atcaccctca tgtgcattgt gatcgggaat gaggtggtca 1200
acttcgagtg gacatacccc cgcaaagaaa gtgggcggct ggtggagccg gtgactgact 1260
tcctcttgga tatgccttac cacatccgct ccatcctgca catccccagt gccgagttag 1320
aagactcggg gacctacacc tgcaatgtga cggagagtgt gaatgaccat caggatgaaa 1380
aggccatcaa catcaccgtg gttgagagcg gctacgtgcg gctcctggga gaggtgggca 1440
cactacaatt tgctgagctg catcggagcc ggacactgca ggtagtgttc gaggcctacc 1500
caccgcccac tgtcctgtgg ttcaaagaca accgcaccct gggcgactcc agcgctggcg 1560
aaatcgccct gtccacgcgc aacgtgtcgg agacccggta tgtgtcagag ctgacactgg 1620
ttcgcgtgaa ggtggcagag gctggccact acaccatgcg ggccttccat gaggatgctg 1680
aggtccagct ctccttccag ctacagatca atgtccctgt ccgagtgctg gagctaagtg 1740
agagccaccc tgacagtggg gaacagacag tccgctgtcg tggccggggc atgccccagc 1800
cgaacatcat ctggtctgcc tgcagagacc tcaaaaggtg tccacgtgag ctgccgccca 1860
cgctgctggg gaacagttcc gaagaggaga gccagctgga gactaacgtg acgtactggg 1920
aggaggagca ggagtttgag gtggtgagca cactgcgtct gcagcacgtg gatcggccac 1980
tgtcggtgcg ctgcacgctg cgcaacgctg tgggccagga cacgcaggag gtcatcgtgg 2040
tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc ctggtggtgc 2100
tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca cgttacgaga 2160
tccgatggaa ggtgattgag tctgtgagct ctgacggcca tgagtacatc tacgtggacc 2220
ccatgcagct gccctatgac tccacgtggg agctgccgcg ggaccagctt gtgctgggac 2280
gcaccctcgg ctctggggcc tttgggcagg tggtggaggc cacggctcat ggcctgagcc 2340
attctcaggc cacgatgaaa gtggccgtca agatgcttaa atccacagcc cgcagcagtg 2400
agaagcaagc ccttatgtcg gagctgaaga tcatgagtca ccttgggccc cacctgaacg 2460
tggtcaacct gttgggggcc tgcaccaaag gaggacccat ctatatcatc actgagtact 2520
gccgctacgg agacctggtg gactacctgc accgcaacaa acacaccttc ctgcagcacc 2580
actccgacaa gcgccgcccg cccagcgcgg agctctacag caatgctctg cccgttgggc 2640
tccccctgcc cagccatgtg tccttgaccg gggagagcga cggtggctac atggacatga 2700
gcaaggacga gtcggtggac tatgtgccca tgctggacat gaaaggagac gtcaaatatg 2760
cagacatcga gtcctccaac tacatggccc cttacgataa ctacgttccc tctgcccctg 2820
agaggacctg ccgagcaact ttgatcaacg agtctccagt gctaagctac atggacctcg 2880
tgggcttcag ctaccaggtg gccaatggca tggagtttct ggcctccaag aactgcgtcc 2940
acagagacct ggcggctagg aacgtgctca tctgtgaagg caagctggtc aagatctgtg 3000
actttggcct ggctcgagac atcatgcggg actcgaatta catctccaaa ggcagcacct 3060
ttttgccttt aaagtggatg gctccggaga gcatcttcaa cagcctctac accaccctga 3120
gcgacgtgtg gtccttcggg atcctgctct gggagatctt caccttgggt ggcacccctt 3180
acccagagct gcccatgaac gagcagttct acaatgccat caaacggggt taccgcatgg 3240
cccagcctgc ccatgcctcc gacgagatct atgagatcat gcagaagtgc tgggaagaga 3300
agtttgagat tcggcccccc ttctcccagc tggtgctgct tctcgagaga ctgttgggcg 3360
aaggttacaa aaagaagtac cagcaggtgg atgaggagtt tctgaggagt gaccacccag 3420
ccatccttcg gtcccaggcc cgcttgcctg ggttccatgg cctccgatct cccctggaca 3480
ccagctccgt cctctatact gccgtgcagc ccaatgaggg tgacaacgac tatatcatcc 3540
ccctgcctga ccccaaaccc gaggttgctg acgagggccc actggagggt tcccccagcc 3600
tagccagctc caccctgaat gaagtcaaca cctcctcaac catctcctgt gacagccccc 3660
tggagcccca ggacgaacca gagccagagc cccagcttga gctccaggtg gagccggagc 3720
cagagctgga acagttgccg gattcggggt gccctgcgcc tcgggcggaa gcagaggata 3780
gcttcctgta g 3791
<210> 10
<211> 1542
<212> DNA
<213> 智人(Homo sapiens)
<400> 10
gcagctcgca gctcgcagcc cgcagcccgc agaggacgcc cagagcggcg agcgggcggg 60
cagacggacc gacggactcg cgccgcgtcc acctgtcggc cgggcccagc cgagcgcgca 120
gcgggcacgc cgcgcgcgcg gagcagccgt gcccgccgcc cgggccccgc gccagggcgc 180
acacgctccc gcccccctac ccggcccggg cgggagtttg cacctctccc tgcccgggtg 240
ctcgagctgc cgttgcaaag ccaactttgg aaaaagtttt ttgggggaga cttgggcctt 300
gaggtgccca gctccgcgct ttccgatttt gggggccttt ccagaaaatg ttgcaaaaaa 360
gctaagccgg cgggcagagg aaaacgcctg tagccggcga gtgaagacga accatcgact 420
gccgtgttcc ttttcctctt ggaggttgga gtcccctggg cgcccccaca cggctagacg 480
cctcggctgg ttcgcgacgc agccccccgg ccgtggatgc tcactcgggc tcgggatccg 540
cccaggtagc ggcctcggac ccaggtcctg cgcccaggtc ctcccctgcc ccccagcgac 600
ggagccgggg ccgggggcgg cggcgcccgg gggccatgcg ggtgagccgc ggctgcagag 660
gcctgagcgc ctgatcgccg cggacccgag ccgagcccac ccccctcccc agccccccac 720
cctggccgcg ggggcggcgc gctcgatcta cgcgtccggg gccccgcggg gccgggcccg 780
gagtcggcat gaatcgctgc tgggcgctct tcctgtctct ctgctgctac ctgcgtctgg 840
tcagcgccga gggggacccc attcccgagg agctttatga gatgctgagt gaccactcga 900
tccgctcctt tgatgatctc caacgcctgc tgcacggaga ccccggagag gaagatgggg 960
ccgagttgga cctgaacatg acccgctccc actctggagg cgagctggag agcttggctc 1020
gtggaagaag gagcctgggt tccctgacca ttgctgagcc ggccatgatc gccgagtgca 1080
agacgcgcac cgaggtgttc gagatctccc ggcgcctcat agaccgcacc aacgccaact 1140
tcctggtgtg gccgccctgt gtggaggtgc agcgctgctc cggctgctgc aacaaccgca 1200
acgtgcagtg ccgccccacc caggtgcagc tgcgacctgt ccaggtgaga aagatcgaga 1260
ttgtgcggaa gaagccaatc tttaagaagg ccacggtgac gctggaagac cacctggcat 1320
gcaagtgtga gacagtggca gctgcacggc ctgtgacccg aagcccgggg ggttcccagg 1380
agcagcgagc caaaacgccc caaactcggg tgaccattcg gacggtgcga gtccgccggc 1440
cccccaaggg caagcaccgg aaattcaagc acacgcatga caagacggca ctgaaggaga 1500
cccttggagc ctaggggcat cggcaggaga gtgtgtgggc ag 1542
<210> 11
<211> 8614
<212> DNA
<213> 智人(Homo sapiens)
<400> 11
gcggggaggg gcggggctat ggagaggagg aggaagatgg cgggcgggct gctctgaaga 60
gacctcggcg gcggcggagg aggagagaag cgcagcgccg cgccgcgccg gggcccatgt 120
ggggaggagt cggagtcgct gttgccgccg ccgcctgtag ctgctggacc cgagtgggag 180
tgagggggaa acggcaggat gaagttcgcc gagcacctct ccgcgcacat cactcccgag 240
tggaggaagc aatacatcca gtatgaggct ttcaaggata tgctgtattc agctcaggac 300
caggcacctt ctgtggaagt tacagatgag gacacagtaa agaggtattt tgccaagttt 360
gaagagaagt ttttccaaac ctgtgaaaaa gaacttgcca aaatcaacac attttattca 420
gagaagctcg cagaggctca gcgcaggttt gctacacttc agaatgagct tcagtcatca 480
ctggatgcac agaaagaaag cactggtgtt actacgctgc gacaacgcag aaagccagtc 540
ttccacttgt cccatgagga acgtgtccaa catagaaata ttaaagacct taaactggcc 600
ttcagtgagt tctacctcag tctaatcctg ctgcagaact atcagaatct gaattttaca 660
gggtttcgaa aaatcctgaa aaagcatgac aagatcctgg aaacatctcg tggagcagat 720
tggcgagtgg ctcacgtaga ggtggcccca ttttatacat gcaagaaaat caaccagctt 780
atctctgaaa ctgaggctgt agtgaccaat gaacttgaag atggtgacag acaaaaggct 840
atgaagcgtt tacgtgtccc ccctttggga gctgctcagc ctgcaccagc atggactact 900
tttagagttg gcctattttg tggaatattc attgtactga atattaccct tgtgcttgcc 960
gctgtattta aacttgaaac agatagaagt atatggccct tgataagaat ctatcggggt 1020
ggctttcttc tgattgaatt cctttttcta ctgggcatca acacgtatgg ttggagacag 1080
gctggagtaa accatgtact catctttgaa cttaatccga gaagcaattt gtctcatcaa 1140
catctctttg agattgctgg attcctcggg atattgtggt gcctgagcct tctggcatgc 1200
ttctttgctc caattagtgt catccccaca tatgtgtatc cacttgccct ttatggattt 1260
atggttttct tccttatcaa ccccaccaaa actttctact ataaatcccg gttttggctg 1320
cttaaactgc tgtttcgagt atttacagcc cccttccata aggtaggctt tgctgatttc 1380
tggctggcgg atcagctgaa cagcctgtca gtgatactga tggacctgga atatatgatc 1440
tgcttctaca gtttggagct caaatgggat gaaagtaagg gcctgttgcc aaataattca 1500
gaagaatcag gaatttgcca caaatataca tatggtgtgc gggccattgt tcagtgcatt 1560
cctgcttggc ttcgcttcat ccagtgcctg cgccgatatc gagacacaaa aagggccttt 1620
cctcatttag ttaatgctgg caaatactcc acaactttct tcatggtgac gtttgcagcc 1680
ctttacagca ctcacaaaga acgaggtcac tcggacacta tggtgttctt ttacctgtgg 1740
attgtctttt atatcatcag ttcctgctat accctcatct gggatctcaa gatggactgg 1800
ggtctcttcg ataagaatgc tggagagaac actttcctcc gggaagagat tgtatacccc 1860
caaaaagcct actactactg tgccataata gaggatgtga ttctgcgctt tgcttggact 1920
atccaaatct cgattacctc tacaactttg ttgcctcatt ctggggacat cattgctact 1980
gtctttgccc cacttgaggt tttccggcct actactactg tgccataata gaggatgtga 2040
ttctgcgctt tgcttggact atccaaatct cgattacctc tacaactttg ttgcctcatt 2100
ctggggacat cattgctact gtctttgccc cacttgaggt tttccggcga tttgtgtgga 2160
acttcttccg cctggagaat gaacatctga ataactgtgg tgaattccgt gctgtgcggg 2220
acatctctgt ggcccccctg aacgcagatg atcagactct cctagaacag atgatggacc 2280
aggatgatgg ggtacgaaac cgccagaaga atcggtcatg gaagtacaac cagagcatat 2340
ccctgcgccg gcctcgcctc gcttctcaat ccaaggctcg tgacactaag gtattgatag 2400
aagacacaga tgatgaagct aacacttgaa ttttctgaag tctagcttaa catctttggt 2460
tttcctactc tacaatcctt tcctcgacca acgcaacctc tagtaccttt ccagccgaaa 2520
acaggagaaa acacataaca cattttccga gctcttccgg atcggatcct atggactcca 2580
aacaagctca ctgtgtttct tttcttttct tctggtttaa ttttaatttt ctattttcaa 2640
aacaaatatt tacttcattt gccaatcaga ggatgtttta agaaacaaaa catagtatct 2700
tatggattgt ttacaatcac aaggacatag atacctatca ggatgaagaa caggcattgc 2760
aaggaccctc tgatgggacg gtactgagat atctcggctt ccgctcagcc cggttttgac 2820
tggttgaaac cggacattgg tttttaaatt ttttgtcagt ttatgtggag aatttttttc 2880
tttccttcat acccagcgca aaggcactgg ccgcacttgc aggaaaagtg caacttaaag 2940
cagtaccttc attcatgaag ctacttttta atttgatgta acttttctta ttttgggaag 3000
ggttgctggg tgggtgggaa atatgatgta tttgttacac atagttttct cattatttat 3060
gaaacttaac catacagaat gatataactc ctgtgcaatg aaggtgataa cagtaaaaga 3120
aggcagggga aacttacgtt ggatgacatt tatgagggtc agtcccacat acctctttca 3180
ggagacaact tgcaccagtt tgaccttttc ttttctttgt ttttatttta agccaaagtt 3240
tcattgctaa cttcttaagt tgctgctgct ttagagtcct gagcatatct ctcataacaa 3300
ggaatcccac acttcacacc accggctgaa tttcatggaa gaggttctga taattttttt 3360
aactttttaa ggaacagatg tggaatacac tggcccatat ttcaacctta acagctgaag 3420
ctatgcctta ttatgcatcc acatgtatgg tccctgtagc gtgaccttta ctagctctga 3480
atcagaagac agagctattt cagaggctct gtgtgccctc actagatagt ttttcttctg 3540
ggttcaacca ctttagccag aatttgatca aattaaaagt ctgtcatggg gaaactatat 3600
ttttgagcac atggaacaaa ttatacttcc tcattcatat tatgttgata caaaagacct 3660
tggcagccat ttctcccagc agttttaaag gatgaacatt ggatttcatg ccatcccata 3720
gaaaacctgt tttaaaattt tagggatctt tacttggtca tacatgaaaa gtacactgct 3780
tagaaattat agactattat gatctgtcca cagtgcccat tgtcacttct ttgtctcatt 3840
tcttcccttt gttccttagt catccaaata agcctgaaaa ccataagaga tattacttta 3900
ttgaatatgg ttggcattaa atttagcatt tcattatcta acaaaattaa tataaattcc 3960
aggacatggt aaaatgtgtt ttaataaccc ccagacccaa atgaaaattt caaagtcaat 4020
accagcagat tcatgaaagt aaatttagtc ctataatttt cagcttaatt ataaacaaag 4080
gaacaaataa gtggaagggc agctattacc attcgcttag tcaaaacatt cggttactgc 4140
cctttaatac actcctatca tcagcacttc caccatgtat tacaagtctt gacccatccc 4200
tgtcgtaact ccagtaaaag ttactgttac tagaaaattt ttatcaatta actgacaaat 4260
agtttctttt taaagtagtt tcttccatct ttattctgac tagcttccaa aatgtgttcc 4320
ctttttgaat cgaggttttt ttgttttgtt ttgttttctg aaaaaatcat acaactttgt 4380
gcttctattg cttttttgtg ttttgttaag catgtccctt ggcccaaatg gaagaggaaa 4440
tgtttaatta atgcttttta gtttaaataa attgaatcat ttataataat cagtgttaac 4500
aatttagtga cccttggtag gttaaaggtt gcattattta tacttgagat ttttttcccc 4560
taactattct gttttttgta ctttaaaact atgggggaaa tatcactggt ctgtcaagaa 4620
acagcagtaa ttattactga gttaaattga aaagtccagt ggaccaggca tttcttatat 4680
aaataaaatt ggtggtacta atgtgtatcc agagacattt gaattattaa ctttattctt 4740
tattagtgaa tctctatgat ggcacacata aaatcttaca gttgacttct gctgtttgaa 4800
ttccccagtg tttttacatt aatgtatcct atggccagtt tagtctttct tcaactgtta 4860
cagttctagg ccctaatcgg tcttattctt tcacatatga tgcaatgata gatgtaatcc 4920
ttttctgaag tgtttgtcgg attggcatag tctattcaaa aataaggctt atgtaacttt 4980
cagcttgcta tgtgacagaa gtttaaatgt gtatttaagt acacagcttt atgttcctct 5040
taaagctgta aagagaaaag aggacttttc ctcatcaaga gagcttggga gacaaagcat 5100
acatttaatt tttttctttc aaaaaaggca atgtgcactt tcctctccta aattttatat 5160
gccctagcag ttgcgaatgt tgtgtccacc ttacaatttg attggaggct cccatacact 5220
gaaactctca atagaattct taaaaatgaa catcagctgg gcgcagtggc tcacgtctgt 5280
aatcccagca ctttgggagg ccaaggtggg cagatcacga gctcaggagt tcgagaccag 5340
cctgaccaac atggtgaaac cccgtctcta ctaaaaatac aaaaattagc tgggcgtggt 5400
ggcgcatgcc tgtaatccca gctactcagg agactgaggc aggagaattg cttaaacctg 5460
ggaagcagag gttgcagtga gactagatca tgccactgca ctccactgcc actgcctggg 5520
caacagagca agactccatc tcaaaaacaa acaaaaaaaa tcacagactc attttaaagg 5580
agcagcaacc taaccaaata caaacttcat ttgattttag gtttagaaat tctagtttta 5640
ttttggagaa tcactcagtt tttaatatca attaaaatag tcctcaaagg aatgaagagg 5700
aagtctaaat aaatgaataa aactcatttt tcattttatg gtagtataga aagcattgat 5760
gtgtgtagag aaattaaaag acaagagtgg tttactgtct atgcacaaat gagtgcctat 5820
atttaaggct gcctatacca ttacagtggc gtaattggtg atttcatagc atacagagaa 5880
acaatgaaat caaagattaa atcattaggt tatgatggtc atttgtaaga ttggaaagta 5940
gtcaaaaacc catgtgcaac tttttttcag cactctacca ttaggctcct taggcaagct 6000
gcttaacttc cgtcagaagc aaaggtgtgg gagaacacca ttcacctcag tagtaactta 6060
taaaaaccgt ttaaagaagg aaaatgggcc ccagccccta ggatccctga cttcctgata 6120
ttctaaacat gagagttctg tgactttaac tgagctcttt accagaagta acgtgttgat 6180
gtatattatc tttccaccat gctttgacca aagtacattc acagtaacta gagaaagcag 6240
atcatccatt tctggatagg tgaactacgg caaggtaaga acagaaaaaa gtaaagtagt 6300
attgagtcat gcaggtatca gattaacaga cattcattga agataagtga aataactttg 6360
gagatagcag agaagagcaa gaagatgttg gacgttggca gtaagccgca gcaaggcagt 6420
taggaaagta cgtgagcctg atgacgcgaa ggtgatgcct ccatctgtcc gcttagtggt 6480
tattaaattg ctgggaaaga cctgccttta ttttcctatc atcttttccc cattactttg 6540
cattattcag gaaataatta tttattccca aatgcagttt tctaagcagt gaccacaaaa 6600
atttcttatt ctaaggaaaa taattaagaa aataacccta tctgtggcat tgttttaaaa 6660
tgtgggatac aaatctggta agcagatttc aggtcattat tgttttcttt taatggttta 6720
catgaaattt tcctctcaaa aaacaagtac ctcttactgt ataacatttg agtttttaaa 6780
attgagatag cttctgagag aaaaaaagaa agttgaattt gtagctctct caactacaaa 6840
atcaccacac ttttctcaaa tctaatagga attcagtgtc attacagata cggtctctag 6900
tgaccaggtg aagacaattg aagctttggt agaacttctg caaaagttag gtaaagaaga 6960
ataaatccaa tttatcagct ttgttcatat tctttttctg agtgatctaa atttttcttg 7020
cttgtgatca ttagctaaga caaggagtgt taaaggtttt ctaaatgcaa acacatacgt 7080
ttctgcattt cagaagtcag tcttgatagt aaatcctaca aactctcaac tttctgcatt 7140
gtattttaag tctatacaat gttagagcac atggtaatgt gggacagagt tgattatatg 7200
atagcatgag aaatttgcac tggttggtgt tacaaaccaa tgatctaacc agcatactct 7260
ccaaatatga aaaagtaaaa acatctcatt aggtttggaa gagcctaagg tagtccatac 7320
aggaagtttg cctgagaaca gagaggagcc tgttgattgg catggctcat gatgcctttc 7380
ttgtatgaat tatcaaaact gatactgagt tgcccctaga aaacagcctt ccagaatatg 7440
tttgcttatt tatggtggaa gaggaagtga gaacaagatg agagtttccc ttaaagtgag 7500
gagaatcatt atacaacttt gcaactgaag tatgtttcct tgtagcaaac ctttggttta 7560
ttttatcgtc aaatcaaagc caccctcagt tttttgtatg gtgccatcaa cctaaggagg 7620
acaatcaaat aacctattgt ttgccttgga ttcagtatat ctactaagta cacagtctcg 7680
tataccttca aaccagttac ttgactaaac ttccaccata ttaccttagt tcttcctatg 7740
ccctttcctc tgtgccacca caaatcagag ggaaagactg cagtacttct agaaagttgt 7800
ggacttctaa gaaagagcca ggcttccatc tcactatccc ttgatcatta tctctgaagt 7860
ccctacctgc acttccctga ttgccctgta gcaacaccag catggtggga attggaggga 7920
aagattgtgg aaaatcccta aagggtcatt gttcatgacg ttattttgaa ttgtaaaatt 7980
gtgagccact cacatttcaa ttacaaaaag aacagaactc ttaatcacgt tgtgaagatt 8040
aatttcttgc cattttctgg attagcacag aagcttgtat caatgaagag tatttaggag 8100
gggaataggg ggaaaattgt atttttctgt tcaaactctc tccttctatt tgtgtttcct 8160
tcacccctct ccctccacca cactgtgttg gtcaaatgat actattctat gaaaccctga 8220
gctatagttg tcaaaagtaa ctatggattg tgattagtcc tctgtgggtg cttccttccc 8280
tcttttttct ccctcactct ctttctcagt ctccactctg gtaaatggaa aaggtgatgt 8340
cgaagaattg atgcttgctt ggactcatgt tgtatctgtg actatgctat tagctgtctc 8400
ctttttcctt cctatatggg tagaattctt atgacttatg aagttccctt cttgataagt 8460
aggttaaatg cacaatgtgg tactgtttta tagtttctag atggttgtat aaagcaaaaa 8520
gttaatgtgg ttatgtgttt ttaaagaaaa taagtgattg gtcactaaac tctgtccttt 8580
tttcattatt acaatttctc tctgttttcc aaca 8614
<210> 12
<211> 25
<212> DNA
<213> 人工序列(artificial)
<400> 12
gggatccttt tgtgcattaa aacca 25
<210> 13
<211> 29
<212> DNA
<213> 人工序列(artificial)
<400> 13
gaactctctt ccagaatgtt cagtatgac 29
<210> 14
<211> 22
<212> DNA
<213> 人工序列(artificial)
<400> 14
ctggagggcc aatcaaagga tt 22
<210> 15
<211> 22
<212> DNA
<213> 人工序列(artificial)
<400> 15
ggccctgact cctgtttaac tc 22
<210> 16
<211> 23
<212> DNA
<213> 人工序列(artificial)
<400> 16
gctcccttct tctcagggtt ttt 23
<210> 17
<211> 28
<212> DNA
<213> 人工序列(artificial)
<400> 17
gtgtgtgagt gagagaagaa attcatga 28
<210> 18
<211> 22
<212> DNA
<213> 人工序列(artificial)
<400> 18
agtcttaccc aggctccttc tt 22
<210> 19
<211> 24
<212> DNA
<213> 人工序列(artificial)
<400> 19
ccaaaaatgt tggctaaaga gcca 24
<210> 20
<211> 26
<212> DNA
<213> 人工序列(artificial)
<400> 20
tgtaggacat atgctcattg gtatgc 26
<210> 21
<211> 30
<212> DNA
<213> 人工序列(artificial)
<400> 21
ctgaggagaa atttgagaaa agttggaaaa 30
<210> 22
<211> 22
<212> DNA
<213> 人工序列(artificial)
<400> 22
ccagctctgt cagtgcttag tg 22
<210> 23
<211> 30
<212> DNA
<213> 人工序列(artificial)
<400> 23
caggtaaggc atatatattc aaagctagca 30
<210> 24
<211> 21
<212> DNA
<213> 人工序列(artificial)
<400> 24
accacacctg gcctcaactt c 21
<210> 25
<211> 22
<212> DNA
<213> 人工序列(artificial)
<400> 25
tccctagctt ctgggttttc tc 22
<210> 26
<211> 19
<212> DNA
<213> 人工序列(artificial)
<400> 26
tgggacgtgg tggcttttg 19
<210> 27
<211> 19
<212> DNA
<213> 人工序列(artificial)
<400> 27
acagagccca ctggaaatc 19
<210> 28
<211> 20
<212> DNA
<213> 人工序列(artificial)
<400> 28
ctctctgggt tctaactgtc 20
<210> 29
<211> 20
<212> DNA
<213> 人工序列(artificial)
<400> 29
tctatgaaat gggcacgagg 20
<210> 30
<211> 19
<212> DNA
<213> 人工序列(artificial)
<400> 30
aggagggact gttctctgg 19
<210> 31
<211> 20
<212> DNA
<213> 人工序列(artificial)
<400> 31
ctgtgatgac tgaacacagg 20
<210> 32
<211> 20
<212> DNA
<213> 人工序列(artificial)
<400> 32
aatctttgtc cagccagctc 20
<210> 33
<211> 19
<212> DNA
<213> 人工序列(artificial)
<400> 33
gggccagcta aatactgtg 19
<210> 34
<211> 20
<212> DNA
<213> 人工序列(artificial)
<400> 34
tcgtaggaca tgtgtgagtc 20
<210> 35
<211> 19
<212> DNA
<213> 人工序列(artificial)
<400> 35
cctggccaac atggttttc 19
Claims (2)
1.第2209位点发生突变的人类IBGC致病基因PDGFB，其特征在于其核苷酸序列如SEQID NO.3所示，具体是野生型基因第2209位的G突变为A。
2.根据权利要求1所述的第2209位点发生突变的人类IBGC致病基因PDGFB的检测方法,其特征在于致病基因PDGFB检测方法的主要步骤如下:
(1)致病基因PDGFB的PCR捕获
提取待测样本基因组DNA，利用设计合成的PCR捕获引物组进行扩增；所述的PCR捕获引物组包含SEQ ID NO.12-23这12条序列；
(2)二代测序解析基因突变谱
将(1)中的PCR捕获引物组扩增产物进行二代测序，并通过信息分析找出突变位点；
(3)突变位点的验证
对于信息分析结果是如权利要求1所述的致病基因PDGFB突变形式的，则将原样本突变片段扩增后直接进行Sanger测序验证；
所述原样本突变片段扩增，采用的扩增引物对为SEQ ID NO.28-29。
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| CN110551691A (zh) * | 2018-05-31 | 2019-12-10 | 华中科技大学 | 一种特发性基底节钙化致病基因突变小鼠模型的构建 |
| CN112342206B (zh) * | 2018-05-31 | 2023-06-27 | 福建医科大学附属第一医院 | 第103位点发生突变的人类pfbc致病基因myorg及其应用 |
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| CN104232650A (zh) * | 2014-06-30 | 2014-12-24 | 深圳华大基因科技有限公司 | 特发性基底节钙化新的致病基因及其编码蛋白质和应用 |
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Also Published As
| Publication number | Publication date |
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| CN108588216A (zh) | 2018-09-28 |
| CN108841829A (zh) | 2018-11-20 |
| CN108823298A (zh) | 2018-11-16 |
| CN106834299B (zh) | 2018-06-29 |
| CN106834299A (zh) | 2017-06-13 |
| CN108531581A (zh) | 2018-09-14 |
| CN108559774B (zh) | 2021-11-26 |
| CN108588216B (zh) | 2021-09-28 |
| CN108559774A (zh) | 2018-09-21 |
| CN108531581B (zh) | 2021-09-28 |
| CN109021090B (zh) | 2021-08-31 |
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