CN109010571A - A kind of antibacterial Chinese medicine composition and preparation method thereof - Google Patents

A kind of antibacterial Chinese medicine composition and preparation method thereof Download PDF

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Publication number
CN109010571A
CN109010571A CN201811048305.4A CN201811048305A CN109010571A CN 109010571 A CN109010571 A CN 109010571A CN 201811048305 A CN201811048305 A CN 201811048305A CN 109010571 A CN109010571 A CN 109010571A
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parts
chinese medicine
extract
preparation
dandelion
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祁静
周凯
周林
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Guangzhou Huansi Instrument Technology Co Ltd
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Guangzhou Huansi Instrument Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/288—Taraxacum (dandelion)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/756—Phellodendron, e.g. corktree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00—Cosmetics or similar toilet preparations
    • A61K8/18—Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00—Cosmetics or similar toilet preparations
    • A61K8/18—Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

Abstract

The invention discloses a kind of antibacterial Chinese medicine composition, the bulk pharmaceutical chemicals of the Chinese medicine composition include dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis.Chinese medicine composition is prepared using five kinds of different Chinese medicines as raw material in the present invention, verified, the fungistatic effect of composition is better than the antibacterial effect of single medicinal material extract, embodies the synergistic effect between Chinese medicine on antibacterial effect.In the optimal situation of usage amount of each raw material, the antibacterial effect of composition is greatly promoted.

Description

A kind of antibacterial Chinese medicine composition and preparation method thereof
Technical field
The present invention relates to a kind of antibacterial Chinese medicine compositions and preparation method thereof.
Background technique
In daily life, especially spring and summer, with the increase of ambient humidity, bacterium and virus are easy to breed, and It is propagated on human skin by various communication channels, if do not handled in time it, skin disease can be caused, such as Dermatitis, eczema, psoriasis, acne, dandruff etc., when skin is damaged, human body is easy to be led by the infringement of bacterium, virus Mouth of causing injury can not heal for a long time.In terms of skin nursing, wound healing, some protective agents such as antibiotic, vitamin, microelement Preparation etc. is widely used, and there are commonly the solution such as neomycin solution, gentamicin, aureomycin, bacitracin, kasugarnycin.Change The antibiotic medicine for learning synthesis has the characteristics that effect is rapid in antibiosis, but for skin sensitivity crowd, makes for a long time With easy initiation skin dependence, while skin is reduced to the tolerance of bacterium.Traditional cflush liquid has effect mild, It is easy the advantages that being esthetically acceptable to the consumers, developing has good antibacterial, cleaning skin, the tcm product ten for promoting wound healing function Divide urgent.
Someone is attempted for the exploitation of bactericidal composition based on Chinese medicine, but current research is still in preliminary Exploratory stage, the antibacterial Chinese medicine composition that inventor has found that the prior art provides have the following problems: complicated component and type is numerous It is more, composition uncertainty risk is not only increased, the cost of Chinese Herbal Medcine Bacterial Inhibitor can be also improved；Lack compellent experiment Data；Narrow antimicrobial spectrum, antibacterial effect are bad.
One kind that CN107468972A is provided according to the understanding mechanism of Chinese traditional treatment pathogenic bacterial infection and itch has anti- The Chinese medicine composition of bacterium, anti-inflammatory and antipruritic function, each component and its mass parts ratio of the Chinese medicine composition are as follows: cnidium monnieri Son, kuh-seng, climbing groundsel, polygonum cuspidate, dandelion, dittany bark, Swamp Mahogany Leaf, the tuber of stemona, Cortex Phellodendri, the root of Dahurain angelica, pine needle, Golden Larch Bark, flos caryophylli, Alum is pointed out through clinical conditions pathogenic bacterial infection and itch effective percentage up to 95% in specification, but is provided specific Test data, especially data of antibacterial effect.
It includes Chinese mugwort extract, Herba Blumeae Balsamiferae extract that a kind of Chinese medicine composition is disclosed in CN105395397A, antibacterial Effect does not provide specific antibacterial tests data by evaluating subject sensory testing, also not yet explicitly composition to which A little bacteriums have fungistatic effect.
A kind of antibacterial Chinese medicine composition and its application are disclosed in CN108079101A, the antibacterial Chinese medicine composition is by Cortex Phellodendri Extract, Chinese straw leaf extract mix, according to its test data it is found that the composition is only capable of inhibiting Escherichia coli and gold The growth of staphylococcus aureus.
A kind of Chinese medicine composition of antibacterial anti-inflammatory is disclosed in CN104825700A, and dandelion extract is used for antibacterial and is disappeared Scorching composition, it is low in cost, drug effect is obvious；Extract of willow bark is added, is used in compounding with dandelion extract, is had significant Antibacterial anti-inflammatory advantage.But the composition is only gived in its specification to the antibacterial effect of Escherichia coli.
CN103202920A discloses a kind of Chinese medicine composition, including shrubby sophora extract and green-tea extract, but only Disclosing the composition can be used for anti-coli-infection.
Summary of the invention
The purpose of the present invention is to provide a kind of antibacterial Chinese medicine composition and its applications.
The technical solution used in the present invention is:
A kind of antibacterial Chinese medicine composition, the bulk pharmaceutical chemicals of the Chinese medicine composition include dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, dragon's blood It exhausts.
Further, in parts by weight, bulk pharmaceutical chemicals include 125~1000 portions of dandelions, 60~480 portions of Cortex Phellodendris, 20~160 Part dried orange peel, 103.8~830 parts of roots of Dahurain angelica, 12.5~100 parts of Resina Draconis.
Further, in parts by weight, bulk pharmaceutical chemicals include 125~1000 portions of dandelions, 60~480 portions of Cortex Phellodendris, 80~160 Part dried orange peel, 207.5~830 parts of roots of Dahurain angelica, 25~100 parts of Resina Draconis.
Further, in parts by weight, bulk pharmaceutical chemicals include 1000 portions of dandelions, 480 portions of Cortex Phellodendris, 160 parts of dried orange peels, 830 parts The root of Dahurain angelica, 100 parts of Resina Draconis.
Further, in parts by weight, bulk pharmaceutical chemicals include 250 portions of dandelions, 60 portions of Cortex Phellodendris, 80 parts of dried orange peels, 830 parts it is white The root of Dahurian angelica, 25 parts of Resina Draconis.
Further, in parts by weight, bulk pharmaceutical chemicals include 125 portions of dandelions, 60 portions of Cortex Phellodendris, 160 parts of dried orange peels, 207.5 parts The root of Dahurain angelica, 50 parts of Resina Draconis.
Above-mentioned antibacterial Chinese medicine composition the preparation method comprises the following steps:
Dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis are mixed, extracted using alcohol reflux；
Or dandelion extract, phellodendron extract, dried orange peel extracts, Angelica Dahurica extract, Resina Draconis are first extracted respectively and is mentioned Object is taken, then mixes extract.
Further,
Dandelion extract the preparation method comprises the following steps: dandelion is crushed after, carry out ultrasonic extraction with ethyl alcohol, filter, concentration Go solvent to get；
Phellodendron extract the preparation method comprises the following steps: Cortex Phellodendri is crushed after, with ethyl alcohol room temperature extract, filtering, concentration remove solvent, i.e., ?；
Dried orange peel extracts the preparation method comprises the following steps: take dried orange peel coarse powder, petroleum ether is added and flows back degreasing, volatilizes petroleum ether, uses Ethyl alcohol heating and refluxing extraction to get；
Angelica Dahurica extract the preparation method comprises the following steps: the root of Dahurain angelica is crushed after, with alcohol reflux, solvent is removed in filtrate filtering, concentration, i.e., ?；
Dragon's blood extract extracts the preparation method comprises the following steps: ethyl alcohol is added after Resina Draconis is crushed, and solvent is removed in filtering, concentration, To obtain the final product.
Above-mentioned antibacterial Chinese medicine composition or above-mentioned preparation method preparation antibacterial Chinese medicine composition as drug, cosmetics or The purposes of antimicrobial component in food.
A kind of cflush liquid, the antibacterial Chinese medicine combination prepared containing above-mentioned antibacterial Chinese medicine composition or above-mentioned preparation method Object.
The beneficial effects of the present invention are:
Chinese medicine composition is prepared using five kinds of different Chinese medicines as raw material in the present invention, verified, the fungistatic effect of composition Better than the antibacterial effect of single medicinal material extract, the synergistic effect between Chinese medicine on antibacterial effect is embodied.In making for each raw material In the optimal situation of dosage, the antibacterial effect of composition is greatly promoted.
Specific embodiment
The evaluation of antibacterial activity is carried out in the present invention using 5 kinds of type strains.5 kinds of type strains include: golden yellow grape Coccus (Staphylococcus aureus)；Bacillus subtilis (Bacillus subtilis)；Escherichia coli (Escherichia coli)；Candida albicans (Monilia albican)；Saccharomyces cerevisiae (Saccharomyces cerevisiae)。
In the present invention Chinese medicine composition the preparation method is as follows:
After dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis are mixed, extracted using alcohol reflux；
Or dandelion extract, phellodendron extract, dried orange peel extracts, Angelica Dahurica extract, Resina Draconis are first extracted respectively and is mentioned Object is taken, then mixes extract.
The preparation of dandelion extract: weighing dandelion and crushed, and then carries out ultrasound using appropriate 95% ethyl alcohol and mentions It takes, sets ultrasound condition are as follows: ultrasonic 25min, power 400w, interval 3-5min are repeated 3 times.Merging filtrate filters.With rotation Evaporimeter, 50 DEG C of decompression evaporation at constant temperature are concentrated to obtain the final product.
The preparation of phellodendron extract: it weighs Cortex Phellodendri and is crushed；Appropriate 55% ethyl alcohol, room temperature solution is added in smashed Cortex Phellodendri 30min is inhaled, then 60 DEG C of decompression constant temperature extract, 10min, pressure 0.04MPa, filtering；The filter residue after extracting for the first time is taken, by the The primary the same terms that extract carry out；Merge 2 filtrates, 60 DEG C of concentrated by rotary evaporations to obtain the final product.
The preparation of dried orange peel extracts: weighing dried orange peel coarse powder, is added appropriate petroleum ether (30~60 DEG C), 60 DEG C of reflux degreasings 2h；After volatilizing petroleum ether, using appropriate 60% ethyl alcohol heating and refluxing extraction to get.
The preparation of Angelica Dahurica extract: weighing the root of Dahurain angelica and crushed, then with appropriate 95% alcohol reflux, 90 DEG C of extractions 15min, 70 DEG C of extraction 2h, then 90 DEG C of extraction 30min, are filtered, by 60 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
The preparation of dragon's blood extract: weighing Resina Draconis and crushed, and 50 DEG C of appropriate 95% ethyl alcohol extractions are then added 30min, filtering, by 50 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
The bacteriostasis of the drug extract of various concentration
The preparation of phellodendron extract:
50g Cortex Phellodendri is weighed to be crushed with pulverizer；55% ethyl alcohol 250mL, room temperature desorption is added in smashed Cortex Phellodendri 30min, then 60 DEG C of decompression constant temperature extract, 10min, pressure 0.04MPa, filtering；The filter residue after extracting for the first time is taken, by first Secondary extraction the same terms carry out；Merge 2 filtrates, 60 DEG C of concentrated by rotary evaporations to 105mL.
Take sterile water that phellodendron extract is diluted to series of concentrations (drug concentration=bulk pharmaceutical chemicals quality ÷ extract volume), Its bacteriostasis is as follows:
The bacteriostasis of 1 phellodendron extract of table
Note: "-" is asepsis growth；"+" is to have bacterium growth；" * " is that can not see whether bacterium growth.
As can be seen from the results, phellodendron extract is most strong to the inhibiting effect of S. aureus L-forms, bacillus subtilis, followed by white beads Bacterium acts on saccharomyces cerevisiae unrestraint, due to, in muddy shape, visually observing after Escherichia coli and culture medium, Chinese medicine mixed culture It can not judge its bacteriostatic activity.
The preparation of Angelica Dahurica extract:
The 50g root of Dahurain angelica is weighed to be crushed；With 95% alcohol reflux of 500mL, 90 DEG C of extractions 15min, 70 DEG C of extraction 2h, so 90 DEG C of extraction 30min afterwards；Filtering, 60 DEG C of concentrated by rotary evaporations of filtrate to 60mL.
Take sterile water that Angelica Dahurica extract is diluted to series of concentrations (drug concentration=bulk pharmaceutical chemicals quality ÷ extract volume), Its bacteriostasis is as follows:
The bacteriostasis of 2 Angelica Dahurica extract of table
Note: "-" is asepsis growth；"+" is to have bacterium growth；" * " is that can not see whether bacterium growth.
As can be seen from the results, Angelica Dahurica extract has certain bacteriostatic activity to candida albicans, to bacillus subtilis, white beads Bacterium unrestraint effect, to staphylococcus aureus and Escherichia coli, then since the muddiness of culture solution can not judge.
The preparation of dragon's blood extract:
About 50g Resina Draconis is weighed, 95% ethyl alcohol 500mL is added after crushing, 50 DEG C of extraction 30min, filtrate filtering, 50 DEG C revolve Inspissation is reduced to 60mL.
Take sterile water that dragon's blood extract is diluted to series of concentrations (drug concentration=bulk pharmaceutical chemicals quality ÷ extraction object Product), bacteriostasis is as follows:
The bacteriostasis of 3 dragon's blood extract of table
"-" indicates no bacterium colony growth, and "+" indicates bacterium colony growth.
As can be seen from the results, dragon's blood extract is to staphylococcus aureus, bacillus subtilis, candida albicans and wine brewing ferment Mother has very strong bacteriostatic activity, to Escherichia coli without obvious inhibitory activity.
The preparation of dried orange peel extracts:
It weighs 50g dried orange peel coarse powder to set in 250mL flask, petroleum ether (30~60 DEG C) 800mL, 60 DEG C of reflux degreasings is added 2h；After volatilizing petroleum ether, using 60% ethyl alcohol heating and refluxing extraction, admittedly dried orange peel extracting solution about 320mL is obtained than 25 times.
Take sterile water that dried orange peel extracts are diluted to series of concentrations (drug concentration=bulk pharmaceutical chemicals quality ÷ extract volume), Its bacteriostasis is as follows:
The bacteriostasis of 4 dried orange peel extracts of table
Note: "-" is asepsis growth；"+" is to have bacterium growth；" * " is that can not see whether bacterium growth.
As can be seen from the results, dried orange peel extracts are to staphylococcus aureus, bacillus subtilis, candida albicans and saccharomyces cerevisiae There is certain bacteriostatic activity, Escherichia coli unrestraint is acted on.
The preparation of dandelion extract:
50g dandelion is weighed, is crushed with pulverizer；Ultrasonic extraction, setting ultrasound are carried out with 95% ethyl alcohol of 350mL Condition are as follows: ultrasonic 25min, power 400w, interval 3-5min are repeated 3 times；Merging filtrate filters, with Rotary Evaporators, 50 DEG C Evaporation at constant temperature concentration is depressurized, about 50mL is concentrated into.
Take sterile water that dandelion extract is diluted to series of concentrations (drug concentration=bulk pharmaceutical chemicals quality ÷ extraction object Product), bacteriostasis is as follows:
The bacteriostasis of 5 dandelion extract solution of table
Note: "-" is asepsis growth；"+" is to have bacterium growth；" * " is that can not see whether bacterium growth.
As can be seen from the results, dandelion extract inhibits most strong to staphylococcus aureus, followed by bacillus subtilis, wine Brewer yeast and candida albicans, since, in muddy shape, visually observing can not judge after Escherichia coli and culture medium, Chinese medicine mixed culture Its bacteriostatic activity.
The bacteriostasis of antibacterial Chinese medicine composition
Bacteriostasis rate %=[(control bacterium solution absorbance value-sample bacterium solution absorbance value)/control bacterium solution absorbance value] * 100%
Preparation method one:
Cortex Phellodendri, the root of Dahurain angelica, Resina Draconis, dried orange peel, dandelion extract are extracted respectively, are then mixed to get Chinese medicine antibacterial composition Finished product preparation, Chinese medicine concentration is (Chinese medicine concentration=Chinese medicine quality ÷ finished product preparation volume) as shown in the table in composition:
Antibacterial effect is as shown in the table:
It can be seen from the above result that antibacterial Chinese medicine composition is obvious to the inhibiting effect of S. aureus L-forms and bacillus subtilis, together When have certain inhibiting effect to Escherichia coli and saccharomyces cerevisiae, composition 1,12,16 simultaneously also has centainly Candida albicans Inhibiting effect.
Preparation method two:
It is mixed after a certain amount of Cortex Phellodendri, the root of Dahurain angelica, Resina Draconis, dried orange peel, dandelion are crushed, the second of 2L 95% is then added Alcohol, 60 DEG C of refluxing extraction 2h are concentrated to get Chinese medicine antibacterial composition finished product preparation, Chinese medicine concentration in composition after filtrate filtering As shown in the table (Chinese medicine concentration=Chinese medicine quality ÷ finished product preparation volume):
Its fungistatic effect is as follows:
Below in conjunction with embodiment, the present invention is further explained, it should be appreciated that following embodiment be merely to illustrate the present invention and It is not used in and limits the scope of the invention.
Embodiment 1
A kind of Chinese medicine antibacterial composition is prepared by dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis for bulk pharmaceutical chemicals.
In composition finished product preparation, Chinese medicine concentration are as follows: dandelion 1000mg/mL, Cortex Phellodendri 480mg/mL, dried orange peel 160mg/mL, root of Dahurain angelica 830mg/mL, Resina Draconis 100mg/mL.
Above-mentioned Chinese medicine concentration=Chinese medicine quality ÷ finished product preparation volume.
The preparation method comprises the following steps:
Dandelion extract, phellodendron extract, dried orange peel extracts, Angelica Dahurica extract, dragon's blood extract are first extracted respectively, Concentration, then mixes extract, and auxiliary material is added, obtains finished product preparation, each extract the preparation method is as follows:
The preparation of dandelion extract: weighing dandelion and crushed, and then carries out ultrasound using appropriate 95% ethyl alcohol and mentions It takes, sets ultrasound condition are as follows: ultrasonic 25min, power 400w, interval 3-5min are repeated 3 times.Merging filtrate filters.With rotation Evaporimeter, the concentration of 50 DEG C of decompression evaporation at constant temperature to get.
The preparation of phellodendron extract: it weighs Cortex Phellodendri and is crushed；Appropriate 55% ethyl alcohol, room temperature solution is added in smashed Cortex Phellodendri 30min is inhaled, then 60 DEG C of decompression constant temperature extract, 10min, pressure 0.04MPa, filtering；The filter residue after extracting for the first time is taken, by the The primary the same terms that extract carry out；Merge 2 filtrates, 60 DEG C of concentrated by rotary evaporations to obtain the final product.
The preparation of dried orange peel extracts: weighing dried orange peel coarse powder, is added appropriate petroleum ether (30~60 DEG C), 60 DEG C of reflux degreasings 2h；After volatilizing petroleum ether, using appropriate 60% ethyl alcohol heating and refluxing extraction to get.
The preparation of Angelica Dahurica extract: weighing the root of Dahurain angelica and crushed, then with appropriate 95% alcohol reflux, 90 DEG C of extractions 15min, 70 DEG C of extraction 2h, then 90 DEG C of extraction 30min, are filtered, by 60 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
The preparation of dragon's blood extract: weighing Resina Draconis and crushed, and 50 DEG C of appropriate 95% ethyl alcohol extractions are then added 30min, filtering, by 50 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
Embodiment 2
A kind of Chinese medicine antibacterial composition is prepared by dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis for bulk pharmaceutical chemicals.
In composition finished product preparation, Chinese medicine concentration are as follows: dandelion 250mg/mL, Cortex Phellodendri 60mg/mL, dried orange peel 80mg/ ML, root of Dahurain angelica 830mg/mL, Resina Draconis 25mg/mL.
Above-mentioned Chinese medicine concentration=Chinese medicine quality ÷ finished product preparation volume.
The preparation method comprises the following steps:
Dandelion extract, phellodendron extract, dried orange peel extracts, Angelica Dahurica extract, dragon's blood extract are first extracted respectively, Concentration, then mixes extract, and auxiliary material is added, obtains finished product preparation, each extract the preparation method is as follows:
The preparation of dandelion extract: weighing dandelion and crushed, and then carries out ultrasound using appropriate 95% ethyl alcohol and mentions It takes, sets ultrasound condition are as follows: ultrasonic 25min, power 400w, interval 3-5min are repeated 3 times.Merging filtrate filters.With rotation Evaporimeter, 50 DEG C of decompression evaporation at constant temperature concentrations, is concentrated to obtain the final product.
The preparation of phellodendron extract: it weighs Cortex Phellodendri and is crushed；Appropriate 55% ethyl alcohol, room temperature solution is added in smashed Cortex Phellodendri 30min is inhaled, then 60 DEG C of decompression constant temperature extract, 10min, pressure 0.04MPa, filtering；The filter residue after extracting for the first time is taken, by the The primary the same terms that extract carry out；Merge 2 filtrates, 60 DEG C of concentrated by rotary evaporations to obtain the final product.
The preparation of dried orange peel extracts: weighing dried orange peel coarse powder, is added appropriate petroleum ether (30~60 DEG C), 60 DEG C of reflux degreasings 2h；After volatilizing petroleum ether, using appropriate 60% ethyl alcohol heating and refluxing extraction to get.
The preparation of Angelica Dahurica extract: weighing the root of Dahurain angelica and crushed, then with appropriate 95% alcohol reflux, 90 DEG C of extractions 15min, 70 DEG C of extraction 2h, then 90 DEG C of extraction 30min, are filtered, by 60 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
The preparation of dragon's blood extract: weighing Resina Draconis and crushed, and 50 DEG C of appropriate 95% ethyl alcohol extractions are then added 30min, filtering, by 50 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
Embodiment 3
A kind of Chinese medicine antibacterial composition is prepared by dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis for bulk pharmaceutical chemicals.
In composition finished product preparation, Chinese medicine concentration are as follows: dandelion 125mg/mL, Cortex Phellodendri 60mg/mL, dried orange peel 160mg/ ML, root of Dahurain angelica 207.5mg/mL, Resina Draconis 50mg/mL.
Above-mentioned Chinese medicine concentration=Chinese medicine quality ÷ finished product preparation volume.
The preparation method comprises the following steps:
Dandelion extract, phellodendron extract, dried orange peel extracts, Angelica Dahurica extract, dragon's blood extract are first extracted respectively, Concentration, then mixes extract, and auxiliary material is added, obtains finished product preparation, each extract the preparation method is as follows:
The preparation of dandelion extract: weighing dandelion and crushed, and then carries out ultrasound using appropriate 95% ethyl alcohol and mentions It takes, sets ultrasound condition are as follows: ultrasonic 25min, power 400w, interval 3-5min are repeated 3 times.Merging filtrate filters.With rotation Evaporimeter, 50 DEG C of decompression evaporation at constant temperature concentrations, is concentrated to obtain the final product.
The preparation of phellodendron extract: it weighs Cortex Phellodendri and is crushed；Appropriate 55% ethyl alcohol, room temperature solution is added in smashed Cortex Phellodendri 30min is inhaled, then 60 DEG C of decompression constant temperature extract, 10min, pressure 0.04MPa, filtering；The filter residue after extracting for the first time is taken, by the The primary the same terms that extract carry out；Merge 2 filtrates, 60 DEG C of concentrated by rotary evaporations to obtain the final product.
The preparation of dried orange peel extracts: weighing dried orange peel coarse powder, is added appropriate petroleum ether (30~60 DEG C), 60 DEG C of reflux degreasings 2h；After volatilizing petroleum ether, using appropriate 60% ethyl alcohol heating and refluxing extraction to get.
The preparation of Angelica Dahurica extract: weighing the root of Dahurain angelica and crushed, then with appropriate 95% alcohol reflux, 90 DEG C of extractions 15min, 70 DEG C of extraction 2h, then 90 DEG C of extraction 30min, are filtered, by 60 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
The preparation of dragon's blood extract: weighing Resina Draconis and crushed, and 50 DEG C of appropriate 95% ethyl alcohol extractions are then added 30min, filtering, by 50 DEG C of concentrated by rotary evaporations of filtrate to obtain the final product.
Embodiment 4
A kind of Chinese medicine antibacterial composition is prepared by dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis for bulk pharmaceutical chemicals.
In composition finished product preparation, Chinese medicine concentration are as follows: dandelion 1000mg/mL, Cortex Phellodendri 480mg/mL, dried orange peel 160mg/mL, root of Dahurain angelica 830mg/mL, Resina Draconis 100mg/mL.
Above-mentioned Chinese medicine concentration=Chinese medicine quality ÷ finished product preparation volume.
The preparation method comprises the following steps:
It is mixed after a certain amount of Cortex Phellodendri, the root of Dahurain angelica, Resina Draconis, dried orange peel, dandelion are crushed, the second of 2L 95% is then added Alcohol, 60 DEG C of refluxing extraction 2h are concentrated to get Chinese medicine antibacterial composition finished product preparation after filtrate filtering.

Claims (10)

1. a kind of antibacterial Chinese medicine composition, it is characterised in that: the bulk pharmaceutical chemicals of the Chinese medicine composition include dandelion, Cortex Phellodendri, dried orange peel, The root of Dahurain angelica, Resina Draconis.
2. Chinese medicine composition according to claim 1, it is characterised in that: in parts by weight, bulk pharmaceutical chemicals include 125~1000 Part dandelion, 60~480 portions of Cortex Phellodendris, 20~160 parts of dried orange peels, 103.8~830 parts of roots of Dahurain angelica, 12.5~100 parts of Resina Draconis.
3. Chinese medicine composition according to claim 1, it is characterised in that: in parts by weight, bulk pharmaceutical chemicals include 125~1000 Part dandelion, 60~480 portions of Cortex Phellodendris, 80~160 parts of dried orange peels, 207.5~830 parts of roots of Dahurain angelica, 25~100 parts of Resina Draconis.
4. antibacterial Chinese medicine composition according to claim 1, it is characterised in that: in parts by weight, bulk pharmaceutical chemicals include 1000 Part dandelion, 480 portions of Cortex Phellodendris, 160 parts of dried orange peels, 830 parts of roots of Dahurain angelica, 100 parts of Resina Draconis.
5. antibacterial Chinese medicine composition according to claim 1, it is characterised in that: in parts by weight, bulk pharmaceutical chemicals include 250 parts Dandelion, 60 portions of Cortex Phellodendris, 80 parts of dried orange peels, 830 parts of roots of Dahurain angelica, 25 parts of Resina Draconis.
6. antibacterial Chinese medicine composition according to claim 1, it is characterised in that: in parts by weight, bulk pharmaceutical chemicals include 125 parts Dandelion, 60 portions of Cortex Phellodendris, 160 parts of dried orange peels, 207.5 parts of roots of Dahurain angelica, 50 parts of Resina Draconis.
7. the preparation method of the described in any item antibacterial Chinese medicine compositions of claim 1~6, it is characterised in that:
Dandelion, Cortex Phellodendri, dried orange peel, the root of Dahurain angelica, Resina Draconis are mixed, extracted using alcohol reflux；
Or dandelion extract, phellodendron extract, dried orange peel extracts, Angelica Dahurica extract, dragon's blood extract are first extracted respectively, Then extract is mixed.
8. preparation method according to claim 7, it is characterised in that:
Dandelion extract the preparation method comprises the following steps: dandelion is crushed after, carry out ultrasonic extraction with ethyl alcohol, filter, concentration go it is molten Agent to get；
Phellodendron extract the preparation method comprises the following steps: Cortex Phellodendri is crushed after, with ethyl alcohol room temperature extract, filtering, concentration go solvent to get；
Dried orange peel extracts the preparation method comprises the following steps: take dried orange peel coarse powder, petroleum ether is added and flows back degreasing, volatilizes petroleum ether, uses ethyl alcohol Heating and refluxing extraction to get；
Angelica Dahurica extract the preparation method comprises the following steps: the root of Dahurain angelica is crushed after, with alcohol reflux, filtrate filtering, concentration go solvent to get；
Dragon's blood extract the preparation method comprises the following steps: after Resina Draconis is crushed be added ethyl alcohol extract, filtering, concentration go solvent to get.
9. the described in any item antibacterial Chinese medicine compositions of claim 1~6 or the described in any item preparation sides of claim 7~8 Purposes of the antibacterial Chinese medicine composition of method preparation as antimicrobial component in drug, cosmetics or food.
10. a kind of cflush liquid, it is characterised in that: containing the described in any item antibacterial Chinese medicine compositions of claim 1~6 or The antibacterial Chinese medicine composition of the described in any item preparation method preparations of claim 7~8.
CN201811048305.4A 2018-09-10 2018-09-10 A kind of antibacterial Chinese medicine composition and preparation method thereof Pending CN109010571A (en)

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