CN109007463A - A kind of composition and application thereof for cultivating drosophila - Google Patents

Abstract

The invention discloses a kind of for cultivating the composition of drosophila, and composition contains Chinese toona fruit fruit lobe and/or Chinese toona fruit axis powder and culture drosophila basal medium；Culture drosophila basal medium contains: sucrose, agar, corn flour, propionic acid, yeast powder and water.The composition can be improved drosophila reproductive capacity, shorten drosophila preoviposition period, extend the drosophila larvae phase, shorten drosophila pupa time.

Description

A composition for cultivating fruit flies and its application

technical field

The invention belongs to the field of life sciences, and relates to a composition for cultivating fruit flies and its application.

Background technique

Chinese toon seed, also known as Chinese toon fruit, toon bud tree flower, Chinese toon bell, toon tree child, etc., is the dried fruit of Toona sinensis (A.Juss.) Roem. ], harvested in autumn and dried in the sun. According to "Tang Materia Medica", "Dictionary of Traditional Chinese Medicine", "Sichuan Traditional Chinese Medicine Chronicles" and other records, Toona sinensis is warm in nature, pungent in taste, bitter, non-toxic, and has the effects of expelling wind, expelling cold, stopping bleeding and relieving pain. All parts of Chinese toon can be used as medicine. Pharmacological studies have shown that Chinese toon has biological activities such as anti-oxidation, anti-thrombotic, anti-inflammatory, antibacterial, hypoglycemic and inhibition of cancer cell proliferation ^[2-6] . Toona sinensis is rich in a variety of pharmacologically active substances, such as flavonoids, proteins, polysaccharides, phenols, alkaloids, volatile oils and other active ingredients ^[7-11] . A large number of studies have been carried out on the isolation, extraction and identification of C.

Drosophila melanogaster is a eukaryotic multicellular organism belonging to Diptera with complete metamorphosis. Compared with other model organisms, Drosophila has the advantages of small size, easy feeding, rapid growth, short breeding cycle, and large number of offspring, as well as growth and development, metabolic system, and physiological functions that are basically similar to mammals. The most ideal model organism for the study of genetic development ^[12,13] .

There is no report on the effects of Toona sinensis or its extracts on the physiological performance of Drosophila.

references:

[1] China's state administration of traditional ahinese medicine, the chinese herbal materia medica editorial board. The chinese herbal materia medica[M]. Fifth, Shanghai: Science and Technology Press, 1999.

[2] Hseu YC, Chang WH, Chen CS, et al. Antioxidant activities of Toonasinensis leaves extracts using different antioxidant models [J]. Food and Chemical Toxicology, 2008, 46(1): 105-114.

[3] Wang CC, Tsai YJ, Hsieh YC, et al. The aqueous extract from Toonasinensis leaves inhibits microglia-mediated neuroinflammation [J]. Kaohsiung JMed Sci, 2014, 30: 73-81.

[4]HSIEHA T J, TSAIB Y H, LIAOA M C, et al.Anti-diabetic properties of non-polar Toona sinensis Roem extract prepared by supercriticalCO2 fluid[J].Food and Chemical Toxicology,2012,50(3/4):779- 789.

[5]Lin QX, Li M, Zhou RM, et al.Chemical composition and anti-bacterial activity of essential oil from Cedrela sinensis(A.Juss.) Roem.seed[J].African Journal of Biotechnology,2012,11(7) :1789-1795.

[6]Huang PJ, Hseu YC, Lee MS, et al.In vitro and in vivo activity ofgallic acid and Toona sinensis leaf extracts against HL-60 humanpremyelocytic leukemia[J].Food Chem Toxicol,2012,50(10):3489 –3497.

[7] Hou LL, Li SL, Yan YP, et al. Ding SH, Liu B, Zhao SW, et al. Anti-complement constituents from fruits of Toona sinesis [J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23 (21):91-94.

[8] Wang RS, Meng C, Zhang SJ, et al. Rapid purification of antioxidant proteins from Toona sinensis seeds using affinity chromatography [J]. Nat ProdRes Dev, 2017, 29:96-100, 86.

[9]Yan Y,Min Y,Min H,et al.n-Butanol soluble fraction of the waterextract of Chinese toon fruit ameliorated focal brain ischemic insult in ratsvia inhibition of oxidative stress and inflammation[J].J Ethnopharmaco,2014,151 :176-182.

[10]Huang XY, Li SL, Dang CZ, et al.Study on antioxidant activity and ingredient of extract from Toona Sinensis fruit[J].Seed,2011,30(7):79-82.

[11] Liu ZL, Ma TB, Sun LM, et al. Analysis of the essential oil from these seeds of Toona sinensis by GC-MS [J]. Chin Pharm J, 2002, 37(2): 94-96.

[12] Beller M, Oliver B. One hundred years of high-throughput Drosophila research [J]. Chromosome Res, 2006, 14(4): 349-362

[13] Chien S, Reiter L T, Bier E, et al. Homophila: human disease genes in Drosophila [J]. Nucleic Acids Res, 2002, 30(1): 149-151.

Contents of the invention

In order to study the pharmacological effects of Toona sinensis fruit petals and central axis, this study added different concentrations of Toona sinensis fruit petals and central axis powder to the medium of Drosophila melanogaster, and studied its effects on the duration, quantity, Sex ratio and other indicators were tested, and the effects of different parts of Toona sinensis on the development and reproduction of Drosophila melanogaster were studied and compared, in order to provide experimental basis for better development and utilization of Toona sinensis resources.

The purpose of this experiment is to study the effects of the fruit petals and axis of Toona sinensis on the developmental duration and reproductive ability of Drosophila melanogaster. The test was taken as the object of Drosophila melanogaster, and the fruit flies were cultivated with the medium (2.5g/L, 10g/L, 25g/L) that added different concentrations of Toona sinensis fruit petals and the middle axis, and the pre-oviposition period, eggs, and eggs of the fruit flies were counted. stages, larvae, pupal stages, and the ability to reproduce. The results showed that with the increase of the concentration, the effects of the toon fruit petals and the central axis on the pre-oviposition period and egg stage of the offspring Drosophila were different. Prolonged and then shortened, the impact on the egg stage of the offspring Drosophila, only the Toona sinensis fruit petals with a concentration of 2.5g/L were significantly different from the control (p﹤0.05), and the rest were not significantly different, but they all could prolong the larval stage , shorten the pupal period; both the fruit petals and the central axis of Toona sinensis can increase the number of offspring ♀ and ♂ fruit flies, and increase their proliferation rate, and the proliferation rate of the ♂ fruit fly is significantly higher than that of the ♀ fruit fly and the medium axis medium under the same concentration conditions The proliferation rates of ♀ and ♂ fruit flies were significantly higher than those of fruit petals. This shows that the fruit petals and axis of Toona sinensis can change the developmental period of Drosophila and improve the fecundity of Drosophila.

More specifically, the first aspect of the present application provides a composition, which contains Toona sinensis and a basal medium for cultivating Drosophila.

In some embodiments, the Chinese toon seed is a Chinese toon seed petal and/or a Chinese toon seed axis; and/or the fruit fly is Drosophila melanogaster.

In some embodiments, the Chinese toon seed is Chinese toon seed powder.

In some embodiments, the Chinese toon seed powder is Chinese toon seed after passing through a 40-mesh sieve.

In some embodiments, the basal medium for cultivating Drosophila contains: sucrose, agar, corn flour, propionic acid, yeast powder and water.

In some embodiments, the weight ratio of the sucrose, the agar, the corn flour, the propionic acid, the yeast powder and the water is: 50-150:5-15:60-180:5 -10:8-15:1000,

Preferably, the weight ratio of the sucrose, the agar, the corn flour, the propionic acid, the yeast powder and the water is: 31:3.1:41.3:2.5:3.5:380.

In some embodiments, the concentration of the Toona sinensis in the basal medium for cultivating Drosophila is 1-100g/L,

Preferably, the concentration of the Toona sinensis in the basal medium for cultivating Drosophila is 2.5g/L, 10g/L or 25g/L.

The second aspect of the present application provides the use of the composition described in the first aspect of the present application in improving the fecundity of fruit flies.

The third aspect of the present application provides the use of the composition as described in the first aspect of the present application in shortening the pre-oviposition period and/or the pupal period of Drosophila.

The fourth aspect of the present application provides the use of the composition described in the first aspect of the present application in prolonging the larval stage of Drosophila.

The composition can improve the fecundity of fruit flies, shorten the pre-oviposition period of fruit flies, prolong the larval period of fruit flies and shorten the pupal period of fruit flies.

Detailed ways

In order to better explain the technical solution of the present invention, the following describes the embodiments of the present invention in detail. The following examples are used to further illustrate the present invention, but should not be construed as fixing or limiting the present invention. Unless otherwise specified, the technical features used in the embodiments may be replaced with other technical features known in the art that have equivalent or similar functions or effects without departing from the inventive concept.

1 Materials and methods

1.1 Materials and reagents

Toona sinensis, purchased from Anhui Bozhou Medicinal Materials Market, produced in Taihe, Anhui, dried naturally for later use;

Wild-type Drosophila melanogaster, provided by the Genetics Laboratory of the School of Biology and Food Engineering, Fuyang Normal University, was raised on a cornmeal-sucrose medium with a culture temperature of 25±1°C, a humidity of 60%-70%, and a photoperiod of Light and dark were 12 hours each; propionic acid, sucrose, yeast powder, and agar were all analytically pure (Sinopharm Chemical Reagent Co., Ltd.), and corn flour was purchased from Fuyang Hualian Supermarket.

1.2 Instruments and equipment

Instrument: GZL-P800B intelligent light incubator: Hefei Dascart Scientific Equipment Company; GI54TW automatic vertical autoclave: Zhiwei (Xiamen) Instrument Co., Ltd.; FST-GX-80 Prefill Ultrapure water machine: Shanghai Fushite Instrument Co., Ltd.; FSH-Z adjustable high-speed homogenizer: Jintan Jerry Electric Co., Ltd.; FA2204 electronic balance: Tianjin Tianma Hengji Instrument Co., Ltd.

1.3 Experimental method

1.3.1 Prepare medium

Separate the dried petals and the central axis of the Chinese toon seeds, crush them appropriately, pass through a 40-mesh sieve, and store them for future use. Three kinds of culture media were used in the experiment: basal culture medium (CK), Chinese toon seed petal culture medium (Ⅰ) and Chinese toon seed medium axis medium (Ⅱ).

Preparation of basal medium (CK): Weigh 31g of sucrose, 3.1g of agar, 41.3g of corn flour, add 380mL of water, and boil for 3 times to completely dissolve the agar powder. After cooling down slightly, add 2.5mL propionic acid and stir thoroughly, and finally add 3.5g yeast powder, stir evenly and distribute it into culture tubes as a blank control group; The basal medium was added with fruit petals and central axis powders respectively, so that the final concentrations were 2.5g/L, 10g/L, and 25g/L, respectively.

1.3.2 Collection of virgin flies

It is generally believed that female flies that have not mated within 8 hours of eclosion are called virgin flies. Insert male and female Drosophila melanogaster into the freshly prepared basal medium, put them in the incubator and cultivate them for 7 days, remove the adult flies, and continue culturing in the culture tube until some adults emerge. After ether anesthesia, the male and female flies were separated with a magnifying glass, and the healthy and vigorous fruit flies were selected for the experiment.

1.3.3 Observe and record the development and reproduction of the F1 generation of Drosophila melanogaster

The ♀ and ♂ Drosophila collected according to the method in 1.3.2 were randomly divided into groups, and bred and bred in mediums Ⅰ and Ⅱ containing different concentrations respectively, and CK was used as a blank control group. Adults of ♀ and ♂ are paired at a ratio of 1:1, 1 pair/tube, and 12 replicates are set up for each concentration of different media. There are F1 generation adults formed. During this period, observe and record the pre-oviposition stage, egg hatching, larval pupation, and pupae emergence of each group of fruit flies every 8 hours, and calculate the time required for the development of each growth stage of the offspring fruit flies. The number of male and female offspring (F1) and the total number of adult flies were counted for 10 days in a row, and the ratio of male and female offspring and the proliferation rate were calculated. Among them, the pre-oviposition period is the time from the mating of male and female fruit flies to the appearance of eggs in the culture tube; the egg stage is the time from egg development to the emergence of first-instar larvae; the larval period is the time from the development of first-instar larvae to emergence of pupae The pupal stage is the time elapsed from pupation to adult eclosion.

1.3.4 Observe and record the development and reproduction of the F2 generation of Drosophila melanogaster

The F1 individuals within 8 hours of eclosion were collected, divided into random groups, and bred in medium containing different concentrations of Ⅰ and Ⅱ, respectively, and CK was used as a blank control group. Observe and record the pre-oviposition stage, egg hatching, larval pupation and pupae emergence of each group of Drosophila every 8 hours, and calculate the time required for each growth stage of the F2 generation Drosophila.

1.3.4 Statistical analysis of data

SPSS21.0 statistical analysis software was used for statistical analysis of the data, and the test data was expressed as "mean ± standard deviation".

2 Results and Analysis

2.1 Effects of different components of Toona sinensis on the developmental duration of different stages of F1 generation fruit flies

See Table 1 for the developmental periods of different stages of the F1 generation Drosophila in different concentrations of Toona sinensis fruit petals and central axis treatment groups. Compared with the control group, the pre-oviposition period of the F1 generation Drosophila under the condition of Chinese toon fruit flap medium shortened as the concentration increased, and when the concentration was 10g/L and 25g/L, there was a significant difference from the control (p﹤0.05 ) or extremely significant (p﹤0.01), at this time the pre-oviposition period was 31.36h and 24h respectively; this medium had little effect on the egg stage, only when the concentration was 2.5g/L and the control reached a significant difference (p﹤ 0.05), prolonging 2.8h; when the concentration of Toona sinensis fruit petals was 25g/L, the larval stage of F1 generation fruit flies was extended by 14.4h (p﹤0.05), and the pupal stage was shortened by 15.6h (p﹤0.01).

In the same mode as the Chinese toon seed petal medium, the effect of the Chinese toon seed axis medium on the egg stage of the F1 generation Drosophila was not significantly different from that of the control, while the effect on the early stage of oviposition was first with the increase of the concentration. Shorten after prolongation (4.08h, 7.44h, shortened 19.92h respectively), prolong the larval stage of Drosophila by a maximum of 67.2h, shorten the pupal stage by a maximum of 24h, and both at concentrations of 10g/L and 25g/L The difference between L and the control was extremely significant (p﹤0.01).

By comparing the influence of the different Toona sinensis parts medium at the same concentration on the developmental period of Drosophila, it was found that the same concentration of Toona sinensis fruit petals and the central axis medium had no significant difference in the pupal stage of the F1 generation Drosophila. The effects on the larval stage were all significantly different (p﹤0.01), and the maximum difference was 52.8h; when the concentration was 2.5g/L and 10g/L, the difference was extremely significant (p﹤0.01). 0.01), the time gaps required at this time are 8.54h and 20h respectively, see Table 1. According to the analysis, the reason for this phenomenon may be that the effective active ingredients and pharmacological effects contained in the fruit petal and the central axis are different.

Table 1 Effects of the fruit petals and central axes of Toona sinensis on the developmental duration of different stages of F1 generation Drosophila

Tab.1 Effects of carpel and axial of fructus T.Sinensis at different dosages on the developmental duration of F1 of drosophilas

Note: different lowercase letters after the data in the same column and the same group indicate significant differences (P<0.05); different uppercase letters after the data in the same column and the same group indicate extremely significant differences (P<0.01); different lowercase letters after the data in the same column and the same concentration Letters indicate significant differences (P<0.05); different uppercase letters in the subscript after the data in the same column and the same concentration indicate extremely significant differences (P<0.01). The same below.

2.2 Effects of different components of Toona sinensis on the developmental duration of different stages of F2 generation fruit flies

Compared with the control group, it was found that the effects of different concentrations of Toona sinensis fruit valve medium on the pre-oviposition, egg, larval and pupal stages of F2 generation Drosophila were not significantly different.

And different concentrations of Chinese Toona sinensis media have no significant impact on the egg stage of the F2 generation fruit flies and the contrast difference, other concentrations are all along with the increase of the concentration, so that the early stage of oviposition of the fruit flies is first extended and then shortened (respectively 7.52h, 12.75h, shortened 25.8h), the larval stage was prolonged (respectively extended 21.51h, 28.21h, 68.5h), the pupal stage was shortened (respectively shortened 15.83h, 34.34h, 48.03h), compared with the control, all The difference was significant (p﹤0.05) or extremely significant (p﹤0.01), see Table 2.

It can also be seen from Table 2 that, except for the egg stage, the same concentration of Toona sinensis fruit petals and the central axis medium have significant differences in the pre-oviposition, larval, and pupal stages of F2 generation fruit flies (p﹤0.05) Or extremely significant (p﹤0.01), and the difference was the largest under the condition of 25g/L concentration, which were 20.8h, 73.44h and 44.09h respectively. Through comparison, it is also found that the media of the Chinese toon seed axis has more obvious effects on the pre-oviposition stage, larval stage, and pupal stage of Drosophila fruit flies than the fruit petals, and it can continue to the offspring, and the degree is continuously deepened.

Table 2 Effects of the fruit petals and central axis of Toona sinensis on the developmental duration of different stages of F2 generation Drosophila

Tab.2 Effects of carpel and axial of fructusT.Sinensis at different dosages on the developmental duration of F2 of drosophilas

2.3 Effects of different components of Toona sinensis on the fecundity of Drosophila F1 generation

After fruit flies were cultivated in different concentrations of Toona sinensis fruit petals and central axis medium, the number of F1 generation ♀ and ♂ fruit flies compared with the control, and the number all increased (except the 25g/L concentration fruit petal medium) (Table 3), It shows that the fruit petals and central axis of Toona sinensis have the ability to promote the reproduction of Drosophila. The number of F1 generation ♀ Drosophila increased first and then decreased, and only when the concentration was 25g/L, the number was lower than that of the control group and was significantly different from the control group (p﹤0.05). is -5.95%. The proliferation rate of F1 generation ♂ fruit flies showed a trend of first increasing and then decreasing with the increase of the concentration of Toona sinensis fruit petal medium. When the concentration was 10g/L, the number of ♂ fruit flies was significantly different from the control (p﹤0.05) , the proliferation rate was the highest at this time, which was 41.69%. The influence of the Chinese toon seed axis medium on the number of F1 generation ♀ and ♂ fruit flies was that it first increased and then gradually decreased with the increase of the concentration, but they were all higher than the control group ♀, ♂ For the number of ♂ fruit flies, when the concentration is 2.5g/L, the proliferation rate of ♀ and ♂ fruit flies reaches the maximum, which are 61.71% and 80.18% respectively.

Further comparison found that the number of F1 generation ♀ and ♂ fruit flies under the condition of the same concentration of Toona sinensis fruit petals and the central axis medium, except that the number of ♂ fruit flies under the condition of 10g/L concentration was not significantly different, the rest were all different. Significant (p﹤0.05) or extremely significant (p﹤0.01), see Table 3. Through the comparison of the proliferation rate of the F1 generation ♀ and ♂ fruit flies, it was found that the effect of the fruit petals and the central axis of Toona sinensis on the number of ♂ fruit flies was more obvious than that of ♀ fruit flies, but both of them had little effect on the sex ratio of ♀ and ♂ fruit flies ( table 3).

Table 3 The fecundity of Drosophila F1 generation under the conditions of different concentrations of Toona sinensis fruit petals and central axis medium

Tab.3 The effect of different concentrations of carpel and axial ofructus T.Sinensis on the F1 generation of fertility

2.4 Effects of different components of Toona sinensis on the fecundity of Drosophila F2 generation

As shown in Table 4, different concentrations of Toona sinensis fruit petals and central axis also increased the number of F2 generation ♀ and ♂ fruit flies. By comparison, it was found that with the increase of the concentration of the Chinese toon fruit petal medium, the number of F2 generation ♀ and ♂ fruit flies first increased and then decreased, but they were all higher than the control group, and only when the concentration was 10g/L, the number of F2 The difference between the number of ♂ fruit flies and the control was extremely significant (p﹤0.01), and the proliferation rate was 29.48% at this time; while the number of F2 generation ♀ and ♂ fruit flies under the condition of different concentrations of Chinese toon seed axis medium was compared with the control, All reached extremely significant differences (p﹤0.01), and when the concentration was 2.5g/L, the number of ♀ and ♂ fruit flies was the largest, and the proliferation rates were 77.91% and 87.39%, respectively, which were higher than the F1 generation under the same concentration conditions. The multiplication rate of ♀ and ♂ indicated that the axillary axis of Toona sinensis had a more significant ability to enhance the reproduction of fruit flies, and there was a cumulative effect from generation to generation.

It can also be seen from Table 4 that the number of F2 generation ♀ and ♂ fruit flies under the conditions of the Chinese toon seed petals and the central axis culture medium showed extremely significant differences (p﹤0.01) under the same concentration conditions (p﹤0.01), that is, the central axis medium The number of F2 ♀ and ♂ fruit flies in the medium was significantly higher than that in the fruit petal medium, which indicated that the ability of the central axis of Chinese toon seed to promote the reproduction of fruit flies was better than that of the fruit petal of Chinese toon seed. Like the F1 generation, the axis and fruit petals of Toona sinensis had little effect on the sex ratio of the F2 generation ♀ and ♂ Drosophila.

Table 4 The fecundity of Drosophila F2 generation under the conditions of different concentrations of Toona sinensis fruit petals and central axis medium

Tab.4 The effect of different concentrations of carpel and axial ofructus T.Sinensis on the F2 generation of fertility

3 Conclusion and Discussion

The results of this study showed that with the increase of the concentration, the Chinese toon fruit petal medium shortened the pre-oviposition period, the larval stage and the pupal stage of the F1 and F2 generations of Drosophila; The pre-oviposition stage of the fly was extended first and then shortened, the larval stage was extended, and the pupal stage was shortened, and the degree continued to deepen with the increase of generations. In addition, under the same concentration conditions, the effects of the central axis of Toona sinensis on the pre-oviposition stage, larval stage, and pupal stage of the offspring Drosophila were greater than those of the fruit petals of Toona sinensis. There are differences in pharmacological effects. For the impact on the egg stage of the progeny fruit flies, except that the Chinese toon fruit petal medium of 2.5g/L concentration prolongs the egg stage of the F1 generation fruit flies, the Chinese toon fruit petals and the central axis of the other concentrations can not change the progeny In the egg stage of Drosophila, this may be due to the fact that the outer surface of the egg is covered by a layer of hard egg shell with high impermeability, making it difficult for external substances to penetrate into it and affect it.

The results of this experiment also showed that different concentrations of Chinese toon seed petals and medium axis medium could increase the number of offspring ♀ and ♂ fruit flies, but had little effect on the sex ratio of offspring ♀ and ♂ fruit flies (0.94-1.13 ). Through the comparative analysis of the number of offspring fruit flies, it was found that under the same concentration conditions, the proliferation rate of F1 and F2 generation ♀ and ♂ fruit flies in the axial medium was significantly higher than that of fruit petals. Therefore, it is speculated that the central axis and fruit petals of Toona sinensis contain active substances that can enhance the reproductive ability of fruit flies, and the effect of the central axis on promoting reproduction is better than that of the fruit petals. Through further analysis, it was found that the proliferation rate of the F2 generation of Drosophila was higher than that of the F1 generation, and the proliferation rate of the ♂ Drosophila was significantly higher than that of the ♀ Drosophila, but as the concentration increased, the F1, F2 generation ♀, ♂The number of fruit flies increased gradually.

Toona sinensis is widely distributed in my country, rich in resources, and rich in various active substances. At present, there are relatively few studies on its pharmacological activity, especially the biological activity analysis of toon fruit petals and central axis, especially on the physiological effects of Drosophila. Research has not been reported. Therefore, the results of this study show that there are significant differences in the effects of the fruit petals and central axes of Toona sinensis on the developmental duration and fecundity of the offspring Drosophila at different stages.

The composition of the invention can shorten the pre-oviposition period of fruit flies, contribute to mass reproduction of fruit flies, reduce feeding costs, improve feeding efficiency, and have beneficial significance for quickly providing a large number of fruit flies.

Drosophila salivary gland chromosomes are polytene chromosomes formed by continuous replication of dyed lines in salivary gland nuclei of Drosophila larvae but without nuclear division, also known as giant chromosomes. The composition of the invention can prolong the larval stage of fruit flies, help to increase the existence time of salivary gland chromosomes, increase the available time for the research on salivary gland chromosomes, and increase the operability in scientific research experiments or production.

The composition of the present invention can shorten the pupal period and help reduce the waiting time for studying other periods.

The composition of the invention can improve the fecundity of fruit flies, is beneficial to increase the breeding efficiency of fruit flies, and saves feed (medium).

The above embodiments are only used to further illustrate the present invention, and are not intended to limit the protection scope of the present invention. All equivalent transformations made based on the concept of the present invention and obvious improvements to the various technical solutions of the present invention all fall within the scope of the present invention. scope of protection.

Claims (10)

1. A composition, said composition contains the basal medium for Chinese Toona sinensis and cultivating Drosophila. 2. composition as claimed in claim 1, is characterized in that, The Chinese toon seed is a Chinese toon seed petal and/or the Chinese toon seed axis; and/or the fruit fly is Drosophila melanogaster. 3. compositions as claimed in claim 1, is characterized in that, The Chinese toon seed is Chinese toon seed powder. 4. compositions as claimed in claim 3, is characterized in that, The Chinese toon seed powder is the Chinese toon seed after passing through a 40 mesh sieve. 5. compositions as claimed in claim 1, is characterized in that, The basal medium for cultivating Drosophila contains: sucrose, agar, corn flour, propionic acid, yeast powder and water. 6. compositions as claimed in claim 5, is characterized in that, The weight ratio of the sucrose, the agar, the corn flour, the propionic acid, the yeast powder and the water is: 50-150:5-15:60-180:5-10:8-15 :1000, Preferably, the weight ratio of the sucrose, the agar, the corn flour, the propionic acid, the yeast powder and the water is: 31:3.1:41.3:2.5:3.5:380. 7. The composition of claim 1, wherein: The concentration of the Chinese toon seed in the basal medium for cultivating fruit flies is 1-100g/L, Preferably, the concentration of the Toona sinensis in the basal medium for cultivating Drosophila is 2.5g/L, 10g/L or 25g/L. 8. Use of the composition according to any one of claims 1-7 in improving the fecundity of Drosophila. 9. Use of the composition according to any one of claims 1-7 in shortening the pre-oviposition period and/or the pupal period of Drosophila. 10. Use of the composition according to any one of claims 1-7 for prolonging the larval stage of Drosophila.