CN104585495A - Preparation method for traditional Chinese medicine feed additive for reducing content of cholesterol in eggs - Google Patents
Preparation method for traditional Chinese medicine feed additive for reducing content of cholesterol in eggs Download PDFInfo
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- CN104585495A CN104585495A CN201510072279.9A CN201510072279A CN104585495A CN 104585495 A CN104585495 A CN 104585495A CN 201510072279 A CN201510072279 A CN 201510072279A CN 104585495 A CN104585495 A CN 104585495A
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Abstract
The invention discloses a preparation method for a traditional Chinese medicine feed additive for reducing content of cholesterol in eggs. The preparation method comprises the following steps: getting traditional Chinese medicines including hyodeoxycholic acid, grape seeds, Chinese chive seeds, smoked plums, cinnamon, mulberries, wild jujube seeds, garlic, clove and radix glycyrrhizae; performing superfine grinding on the traditional Chinese medicines, adding an alcohol solution and boiling with strong fire, preserving the heat with slow fire, cooling and filtering, concentrating the filtrate, adding white sugar and boiling, inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum after cooling to the room temperature, and fermenting for 168 hours to grow out white fungus blocks; performing vacuum-drying on the white fungus blocks at a low temperature and embedding the white fungus blocks, adding the white fungus blocks into feed in proportion, and pelletizing and cooling at a high temperature to prepare the granular feed. The traditional Chinese medicine feed additive has the effects of increasing quantity of effective microbial communities in the intestinal tract, improving the immune function and increasing the disease resistance of chicken bodies, and has the benefits of reducing the content of cholesterol in eggs. The preparation method is simple, content of live bacteria is high, use is convenient, no toxicity is realized and the cost is low.
Description
Technical field
The invention belongs to field of feed additive technology, relate to a kind of preparation method preventing and treating the natural green fowl Chinese herbal feed additive reducing cholesterol content in eggs specifically.
Background technology
Cholesterol (cholesterol) has another name called cholesterine, cholesterol, courage steroid-5-alkene-3 β-ol, and be a kind of steroidal compounds, chemical formula is C
27h
46o.A kind of white or off-white color flaky crystal under solid-state.Cholesterol is extensively present in animal body, and especially with the abundantest in brain and nerve fiber, in kidney, spleen, skin, liver and bile, content is also high.Its dissolubility and fats are seemingly, water insoluble, are slightly soluble in ethanol, methyl alcohol etc., are soluble in acetone, benzinum, ether, chloroform equal solvent.Cholesterol is the indispensable important substance of animal tissue cell, and it not only participates in forming cell membrane, and is synthetic bile acid, the important source material of vitamine D3 and cortex hormone of aadrenaline and sex hormone etc., and it is necessary to be that the mankind maintain normal physiological activity.
Cholesterol in blood of human body exists in two forms: CHF (accounting for 30%) and cholesteryl ester (accounting for 70%).Egg is one of people's wholefood of generally acknowledging, be also one of food of cholesterol enrichment, compared with the cholesterol deriving from other food, the cholesterol come from egg more easily causes the raising of human serum cholesterol levels.The normal cholesterol levels of different crowd is different.According to " Blood Lipid Abnormality in Chinese Adults guideline of prevention and treatment " that in May, 2010, China issued, China's adult's Blood total cholesterol levels delamination criterion is: be OK range lower than 200mg/dl, and 200 ~ 239mg/dl is that edge raises, higher than 240mg/dl for raising.The 4th Chinese residents in 2004 nutrition and Health Situation investigation result show, and the illness rate of China adult hypercholesterolemia is 2.9%, has people's cholesterolemia edge of 3.9% to raise in addition.
A large amount of medical research shows, the too high same artery sclerosis of cholesterol in serum level, cerebral thrombus and coronary heart disease etc. have close ties, the health of the serious threat mankind.According to statistics, caused because body inner cholesterol is too high more than 500 ten thousand people dead, and the crowd of 18% can be made to bring out angiocardiopathy.And cholesterol level often reduces by 1%, there is cardiopathic danger and just reduce by 2%.In recent years, along with Chinese society expanding economy and the raising of Living consumption and the acceleration of aging population, angiocardiopathy has become first cause of death of China city and rural crowd.
Researcher finds, the egg consuming more than 1 every day may make the danger of heart attack increase by 2%, and based on the quantity levels of people, this impact is very huge.Nutritionist advises being grown up the amount recommending to take in cholesterol every day should more than 300mg, to reduce the danger of hyperlipemia and coronary heart disease; For Patients with Cardiovascular/Cerebrovascular Diseases, should lower than 200mg.Therefore, provide the low-cholesterol egg of the applicable people particularly health that the elderly is edible significant.
At present, the method reducing cholesterol content in eggs is also more, some micro constitutents can be added in the daily ration of laying hen, as: copper, fish oil, allicin, chromium, medicine etc., these additives have the content of cholesterol in certain reduction egg, but also there are some problems: the range of decrease is about 15% as copper, fish oil etc., although consider very obvious from statistics, cholesterol level or a very high numerical value in egg; The effect that some additive reduces cholesterol is fine, but expensive, and such as HMGR inhibitor, generally can reduce by 40% ~ 50% by Cholesterol Content in Egg Yolk, mostly is people's medication, therefore makes feed cost increase; It is a lot of that some additive makes cholesterol level reduce, but egg production also reduces a lot simultaneously, as high doses of copper (250mg/kg).Su Zhenfang etc. disclose a kind of feed addictive [application number: 200710115302.3] reducing cholesterol content in eggs, rhizoma alismatis, marine alga, cassia seed, perilla seed, hawthorn, montmorillonite is added in feed, these composition great majority added belong to Chinese herbal medicine, and without any process, just pulverized 60 ~ 80 mesh sieves, added in feed by 2% ~ 6%, allowed chicken free choice feeding, according to the dietary habit of chicken, the effect that the content that may affect cholesterol in egg declines; Zhang Rude etc. disclose a kind of feed addictive [application number 200710009910.6] reducing cholesterol content in eggs, this invention contains to chicken feeding to the addition of allicin, anhydrous cupric sulfate, fish oil, natural red colouring agent for food, also used as a Chinese medicine feed, thus the content of cholesterol in reduction egg, this feed contains the materials such as fish oil because of it, egg may be made to contain certain fishy smell, and add cost, be not easy to realize industrialization; Wang Dengliang etc. disclose the feed reducing egg cholesterol and preparation method thereof [application number 201410373903.4], this invention be to chicken feed in full price basal diet increase green tea powder 7% ~ 8%, the content of fish meal is 2.5 ~ 5wt% and various trace elements, wherein Cu:0. 18 ~ 0.22g, the content about 15% of cholesterol in egg may be reduced, but the numerical value that in egg, cholesterol level is still very high.
And Chinese medicine is fermented together with microorganism be prepared into Chinese herbal feed additive, by probiotics through vacuum drying, and then through bilayered microcapsule embedding, make the feed addictive that can reduce cholesterol content in eggs, in prior art, have no report.
Summary of the invention
The object of the invention is to the deficiency for above technology, solve in current commercially available egg containing the problem compared with high cholesterol, there is provided a kind of and have the preparation method reducing cholesterol content in eggs Chinese herbal feed additive, simple, the easy to use, safe environmental protection of this preparation method, pure ecology, noresidue, viable bacteria content are high.
For achieving the above object, the preparation method that the present invention adopts is as follows:
Reduce a preparation method for cholesterol content in eggs Chinese herbal feed additive, it is characterized in that: it is prepared according to the following steps: 1, get Chinese medicine hyodesoxycholic acid, grape pip, leek seed, dark plum, Chinese cassia tree, mulberries, spina date seed, garlic, cloves, Radix Glycyrrhizae; 2, each Study on Supermicro-pulverization of Traditional Chinese Drugs is added ethanolic solution intense fire to boil, slow fire is incubated, cold filtration, and concentrated filtrate, adds white sugar and boil, and inoculating lactobacillus after normal temperature, Bayer Zygosaccharomyces bacterium and acetobacter xylinum are down in cooling, through 168h fermentation to growing white bacterium block; 3, by after white bacterium block low-temperature vacuum drying, embed, add in feed in proportion, through high temperature granulating cooling, obtained pellet.
Described a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive, is characterized in that: the Chinese medicine taking following weight portion: hyodesoxycholic acid 100 ~ 120, grape pip 100 ~ 120, leek seed 100 ~ 120, dark plum 80 ~ 100, Chinese cassia tree 80 ~ 100, mulberries 60 ~ 80, spina date seed 60 ~ 80, garlic 40 ~ 60, cloves 40 ~ 60, Radix Glycyrrhizae 20 ~ 30.
Described a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive, it is characterized in that: the Chinese medicine taking weight portion, ultramicro grinding, add the ethanolic solution of 50% ~ 70% of Chinese medicine gross weight 8 ~ 10 times amount, soak 30 ~ 60min, intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 6 ~ 8 times amount again, and intense fire boils, slow fire insulation 30 ~ 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector.
Described a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive, it is characterized in that: in units of 100 Kg concentrates, add white sugar 15 ~ 20Kg, and boil 5 ~ 10min, be cooled to normal temperature, inoculating lactobacillus, acetobacter xylinum, Bayer combining yeast bacterium (commercially available) preparation 10 ~ 15Kg, setting fermentation temperature 33 ~ 37 DEG C, humidity 33% ~ 40%, standing for fermentation cultivates 168h, to pH5.5 ~ 6.5 grow white bacterium block.
Described a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive, is characterized in that: take out white bacterium block, put into vacuum drying chamber, setting baking temperature 50 ~ 60 DEG C, drying time 8 ~ 12h, vacuum 0.08 ~ 0.1Mpa.
Described a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive, it is characterized in that: by dried white bacterium block after ultramicro grinding, embedding treatment is carried out with bilayered microcapsule, add in feed by 1% ~ 2%, through 80 ~ 90 DEG C, granulate 10 ~ 15s, cools and obtain pellet.
Chinese herbal feed additive of the present invention has increases profitable strain quantity in enteron aisle, improve immunologic function, strengthen the effects such as chicken body premunition, and preparation method is simple, easy to use, noresidue, viable bacteria content are high.Beneficial effect of the present invention is to reduce cholesterol in egg, improve essential amino acids content, through National agricultural portion, poultry quality supervision verification test Spot detection the results are shown in Table 1: cholesterol level 382mg/100g as can be seen from Table 1, than common egg 585mg/100g, reduce 34.70%, the percentage that aspartic acid, glutamic acid, leucine account for total amino acid total amount is respectively 11.77%, 12.34%, 10.71%, improves 2-3 doubly than three seed amino acids above-mentioned in common egg.
Table 1 assay report
The viable bacteria content of Chinese herbal feed additive beneficial bacterium of the present invention is high, asepticly takes 0.1g preparation, adds in 10ml dilution, fully shake up, make 10 times of serial dilutions, get last dilution factor 1ml, instill on selective agar culture plate, each dilution factor, make 3 plates, and with the coating of L shape glass rod evenly, put in constant incubator and cultivate 24 ~ 48h under 37 DEG C of conditions, cultivation expires and observes each plate colony growth situation, and counts.After testing after fermentation, after dry, high temperature do not embed and granulate after, the high temperature embedding viable bacteria content after rear, commercially available, commercially available high temperature granulating of granulating the results are shown in Table 2.
Table 2 viable bacteria content test results report
The rear liquid viable count content of fermentation is 2.2 × 10 as can be seen from Table 2
8cfu/g, after low-temperature vacuum drying, viable bacteria content is 2.4 × 10
10cfu/g, this is consistent with dried yield (recovery rate 10%), does not embed the viable count content after granulating 1.3 × 10 through high temperature
5cfu/g, suitable with viable count after commercially available high temperature granulating, the viable count after the high temperature granulating after embedding is 2.1 × 10
7cfu/g exceeds two orders of magnitude than the viable count do not embedded, and reaches every gram of viable bacteria more than millions of, thus, solve the problem of commercially available probiotics to the instability of high temperature.
Detailed description of the invention
embodiment 1
Reduce a preparation method for cholesterol content in eggs Chinese herbal feed additive, comprise the following steps:
Take the Chinese medicine of following weight portion (kg): hyodesoxycholic acid 100, grape pip 100, leek seed 100, dark plum 80, Chinese cassia tree 80, mulberries 60, spina date seed 60, garlic 40, cloves 40, Radix Glycyrrhizae 20.; Ultramicro grinding, adds the ethanolic solution of 50% of Chinese medicine gross weight 8 times amount, and soak 30min, intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 6 times amount again, and intense fire boils, slow fire insulation 30min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector.
The preparation of feed addictive: add white sugar 15Kg in units of 100 Kg concentrates, and boil 5min, be cooled to normal temperature, inoculating lactobacillus, acetobacter xylinum, Bayer combining yeast bacterium (commercially available) preparation 10Kg, setting fermentation temperature 33 DEG C, humidity 33%, standing for fermentation cultivates 168h, to pH5.5, till growing white bacterium block, put into vacuum drying chamber, setting baking temperature 50 DEG C, drying time 8h, vacuum 0.08Mpa, by dried white bacterium block after ultramicro grinding, embedding treatment is carried out with bilayered microcapsule, add in feed by 1%, through 80 DEG C, granulation 10s, cool and obtain pellet.
embodiment 2
Reduce a preparation method for cholesterol content in eggs Chinese herbal feed additive, comprise the following steps:
Take the Chinese medicine (except fresh pig's bile) of following weight portion (kg): hyodesoxycholic acid 110, grape pip 110, leek seed 110, dark plum 90, Chinese cassia tree 90, mulberries 70, spina date seed 70, garlic 50, cloves 50, Radix Glycyrrhizae 25.; Ultramicro grinding, adds the ethanolic solution of 60% of Chinese medicine gross weight 10 times amount, and soak 45min, intense fire boils, slow fire insulation 45min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 8 times amount again, and intense fire boils, slow fire insulation 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector.
The preparation of feed addictive: add white sugar 17.5Kg in units of 100 Kg concentrates, and boil 8min, be cooled to normal temperature, inoculating lactobacillus, acetobacter xylinum, Bayer combining yeast bacterium (commercially available) preparation 12.5Kg, setting fermentation temperature 35 DEG C, humidity 37%, standing for fermentation cultivates 168h, to pH6.0, till growing white bacterium block, put into vacuum drying chamber, setting baking temperature 55 DEG C, drying time 10h, vacuum 0.1Mpa, by dried white bacterium block after ultramicro grinding, embedding treatment is carried out with bilayered microcapsule, add in feed by 1.5%, through 85 DEG C, granulation 15s, cool and obtain pellet.
embodiment 3
Reduce a preparation method for cholesterol content in eggs Chinese herbal feed additive, comprise the following steps:
Take the Chinese medicine (except fresh pig's bile) of following weight portion (kg): hyodesoxycholic acid 120, grape pip 120, leek seed 120, dark plum 100, Chinese cassia tree 100, mulberries 80, spina date seed 80, garlic 60, cloves 60, Radix Glycyrrhizae 30.; Ultramicro grinding, adds the ethanolic solution of 70% of Chinese medicine gross weight 10 times amount, and soak 60min, intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 8 times amount again, and intense fire boils, slow fire insulation 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector.
The preparation of feed addictive: add white sugar 20Kg in units of 100 Kg concentrates, and boil 10min, be cooled to normal temperature, inoculating lactobacillus, acetobacter xylinum, Bayer combining yeast bacterium (commercially available) preparation 15Kg, setting fermentation temperature 37 DEG C, humidity 40%, standing for fermentation cultivates 168h, to pH6.5, till growing white bacterium block, put into vacuum drying chamber, setting baking temperature 60 DEG C, drying time 12h, vacuum 0.1Mpa, by dried white bacterium block after ultramicro grinding, embedding treatment is carried out with bilayered microcapsule, add in feed by 2%, through 90 DEG C, granulation 15s, cool and obtain pellet.
Claims (6)
1. reduce a preparation method for cholesterol content in eggs Chinese herbal feed additive, it is characterized in that: it is prepared according to the following steps: 1, get Chinese medicine hyodesoxycholic acid, grape pip, leek seed, dark plum, Chinese cassia tree, mulberries, spina date seed, garlic, cloves, Radix Glycyrrhizae; 2, each Study on Supermicro-pulverization of Traditional Chinese Drugs is added ethanolic solution intense fire to boil, slow fire is incubated, cold filtration, and concentrated filtrate, adds white sugar and boil, and inoculating lactobacillus after normal temperature, Bayer Zygosaccharomyces bacterium and acetobacter xylinum are down in cooling, through 168h fermentation to growing white bacterium block; 3, by after white bacterium block low-temperature vacuum drying, embed, add in feed in proportion, through high temperature granulating cooling, obtained pellet.
2. a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive according to claim 1, is characterized in that: the Chinese medicine taking following weight portion: hyodesoxycholic acid 100 ~ 120, grape pip 100 ~ 120, leek seed 100 ~ 120, dark plum 80 ~ 100, Chinese cassia tree 80 ~ 100, mulberries 60 ~ 80, spina date seed 60 ~ 80, garlic 40 ~ 60, cloves 40 ~ 60, Radix Glycyrrhizae 20 ~ 30.
3. a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive according to claim 1, it is characterized in that: the Chinese medicine taking weight portion, ultramicro grinding, add the ethanolic solution of 50% ~ 70% of Chinese medicine gross weight 8 ~ 10 times amount, soak 30 ~ 60min, intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 6 ~ 8 times amount again, and intense fire boils, slow fire insulation 30 ~ 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector.
4. a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive according to claim 1, it is characterized in that: in units of 100 Kg concentrates, add white sugar 15 ~ 20Kg, and boil 5 ~ 10min, be cooled to normal temperature, inoculating lactobacillus, acetobacter xylinum, Bayer combining yeast bacterium (commercially available) preparation 10 ~ 15Kg, setting fermentation temperature 33 ~ 37 DEG C, humidity 33% ~ 40%, standing for fermentation cultivates 168h, to pH5.5 ~ 6.5 grow white bacterium block.
5. a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive according to claim 1, it is characterized in that: take out white bacterium block, put into vacuum drying chamber, setting baking temperature 50 ~ 60 DEG C, drying time 8 ~ 12h, vacuum 0.08 ~ 0.1Mpa.
6. a kind of preparation method reducing cholesterol content in eggs Chinese herbal feed additive according to claim 1, it is characterized in that: by dried white bacterium block after ultramicro grinding, embedding treatment is carried out with bilayered microcapsule, add in feed by 1% ~ 2%, through 80 ~ 90 DEG C, granulate 10 ~ 15s, cools and obtain pellet.
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Cited By (3)
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| CN105995179A (en) * | 2016-07-05 | 2016-10-12 | 丁玉兰 | Preparing method for traditional Chinese medicine feed additive in laying hen growing period |
| CN106071308A (en) * | 2016-06-17 | 2016-11-09 | 高廷良 | A kind of feed additive producing low-cholesterol egg and preparation method thereof |
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