CN104686802A - Production method for low-cholesterol eggs - Google Patents
Production method for low-cholesterol eggs Download PDFInfo
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- CN104686802A CN104686802A CN201510071617.7A CN201510071617A CN104686802A CN 104686802 A CN104686802 A CN 104686802A CN 201510071617 A CN201510071617 A CN 201510071617A CN 104686802 A CN104686802 A CN 104686802A
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Abstract
The invention discloses a production method for low-cholesterol eggs. The production method is characterized by comprising the following steps: I, selecting a traditional Chinese medicine medicines which are used for reducing the absorption of chicken on cholesterol, strengthening the immunity of the chicken and increasing the nutrient ingredients according to a formula; II, fermenting the traditional Chinese medicines to obtain fermentation liquor for breeding laying hens; III, selecting laying hens, diluting the fermentation liquor in the step II in proportion for feeding the laying hens; and IV, obtaining eggs produced on an egg producing period of the fed laying hens to obtain low-cholesterol eggs. According to the production method disclosed by the invention, the content of the cholesterol in each egg is 156.2mg-181.6mg which is reduced by 42.2% in comparison with that of the cholesterol in each common egg, and the healthy low-cholesterol eggs are very suitable for being eaten by the old. The production method is suitable for large-scale breeding, easy to operate, convenient to use and low in cost.
Description
Technical field
The present invention relates to a kind of production method of egg, particularly relate to a kind of production method of low-cholesterol egg.
Background technology
Cholesterol (cholesterol) is extensively present in animal body, and especially with the abundantest in brain and nerve fiber, in kidney, spleen, skin, liver and bile, content is also high.Cholesterol is the indispensable important substance of animal tissue cell, and it not only participates in forming cell membrane, and is synthetic bile acid, the important source material of vitamine D3 and cortex hormone of aadrenaline and sex hormone etc., and it is necessary to be that the mankind maintain normal physiological activity.
Cholesterol in blood of human body exists in two forms: CHF (accounting for 30%) and cholesteryl ester (accounting for 70%).Egg is one of people's wholefood of generally acknowledging, be also one of food of cholesterol enrichment, compared with the cholesterol deriving from other food, the cholesterol come from egg more easily causes the raising of human serum cholesterol levels.A large amount of medical research shows, the too high same artery sclerosis of cholesterol in serum level, cerebral thrombus and coronary heart disease etc. have close ties, the health of the serious threat mankind.According to statistics, caused because body inner cholesterol is too high more than 500 ten thousand people dead, and the crowd of 18% can be made to bring out angiocardiopathy.
In recent years, along with Chinese society expanding economy and the raising of Living consumption and the acceleration of aging population, angiocardiopathy has become first cause of death of China city and rural crowd.Researcher finds, the egg consuming more than 1 every day may make the danger of heart attack increase by 2%, and based on the quantity levels of people, this impact is very huge.Nutritionist advises being grown up the amount recommending to take in cholesterol every day should more than 300mg, to reduce the danger of hyperlipemia and coronary heart disease; For Patients with Cardiovascular/Cerebrovascular Diseases, should lower than 200mg.Therefore, provide the low-cholesterol egg of the applicable people particularly health that the elderly is edible significant.
At present, the method reducing cholesterol content in eggs is also more, some micro constitutents can be added in the daily ration of laying hen, as: copper, fish oil, allicin, chromium, medicine etc., these additives have the content of cholesterol in certain reduction egg, but also there are some problems: the range of decrease is about 15% as copper, fish oil etc., although consider very obvious from statistics, cholesterol level or a very high numerical value in egg; The effect that some additive reduces cholesterol is fine, but expensive, and such as HMGR inhibitor, generally can reduce by 40% ~ 50% by Cholesterol Content in Egg Yolk, mostly is people's medication, therefore makes feed cost increase; It is a lot of that some additive makes cholesterol level reduce, but egg production also reduces a lot simultaneously, as high doses of copper (250mg/kg).Su Zhenfang etc. disclose a kind of feed addictive [application number: 200710115302.3] reducing cholesterol content in eggs, rhizoma alismatis, marine alga, cassia seed, perilla seed, hawthorn, montmorillonite is added in feed, these composition great majority added belong to Chinese herbal medicine, and without any process, just pulverized 60 ~ 80 mesh sieves, added in feed by 2% ~ 6%, allowed chicken free choice feeding, according to the dietary habit of chicken, the effect that the content that may affect cholesterol in egg declines; Zhang Rude etc. disclose a kind of feed addictive [application number 200710009910.6] reducing cholesterol content in eggs, this invention contains to chicken feeding to the addition of allicin, anhydrous cupric sulfate, fish oil, natural red colouring agent for food, also used as a Chinese medicine feed, thus the content of cholesterol in reduction egg, this feed contains the materials such as fish oil because of it, egg may be made to contain certain fishy smell, and add cost, be not easy to realize industrialization; Wang Dengliang etc. disclose the feed reducing egg cholesterol and preparation method thereof [application number 201410373903.4], this invention be to chicken feed in full price basal diet increase green tea powder 7% ~ 8%, the content of fish meal is 2.5 ~ 5wt% and various trace elements, wherein Cu:0. 18 ~ 0.22g, the content about 15% of cholesterol in egg may be reduced, but the numerical value that in egg, cholesterol level is still very high.
And Chinese medicine is fermented together with microorganism be prepared into Traditional Chinese drug microecology zymotic fluid, zymotic fluid is diluted in proportion relief chicken and freely drink, in prior art, have no report.
Summary of the invention
The object of the invention is to the deficiency for above technology, solve in current commercially available egg containing the problem compared with high cholesterol, there is provided a kind of production method with low-cholesterol egg, simple, the easy to use, safe environmental protection of this preparation method, pure ecology, noresidue.
For achieving the above object, the production method that the present invention adopts is as follows:
A production method for low-cholesterol egg, is characterized in that, step is as follows: one, the selection of Chinese prescription, and described Chinese medicine preparation, for reducing the absorption of chicken body to cholesterol, strengthens the immunocompetence of chicken body and the composition that has additional nutrients simultaneously; Two, above-mentioned Chinese prescription is fermented, obtain zymotic fluid, for raising laying hen; Three, select laying hen, utilize the zymotic fluid in step 2 to dilute in proportion and carry out raising laying hen; Four, by the laying hen after raising, the egg produced in laying period is low-cholesterol egg.
Further, described Chinese prescription is (by weight): hyodesoxycholic acid 100 ~ 120, grape pip 100 ~ 120, leek seed 100 ~ 120, dark plum 80 ~ 100, Chinese cassia tree 80 ~ 100, mulberries 60 ~ 80, spina date seed 60 ~ 80, garlic 40 ~ 60, cloves 40 ~ 60, Radix Glycyrrhizae 20 ~ 30.
Further, the preparation process of described zymotic fluid is as follows: one, take above-mentioned Chinese prescription, ultramicro grinding, add the ethanolic solution of 50% ~ 70% of Chinese medicine gross weight 8 ~ 10 times amount, soak 30 ~ 60min, intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 6 ~ 8 times amount again, and intense fire boils, slow fire insulation 30 ~ 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector; Two, in units of 100 Kg concentrates, add white sugar 15 ~ 20Kg, and boil 5 ~ 10min, be cooled to normal temperature; Three, the concentrate of above-mentioned interpolation white sugar is moved in aseptic fermenting cellar, inoculating lactobacillus, acetobacter xylinum, saccharomycete (commercially available) preparation 10 ~ 15Kg, setting fermentation temperature 33 ~ 37 DEG C, humidity 33% ~ 40%, standing for fermentation is cultivated 5 ~ 7 days, to pH5.5 ~ 6.5 grow white bacterium block, obtain Chinese medicine fermented liquid.
Further, described step 3 feeding process is: 9 ~ 20 weeks Growing Chicken, by zymotic fluid dilution 11 ~ 13 times, allows chicken freely drink; Within 21 ~ 28 weeks, lay eggs early stage, by zymotic fluid dilution 5 ~ 7 times, allow chicken freely drink; 29 ~ 71 weeks egg-laying peaks, by zymotic fluid dilution 2 ~ 4 times, allow chicken freely drink; After 72 weeks, by zymotic fluid dilution 3 ~ 5 times, allow chicken freely drink, its egg that produces is low-cholesterol egg.
Further, the egg that described step 4 is produced, in every piece of egg, the content of cholesterol is 159.2mg ~ 169.1mg.
Beneficial effect of the present invention is to reduce cholesterol in egg, improve essential amino acids content, through National agricultural standardization and monitoring center (Anhui) testing result in table 1: the percentage that aspartic acid, glutamic acid, leucine account for total amino acid total amount is as can be seen from Table 1 respectively 11.77%, 12.34%, 10.71%, improves 2-3 doubly than three seed amino acids above-mentioned in common egg.
Table 1 assay report
The detection of cholesterol in egg of the present invention, utilize high performance liquid chromatograph to detect, adopt external standard method to calculate the content of cholesterol, the egg before detection does following process: 1, saponification: take 100g egg, yolk is got in broken shell, in 500ml round-bottomed flask, add 300ml purified water, the NaOH of 10g, mixing, flask is placed in the heating jacket of magnetic agitation, agitating heating saponification backflow 12h, is cooled to normal temperature; 2, extract: the solution after saponification is transferred in 1000ml separatory funnel, add the vibration of 500ml benzinum, leave standstill 1h, separate petroleum ether layer, re-extract 3 times, combining extraction liquid; 3, concentrated: by above-mentioned extract, to be concentrated into dry in Rotary Evaporators, take out 0.1g anhydrous alcohol solution and be settled to 10ml, solution is crossed 0.45 μm of filter membrane and is filtered, and treats that chromatograph measures.Chromatographic condition: C18 chromatographic column; Column temperature: 40 DEG C; Mobile phase: methyl alcohol; Flow velocity: 1.0ml/min; Measure wavelength: 210nm; Sample size 10 μ l.The result calculated by external standard method is as follows: cholesterol content in eggs of the present invention is 318.5 ~ 338.2mg/100g, commercially available cholesterol content in eggs is 585.6mg/100g, and in the egg that the present invention produces compared with common commercially available egg, the content of cholesterol reduces 42.2 ~ 45.6%.
Accompanying drawing explanation
Fig. 1 cholesterol standards collection of illustrative plates.
Fig. 2 lay eggs early stage egg in cholesterol collection of illustrative plates.
Cholesterol collection of illustrative plates in Fig. 3 laying period egg.
Cholesterol collection of illustrative plates in the commercially available egg of Fig. 4.
Detailed description of the invention
embodiment 1
A production method for low-cholesterol egg, step is as follows: one, the selection of Chinese prescription, and described Chinese medicine preparation, for reducing the absorption of chicken body to cholesterol, strengthens the immunocompetence of chicken body and the composition that has additional nutrients simultaneously; Two, above-mentioned Chinese prescription is fermented, obtain zymotic fluid, for raising laying hen; Three, select laying hen, utilize the zymotic fluid in step 2 to dilute in proportion and carry out raising laying hen; Four, by the laying hen after raising, the egg produced in laying period is low-cholesterol egg.
Further, described Chinese prescription is (by weight): hyodesoxycholic acid 100, grape pip 100, leek seed 100, dark plum 80, Chinese cassia tree 80, mulberries 60, spina date seed 60, garlic 40, cloves 40, Radix Glycyrrhizae 20.
Further, the preparation process of described zymotic fluid is as follows: one, take above-mentioned Chinese prescription, ultramicro grinding, adds the ethanolic solution of 50% of Chinese medicine gross weight 8 times amount, and soak 30min, intense fire boils, slow fire insulation 300min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 6 times amount again, and intense fire boils, slow fire insulation 30min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector; Two, in units of 100 Kg concentrates, add white sugar 15Kg, and boil 5min, be cooled to normal temperature; Three, the concentrate of above-mentioned interpolation white sugar is moved in aseptic fermenting cellar, inoculating lactobacillus, acetobacter xylinum, saccharomycete (commercially available) preparation 10Kg, setting fermentation temperature 33 DEG C, humidity 33%, standing for fermentation cultivates 5 days, to pH5.5 grows white bacterium block, obtains Chinese medicine fermented liquid.
The present embodiment selects the numb chicken in north, Anhui as laying hen.
Further, described step 3 feeding process is: 9 ~ 20 weeks Growing Chicken, zymotic fluid is diluted 11 times, allows chicken freely drink; Within 21 ~ 28 weeks, lay eggs early stage, zymotic fluid is diluted 5 times, allow chicken freely drink; 29 ~ 71 weeks egg-laying peaks, dilute 2 times by zymotic fluid, allow chicken freely drink; After 72 weeks, zymotic fluid is diluted 3 times, allow chicken freely drink, its egg that produces is low-cholesterol egg.
Further, the egg that described step 4 is produced, in every piece of egg, the content of cholesterol is 159.2mg.
embodiment 2
A production method for low-cholesterol egg, step is as follows: one, the selection of Chinese prescription, and described Chinese medicine preparation, for reducing the absorption of chicken body to cholesterol, strengthens the immunocompetence of chicken body and the composition that has additional nutrients simultaneously; Two, above-mentioned Chinese prescription is fermented, obtain zymotic fluid, for raising laying hen; Three, select laying hen, utilize the zymotic fluid in step 2 to dilute in proportion and carry out raising laying hen; Four, by the laying hen after raising, the egg produced in laying period is low-cholesterol egg.
Further, described Chinese prescription is (by weight): hyodesoxycholic acid 110, grape pip 110, leek seed 110, dark plum 90, Chinese cassia tree 90, mulberries 70, spina date seed 70, garlic 50, cloves 50, Radix Glycyrrhizae 25.
Further, the preparation process of described zymotic fluid is as follows: one, take above-mentioned Chinese prescription, ultramicro grinding, adds the ethanolic solution of 60% of Chinese medicine gross weight 9 times amount, and soak 45min, intense fire boils, slow fire insulation 45min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 8 times amount again, and intense fire boils, slow fire insulation 45min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector; Two, in units of 100 Kg concentrates, add white sugar 20Kg, and boil 10min, be cooled to normal temperature; Three, the concentrate of above-mentioned interpolation white sugar is moved in aseptic fermenting cellar, inoculating lactobacillus, acetobacter xylinum, saccharomycete (commercially available) preparation 15Kg, setting fermentation temperature 35 DEG C, humidity 37%, standing for fermentation cultivates 6 days, to pH6.0 grows white bacterium block, obtains Chinese medicine fermented liquid.
The present embodiment selects Luo Man brown shell layer as laying hen.
Further, described step 3 feeding process is: 9 ~ 20 weeks Growing Chicken, zymotic fluid is diluted 12 times, allows chicken freely drink; Within 21 ~ 28 weeks, lay eggs early stage, zymotic fluid is diluted 6 times, allow chicken freely drink; 29 ~ 71 weeks egg-laying peaks, dilute 3 times by zymotic fluid, allow chicken freely drink; After 72 weeks, zymotic fluid is diluted 4 times, allow chicken freely drink, its egg that produces is low-cholesterol egg.
Further, the egg that described step 4 is produced, in every piece of egg, the content of cholesterol is 169.1mg.
embodiment 3
A production method for low-cholesterol egg, step is as follows: one, the selection of Chinese prescription, and described Chinese medicine preparation, for reducing the absorption of chicken body to cholesterol, strengthens the immunocompetence of chicken body and the composition that has additional nutrients simultaneously; Two, above-mentioned Chinese prescription is fermented, obtain zymotic fluid, for raising laying hen; Three, select laying hen, utilize the zymotic fluid in step 2 to dilute in proportion and carry out raising laying hen; Four, by the laying hen after raising, the egg produced in laying period is low-cholesterol egg.
Further, described Chinese prescription is (by weight): hyodesoxycholic acid 120, grape pip 120, leek seed 120, dark plum 100, Chinese cassia tree 100, mulberries 80, spina date seed 80, garlic 60, cloves 60, Radix Glycyrrhizae 30.
Further, the preparation process of described zymotic fluid is as follows: one, take above-mentioned Chinese prescription, ultramicro grinding, adds the ethanolic solution of 70% of Chinese medicine gross weight 10 times amount, and soak 60min, intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 8 times amount again, and intense fire boils, slow fire insulation 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector; Two, in units of 100 Kg concentrates, add white sugar 20Kg, and boil 10min, be cooled to normal temperature; Three, the concentrate of above-mentioned interpolation white sugar is moved in aseptic fermenting cellar, inoculating lactobacillus, acetobacter xylinum, saccharomycete (commercially available) preparation 15Kg, setting fermentation temperature 37 DEG C, humidity 40%, standing for fermentation cultivates 7 days, to pH6.5 grows white bacterium block, obtains Chinese medicine fermented liquid.
The present embodiment selects Leghorn as laying hen.
Further, described step 3 feeding process is: 9 ~ 20 weeks Growing Chicken, zymotic fluid is diluted 13 times, allows chicken freely drink; Within 21 ~ 28 weeks, lay eggs early stage, zymotic fluid is diluted 7 times, allow chicken freely drink; 29 ~ 71 weeks egg-laying peaks, dilute 4 times by zymotic fluid, allow chicken freely drink; After 72 weeks, zymotic fluid is diluted 5 times, allow chicken freely drink, its egg that produces is low-cholesterol egg.
Further, the egg that described step 4 is produced, in every piece of egg, the content of cholesterol is 165.6mg.
Claims (5)
1. a production method for low-cholesterol egg, is characterized in that, step is as follows: one, the selection of Chinese prescription, and described Chinese medicine preparation, for reducing the absorption of chicken body to cholesterol, strengthens the immunocompetence of chicken body and the composition that has additional nutrients simultaneously; Two, above-mentioned Chinese prescription is fermented, obtain zymotic fluid, for raising laying hen; Three, select laying hen, utilize the zymotic fluid in step 2 to dilute in proportion and carry out raising laying hen; Four, by the laying hen after raising, the egg produced in laying period is low-cholesterol egg.
2. the production method of a kind of low-cholesterol egg according to claim 1, is characterized in that: described Chinese prescription is (by weight): hyodesoxycholic acid 100 ~ 120, grape pip 100 ~ 120, leek seed 100 ~ 120, dark plum 80 ~ 100, Chinese cassia tree 80 ~ 100, mulberries 60 ~ 80, spina date seed 60 ~ 80, garlic 40 ~ 60, cloves 40 ~ 60, Radix Glycyrrhizae 20 ~ 30.
3. the production method of a kind of low-cholesterol egg according to claim 1, it is characterized in that: the preparation process of described zymotic fluid is as follows: one, take above-mentioned Chinese prescription, ultramicro grinding, add the ethanolic solution of 50% ~ 70% of Chinese medicine gross weight 8 ~ 10 times amount, soak 30 ~ 60min, intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze; Filter residue adds the water of 6 ~ 8 times amount again, and intense fire boils, slow fire insulation 30 ~ 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detects in concentrate concentrated without stopping during ethanol with ethanol detector; Two, in units of 100 Kg concentrates, add white sugar 15 ~ 20Kg, and boil 5 ~ 10min, be cooled to normal temperature; Three, the concentrate of above-mentioned interpolation white sugar is moved in aseptic fermenting cellar, inoculating lactobacillus, acetobacter xylinum, saccharomycete (commercially available) preparation 10 ~ 15Kg, setting fermentation temperature 33 ~ 37 DEG C, humidity 33% ~ 40%, standing for fermentation is cultivated 5 ~ 7 days, to pH5.5 ~ 6.5 grow white bacterium block, obtain Chinese medicine fermented liquid.
4. the production method of a kind of low-cholesterol egg according to claim 1, is characterized in that: described step 3 feeding process is: 9 ~ 20 weeks Growing Chicken, by zymotic fluid dilution 11 ~ 13 times, allows chicken freely drink; Within 21 ~ 28 weeks, lay eggs early stage, by zymotic fluid dilution 5 ~ 7 times, allow chicken freely drink; 29 ~ 71 weeks egg-laying peaks, by zymotic fluid dilution 2 ~ 4 times, allow chicken freely drink; After 72 weeks, by zymotic fluid dilution 3 ~ 5 times, allow chicken freely drink, its egg that produces is low-cholesterol egg.
5. the production method of a kind of low-cholesterol egg according to claim 1, it is characterized in that: the egg that described step 4 is produced, in every piece of egg, the content of cholesterol is 159.2mg ~ 169.1mg.
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| CN106071308A (en) * | 2016-06-17 | 2016-11-09 | 高廷良 | A kind of feed additive producing low-cholesterol egg and preparation method thereof |
| CN106259186A (en) * | 2016-08-17 | 2017-01-04 | 咸宁市农业科学院 | A kind of production method of low-cholesterol egg |
| CN109744193A (en) * | 2017-11-08 | 2019-05-14 | 天津硒艾格生物科技有限公司 | A kind of production method of low cholesterol high linoleic acid egg |
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