CN109007316A - A kind of NEW TYPE OF COMPOSITE protein fermentation feed and preparation method thereof - Google Patents
A kind of NEW TYPE OF COMPOSITE protein fermentation feed and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of preparation method of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps: step 1, the tealeaf residue of the dregs of beans of 10%-30% and 70%-90% is mixed, compound protein raw material is obtained；Step 2, compound protein raw material is mixed with water by the weight ratio of 1:05-1.5, obtains compound protein mixture；Step 3, composite zymocyte liquid is inoculated with the compound protein mixture after sterilizing by the inoculum concentration of 5%-20%, after mixing, charges material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 28 DEG C -35 DEG C static fermentation 2 days or more；Wherein, the composite zymocyte liquid is made of the mixing of bacillus subtilis bacterium solution, saccharomycopsis fibuligera bacterium solution and Bacillus acidi lactici liquid.The present invention also provides NEW TYPE OF COMPOSITE protein fermentation feeds prepared by the preparation method.The present invention can reduce tealeaf residue and waste residue pollution on the environment, additionally it is possible to alleviate the status of protein feed resources shortage significantly.
Description
Technical field
The present invention relates to biological fermentation feed manufacture field more particularly to a kind of NEW TYPE OF COMPOSITE protein fermentation feed feed and
Preparation method.
Background technique
With the fast development of China's breeding scale industry, feed resource shortage problem has been got worse, especially protein
Feed resource shortage problem is more troubling.It is analyzed according to the relevant expert of Institute of Feeds,China Academy of Agriculture Sciences, to 2020
Year, China's protein feed year insufficiency of supply-demand will be up to 48,000,000 tons.Therefore it depends merely on and utilizes existing conventional plant protein raw materials (such as beans
The dregs of rice, rapeseed dregs etc.) and animal protein raw material, it is difficult to meet the needs of fast-developing aquaculture.Therefore, how according to state of China
Feelings expand existing feed resource, develop and promoted unconventional or low value-added protein feed resources, to alleviate the feeding of China's albumen
Material shortage of resources status has become a top priority.
" the tealeaves industry production and marketing demand and investment forecasting analysis report " budget issued according to prediction industrial research institute, 2017
China's tea yield is up to 2,580,000 tons, it is contemplated that arrives the year two thousand twenty, China's tea yield will be up to 3,340,000 tons.With swashing for tea yield
Increase, large quantities of tea comprehensive processing enterprises come into being；And tealeaves dosage 70% can be generated during tea comprehensive processing industrialization production
Above tealeaf residue.Studies show that, in tealeaf residue containing 17~19% crude protein, 16~18% crude fibre, 1~2%
Tea polyphenols, 0.1~0.3% caffeine.Since tealeaf residue contains a large amount of nutritional ingredient, and moisture content is higher, easily breeds
A large amount of microorganism directly abandons and easily causes environmental pollution and the wasting of resources.Therefore the research of tealeaf residue resource utilization is more next
More it is concerned by people.
As a kind of novel fodder, early in the 1960s and 1970s, someone studies tealeaf residue；But since China passes
System cultivates the low of processing technology, this excellent feed of tealeaf residue is not developed and used always well.Therefore, how more preferably
Ground develops and uses tealeaf residue, becomes a kind of compound protein fermented feed, will have good market prospects.
Summary of the invention
The technical problems to be solved by the present invention are: in view of the drawbacks of the prior art, providing a kind of NEW TYPE OF COMPOSITE albumen hair
Ferment feed feed and preparation method thereof directly utilizes microbial fermentation technology, cooperates certain nutrition to compound control technique, by tea
Leaf slag is directly developed into a kind of compound protein fermented feed, and the dirt caused by environment of tealeaf residue and waste residue can be not only greatly reduced
Dye, moreover it is possible to alleviate the status of protein feed resources shortage significantly, to reduce Production of Livestock and Poultry cost, and then it is competing to improve product market
Strive power.
The present invention uses following technical scheme to solve above-mentioned technical problem:
The present invention provides a kind of preparation method of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
Step 1, the tealeaf residue of the dregs of beans of 10%-30% and 70%-90% are mixed, obtains compound protein raw material；
Step 2, compound protein raw material is mixed with water by the weight ratio of 1:0.5-1.5, obtains compound protein mixing
Material, and sterilization treatment is carried out to compound protein mixture；
Step 3, bacillus subtilis bacterium solution, saccharomycopsis fibuligera bacterium solution and Bacillus acidi lactici liquid are mixed with composite fermentation
Bacterium solution；
Step 4, composite zymocyte liquid connects the compound protein mixture after sterilizing by the inoculum concentration of 5%-20%
Kind, after mixing, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 28 DEG C -35 DEG C static fermentations 2
More than it, the NEW TYPE OF COMPOSITE protein fermentation feed is obtained.
In order to optimize above-mentioned technical solution, the technical measures that the present invention takes further include:
Further, in step 1, the compound protein raw material is by the dregs of beans of 15%-25% and the tealeaf residue of 75%-85%
It is mixed to get.
Further, in step 2, the compound protein raw material is mixed with water by the weight ratio of 1:0.5-1.0, mixing
Raw material pH 7-8 afterwards.
Further, bacillus subtilis bacterium solution, saccharomycopsis fibuligera bacterium solution and lactic acid bar in the composite zymocyte liquid
The weight percent of bacterium solution are as follows:
Bacillus subtilis bacterium solution 30%
Saccharomycopsis fibuligera bacterium solution 30%
Bacillus acidi lactici liquid 40%.
Further, the bacillus subtilis bacterium solution is purchased from Chinese agriculture Microbiological Culture Collection administrative center, and preservation is compiled
Number be ACCC11025；The saccharomycopsis fibuligera is purchased from Chinese agriculture Microbiological Culture Collection administrative center, and deposit number is
ACCC20015；The Bacillus acidi lactici is purchased from Chinese agriculture Microbiological Culture Collection administrative center, deposit number ACCC11026.
Further, the bacillus subtilis bacterium solution the preparation method comprises the following steps: bacillus subtilis is accessed bacillus subtilis
In bacterium culture medium, 7.0,35 DEG C of initial pH, 200r/min 24~36h of shaken cultivation obtain bacillus subtilis bacterium solution；It is described withered
Careless bacillus culture medium component is as follows: glucose 25.0g/L, peptone 5.0g/L, beef extract 3.0g/L, yeast extract
1.0g/L, MgS04·7H20 0.5g/L, MnS04·H20 0.005g/L, water are settled to 1L.
Further, the saccharomycopsis fibuligera bacterium solution the preparation method comprises the following steps: by saccharomycopsis fibuligera access button capsule
In laminating adhesive spore yeast culture medium, initial pH 5.5,30-32 DEG C, 200r/min shaken cultivation for 24 hours, obtains saccharomycopsis fibuligera
Bacterium solution；The saccharomycopsis fibuligera nutrient media components are as follows: glucose 45.0g/L, yeast extract 12.0g/L, potassium dihydrogen phosphate
0.8g/L, magnesium sulfate 0.5g/L, water are settled to 1L.
Further, the Bacillus acidi lactici liquid the preparation method comprises the following steps: by Bacillus acidi lactici access Bacillus acidi lactici culture medium in, pH
Naturally, 36 DEG C of culture 40h, obtain Bacillus acidi lactici liquid；The Bacillus acidi lactici nutrient media components are as follows: glucose 30.0g/L, albumen
Peptone 15.0g/L, yeast extract 15.0g/L, KH2PO41.0g/L, MnS04·H20 0.0001g/L, CaCO30.3g/L, water constant volume
To 1L.
Further, in step 3, total bacteria count is greater than 10 in the composite zymocyte liquid8cfu/g。
Further, in step 3, the composite zymocyte liquid is by the inoculum concentration of 8%-12% to the compound protein after sterilizing
Mixture is inoculated with.
Further, in step 3, the time of static fermentation is preferably 2-3 days.
The present invention provides a kind of NEW TYPE OF COMPOSITE protein fermentation feed prepared by the preparation method.
The present invention by adopting the above technical scheme, compared with prior art, has the following technical effect that
1) present invention is by using microbial fermentation technology, and certain nutrition is cooperated to compound control technique, palatability is poor,
The tealeaf residue that tradition is dropped be developed into palatability is good, crude fiber content is low, crude protein content be up to 25% or more high egg
White feed can not only greatly reduce tealeaf residue and waste residue pollution on the environment, moreover it is possible to alleviation protein feed resources significantly
The status of shortage to reduce Production of Livestock and Poultry cost, and then improves product competitiveness in the market.
2) the NEW TYPE OF COMPOSITE protein fermentation feed that the present invention is prepared can not only greatly improve crude protein in tealeaf residue
Content, moreover it is possible to increase the content of a variety of digestive ferments, organic acid, functional microbial, polypeptide and amino acid, there is significant growth-promoting
Long, aid digestion, anti-disease increases daily gain, reduces the effects of feedstuff-meat ratio, environment purification.
3) preparation method simple production process of the invention, the production cycle is shorter, is conducive to industrialized production.
Specific embodiment
Technical solution of the present invention is described in further detail below with reference to embodiment.Following embodiment is only used for more
Add and clearly demonstrate technical solution of the present invention, and not intended to limit the protection scope of the present invention.
Embodiment 1
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
The preparation of bacillus subtilis (ACCC11025) seed liquor: by bacillus subtilis access bacillus subtilis training
It supports in base, 7.0,35 DEG C of initial pH, 24~36h of 200r/min shaken cultivation；Bacillus subtilis nutrient media components are as follows: Portugal
Grape sugar 25.0g/L, peptone 5.0g/L, beef extract 3.0g/L, yeast extract 1.0g/L, MgS04·7H20 0.5g/L,
MnS04·H20 0.005g/L, water are settled to 1L；
The preparation of saccharomycopsis fibuligera (ACCC20015) seed liquor: saccharomycopsis fibuligera is accessed into button capsule laminating adhesive spore
In yeast culture medium, initial pH 5.5,30-32 DEG C, 200r/min shaken cultivation is for 24 hours；Saccharomycopsis fibuligera nutrient media components
As follows: glucose 45.0g/L, yeast extract 12.0g/L, potassium dihydrogen phosphate 0.8g/L, magnesium sulfate 0.5g/L, water are settled to 1L；
The preparation of Bacillus acidi lactici (ACCC11026) seed liquor: Bacillus acidi lactici is accessed in Bacillus acidi lactici culture medium, and pH is certainly
So, 36 DEG C of culture 40h, Bacillus acidi lactici nutrient media components are as follows: glucose 30.0g/L, peptone 15.0g/L, yeast extract
15.0g/L KH2PO41.0g/L, MnS04·H20 0.0001g/L, CaCO30.3g/L, water are settled to 1L.
2) preparation of composite zymocyte liquid
Bacillus subtilis (ACCC11025) that step 1) is prepared, saccharomycopsis fibuligera (ACCC20015),
Bacillus acidi lactici (ACCC11026) seed liquor is mixed by the weight ratio of 30%:30%:40%, and composite zymocyte liquid is made, multiple
It closes viable count in zymocyte liquid and is greater than 108cfu/g。
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 90% weight ratio by 10%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.5, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 5% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 28 DEG C static fermentation 3 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 2
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 90% weight ratio by 10%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.8, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 7% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 3
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 85% weight ratio by 15%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:1.0, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 5% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 4
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 85% weight ratio by 15%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.5, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 10% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 5
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 80% weight ratio by 20%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.5, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 10% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 6
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 80% weight ratio by 20%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.7, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 7% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 7
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 75% weight ratio by 25%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.6, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 8% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 8
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 75% weight ratio by 25%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.5, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 10% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 28 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 9
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 70% weight ratio by 30%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:0.8, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 7% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 10
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 80% weight ratio by 20%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:1.5, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 12% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 30 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Embodiment 11
The present embodiment provides a kind of preparation methods of NEW TYPE OF COMPOSITE protein fermentation feed, comprising the following steps:
1) preparation of strain
Step is the same as embodiment 1
2) preparation of composite zymocyte liquid
Step is the same as embodiment 1
3) preparation of compound protein fermented feed
The dregs of beans and tealeaf residue of 40 meshes will be crushed, is uniformly mixed, is obtained with 70% weight ratio by 30%
Compound protein raw material；Compound protein raw material is mixed with water by the weight ratio of 1:1.0, material pH is naturally, mixed
Material uses steam pressure 1atm to mixture, and 121 DEG C of temperature, the autoclaving of time 20min carries out sterilization treatment；
The composite zymocyte liquid that step 2) is prepared is inoculated with the mixture after sterilizing by weight 20% inoculum concentration, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 35 DEG C static fermentation 2 days, that is, be made
NEW TYPE OF COMPOSITE protein fermentation feed.
Verification experimental verification
The present embodiment measures it to the NEW TYPE OF COMPOSITE protein fermentation feed being prepared in embodiment 1~11 and as former state respectively
Crude protein and coarse-fibred content, and calculate its crude protein growth rate and lignocellulose degradation rate, concrete outcome are detailed in the following table 1:
Comparativeanalysis of the 1 NEW TYPE OF COMPOSITE protein feed of table before and after fermentation
By above-mentioned table 1, it can be seen that, the preparation method of NEW TYPE OF COMPOSITE protein fermentation feed of the invention is sent out with microorganism
Ferment technology compounds control technique with nutrition, carries out fermentation process, the fermentation produced to the mixed raw material of tealeaf residue and dregs of beans
Crude protein content is expected between 25%~39%, and albumen growth rate is between 35%~62%；Crude fiber content 12%~
Between 18%, lignocellulose degradation rate is between 18%~38%.Illustrate that the present invention can not only greatly reduce tealeaf residue and waste residue
Pollution on the environment really realizes " the turning waste into wealth " of resource；The status that protein feed resources shortage can also be alleviated, from
And Production of Livestock and Poultry cost is reduced, and then promote product competitiveness in the market.
Specific embodiments of the present invention are described in detail above, but it is only used as example, the present invention is not intended to limit
In particular embodiments described above.To those skilled in the art, it any equivalent modifications to the practical progress and replaces
In generation, is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by equal transformation and repair
Change, all should be contained within the scope of the invention.
Claims (10)
1. a kind of preparation method of NEW TYPE OF COMPOSITE protein fermentation feed, which comprises the following steps:
Step 1, the tealeaf residue of the dregs of beans of 10%-30% and 70%-90% are mixed, obtains compound protein raw material；
Step 2, compound protein raw material is mixed with water by the weight ratio of 1:0.5-1.5, obtains compound protein mixture, and
Sterilization treatment is carried out to compound protein mixture；
Step 3, bacillus subtilis bacterium solution, saccharomycopsis fibuligera bacterium solution and Bacillus acidi lactici liquid are mixed with composite zymocyte
Liquid；
Step 4, composite zymocyte liquid is inoculated with the compound protein mixture after sterilizing by the inoculum concentration of 5%-20%, mixes
After closing uniformly, charge material into the packaging bag hermetic package with unidirectional gas outlet valve apparatus, 28 DEG C -35 DEG C static fermentations 2 days with
On, obtain the NEW TYPE OF COMPOSITE protein fermentation feed.
2. preparation method according to claim 1, which is characterized in that in step 1, the compound protein raw material is by 15%-
25% dregs of beans and the tealeaf residue of 75%-85% are mixed to get.
3. preparation method according to claim 1, which is characterized in that in step 2, the compound protein raw material and water press 1:
The weight ratio of 0.5-1.0 is mixed, raw material pH 7-8 after mixing.
4. preparation method according to claim 1, which is characterized in that in step 3, withered grass bud in the composite zymocyte liquid
The weight percent of spore bacillus liquid, saccharomycopsis fibuligera bacterium solution and Bacillus acidi lactici liquid are as follows:
Bacillus subtilis bacterium solution 30%
Saccharomycopsis fibuligera bacterium solution 30%
Bacillus acidi lactici liquid 40%.
5. preparation method according to claim 1, which is characterized in that in step 3, the preparation of the bacillus subtilis bacterium solution
Method are as follows: access bacillus subtilis in bacillus subtilis bacterium culture medium, 7.0,35 DEG C of initial pH, 200r/min oscillation training
24~36h is supported, bacillus subtilis bacterium solution is obtained；The bacillus subtilis nutrient media components are as follows: glucose 25.0g/L, egg
White peptone 5.0g/L, beef extract 3.0g/L, yeast extract 1.0g/L, MgS04·7H20 0.5g/L, MnS04·H20 0.005g/L,
Water is settled to 1L.
6. preparation method according to claim 1, which is characterized in that in step 3, the saccharomycopsis fibuligera bacterium solution
The preparation method comprises the following steps: saccharomycopsis fibuligera is accessed in saccharomycopsis fibuligera culture medium, initial pH 5.5,30-32 DEG C,
200r/min shaken cultivation for 24 hours, obtains saccharomycopsis fibuligera bacterium solution；The saccharomycopsis fibuligera nutrient media components are as follows:
Glucose 45.0g/L, yeast extract 12.0g/L, potassium dihydrogen phosphate 0.8g/L, magnesium sulfate 0.5g/L, water are settled to 1L.
7. preparation method according to claim 1, which is characterized in that in step 3, the preparation method of the Bacillus acidi lactici liquid
Are as follows: Bacillus acidi lactici is accessed in Bacillus acidi lactici culture medium, pH obtains Bacillus acidi lactici liquid naturally, 36 DEG C of culture 40h；The lactic acid
Baccilus medium component is as follows: glucose 30.0g/L, peptone 15.0g/L, yeast extract 15.0g/L, KH2PO41.0g/L
MnS04·H20 0.0001g/L, CaCO30.3g/L, water are settled to 1L.
8. preparation method according to claim 1, which is characterized in that in step 3, total bacteria count in the composite zymocyte liquid
Greater than 108cfu/g。
9. preparation method according to claim 1, which is characterized in that in step 4, the composite zymocyte liquid presses 8%-
12% inoculum concentration is inoculated with the compound protein mixture after sterilizing.
10. a kind of NEW TYPE OF COMPOSITE protein fermentation feed prepared such as any one of claim 1-9 preparation method.
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