CN108998444A - 一种转移液体式核酸提取装置及方法 - Google Patents
一种转移液体式核酸提取装置及方法 Download PDFInfo
- Publication number
- CN108998444A CN108998444A CN201810928530.0A CN201810928530A CN108998444A CN 108998444 A CN108998444 A CN 108998444A CN 201810928530 A CN201810928530 A CN 201810928530A CN 108998444 A CN108998444 A CN 108998444A
- Authority
- CN
- China
- Prior art keywords
- sample
- deep
- well plates
- carrier
- magnetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 49
- 238000012546 transfer Methods 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 26
- 230000007246 mechanism Effects 0.000 claims abstract description 111
- 238000010438 heat treatment Methods 0.000 claims abstract description 58
- 238000000605 extraction Methods 0.000 claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 36
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052782 aluminium Inorganic materials 0.000 claims abstract description 25
- 230000000149 penetrating effect Effects 0.000 claims abstract description 18
- 238000005057 refrigeration Methods 0.000 claims abstract description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 17
- 230000009514 concussion Effects 0.000 claims abstract description 15
- 239000004065 semiconductor Substances 0.000 claims abstract description 8
- 238000012360 testing method Methods 0.000 claims description 17
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 230000001360 synchronised effect Effects 0.000 claims description 15
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 14
- 230000003028 elevating effect Effects 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 11
- 230000003287 optical effect Effects 0.000 claims description 7
- 230000002070 germicidal effect Effects 0.000 claims description 6
- 239000011324 bead Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000002474 experimental method Methods 0.000 claims description 3
- 239000004411 aluminium Substances 0.000 claims 1
- 238000011534 incubation Methods 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 11
- 230000000694 effects Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 101100493714 Caenorhabditis elegans bath-47 gene Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000005679 Peltier effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810928530.0A CN108998444B (zh) | 2018-08-15 | 2018-08-15 | 一种转移液体式核酸提取装置及方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810928530.0A CN108998444B (zh) | 2018-08-15 | 2018-08-15 | 一种转移液体式核酸提取装置及方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108998444A true CN108998444A (zh) | 2018-12-14 |
CN108998444B CN108998444B (zh) | 2023-11-21 |
Family
ID=64592958
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810928530.0A Active CN108998444B (zh) | 2018-08-15 | 2018-08-15 | 一种转移液体式核酸提取装置及方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108998444B (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108048302A (zh) * | 2018-02-01 | 2018-05-18 | 北京同立创辉仪器有限公司 | 全自动核酸提取仪 |
CN109679827A (zh) * | 2019-01-22 | 2019-04-26 | 邹宇彬 | 一种生物医疗移液工作平台 |
CN110514653A (zh) * | 2019-10-28 | 2019-11-29 | 烟台艾德康生物科技有限公司 | 一种化学发光测定仪及其使用方法 |
CN111979119A (zh) * | 2020-09-25 | 2020-11-24 | 北方工业大学 | 一种细胞传代全自动操作装置及其方法 |
WO2022143456A1 (zh) * | 2020-12-29 | 2022-07-07 | 安图实验仪器(郑州)有限公司 | 一种核酸提取及荧光pcr检测系统 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06113814A (ja) * | 1992-10-05 | 1994-04-26 | Hitachi Ltd | 生化学装置 |
JP2014020779A (ja) * | 2012-07-12 | 2014-02-03 | Hitachi High-Technologies Corp | 核酸抽出装置 |
WO2015072941A1 (en) * | 2013-11-15 | 2015-05-21 | Anatolia Tani Ve Biyoteknoloji Urunleri Arastirma Gelistirme Sanayi Ve Ticaret Anonim Sirketi | Laboratory automation system |
WO2017024899A1 (zh) * | 2015-08-11 | 2017-02-16 | 广州康昕瑞基因健康科技有限公司 | 一种试剂工作站 |
CN108048302A (zh) * | 2018-02-01 | 2018-05-18 | 北京同立创辉仪器有限公司 | 全自动核酸提取仪 |
CN208898912U (zh) * | 2018-08-15 | 2019-05-24 | 烟台艾德康生物科技有限公司 | 一种转移液体式核酸提取装置 |
-
2018
- 2018-08-15 CN CN201810928530.0A patent/CN108998444B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06113814A (ja) * | 1992-10-05 | 1994-04-26 | Hitachi Ltd | 生化学装置 |
JP2014020779A (ja) * | 2012-07-12 | 2014-02-03 | Hitachi High-Technologies Corp | 核酸抽出装置 |
WO2015072941A1 (en) * | 2013-11-15 | 2015-05-21 | Anatolia Tani Ve Biyoteknoloji Urunleri Arastirma Gelistirme Sanayi Ve Ticaret Anonim Sirketi | Laboratory automation system |
WO2017024899A1 (zh) * | 2015-08-11 | 2017-02-16 | 广州康昕瑞基因健康科技有限公司 | 一种试剂工作站 |
CN108048302A (zh) * | 2018-02-01 | 2018-05-18 | 北京同立创辉仪器有限公司 | 全自动核酸提取仪 |
CN208898912U (zh) * | 2018-08-15 | 2019-05-24 | 烟台艾德康生物科技有限公司 | 一种转移液体式核酸提取装置 |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108048302A (zh) * | 2018-02-01 | 2018-05-18 | 北京同立创辉仪器有限公司 | 全自动核酸提取仪 |
CN108048302B (zh) * | 2018-02-01 | 2024-03-19 | 北京同立创辉仪器有限公司 | 全自动核酸提取仪 |
CN109679827A (zh) * | 2019-01-22 | 2019-04-26 | 邹宇彬 | 一种生物医疗移液工作平台 |
CN110514653A (zh) * | 2019-10-28 | 2019-11-29 | 烟台艾德康生物科技有限公司 | 一种化学发光测定仪及其使用方法 |
CN111979119A (zh) * | 2020-09-25 | 2020-11-24 | 北方工业大学 | 一种细胞传代全自动操作装置及其方法 |
WO2022143456A1 (zh) * | 2020-12-29 | 2022-07-07 | 安图实验仪器(郑州)有限公司 | 一种核酸提取及荧光pcr检测系统 |
Also Published As
Publication number | Publication date |
---|---|
CN108998444B (zh) | 2023-11-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108998444A (zh) | 一种转移液体式核酸提取装置及方法 | |
CN108949505A (zh) | 一种核酸提取装置及方法 | |
CN208898912U (zh) | 一种转移液体式核酸提取装置 | |
EP2254698B1 (de) | Mobiles gerät für die nukleinsäureisolierung | |
CN103562727A (zh) | 试样处理装置、试样处理方法以及所使用的反应容器 | |
CN104789696B (zh) | 一种用于检测石斑鱼虹彩病毒感染的dna核酸适配体及其筛选方法和应用 | |
DE102008057317A1 (de) | Vorrichtung und Verfahren zur Aufreinigung von Biomolekülen | |
CN108424838A (zh) | 一种快速通过转移磁珠提取dna的装置 | |
DE202014011074U1 (de) | Zentrifuge zum Zentrifugieren einer Reaktions-Gefäßeinheit | |
CN104450498A (zh) | 一种核酸提取仪 | |
DE19512368A1 (de) | System zur Freisetzung und Isolierung von Nukleinsäuren | |
CN109963652B (zh) | 用于提取样品中包含的微生物的提取系统和方法 | |
CN110437970A (zh) | 肠道菌群核酸提取系统 | |
KR101755563B1 (ko) | 핵산 또는 다양한 생물학적 물질을 추출 또는 정제하기 위한 키트 및 자동화장치 | |
CN104673622A (zh) | 核酸扩增反应用筒和核酸扩增反应用筒试剂盒 | |
CN108034565A (zh) | 核酸测序前处理仪 | |
CN105026923A (zh) | 电泳方法以及电泳装置 | |
CN208898865U (zh) | 一种核酸提取装置 | |
CN212025378U (zh) | 一种全自动核酸提取仪 | |
KR101799153B1 (ko) | 시료 내 병원체의 농축 및 검출 겸용 pcr 칩 | |
CN206450486U (zh) | 自动涂片染色机 | |
CN110305773B (zh) | 一种核酸提取装置及提取方法 | |
TWI616525B (zh) | 初代細胞萃取設備及其使用方法 | |
CN211274696U (zh) | 一种自动加塞的免捆扎移液装置 | |
CN113106003A (zh) | 一种微流控芯片及其装置与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Lihai Inventor after: Cao Yiliang Inventor after: Dong Liying Inventor after: Shao Wei Inventor after: Zhang Meng Inventor after: Guo Xinfeng Inventor after: Liu Mengying Inventor before: Zhang Lihai Inventor before: Cao Yiliang Inventor before: Shao Wei Inventor before: Zhang Meng Inventor before: Guo Xinfeng Inventor before: Liu Mengying |
|
GR01 | Patent grant | ||
GR01 | Patent grant |