WO2022143456A1 - 一种核酸提取及荧光pcr检测系统 - Google Patents

一种核酸提取及荧光pcr检测系统 Download PDF

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Publication number
WO2022143456A1
WO2022143456A1 PCT/CN2021/141193 CN2021141193W WO2022143456A1 WO 2022143456 A1 WO2022143456 A1 WO 2022143456A1 CN 2021141193 W CN2021141193 W CN 2021141193W WO 2022143456 A1 WO2022143456 A1 WO 2022143456A1
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Prior art keywords
extraction
nucleic acid
strip
sample
pcr detection
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PCT/CN2021/141193
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English (en)
French (fr)
Inventor
王超
赵鹏
刘耀基
李振坤
潘洋
孙隆
刘聪
Original Assignee
安图实验仪器(郑州)有限公司
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Application filed by 安图实验仪器(郑州)有限公司 filed Critical 安图实验仪器(郑州)有限公司
Priority to EP21914193.4A priority Critical patent/EP4273217A4/en
Priority to US18/265,418 priority patent/US20240027347A1/en
Publication of WO2022143456A1 publication Critical patent/WO2022143456A1/zh

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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/1079—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices with means for piercing stoppers or septums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00108—Test strips, e.g. paper
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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Definitions

  • the invention relates to the technical field of molecular diagnosis, and more particularly, to a nucleic acid extraction and fluorescent PCR detection system.
  • the nucleic acid extraction systems on the market mostly adopt the batch injection method, and at the same time, there are few detection items.
  • the supply of samples, extraction reagent strips, and disposal of consumables are mostly manual methods, which can no longer meet the market demand for projects with a large number of diagnoses.
  • the sample to be tested needs to be tested in advance or manually scanned the barcode, and placed in the test position manually.
  • the test process is complicated, resulting in high labor intensity.
  • the batch injection method will result in that the detection operation of the new sample cannot be carried out in time when the new sample needs to be tested. The flexibility is poor and the detection efficiency is low.
  • the purpose of the present invention is to provide a nucleic acid extraction and fluorescent PCR detection system, which can effectively improve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction and detection system, and make the sample detection process more flexible and convenient.
  • the present invention provides the following technical solutions:
  • a nucleic acid extraction and fluorescent PCR detection system comprising: an extraction strip, an extraction strip loading device for loading the extraction strip with the function of adding the extraction strip online, and a sample rack for loading and unloading samples
  • a loading device an extraction strip transfer gripper for driving the extraction strip to a preset position, a nucleic acid extraction device for purifying the sample to obtain nucleic acid, and a fluorescent PCR detection device for the nucleic acid PCR detection device and control device;
  • the extraction strip loading device, the sample rack loading device, the extraction strip transfer gripper, the nucleic acid extraction device and the PCR detection device are all connected to the control device.
  • the extraction strip loading device comprises: a channel and a pusher device for pushing the extraction strip to move in the channel;
  • One end of the channel is the grasping place of the extraction strip, the pusher device can move laterally, and the pusher device is connected with the control device.
  • a lifting device that can be lifted and lowered for separating the extraction strip is provided at the grabbing position, and the lifting device is connected to the control device.
  • the sample rack loading device includes: a sample rack for loading samples, a loading pusher, an emergency pusher, a scanner, a transfer pusher, and a loading pusher, the transfer pusher is used for transferring the sample to the scanning the scanning position of the instrument, and move the scanned sample to the sampling position, and the loading pusher is used to transport the sampled sample back to the sample recovery area;
  • the loading pusher, the emergency pusher, the scanner, the transfer pusher, and the loading pusher are all connected to the control device.
  • the transfer gripper for the extraction strip includes: an openable and closable gripper device for grasping the extraction strip, a gripper lifting device for driving the lifting movement of the gripper device, and a gripper lifting device for driving the gripper device.
  • a gripper traverse device for lateral movement of the gripper lifting device, the gripper device is arranged on the gripper lifting device, and the gripper lifting device and the gripper traverse device are slidably connected;
  • the gripper device, the gripper lifting device and the gripper traverse device are all connected with the control device.
  • the nucleic acid extraction device comprises: a sealing membrane piercing device for piercing the sealing membrane of the extraction strip, a filling device for adding the sample to be tested to the extraction strip, a reagent A needle, a magnetic bead cleaning device, a high-temperature dissociation device for performing a high-temperature incubation operation on the sample, and a purification liquid magnetic suction device for magnetically purifying the sample to obtain a nucleic acid purification liquid;
  • the reagent needle is used for adding corresponding reagents to the extraction strip or PCR tube and for adding the nucleic acid purification solution of the extraction strip to the PCR tube.
  • the sealing film piercing device comprises: a piercer and a driving component, the driving component is connected with the piercing device, when the piercing device moves to the piercing station, the piercing device will The sealing film of the extraction strip is pierced, and the driving assembly is connected with the control device.
  • the filling device includes: a sample needle for sucking the sample and a sample moving device for driving the sample needle to move in space, the sample needle is connected to the sample moving device, and the sample A mobile device is connected to the control device.
  • the high temperature dissociation device includes an incubation heating block for heating the reaction chamber of the extraction strip and a heating block driving device, and the incubation heating block is connected to the control device.
  • the magnetic bead cleaning device comprises: a washing liquid needle, a washing liquid driving device for driving the washing liquid needle to move in space, and a magnet device for selectively applying a magnetic field to the reaction chamber of the extraction strip and magnet drive means for driving said magnet means to rotate;
  • the magnet driving device is connected with the magnet device, and the lotion needle is connected with the lotion driving device;
  • Both the magnet drive device and the lotion drive device are connected to the control device.
  • it also includes a refrigerated reagent supply device with a refrigerating function for providing different reagents.
  • it also includes a discarding device for recycling the discarded extraction strip, and the discarding device is connected to the control device.
  • the PCR detection device includes a fluorescent PCR detection device for performing fluorescent PCR detection of different items on the sealed PCR tube;
  • the fluorescent PCR detection device is connected with the control device.
  • the fluorescent PCR detection device includes: a casing, a photometric assembly and a temperature control component, the casing is provided with a number of test positions for placing the PCR tube, and each of the test positions is provided with a corresponding test position. the temperature control component, so that each of the test positions can independently control the temperature rise and fall, and the photometric component and the temperature control component are both connected to the control device;
  • the light metering assembly includes a light source assembly for providing a test light source and a driving component for driving the light source assembly to move; the number of the light source assemblies is multiple, and each of the light source assemblies can provide different types of light sources, And each of the light source components can illuminate each of the test positions under the driving of the driving component.
  • the control device can control the extraction strip loading device to automatically load the extraction strip, and control the sample rack loading device to load the sample to be tested, and then control the nucleic acid
  • the extraction device is running, the sample and the reagents required for the reaction can be added to the extraction strip, and then the extraction strip is moved, and the samples and reagents in the extraction strip are subjected to purification operations such as incubation and cleaning to obtain nucleic acid purification solution.
  • the PCR detection device to perform fluorescent PCR detection on the nucleic acid, and the detection result can be fed back to the control device in real time.
  • the position movement of the extraction strip between the devices can be realized by the extraction strip transfer gripper, and by controlling the extraction strip transfer gripper, the spatial movement of the extraction strip can be realized, so that the extraction strip can reach the desired position and perform corresponding operation reactions, etc. .
  • the automatic operation of nucleic acid extraction and detection can be realized, which is beneficial to reduce the intensity of manual operation and improve the detection efficiency of the system.
  • the extraction strip loading device of the device has the function of adding extraction strips online, that is, it is not necessary to immediately interrupt the previous extraction strip pushing and transporting operation every time a new extraction strip is added, which is beneficial to improve the automation degree of the extraction strip loading device. And the continuous conveying effect is beneficial to improve the efficiency of sample detection.
  • the nucleic acid extraction and fluorescent PCR detection system provided by the present invention can effectively improve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction and detection system, and make the sample detection process more flexible and convenient.
  • Fig. 1 is the structural representation of nucleic acid extraction and fluorescent PCR detection system provided by the present invention
  • Fig. 2 is the structural representation of extraction bar
  • Fig. 3 is the structural representation of extraction strip loading device
  • FIG. 4 is a schematic structural diagram of a sample rack loading device
  • Fig. 5 is the structural representation of extraction strip transfer gripper
  • Fig. 6 is the structural representation of the sealing film piercing device
  • FIG. 7 is a schematic structural diagram of a reagent needle
  • FIG. 8 is a schematic structural diagram of a magnetic bead cleaning device
  • Fig. 9 is the structural representation of the waste device
  • Figure 10 is a schematic structural diagram of a PCR tube transfer device
  • Figure 11 is a schematic structural diagram of a PCR tube transfer gripper
  • FIG. 12 is a schematic structural diagram of a fluorescent PCR detection device
  • Figure 13 is a schematic structural diagram of a filling device
  • Fig. 14 is the structural representation of low temperature cracking device
  • 15 is a schematic structural diagram of a high temperature dissociation device
  • Fig. 16 is the structural representation of the purified liquid magnetic suction device
  • 17 is a schematic structural diagram of a consumables supply device
  • Fig. 18 is the structural representation of TIP
  • Figure 19 is a schematic structural diagram of a PCR cover
  • Fig. 20 is the structural representation of PCR tube
  • FIG. 21 is a schematic structural diagram of a refrigerated reagent supply device.
  • 1 extraction strip
  • 101 is hanging ear
  • 102 is 300ul TIP
  • 103 dissociation reagent
  • 104 is logo
  • 105 is washing solution
  • 106 is nucleic acid release solution
  • 107 is magnetic bead
  • 108 is 2ml TIP
  • 109 is reaction chamber
  • 2 is the strip loading device
  • 201 is the first channel
  • 202 is the second channel
  • 203 is the first pusher device
  • 204 is the second pusher device
  • 205 is the transfer device
  • 206 is the loading port
  • 207 is the grabbing 208 is the jacking device
  • 209 is the escape hole
  • 3 is the sample rack loading device
  • 301 is the sample rack
  • 302 is the loading pusher
  • 303 is the emergency pusher
  • 304 is the scanner
  • 305 is the transfer pusher
  • 306 is the loading pusher
  • the pusher, 307 is the sampling place
  • 4 is the extraction strip transfer gripper
  • 401 is the gripper device
  • the core of the present invention is to provide a nucleic acid extraction and fluorescent PCR detection system, which can effectively improve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction and detection system, and make the sample detection process more flexible and convenient.
  • This specific embodiment provides a nucleic acid extraction and fluorescent PCR detection system, including: an extraction strip 1, an extraction strip loading device 2 with the function of adding the extraction strip 1 online and used for loading the extraction strip 1, and an extraction strip loading device 2 for loading and A sample rack loading device 3 for carrying out single or multiple samples, an extraction strip transfer gripper 4 for driving the extraction strip 1 to move to a preset position, a nucleic acid extraction device for purifying the sample to obtain nucleic acid, for PCR detection device and control device for fluorescent PCR detection of nucleic acid; extraction strip loading device 2, sample rack loading device 3, extraction strip transfer gripper 4, nucleic acid extraction device and PCR detection device are all connected to the control device.
  • the structure of the extraction strip 1 is shown in FIG. 2 , which has a plurality of reagent compartments for placing reagents and several empty compartments, wherein the types of reagents placed on the extraction strip 1 include dissociation reagent 103 , washing Liquid 105 , nucleic acid releasing liquid 106 , magnetic beads 107 , etc., the tops of these reagent chambers are provided with sealing membranes. Therefore, when the extraction strip 1 is added, it is necessary to perform a membrane piercing operation in advance.
  • the end of the extraction strip 1 is provided with a reaction chamber 109, and 2ml of TIP108 and 300ul of TIP102 are also placed on the extraction strip 1.
  • the inner holes above the two TIPs can be matched with the same TIP adapter, and both ends of the extraction strip 1 are provided with hangers.
  • the ear 101 and, in addition, the extraction strip 1 are provided with recognizable markings 104 .
  • the structure and size of the extraction strip loading device 2, the sample rack loading device 3, the extraction strip transfer gripper 4, the nucleic acid extraction device, the PCR detection device and the control device can be adjusted in the actual application process according to the actual situation and actual needs. , location, etc.
  • the control device can control the extraction strip loading device 2 to automatically load the extraction strip 1, and control the sample rack loading device 3 to load the sample to be tested, and then , control the operation of the nucleic acid extraction device, and add samples and reagents required for the reaction to the extraction strip 1, and then move the extraction strip 1, and perform purification operations such as incubation and cleaning on the samples and reagents in the extraction strip 1.
  • a nucleic acid purification solution is obtained, and finally, a PCR detection device is used to perform fluorescent PCR detection on the nucleic acid, and the detection result can be fed back to the control device in real time.
  • the position movement of the extraction bar 1 between the devices can be realized by the extraction bar transfer gripper 4, and by controlling the extraction bar transfer gripper 4, the spatial movement of the extraction bar 1 can be realized, so that the extraction bar 1 can reach the desired position. Corresponding operating reactions, etc. In this way, the automatic operation of nucleic acid extraction and detection can be realized, which is beneficial to reduce the intensity of manual operation and improve the detection efficiency of the system.
  • the extraction strip loading device 2 of the device has the function of adding extraction strips 1 online, that is, it is not necessary to immediately interrupt the previous extraction strip 1 pushing and transporting operation every time a new extraction strip 1 is added, which is beneficial to improve the loading of extraction strips.
  • the automation degree and continuous conveying effect of the device 2 are beneficial to improve the sample detection efficiency.
  • the nucleic acid extraction and fluorescent PCR detection system provided by the present invention can effectively improve the problems of poor flexibility and low detection efficiency of the nucleic acid extraction and detection system, and make the sample detection process more flexible and convenient.
  • the extraction strip loading device 2 includes: a channel and a pusher device for pushing the extraction strip 1 to move in the channel; one end of the channel is the grasping place 207 of the extraction strip 1, and the pusher device It can be moved laterally, and the pusher device is connected with the control device.
  • the channel may include a first channel 201 and a second channel 202 arranged in parallel with the first channel 201 to facilitate placing more extraction strips 1 .
  • a first pusher device 203 can be set in the first channel 201
  • a second pusher device 204 can be set in the second channel 202
  • the first pusher device 203 is used to push the extraction strip 1 to move in the first channel 201
  • the second pusher device 204 is used to push the extraction strip 1 to move in the second channel 202
  • a transfer device 205 can be provided to transfer the extraction strip 1 from the first channel 201 to the second channel 202
  • the transfer device 205 can be provided in the first channel 201.
  • the other end of the second channel 202, the first pusher device 203, the second pusher device 204 and the transfer device 205 are all connected with the control device.
  • both the first pusher device 203 and the second pusher device 204 can be moved laterally and up and down, so as to make the placement of the extraction strip 1 more random.
  • the pusher device may also not have a lifting function.
  • a sensor can be set so that each time an extraction strip 1 is added, the sensor can be triggered, so that the pusher device can return to the initial position for the loading operation.
  • the extraction strip loading device 2 can be arranged in front of the system to facilitate the placement of the extraction strip 1 . Wherein, any position of the first channel 201 and the second channel 202 can be used as the loading port 206 of the extraction strip 1 .
  • the left end of the second channel 202 can be provided as the grip 207 of the extraction strip 1
  • the transfer device 205 can be provided at the right end of the first channel 201 and the second channel 202 .
  • the shape, structure and size of the extraction strip 1, the first channel 201, the second channel 202, the first pusher device 203, the second pusher device 204 and the transfer device 205 can be adjusted in the actual application process according to the actual situation and actual needs. , location, etc.
  • the extraction strip loading device 2 loads the extraction strip 1, firstly, the extraction strip 1 is loaded at the loading port 206 of the first channel 201, and then, during the lateral movement of the first pusher device 203, the extraction strip 1 can be pushed in the first
  • the extraction bar 1 reaches the right end position of the first channel 201
  • the extraction bar 1 can be transferred into the second channel 202 by the transfer device 205, and then the extraction bar 1 can be moved by the second pusher device 204. Transferred from the right end of the second channel 202 to the left end, when the extraction strip 1 reaches the grasping position 207 of the second channel 202, the extraction strip transfer gripper 4 can grasp the extraction strip 1, so as to facilitate the extraction of the extraction strip 1. Nucleic acid extraction procedures.
  • the moving, conveying and storing operations of the extraction strip 1 can be realized, and the first pushing handle device 203 and the second pushing handle device 204 of the device can perform lateral movement operations, and can also perform lateral movement operations. Therefore, when the extraction bar 1 is newly added in the first channel 201 or the second channel 202, the first pushing handle device 203 or the second pushing handle device 204 can continue the current horizontal pushing action of the extraction bar 1 without immediately returning to the initial position.
  • the device can realize the online adding operation of the extraction strip 1, and does not need to interrupt the previous pushing and transportation operation immediately every time a new extraction strip 1 is added, which is beneficial to improve the automation degree and use effect of the device.
  • the grabbing part 207 is provided with a liftable lifting device 208 for separating the extraction strip 1 , and the lifting device 208 is connected with the control device.
  • the lifting device 208 is provided at the grasping place 207 to separate the extraction strips 1 that are in close contact with the front and rear, and the extraction strip 1 lifted by the lifting device 208 can be more conveniently and accurately transferred by the extraction strip Hand 4 grabs.
  • the extraction bar 1 can be provided with the first pusher device 203 and the second pusher device after the descent. Avoidance hole 209 through which device 204 passes. In this way, it can be ensured that when the first pushing handle device 203 and the second pushing handle device 204 are lowered, the extraction bar 1 can be smoothly avoided to avoid collision or overturning of the extraction bar 1 .
  • the sample rack loading device 3 includes: a sample rack 301 for loading single or multiple samples, a loading pusher 302, an emergency pusher 303, a scanner 304, a transfer pusher 305, and a loading pusher 306.
  • the transfer pusher 305 is used for When the sample is transferred to the scanning position of the scanner 304 and the scanned sample is moved to the sampling position 307, the pusher 306 is loaded to transport the sampled sample back to the sample recovery area; the pusher 302, the emergency pusher 303, The scanner 304, the transfer pusher 305 and the carry-out pusher 306 are all connected to the control device.
  • the sample rack loading device 3 may be arranged in front of the system to facilitate placement of the sample rack 301 . Wherein, a plurality of sample tubes 5 can be placed on the sample rack 301, and the sample tubes 5 are used for placing the sample liquid.
  • the transfer pusher 305 can transfer the sample rack 301 loaded by the loading pusher 302 or the emergency pusher 303 to the barcode scanning position of the scanner 304.
  • the sample rack 301 can be moved again.
  • the sample tubes 5 on the sample rack 301 are moved to the sampling position 307 in sequence, so that the subsequent filling device 8 can perform the sampling operation on the sample tubes 5.
  • use the push-out handle 306 Push the sample holder 301 to the sample recovery area.
  • the samples can be put into the emergency pusher 303 to perform a queue-cutting detection operation.
  • the sample to be tested can be placed on the sample rack loading device at any time, and there is no need to wait for the previous batch of preset samples to be tested before performing the testing operation, so as to improve the sample testing efficiency and use. flexibility.
  • the shape, structure and size of the sample rack 301 , the sample tube 5 , the loading pusher 302 , the emergency pusher 303 , the scanner 304 , the transfer pusher 305 and the loading pusher 306 can be adjusted in the actual application process according to the actual situation and actual needs. , material, location, etc.
  • the transfer gripper 4 for the extraction strip includes: a gripper device 401 for grasping the extraction strip 1 that can be opened and closed, and a gripper lifting device for driving the lifting and lowering movement of the gripper device 401 402 and a gripper traverse device 403 for driving the gripper lifting device 402 to move laterally, the gripper device 401 is arranged on the gripper lifting device 402, and the gripper lifting device 402 and the gripper traverse device 403 are slidably connected; The device 401 , the gripper lifting device 402 and the gripper traverse device 403 are all connected with the control device.
  • the extraction bar 1 can be effectively grasped, and the extraction bar 1 can be driven to move, so that the extraction bar 1 reaches the desired position for corresponding operations.
  • the extraction strip transfer gripper 4 can be arranged on the sealing film piercing device 7, the filling device 8, the low temperature cracking device 9, the magnetic bead cleaning device 10, the high temperature dissociation device 11, and the purification liquid magnetic suction device 6. , in front of the waste device 18 .
  • the extraction strip transfer gripper 4 grabs the extraction strip 1 from the extraction strip 1 grasping position on the extraction strip loading device 2 , and after successfully grasping the extraction strip 1 , transfers the extraction strip 1 to the front of the sealing film piercing device 7 . , in order to facilitate the sealing film piercing operation. pass
  • the shape, structure, size, position, etc. of the gripper device 401 , the gripper lifting device 402 , and the gripper traverse device 403 may be determined in the actual application process according to the actual situation and actual demand.
  • the nucleic acid extraction device includes: a sealing membrane piercing device 7 for piercing the sealing membrane of the extraction strip 1 , a filling device for adding the sample to be tested to the extraction strip 1
  • the device 8 the reagent needle 12, the magnetic bead cleaning device 10, the high temperature dissociation device 11 for performing the high temperature incubation operation on the sample, and the purification liquid magnetic suction device 6 for magnetically purifying the sample to obtain the nucleic acid purification liquid
  • the reagent needle 12 is used for adding corresponding reagents into the extraction strip 1 or the PCR tube 1704 and adding the nucleic acid purification solution of the extraction strip 1 into the PCR tube 1704 .
  • the reagent needle 12 can also be used to obtain the PCR cover 1705 , snap the PCR cover 1705 to the PCR tube 1704 and transfer the snapped PCR tube 1704 .
  • the sealing film piercing device 7 includes: a piercer 701 and a driving component, the driving component is connected with the piercing device 701 to drive the piercing device 701 to move, when the piercing device 701 moves to the piercing station, the piercing The device 701 pierces the sealing film of the extraction strip 1, and the drive assembly is connected with the control device. Therefore, the sealing film on the extraction strip 1 is pierced by the piercer 701, which effectively solves the problem that the sealing film on the top of the extraction strip 1 is punctured before the reagent in the extraction strip 1 is taken.
  • the sealing film piercing device 7 may further include a cleaning device 702, the cleaning device 702 is connected with the control device, and the cleaning device 702 is used for cleaning the piercing device 701 when the piercing device 701 moves to the cleaning station.
  • the cleaning device 702 By using the cleaning device 702 to clean the piercer 701, the piercer 701 can be reused, which effectively reduces the use of consumables and reduces the cost of the user.
  • the sealing film piercing device 7 has a simple structure, is safe and reliable, has little system pollution risk, and is easy to maintain.
  • the sealing film piercing device 7 can be arranged at a position close to the refrigerated reagent supply device 16 to facilitate subsequent addition of refrigerated reagents.
  • the sealing film piercing device 7 may be equipped with a piercing front and rear pusher 703 , which is used in cooperation with the extraction strip transfer gripper 4 to realize the three-dimensional movement of the extraction strip 1 . Therefore, the pusher 703 before and after piercing can push the extraction strip 1 from the extraction strip transfer gripper 4 into the sealing film piercing device 7, and after the piercing operation is completed, the extraction strip 1 can be pushed back to the extraction strip transfer gripper 4. The extraction strip transfer gripper 4 then transfers the extraction strip 1 to the front of the filling device 8 .
  • the sealing film piercing device 7 may be configured with a scanning instrument 704 to identify the mark 104 on the extraction strip 1 .
  • the reagent needle 12 can obtain the TIP1703 from the consumables supply device 17 , and then absorb the relevant reagents for participating in the reaction from the refrigerated reagent supply device 16 , and add the reagents to the extraction strip 1 .
  • the reaction chamber 109 finally, the reagent needle 12 will discard the used TIP 1703 so as to facilitate the next operation.
  • the shape, structure, size, position, etc. of the puncturing device, the cleaning device 702 , the pusher 703 before and after puncturing, and the scanning instrument can be determined in the actual application process according to the actual situation and actual demand.
  • the filling device 8 includes: a sample needle 801 for sucking a sample and a sample moving device 802 for driving the sample needle 801 to move in space, the sample needle 801 is connected to the sample moving device 802, and the sample moving device 802 is connected to the control device connect.
  • the sample moving device 802 may include a sample forward-backward motion robotic arm 8022 for driving the sample needle 801 to move back and forth, and a sample vertical motion robotic arm 8021 for driving the sample needle 801 to move up and down.
  • a first Front and rear pushers 803 the first front and rear pushers are used in conjunction with the extraction strip transfer gripper 4 to realize three-dimensional movement of the extraction strip 1 and ensure that the extraction strip 1 can be moved to the filling position of the filling device 8 .
  • the first front and rear pushers can push the extraction strip 1 from the extraction strip transfer gripper 4 into the filling device 8, and after the filling operation is completed, push the extraction strip 1 back to the extraction strip transfer gripper 4, and then , the extraction strip transfer gripper 4 can transfer the extraction strip 1 to the front of the cryogenic cracking device 9 .
  • the bottom of the sample needle 801 is vertically provided with a TIP adapter, and the TIP adapter is used to obtain 2ml of TIP108 on the extraction strip 1 .
  • the sample needle 801 can obtain 2ml of TIP108 from the extraction strip 1, and the sample needle 801 moves forward to above the sampling position 307 of the sample rack loading device 3, and then , the sample moving device 802 drives the sample needle 801 to move to extract the sample to be tested, and adds the sample to the reaction chamber 109 of the extraction strip 1.
  • the sample needle 801 extracts the required reagent from the reagent hole on the extraction strip 1
  • the reagents are added into the reaction chamber 109, and the required reagents include: magnetic beads 107 and nucleic acid releasing liquid 106 required for the reaction, and then the liquid in the reaction chamber 109 is sucked and mixed to complete the filling operation. Finally, the sample needle 801 withdraws 2ml of TIP108 onto the extraction strip 1.
  • the shape, structure, size, position, etc. of the sample needle 801 and the sample moving device 802 may be determined in the actual application process according to the actual situation and actual demand.
  • the nucleic acid extraction device may also include a low-temperature lysis device 9, because the sample liquid generally needs to undergo a low-temperature incubation operation in the low-temperature lysis device 9, and the low-temperature incubation operation is conducive to the binding of nucleic acid and magnetic beads 107, and then through the magnetic beads.
  • the cleaning device 10 performs a cleaning operation on the magnetic beads 107 to remove other substances other than the nucleic acid and the magnetic bead 107 combination, and then undergoes a high-temperature incubation operation by the high-temperature dissociation device 11.
  • the high-temperature incubation operation is conducive to nucleic acid and Magnetic beads 107 are separated.
  • the low temperature incubation operation of the low temperature lysing device 9 is not required.
  • the front of the magnetic bead cleaning device 10 does not need to be transferred to the cryogenic cracking device 9 .
  • the high temperature dissociation device 11 includes an incubation heating block 902 for heating the reaction chamber 109 of the extraction strip 1, and the incubation heating block 902 is connected to the control device.
  • a heating block driving device connected to the incubation heating block 902 can be provided, and the heating block driving device is connected to the control device, and the heating block driving device is used to drive the incubation heating block 902 to move in space, so that the heating block near or far from the reaction chamber 109 .
  • the structures of the low-temperature cracking device 9 and the high-temperature dissociation device 11 are similar, mainly because the heating temperature of the incubation heating block 902 is different. The heating temperature is higher.
  • the heating block driving device can be controlled to operate to drive the incubation heating block 902 to approach the reaction chamber 109, and the reaction chamber 109 is heated.
  • the incubation heating block 902 can be separated from the reaction chamber 109.
  • the nucleic acid will be released from the sample solution under the action of the nucleic acid releasing solution 106 , and the nucleic acid will be attached to the magnetic beads 107 .
  • cryogenic cracking device 9 may be configured with a second front and rear pusher 901 , which is used in conjunction with the extraction strip transfer gripper 4 to realize the three-dimensional movement of the extraction strip 1 . Therefore, the second front and rear pushers 901 can push the extraction strip 1 from the extraction strip transfer grip 4 into the cryogenic cracking device 9, and after the low temperature incubation operation is completed, push the extraction strip 1 back to the extraction strip transfer grip 4 , the extraction strip transfer gripper 4 then transfers the extraction strip 1 to the front of the magnetic bead cleaning device 10 .
  • the magnetic bead cleaning device 10 includes: a washing liquid needle 1001 , a washing liquid driving device 1002 for driving the washing liquid needle 1001 to move in space, and a magnet device for selectively applying a magnetic field to the reaction chamber 109 of the extraction strip 1 1003 and a magnet drive device for driving the rotation of the magnet device 1003; the magnet drive device is connected with the magnet device 1003, the lotion needle 1001 is connected with the lotion drive device 1002; the magnet drive device and the lotion drive device 1002 are both connected with the control device.
  • the magnetic bead cleaning device 10 may be configured with a fourth front and rear pusher 1004, which is used in conjunction with the extraction strip transfer gripper 4 to realize the three-dimensional movement of the extraction strip 1. Therefore, the fourth front and rear pushers 1004 can push the extraction strip 1 from the extraction strip transfer gripper 4 into the magnetic bead cleaning device 10 to perform a magnetic suction operation.
  • the lotion needle 1001 is provided with a TIP adapter in the vertical direction, and the TIP adapter is used to obtain 2ml of TIP108 on the extraction strip 1 .
  • 2ml of TIP108 on the extraction strip 1 can be aligned with the lotion needle 1001, and the lotion needle 1001 can be moved downward to obtain 2ml of TIP108; then, the extraction strip 1 can be moved to the magnetic suction position, by rotating
  • the magnet device 1003 makes one of the first magnet or the second magnet turn to the magnetic attraction position, so that the nucleic acid-attached magnetic beads 107 in the reaction chamber 109 of the extraction strip 1 are accumulated in the reaction chamber 109 under the action of magnetic force.
  • the reaction chamber 109 of the extraction strip 1 On the side wall, during magnetic attraction, the reaction chamber 109 of the extraction strip 1 is located under the washing liquid needle 1001, the washing liquid needle 1001 downwardly draws away the liquid in the reaction chamber 109, and the magnetic beads 107 remain in the reaction chamber under the action of magnetic force 109 inner wall, and then, rotate the magnet to make the magnet leave the magnetic suction position, and need to avoid the movement space of the extraction strip 1; after that, push the extraction strip 1, so that the empty space on the extraction strip 1 is aligned with the lotion needle 1001, and the lotion The needle 1001 spit out the liquid sucked in 2ml of TIP108; push the extraction strip 1, so that the reagent chamber on which the lotion 105 is placed on the extraction strip 1 is aligned with the lotion needle 1001, and the lotion needle 1001 sucks the lotion 105; push the extraction strip 1, The reaction chamber 109 on the extraction strip 1 is aligned with the washing liquid needle 1001, and the washing liquid needle 1001 adds the washing liquid 105 in 2m
  • the fourth front and rear pushers 1004 push the extraction strip 1 back to the extraction strip transfer gripper 4 , and the extraction strip transfer gripper 4 then transfers the extraction strip 1 to the front of the high temperature dissociation device 11 .
  • the high temperature dissociation device 11 may be configured with a third front and rear pusher 1101 , which is used in conjunction with the extraction strip transfer gripper 4 to realize three-dimensional movement of the extraction strip 1 . Therefore, the third front and rear pusher 1101 can push the extraction strip 1 from the extraction strip transfer gripper 4 into the high temperature dissociation device 11 for high temperature incubation.
  • the nucleic acid attached to the magnetic beads 107 can be separated from the magnetic beads 107.
  • the extraction strip 1 is pushed back to the extraction strip transfer gripper 4, and the extraction strip transfer gripper 4 then removes the extraction strip 1 Transfer to the front of the purified liquid magnetic suction device 6.
  • the purification liquid magnetic suction device 6 can be arranged within the movement range of the reagent needle 12 to facilitate the reagent needle 12 to absorb the purification liquid in the reaction chamber 109 of the extraction strip 1 .
  • the purification liquid magnetic suction device 6 may be equipped with a pusher 601 and a magnet 602 before and after purification.
  • the pusher 601 before and after the purification is used in conjunction with the extraction strip transfer gripper 4 to realize the three-dimensional space movement of the extraction strip 1 . Therefore, the pusher 601 before and after purification can push the extraction strip 1 from the extraction strip transfer gripper 4 into the purification liquid magnetic suction device 6 for purification operation, and after the purification operation is completed, push the extraction strip 1 back to the extraction strip transfer gripper 4.
  • the extraction strip transfer gripper 4 then transfers the extraction strip 1 to the front of the disposal device 18 .
  • the magnet 602 is used to apply a magnetic force to the reaction chamber 109 of the extraction strip 1, so that the magnetic beads 107 in the reaction chamber 109 are accumulated on the side wall of the reaction chamber 109 under the action of the magnetic force, so that the nucleic acid remains in the liquid, thereby obtaining the required detection requirements. nucleic acid purification solution.
  • a refrigerated reagent supply device 16 with a refrigeration function for providing different reagents is also included.
  • the refrigerated reagent supply device 16 is equipped with a refrigeration function to better maintain the reagents that need to be refrigerated, and a liquid suction hole 1601 can be provided on the top of the refrigerated reagent supply device 16, and the liquid suction hole 1601 is connected to the reagent.
  • the sizes of the needles 12 are matched to facilitate the reagent needle 12 to absorb the reagent.
  • the system may further include a consumables supply device 17 for providing TIP1703, PCR tubes 1704 and PCR covers 1705, and the consumables supplying device 17 is provided with a TIP consumables rack 1701 for placing the TIP1703 and a TIP consumables rack 1701 for placing the PCR tubes 1704 and 1705.
  • PCR consumable rack 1702 for PCR cover 1705 is provided with a TIP consumables rack 1701 for placing the TIP1703 and a TIP consumables rack 1701 for placing the PCR tubes 1704 and 1705.
  • the TIP 1703 and the PCR cover 1705 are provided with inner holes above, and the inner holes are matched with the size of the reagent needle 12 , so that the reagent needle 12 can take the TIP 1703 and the PCR cover 1705 easily.
  • the inner holes above the TIP 1703 and the PCR cover 1705 can be matched with the TIP adapter holes of the reagent needle 12, so that the reagent needle 12 can take the TIP 1703 and the PCR cover 1705.
  • the reagent needle 12 can use the structure of horizontal, front and rear and vertical three-dimensional movement mechanical arms to achieve spatial movement, and the reagent needle 12 can be provided with a TIP adapter in the vertical direction, and the TIP adapter is used to obtain TIP1703 or PCR cover 1705,
  • the movement range of the reagent needle 12 covers: the magnetic suction device 6 for the purified liquid, the sealing film piercing device 7, the consumables supply device 17, the refrigerated reagent supply device 16, etc. After the reagent needle 12 obtains the TIP1703, it can be placed on the refrigerated reagent supply device 16.
  • the reagent needle 12 can obtain the TIP 1703 from the consumable supply device 17, suck the nucleic acid purification solution from the reaction chamber 109 of the extraction strip 1, and then add the nucleic acid purification solution to the PCR tube 1704 on the consumable supply device 17. Obsolete TIP1703. After the reagent needle 12 obtains the TIP 1703 from the consumable supply device 17, it absorbs the reagent required for the reaction from the refrigerated reagent supply device 16, adds the reagent to the same PCR tube 1704, and discards the TIP 1703 from the TIP disposal port.
  • the reagent needle 12 obtains the PCR cover 1705 from the consumables supply device 17, and snaps the PCR cover 1705 onto the PCR tube 1704 to which the nucleic acid purification solution and reagents have been added. Finally, the reagent needle 12 transfers the sealed PCR tube.
  • the reagent needle 12 may include a needle device 1201 and a needle driving device for driving the needle device 1201 to move in space, and both the needle device 1201 and the needle driving device are connected to the control device.
  • the needle driving device may include a needle horizontal motion mechanical arm 12021 for driving the needle device 1201 to move horizontally, a needle back-and-forth motion mechanical arm 12022 for driving the needle device 1201 to move back and forth, and a needle vertical motion machine for driving the needle device 1201 to move vertically. Arm 12023 to achieve three-dimensional movement of needle device 1201.
  • a waste device 18 for recycling waste extraction strips 1 is further included, and the waste device 18 is connected to the control device.
  • the waste device 18 may include: a waste liquid needle 1801 for sucking the waste liquid of the extraction strip 1 , an openable and closable gripper 1802 for grasping the discarded extraction strip 1 , and for driving the opening and closing gripper
  • the opening and closing drive device for opening or closing the hand 1802 and the elevating drive device for driving the waste liquid needle 1801 to move up and down are connected to the control device.
  • the disposal device 18 may be configured with a fifth front and rear pusher 1803 .
  • the fifth front and rear pusher 1803 is used in conjunction with the extraction strip transfer gripper 4 to realize the three-dimensional movement of the extraction strip 1 . Therefore, the fifth front and rear pusher 1803 can push the extraction strip 1 from the extraction strip transfer gripper 4 into the waste device 18, and then, the reaction chamber 109 and the reagent chambers on the extraction strip 1 are sequentially pushed below the waste liquid needle 1801, The waste needle can be moved in the vertical direction to remove the remaining liquid from the reaction chamber 109 and each reagent chamber. After the liquid pumping operation is completed, the fifth front and rear pusher 1803 pushes the extraction strip 1 to the waste place of the opening and closing gripper 1802. Finally, the opening and closing gripper 1802 is opened to discard the extraction strip 1 there.
  • the PCR detection device includes a fluorescent PCR detection device 13 for performing fluorescent PCR detection of different items on the sealed PCR tube 1704, and the fluorescent PCR detection device 13 is connected to the control device.
  • the PCR detection device may further include a PCR tube transfer device 14 and a PCR tube transfer gripper 15, and both the PCR tube transfer device 14 and the PCR tube transfer gripper 15 are connected to the control device.
  • the PCR tube transfer device 14 is used to transfer the sealed PCR tube 1704 to the grasping position of the PCR tube transfer gripper 15, and the PCR tube transfer gripper 15 is used to transfer the PCR tube 1704 from the PCR tube transfer device 14 to the fluorescent tube PCR detection device 13 .
  • the reagent needle 12 can transfer the sealed PCR tube to the PCR tube transfer device 14 .
  • the PCR tube transfer device 14 is configured with a horizontally movable transfer block 1402, the PCR tube 1704 can be placed in the PCR tube 1704 placement hole 1401 above the transfer block 1402, and then the PCR tube 1704 can be transferred to the grip of the PCR tube transfer gripper 15 take a bit.
  • the fluorescent PCR detection device 13 includes: a casing, a photometric assembly 1301 and a temperature control component, the casing is provided with a number of test positions 1303 for placing the PCR tube 1704, and each test position 1303 is correspondingly provided with a temperature control component, In order that each test position 1303 can be independently controlled for temperature rise and fall, the photometric assembly 1301 and the temperature control component are connected to the control device; the photometric assembly 1301 includes a light source assembly for providing a test light source and a light source assembly for driving the light source assembly to move.
  • the number of light source assemblies is multiple, and each light source assembly can provide different types of light sources, and each light source assembly can illuminate each test position 1303 under the driving of the driving member.
  • the fluorescent PCR detection device 13 may further include a pressing cylinder 1302 for compressing the PCR tube 1704, the housing is provided with a magnetic component, and the pressing cylinder 1302 is also provided with a magnet, so that the pressing cylinder 1302 can be adsorbed on the housing, thereby compressing the PCR tube. Since each test position 1303 is correspondingly provided with a temperature control component for controlling the temperature of the PCR tube 1704, each test position 1303 can independently control the temperature rise and fall, thereby realizing simultaneous online detection of different test items.
  • each test position 1303 is equipped with an independent pressure cylinder 1302.
  • the pressure cylinder 1302 has the function of compressing the PCR tube 1704.
  • the pressure cylinder 1302 can be quickly installed and fixed by means of magnetic attraction, and can ensure the cooperation between the PCR tube 1704 and the temperature control component. close to improve the detection effect.
  • the PCR tube transfer gripper 15 can move in three-dimensional space, and is used to grasp the sealed PCR tube 1704 or the pressure cylinder 1302 so as to cooperate with the fluorescent PCR detection device 13 for detection operation.
  • the PCR tube transfer gripper 15 may include a PCR gripper 1501 for grasping the sealed PCR tube 1704 and the press cylinder 1302, a PCR horizontal motion robotic arm 1502 for driving the PCR gripper 1501 to move horizontally, and a PCR horizontal motion robotic arm 1502 for driving the PCR gripper 1501
  • the PCR gripper 1501 can grab the pressure cylinder 1302 on the fluorescent PCR detection device 13, place the pressure cylinder 1302 on the pressure cylinder placement place 1304, grab the PCR tube 1704 from the PCR tube transfer device 14 and place it on the fluorescent PCR detection device
  • the test position 1303 of the pressure cylinder 1302 on the 13 is opened, and then the pressure cylinder 1302 is grabbed from the pressure cylinder placement place 1304 and placed on the PCR tube 1704. After the fluorescent PCR detection is completed, grab the pressure cylinder 1302 and place it at the place where the pressure cylinder is placed.
  • the nucleic acid extraction and fluorescent PCR detection system includes a frame and an extraction strip loading device 2, a sample rack loading device 3, and an extraction strip transfer gripper 4 respectively arranged on the frame. , sealing film piercing device 7, filling device 8, low temperature cracking device 9, magnetic bead cleaning device 10, high temperature dissociation device 11, purified liquid magnetic suction device 6, waste device 18, consumables supply device 17, refrigerated reagent supply device 16. Reagent needle 12, PCR tube transfer device 14, PCR tube transfer gripper 15, fluorescent PCR detection device 13, etc. Integrating each device into one system makes the system highly integrated, and can realize automatic nucleic acid extraction and automatic detection of fluorescent PCR.
  • the system realizes the automatic loading of extraction reagents through the extraction strip loading device 2; realizes the automatic loading and unloading of the samples to be tested through the sample rack loading device 3; through the extraction strip transfer gripper 4, sealing film Puncture device 7, filling device 8, low temperature cracking device 9, magnetic bead cleaning device 10, high temperature dissociation device 11, purified liquid magnetic suction device 6, waste device 18, consumables supply device 17, refrigerated reagent supply device 16 and addition
  • the injection device 8 realizes the automatic extraction operation of nucleic acid; through the PCR tube transfer device 14 , the PCR tube transfer gripper 15 and the fluorescent PCR detection device 13 , the fluorescent PCR automatic detection operation of nucleic acid is realized.
  • the system supports on-machine testing of samples to be tested at any time. It is equipped with multiple independent temperature rise and fall photometric devices, supports simultaneous on-machine testing of multiple items, and realizes the function of random and random testing of samples to be tested. Effectively reduce the waiting time for sample detection.
  • the whole process of nucleic acid extraction and fluorescent PCR detection of this system has no manual participation, and the degree of automation is high, which can effectively avoid manual errors during detection, improve detection efficiency, and reduce labor intensity.
  • orientation or positional relationship indicated by "up and down”, “front and rear”, etc. in this application is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of simplified description and easy understanding, not An indication or implication that the referred device or element must have a particular orientation, be constructed and operate in a particular orientation, is not to be construed as a limitation of the invention.

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Abstract

一种核酸提取及荧光PCR检测系统，包括：提取条、用于载入提取条的提取条载入装置、用于载入和载出样本的样本架载入装置、用于带动提取条移动至预设位置的提取条转移抓手、用于对样本进行提纯以得到核酸的核酸提取装置、用于对核酸进行荧光PCR检测的PCR检测装置以及控制装置；提取条载入装置、样本架载入装置、提取条转移抓手、核酸提取装置以及PCR检测装置均与控制装置连接。其可改善核酸提取检测系统的灵活性较差、检测效率较低的问题，使样本检测过程更为灵活方便。

Description

一种核酸提取及荧光PCR检测系统
本申请要求于2020年12月29日提交中国专利局、申请号为202011601009.X、发明名称为“一种核酸提取及荧光PCR检测系统”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明涉及分子诊断技术领域，更具体地说，涉及一种核酸提取及荧光PCR检测系统。
背景技术
分子诊断灵敏度高、特异性好、及时方便，已广泛应用于临床，某些项目的诊断上对提取系统的自动化程度、灵活性、通量提出了更高要求。
目前，市场上的核酸提取系统多采用批量进样方式，同时检测项目较少，样本供给、提取试剂条供给、耗材废弃等多采用手工方式，已不满足诊断数量较多的项目的市场需求，现有的核酸提取系统，待测样本需提前编测试或手工扫条码，手工放置到测试位，测试流程复杂，导致人工劳动强度较大。而且，批量进样方式会导致在需要对新样本进行检测时，无法及时进行新样本的检测操作，必须等到前一批的样本检测完成后，才可以对新样本进行检测操作，这会导致系统的灵活性较差、检测效率较低。
因此，如何改善核酸提取检测系统的灵活性较差、检测效率较低的问题，是本领域技术人员亟待解决的技术问题。
发明内容
有鉴于此，本发明的目的是提供一种核酸提取及荧光PCR检测系统，可以有效改善核酸提取检测系统的灵活性较差、检测效率较低的问题，使得样本检测过程更为灵活方便。
为了实现上述目的，本发明提供如下技术方案：
一种核酸提取及荧光PCR检测系统，包括：提取条、具有在线添加所 述提取条功能的用于载入所述提取条的提取条载入装置、用于载入和载出样本的样本架载入装置、用于带动所述提取条移动至预设位置的提取条转移抓手、用于对所述样本进行提纯以得到核酸的核酸提取装置、用于对所述核酸进行荧光PCR检测的PCR检测装置以及控制装置；
所述提取条载入装置、所述样本架载入装置、所述提取条转移抓手、所述核酸提取装置以及所述PCR检测装置均与所述控制装置连接。
优选的，所述提取条载入装置包括：通道和用于推动所述提取条在所述通道内移动的推手装置；
所述通道的一端为所述提取条的抓取处，所述推手装置可横向移动，所述推手装置与所述控制装置连接。
优选的，所述抓取处设有可升降的用于分离所述提取条的顶升装置，所述顶升装置与所述控制装置连接。
优选的，所述样本架载入装置包括：用于装载样本的样本架、载入推手、急诊推手、扫描仪、转移推手以及载出推手，所述转移推手用于将样本转移至所述扫描仪的扫描处、并将扫描后的样本移动至抽样处，所述载出推手用于将抽样后的样本运回样本回收区；
所述载入推手、所述急诊推手、所述扫描仪、所述转移推手以及所述载出推手均与所述控制装置连接。
优选的，所述提取条转移抓手包括：可开合的用于抓取所述提取条的抓手装置、用于带动所述抓手装置升降运动的抓手升降装置以及用于驱动所述抓手升降装置横向移动的抓手横移装置，所述抓手装置设于所述抓手升降装置上，所述抓手升降装置和所述抓手横移装置滑动连接；
所述抓手装置、所述抓手升降装置以及所述抓手横移装置均与所述控制装置连接。
优选的，所述核酸提取装置包括：用于将所述提取条的封膜刺破的封膜刺破装置、用于将待测的所述样本添加至所述提取条的加注装置、试剂针、磁珠清洗装置、用于对所述样本进行高温温育操作的高温解离装置以及用于对所述样本进行磁吸提纯以得到核酸提纯液的提纯液磁吸装置；
所述试剂针用于将对应试剂添加至所述提取条或PCR管内和用于将 所述提取条的核酸提纯液添加至所述PCR管内。
优选的，所述封膜刺破装置包括：刺破器和驱动组件，所述驱动组件与所述刺破器连接，当所述刺破器移动到刺破工位，所述刺破器将所述提取条的封膜刺破，所述驱动组件与所述控制装置连接。
优选的，所述加注装置包括：用于吸取所述样本的样本针和用于驱动所述样本针进行空间移动的样本移动装置，所述样本针与所述样本移动装置连接，所述样本移动装置与所述控制装置连接。
优选的，所述高温解离装置包括用于对所述提取条的反应仓加热的温育加热块和加热块驱动装置，所述温育加热块与所述控制装置连接。
优选的，所述磁珠清洗装置包括：洗液针、用于驱动所述洗液针进行空间移动的洗液驱动装置、用于选择性的对所述提取条的反应仓施加磁场的磁体装置以及用于驱动所述磁体装置转动的磁体驱动装置；
所述磁体驱动装置和所述磁体装置连接，所述洗液针与所述洗液驱动装置连接；
所述磁体驱动装置和所述洗液驱动装置均与所述控制装置连接。
优选的，还包括具有制冷功能的、用于提供不同试剂的冷藏试剂供给装置。
优选的，还包括用于回收废弃的所述提取条的废弃装置，所述废弃装置与所述控制装置连接。
优选的，所述PCR检测装置包括用于对密封后的所述PCR管进行不同项目的荧光PCR检测的荧光PCR检测装置；
所述荧光PCR检测装置与所述控制装置连接。
优选的，所述荧光PCR检测装置包括：壳体、测光组件以及温控部件，所述壳体上设有用于放置所述PCR管的若干测试位，各所述测试位均对应设有所述温控部件，以使各所述测试位相互独立的进行温度升降控制，所述测光组件和所述温控部件均与所述控制装置连接；
所述测光组件包括用于提供测试光源的光源组件以及用于带动所述光源组件运动的驱动部件；所述光源组件的个数为多个，各所述光源组件可提供的光源类型不同，且各所述光源组件可在所述驱动部件的带动下照射 各所述测试位。
在使用本发明所提供的核酸提取及荧光PCR检测系统时，首先，控制装置可以控制提取条载入装置自动载入提取条，并控制样本架载入装置载入待测样本，而后，控制核酸提取装置运行，可以将样本和反应所需试剂等添加至提取条内，再通过移动提取条，并对提取条内的样本和试剂进行温育、清洗等提纯操作，以得到核酸提纯液，最后，再利用PCR检测装置对核酸进行荧光PCR检测，检测结果可实时反馈至控制装置。其中，提取条在各装置之间的位置移动可以通过提取条转移抓手实现，通过控制提取条转移抓手，可实现提取条的空间移动，使提取条到达所需位置进行相应的操作反应等。这样可以实现核酸提取检测的自动化操作，有利于降低人工操作强度，提高系统的检测效率。
由于本装置的提取条载入装置具有在线添加提取条的功能，也即无需每次添加新的提取条时，立即中断先前的提取条推动运输操作，有利于提高提取条载入装置的自动化程度和连续输送效果，有利于提高样本检测效率。
综上所述，本发明所提供的核酸提取及荧光PCR检测系统，可以有效改善核酸提取检测系统的灵活性较差、检测效率较低的问题，使得样本检测过程更为灵活方便。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据提供的附图获得其他的附图。
图1为本发明所提供的核酸提取及荧光PCR检测系统的结构示意图；
图2为提取条的结构示意图；
图3为提取条载入装置的结构示意图；
图4为样本架载入装置的结构示意图；
图5为提取条转移抓手的结构示意图；
图6为封膜刺破装置的结构示意图；
图7为试剂针的结构示意图；
图8为磁珠清洗装置的结构示意图；
图9为废弃装置的结构示意图；
图10为PCR管传输装置的结构示意图；
图11为PCR管转移抓手的结构示意图；
图12为荧光PCR检测装置的结构示意图；
图13为加注装置的结构示意图；
图14为低温裂解装置的结构示意图；
图15为高温解离装置的结构示意图；
图16为提纯液磁吸装置的结构示意图；
图17为耗材供给装置的结构示意图；
图18为TIP的结构示意图；
图19为PCR盖的结构示意图；
图20为PCR管的结构示意图；
图21为冷藏试剂供给装置的结构示意图。
图1-图21中：
1为提取条、101为挂耳、102为300ul TIP、103为解离试剂、104为标识、105为洗液、106为核酸释放液、107为磁珠、108为2ml TIP、109为反应仓、2为提取条载入装置、201为第一通道、202为第二通道、203为第一推手装置、204为第二推手装置、205为转移装置、206为载入口、207为抓取处、208为顶升装置、209为避让孔、3为样本架载入装置、301为样本架、302为载入推手、303为急诊推手、304为扫描仪、305为转移推手、306为载出推手、307为抽样处、4为提取条转移抓手、401为抓手装置、402为抓手升降装置、403为抓手横移装置、5为样本管、6为提纯液磁吸装置、601为提纯前后推手、602为磁铁、7为封膜刺破装置、701为刺破器、702为清洗装置、703为刺破前后推手、704为扫描仪器、8为加注装置、801为样本针、802为样本移动装置、8021为样本垂直运动机械臂、8022为样本前后运动机械臂、803为第一前后推手、9为低温裂解 装置、901为第二前后推手、902为温育加热块、10为磁珠清洗装置、1001为洗液针、1002为洗液驱动装置、1003为磁体装置、1004为第四前后推手、11为高温解离装置、1101为第三前后推手、12为试剂针、1201为针装置、1202为针驱动装置、12021为针水平运动机械臂、12022为针前后运动机械臂、12023为针垂直运动机械臂、13为荧光PCR检测装置、1301为测光组件、1302为压筒、1303为测试位、1304为压筒放置处、1305为PCR管废弃口、14为PCR管传输装置、1401为PCR管放置孔、1402为传输块、15为PCR管转移抓手、1501为PCR抓手、1502为PCR水平运动机械臂、1503为PCR前后运动机械臂、1504为PCR垂直运动机械臂、16为冷藏试剂供给装置、1601为吸液孔、17为耗材供给装置、1701为TIP耗材架、1702为PCR耗材架、1703为TIP、1704为PCR管、1705为PCR盖、18为废弃装置、1801为废液针、1802为开合抓手、1803为第五前后推手。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
本发明的核心是提供一种核酸提取及荧光PCR检测系统，可以有效改善核酸提取检测系统的灵活性较差、检测效率较低的问题，使得样本检测过程更为灵活方便。
请参考图1至图21。
本具体实施例提供了一种核酸提取及荧光PCR检测系统，包括：提取条1、具有在线添加提取条1功能的用于载入提取条1的提取条载入装置2、用于载入和载出单个或多个样本的样本架载入装置3、用于带动提取条1移动至预设位置的提取条转移抓手4、用于对样本进行提纯以得到核酸的核酸提取装置、用于对核酸进行荧光PCR检测的PCR检测装置以及控制 装置；提取条载入装置2、样本架载入装置3、提取条转移抓手4、核酸提取装置以及PCR检测装置均与控制装置连接。
需要说明的是，提取条1的结构如图2所示，其具有多个用于放置试剂的试剂仓和几个空仓，其中，提取条1上放置的试剂种类包括有解离试剂103、洗液105、核酸释放液106、磁珠107等，这些试剂仓的顶部设有封膜，因此，在对提取条1进行添加操作时，需要提前对其进行刺膜操作。提取条1的端部设有反应仓109，提取条1上还放置有2ml TIP108和300ul TIP102，两款TIP上方的内孔可以匹配同一款TIP适配器，而且，提取条1的两端设置有挂耳101，另外，提取条1上设有可识别的标识104。
可以在实际运用过程中，根据实际情况和实际需求，对提取条载入装置2、样本架载入装置3、提取条转移抓手4、核酸提取装置、PCR检测装置以及控制装置的结构、尺寸、位置等进行确定。
在使用本发明所提供的核酸提取及荧光PCR检测系统时，首先，控制装置可以控制提取条载入装置2自动载入提取条1，并控制样本架载入装置3载入待测样本，而后，控制核酸提取装置运行，可以将样本和反应所需试剂等添加至提取条1内，再通过移动提取条1，并对提取条1内的样本和试剂进行温育、清洗等提纯操作，以得到核酸提纯液，最后，再利用PCR检测装置对核酸进行荧光PCR检测，检测结果可实时反馈至控制装置。其中，提取条1在各装置之间的位置移动可以通过提取条转移抓手4实现，通过控制提取条转移抓手4，可实现提取条1的空间移动，使提取条1到达所需位置进行相应的操作反应等。这样可以实现核酸提取检测的自动化操作，有利于降低人工操作强度，提高系统的检测效率。
由于本装置的提取条载入装置2具有在线添加提取条1的功能，也即无需每次添加新的提取条1时，立即中断先前的提取条1推动运输操作，有利于提高提取条载入装置2的自动化程度和连续输送效果有利于提高样本检测效率。
综上所述，本发明所提供的核酸提取及荧光PCR检测系统，可以有效改善核酸提取检测系统的灵活性较差、检测效率较低的问题，使得样本检测过程更为灵活方便。
在上述实施例的基础上，优选的，提取条载入装置2包括：通道和用于推动提取条1在通道内移动的推手装置；通道的一端为提取条1的抓取处207，推手装置可横向移动，推手装置与控制装置连接。
优选的，通道可以包括第一通道201和与第一通道201平行贴合设置的第二通道202，以便于放置更多的提取条1。可以在第一通道201内设置第一推手装置203，在第二通道202内设置第二推手装置204，第一推手装置203用于推动提取条1在第一通道201内移动，第二推手装置204用于推动提取条1在第二通道202内移动，并且，可以设置转移装置205，以将提取条1从第一通道201转移至第二通道202，转移装置205可以设置在第一通道201和第二通道202的另一端，第一推手装置203、第二推手装置204以及转移装置205均与控制装置连接。
优选的，第一推手装置203和第二推手装置204均可横向移动和升降移动，以便于使得提取条1的放置更为随机化。当然，推手装置也可以不具备升降功能，例如可以通过设置传感器，使得每次新增提取条1时，即可触发传感器，使得推手装置回到初始位置进行载入操作。可以将提取条载入装置2布置在本系统的前方，以便于放置提取条1。其中，第一通道201和第二通道202的任意位置均可作为提取条1的载入口206。
例如，可以将第二通道202的左端设置为提取条1的抓取处207，并将转移装置205设置在第一通道201和第二通道202的右端。
可以在实际运用过程中，根据实际情况和实际需求，对提取条1、第一通道201、第二通道202、第一推手装置203、第二推手装置204以及转移装置205的形状、结构、尺寸、位置等进行确定。
在提取条载入装置2载入提取条1时，首先，在第一通道201的载入口206加载提取条1，而后，第一推手装置203横向移动过程中，可推动提取条1在第一通道201内移动，当提取条1到达第一通道201的右端位置时，通过转移装置205可以将提取条1转移至第二通道202内，而后，再通过第二推手装置204将提取条1由第二通道202的右端传输至左端，当提取条1到达第二通道202的抓取处207后，提取条转移抓手4可以对提取条1进行抓取操作，以便于对提取条1进行核酸提取操作。
在第一通道201和第二通道202内可以实现提取条1的移动输送和存储操作，并且，本装置的第一推手装置203和第二推手装置204除了可以进行横向移动操作以外，还可进行升降移动，因此，当第一通道201或第二通道202内新添了提取条1时，第一推手装置203或第二推手装置204可以继续当前的横向推动提取条1的动作，无需立即回到初始位。
当添加提取条1的操作结束后，再控制第一推手装置203或第二推手装置204先下降，以避免碰撞到提取条1，而后将第一推手装置203或第二推手装置204移动至初始位，当需要重新推动新添的提取条1时，再控制第一推手装置203或第二推手装置204上升、并重新推动新添的提取条1移动。因此，本装置可实现提取条1的在线添加操作，也无需每次添加新的提取条1时，立即中断先前的推动运输操作，有利于提高装置的自动化程度和使用效果。
优选的，抓取处207设有可升降的用于分离提取条1的顶升装置208，顶升装置208与控制装置连接。
需要说明的是，在抓取处207设置顶升装置208，是为了将前后紧贴的提取条1进行分离，被顶升装置208顶升的提取条1可以更方便准确的被提取条转移抓手4抓取。
需要说明的是，如果第一推手装置203和第二推手装置204既可横向移动，又可升降运动，则可以在提取条1上设置用于使下降后的第一推手装置203和第二推手装置204通过的避让孔209。这样可确保当第一推手装置203和第二推手装置204下降后能够顺利避开提取条1，以避免碰撞或打翻提取条1。
优选的，样本架载入装置3包括：用于装载单个或多个样本的样本架301、载入推手302、急诊推手303、扫描仪304、转移推手305以及载出推手306，转移推手305用于将样本转移至扫描仪304的扫描处、并将扫描后的样本移动至抽样处307，载出推手306用于将抽样后的样本运回样本回收区；载入推手302、急诊推手303、扫描仪304、转移推手305以及载出推手306均与控制装置连接。可以将样本架载入装置3布置在本系统的前方，以便于放置样本架301。其中，样本架301上可以放置有多个样 本管5，样本管5用于放置样本液。
需要说明的是，转移推手305可以将载入推手302或急诊推手303载入的样本架301转移到扫描仪304的条码扫描处，当样本管5完成了扫描操作后，再移动样本架301，使样本架301上的样本管5依次移动至抽样处307，以便于后续加注装置8对样本管5进行抽样操作后，之后，再移动样本架301至返回位，最后，利用载出推手306将样本架301推动至样本回收区。当某些样本需要进行紧急检测时，可以将该样本放入急诊推手303，进行插队检测操作。当有新的待测样本需要检测时，可随时将待测样本放到样本架载入装置上，无需等待上一批预设样本全部检测完成后才进行检测操作，以提高样本检测效率和使用灵活性。
可以在实际运用过程中，根据实际情况和实际需求，对样本架301、样本管5、载入推手302、急诊推手303、扫描仪304、转移推手305以及载出推手306的形状、结构、尺寸、材质、位置等进行确定。
在上述实施例的基础上，优选的，提取条转移抓手4包括：可开合的用于抓取提取条1的抓手装置401、用于带动抓手装置401升降运动的抓手升降装置402以及用于驱动抓手升降装置402横向移动的抓手横移装置403，抓手装置401设于抓手升降装置402上，抓手升降装置402和抓手横移装置403滑动连接；抓手装置401、抓手升降装置402以及抓手横移装置403均与控制装置连接。因此，通过控制抓手装置401的开合以及抓手装置401的空间运动，可有效抓取提取条1，带动提取条1移动，使提取条1到达所需位置进行相应操作。
需要说明的是，可以将提取条转移抓手4布置在封膜刺破装置7、加注装置8、低温裂解装置9、磁珠清洗装置10、高温解离装置11、提纯液磁吸装置6、废弃装置18的前方。提取条转移抓手4从提取条载入装置2上的提取条1抓取位抓取提取条1，成功抓取提取条1后，再将提取条1转移至封膜刺破装置7的前方，以便于进行封膜刺破操作。通过
可以在实际运用过程中，根据实际情况和实际需求，对抓手装置401、抓手升降装置402以及抓手横移装置403的形状、结构、尺寸、位置等进行确定。
在上述实施例的基础上，优选的，核酸提取装置包括：用于将提取条1的封膜刺破的封膜刺破装置7、用于将待测的样本添加至提取条1的加注装置8、试剂针12、磁珠清洗装置10、用于对样本进行高温温育操作的高温解离装置11以及用于对样本进行磁吸提纯以得到核酸提纯液的提纯液磁吸装置6；试剂针12用于将对应试剂添加至提取条1或PCR管1704内和将提取条1的核酸提纯液添加至PCR管1704内。此外，试剂针12也可以用于获取PCR盖1705、并将PCR盖1705扣合至PCR管1704上以及用于转移扣合后的PCR管1704。
优选的，封膜刺破装置7包括：刺破器701和驱动组件，驱动组件与刺破器701连接，以驱动刺破器701移动，当刺破器701移动到刺破工位，刺破器701将提取条1的封膜刺破，驱动组件与控制装置连接。因此，通过刺破器701刺破提取条1上的封膜，有效解决了取用提取条1内试剂前，提取条1顶部的封膜刺破的问题。
此外，封膜刺破装置7还可以包括清洗装置702，清洗装置702与控制装置连接，清洗装置702用于当刺破器701移动到清洗工位，对刺破器701进行清洗。通过利用清洗装置702将刺破器701清洗干净，使得刺破器701可以重复使用，有效减少了耗材的使用，降低了用户的成本。并且该封膜刺破装置7结构简单，安全可靠，系统污染风险小，同时便于维护。
需要说明的是，可以将封膜刺破装置7布置在靠近冷藏试剂供给装置16的位置，以便于后续添加冷藏试剂。封膜刺破装置7可以配置有刺破前后推手703，该刺破前后推手703用于与提取条转移抓手4配合使用，以实现提取条1的三维空间运动。因此，刺破前后推手703可以从提取条转移抓手4上将提取条1推到封膜刺破装置7内，待刺破操作完成后，再将提取条1推回至提取条转移抓手4，提取条转移抓手4再将提取条1转移到加注装置8的前方。
其中，封膜刺破装置7可以配置有扫描仪器704，以识别提取条1上的标识104。当提取条1的封膜被刺破后，试剂针12可以从耗材供给装置17获取TIP1703，再从冷藏试剂供给装置16上吸取用于参与反应的相关试剂，并将试剂添加到提取条1的反应仓109内，最后，试剂针12会废弃已 使用的TIP1703，以便于进行下次操作。
可以在实际运用过程中，根据实际情况和实际需求，对刺破装置、清洗装置702、刺破前后推手703以及扫描仪器的形状、结构、尺寸、位置等进行确定。
优选的，加注装置8包括：用于吸取样本的样本针801和用于驱动样本针801进行空间移动的样本移动装置802，样本针801与样本移动装置802连接，样本移动装置802与控制装置连接。
需要说明的是，样本移动装置802可以包括有用于带动样本针801前后移动的样本前后运动机械臂8022和用于带动样本针801升降运动的样本垂直运动机械臂8021，另外，还可以设置第一前后推手803，该第一前后推手用于与提取条转移抓手4配合使用，以实现提取条1的三维空间运动，确保提取条1可移动至加注装置8的加注位。因此，第一前后推手可以从提取条转移抓手4上将提取条1推到加注装置8内，待完成了加注操作后，再将提取条1推回提取条转移抓手4，而后，提取条转移抓手4可将提取条1转移至低温裂解装置9的前方。
另外，需要说明的是，样本针801的底部垂直设有TIP适配器，TIP适配器用来获取提取条1上的2ml TIP108。当第一前后推手将提取条1推到加注装置8内，样本针801可以从提取条1上获取2ml TIP108，样本针801向前移动到样本架载入装置3的抽样处307上方，而后，样本移动装置802带动样本针801移动，以抽取待测样本，并将样本添加到提取条1的反应仓109内，之后，样本针801再从提取条1上的试剂孔内抽取所需试剂添加到反应仓109内，所需试剂包括有：反应需要的磁珠107和核酸释放液106，再将反应仓109的液体吸打混匀，以完成加注操作。最后，样本针801将2ml TIP108退到提取条1上。
可以在实际运用过程中，根据实际情况和实际需求，对样本针801和样本移动装置802的形状、结构、尺寸、位置等进行确定。
需要说明的是，核酸提取装置还可以包括低温裂解装置9，因为样本液一般需要先经过低温裂解装置9的低温温育操作，低温温育操作有利于核酸和磁珠107结合，再经过磁珠清洗装置10对磁珠107进行清洗操作， 以将核酸和磁珠107结合体之外的其他物质去除，而后，再经过高温解离装置11的高温温育操作，高温温育操作有利于核酸和磁珠107分离。但是，由于核酸释放液106的不同，有时会发生无需经过低温裂解装置9的低温温育操作的情况，此时，可以控制提取条转移抓手4将提取条1从加注装置8直接转移到磁珠清洗装置10的前方，无需转移至低温裂解装置9。
优选的，高温解离装置11包括用于对提取条1的反应仓109加热的温育加热块902，温育加热块902与控制装置连接。
需要说明的是，可以设置与温育加热块902连接的加热块驱动装置，且加热块驱动装置与控制装置连接，加热块驱动装置用于驱动温育加热块902进行空间移动，以使加热块靠近或远离反应仓109。
还需要说明的是，低温裂解装置9和高温解离装置11的结构相似，主要是温育加热块902的加热温度有所区别，低温裂解装置9的加热温度较低，而高温解离装置11的加热温度较高。当需要对反应仓109进行温育操作时，可以控制加热块驱动装置运行，以驱动温育加热块902靠近反应仓109，对反应仓109进行加热操作，当温育操作结束后，再控制温育加热块902与反应仓109分离即可。温育加热块902加热过程中，样本液在核酸释放液106的作用下会释放出核酸，并且，核酸会附着到磁珠107上。
另外，需要说明的是，低温裂解装置9可以配置有第二前后推手901，该第二前后推手901用于与提取条转移抓手4配合使用，以实现提取条1的三维空间运动。因此，第二前后推手901可以从提取条转移抓手4上将提取条1推到低温裂解装置9内，待低温温育操作完成后，再将提取条1推回至提取条转移抓手4，提取条转移抓手4再将提取条1转移到磁珠清洗装置10的前方。
优选的，磁珠清洗装置10包括：洗液针1001、用于驱动洗液针1001进行空间移动的洗液驱动装置1002、用于选择性的对提取条1的反应仓109施加磁场的磁体装置1003以及用于驱动磁体装置1003转动的磁体驱动装置；磁体驱动装置和磁体装置1003连接，洗液针1001与洗液驱动装置1002连接；磁体驱动装置和洗液驱动装置1002均与控制装置连接。
需要说明的是，磁珠清洗装置10可以配置有第四前后推手1004，该 第四前后推手1004用于与提取条转移抓手4配合使用，以实现提取条1的三维空间运动。因此，第四前后推手1004可以从提取条转移抓手4上将提取条1推到磁珠清洗装置10内进行磁吸操作。并且，洗液针1001在垂直方向上设有TIP适配器，TIP适配器用来获取提取条1上的2ml TIP108。
在进行磁吸操作时，首先，可使得提取条1上2ml TIP108对准洗液针1001，洗液针1001向下运动以获取2ml TIP108；而后，将提取条1移动到磁吸位，通过转动磁体装置1003，使得第一磁体或第二磁体中的一者转到磁吸位，以使提取条1的反应仓109内的附着有核酸的磁珠107在磁力作用下积聚在反应仓109的侧壁上，磁吸时，提取条1的反应仓109位于洗液针1001的下方，洗液针1001向下将反应仓109内的液体抽走，磁珠107在磁力作用下留在反应仓109内壁，而后，旋转该磁体，使磁体离开磁吸位，并且需要避开提取条1的运动空间；之后，推动提取条1，使提取条1上的空仓对准洗液针1001，洗液针1001将2ml TIP108内所吸取的液体吐出；推动提取条1，使提取条1上放置洗液105的试剂仓对准洗液针1001，洗液针1001吸取洗液105；推动提取条1，使提取条1上的反应仓109对准洗液针1001，洗液针1001将2ml TIP108中的洗液105添加到反应仓109内，并将反应仓109内的磁珠107和洗液105吸打混匀；而后，再将第一磁体或第二磁体中的一者转到磁吸位，使提取条1的反应仓109内的附着有核酸的磁珠107在磁力作用下积聚在反应仓109侧壁上；洗液针1001向下把反应仓109内的液体抽走，磁珠107在磁力作用下留在反应仓109内壁，再旋转磁体，使磁体离开磁吸位，并避开提取条1运动空间；再推动提取条1，使提取条1上的空仓对准洗液针1001，洗液针1001将2ml TIP108内所吸取的液体吐出；而后，可以根据测试项目的需求，对磁珠107进行多次清洗，清洗后的废液吐到提取条1上的空仓中，而清洗后的磁珠107留在反应仓109内；待磁珠107清洗完成后，推动提取条1，使提取条1上2ml TIP108的放置位对准洗液针1001，洗液针1001将2ml TIP108退到提取条1上；再推动提取条1，使提取条1上300ul TIP102的放置位对准洗液针1001，洗液针1001向下获取300ul TIP102；继续推动提取条1，使提取条1上的解离试剂103对准洗液针1001，洗液针1001吸取解离试 剂103，解离试剂103的作用是使附着在磁珠107上的核酸与磁珠107分离；之后，再推动提取条1，使提取条1上的反应仓109对准洗液针1001，洗液针1001将300ul TIP102中的解离试剂103加到反应仓109内，并将反应仓109内的液体吸打混匀；再推动提取条1，使提取条1上300ul TIP102的放置位对准洗液针1001，洗液针1001将300ul TIP102退到提取条1上，继而完成了整个磁吸操作过程。
待磁吸操作完成后，第四前后推手1004将提取条1推回至提取条转移抓手4，提取条转移抓手4再将提取条1转移到高温解离装置11的前方。
优选的，高温解离装置11可以配置有第三前后推手1101，第三前后推手1101用于与提取条转移抓手4配合使用，以实现提取条1的三维空间运动。因此，第三前后推手1101可以从提取条转移抓手4上将提取条1推到高温解离装置11内进行高温温育，在解离试剂103的作用下，通过一定时间的高温温育，可以使附着在磁珠107上的核酸与磁珠107分离，待高温温育操作完成后，再将提取条1推回至提取条转移抓手4，提取条转移抓手4再将提取条1转移到提纯液磁吸装置6的前方。
优选的，提纯液磁吸装置6可以布置在试剂针12的运动范围内，以方便试剂针12吸取提取条1的反应仓109内的提纯液。提纯液磁吸装置6可以配置有提纯前后推手601和磁铁602。其中，提纯前后推手601用于与提取条转移抓手4配合使用，以实现提取条1的三维空间运动。因此，提纯前后推手601可以从提取条转移抓手4上将提取条1推到提纯液磁吸装置6内进行提纯操作，待提纯操作完成后，再将提取条1推回提取条转移抓手4，提取条转移抓手4再将提取条1转移至废弃装置18的前方。磁铁602用于向提取条1的反应仓109施加磁力，以使反应仓109内的磁珠107在磁力作用下积聚在反应仓109侧壁上，使得核酸留在液体中，进而得到检测所需的核酸提纯液。
在上述实施例的基础上，优选的，还包括具有制冷功能的、用于提供不同试剂的冷藏试剂供给装置16。
需要说明的是，冷藏试剂供给装置16配置有制冷功能，是为了更好的保持需要冷藏的试剂，并且，可以在冷藏试剂供给装置16的顶部设有吸液 孔1601，吸液孔1601与试剂针12的尺寸相配合，以便于试剂针12吸取试剂。
优选的，本系统还可以包括用于提供TIP1703和PCR管1704以及PCR盖1705的耗材供给装置17，所述耗材供给装置17设有用于放置TIP1703的TIP耗材架1701和用于放置PCR管1704与PCR盖1705的PCR耗材架1702。
优选的，TIP1703和PCR盖1705的上方均设有内孔，内孔与试剂针12的尺寸相配合，以便于试剂针12取TIP1703和取PCR盖1705。其中，TIP1703和PCR盖1705上方的内孔均可以和试剂针12的TIP适配器孔相匹配，以便于试剂针12取TIP1703和取PCR盖1705。
另外，需要补充说明的是，试剂针12可以采用水平、前后和垂直三维运动机械臂的结构实现空间运动，试剂针12可以在垂直方向设置TIP适配器，TIP适配器用来获取TIP1703或PCR盖1705，试剂针12的运动范围覆盖有：提纯液磁吸装置6、封膜刺破装置7、耗材供给装置17、冷藏试剂供给装置16等，试剂针12获取TIP1703后，可以将冷藏试剂供给装置16上的试剂添加到提取条1的反应仓109或PCR管1704内，将反应仓109的核酸提纯液添加到PCR管1704内，并获取PCR盖1705，将PCR盖1705扣合PCR管1704进行密封，将密封后的PCR管1704转移到PCR管传输装置14上。
例如，试剂针12可以从耗材供给装置17获取TIP1703，从提取条1的反应仓109中吸取核酸提纯液，再将核酸提纯液添加到耗材供给装置17上的PCR管1704内，从TIP废弃口废弃TIP1703。试剂针12从耗材供给装置17获取TIP1703后，从冷藏试剂供给装置16上吸取参与反应所需的试剂，并将该试剂添加到同一PCR管1704内，再从TIP废弃口废弃TIP1703。而后，试剂针12从耗材供给装置17获取PCR盖1705，并将PCR盖1705扣到添加过核酸提纯液和试剂的PCR管1704上。最后，试剂针12再将密封后的PCR管进行转移。
优选的，试剂针12可以包括针装置1201和用于驱动针装置1201进行空间运动的针驱动装置，针装置1201和针驱动装置均与控制装置连接。针 驱动装置可以包括有用于带动针装置1201水平运动的针水平运动机械臂12021、用于带动针装置1201前后运动的针前后运动机械臂12022以及用于带动针装置1201垂直运动的针垂直运动机械臂12023，以实现针装置1201的三维空间运动。
在上述实施例的基础上，优选的，还包括用于回收废弃的提取条1的废弃装置18，废弃装置18与控制装置连接。
优选的，废弃装置18可以包括：用于吸取提取条1的废液的废液针1801、可开合的用于抓取废弃的提取条1的开合抓手1802、用于驱动开合抓手1802张开或闭合的开合驱动装置以及用于驱动废液针1801升降运动的升降驱动装置，开合驱动装置和升降驱动装置均与控制装置连接。
需要说明的是，当试剂针12将提取条1的核酸提纯液添加至PCR管1704后，可以对该提取条1进行废弃操作。废弃装置18可以配置有第五前后推手1803。第五前后推手1803用于与提取条转移抓手4配合使用，以实现提取条1的三维空间运动。因此，第五前后推手1803可以从提取条转移抓手4上将提取条1推到废弃装置18内，而后，提取条1上的反应仓109和各试剂仓依次推到废液针1801下方，废弃针可在垂直方向移动，以将反应仓109和各试剂仓剩余的液体抽走，待抽液操作完成后，第五前后推手1803将提取条1推到开合抓手1802的废弃处，最后，开合抓手1802张开，以在该处废弃提取条1。
在上述实施例的基础上，优选的，PCR检测装置包括用于对密封后的PCR管1704进行不同项目的荧光PCR检测的荧光PCR检测装置13，荧光PCR检测装置13与控制装置连接。
优选的，PCR检测装置还可以包括PCR管传输装置14和PCR管转移抓手15，PCR管传输装置14和PCR管转移抓手15均与控制装置连接。其中，PCR管传输装置14用于将密封后的PCR管1704转移至PCR管转移抓手15的抓取位，PCR管转移抓手15用于将PCR管1704由PCR管传输装置14转移至荧光PCR检测装置13。
需要说明的是，试剂针12可以将密封后的PCR管转移到PCR管传输装置14上。PCR管传输装置14配置有可水平移动的传输块1402，PCR 管1704可以放置在传输块1402上方的PCR管1704放置孔1401内，之后，PCR管1704可以转移至PCR管转移抓手15的抓取位。
优选的，荧光PCR检测装置13包括：壳体、测光组件1301以及温控部件，壳体上设有用于放置PCR管1704的若干测试位1303，各测试位1303均对应设有温控部件，以使各测试位1303均可相互独立的进行温度升降控制，测光组件1301和温控部件均与控制装置连接；测光组件1301包括用于提供测试光源的光源组件以及用于带动光源组件运动的驱动部件；光源组件的个数为多个，各光源组件可提供的光源类型不同，且各光源组件可在驱动部件的带动下照射各测试位1303。
需要说明的是，荧光PCR检测装置13还可以包括用于压紧PCR管1704的压筒1302，壳体上设有磁吸部件，压筒1302上也设置有磁铁件，以使压筒1302吸附在壳体上，从而压紧PCR管。由于每个测试位1303均对应设有用于控制PCR管1704温度的温控部件，因此，每个测试位1303可独立控制温度升降情况，从而实现了不同测试项目的同时在线检测。
还需要说明的是，测光组件1301和温控部件均与控制装置连接，因此，荧光PCR检测装置13在温度升降的同时可以进行实时的荧光检测。并且，每个测试位1303配置有独立的压筒1302，压筒1302具有压紧PCR管1704的作用，压筒1302依靠磁吸力实现快速安装固定，并且可确保PCR管1704与温控部件的配合紧密，以提高检测效果。
另外，需要说明的是，PCR管转移抓手15可进行三维空间的移动，其用于抓取密封后的PCR管1704或压筒1302，以便于配合荧光PCR检测装置13进行检测操作。其中，PCR管转移抓手15可以包括用于抓取密封后的PCR管1704和压筒1302的PCR抓手1501、用于带动PCR抓手1501水平运动的PCR水平运动机械臂1502、用于带动PCR抓手1501前后运动的PCR前后运动机械臂1503以及用于带动PCR抓手1501垂直运动的PCR垂直运动机械臂1504。其中，PCR抓手1501可以抓取荧光PCR检测装置13上的压筒1302，将压筒1302放置到压筒放置处1304，从PCR管传输装置14上抓取PCR管1704放置到荧光PCR检测装置13上的打开了压筒1302的测试位1303，再从压筒放置处1304抓取压筒1302放在PCR管1704 上，待荧光PCR检测完成后，抓取压筒1302放置到压筒放置处1304，抓取检测后的PCR管1704，并将PCR管1704移动至PCR管废弃口1305进行废弃，再从压筒放置处1304抓取压筒1302，将压筒1302放回初始位置。
另外，需要说明的是，本发明所提供的核酸提取及荧光PCR检测系统，其包括框架和分别设置在框架上的提取条载入装置2、样本架载入装置3、提取条转移抓手4、封膜刺破装置7、加注装置8、低温裂解装置9、磁珠清洗装置10、高温解离装置11、提纯液磁吸装置6、废弃装置18、耗材供给装置17、冷藏试剂供给装置16、试剂针12、PCR管传输装置14、PCR管转移抓手15、荧光PCR检测装置13等。将各装置集成到一个系统，使得系统的一体化程度高，可以实现核酸自动化提取和荧光PCR自动检测。
其中，本系统通过提取条载入装置2实现了提取试剂的自动载入；通过样本架载入装置3实现了待测样本的自动载入和载出；通过提取条转移抓手4、封膜刺破装置7、加注装置8、低温裂解装置9、磁珠清洗装置10、高温解离装置11、提纯液磁吸装置6、废弃装置18、耗材供给装置17、冷藏试剂供给装置16以及加注装置8，实现了核酸的自动化提取操作；通过PCR管传输装置14、PCR管转移抓手15以及荧光PCR检测装置13，实现了核酸的荧光PCR自动检测操作。
本系统支持待测样本随时上机检测，配置了多个独立的温升降测光装置，支持多个项目的同时在机检测，实现了待测样本单个随机、随来随检功能。有效减少了样本的检测等待时间。此外，本系统的核酸提取、荧光PCR检测全程均无人工参与，自动化程度高，可有效避免检测时的人工失误，提高检测效率、降低劳动强度。
需要进行说明的是，本申请文件中提到的第一通道201和第二通道202、第一推手装置203和第二推手装置204、第一磁体和第二磁体、第一前后推手803和第二前后推手901和第三前后推手1101和第四前后推手1004以及第五前后推手1803，其中，第一和第二和第三和第四以及第五只是为了区分位置的不同，并没有先后顺序之分。
另外，还需要说明的是，本申请的“上下”、“前后”等指示的方位或位置关系，是基于附图所示的方位或位置关系，仅是为了便于简化描述和 便于理解，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。
本说明书中各个实施例采用递进的方式描述，每个实施例重点说明的都是与其他实施例的不同之处，各个实施例之间相同相似部分互相参见即可。本发明所提供的所有实施例的任意组合方式均在此发明的保护范围内，在此不做赘述。
以上对本发明所提供的核酸提取及荧光PCR检测系统进行了详细介绍。本文中应用了具体个例对本发明的原理及实施方式进行了阐述，以上实施例的说明只是用于帮助理解本发明的方法及其核心思想。应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以对本发明进行若干改进和修饰，这些改进和修饰也落入本发明权利要求的保护范围内。

Claims (14)

  1. 一种核酸提取及荧光PCR检测系统，其特征在于，包括：提取条(1)、具有在线添加所述提取条(1)功能的用于载入所述提取条(1)的提取条载入装置(2)、用于载入和载出样本的样本架载入装置(3)、用于带动所述提取条(1)移动至预设位置的提取条转移抓手(4)、用于对所述样本进行提纯以得到核酸的核酸提取装置、用于对所述核酸进行荧光PCR检测的PCR检测装置以及控制装置；
    所述提取条载入装置(2)、所述样本架载入装置(3)、所述提取条转移抓手(4)、所述核酸提取装置以及所述PCR检测装置均与所述控制装置连接。
  2. 根据权利要求1所述的核酸提取及荧光PCR检测系统，其特征在于，所述提取条载入装置(2)包括：通道和用于推动所述提取条(1)在所述通道内移动的推手装置；
    所述通道的一端为所述提取条(1)的抓取处(207)，所述推手装置可横向移动，所述推手装置与所述控制装置连接。
  3. 根据权利要求2所述的核酸提取及荧光PCR检测系统，其特征在于，所述抓取处(207)设有可升降的用于分离所述提取条(1)的顶升装置(208)，所述顶升装置(208)与所述控制装置连接。
  4. 根据权利要求1至3任一项所述的核酸提取及荧光PCR检测系统，其特征在于，所述样本架载入装置(3)包括：用于装载样本的样本架(301)、载入推手(302)、急诊推手(303)、扫描仪(304)、转移推手(305)以及载出推手(306)，所述转移推手(305)用于将样本转移至所述扫描仪(304)的扫描处、并将扫描后的样本移动至抽样处(307)，所述载出推手(306)用于将抽样后的样本运回样本回收区；
    所述载入推手(302)、所述急诊推手(303)、所述扫描仪(304)、所述转移推手(305)以及所述载出推手(306)均与所述控制装置连接。
  5. 根据权利要求1至3任一项所述的核酸提取及荧光PCR检测系统，其特征在于，所述提取条转移抓手(4)包括：可开合的用于抓取所述提取条(1)的抓手装置(401)、用于带动所述抓手装置(401)升降运动的抓 手升降装置(402)以及用于驱动所述抓手升降装置(402)横向移动的抓手横移装置(403)，所述抓手装置(401)设于所述抓手升降装置(402)上，所述抓手升降装置(402)和所述抓手横移装置(403)滑动连接；
    所述抓手装置(401)、所述抓手升降装置(402)以及所述抓手横移装置(403)均与所述控制装置连接。
  6. 根据权利要求1至3任一项所述的核酸提取及荧光PCR检测系统，其特征在于，所述核酸提取装置包括：用于将所述提取条(1)的封膜刺破的封膜刺破装置(7)、用于将待测的所述样本添加至所述提取条(1)的加注装置(8)、试剂针(12)、磁珠清洗装置(10)、用于对所述样本进行高温温育操作的高温解离装置(11)以及用于对所述样本进行磁吸提纯以得到核酸提纯液的提纯液磁吸装置(6)；
    所述试剂针(12)用于将对应试剂添加至所述提取条(1)或PCR管(1704)内和用于将所述提取条(1)的核酸提纯液添加至所述PCR管(1704)内。
  7. 根据权利要求6所述的核酸提取及荧光PCR检测系统，其特征在于，所述封膜刺破装置(7)包括：刺破器(701)和驱动组件，所述驱动组件与所述刺破器(701)连接，以驱动所述刺破器(701)移动，当所述刺破器(701)移动到刺破工位，所述刺破器(701)将所述提取条(1)的封膜刺破，所述驱动组件与所述控制装置连接。
  8. 根据权利要求6所述的核酸提取及荧光PCR检测系统，其特征在于，所述加注装置(8)包括：用于吸取所述样本的样本针(801)和用于驱动所述样本针(801)进行空间移动的样本移动装置(802)，所述样本针(801)与所述样本移动装置(802)连接，所述样本移动装置(802)与所述控制装置连接。
  9. 根据权利要求6所述的核酸提取及荧光PCR检测系统，其特征在于，所述高温解离装置(11)包括用于对所述提取条(1)的反应仓(109)加热的温育加热块(902)，所述温育加热块(902)与所述控制装置连接。
  10. 根据权利要求6所述的核酸提取及荧光PCR检测系统，其特征在于，所述磁珠清洗装置(10)包括：洗液针(1001)、用于驱动所述洗液针 (1001)进行空间移动的洗液驱动装置(1002)、用于选择性的对所述提取条(1)的反应仓(109)施加磁场的磁体装置(1003)以及用于驱动所述磁体装置(1003)转动的磁体驱动装置；
    所述磁体驱动装置和所述磁体装置(1003)连接，所述洗液针(1001)与所述洗液驱动装置(1002)连接；
    所述磁体驱动装置和所述洗液驱动装置(1002)均与所述控制装置连接。
  11. 根据权利要求1至3任一项所述的核酸提取及荧光PCR检测系统，其特征在于，还包括具有制冷功能的、用于提供不同试剂的冷藏试剂供给装置(16)。
  12. 根据权利要求1至3任一项所述的核酸提取及荧光PCR检测系统，其特征在于，还包括用于回收废弃的所述提取条(1)的废弃装置(18)；所述废弃装置(18)与所述控制装置连接。
  13. 根据权利要求1至3任一项所述的核酸提取及荧光PCR检测系统，其特征在于，所述PCR检测装置包括用于对密封后的所述PCR管(1704)进行不同项目的荧光PCR检测的荧光PCR检测装置(13)；
    所述荧光PCR检测装置(13)与所述控制装置连接。
  14. 根据权利要求13所述的核酸提取及荧光PCR检测系统，其特征在于，所述荧光PCR检测装置(13)包括：壳体、测光组件(1301)以及温控部件，所述壳体上设有用于放置所述PCR管(1704)的若干测试位(1303)，各所述测试位(1303)均对应设有所述温控部件，以使各所述测试位(1303)相互独立的进行温度升降控制，所述测光组件(1301)和所述温控部件均与所述控制装置连接；
    所述测光组件(1301)包括用于提供测试光源的光源组件以及用于带动所述光源组件运动的驱动部件；所述光源组件的个数为多个，各所述光源组件可提供的光源类型不同，且各所述光源组件可在所述驱动部件的带动下照射各所述测试位(1303)。
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