CN108998370A - The susceptibility automatic incubation system of microorganism - Google Patents

The susceptibility automatic incubation system of microorganism Download PDF

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Publication number
CN108998370A
CN108998370A CN201811088104.7A CN201811088104A CN108998370A CN 108998370 A CN108998370 A CN 108998370A CN 201811088104 A CN201811088104 A CN 201811088104A CN 108998370 A CN108998370 A CN 108998370A
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China
Prior art keywords
incubator
incubation
plate
microorganism
automatic
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Pending
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CN201811088104.7A
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Chinese (zh)
Inventor
段力争
吉鸿超
徐真
郑业焕
余峰
刘聪
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Autobio Experimental Instrument Zhengzhou Co Ltd
Autobio Labtec Instruments Zhengzhou Co Ltd
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Autobio Labtec Instruments Zhengzhou Co Ltd
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Priority to CN201811088104.7A priority Critical patent/CN108998370A/en
Publication of CN108998370A publication Critical patent/CN108998370A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of susceptibility automatic incubation systems of microorganism, including incubator, and are set in incubator for storing the incubation mechanism of drug sensitive plate, and board is provided on the side wall of incubator and picks and places mouth;The sidewall bottom of incubator is communicated with horizontally disposed circulation air path, and the circulating fan of electrified heating silk is provided at the air outlet of circulation air path, temperature sensor is provided in incubator.The invention has the advantages that structure is simple, easy to operate, stability is good, and support plate amount is big, high degree of automation.

Description

The susceptibility automatic incubation system of microorganism
Technical field
The present invention relates to microorganism detection fields, more particularly, to a kind of susceptibility automatic incubation system of microorganism.
Background technique
With the continuous development of science and technology, requirement of the people to medical treatment detection device the degree of automation is also higher and higher; Whether, in microorganism detection field, microorganism incubation system is widely used to clinical detection, deposit for detecting in biological sample In microorganism, the type and quantity of microorganism are predominantly detected.Since the individual of microorganism is invisible to the naked eye, and to rings such as temperature Border requirement is particularly severe, therefore generally needs first to be cultivated with constant-temperature incubation case before carrying out microorganism detection;Current perseverance All kinds of corrupt practices creep in for warm incubator culture, and such as storage board limited amount, board pick-and-place program are cumbersome, Manual operation link excessively increases The big probability etc. of cross contamination, causes culture efficiency very low, influences the development of this field.
Summary of the invention
Operate that convenient, stability is good, support plate amount is big, the susceptibility of high degree of automation use it is an object of that present invention to provide a kind of The automatic incubation system of microorganism.
To achieve the above object, the present invention can take following technical proposals:
The susceptibility of the present invention automatic incubation system of microorganism, including incubator, and be set in the incubator and use In the incubation mechanism of storage drug sensitive plate, it is provided with board on the side wall of the incubator and picks and places mouth;The incubation mechanism includes It is fixedly arranged on the pedestal with supporting leg in the incubator inner bottom wall, being horizontally disposed in the upper surface of the pedestal has thin-wall bearing, institute The inner ring and pedestal for stating thin-wall bearing are fixedly connected with, and the driven pulley driven by driving motor is installed on outer ring, described driven The upper surface of belt wheel is fixedly connected with the horizontal disk that is incubated for coaxially laid, and is distributed along the circumference with several groups on the horizontal incubation disk and is opened Mouth incubation pylon outwardly, longitudinal gap is arranged with several layers drug sensitive plate card slot in the incubation pylon, and every group of incubation pylon is equal It is fixed by a pin to horizontal incubation disk upper surface;
The board pick and place mouth be provided with respectively on the incubator side wall into plate mouth and plate outlet, it is described into plate mouth be vertical What is opened up to interval is several into plate door opening, described to correspond into plate door opening and the drug sensitive plate card slot, into leading on plate door opening It crosses automatic rebounding hinge and is hinged with and push;The size of the plate outlet is corresponding with the outward opening size adaptation of pylon is incubated for, and is going out It is hinged on plate mouth and takes plate door, be provided with several incubations in the corresponding plate outlet opening's edge in plate door enabling side with described take and believe Indicator light is ceased, and controls starting switch for the incubation information indicator light unlatching, the incubation information indicator light and the susceptibility Board slot corresponds;
The sidewall bottom of the incubator is communicated with horizontally disposed circulation air path, is arranged at the air outlet of the circulation air path There is the circulating fan of electrified heating silk, is provided with temperature sensor in the incubator.
It is provided with speed changer on the base, the driving motor is driven by the speed changer and the driven pulley Connection;The speed changer is fixed in the base lower surface by fixed plate, and the output end of speed changer extends vertically up to Above pedestal, it is provided with driving pulley, the driving pulley is sequentially connected by transmission belt and the driven pulley, speed change The input terminal of device is sequentially connected by synchronous belt drive mechanism and the driving motor.
Position sensor is set on the pedestal.
The horizontal upper surface for being incubated for disk is circumferentially arranged to be had and the one-to-one pylon scale of incubation pylon.
The upper surface for being incubated for pylon is provided with lift-pull handle.
The pedestal is fixed in the incubator inner bottom wall by being set to the fixing seat of the supporting leg lower end.
Sealing, which is worn, on the roof of the incubator has penetrated thermometer, positioned at the incubator side wall taken above plate door On be provided with for showing the display screen for incubating information inside incubator.
The enabling side for taking plate door, which is provided with, consolidates magnetic support with the attracted suction of the plate outlet, inhales solid magnetic close to described Hall sensor is provided on plate outlet at seat.
The ring packing adhesive tape that fitting is adapted to the plate outlet opening's edge is provided on the medial surface for taking plate door.
The invention has the advantages that structure is simple, easy to operate, stability is good, and support plate amount is big, high degree of automation.This is automatic Incubation system can also be provided on incubator side wall separately as microbial cultivation instrument (constant-temperature incubation case) by longitudinal gap With drug sensitive plate card slot correspondingly into the external biological sample adding system of plate door opening cooperation, form full automatic microorganism identification and analysis Instrument, realizes the automation of susceptibility board fetching process, and then reaches the full-automation of entire instrument, effectively improves work effect Rate reduces artificial participation and manually puts plate link, reduces cross contamination probability;Level, which is incubated on disk, is evenly distributed multiple groups incubation Pylon, longitudinal gap arranges multilayer drug sensitive plate card slot in every group of incubation pylon, substantially increases the support plate amount of system, design is more It is exquisite.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 is the A direction view of Fig. 1.
Fig. 3 is the structural schematic diagram that mechanism is incubated in Fig. 1.
Fig. 4 is the B direction view of Fig. 3.
Specific embodiment
As shown in Figure 1, the susceptibility of the present invention automatic incubation system of microorganism, including incubator 1, and be set to For storing the incubation mechanism of drug sensitive plate 2 in incubator 1, it is provided with board on the side wall of incubator 1 and picks and places mouth, board picks and places Mouthful for be provided with respectively on 1 side wall of incubator into plate mouth 11 and plate outlet 12, drug sensitive plate 2 is put into from into plate mouth 11, from ejecting plate Mouth 12 takes out.
As shown in Figure 3,4, being incubated for mechanism includes the pedestal 4 being fixedly arranged in 1 inner bottom wall of incubator with supporting leg 3, and pedestal 4 passes through The fixing seat 29 for being set to 3 lower end of supporting leg is fixed in 1 inner bottom wall of incubator;Being horizontally disposed in the upper surface of pedestal 4 has thin-walled Bearing 5, inner ring and the pedestal 4 of thin-wall bearing 5 are fixedly connected with, and the driven pulley 7 driven by driving motor 6 is installed on outer ring, The upper surface of driven pulley 7 is fixedly connected with the horizontal disk 8 that is incubated for coaxially laid, and level is incubated on disk 8 and is distributed along the circumference with eight groups The box-structure of opening outwardly is incubated for pylon 9, is incubated for longitudinal gap in pylon 9 and is arranged with eight layers of drug sensitive plate card slot 10, total can 64 drug sensitive plates 2 disposably are put into, substantially increase support plate amount.
To make horizontal be incubated on disk 8 that can be evenly distributed with the identical incubation pylon 9 of eight groups of structures, level is incubated for the diameter meeting of disk 8 It is relatively large, cause 7 outer diameter of driven pulley for driving it to rotate larger, and existing driving motor 6 is mostly fixed rotating speed, therefore For the revolving speed for being adapted to driving motor 6, speed changer 21 is provided on pedestal 4, driving motor 6 passes through speed changer 21 and driven pulley 7 transmission connections;Specifically, speed changer 21 is fixed on 4 lower surface of pedestal by fixed plate 22, the output end of speed changer 21 is vertical 4 top of pedestal is extended upwardly to, driving pulley 23 is provided with, driving pulley 23 is passed by transmission belt 24 and driven pulley 7 Dynamic connection, and the input terminal of speed changer 21 is sequentially connected by another synchronous belt drive mechanism 25 with driving motor 6.
It is incubated for pylon 9 for convenience of picking and placing, easy to repair, every group of incubation pylon 9 is fixed by a pin to horizontal incubation disk 8 Upper surface, and the upper surface for being incubated for pylon 9 is provided with lift-pull handle 28;In addition, for convenience of can be with when picking and placing drug sensitive plate 2 The position for fast and accurately finding required drug sensitive plate 2 has and is incubated for pylon 9 the horizontal upper surface for being incubated for disk 8 is circumferentially arranged One-to-one pylon scale 27, while position sensor 26 is set on pedestal 4, disk 8, which is incubated for, for recording level rotates Location information, guarantee tower position running accuracy.
In order to cooperate with existing automatic sample-adding system, it is conveniently automatically put into drug sensitive plate 2, as shown in Figure 1, being into plate mouth 11 Open up eight of longitudinal gap correspond into plate door opening into plate door opening and drug sensitive plate card slot 10, on plate door opening by from Dynamic rebound hinge 13, which is hinged with, pushes 14, and automatic rebounding hinge 13 is that (full name is that torsion spring type hydraulic cushion is closed and closed to hydraulic hinge Page) or pneumatic hinge (full name is pneumatic type automatic door-closing hinge), when guaranteeing that manipulator exits station, push 14 rebounds, into plate Door opening closure, effectively reduces the manual intervention in experimentation, avoids manually putting cross contamination caused by plate, reduce shakiness Determine risk of failure caused by factor.
The size of plate outlet 12 is corresponding with the outward opening size adaptation of pylon 9 is incubated for, and is hinged on plate outlet 12 and takes plate Door 15 is provided with suitable with 12 opening's edge of plate outlet to enhance the leakproofness for taking plate door 15 on the medial surface for taking plate door 15 Ring packing adhesive tape 34 with fitting;In addition, being also provided in the enabling side for taking plate door 15 attracted with plate outlet 12 Solid magnetic support 32 is inhaled, and Hall sensor 33 is being set on the plate outlet 12 inhaled at solid magnetic support 32, when taking plate door 15 not complete When shutting entirely, Hall sensor 33 can issue alarm signal, prompt that plate door 15 is taken not shut.
Several incubation information indicator lights 16 are provided in 12 opening's edge of plate outlet corresponding with 15 enabling side of plate door is taken, and What control incubation information indicator light 16 was opened starts switch 17, incubates information indicator light 16 and drug sensitive plate card slot 10 corresponds, When taking plate door 15 to open, 17 unlatchings are started switch, the energization of information indicator light 16 is incubated and lights, incubating information indicator light 16 can Realize that different colors is shown by related software, the corresponding different incubation information for being incubated for drug sensitive plate 2 in pylon 9;In addition, incubating It educates sealing on the roof of case 1 and wears thermometer 30, operator is facilitated to read temperature information, meanwhile, it is taking above plate door 15 1 side wall of incubator on setting for show inside incubator 1 incubate information display screen 31, can show at any time in incubator 1 The incubation information in portion, facilitates operator to grasp the real-time parameter in incubator 1 in time.
As shown in Fig. 2, the sidewall bottom in incubator 1 is communicated with horizontally disposed circulation air path 18, in circulation air path 18 Air outlet at be provided with the circulating fan 20 of electrified heating silk 19, circulating fan 20 is dried into incubator 1, realizes internal temperature That spends is constant;In addition, temperature sensor is additionally provided in incubator 1, by temperature sensor come the incubation of real-time monitoring temperature 1 internal temperature of case, after temperature reaches desirable value in incubator 1, electric heating wire 19 can be automatically stopped work, and then realize temperature Automatic control.

Claims (10)

1. a kind of susceptibility automatic incubation system of microorganism, including incubator (1), and be set in the incubator (1) and use In the incubation mechanism of storage drug sensitive plate (2), it is provided with board on the side wall of the incubator (1) and picks and places mouth;It is characterized by: The mechanism that is incubated for includes the pedestal (4) being fixedly arranged in the incubator (1) inner bottom wall with supporting leg (3), in the pedestal (4) Upper surface is horizontally disposed to be had thin-wall bearing (5), and inner ring and the pedestal (4) of the thin-wall bearing (5) are fixedly connected with, and are covered admittedly on outer ring There are the driven pulley (7) driven by driving motor (6), the upper surface of the driven pulley (7) and the horizontal incubation coaxially laid Disk (8) is fixedly connected with, and horizontal be incubated for is distributed along the circumference with the incubation pylon (9) of several groups opening outwardly on disk (8), described to incubate It educates pylon (9) interior longitudinal gap and is arranged with several layers drug sensitive plate card slot (10), every group of incubation pylon (9) is fixed by a pin to Level is incubated for disk (8) upper surface;
The board pick and place mouth be provided with respectively on the incubator (1) side wall into Ban Kou (11) and plate outlet (12), institute State that be that longitudinal gap opens up into Ban Kou (11) several into plate door opening, it is described into plate door opening and the drug sensitive plate card slot (10) one by one It is corresponding, into being hinged with push (14) by automatic rebounding hinge (13) on plate door opening;The size of the plate outlet (12) with incubate The outward opening size adaptation for educating pylon (9) corresponds to, and is hinged on plate outlet (12) and takes plate door (15), takes plate door with described (15) several incubation information indicator lights (16), and the control incubation are provided in the corresponding plate outlet in enabling side (12) opening's edge What information indicator light (16) was opened starts switch (17), the incubation information indicator light (16) and the drug sensitive plate card slot (10) one One is corresponding;
The sidewall bottom of the incubator (1) is communicated with horizontally disposed circulation air path (18), in the circulation air path (18) It is provided with the circulating fan (20) of electrified heating silk (19) at air outlet, is provided with temperature sensor in the incubator (1).
2. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: on the pedestal (4) It is provided with speed changer (21), the driving motor (6) is sequentially connected by the speed changer (21) and the driven pulley (7).
3. the susceptibility according to claim 2 automatic incubation system of microorganism, it is characterised in that: the speed changer (21) is logical It crosses fixed plate (22) to be fixed on the pedestal (4) lower surface, the output end of speed changer (21) extends vertically up to pedestal (4) Top is provided with driving pulley (23), and the driving pulley (23) is passed by transmission belt (24) and the driven pulley (7) The input terminal of dynamic connection, speed changer (21) is sequentially connected by synchronous belt drive mechanism (25) and the driving motor (6).
4. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: set on the pedestal (4) It is equipped with position sensor (26).
5. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: the horizontal incubation disk (8) upper surface is circumferentially arranged to be had and the one-to-one pylon scale (27) of the incubation pylon (9).
6. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: the incubation pylon (9) Upper surface be provided with lift-pull handle (28).
7. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: the pedestal (4) passes through The fixing seat (29) for being set to the supporting leg (3) lower end is fixed in the incubator (1) inner bottom wall.
8. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: in the incubator (1) Roof on sealing be equipped with thermometer (30), be provided on described incubator (1) side wall taken above plate door (15) For showing the display screen (31) for incubating information inside incubator (1).
9. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: described to take plate door (15) Enabling side be provided with and consolidate magnetic support (32) with the attracted suction of the plate outlet (12), inhale going out at solid magnetic support (32) close to described Hall sensor (33) are provided on plate mouth (12).
10. the susceptibility according to claim 1 automatic incubation system of microorganism, it is characterised in that: take plate door described (15) the ring packing adhesive tape (34) that fitting is adapted to the plate outlet (12) opening's edge is provided on medial surface.
CN201811088104.7A 2018-09-18 2018-09-18 The susceptibility automatic incubation system of microorganism Pending CN108998370A (en)

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Publication number Priority date Publication date Assignee Title
CN109722379A (en) * 2019-01-23 2019-05-07 安图实验仪器(郑州)有限公司 Full automatic microorganism identification and Analysis of Drug Susceptibility system
CN114107039A (en) * 2022-01-28 2022-03-01 珠海迪尔生物工程股份有限公司 Microorganism drug sensitivity analyzer

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109722379A (en) * 2019-01-23 2019-05-07 安图实验仪器(郑州)有限公司 Full automatic microorganism identification and Analysis of Drug Susceptibility system
WO2020151368A1 (en) * 2019-01-23 2020-07-30 安图实验仪器(郑州)有限公司 Fully automatic microorganism identification and drug sensitivity analysis system
CN114107039A (en) * 2022-01-28 2022-03-01 珠海迪尔生物工程股份有限公司 Microorganism drug sensitivity analyzer

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