CN107287109B - Automatic detection system of microorganism incubator and microorganism incubator with same - Google Patents
Automatic detection system of microorganism incubator and microorganism incubator with same Download PDFInfo
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- CN107287109B CN107287109B CN201710614879.2A CN201710614879A CN107287109B CN 107287109 B CN107287109 B CN 107287109B CN 201710614879 A CN201710614879 A CN 201710614879A CN 107287109 B CN107287109 B CN 107287109B
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- 238000013480 data collection Methods 0.000 claims abstract description 4
- 230000011664 signaling Effects 0.000 claims abstract description 3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
The invention discloses an automatic detection system of a microorganism incubator, which comprises: the culture unit comprises a transfer device and a sample tray, wherein the transfer device transfers the incubator to a collection area of the data collection unit according to a transfer instruction; the data acquisition unit is used for acquiring data of the incubator in the acquisition area according to the detection instruction; the data processing unit is used for receiving, storing and analyzing the processed data information; the timing unit starts timing after receiving the timing signal, and sends a timing completion signal after the timing duration reaches the preset duration; and the control unit is used for sending out a transmission instruction and a detection instruction after receiving the timing completion signal, and controlling the culture unit and the data acquisition unit to work. The invention also discloses a microorganism incubator with the automatic detection system. The microbial incubator disclosed by the invention can automatically detect the flora cultured in the microbial incubator on the premise of not opening the microbial incubator, so that the stability of the culture environment in the incubator is ensured.
Description
Technical Field
The invention relates to microorganism culture equipment, in particular to an automatic detection system of a microorganism incubator and the microorganism incubator with the system.
Background
Microorganisms, including bacteria, viruses, fungi, and some small protozoa, microalgae, etc., are a large group of organisms, whose individuals are tiny and closely related to humans. The method covers a plurality of harmful species, and widely relates to various fields of food, medicine, industry and agriculture, environmental protection and the like.
Microorganisms are extremely widely distributed in nature, are large in quantity and various in variety, and can benefit mankind and also become a cause of pathogenicity. Most microorganisms can be cultured by artificial means, i.e., they are inoculated onto a medium and allowed to grow and reproduce. The cultured microorganisms are useful for research, identification and application. Microorganism culture is a complex technique. Microorganism culture is a technology for artificially growing and reproducing microorganisms, and is a conventional technology in life science research.
The culture method and medium should be selected according to the type and purpose of microorganism, and culture conditions (such as temperature, pH, time, and oxygen demand) should be established. The general operation steps are that firstly, the specimen is inoculated on a solid culture medium for separation culture. The single colony obtained is further subjected to morphological, biochemical and serological reaction identification. Since bacteria are ubiquitous, the whole culture process must be performed according to the aseptic operation requirement from the time of preparing the culture medium, otherwise, the sample is polluted by external bacteria, and erroneous results are caused; and once the cultured pathogenic bacteria pollute the environment, cross infection is caused.
In the conventional microorganism culture, the cultured microorganisms are placed on a shelf in an incubator, the microorganisms are cultured under a specific humidity and temperature in the incubator, and the growth of the bacterial flora is manually observed day by day during the culture, and usually 14 days is a culture period. In the process of manually observing the growth of the bacterial flora, the incubator needs to be taken out from the incubator every time, and the incubator needs to be replaced after observation. In the process, external air is brought into the incubator every time the incubator is opened, so that the culture environment in the incubator is unstable, and the growth of colonies is affected; and time and labor are wasted when the growth condition of the flora is observed manually, and meanwhile, misjudgment caused by the human is easy to occur.
Disclosure of Invention
The invention provides an automatic detection system of a microorganism incubator, which can automatically detect bacterial groups cultured in the microorganism incubator on the premise of not opening the microorganism incubator.
An automated detection system for a microbiological incubator, comprising:
the culture unit comprises a transmission device and a sample tray for placing the incubator, the sample tray is linked with the transmission device, and the transmission device transmits the incubator on the sample tray to a collection area of the data collection unit according to a transmission instruction of the control unit;
the data acquisition unit is used for acquiring data of flora in the incubator in the acquisition area according to the detection instruction of the control unit;
the data processing unit is used for receiving and storing the data information acquired by the data acquisition unit and analyzing and processing the data information;
the timing unit is used for triggering to start timing after receiving the timing signal, and sending a timing completion signal to the control unit after the timing time length reaches the preset time length;
and the control unit is used for sending out a transmission instruction and a detection instruction according to a preset program after receiving the timing completion signal, and controlling the culture unit and the data acquisition unit to work.
The working process of the automatic detection system of the invention is as follows: before culturing, manual feeding can be carried out, inoculated incubators are placed on a sample tray one by one, after feeding is completed, a timing unit is triggered to start timing, when a first preset time length is reached, the timing unit sends a timing completion signal to a control unit, the control unit sends a transfer instruction to a transfer device after receiving the timing completion signal, the transfer device transfers the incubators to be detected on the sample tray into a collecting area of a data collecting unit one by one according to the transfer instruction, the control unit sends a detection instruction to the data collecting unit, the data collecting unit collects data of flora in the incubators in the collecting area one by one according to the detection instruction, the collected data information is transmitted to a data processing unit, and the data processing unit stores the data information in real time and analyzes until all incubators on the sample tray are detected; and when the next preset time length is reached, repeating the steps until the culture period is finished.
The automatic detection system is matched with the microorganism incubator for use, and can automatically detect the bacterial colony cultured in the microorganism incubator on the premise of not opening the microorganism incubator.
In order to reduce the size of the sample tray, it is preferable that the sample tray is provided with a plurality of positioning holes for placing the incubator, and the positioning holes are arranged at least twice along the circumferential direction of the sample tray.
The data acquisition unit comprises:
the detection bracket is linked with the linear motor;
the detection mechanism is arranged on the detection bracket and is used for collecting data of flora in the incubator in the collection area;
and the linear motor is used for driving the detection bracket to linearly move relative to the culture unit.
Because the sample tray is provided with a plurality of circles of positioning holes, the incubators are arranged on the sample tray in a plurality of circles, and the data acquisition unit is switched to detect the incubators in different circles by driving the movement of the detection bracket through the linear motor.
For example, the detection mechanism can collect the data of the outer ring incubator first, and after the outer ring incubator is collected, the detection mechanism on the detection bracket is aligned to the inner ring incubator through the movement of the linear motor, and the data collection of the inner ring incubator is switched.
In order to ensure the reliability of detection of different circle layer incubators, preferably, the sample tray comprises an inner circle turntable and an outer circle turntable, and the outer circle turntable is sleeved on the outer edge of the inner circle turntable through a bearing; the motor comprises a first motor linked with the inner ring turntable and a second motor linked with the outer ring turntable.
The bearing is a rolling bearing or a sliding bearing.
The sliding bearing can be made of copper or nonmetallic wear-resistant materials such as Polytetrafluoroethylene (PTFE) \polyphenylene sulfide (PPS) \polyoxymethylene resin (POM) \nylon 66 (PA 66), and the wear resistance of the sliding bearing is improved.
When detecting the incubator on the outer ring turntable, only the outer ring turntable is rotated, the inner ring turntable is kept static through the braking torque of the first motor, and the rotation of the outer ring turntable is not interfered; when detecting the incubator on the inner ring turntable, only the inner ring turntable is rotated, and the outer ring turntable is kept static through the braking torque of the second motor, so that the rotation of the inner ring turntable is not interfered.
Further preferably, the inner ring turntable is fixed on an output shaft of the first motor through a first coupling; the outer edge of the outer ring turntable is provided with meshing teeth, the output shaft of the second motor is fixed with a meshing gear through a second coupler, and the meshing gear is meshed with the meshing teeth of the outer ring turntable.
The outer ring turntable is connected with the inner ring turntable through a bearing, the outer ring turntable and the inner ring turntable are independently rotated through the driving of the first motor and the second motor respectively, and the outer ring turntable and the inner ring turntable are supported on an output shaft of the first motor through a first coupler.
Preferably, the data acquisition unit further comprises a proximity sensor, and the inner ring turntable and the outer ring turntable are respectively provided with an induction area of the proximity sensor.
The proximity sensor is a device having an ability to sense the proximity of an object, and it recognizes the proximity of an object by using the sensitivity characteristic of the displacement sensor to the approaching object, and outputs a corresponding switching signal.
The proximity sensor and the sensing area are used for marking the detection starting point and the detection ending point of the inner ring turntable and the outer ring turntable. For example, during detection, the outer ring turntable is detected firstly, and when the proximity sensor senses the sensing area, the data acquisition device starts to acquire the data of the incubators on the outer ring turntable one by one until the proximity sensor senses the sensing area of the outer ring turntable again, and then the detection of the outer ring turntable is finished; and rotating the inner ring turntable until the proximity sensor senses the sensor of the inner ring turntable, moving the data acquisition unit to a detection area of the inner ring turntable by the linear motor, starting to detect the incubators on the inner ring turntable one by one, and ending the detection of the inner ring turntable until the proximity sensor senses the sensing area of the inner ring turntable again.
In order to further increase the one-time-culturable sample volume of the incubator, it is preferable that the sample tray includes at least two layers coaxially arranged up and down and synchronously rotated; the data acquisition unit at least comprises two sets of detection mechanisms, and the detection mechanisms are in one-to-one correspondence with the sample discs.
The structure of each layer of sample disk is same and rotates synchronously, and when the incubator on the sample disk is detected, each layer of sample disk can be detected simultaneously or respectively.
Preferably, the detection mechanism comprises a laser headspace detector and a camera.
The data information collected by the laser headspace detector and the image information collected by the camera are transmitted to the data processing unit in real time and stored, and the data processing unit further analyzes and processes the stored data information and image information.
The invention also provides a microorganism incubator with the automatic detection system.
Compared with the prior art, the invention has the beneficial effects that:
(1) The automatic detection system can automatically detect the flora cultured in the microbial incubator on the premise of not opening the microbial incubator, ensures the stability of the culture environment in the incubator and reduces the possibility of cross contamination;
(2) The labor can be greatly saved, the laser detection system and the video detection system are adopted, the detection consistency can be increased, and misjudgment caused by manpower can be avoided;
(3) The detection data is transmitted and stored in real time, so that batch tracing of the incubator is facilitated;
(4) Compact structure, high detection efficiency and simple operation.
Drawings
FIG. 1 is a schematic diagram of an automatic inspection system according to the present invention;
FIG. 2 is a schematic diagram of the structure of a sample tray;
FIG. 3 is a cross-sectional view of a sample tray;
FIG. 4 is a schematic diagram of the workflow of the automatic inspection system of the present invention.
Detailed Description
The invention will be described in further detail below with reference to the drawings and examples, it being noted that the examples described below are intended to facilitate an understanding of the invention and are not intended to limit the invention in any way.
The automatic detection system of the microorganism incubator comprises an incubation unit, a data acquisition unit, a data processing unit, a timing unit and a control unit.
As shown in fig. 1 and 2, the culture unit is mounted on the stationary base 5, and includes a first motor 6, a second motor 14, and a sample tray.
The first motor 6 and the second motor 14 are servo motors.
The sample disk comprises an upper layer sample disk and a lower layer sample disk, each layer sample disk is divided into an inner ring turntable 3 and an outer ring turntable 4, the outer ring turntable 4, an inner ring positioning hole 18 and an outer ring positioning hole 17 are respectively arranged on the inner ring turntable 3 and the outer ring turntable 4, and the positioning holes are uniformly distributed along the circumferential direction of the sample disk. The inner ring positioning hole 18 and the outer ring positioning hole 17 are used for placing the inner ring incubator 1 and the outer ring incubator 2, respectively.
In order to reduce the weight of the sample tray, the sample tray is also provided with a turntable lightening hole 19.
As shown in fig. 3, the outer ring turntable 4 is sleeved on the outer edge of the inner ring turntable 3 through a bearing 22, a sliding surface 20 exists between the inner ring turntable 3 and the outer ring turntable 4, and the inner ring turntable 3 and the outer ring turntable 4 can rotate relatively or oppositely.
The bearing 22 may be a rolling bearing, or a sliding bearing,
the sliding bearing can be made of copper or nonmetallic wear-resistant materials such as Polytetrafluoroethylene (PTFE) \polyphenylene sulfide (PPS) \polyoxymethylene resin (POM) \nylon 66 (PA 66), and the wear resistance of the sliding bearing is improved.
The inner ring turntable 3 is fixed on an output shaft of the first motor 7 through a coupler, the outer edge of the outer ring turntable 4 is provided with meshing teeth 16, a transmission rod is fixed on an output shaft of the second motor 14 through a connecting shaft sleeve 12, an upper meshing gear 10 and a lower meshing gear 11 are respectively fixed on the transmission rod at the heights corresponding to the upper layer sample disk and the lower layer sample disk, the meshing gears are meshed with the outer ring turntable 4, and the outer ring turntable 4 is driven to rotate through the second motor 14.
In order to reliably detect the incubator on the inner and outer ring turntables, when the inner ring turntables 3 rotate, the second motor 14 applies braking torque to the outer ring turntables 4 or starts a band-type brake function, so that the outer ring turntables 4 are ensured not to be driven to rotate by the inner ring turntables 3; when the second motor 14 drives the outer ring turntable 4 to rotate, the first motor 6 applies a braking torque to the inner ring turntable 3 or starts a band-type brake function, so that the inner ring turntable 3 is ensured not to be driven to rotate by the outer ring turntable 4.
The data acquisition unit comprises a detection support 21 and a detection mechanism arranged on the detection support, wherein the detection support 21 is arranged on the fixed base 5 in a sliding manner, and is driven by the linear motor 13 to approach or depart from the sample tray. The detection mechanism comprises two sets corresponding to the upper layer sample tray and the lower layer sample tray respectively, each set of detection mechanism comprises a laser analysis module 8 positioned above the incubator and a camera 9 positioned at the side of the incubator, and acquired data information is transmitted to a data processing unit (not shown in the figure) for storage and analysis.
The control unit (not shown in the figure) is used for coordinating and controlling the work of the culture unit and the data acquisition unit.
Two sets of proximity sensors (not shown in the figure) corresponding to the upper layer sample tray and the lower layer sample tray are further arranged on the detection support 21, and an inner ring rotary table 3 and an outer ring rotary table 4 of each layer sample tray are respectively provided with an inner ring detection blank area 15 and an outer ring detection blank area 15 for matching with the positions of the inner ring rotary table and the outer ring rotary table sensed by the proximity sensors. The proximity sensor and the blank area are used for marking the detection starting point and the detection ending point of the inner ring turntable and the outer ring turntable. For example, during detection, the outer ring turntable is detected firstly, and when the proximity sensor senses a blank area, the data acquisition device starts to acquire the data of the incubators on the outer ring turntable one by one until the proximity sensor senses the blank area of the outer ring turntable again, and the detection of the outer ring turntable is finished; and rotating the inner ring turntable until the proximity sensor senses a blank area of the inner ring turntable, moving the data acquisition unit to a detection area of the inner ring turntable by the linear motor, starting to detect the incubators on the inner ring turntable one by one, and ending the detection of the inner ring turntable until the proximity sensor senses the blank area of the inner ring turntable again.
The automatic detection system is matched with the incubator for use, and can automatically detect the flora cultured in the microorganism incubator on the premise of not opening the microorganism incubator.
As shown in fig. 4, the automatic detection system operates as follows:
the initial state is the incubator material loading process, drives inner and outer circle carousel synchronous rotation by first motor and second motor respectively, carries out inner and outer circle material loading by the manual work, and is up to the material loading completion.
And after loading is completed, starting to record time, and when the preset flora culture time (24 hours in a conventional way) is reached, starting to detect the culture device on the outer ring turntable by the detection mechanism. The detection mechanism is fixed relative to the outer ring turntable, so that the second motor drives the meshing gear to synchronously drive the upper and lower outer ring turntables to rotate, the incubators are detected one by one, and the detection mechanism reports data and image information to the data processing unit in real time for data analysis and recording.
In order to avoid that the incubator on the inner ring turntable slides to interfere with the detection and analysis of the outer ring incubator, when the outer ring turntable rotates, the inner ring turntable on the upper and lower layers is kept static under the action of the reverse moment of the first motor or the action of the band-type brake device.
When the outer ring turntable detection system detects that the outer ring turntable is finished, the outer ring turntable is reset to a blank area, the inner ring turntable is also reset to the blank area, and the linear motor drives the detection support to convey the detection mechanism to the detection position of the inner ring turntable. At this time, the detection mechanism is fixed relative to the inner ring turntable, so that the first motor directly drives the upper and lower inner ring turntable to synchronously move, the incubators are detected one by one, and the detection mechanism reports data and image information to the data processing unit in real time for data analysis and recording.
In order to avoid that the sliding of the incubator on the outer ring turntable interferes with the detection and analysis of the inner ring turntable, when the inner ring turntable rotates, the upper and lower outer ring turntables keep static under the action of the reverse moment of the second motor or the action of the band-type brake device.
After the inner ring turntable is detected, the detection support drives the detection mechanism to move outwards, the detection mechanism resets to the detection position of the outer ring turntable, and a lower detection command is waited for.
And (3) after the detection is completed for 14 days, issuing a detection completion command, and taking away the cultured flora by a worker, thereby automatically completing the culture and detection process of bacteria.
Claims (3)
1. A microbiological incubator comprising an automatic detection system; the automatic detection system comprises:
the culture unit comprises a transmission device and a sample tray for placing the incubator, the sample tray is linked with the transmission device, and the transmission device transmits the incubator on the sample tray to a collection area of the data collection unit according to a transmission instruction of the control unit; the sample tray is provided with a plurality of positioning holes for placing the incubator, and the positioning holes are arranged at least twice along the circumferential direction of the sample tray; the sample disk comprises an inner ring turntable and an outer ring turntable, and the outer ring turntable is sleeved on the outer edge of the inner ring turntable through a bearing;
the data acquisition unit is used for acquiring data of flora in the incubator in the acquisition area according to the detection instruction of the control unit; the data acquisition unit comprises: the detection bracket is linked with the linear motor; the detection mechanism is arranged on the detection bracket and is used for collecting data of flora in the incubator in the collection area; the linear motor is used for driving the detection bracket to linearly move relative to the culture unit and comprises a first motor in linkage with the inner ring turntable and a second motor in linkage with the outer ring turntable; the inner ring turntable and the outer ring turntable are respectively provided with an induction area of the proximity sensor;
the data processing unit is used for receiving and storing the data information acquired by the data acquisition unit and analyzing and processing the data information;
the timing unit is used for triggering to start timing after receiving the timing signal, and sending a timing completion signal to the control unit after the timing time length reaches the preset time length;
and the control unit is used for sending out a transmission instruction and a detection instruction according to a preset program after receiving the timing completion signal, and controlling the culture unit and the data acquisition unit to work.
2. The microbial incubator of claim 1, wherein the inner ring turntable is fixed to the output shaft of the first motor via a first coupling; the outer edge of the outer ring turntable is provided with meshing teeth, the output shaft of the second motor is fixed with a meshing gear through a second coupler, and the meshing gear is meshed with the meshing teeth of the outer ring turntable.
3. The microbial incubator of claim 1, wherein the sample tray comprises at least two layers coaxially disposed up and down and rotated in synchronization; the data acquisition unit at least comprises two sets of detection mechanisms, and the detection mechanisms are in one-to-one correspondence with the sample discs.
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| CN201710614879.2A CN107287109B (en) | 2017-07-25 | 2017-07-25 | Automatic detection system of microorganism incubator and microorganism incubator with same |
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