CN109022267B - Automatic sterile observation incubator for biological culture - Google Patents
Automatic sterile observation incubator for biological culture Download PDFInfo
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- CN109022267B CN109022267B CN201811030039.2A CN201811030039A CN109022267B CN 109022267 B CN109022267 B CN 109022267B CN 201811030039 A CN201811030039 A CN 201811030039A CN 109022267 B CN109022267 B CN 109022267B
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- 238000001514 detection method Methods 0.000 claims abstract description 57
- 238000012545 processing Methods 0.000 claims description 34
- 238000004458 analytical method Methods 0.000 claims description 15
- 239000003638 chemical reducing agent Substances 0.000 claims description 9
- 238000009434 installation Methods 0.000 claims description 6
- 239000013307 optical fiber Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 101100190621 Arabidopsis thaliana PLC4 gene Proteins 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 230000000007 visual effect Effects 0.000 description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Abstract
The invention discloses an automatic sterile observation incubator for biological culture. Is connected with a control computer through a switch; the culture bottle detection guide rail is arranged in the culture box host machine and comprises a guide rail bracket divided into an upper culture area and a lower culture area, a chain guide rail is arranged on the guide rail bracket, the chain guide rail is an E-shaped closed circulating rail, a chain wheel is arranged on the chain guide rail, the chain is arranged around the chain guide rail and the chain wheel in a closed manner, a culture bottle tray is arranged on the chain, and culture bottles are placed on the culture bottle tray; the chain moves along the chain guide rail under the drive of the chain wheel, and the upper and lower culture areas are provided with incubator sterile detection modules which comprise a cross rod, a light source, a camera, a light source controller, a laser and a detector. The invention can automatically complete the culture work of the culture bottle in a period, monitor the colony growth condition in the culture bottle in real time, and can be applied to various biological culture instruments to realize automatic monitoring and judgment of the biological culture state.
Description
Technical Field
The invention relates to an incubator for biological culture, in particular to an automatic sterile observation incubator for biological culture.
Background
The incubator is used as a common instrument in the pharmaceutical industry for biological culture and has the function of providing a constant-temperature environment for biological culture. In the biological culture or pharmaceutical process at present, the culture flask is cultivated in the incubator, the culture flask is taken out at regular time to observe the culture condition, whether colony growth is observed by naked eyes or not is detected, the influence of human factors is large, the incubator is taken out, the temperature environment in the cultivation process is destroyed, and great influence is brought to the cultivation. The incubator is driven to move by the transmission guide rail, and the visual detection and laser headspace analysis method is used for detecting whether the incubator is sterile or not, so that the incubator can be monitored in real time under a constant temperature environment, and the efficiency and reliability of biological culture are improved.
Disclosure of Invention
Aiming at the problems existing in the existing test method, the invention aims to provide an automatic sterile observation incubator for biological culture, which is used for completing constant temperature detection, automatic transmission and automatic detection of a culture bottle.
The invention is realized by the following technical scheme:
including a control computer.
Comprises a incubator host connected with a control computer through a switch.
The culture bottle detection guide rail is arranged in a host machine of the culture box, the culture bottle detection guide rail comprises a guide rail bracket divided into an upper culture area and a lower culture area, the bottom of each culture area of the guide rail bracket is a hollowed-out bottom plate, a chain guide rail is arranged on the hollowed-out bottom plate and is an E-shaped closed circulating rail, a chain wheel is arranged at one end of the chain guide rail, the chain is arranged around the chain guide rail and the chain wheel in a closed manner, a plurality of culture bottle trays are arranged on the chain, and culture bottles are placed on each culture bottle tray; the chain wheels of the upper layer culture area and the lower layer culture area of the guide rail bracket are coaxially connected through the same chain wheel shaft which vertically penetrates through the culture rail bracket, a motor connecting plate is arranged at the top of the culture bottle detection guide rail, a motor and a speed reducer are arranged on the motor connecting plate, and the motor is connected with the chain wheel shaft through the speed reducer; the motor drives the sprocket shaft to rotate, and the chain moves along the chain guide rail under the drive of the sprocket, so that each culture bottle on the chain is driven to continuously move; the upper layer culture area and the lower layer culture area of the guide rail bracket are respectively provided with an incubator sterile detection module, and the incubator sterile detection module comprises a cross rod, a light source, a camera, a light source controller, a laser and a detector; the cross rod is fixed at the top of the culture area, one side of the bottom surface of the cross rod is fixed with a camera support, the camera is fixed with a camera support, the other side of the bottom surface of the cross rod is fixed with a light source support, a detector, a light source and a station identification sensor are sequentially installed and fixed on the light source support from top to bottom, a laser support is fixed in the middle of the bottom surface of the cross rod, a laser is fixed on the laser support, the detector and the laser are located at the same installation height, and the light source and the camera are located at the same installation height; the camera and the light source are distributed on two sides of the culture flask, the laser and the detector are distributed on two sides of the culture flask to form a headspace analysis module, the station identification sensor is used for detecting that the culture flask reaches a detection position, the detector processing circuit is fixed on the guide rail support, the position is close to the detector of the sterile detection module of the culture box and is used for processing the detector to obtain a laser signal, the field of view of the camera is aligned to a solution part in the culture flask, and the laser and the detector are used for measuring the concentration change of the headspace gas in the culture flask.
The middle part of the chain guide rail of the upper culture area is provided with a plurality of through holes which are used for communicating the upper culture area and the lower culture area, and the through holes penetrate through the hollowed-out bottom plate of the upper culture area.
The bottom of the culture bottle tray is fixed on the chain through an accessory and a fastener, and the bottom of the culture bottle is embedded into the culture bottle tray.
The whole shape of the chain guide rail is E-shaped, the space is fully utilized, the turning positions of the chain guide rail are semicircular or 1/4 circle, the chain wheel is positioned at the middle extending end position of the E-shaped chain guide rail, and the chain guide rail is freely expanded according to the quantity of culture bottles.
The incubator host is provided with a temperature control panel, a lower computer PLC, a light source controller, a headspace processing controller, a compressor, a condenser and the like; the temperature control panel is connected with a temperature sensor arranged on the inner wall of the incubator host, and the temperature sensor is connected with the compressor and the condenser and is used for controlling the temperature change of the inner cavity of the incubator; the light source controller is connected with the light source, the headspace processing controller is respectively connected with the laser and the detector, the camera is directly connected to the control computer, and the light source controller and the headspace processing controller are connected to the control computer through the lower computer PLC.
The headspace processing controller sends laser to the headspace gas part of the culture flask through the laser optical fiber, the light is transmitted to the detector after being transmitted, the detector receives the laser and transmits a laser signal to the headspace processing controller, and finally the headspace processing controller is connected with the control computer through the serial port line.
The incubator host machine include chamber door, box and fuma wheel, the chamber door adopts transparent material to make, and the blake bottle detects the guide rail and arranges in the box, installs fu Ma Lun in the four corners of box bottom for the box can remove everywhere.
The sterile detection module in the incubator comprises two parts of modules, namely an image detection colony module utilizing a machine vision image recognition technology and a culture bottle top air body analysis module utilizing a coordinated laser spectrum technology. Automatic sterile observation of organisms in the culture bottle is completed through cooperation of the two part modules.
The invention utilizes the E-shaped circulating chain guide rail to drive the culture flask to culture, and can fully utilize the space in the box unlike the common chain with circular movement.
The invention utilizes the headspace gas and image of the headspace analysis culture flask to detect the liquid part, is applicable to the colony which is firstly subjected to gas change or image change, and can find whether the colony grows in the incubator earlier.
By means of the technical scheme, the automatic sterile observation incubator provided by the invention has at least the following advantages:
the invention controls the culture bottle to circularly rotate in the incubator by using the control computer, detects the colony growth condition of the liquid part of the culture bottle by using visual detection, monitors the carbon dioxide concentration of the air body at the top of the culture bottle by using headspace analysis, and judges the existence of bacteria in the culture bottle in the culture period by using the visual detection and the headspace analysis on the analysis results of the liquid and gas parts in the culture bottle.
The automatic sterile observation incubator records the time of placing the culture bottle into the incubator body by scanning the two-dimensional code on the culture bottle, records the period, and prompts operators in the form of short messages and control computer software interface information when the culture bottle period is ended or the culture bottle is detected to grow on a colony by the detection module.
The detection system and the detection mode can free operators, achieve the robot changing effect by utilizing intelligent detection and data recording means, are applicable to various biological cultures, and are easy to popularize and use.
Drawings
FIG. 1 is an automated sterile viewing incubator of the present invention.
FIG. 2 is a block diagram of an automatic sterile observation incubator according to the present invention.
Fig. 3 is a diagram of the structure of the moving guide rail of the present invention.
Fig. 4 is a block diagram of a detection module according to the present invention.
FIG. 5 is a schematic view of the internal structure of the incubator main unit of the present invention.
In the figure: 1. control computer, 2, switch, 3, incubator host computer, 4, lower computer PLC,5, light source controller, 6, headspace processing controller, 7, temperature control panel, 8, culture bottle detection guide rail, 9, chamber door, 10, box, 11, fu Ma Lun, 12, guide rail bracket, 13, motor, 14, speed reducer, 15, motor connecting plate, 16, culture bottle, 17, culture bottle tray, 18, sprocket, 19, sprocket shaft, 20, chain guide rail, 21, hollowed bottom plate, 22, incubator sterile detection module, 23, station identification sensor, 24, light source bracket, 25, detector, 26, laser bracket, 27, laser, 28, cross bar, 29, camera bracket, 30, camera, 31, light source, 32, detector processing circuit.
Description of the embodiments
The invention will be described in further detail with reference to the accompanying drawings and specific examples.
As shown in FIG. 1, the implementation of the invention comprises a control computer 1, an incubator host computer 3 and an incubator detection guide rail 8, wherein the incubator intelligent sterile detection system is arranged in the control computer 1, the incubator host computer 3 is connected with the control computer 1 through a switch 2, and the incubator detection guide rail 8 is arranged in the incubator host computer 3.
As shown in fig. 2-4, the culture bottle detection guide rail 8 comprises a guide rail bracket 12, a motor 13, a speed reducer 14, a sprocket shaft 19, a sprocket 18, a chain guide rail 20, a chain and a hollowed-out bottom plate 21; the culture bottle detection guide rail 8 comprises a guide rail bracket 12 divided into an upper layer culture area and a lower layer culture area, the bottom of each layer culture area of the guide rail bracket 12 is provided with a hollowed-out bottom plate 21, the hollowed-out bottom plate 21 is provided with a chain guide rail 20, the chain guide rail 20 is an E-shaped closed circulation rail, one end of the chain guide rail 20 is provided with a chain wheel 18, the chain is arranged around the chain guide rail 20 and the chain wheel 18 in a closed manner, the chain and the chain guide rail 20 form a sliding embedding relationship, a plurality of culture bottle trays 17 are arranged on the chain, and culture bottles 16 are placed on each culture bottle tray 17; the chain wheels 18 of the upper and lower culture areas of the guide rail bracket 12 are coaxially connected through the same chain wheel shaft 19 which is vertically and penetratingly arranged, a motor connecting plate 15 is arranged at the top of the culture bottle detection guide rail 8, a motor 13 and a speed reducer 14 are arranged on the motor connecting plate 15, and the motor 13 is connected with the chain wheel shaft 19 through the speed reducer 14; the motor 13 drives the sprocket shaft 19 to rotate, and the chain is driven by the sprocket 18 to move along the chain guide rail 20, so that each culture bottle on the chain is driven to continuously move.
As shown in fig. 3-4, the upper and lower culture areas of the guide rail bracket 12 are provided with an incubator sterility test module, which comprises a cross bar 28, a light source 31, a camera 30, a light source controller 5, a laser 27 and a detector 25; the cross bar 28 is fixed at the top of the culture area, one side of the bottom surface of the cross bar 28 is fixed with a camera support 29, a camera 30 is fixed with a camera support 29, the other side of the bottom surface of the cross bar 28 is fixed with a light source support 24, the light source support 24 is sequentially provided with a detector 25, a light source 31 and a station identification sensor 23 from top to bottom, the middle of the bottom surface of the cross bar 28 is fixed with a laser support 26, the laser support 26 is fixed with a laser 27, the detector 25 and the laser 27 are positioned at the same installation height, and the light source 31 and the camera 30 are positioned at the same installation height; the detector 25 and the laser 27 pass through the culture flask 16; the camera 30 and the light source 31 are distributed on two sides of the culture flask 16, the laser 27 and the detector 25 are distributed on two sides of the culture flask 16 to form a headspace analysis module, the station recognition sensor 23 is used for detecting that the culture flask 16 reaches a detection position, the detector processing circuit 32 is fixed on the guide rail bracket 12, the position is close to the detector 25 of the incubator sterile detection module 22 and is used for processing the detector 25 to obtain laser signals, the field of view of the camera 30 is aligned to a solution part in the culture flask 16, and the laser 27 and the detector 25 measure the concentration change of a headspace gas in the culture flask 16.
As shown in fig. 3-5, the motor 13 is connected with a sprocket shaft 19 through a speed reducer 14, and the sprocket shaft 19 drives a sprocket 18 to drive a chain to rotate; the middle part of the chain guide rail 20 of the upper culture area is provided with a plurality of through holes for communicating the upper culture area and the lower culture area, and the through holes penetrate through the hollowed-out bottom plate 21 of the upper culture area. The bottom of the culture bottle tray 17 is fixed on the chain through accessories and fasteners, and the bottom of the culture bottle 16 is embedded into the culture bottle tray 17. The whole shape of the chain guide rail 20 is E-shaped, the space is fully utilized, the turning positions of the chain guide rail 20 are semicircular or 1/4 circle, the chain wheel is positioned at the middle extending end position of the E-shaped chain guide rail 20, and the chain guide rail 20 is freely expanded according to the quantity of culture bottles; the visual detection module and the headspace analysis module for detecting the culture flask are positioned opposite to one side where the chain wheel is positioned.
The incubator sterile detection module is disposed at a detection station, which in particular is disposed at a position that is not extended from the middle of the E-shape of the chain guide 20.
As shown in fig. 2, the incubator main unit 3 is provided with a temperature control panel 7, a lower computer PLC4, a light source controller 5, a headspace processing controller 6, a compressor, a condenser, and the like; the temperature control panel 7 is connected with a temperature sensor arranged on the inner wall of the incubator host 3, and is connected with a compressor and a condenser for controlling the temperature change of the inner cavity of the incubator; the light source controller 5 is connected with the light source 22 through a cable, so that the brightness of the light source can be controlled, the headspace processing controller 6 is respectively connected with the laser 27 and the detector 25, the camera 30 is directly connected to the control computer 1, the images can be acquired in real time, and the light source controller 5 and the headspace processing controller 6 are connected to the control computer 1 through the lower computer PLC 4.
As shown in fig. 4, the headspace processing controller 6 emits laser to the headspace gas portion of the culture flask through the optical fiber of the laser 27, the light is transmitted to the detector 25 after passing through, the detector 25 receives the laser and transmits a laser signal to the detector processing circuit 32, the processed signal is transmitted to the headspace processing controller 6, and finally the headspace processing controller 6 is connected with the control computer 1 through a serial line.
Incubator host computer 3 includes chamber door 9, box 10 and fuma wheel 11, and chamber door 9 adopts transparent material to make, and blake bottle detects guide rail 8 and arranges in box 10, and fuma wheel 11 is installed in the four corners of box 10 bottom for box 10 can move everywhere.
The control computer 1 communicates with a lower computer PLC4 in the incubator host 3 by using a network cable through the switch 2, and the lower computer PLC4 controls the motor 13 to rotate according to a computer control signal.
The specific implementation working process of the invention is as follows:
the automatic sterile incubator culturing process in the implementation comprises the following steps: the culture flask 16 is put into the incubator host 3, the culture flask 16 is automatically cultured and detected in the incubator, and the culture flask detects bacteria or the culture is completed and taken out.
The culture flask 16 is put into the incubator main unit 3: before the incubator host computer 3 is ready to be placed into the incubator 16, the temperature of the incubator host computer 3 is required to be adjusted to the culture required temperature of the incubator 16, after the temperature is stable, the two-dimensional codes on the bodies of the incubator 16 are identified by the code scanning gun, then the two-dimensional codes are placed on the incubator tray 17 of the upper and lower layer culture areas in the incubator box 10, unique digital codes are marked on the incubator tray 17, after the incubator is placed, the incubator door 9 is closed, the position of the incubator tray 17 where the layer corresponding to the incubator is placed and the position of the layer corresponding to the incubator is placed is input in the intelligent sterile detection system of the control computer 1, and at the moment, the intelligent sterile detection system starts timing culture on the placed incubator.
The culture flask 16 is automatically cultured and detected in the incubator main unit 3: the culture flask 16 is cultivated at constant temperature in the incubator main unit 3, the chain guide rail 20 in the incubator main unit 3 is divided into an upper layer and a lower layer, the culture flask 16 is fixed on each layer of culture flask tray 17, and the culture flask tray 17 is fixed with a chain accessory through a fastener, so that the culture flask 16 moves on the chain guide rail 20 in the incubator main unit 3 along with the chain of the chain wheel 18 driven by the motor 13.
The culture bottle 16 moves through the detection module, the station identification sensor 23 obtains information of a culture bottle tray 17 where the culture bottle 16 is positioned and information of the culture bottle moving in place, the information is sent to the lower computer PLC4, and the lower computer PLC4 communicates with the control computer 1 through a network cable; the intelligent sterile detection system transmits a light source control command to the light source controller 5 through a network cable, the light source controller 5 controls the light source 31 to be lightened, and the system control camera 30 captures an image of a liquid part of the culture bottle and transmits the image to the control computer 1 through the network cable; the intelligent sterile detection system utilizes image processing to identify the mycelium growth condition and the liquid turbidity condition of the liquid part of the incubator to judge whether bacteria grow in the culture flask, and the culture flask 16 keeps stable and slow movement in the chain guide rail 20 in the image capturing process.
After the image detection, the culture bottle 16 runs at a constant speed in the incubator host 3 along with the chain guide rail 20, the culture bottle 16 returns to the detection position after running for one period, the second detection is performed, the station identification sensor 23 obtains information of the culture bottle tray 17 where the culture bottle 16 is located and information of the movement of the culture bottle 16 in place, the information is sent to the PLC4, the PLC4 communicates with the control computer 1 through a network cable, the intelligent sterile detection is used for judging the growth condition of colonies in the culture bottle by transmitting a headspace analysis signal obtained by the laser detector 25 through a shielding wire to the detector processing circuit 32, the detector processing circuit 32 processes the signal and then transmits the signal to the headspace processing controller 6 through the shielding wire, and the intelligent sterile detection system in the control computer 1 is used for judging the growth condition of colonies in the culture bottle after the internal circuit of the headspace processing controller 6 processes the concentration of carbon dioxide in the final headspace gas.
The culture flask 16 detects bacteria or the culture is removed: the culture bottle 16 is cultivated in the main incubator body 3 for a certain period, if colony growth is detected by image measurement and headspace analysis, the control computer 1 prompts an operator to take out the culture bottle 16 with corresponding bacteria through a short message and an interface, and after taking out, the position culture bottle 16 is set in intelligent sterile detection and taken out; at the end of one test cycle, the culture bottles 16, which are not detected with bacteria, are taken out in a unified manner, and the position of which the culture bottles 16 are taken out is set in intelligent sterile test. After removal, the culture flask 16 is placed in the next batch.
In specific implementation, the intelligent sterile detection system monitors the temperature of the incubator in real time, obtains the carbon dioxide concentration of the headspace of the incubator monitored by headspace analysis, analyzes and detects images acquired by a camera, and further analyzes and processes the headspace analysis and visual analysis detection results together to give the detection result of the incubator.
In specific implementation, the intelligent sterile detection process can record the period condition of the incubator in the box from the time of placing the incubator in the box, and the end of the incubation period or the detection of the bacteria incubator can be sent to operators in a short message mode.
Therefore, the invention can utilize the detection of the air at the top of the culture bottle and the image recognition of the liquid part, can monitor the growth process of various bacterial colonies, can obtain the judging result through the image information of the air at the top and the image information of the liquid part, can find the growth trend of bacterial colonies earlier, avoids the condition that the manual recognition can only bring single information and different detection from person to person through the observation of the liquid part, protects the temperature environment of the whole growth period of the culture bottle best, and provides a new means for high-quality culture. The invention automatically completes the culture work of the culture bottle in a period, monitors the colony growth condition in the culture bottle in real time, provides the real-time culture information of the culture bottle for staff, can be applied to various biological culture instruments, and realizes automatic monitoring and judgment of the biological culture state.
Claims (2)
1. An automatic sterile observation incubator for biological culture, characterized in that:
comprises a control computer (1);
comprises a incubator host (3) which is connected with a control computer (1) through a switch (2);
the culture bottle detecting guide rail (8) is arranged in a culture box host machine (3), the culture bottle detecting guide rail (8) comprises a guide rail bracket (12) divided into an upper culture area and a lower culture area, the bottom of each culture area of the guide rail bracket (12) is a hollowed-out bottom plate (21), a chain guide rail (20) is arranged on the hollowed-out bottom plate (21), the chain guide rail (20) is an E-shaped closed circulating rail, one end of the chain guide rail (20) is provided with a chain wheel (18), the chain is arranged around the chain guide rail (20) and the chain wheel (18) in a sealing mode, a plurality of culture bottle trays (17) are arranged on the chain, and culture bottles (16) are placed on each culture bottle tray (17); the chain wheels (18) of the upper and lower culture areas of the guide rail bracket (12) are coaxially connected through a chain wheel shaft (19) which is vertically and penetratingly arranged, a motor connecting plate (15) is arranged at the top of the culture bottle detection guide rail (8), a motor (13) and a speed reducer (14) are arranged on the motor connecting plate (15), and the motor (13) is connected with the chain wheel shaft (19) through the speed reducer (14); the motor (13) drives the sprocket shaft (19) to rotate, and the chain is driven by the sprocket (18) to move along the chain guide rail (20), so that each culture bottle on the chain is driven to continuously move;
an incubator sterile detection module (22) is arranged in the upper layer culture area and the lower layer culture area of the guide rail bracket (12), and the incubator sterile detection module (22) comprises a cross rod (28), a light source (31), a camera (30), a light source controller (5), a laser (27) and a detector (25); the cross rod (28) is fixed at the top of the culture area, one side of the bottom surface of the cross rod (28) is fixed with the camera support (29), the camera (30) is fixed with the camera support (29), the other side of the bottom surface of the cross rod (28) is fixed with the light source support (24), the light source support (24) is sequentially provided with the detector (25), the light source (31) and the station identification sensor (23) from top to bottom, the middle of the bottom surface of the cross rod (28) is fixed with the laser support (26), the laser support (26) is fixed with the laser (27), the detector (25) and the laser (27) are positioned at the same installation height, and the light source (31) and the camera (30) are positioned at the same installation height; the camera (30) and the light source (31) are distributed on two sides of the culture flask (16), the laser (27) and the detector (25) are distributed on two sides of the culture flask (16) to form a headspace analysis module, the station identification sensor (23) is used for detecting that the culture flask (16) reaches a detection position, the detector processing circuit (32) is fixed on the guide rail bracket (12) and is close to the detector (25) of the sterile detection module (22) of the culture flask, the detector (25) is used for processing the laser signal, the field of view of the camera (30) is aligned to the solution part in the culture flask (16), and the laser (27) and the detector (25) measure the concentration change of the headspace gas in the culture flask (16);
the middle part of a chain guide rail (20) of the upper culture area is provided with a plurality of through holes for communicating the upper culture area and the lower culture area, and the through holes penetrate through a hollowed-out bottom plate (21) of the upper culture area;
the bottom of the culture bottle tray (17) is fixed on a chain through an accessory and a fastener, and the bottom of the culture bottle (16) is embedded into the culture bottle tray (17);
the whole shape of the chain guide rail (20) is E-shaped, the space is fully utilized, the turning positions of the chain guide rail (20) are semicircular or 1/4 circular, the chain wheel is positioned at the middle extending end position of the E-shaped chain guide rail (20), and the chain guide rail (20) is freely expanded according to the quantity of culture bottles;
the incubator host (3) is provided with a temperature control panel (7), a lower computer PLC (4), a light source controller (5), a headspace processing controller (6), a compressor, a condenser and the like; the temperature control panel (7) is connected with a temperature sensor arranged on the inner wall of the incubator host (3) and a compressor and a condenser, and is used for controlling the temperature change of the inner cavity of the incubator; the light source controller (5) is connected with the light source (31), the headspace processing controller (6) is respectively connected with the laser (27), the detector (25) and the detector processing circuit (32), the camera (30) is directly connected to the control computer (1), and the light source controller (5) and the headspace processing controller (6) are connected to the control computer (1) through the lower computer PLC (4);
the headspace processing controller (6) emits laser to the headspace gas part of the culture flask through the optical fiber of the laser (27), the light is transmitted to the detector (25) after being transmitted, the detector (25) receives the laser and transmits a laser signal to the detector processing circuit (32), the processed signal is transmitted to the headspace processing controller (6), and finally the headspace processing controller (6) is connected with the control computer (1) through a serial port line.
2. An automated sterile viewing incubator for biological growth according to claim 1, wherein: incubator host computer (3) include chamber door (9), box (10) and fuma wheel (11), chamber door (9) adopt transparent material to make, in blake bottle detects guide rail (8) arranged in box (10), fuma wheel (11) are installed in the four corners of box (10) bottom for box (10) can remove everywhere.
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| CN112300914B (en) * | 2020-11-23 | 2023-03-24 | 济南国科医工科技发展有限公司 | A double-circuit laser detection device for bacterial culture detects |
| CN112602594A (en) * | 2020-12-18 | 2021-04-06 | 霍山县百草林石斛发展有限公司 | Dendrobium officinale seedling cultivation device and use method thereof |
| EP4036212A1 (en) * | 2021-01-27 | 2022-08-03 | Eppendorf AG | Incubator and method |
| CN113528330B (en) * | 2021-07-22 | 2024-03-29 | 山东润一智能科技有限公司 | Intelligent online monitoring device and method for bacteria on object surface |
| EP4317410A1 (en) * | 2022-08-02 | 2024-02-07 | Eppendorf SE | Incubator and method |
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