CN201695033U - Automatic germiculture instrument - Google Patents
Automatic germiculture instrument Download PDFInfo
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- CN201695033U CN201695033U CN 201020184512 CN201020184512U CN201695033U CN 201695033 U CN201695033 U CN 201695033U CN 201020184512 CN201020184512 CN 201020184512 CN 201020184512 U CN201020184512 U CN 201020184512U CN 201695033 U CN201695033 U CN 201695033U
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- biphasic
- bottle
- culture
- cultivation bottle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an automatic germiculture instrument, which comprises a sealed incubator, wherein the middle of the incubator is provided with a rotatable sample plate, the peripheral edge of the rotatable sample plate is provided with at least one culture bottle base, the culture bottle base is internally provided with a biphasic culture bottle, the upper of the rotatable sample plate is provided with a mobile device, the mobile device is provided with a manipulator which can grab up the biphasic culture bottle and can enable the biphasic culture bottle to turn over up and down, and the mobile device is also provided with a camera device which can dynamically observe a culture plate in the biphasic culture bottle. The utility model reduces the complex manual culture operating procedure, reduces the culture time, and is convenient to early find the positive sample. The utility model avoids the risk that the operating personnel is contacted with the sample to cause infection, and ensures the health of the operating personnel, so that the sample in a lab can be timely found so as to report the positive result, and the precious time for clinical treatment can be won.
Description
Technical field
The utility model relates to a kind of equipment that the blood equal samples is cultivated, specifically automatic bacteria culture instrument.
Background technology
In hospital clinical and medical research test, often need the patients'blood equal samples is carried out microbial culture, so that obtain pathogenic bacterium, further carry out drug sensitivity test to bacterium, instruct clinical application.Present automatization blood cultivation method is that blood sample is joined in the culturing bottle that liquid nutrient medium is housed, put into Special Automaticization cultivation instrument then, as bacterial growth is arranged in the sample, variations such as pressure then can take place in the culturing bottle, the information of these variations can pass to operator by instrument, is about to positive sample and notifies at once out.According to working specification, laboratory operation personnel more by hand method with the positive sample streak inoculation to culture dish, after the cultivation by for some time, could get access to bacterium from culture dish.But there is sizable drawback in this process.At first blood sample is carried out microbial culture, also will carry out manual transferred species the positive sample of cultivating with automatization blood cultivation instrument.Be that this method will be through the culturing process in two stages to the blood sample cultivation, promptly automation equipment increases the separation and Culture process of bacterium culturing process and manual streak inoculation, and two processes combine the purpose that just can reach microbial culture, get access to bacterium.And manual operations will directly contact and have certain communicable sample, causes infection easily, and health of operators is constituted a threat to.In addition, because will be through the cultivation in two stages to a sample, it is long to cultivate required time.Influence clinical treatment opportunity sometimes.Moreover manual cultivation stage is because process is loaded down with trivial details, workload big and be unfavorable for sample with to doing, this also will directly influence the quality of cultivation.
The utility model content
Technical problem to be solved in the utility model is the defective that exists at existing cultural method, a kind of automatic bacteria culture instrument is provided, it can replace automatization blood cultivation and artificial two processes of streak inoculation, make sample realize whole automatizations from collecting the acquisition bacterial colony, the cultivation in two stages is united two into one, fast and exactly finish the microbial culture process of blood sample.
The technical solution of the utility model is achieved in that it comprises the thermostat container of a sealing, it is characterized in that: a rotatable sample disk is housed in the centre of thermostat container, the circumferential edges of rotatable sample disk is provided with at least one culturing bottle seat, be provided with the biphasic cultivation bottle in the culturing bottle seat, the top of rotatable sample disk is provided with a running gear, the mechanical manipulator that can pick up the biphasic cultivation bottle and the biphasic cultivation bottle is spun upside down is housed on the running gear, also is provided with the camera head that can dynamic observe on the running gear the culture plate in the biphasic cultivation bottle.
In such scheme, described biphasic cultivation bottle is made up of first culturing room and second culturing room of mutual UNICOM, and wherein the top of first culturing room is provided with sterile closed capping; The second culturing room top also is provided with the top closure that is used to seal, and top closure links to each other with culture plate, and the culture plate both sides are perfused with solid medium, and culture plate is positioned at the middle and upper part of second culturing room, and liquid nutrient medium is equipped with in the below, inside of two culturing room.
In such scheme, described running gear comprises guidepost and the mechanical arm that can move up and down along guidepost, and mechanical manipulator and camera head are contained on the mechanical arm.
In such scheme, described mechanical manipulator is the anchor clamps of an energy clamping biphasic cultivation bottle.
In such scheme, described camera head is a pick up camera, and this pick up camera is contained on the mechanical arm, is positioned at a side of mechanical manipulator and aims at the culture plate of biphasic cultivation bottle, so that the growth conditions of Real Time Observation bacterium.
In such scheme, described thermostat container upper end is provided with overhead guard, and thermostat container inside is provided with at least one gas concentration measurement propagated sensation device, also is provided with a plurality of inlet and outlet conduits on the thermostat container housing, and gas concentration in the thermostat container is regulated in such being designed for.
In such scheme, described thermostat container inside is provided with at least one heating unit, to guarantee the temperature in the thermostat container.
In such scheme, be provided with a little magnetic patch in the described biphasic cultivation bottle, permanent magnet is equipped with in corresponding culturing bottle seat below, the other drive-motor that is equipped with of permanent magnet, this demagnetic stack is used for sample and liquid nutrient medium in the stirred vessel, sample is fully contacted, to reach good enriching effect with liquid nutrient medium.
In such scheme, described permanent magnet is a horse-shoe magnet.
Compare with existing method for cultivation of bacteria, the utlity model has following characteristics:
1) the blood equal samples directly injects the biphasic cultivation bottle, and operator need not to open the biphasic cultivation bottle cap, reduces the infected danger of operator.
2) be placed with two kinds of substratum of liquid nutrient medium and solid medium in the biphasic cultivation bottle, liquid nutrient medium is used for the bacterium that increases of sample, and solid medium is used for the inoculation and the separation and Culture of sample.
The biphasic cultivation bottle has the bacterium of increasing cultivation and two of separation and Culture are cultivated function, has optimized, integrated two processes that blood sample is cultivated, and shortens quoting the time of cultivation results, improves culture effect, the clinical practice of enhancing microbial culture.
3) because the utility model also is provided with camera head, can dynamic observe the growth conditions of bacterium on the culture plate, be convenient to discovery positive sample early, for clinical treatment is striven for the quality time.
4), culture plate can be taken out convenient bacterial colony on the picking solid medium to positive sample.
5) saved numerous and diverse manual program, made cultivation more at random, fast.
Description of drawings
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is the vertical view of Fig. 1;
Fig. 3 is the structural representation of biphasic cultivation bottle of the present utility model;
Fig. 4 is the vertical view of Fig. 3;
Fig. 5 is the structural relation stereographic map of culture plate of the present utility model and top closure.
Among the figure, 1-thermostat container, the rotatable sample disk of 2-, 3-culturing bottle seat, 4-biphasic cultivation bottle, 4.1-first culturing room, the sterile closed capping of 4.2-, 4.3-second culturing room, 4.4-top closure, 5-mechanical arm, 6-mechanical manipulator, 7-camera head, the 8-guide pillar, 9-culture plate, the little magnetic patch of 10-, 11-horse-shoe magnet, the 12-drive-motor, 13-heating unit, 14-oxygen-sensitive device, 15-nitrogen filling conduit, 16-carbonic acid gas filling conduit, 17-exhaust guide.
Embodiment
As depicted in figs. 1 and 2, the utility model comprises the thermostat container 1 of a sealing, a rotatable sample disk 2 is housed in the centre of thermostat container 1, the edge of rotatable sample disk 2 is provided with several culturing bottle seats 3, be placed with biphasic cultivation bottle 4 in the culturing bottle seat 3, the top of rotatable sample disk 2 is provided with a running gear, this running gear comprises the mechanical arm 5 that guide pillar 8 and can move along guide pillar 8 easy on and off, a mechanical manipulator 6 and a camera head 7 are housed on the mechanical arm 5, this mechanical manipulator 6 can grasp biphasic cultivation bottle 4, and biphasic cultivation bottle 4 can be spun upside down, this camera head 7 is positioned at a side of mechanical manipulator 6, and the culture plate 9 in the aligning biphasic cultivation bottle 4, can dynamic observe the culture plate in the biphasic cultivation bottle 49, when mechanical manipulator 6 is mentioned biphasic cultivation bottle 4, camera head 7 will once be made a video recording to the culture plate in the biphasic cultivation bottle 49, observe bacterial growth state on the culture plate 9.
As Fig. 3, Fig. 4 and shown in Figure 5, above-mentioned biphasic cultivation bottle 4 is made up of first culturing room 4.1 and second culturing room 4.3 of mutual UNICOM, and the top that the top of first culturing room 4.1 is provided with sterile closed capping 4.2, the first culturing room 4.1 is used to the blood sample of annotating; Second culturing room, 4.3 tops also are provided with the top closure 4.4 that is used to seal, top closure 4.4 links to each other with culture plate 9, culture plate 9 both sides are perfused with solid medium, and culture plate 9 is positioned at the middle and upper part of second culturing room 4.3, and liquid nutrient medium is equipped with in the below, inside of two culturing room.
As depicted in figs. 1 and 2, be provided with heating unit 13 in the above-mentioned thermostat container 1, be used to keep the temperature of setting in the thermostat container 1; Be provided with oxygen-sensitive device 14 in the described thermostat container 1, be used to regulate gas concentration in the thermostat container 1; Certainly, the number of heating unit 13 can be according to the needs design of practical situation, and the number of gas concentration measurement propagated sensation device and classification also can be according to the needs designs of practical situation.
As depicted in figs. 1 and 2, be provided with a little magnetic patch 10 in the above-mentioned biphasic cultivation bottle 4, horse-shoe magnet 11 be housed, the horse-shoe magnet 11 other motors 12 that drive horse-shoe magnet that are equipped with little magnetic patch 10 corresponding culturing bottle seats 3 belows; Little magnetic patch 10 in the biphasic cultivation bottle 4 is used for sample and liquid nutrient medium in the stirred vessel, sample is fully contacted, to reach good enriching effect with liquid nutrient medium.
As depicted in figs. 1 and 2,3 conduits are set in the above-mentioned thermostat container 1: nitrogen filling conduit 15, carbonic acid gas filling conduit 16 and exhaust guide 17.Each conduit connects a magnetic valve, also can add more conduits as required certainly.In actual use, can regulate oxygen, carbonic acid gas, nitrogen gas concn in the thermostat container, to satisfy the demand that sample is cultivated according to the actual needs of sample.Need under the state of anaerobic, cultivate such as the sample that has, just need annotate according to a certain percentage nitrogen and carbon dioxide, this moment, carbonic acid gas air valve and nitrogen air valve were opened, CO2 and N2 enter, vent valve is opened when reaching certain pressure in the thermostat container 1, so carry out respiration cycle, can make after the several times respiration cycle is finished and form anaerobic environment in the thermostat container 1.
The analytical instrument principle of work is as follows:
Drive-motor 12 drives horse-shoe magnet 11 and rotates, thereby the generation alternating magnetic field, the little magnetic patch 10 in the biphasic cultivation bottle 4 is arranged in alternating magnetic field, moves back and forth under the effect of alternating magnetic field, make sample and liquid nutrient medium thorough mixing, to reach good enriching effect.At the initial point that stops, mechanical manipulator 6 is positioned at biphasic cultivation bottle 4 tops, is in open configuration.Mechanical manipulator 6 bare terminal ends align with the top cover of biphasic cultivation bottle 4 in vertical direction.Starter motor, mechanical manipulator 6 descends, when dropping to the top of biphasic cultivation bottle 4, mechanical manipulator 6 clamping biphasic cultivation bottles 4 are also sling it, after sample disk 2 upper edges were left in the bottom for the treatment of biphasic cultivation bottle 4, mechanical manipulator 6 carried culturing bottle 4 and spins upside down 180 °, kept resile behind the certain hour, sample disk 2 rotates, and carries out the operation of next sample.Like this, the bacterium that grows in the liquid nutrient medium will be inoculated on the solid medium of culture plate 9 both sides and carry out separation and Culture.
After rotary movement finished, at set intervals, mechanical manipulator 6 was mentioned biphasic cultivation bottle 4 successively, and lateral camera head 7 collects on the culture plate 9 by shooting and has or not bacterial growth, to obtain the current intelligence of bacterial growth on the culture plate 9.In case finding has bacterial growth on the culture plate 9, by warning device, informs the laboratory operation personnel, so that choose the bacterium colony on the culture plate early, does further bacteriological detection, strive for clinical treatment opportunity.
The instrument control unit is used to control moving of running gear, can suitably program to control unit, and " the culturing bottle upset mode " of carrying out any kind reaches the editor of " residence time ".After the upset inoculation operation finished, mechanical manipulator 6 opened, and mechanical manipulator 6 rises along guide pillar 8 with mechanical arm 5, waits for next operation.
According to the difference of sample class, have the setting of the different upset modes and the residence time, so that the separation and Culture effect is better.
The demand of sample cultivation is a lot of clinically, and the liquid nutrient medium and the solid medium on the culture plate 9 of biphasic cultivation bottle 4 inner belows all can be according to the various different types of substratum of clinical needs can.
Claims (9)
1. automatic bacteria culture instrument, it comprises the thermostat container of a sealing, it is characterized in that: a rotatable sample disk is housed in the centre of thermostat container, the circumferential edges of rotatable sample disk is provided with at least one culturing bottle seat, be provided with the biphasic cultivation bottle in the culturing bottle seat, the top of rotatable sample disk is provided with a running gear, the mechanical manipulator that can pick up the biphasic cultivation bottle and the biphasic cultivation bottle is spun upside down is housed on the running gear, also is provided with the camera head that can dynamic observe on the running gear the culture plate in the biphasic cultivation bottle.
2. automatic bacteria culture instrument according to claim 1 is characterized in that: described biphasic cultivation bottle is made up of first culturing room and second culturing room of mutual UNICOM, and wherein the top of first culturing room is provided with sterile closed capping; The second culturing room top also is provided with the top closure that is used to seal, and top closure links to each other with culture plate, and the culture plate both sides are perfused with solid medium, and culture plate is positioned at the middle and upper part of second culturing room, and liquid nutrient medium is equipped with in the below, inside of two culturing room.
3. automatic bacteria culture instrument according to claim 1 is characterized in that: described running gear comprises guidepost and the mechanical arm that can move up and down along guidepost, and mechanical manipulator and camera head are contained on the mechanical arm.
4. according to claim 1 or 3 described automatic bacteria culture instruments, it is characterized in that: described mechanical manipulator is the anchor clamps of an energy clamping biphasic cultivation bottle.
5. according to claim 1 or 3 described automatic bacteria culture instruments, it is characterized in that: described camera head is a pick up camera, and this pick up camera is contained on the mechanical arm, is positioned at a side of mechanical manipulator and aims at the culture plate of biphasic cultivation bottle.
6. automatic bacteria culture instrument according to claim 1 is characterized in that: described thermostat container upper end is provided with overhead guard, and thermostat container inside is provided with at least one gas concentration measurement propagated sensation device, also is provided with a plurality of inlet and outlet conduits on the thermostat container housing.
7. according to claim 1 or 6 described automatic bacteria culture instruments, it is characterized in that: described thermostat container inside is provided with at least one heating unit.
8. automatic bacteria culture instrument according to claim 2 is characterized in that: be provided with a little magnetic patch in the described biphasic cultivation bottle, permanent magnet is equipped with in corresponding culturing bottle seat below, the other drive-motor that is equipped with of permanent magnet.
9. automatic bacteria culture instrument according to claim 8 is characterized in that: described permanent magnet is a horse-shoe magnet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020184512 CN201695033U (en) | 2010-04-30 | 2010-04-30 | Automatic germiculture instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020184512 CN201695033U (en) | 2010-04-30 | 2010-04-30 | Automatic germiculture instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201695033U true CN201695033U (en) | 2011-01-05 |
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ID=43396274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020184512 Expired - Lifetime CN201695033U (en) | 2010-04-30 | 2010-04-30 | Automatic germiculture instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201695033U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450497A (en) * | 2014-12-15 | 2015-03-25 | 成都红柿子科技有限公司 | High-adhesive-force culture dish gyroscope |
| CN109280622A (en) * | 2018-09-20 | 2019-01-29 | 南京至泰生物医药科技有限公司 | Automatic sterilized cell culture medium |
-
2010
- 2010-04-30 CN CN 201020184512 patent/CN201695033U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450497A (en) * | 2014-12-15 | 2015-03-25 | 成都红柿子科技有限公司 | High-adhesive-force culture dish gyroscope |
| CN109280622A (en) * | 2018-09-20 | 2019-01-29 | 南京至泰生物医药科技有限公司 | Automatic sterilized cell culture medium |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110105 |
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| CX01 | Expiry of patent term |