CN107841458A - Multilayer propelling movement type microorganism constant temperature susceptibility incubator - Google Patents

Multilayer propelling movement type microorganism constant temperature susceptibility incubator Download PDF

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Publication number
CN107841458A
CN107841458A CN201711168452.0A CN201711168452A CN107841458A CN 107841458 A CN107841458 A CN 107841458A CN 201711168452 A CN201711168452 A CN 201711168452A CN 107841458 A CN107841458 A CN 107841458A
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constant temperature
box
interior
propelling movement
conveying
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CN201711168452.0A
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CN107841458B (en
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李璐璐
郭宝中
张利娟
轩小燕
李龙
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Huanghe Science and Technology College
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14Incubators; Climatic chambers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of multilayer propelling movement type microorganism constant temperature susceptibility incubator, including insulating box and conveying box, and heater and control system, constant temperature chamber interior wall upper and lower end is provided with the main body of rotating device by bearing, rotating device is to be sequentially overlapped the fixed entirety formed from top to bottom by multi-layer annular chamber, every layer of annular chamber is again multiple separate units radially by supporting plate even partition, and each separate unit is used for the culture box for storing matching after numbering respectively;Hollow intracavitary is provided with interior drive cylinder mechanism.The body seal of conveying box is connected in the insulating box side wall of the inlet and outlet side, and outer drive cylinder mechanism and conveying mechanism and testing agency are provided with conveying box.The present invention can realize is put into and extracts operation to any culture box.

Description

Multilayer propelling movement type microorganism constant temperature susceptibility incubator
Technical field:
The present invention relates to the culture of body fluid microorganism medicine sensitive to detect equipment technology field, and in particular to a kind of multilayer propelling movement type microorganism Constant temperature susceptibility incubator.
Background technology:
During the bacillary animal epidemic of test in laboratory, the inspection of generally use ne ar, it is separately cultured, is biochemical identification, pathogenic The diagnostic methods such as experiment, Serological testing, and conventional drug sensitive test is carried out to isolated strains.The susceptibility culture of microorganism at present and The special purpose device of medical test, especially mycobacteria fast-culturing susceptibility and the micro continuous detection apparatus of identification, are mainly adopted With isoperibol, carried out in liquid nutrient solution, need repeatedly to observe in incubation, find result, while liquid culture early Liquid easily leaks in transport process of taking, and the multiple steps of whole process point could be completed, and can expend excessive manpower, thing Power resource and automaticity is low.Conventionally employed disk diffusion method or broth dilution method, the former can only qualitative detection separation disease Indigenous bacteria drug sensitivity, the latter can quantify detection medicine minimum bactericidal concentration and minimal inhibitory concentration.It is higher to there is requirement condition, grasps Make it is cumbersome, take it is longer the drawbacks of.It can just draw within general more than five days and make a definite diagnosis and drug sensitivity tests, before drug sensitivity tests come out, only It can be treated by the micro-judgment of clinical veterinarian, due to being widely present for bacterial drug resistance, when often leading to be delayed optimal treatment Between, cause epidemic situation repeatedly, so that out of control, loss is huge.
The content of the invention:
The present invention is for problem and shortage existing for current existing microculture detection device, there is provided one kind can be realized largely Culture and any position is concentrated individually to take out the multilayer propelling movement type microorganism constant temperature susceptibility incubator of detection.
Technical scheme:A kind of multilayer propelling movement type microorganism constant temperature susceptibility incubator, including insulating box and conveying box, Yi Jijia Thermal and control system, the constant temperature chamber interior wall upper and lower end are provided with the main body of rotating device by bearing, rotating device Main body is connected with rotary drive motor;The rotating device is to be sequentially overlapped fixed formed from top to bottom by multi-layer annular chamber Entirety, its middle part be middle cavity, every layer of annular chamber and be multiple separate units radially by supporting plate even partition, each Separate unit is used for the culture box for storing matching after numbering respectively;The hollow intracavitary be vertically provided with respectively inner screw and Interior slide bar, one is lying on the interior purging cylinder of hollow intracavitary and is respectively fixed with nut pipe and sliding sleeve, and nut pipe is arranged on interior spiral shell On bar, sliding sleeve is arranged on interior slide bar, and Nut pole is connected with interior stepper motor;It is provided with the interior purging cylinder for knowing The position sensor or code reader of other floor height and separate unit numbering;With the constant temperature of the side of the interior purging cylinder piston rod face Inlet and outlet are provided with case side wall.
The conveying box, its body seal is connected in the insulating box side wall of the inlet and outlet side, in conveying box shell Inside is vertically provided with external screw rod and outer slide bar respectively, and one is lying on the outer purging cylinder of hollow intracavitary and is respectively fixed with spiral shell Main pipe and sliding sleeve, nut pipe are arranged on external screw rod, and sliding sleeve is arranged on outer slide bar, and Nut pole connects with the transmission of outer stepper motor Connect;The position sensor or code reader for identifying floor height and separate unit numbering are provided with the outer purging cylinder;It is described outer Inlet and outlet described in the piston rod face of purging cylinder;
Conveying mechanism, the support of conveying mechanism and institute are provided with the region between the piston rod of outer purging cylinder and inlet and outlet Outer purging cylinder is stated to be fixed together.
Testing agency is being provided with the upside of the conveying mechanism, conveying mechanism input is provided with import, position Conveying mechanism output end is provided with outlet.The conveying mechanism entrance location is provided with coding numbering device, in the inspection Survey on mechanism rack and code reader is installed.The testing agency or coding numbering device are fixed on conveying box top inner wall, Huo Zhegu It is scheduled on the outer casing stand of outer purging cylinder.
Gear ring is provided with the downside of the main body of the rotating device during body rotation of rotating device, is fixed on insulating box bottom Gear is installed, gear engages with gear ring in the rotating shaft of the rotary drive motor in portion.
In addition, the sliding door that can be sealed also is provided with the inlet and outlet position of insulating box side wall, and driving sliding door The controllable push rod opened and closed.After by controllable push rod, all sliding doors are simultaneously closed off, the main body of rotating device can be carried out Change hastens to drive, and realizes differential centrifugation effect.An at least interlayer is provided with the middle part of the culture box, is had respectively on interlayer micro- Hole.
Beneficial effect:The present invention will cultivate the different units intracavitary of box feeding insulating box different layers first with conveying box, Mass propgation box can be accommodated using the sandwich construction of superposition, rotating device can also be passed through in incubation in insulating box Rotate the purpose realized and be separately cultured.Insulating box and conveying box cooperate and can realize being put into and extracting to any culture box Operation.Interior purging cylinder therein and outer purging cylinder can carry out dispatching culture box to the unit chamber in any room.
The present invention can be achieved to microorganism differential centrifugation effect, when cultivating latter stage needs externally output, be increased using gradual Add centrifugal speed or low speed and alternately centrifuge at a high speed, make the particle that sinking speed is different, in different centrifugal speeds and not Separated in batches with centrifugation time.Culture box further realizes the purpose of separation detection by interlayer and microcellular structure.
Brief description of the drawings
Fig. 1 is the cross-sectional view of the present invention.
Fig. 2 is A-A cross-sectional views in Fig. 1.
Label in figure:1 is insulating box, and 2 be conveying box, and 3 be rotating device, and 4 be interlayer, and 51 be interior purging cylinder, and 52 be outer Purging cylinder, 6 be nut pipe, and 71 be inner screw, and 72 be external screw rod, and 81 be interior slide bar, and 82 be outer slide bar, and 91 be interior stepper motor, 92 be outer stepper motor, and 10 be bearing, and 11 be middle cavity, and 12 be testing agency's support, and 13 be testing agency, and 14 be conveyer Structure, 15 be inlet and outlet, and 16 be upper bearing (metal), and 17 be lower bearing, and 18 be gear ring, and 19 be gear, and 20 be rotary drive motor, and 21 be branch Fagging, 22 be reinforcing plate, and 23 be entrance, and 24 be slanting baffle, and 25 be outlet, and 26 be culture box, and 27 be heater, and 28 be to slide Set.
Embodiment:
As depicted in figs. 1 and 2, multilayer propelling movement type microorganism constant temperature susceptibility incubator includes insulating box 1 and conveying box 2, Yi Jijia Thermal and control system etc..
Wherein, insulating box 1 is cylinder-like structure, and its inwall upper and lower end is installed by upper bearing (metal) 16 and lower bearing 17 respectively There is the main body of rotating device 3.Rotating device 3 is to be sequentially overlapped the fixed overall annulus post formed from top to bottom by multi-layer annular chamber Body, its middle part central core region is middle cavity.Every layer of annular chamber is divided into multiple independences by the supporting plate of radially even distribution again Unit.Each separate unit is used for the culture box 26 for storing matching, the preferably side wall in each separate unit after numbering respectively The bar code or Quick Response Code of sign are set.
The main body of rotating device 3 is connected with rotary drive motor 20.Specifically, in the body rotation of rotating device 3 When be provided with gear ring 18 on the downside of the main body of the rotating device 3, be fixed on the bottom of insulating box 1 rotary drive motor 20 turn Gear 19 is installed, gear 19 engages with gear ring 18 on axle.
Heater and sprayer unit can be arranged on the hollow intracavitary of rotating device 3, or the constant temperature outside rotating device 3 The inner side of case 1.The sensor component such as temperature sensor and humidity sensor is set in insulating box 1.Temperature sensor and humidity pass The sensor components such as sensor and the condition of culture being connected in detection insulating box 1 of control system input, according to condition of culture Situation of change, control system control heater are heated, or control sprayer unit heat mend it is wet.Or in perseverance Installation blower fan in incubator 1, realize convection current using blower fan or cold wind progress temperature adjustment is introduced by blower fan, or carried out by spraying Cooling.Control system is additionally operable to control the rotating speed of rotary drive motor 20, and then realizes the variable speed drives to rotating device 3.
Due to being provided with inlet and outlet 15 in the side wall of insulating box 1, so when needing to raise speed to rotating device 3, it is also necessary to The sliding door that can be sealed is provided with the position of inlet and outlet 15 of the side wall of insulating box 1.Each sliding door can be connected as a single entity, and be provided with The controllable push rod for driving sliding door to open and close.Make controllable push rod using control system or manually control by all sliding doors while close After closing, change just is carried out to the main body of rotating device 3 and hastens to drive, realizes differential centrifugation effect.Imitated when to microorganism differential centrifugation Fruit, when needing externally output in culture latter stage, using gradually increasing centrifugal speed or low speed and alternately centrifuging at a high speed, make to sink The different particle of reduction of speed degree, separated in batches under different centrifugal speeds and different centrifugation times.
The middle part of the culture box 26 is provided with an at least interlayer, has micropore respectively on interlayer.Cultivate box 26 by every Layer and microcellular structure further realize the purpose of separation detection.
It is the phase interworking using interior purging cylinder 51 and outer purging cylinder 52 for the important component for driving each culture box 26 to move Close.
Specifically, inner screw and interior slide bar are vertically installed respectively in hollow intracavitary, one is lying in hollow intracavitary Nut pipe and sliding sleeve are respectively fixed with interior purging cylinder 51, nut pipe is arranged on inner screw, and sliding sleeve is arranged on interior slide bar, spiral shell Female bar is connected with interior stepper motor;The position for identifying floor height and separate unit numbering is provided with the interior purging cylinder 51 Put sensor or code reader.With being provided with inlet and outlet in the side wall of insulating box 1 of the side of the interior piston rod face of purging cylinder 51 15。
The body seal of conveying box 2 is connected in the side wall of insulating box 1 of 15 sides of the inlet and outlet, in the shell of conveying box 2 Inside is vertically provided with external screw rod and outer slide bar respectively, and one is lying on the outer purging cylinder 52 of hollow intracavitary and is respectively fixed with Nut pipe and sliding sleeve, nut pipe are arranged on external screw rod, and sliding sleeve is arranged on outer slide bar, and Nut pole connects with the transmission of outer stepper motor Connect;The position sensor or code reader for identifying floor height and separate unit numbering are provided with the outer purging cylinder 52;It is described 15 are imported and exported described in the piston rod face of outer purging cylinder 52.
Insulating box 1 and the mutual cooperation of conveying box 2 can be realized is put into and extracts operation to any culture box 26.It is therein Interior purging cylinder 51 and outer purging cylinder 52 can carry out dispatching culture box 26 to the unit chamber in any room.
Meanwhile the region between the piston rod of outer purging cylinder 52 and inlet and outlet 15 is provided with conveying mechanism 14, it is defeated The support and the outer purging cylinder 52 for sending mechanism 14 are fixed together.
Also need to that the upside of conveying mechanism 14 is being provided with testing agency 13, set positioned at the input of conveying mechanism 14 Import is equipped with, the output end of conveying mechanism 14 is provided with outlet.The entrance location of conveying mechanism 14 is provided with coding Numbering device, code reader is installed on the support of testing agency 13.The testing agency 13 or coding numbering device are fixed on defeated The top inner wall of case 2 is sent, or is fixed on the outer casing stand of outer purging cylinder 52.

Claims (6)

1. a kind of multilayer propelling movement type microorganism constant temperature susceptibility incubator, including insulating box and conveying box, and heater and control System processed, it is characterized in that:The constant temperature chamber interior wall upper and lower end is provided with the main body of rotating device by bearing, rotating device Main body is connected with rotary drive motor;The rotating device is to be sequentially overlapped fixed formed from top to bottom by multi-layer annular chamber Entirety, its middle part be middle cavity, every layer of annular chamber and be multiple separate units radially by supporting plate even partition, each Separate unit is used for the culture box for storing matching after numbering respectively;The hollow intracavitary be vertically provided with respectively inner screw and Interior slide bar, one is lying on the interior purging cylinder of hollow intracavitary and is respectively fixed with nut pipe and sliding sleeve, and nut pipe is arranged on interior spiral shell On bar, sliding sleeve is arranged on interior slide bar, and Nut pole is connected with interior stepper motor;It is provided with the interior purging cylinder for knowing The position sensor or code reader of other floor height and separate unit numbering;With the constant temperature of the side of the interior purging cylinder piston rod face Inlet and outlet are provided with case side wall;
The conveying box, its body seal is connected in the insulating box side wall of the inlet and outlet side, in conveying box enclosure External screw rod and outer slide bar be vertically installed respectively, one is lying on the outer purging cylinder of hollow intracavitary and is respectively fixed with nut pipe And sliding sleeve, nut pipe are arranged on external screw rod, sliding sleeve is arranged on outer slide bar, and Nut pole is connected with outer stepper motor;Institute State the position sensor or code reader being provided with outer purging cylinder for identifying floor height and separate unit numbering;The outer purging cylinder Piston rod face described in inlet and outlet;
Conveying mechanism, the support of conveying mechanism and institute are provided with the region between the piston rod of outer purging cylinder and inlet and outlet Outer purging cylinder is stated to be fixed together;
Testing agency is being provided with the upside of the conveying mechanism, conveying mechanism input is being provided with import, positioned at defeated Mechanism output end is sent to be provided with outlet.
2. multilayer propelling movement type microorganism constant temperature susceptibility incubator according to claim 1, it is characterized in that:Positioned at the conveying Mechanism entrance location is provided with coding numbering device, and code reader is provided with testing agency's support.
3. multilayer propelling movement type microorganism constant temperature susceptibility incubator according to claim 1 or 2, it is characterized in that:The detection Mechanism or coding numbering device are fixed on conveying box top inner wall, or are fixed on the outer casing stand of outer purging cylinder.
4. multilayer propelling movement type microorganism constant temperature susceptibility incubator according to claim 1, it is characterized in that:In the rotating dress Gear ring is provided with the downside of the main body put, gear, gear are installed in the rotating shaft for the rotary drive motor for being fixed on insulating box bottom Engaged with gear ring.
5. multilayer propelling movement type microorganism constant temperature susceptibility incubator according to claim 1, it is characterized in that:Also in insulating box side The inlet and outlet position of wall is provided with the sliding door that can be sealed, and the controllable push rod that driving sliding door opens and closes.
6. multilayer propelling movement type microorganism constant temperature susceptibility incubator according to claim 1, it is characterized in that:In the culture box Middle part be provided with an at least interlayer, have micropore respectively on interlayer.
CN201711168452.0A 2017-11-21 2017-11-21 Multilayer push type microorganism constant temperature drug sensitive incubator Active CN107841458B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998370A (en) * 2018-09-18 2018-12-14 安图实验仪器(郑州)有限公司 The susceptibility automatic incubation system of microorganism
CN109856398A (en) * 2019-01-31 2019-06-07 京东方科技集团股份有限公司 Vaccine detection device and its detection method
CN110711617A (en) * 2019-10-30 2020-01-21 松塔知识产权运营武汉有限公司 Heating system for continuous inspection operation
CN114107039A (en) * 2022-01-28 2022-03-01 珠海迪尔生物工程股份有限公司 Microorganism drug sensitivity analyzer

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JP2004166554A (en) * 2002-11-19 2004-06-17 Sanyo Electric Co Ltd Incubator
US20090274348A1 (en) * 2008-04-30 2009-11-05 Ortho-Clinical Diagnostics, Inc. Immunodiagnostic test apparatus having at least one imager to provide agglutination evaluations during centrifugration cycle
CN102174395A (en) * 2011-01-30 2011-09-07 中国科学院广州生物医药与健康研究院 Automation augmentation and culture system of induced pluripotent stem cells
CN102378945A (en) * 2008-12-23 2012-03-14 佐马技术有限公司 Flexible manufacturing system

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Publication number Priority date Publication date Assignee Title
JP2004166554A (en) * 2002-11-19 2004-06-17 Sanyo Electric Co Ltd Incubator
US20090274348A1 (en) * 2008-04-30 2009-11-05 Ortho-Clinical Diagnostics, Inc. Immunodiagnostic test apparatus having at least one imager to provide agglutination evaluations during centrifugration cycle
CN102378945A (en) * 2008-12-23 2012-03-14 佐马技术有限公司 Flexible manufacturing system
US20170058508A1 (en) * 2008-12-23 2017-03-02 XOMA CORPORATION XOMA Ltd. Flexible manufacturing system
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998370A (en) * 2018-09-18 2018-12-14 安图实验仪器(郑州)有限公司 The susceptibility automatic incubation system of microorganism
CN109856398A (en) * 2019-01-31 2019-06-07 京东方科技集团股份有限公司 Vaccine detection device and its detection method
CN110711617A (en) * 2019-10-30 2020-01-21 松塔知识产权运营武汉有限公司 Heating system for continuous inspection operation
CN110711617B (en) * 2019-10-30 2020-09-01 浙江达普生物科技有限公司 Heating system for continuous inspection operation
CN114107039A (en) * 2022-01-28 2022-03-01 珠海迪尔生物工程股份有限公司 Microorganism drug sensitivity analyzer

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