CN109856398A - Vaccine detection device and its detection method - Google Patents
Vaccine detection device and its detection method Download PDFInfo
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- CN109856398A CN109856398A CN201910100705.3A CN201910100705A CN109856398A CN 109856398 A CN109856398 A CN 109856398A CN 201910100705 A CN201910100705 A CN 201910100705A CN 109856398 A CN109856398 A CN 109856398A
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Abstract
The application provides a kind of vaccine detection device and its detection method.The vaccine detection device is used to detect the validity of vaccine, the vaccine is the simply connected vaccine of inactivation type or attenuation type, vaccine detection device includes: main body, sense channel is equipped in main body, sense channel includes drawing end and driving end, and sense channel is successively arranged conversion zone and detection zone by drawing end to driving extreme direction, conversion zone is equipped with first antibody, in conjunction with the pathogen of the inactivation type in vaccine to be detected or attenuation type antigen and antibody specific can occur for first antibody, and the first antibody of conversion zone is through colloid gold label, detection zone is equipped with first antibody；Driving assembly, driving assembly are located at the driving end of sense channel, and for driving vaccine to enter sense channel by the end of drawing of sense channel, and flow by driving direction.The detection method of the vaccine is detected using the vaccine detection device.The vaccine detection device of the application can quickly detect the validity of vaccine.
Description
Technical field
This application involves a kind of vaccine detection device and its detection methods.
Background technique
Inactivation type or attenuation type simply connected vaccine are by making pathogen (virus or bacterium) lose activity or subtract to the mankind
Murder by poisoning type be prepared.This kind of vaccine remains the antigenic characteristic of pathogen, can cause the specific immune response of body, make
The mankind obtain the immunoprotection to the pathogen.Such vaccine preservation condition is harsh, it usually needs be protected from light under cryogenic conditions
It saves, is easy to fail in transport and storing process.Domestic simultaneously also to report a large amount of vaccine fraud events, this just makes people very
Hardly possible judges whether the vaccine of inoculation is effective.
The validation checking of detection vaccine still relies on large-scale testing agency and carries out at present.This detection method time-consuming consumption
Power.And there are many single branch amount of vaccine expended when detecting, and the dosage of general vaccine is few, has been unable to reach epidemic disease after tested
Effective dosage of inoculation of seedling.Therefore vaccine detection at present can only rest on the sampling observation stage, whether can not detect vaccine before inoculation
Effectively.
Summary of the invention
The present invention, which provides a kind of vaccine detection device and its detection method, the vaccine detection device, quickly to be had vaccine
Effect property is detected.
To achieve the above object, the embodiment of the present invention provides a kind of vaccine detection device.The vaccine detection device is for examining
The validity of vaccine is surveyed, the vaccine is the simply connected vaccine of inactivation type or attenuation type, and the vaccine detection device includes:
Main body, the main body is interior to be equipped with sense channel, and the sense channel is including drawing end and driving end, and the detection
Channel is successively arranged conversion zone and detection zone by end to the driving extreme direction that draws, and the conversion zone is equipped with the
With the pathogen of the inactivation type in the vaccine to be detected or attenuation type antigen-antibody can occur for one antibody, the first antibody
Specific binding, and the first antibody of the conversion zone is through colloid gold label, the detection zone are equipped with described the
One antibody；
Driving assembly, the driving component are located at the driving end of the sense channel, and for driving the vaccine by institute
The end of drawing for stating sense channel enters the sense channel, and flows by driving direction.
Optionally, the first antibody through the colloid gold label of the conversion zone is by way of Electrostatic Absorption
Point sample is on the tube wall of the sense channel；
And/or the first antibody of the detection zone passes through investment point sample on the tube wall of the sense channel.
Optionally, the driving component includes the piston and piston rod being connected, and it is logical that the piston is arranged in the detection
The driving end in road, and the axial reciprocating for capableing of the sense channel described in the drive lower edge of the piston rod is mobile.
Optionally, the vaccine detection device further includes microfluidic components, and the microfluidic components are opposite with the main body
Fixed setting, the microfluidic components are used to limit the driving component along the axial moving distance of the sense channel.
Optionally, the microfluidic components include tune nut and with it is described tune nut thread cooperation tune screw rod,
Described to tune nut fixed setting opposite with the main body, the screw rod that tunes is connect with the driving component, and described in drive
Driving assembly is moved along the axial reciprocating of the sense channel.
Optionally, the vaccine detection device further includes drawing component, and the component that draws is set on the sense channel
End is drawn, the component that draws is syringe needle, and the syringe needle is fixed on fixing seat, and the fixing seat is connect with the main body, described
Syringe needle is connected to the end of drawing of the sense channel.
Optionally, control zone is additionally provided in the sense channel, it is separate that the control zone is located at the detection zone
One end of the conversion zone, the control zone are equipped with secondary antibody, and the secondary antibody can occur with the first antibody
Specific binding.
Optionally, the secondary antibody of the control zone is by investment point sample in the tube wall of the sense channel
On.
Optionally, the inner surface of the tube wall of the sense channel is in addition to the conversion zone, the detection zone, Yi Jisuo
It states the region outside control zone and passes through surface hydrophobicity processing.
The present embodiment also provides a kind of detection method of vaccine, and the detection method of the vaccine uses the vaccine detection device
It is detected, the detection method of the vaccine includes:
The vaccine to be detected is set to enter the detection by the end of drawing of the sense channel by the driving component
Channel, and the conversion zone and the detection zone are passed sequentially through by the driving direction；
Wherein, when the pathogen of inactivation type or attenuation type in the vaccine to be detected can be with the conversion zone
The first antibody through the colloid gold label occurs antigen and antibody specific and combines, and described the with the detection zone
One antibody reacts, to make the detection zone display color by the colloidal gold, then the vaccine to be detected
For effective vaccine；When inactivation type or attenuation type in the vaccine to be detected pathogen can not with through the colloidal gold mark
The first antibody of note reacts, then the detection zone not display color, then the vaccine to be detected is invalid epidemic disease
Seedling.
Optionally, control zone is additionally provided in the sense channel, it is separate that the control zone is located at the detection zone
One end of the conversion zone, the control zone are equipped with secondary antibody, and the secondary antibody can occur with the first antibody
Specific binding；
The detection method of the vaccine further include: when the first antibody of the colloid gold label can be compareed with described
The secondary antibody in region reacts, to make the control zone display color by the colloidal gold, then this
Detection is effective；The first antibody of the colloid gold label can not occur anti-with the secondary antibody of the control zone
It answers, then the control zone not display color, then this detection is invalid.
In the vaccine detection device of above-described embodiment and the detection method of vaccine, by the entirety that vaccine detection device is arranged
Structure can quickly detect the validity of vaccine.
Detailed description of the invention
Fig. 1 is the topology view of an exemplary embodiment vaccine detection device.
Fig. 2 is the internal structure view of an exemplary embodiment vaccine detection device.
Description of symbols
Main body 10
Sense channel 11
Draw end 111
Drive end 112
Conversion zone 113
Detection zone 114
Control zone 115
Graduation mark 116
Driving assembly 20
Piston 21
Piston rod 22
Microfluidic components 30
Tune nut 31
Tune screw rod 32
Syringe needle 40
Fixing seat 50
Bottom cover 60
Elastic component 70
Specific embodiment
Example embodiments are described in detail here, and the example is illustrated in the accompanying drawings.Following description is related to
When attached drawing, unless otherwise indicated, the same numbers in different drawings indicate the same or similar elements.Following exemplary embodiment
Described in embodiment do not represent all embodiments consistent with the application.On the contrary, they be only with it is such as appended
The example of the consistent device of some aspects be described in detail in claims, the application.
It is only to be not intended to be limiting the application merely for for the purpose of describing particular embodiments in term used in this application.
Unless otherwise defined, technical term or scientific term used in this application, which are should be in fields of the present invention, has general skill
The ordinary meaning that the personage of energy is understood.The classes such as "one" or " one " used in present specification and claims
It does not indicate that quantity limits like word yet, but indicates that there are at least one.The similar word such as " comprising " or "comprising" means
Element or object before present " comprising " or "comprising" cover the member for appearing in " comprising " or "comprising" presented hereinafter
Part or object and its equivalent, it is not excluded that other elements or object.The similar word such as " connection " or " connected " is not
It is defined in physics or mechanical connection, and may include electrical connection, it is either direct or indirect.It is " more
It is a " it include two, it is equivalent at least two.In present specification and singular used in the attached claims
"an", " described " and "the" are also intended to including most forms, unless the context clearly indicates other meaning.It should also manage
Solution, term "and/or" used herein refers to and includes one or more associated any or all of project listed can
It can combination.
Understood incorporated by reference to Fig. 1 and Fig. 2, the implementation case provides a kind of vaccine detection device.The vaccine detection device
For detecting the validity of vaccine, the vaccine is the simply connected vaccine of inactivation type or attenuation type, and the vaccine detection device includes
Main body 10 and driving assembly 20.
Sense channel 11 is equipped in main body 10, sense channel 11 is including drawing end 111 and driving end 112, and sense channel
11 are successively arranged conversion zone 113 and detection zone 114 by drawing end 111 to driving 112 direction of end.The width of sense channel 11
It is 0.3-2 millimeters, cross sectional shape can be the other shapes such as rectangle or circle.The end 111 of drawing of sense channel 11 is equipped with quarter
Line 116 is spent, graduation mark 116 is marked with 1-5 microlitres of scale.
Conversion zone 113 be equipped with first antibody, the first antibody can in the vaccine to be detected inactivation type or
The pathogen of attenuation type occurs antigen and antibody specific and combines, and the first antibody of conversion zone 113 is through colloidal gold mark
Note.Detection zone 114 is equipped with the first antibody.In the present embodiment, the institute through the colloid gold label of conversion zone 113
State first antibody by way of Electrostatic Absorption point sample on the tube wall of sense channel 11；Described the first of detection zone 114 is anti-
Body is by investment point sample on the tube wall of sense channel 11.Point sample is through the colloid gold label by way of Electrostatic Absorption
The first antibody can be such that the first antibody through the colloid gold label is relatively easily detached from from conversion zone 113, and
Shift to detection zone 114.By first antibody described in investment point sample, the first antibody is enable tively firm to be fixed on
Detection zone 114.Using investment realize immunoglobulin be fixed on substrate with point sample under stable, low flow velocity enzyme without departing from
The features such as, while being the region that antigen-antibody leaves reaction, and testing result is generated in detection zone 114.Specifically, in this reality
Apply in example, be illustrated using the first antibody in PDDA fixed test region 114, concrete operations are as follows: by spotted area into
So that it is grafted upper acrylic acid with propylene acid soak after row ultraviolet light, while the first antibody of point sample being mixed with PDDA
Point sample to acrylic acid impregnates region afterwards, and PDDA is attached together with acrylic acid, while the reticular structure of PDDA resists described first
Body wraps up wherein, and after nitrogen wind air-dries, point sample is completed.Wherein PDDA is the English letter of polydimethyl diallyl ammonium chloride
It writes.
In order to facilitate observation, the material of the tube wall of sense channel 11 is transparent material.And main body 10 is trigone structure, thus
The testing result of sense channel 11 can clearly be observed.
Driving assembly 20 is located at the driving end 112 of sense channel 11, and for driving the vaccine by sense channel 11
It draws end 111 and enters sense channel 11, and flowed by driving direction.In this way, can be driven by driving assembly 20 to be detected
The vaccine rapidly flows in sense channel 11 according to driving direction, so as to quickly complete detection；Meanwhile passing through
The mode of first antibody described in the colloid gold label, can be by direct visual perception testing result, without by it
His viewing tool, is quickly and easily detected so as to realize.
Driving assembly 20 includes the piston 21 and piston rod 22 being connected, and piston 21 is arranged in the driving end of sense channel 11
112, and can the axial reciprocating under the drive of piston rod 22 along sense channel 11 move.Piston rod 22 is driven to make by external force
Piston 21 moves back and forth, and the liquid for 11 the inside of sense channel provides driving force.
The vaccine detection device further includes microfluidic components 30, and microfluidic components 30 and main body 10 are fixed relatively to be arranged,
And it is located at the side at driving end 112 of the driving assembly 20 far from sense channel 11, microfluidic components 30 are for limiting driving assembly
20 along sense channel 11 axial moving distance, so as to finely tune liquid absorption amount, and epidemic disease to be detected needed for reaching control
Purpose of the dosage of seedling in the range of very little.The dosage of vaccine to be detected needed for the present embodiment is few, it is only necessary to 2-5 microlitres
Vaccine.Specifically, the vaccine to be detected of the present embodiment is hydrophobia, and usually every hydrophobia is (multiple
After molten) it is 0.5 milliliter, hydrophobia to be detected only accounts for the 0.4%-1% of whole branch hydrophobia total amount, will not be right
Hydrophobia inoculation has an impact.And compared with the existing technology in pass through Test paper detect hydrophobia, detection
Test paper needs more detection limit, and the dosage of hydrophobia is minimum (there was only 0.5ml mostly), and discomfort shares Test paper
Detection.
Microfluidic components 30 include tuning nut 31 and tuning screw rod 32 with tune that nut 31 is threadedly engaged, tune nut
31 fix setting with main body 10 relatively, tune screw rod 32 and connect with driving assembly 20, and drive driving assembly 20 along sense channel
11 axial reciprocating is mobile.When detection can turn tune nut 31 and drive tune screw rod 32 along the axial of sense channel 11
Subtle movement, and realize that piston 21 is moved along the subtle of axial direction of sense channel 11, thus to be detected needed for limitation
The dosage of vaccine is to a very small extent.
Further, bottom cover 60 is equipped with far from one end of driving assembly 20 tuning nut 31, bottom cover 60 and tune screw rod
Be equipped with elastic component 70 between 32, the both ends of elastic component 70 respectively with bottom cover 60 and tune screw rod 32 and connect, pass through setting elastic component
70 can be always to the application of screw rod 32 one active force for drawing end 111 towards sense channel 11 be tuned, to revolve in rotation
When adjusting screw rod 32 mobile to the direction for drawing end 111 far from sense channel 11, resistance can be increased, so as to preferably control
System enters the dosage of the vaccine to be detected for drawing end 111 of sense channel 11.
The vaccine detection device further includes drawing component, described to draw component and draw end 111 set on sense channel 11,
The component that draws is syringe needle 40, and syringe needle 40 is fixed on fixing seat 50, and fixing seat 50 is connect with main body 10, and syringe needle 40 and detection are logical
Draw 111 connection of end in road 11.It can be easily by the vaccine to be detected from ampoule in this way, drawing component by setting
It takes out, and be directly entered sense channel 11 draws end 111.
Control zone 115 is additionally provided in sense channel 11, control zone 115 is located at detection zone 114 far from conversion zone
113 one end, control zone 115 are equipped with secondary antibody, and the secondary antibody can be specifically bound with the first antibody.
The secondary antibody of control zone 115 is by investment point sample on the tube wall of sense channel 11.By the way that control zone is arranged
Whether 115 first antibodies that are able to detect in conversion zone 113 and detection zone 114 are effective, so that it is guaranteed that detection has
Effect property.
The inner surface of the tube wall of sense channel 11 is other than conversion zone 113, detection zone 114 and control zone 115
Region pass through surface hydrophobicity processing.In this way, liquid is in addition to conversion zone 113, detection zone 114 and control zone
115 region can relatively rapid move, to further shorten the time of detection.
The vaccine detection device of the present embodiment can detect the validity of vaccine before vaccine inoculation, judge to be connect
Kind whether vaccine no longer valid or false vaccine.The vaccine detection device of the present embodiment is easy to operate, the time required to detection
Short (in 5 minutes), testing result easily determines, the personnel for not needing profession operate and valuable instrument and equipment.
Specifically, when the vaccine detection device of the present embodiment is used to detect the validity of hydrophobia, dog IgG is used
As first antibody, uses one of rabbit-anti RV IgG, goat-anti RV IgG and the anti-RV IgG of mouse as secondary antibody, make herein
Use rabbit-anti RV IgG as secondary antibody.Wherein, IgG is the abbreviation of immunoglobulin G；The full name in English of RV is Rabies
Virus, Chinese are rabies viruses.Dog IgG refers to dog immunoglobulin G；Rabbit-anti RV IgG refers to that the immune system of rabbit is connecing
A kind of immunoglobulin generated when kind to hydrophobin immunoglobulin G；Goat-anti RV IgG refers to that the immune system of sheep exists
A kind of immunoglobulin generated when being inoculated into hydrophobin immunoglobulin G；The anti-RV IgG of mouse refers to the immune system of mouse
A kind of immunoglobulin generated when being inoculated into hydrophobin immunoglobulin G.
The present embodiment also provides a kind of detection method of vaccine, and the detection method of the vaccine uses the vaccine detection device
It is detected, the detection method of the vaccine includes:
The vaccine to be detected is set to enter sense channel by the end 111 of drawing of sense channel 11 by driving assembly 20
11, and conversion zone 113, detection zone 114 and control zone 115 are passed sequentially through by the driving direction；
Wherein, when the pathogen of inactivation type or attenuation type in the vaccine to be detected can be with conversion zone 113
The first antibody through the colloid gold label occurs antigen and antibody specific combination and forms pathogen-first antibody-colloid
Gold, and react with the first antibody of detection zone 114 and be capable of forming first antibody-pathogen-first antibody-colloid
Gold, to make 114 display color of detection zone by the colloidal gold, then the vaccine to be detected is effective vaccine；When
The pathogen of inactivation type or attenuation type in the vaccine to be detected can not be with described first through the colloid gold label
Antibody reacts, then the not display color of detection zone 114, then the vaccine to be detected is invalid vaccine；
When the first antibody of the colloid gold label can occur instead with the secondary antibody of control zone 115
It answers, to make 115 display color of control zone by the colloidal gold, then this detection is effective；The colloid gold label
The first antibody can not react with the secondary antibody of control zone 115, then control zone 115 does not show face
Color, then this detection is invalid.
Specifically, when the detection method of the vaccine of the present embodiment is used to detect the validity of hydrophobia, by this reality
It applies in hydrophobia (redissolution) liquid being inoculated with needed for a syringe needle for vaccine detection device 40 is inserted into, adjusting tunes nut
31, driving piston 21 is drawn syringe needle 40 after the hydrophobias to be detected (abbreviation sample herein) of 3 microlitres of amounts from vaccine
Middle taking-up.Continue to adjust and tune nut 31, makes the samples residence drawn the position of conversion zone 113 2 minutes.It adjusts and tunes again
Nut 31, so that sample is moved respectively to detection zone 114 and the position of control zone 115 respectively stops one minute.It is last reversed
Nut 31 is tuned, sample is made to remove sense channel 11.
When detecting time vaccines, detection zone 114 and 115 color of control zone are observed, if detection zone 114 is shown as red
Color, then sample is positive sample, illustrates that hydrophobia is effective vaccine.Inactivated rabies virus in positive sample is reacting
Virus-dog IgG- colloidal gold is formed in conjunction with colloid gold label dog IgG generation antigen and antibody specific at region 113.Work as inspection
Sample is moved at detection zone 114, fixed dog IgG secondary response again in virus-dog IgG- colloidal gold and detection zone 114
Dog IgG- virus-dog IgG- colloidal gold is formed, so that detection zone 114 is displayed in red.
If the display of detection zone 114 is not displayed in red, sample is negative sample, illustrates that hydrophobia is invalid epidemic disease
Seedling.
Later, sample connects the position for being moved to control zone 115, and colloid gold label dog IgG and rabbit-anti RVIgG occur anti-
Rabbit-anti RV IgG- dog IgG- colloidal gold, which should be formed, is displayed in red control zone 115, illustrates that this detects the colloidal gold used
Mark dog IgG effective, i.e., this testing result is effective.If control zone 115 is not displayed in red, testing result is invalid.
If display color, sample are not negative sample to detection zone 114, illustrate that hydrophobia is invalid vaccine.
This is because fail without inactivated rabies virus or rabies virus antigen albuminous degeneration, thus can not be in conversion zone 113 and detection
The dog IgG in region 114 reacts, and detection zone 114 is not displayed in red.
It, can be quickly to vaccine by the way that the overall structure of vaccine detection device is arranged in vaccine detection device of the invention
Validity detected.Specifically, following effective effect that the vaccine detection device of the present embodiment reaches:
1, the dosage detected is few, and present invention combination microflow control technique makes sample size only need 2-5 microlitres of vaccine, do not influence
The subsequent use of vaccine.
2, short the time required to detection, the present invention uses colloid gold immune reaction technology, and the specific binding of antibody antigen is only
Several minutes of progress are needed, the timeliness of detection has been kept.
3, do not need professional's operation, the present invention by the colour developing of observing colloid gold, judge it is effective when vaccine, simply
It is easy.
The foregoing is merely the preferred embodiments of the application, not to limit the application, all essences in the application
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should be included within the scope of the application protection.
Claims (11)
1. a kind of vaccine detection device, which is characterized in that the vaccine detection device is used to detect the validity of vaccine, the epidemic disease
Seedling is the simply connected vaccine of inactivation type or attenuation type, and the vaccine detection device includes:
Main body, the main body is interior to be equipped with sense channel, and the sense channel is including drawing end and driving end, and the sense channel
Conversion zone and detection zone are successively arranged by end to the driving extreme direction that draws, it is anti-that the conversion zone is equipped with first
Body, it is special that with the pathogen of the inactivation type in the vaccine to be detected or attenuation type antigen-antibody can occur for the first antibody
Property combine, and the first antibody of the conversion zone, through colloid gold label, it is anti-that the detection zone is equipped with described first
Body；
Driving assembly, the driving component are located at the driving end of the sense channel, and for driving the vaccine by the inspection
The end of drawing for surveying channel enters the sense channel, and flows by driving direction.
2. vaccine detection device as described in claim 1, which is characterized in that the conversion zone through the colloid gold label
The first antibody by way of Electrostatic Absorption point sample on the tube wall of the sense channel；
And/or the first antibody of the detection zone passes through investment point sample on the tube wall of the sense channel.
3. vaccine detection device as described in claim 1, which is characterized in that the driving component include the piston that is connected and
Piston rod, the piston is arranged in the driving end of the sense channel, and can examine described in the drive lower edge of the piston rod
The axial reciprocating for surveying channel is mobile.
4. vaccine detection device as described in claim 1, which is characterized in that the vaccine detection device further includes micro-fluidic group
Part, the microfluidic components fixed setting opposite with the main body, the microfluidic components are for limiting the driving component edge
The axial moving distance of the sense channel.
5. vaccine detection device as claimed in claim 4, which is characterized in that the microfluidic components include tune nut and with
It is described tune nut thread cooperation tune screw rod, it is described tune nut it is opposite with the main body it is fixed be arranged, it is described to tune spiral shell
Bar is connect with the driving component, and the driving component is driven to move along the axial reciprocating of the sense channel.
6. vaccine detection device as described in claim 1, which is characterized in that the vaccine detection device further includes the portion of drawing
Part, it is described to draw component and draw end set on the sense channel；The component that draws is syringe needle, and the syringe needle is fixed on fixation
Seat, the fixing seat are connect with the main body, and the syringe needle is connected to the end of drawing of the sense channel.
7. the vaccine detection device as described in any one of claim 1-6, which is characterized in that also set in the sense channel
There is control zone, the control zone is located at the one end of the detection zone far from the conversion zone, and the control zone is set
There is secondary antibody, the secondary antibody can be specifically bound with the first antibody.
8. vaccine detection device as claimed in claim 7, which is characterized in that the secondary antibody of the control zone passes through
Investment point sample is on the tube wall of the sense channel.
9. vaccine detection device as claimed in claim 7, which is characterized in that the inner surface of the tube wall of the sense channel in addition to
Surface hydrophobicity processing is passed through in region outside the conversion zone, the detection zone and the control zone.
10. a kind of detection method of vaccine, which is characterized in that the detection method of the vaccine is using any in claim 1-6
Vaccine detection device described in one is detected, and the detection method of the vaccine includes:
The vaccine to be detected is set to enter the sense channel by the end of drawing of the sense channel by the driving component,
And the conversion zone and the detection zone are passed sequentially through by the driving direction；
Wherein, when the pathogen of inactivation type or attenuation type in the vaccine to be detected can be with the conversion zone through institute
The first antibody for stating colloid gold label occurs antigen and antibody specific and combines, and anti-with described the first of the detection zone
Body reacts, to make the detection zone display color by the colloidal gold, then the vaccine to be detected is to have
Imitate vaccine；When inactivation type or attenuation type in the vaccine to be detected pathogen can not with through the colloid gold label
The first antibody reacts, then the detection zone not display color, then the vaccine to be detected is invalid vaccine.
11. the detection method of vaccine as claimed in claim 10, which is characterized in that be additionally provided with check plot in the sense channel
Domain, the control zone are located at the one end of the detection zone far from the conversion zone, and it is anti-that the control zone is equipped with second
Body, the secondary antibody can be specifically bound with the first antibody；
The detection method of the vaccine further include: when the first antibody of the colloid gold label can be with the control zone
The secondary antibody react, to make the control zone display color by the colloidal gold, then this is detected
Effectively；The first antibody of the colloid gold label can not react with the secondary antibody of the control zone,
The then control zone not display color, then this detection is invalid.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110275020A (en) * | 2019-08-01 | 2019-09-24 | 郑州迈迪迅医疗科技有限公司 | A kind of rotary-type chromatography detection device |
| CN113030465A (en) * | 2021-03-03 | 2021-06-25 | 广州洛民塑料有限公司 | Full-automatic vaccine censorship device |
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