CN108991228A - A kind of method and its application of the compound organic trace element of biosynthesis - Google Patents
A kind of method and its application of the compound organic trace element of biosynthesis Download PDFInfo
- Publication number
- CN108991228A CN108991228A CN201810870245.8A CN201810870245A CN108991228A CN 108991228 A CN108991228 A CN 108991228A CN 201810870245 A CN201810870245 A CN 201810870245A CN 108991228 A CN108991228 A CN 108991228A
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- China
- Prior art keywords
- bacterium solution
- trace element
- organic trace
- compound organic
- microelement
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- 235000013619 trace mineral Nutrition 0.000 title claims abstract description 78
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
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Abstract
The invention discloses a kind of method and its application of the compound organic trace element of biosynthesis, comprising steps of (1) prepares each single bacterium bacterium solution respectively；(2) mixed bacteria liquid is made in each bacterium solution obtained in (1)；(3) composite inorganic microelement material is added in carrier material and fermentation substrate is made；(4) mixed bacteria liquid and fermentation substrate are mixed to prepare to fermentation material；(5) it will pack and be sealed by fermentation to fermentation material, and obtain compound organic trace element material.Compound organic trace element material application method produced by the present invention is to be added to compound organic trace element material in the conventional diet feed for be not added with microelement according to the weight adding proportion of 2.5%-5% mixed feed is made.Compound organic trace element produced by the present invention can improve the absorption rate of microelement, reduce additive amount of the microelement in feed, reduce the pollution that microelement discharge causes soil, can improve the production performance of animal, reduce aquaculture cost.
Description
Technical field
The invention belongs to field of biotechnology and Feed Manufacturing field, and in particular to a kind of biosynthesis is compound organic micro-
The method and its application of secondary element.
Background technique
Microelement is the nutriment that must be added in livestock and poultry animal feed, can directly or indirectly participate in livestock and poultry animal
The almost all of physiological and biochemical activity of body for enhancing development, maintains metabolism, adjusting body's immunity and changes
Kind product quality etc. plays a significant role.All the time, microelement is all shape with inorganic mineral in animal and fowl fodder
Formula addition, test prove: (1) microelement-supplementing, the absorption rate of microelement in such a way that inorganic mineral adds
It is low, it is usually no more than 20%, is largely discharged so as to cause microelement, Heavy-Metal-Contaminated Environments are caused；(2) palatability is poor, due to
Metallic ore substance has bitter taste or unique smell, influences the feed intake of livestock and poultry；(3) unstability, inorganic mineral enter
Digestive system forms the free state of metal ion, easily forms the insoluble matter of stable structure in conjunction with oxalic acid, phytic acid, to cause
Microelement loses vigor；(4) metal ion is easily chemically reacted with vitamin, metabolic enzyme, to make vitamin, metabolic enzyme
It loses activity, reduces its effect.
Organic trace element is that the organic compounds such as metal ion and protein, small peptide, amino acid, organic acid, polysaccharide are logical
It crosses covalent bond or ionic bond combines the chelate or complex compound for forming stable structure, in practice it has proved that: organic trace element can improve
The advantages that production performance of livestock and poultry, raising meat, but currently, the synthesis of organic trace element mainly passes through physical chemistry side
Method is made, and needs multi-step process, and need to control different pH in links, complicated for operation, the waste of generation is more, right
Environment pollutes, while the organic trace element as made from physico-chemical process is generally that single or two kinds organic micro
The compound of organic trace elements can not be made in element simultaneously, and therefore, feeding value is single and at high cost.
Currently, microbe additive or fermented feed are widely used in livestock and poultry cultivation industry, microorganism is utilized
Biological characteristics various inorganic microelements are converted to organic trace element during microbial growth, simplify
Production technology reduces cost, while not generating any waste, not only just there is good market prospects, moreover it is possible to promote green poultry
The development of animal husbandry.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, and it is an object of the present invention to provide a kind of numerous in growth using beneficial microbe
The method that inorganic microelement is synthesized into organic trace element during growing.Under field conditions (factors), it is grown by microorganism numerous
It grows, generates metabolic enzyme, small peptide and amino acid are dissociated the feedstock into, while generating organic acid, by microorganism cofactors, by nothing
Machine is micro to be complexed or is chelated with small peptide, amino acid and organic acid etc., compound organic trace element is formed, in this low energy
The synthetic ratio that compound organic trace element obtained is produced under consumption, simple machine is up to 85% or more, with physical chemistry synthetic method
It is more more energy efficient, more convenient, while any waste will not be generated, any pollution will not be caused to environment.It makes through the invention
The compound organic trace element obtained can improve the absorption rate of microelement, reduce addition of the microelement in feed
Amount reduces microelement discharge and causes the pollution of soil, while can improve the production performance of animal, reduces aquaculture cost.
Another object of the present invention is to provide the application methods of the compound organic trace element of the biosynthesis.
For achieving the above object, the technical scheme adopted by the invention is as follows:
A kind of method of the compound organic trace element of biosynthesis, which comprises the following steps:
Step (1): bacillus subtilis, bafillus natto, sticky red rhodotorula, enterococcus faecium and Pediococcus acidilactici are connect respectively
Cultivated in independent culture medium kind after sterilizing, obtain respectively individual bacillus subtilis bacterium solution, bafillus natto bacterium solution,
Sticky red rhodotorula bacterium solution, enterococcus faecium bacterium solution and Pediococcus acidilactici bacterium solution；
Step (2): by the bacillus subtilis bacterium solution being prepared in step (1), bafillus natto bacterium solution, red ferment is glued
Female bacterium bacterium solution, enterococcus faecium bacterium solution and Pediococcus acidilactici bacterium solution are mixed, and mixed bacteria liquid is made；Each bacterium in the mixed bacteria liquid
The volume ratio of liquid are as follows: bacillus subtilis bacterium solution 20%-30%, bafillus natto bacterium solution 20%-30%, sticky red rhodotorula bacterium solution
15%-20%, enterococcus faecium bacterium solution 10%-15% and Pediococcus acidilactici bacterium solution 15%-25%；
Step (3): composite inorganic microelement material is added in carrier material, and fermentation substrate is made；
Step (4): in step (2) in the mixed bacteria liquid obtained and step (3) fermentation substrate obtained according to weight
Than for (0.3-0.5): 1, it is uniformly mixed, is made to fermentation material；
Step (5): it is fitted into the jumbo bag of internal setting plastic envelope bag described in will be obtained in step (4) to fermentation material, sealing
The plastic envelope bag ferments 3-5 days to get the compound organic trace element material of biosynthesis.
Preferably, the component of culture medium described in step (1) are as follows: molasses 2wt%, yeast extract 1wt%, magnesium sulfate
0.2wt%, potassium dihydrogen phosphate 0.2wt%, water 96.6wt%.
Preferably, the training of bacillus subtilis, bafillus natto, enterococcus faecium and lactic acid sheet bacterium described in step (1)
Feeding temperature is 37 DEG C, and the cultivation temperature of sticky red rhodotorula is 28 DEG C；
The bacillus subtilis, bafillus natto, enterococcus faecium, the incubation time of Pediococcus acidilactici and sticky red rhodotorula are equal
It is 14-16 hours；
The shaking speed when bacillus subtilis, bafillus natto and sticky red rhodotorula culture is 200-250r/
min；
The shaking speed when enterococcus faecium and Pediococcus acidilactici are cultivated is 20-50r/min.
Preferably, composite inorganic microelement material described in step (3) is by copper sulphate, ferrous sulfate, zinc sulfate, sulfuric acid
Manganese, calcium iodate, sodium selenite are mixed.
Preferably, carrier material described in step (3) is the mixture of corn, dregs of beans, wheat bran.
Preferably, sulfuric acid copper content is 700-1400mg/kg, ferrous sulfate content in fermentation substrate described in step (3)
For 4300-8600mg/kg, zinc sulfate content 2500-5000mg/kg, sulfuric acid manganese content is 600-1200mg/kg, calcium iodate
Content is 12-24mg/kg, and sodium selenite content is 6-13mg/kg.
Preferably, plastic envelope bag described in step (5) with a thickness of 12, the charge of each plastic envelope bag
For 600-700kg.
The application of the compound organic trace element of biosynthesis is prepared by technical solution as above, is that will prepare
Obtain the compound organic trace element material of biosynthesis be added to according to the weight adding proportion of 2.5%-5% be not added with it is micro-
Mixed feed is made in the conventional diet feed of secondary element.
The Special Significance of the microorganism and carrier material that use in the present invention is:
Bacillus subtilis 100% can reach intestine and small intestine, inhibit pathogenic bacteria, promote to grow beneficial to anaerobic bacteria, and generate lactic acid etc. to have
Machine acids reduces enteron aisle pH value, and other pathogenic bacteria is inhibited to grow indirectly, also raising immunoglobulin and antibody level, and enhancing is thin
Born of the same parents are immune and humoral immune function, raising herd immunity can synthesize alpha-amylase, protease, lipase, cellulase etc.
A variety of enzymes, enzyme system is abundant, plays a role together with the digestive enzymes in animal body (human body) in alimentary canal.
Bafillus natto can produce acid, adjust intestinal flora, and enhancing animal cell immunity, which is put, answers, and can generate a variety of eggs
White enzyme, carbohydrase, lipase, amylase, the carbohydrate of certain complexity in degrading plant feed, to improve feed
Conversion ratio.
Sticky red rhodotorula can generate α-alanine and glutamic acid, and the 1% of the very capable reachable dry weight for propylhomoserin of laying eggs, it is another
Aspect, the breeding cycle is short, speed is fast, high to absorption of trace elements rate, is converted into organic to the microelement of inorganic states
Effect is good.
Enterococcus faecium is the mushroom in animal intestinal tract normal flora, can generate lactic acid, enterococcus faecium is a kind of probiotics, right
It maintains the microbial population of animal intestinal tract ecological balance to play an important role, has in the enteron aisle health care of animal and the epidemic prevention and treatment of disease prominent
Performance out.
Pediococcus acidilactici can produce acid, be adjustable gastrointestinal bacterial flora, maintain intestinal microecology balance.In animal body to cause of disease
Microorganism has antagonistic action, inhibits pathogenic microorganism to contestable, enhances the immune function of animal body, generates beneficial generation
It thanks to product, activates the activity of acid protease, participate in the metabolism of body, prevent harmful substance from generating.
Bacillus subtilis, bafillus natto enzymatic productivity are strong, by Substrate hydrolysis at small peptide in solid ferment process
Ability is strong, can be supplied to the peptides ligand of inorganic microelement abundance.
Meanwhile the metabolite of Pediococcus acidilactici can with the activity of activator protein enzyme, strengthening solid state fermentation Substrate hydrolysis at
The conversion ratio of small peptide.
On the one hand sticky red rhodotorula can produce amino acid, provide the source of ligand, and on the other hand, itself can also be enriched with
Conversion inorganic microelement is organic trace element.
Bacillus subtilis, bafillus natto, enterococcus faecium and Pediococcus acidilactici can produce acid, and coordination can be maintained anti-
Answer required acidic environment.
Corn and wheat bran are the good carbon sources of microbial growth, while wheat bran or the good object of solid-state biofermentation
Expect loose auxiliary material, the progress of solid-state biofermentation can be effectively facilitated, dregs of beans is the good nitrogen source of microbial growth.
The carrier material selects the mixture of corn, dregs of beans, wheat bran, can be supplied to the good nutrients of microorganism
Matter and good growth physical condition, efficiently promote microbial growth.
Through studying, microelement metal ion can generate polypeptide metal element chelate object with polypeptides reactive, this kind of organic
Microelement chelate can be entered in organism by the mechanism of absorption of polypeptide, compared to inorganic salt form microelement not only
It is more easily absorbed by organism, but also some important amino acid can be provided to organism, be a kind of ideal micro-
Secondary element replenishers.In addition, polypeptide microelement chelate also has the bioactivity such as anti-oxidant, antibacterial, hypoglycemic.
The size that ligand Ao closes the coefficient of stability of metallic element is inhaled for ensuring that microelement is transported to small intestine for body
Receipts are very important.It will lead to most of substances in chelate and enteron aisle using the low ligand of the coefficient of stability to occur instead
It answers.
Compared with conventional use of amino acid ligand such as glycine, methionine and lysine, small peptide is provided relatively more
Add stable chemical bond, the coefficient of stability is higher, and chelate is more stable.
Amino acid/small peptide microelement chelate internal charge tends to be neutral, in water more difficult dissociation, chemical stability
It is good, so that metal ion avoids the ill-effect with gastric acid etc. in diet and stomach and intestine, protect the physics and chemistry of metal ion
Property, reduces the antagonism of metal ion Yu other minerals, while decreasing metal ion and cellulose, phytic acid etc.
It forms precipitating and wastes.Amino acid/small peptide microelement chelate is conducive to body fully absorbing and utilizing to metal ion,
The usage amount for saving microelement, improves the biological value etc. of microelement.
Through studying, the chelation percent of polypeptide microelement chelate obtained and bioactivity etc. under different technology conditions
There are very big differences, and especially in complexation reaction, the conditions such as material proportion, temperature, pH value are extremely important and crucial
Parameter, chelation percent and bioactivity for polypeptide microelement chelate play the role of conclusive, need according to micro
The different tests of element match reasonable process conditions and carry out complexation reaction, synthesize organic trace element.
Above-mentioned each strain is inoculated into the fermentation substrate, bacillus subtilis and natto gemma bar under aerobic conditions
Bacterium carries out breeding metabolism using the nutrition that raw material provides, and generates a variety of enzymes such as much starch enzyme, protease, cellulase, will
Starch and cellulose decomposition are at monosaccharide or polysaccharide, by breaks down proteins in raw material at polypeptide, small peptide and amino acid, these small point
Sub- a part forms covalent bond with the inorganic microelement in raw material under conditions of cofactors, forms polypeptide iron, glycine
Iron, Copper lysinate, copper methionine, zinc methionine, manganese methionine and METHIONINE AMINO-CARBOXYL IODINE COMPLEX, a part by rhodotorula glutinis, enterococcus faecium and
Pediococcus acidilactici utilizes, and makes its mass propagation, another part is attached in support material, enriches the nutritive value of raw material；It is viscous
Rhodotorula mass propagation generates mycoprotein, further enriches the nutrition of raw material, while yeast cell wall and selenium element are with covalent
Bond is closed, and yeast selenium is formed；Enterococcus faecium and Pediococcus acidilactici mass propagation secrete lactic acid and acetic acid, a part of acetic acid and cream
Acid with the situation of ionic bond in conjunction with microelement, formed copper acetate, the organic chelates such as manganese acetate, zinc lactate, ferric lactate, together
When a part of acetic acid, lactic acid be attached on raw material, improve the nutritive value of raw material again.It is distinctive by various microorganisms
Vital movement chelates inorganic microelement in various ways, can efficiently synthesize inorganic microelement organic micro
Element, while a large amount of metabolite is secreted, improve the nutritive value of raw material.
Beneficial effects of the present invention:
(1) microorganism fungus kind selected by the present invention is feed addictive license strain, does not carry any Disease-causing gene, can be high
Effect secretion biological enzyme decomposing materials, generate a large amount of peptides and amino acid substance, moreover it is possible to produce a large amount of organic acids and inhibit mould, guarantee high
While effect synthesis organic trace element, moreover it is possible to extend the shelf-life of product.
(2) organic trace element obtained by the present invention is to chelate metal ion and organic compound, forms knot
The stable chelate of structure, microelement with chelate enter corresponding target cell, participate in vital movement, reduce independent metal from
Son transports the energy of needs in animal body, improves efficiency of feed utilization.
(3) organic trace element obtained by the present invention is added in feed, be not easy in feed vitamin and enzyme etc. it is living
Property ingredient chemically reacts, and improves the vital activity of vitamin and enzyme isoreactivity substance in animal body.
(4) organic trace element obtained by the present invention can improve animal to the absorption rate of microelement, by having
Machine compound is chelated with microelement, combines microelement by phytic acid, oxalic acid etc. in animal alimentary canal, is formed not
Molten object, to make microelement smoothly reach animal intestinal mucosa and be absorbed.
(5) strain selected by the present invention can generate the flavor substance for being more conducive to fermented feed product effect, agreeable to the taste
Property is good, as sticky red rhodotorula and enterococcus faecium can improve the flavor of fermentation substrate, while by trace element chelated,
Solve the problems, such as that metal ion palatability is poor.
(6) organic trace element of the present invention is added in feed, and metal ion can be prevented to the damage of intestinal mucosa
Wound, keeps animal intestinal tract healthier.
(7) process that compound organic trace element has been prepared in the present invention, which also synchronizes, has been made biological fermentation feed, and one
It lifts two to obtain, compound organic trace element obtained is evenly distributed in fermented feed, is equivalent to and has been completed uniformly to be pre-mixed
Material, easy to use, since microelement additive amount is few, needing to be then added in feed after being diluted using carrier before use is made
With, but composite trace element produced by the present invention does not have to carry out compounding operation in advance, will can directly be uniformly distributed compound organic
Preparing complete feed in feed is added in the fermented feed of microelement.Biological fermentation feed nutritive value obtained is high, can
To promote growth of animal, animal immunizing power is improved, increases culture efficiency.
(8) mitigate environmental pollution significantly.Farm often adds the inorganic states trace element feeding of high dose in feed
Animal, it is most of to be discharged in vitro with excrement in addition to small part is absorbed by animal, natural environment is polluted, animal health and poultry are influenced
Product safety.Compound organic absorption of trace elements produced by the present invention, utilization rate are high, and biological value is also higher, in feed
Using the organic trace element of low dosage, growth result close or better with high dose inorganic microelement can get, and
The discharge amount of microelement in excrement can be made to be greatly lowered, pollution of the microelement to environment is reduced from source.
(9) method of the compound organic trace element of biosynthesis of the present invention is simple, all routines of process flow
Operation, unlike physico-chemical process need by what synthesize, substantially reduce production cost, avoid physico-chemical process synthesis
Serious pollution of the waste of generation to environment.
Specific embodiment
For a better understanding of the present invention, below with reference to the embodiment content that the present invention is furture elucidated, but it is of the invention
Content is not limited solely to the following examples.
Microorganism fungus kind of the present invention is commercialization conventional variety, can be by buying in the market, such as withered grass bud
Spore bacillus is commercially available from " Hubei Hua Da riel Science and Technology Ltd. " (trade name: only special bacterium health), and bafillus natto is commercially available
From " Chinese microorganism strain collection " (strain number BNCC184968), sticky red rhodotorula is commercially available from " China Microbiological
Culture Collection Center " (strain number BNCC220005), enterococcus faecium is commercially available from " Hubei Hua Da riel Science and Technology Ltd. "
(trade name: excellent Tetrapack), Pediococcus acidilactici are commercially available from " Chinese microorganism strain collection " (strain number
BNCC119728).It as each component of culture medium (molasses, yeast extract, magnesium sulfate) is the common raw material of feed in the present invention,
Inorganic microelement component (copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, calcium iodate, sodium selenite) that the present invention uses and
Carrier material (corn, dregs of beans, wheat bran) is common feedstuff, can be easily commercially available from market.
Embodiment 1
A kind of method of the compound organic trace element of biosynthesis, comprising the following steps:
Step (1): bacillus subtilis, bafillus natto, sticky red rhodotorula, enterococcus faecium and Pediococcus acidilactici are connect respectively
Kind is cultivated in the independent culture medium after sterilizing, the component of the culture medium are as follows: molasses 2wt%, yeast extract 1wt%, magnesium sulfate
0.2wt%, potassium dihydrogen phosphate 0.2wt%, water 96.6wt%；
The bacillus subtilis, bafillus natto, enterococcus faecium and lactic acid sheet bacterium cultivation temperature be 37 DEG C, glue red ferment
The cultivation temperature of female bacterium is 28 DEG C；
The bacillus subtilis, bafillus natto, enterococcus faecium, the incubation time of Pediococcus acidilactici and sticky red rhodotorula are equal
It is 14 hours；
The shaking speed when bacillus subtilis, bafillus natto and sticky red rhodotorula culture is 250r/min；
The shaking speed when enterococcus faecium and Pediococcus acidilactici are cultivated is 50r/min；
After cultivation, the bacterium solution of individual bacillus subtilis, the bacterium solution of bafillus natto, sticky red rhodotorula are obtained respectively
The bacterium solution of bacterium solution, the bacterium solution of enterococcus faecium and Pediococcus acidilactici；
Step (2): it by the bacterium solution for the bacillus subtilis being prepared in step (1), the bacterium solution of bafillus natto, glues
The bacterium solution of the bacterium solution of Rhodotorula sp, the bacterium solution of enterococcus faecium and Pediococcus acidilactici is mixed, and mixed bacteria liquid is made；The mixing
The volume ratio of each bacterium solution in bacterium solution are as follows: bacillus subtilis bacterium solution 30%, bafillus natto bacterium solution 30%, sticky red rhodotorula bacterium solution
15%, enterococcus faecium bacterium solution 10% and Pediococcus acidilactici bacterium solution 15%；
Step (3): composite inorganic microelement material is added in carrier material, and fermentation substrate is made；
The composite inorganic microelement material is mixed by copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, calcium iodate, sodium selenite
Conjunction is made.
The carrier material be corn, dregs of beans, wheat bran mixture, wherein corn, dregs of beans, wheat bran weight ratio be 5:
3:2。
Sulfuric acid copper content is 700mg/kg in the fermentation substrate, and ferrous sulfate content is 8600mg/kg, zinc sulfate content
For 2500mg/kg, sulfuric acid manganese content is 600mg/kg, and acid iodide calcium content is 12mg/kg, and sodium selenite content is 9mg/kg.
Step (4): in step (2) in the mixed bacteria liquid obtained and step (3) fermentation substrate obtained according to
Weight ratio is 0.3:1, is uniformly mixed, and is made to fermentation material；
Step (5): it is fitted into the jumbo bag of internal setting plastic envelope bag described in will be obtained in step (4) to fermentation material, sealing
The plastic envelope bag ferments 4 days (i.e. 96 hours) to get the compound organic trace element material of biosynthesis.
The plastic envelope bag with a thickness of 12, the charge of each plastic envelope bag is 600kg.
The application of the compound organic trace element of biosynthesis is prepared by technical solution as above, is that will prepare
The compound organic trace element material of biosynthesis described in obtaining is added to according to 5% weight adding proportion is not added with micro member
Mixed feed is made in the conventional diet feed of element.Such as: it is compound organic micro- that the biosynthesis is prepared in the present invention
Secondary element material 5kg is added in the 95kg routine diet feed for be not added with microelement and mixed feed is made.
Compound organic trace element detection: compound organic trace element obtained in step (5) is passed through into " atomic absorption light
Spectrometry " each microelement of detection chelation percent.
Testing result is shown: effectively inorganic microelement can be chelated into organic trace element using the method for the present invention,
Wherein: the chelation percent of copper is 95.08%, and the chelation percent of ferro element is 95.3%, and the chelation percent of Zn-ef ficiency is 96.11%, manganese member
The chelation percent of element is 92.33%, and the chelation percent of iodine is 93.5%, and the chelation percent of selenium element is 91.7%.
Embodiment 2
A kind of method of the compound organic trace element of biosynthesis, comprising the following steps:
Step (1): bacillus subtilis, bafillus natto, sticky red rhodotorula, enterococcus faecium and Pediococcus acidilactici are connect respectively
Kind is cultivated in the independent culture medium after sterilizing, the component of the culture medium are as follows: molasses 2wt%, yeast extract 1wt%, magnesium sulfate
0.2wt%, potassium dihydrogen phosphate 0.2wt%, water 96.6wt%；
The bacillus subtilis, bafillus natto, enterococcus faecium and lactic acid sheet bacterium cultivation temperature be 37 DEG C, glue red ferment
The cultivation temperature of female bacterium is 28 DEG C；
The bacillus subtilis, bafillus natto, enterococcus faecium, the incubation time of Pediococcus acidilactici and sticky red rhodotorula are equal
It is 16 hours；
The shaking speed when bacillus subtilis, bafillus natto and sticky red rhodotorula culture is 200r/min；
The shaking speed when enterococcus faecium and Pediococcus acidilactici are cultivated is 20r/min；
After cultivation, the bacterium solution of individual bacillus subtilis, the bacterium solution of bafillus natto, sticky red rhodotorula are obtained respectively
The bacterium solution of bacterium solution, the bacterium solution of enterococcus faecium and Pediococcus acidilactici；
Step (2): it by the bacterium solution for the bacillus subtilis being prepared in step (1), the bacterium solution of bafillus natto, glues
The bacterium solution of the bacterium solution of Rhodotorula sp, the bacterium solution of enterococcus faecium and Pediococcus acidilactici is mixed, and mixed bacteria liquid is made；The mixing
The volume ratio of each bacterium solution in bacterium solution are as follows: bacillus subtilis bacterium solution 20%, bafillus natto bacterium solution 25%, sticky red rhodotorula bacterium solution
20%, enterococcus faecium bacterium solution 15% and Pediococcus acidilactici bacterium solution 20%；
Step (3): composite inorganic microelement material is added in carrier material, and fermentation substrate is made；
The composite inorganic microelement material is mixed by copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, calcium iodate, sodium selenite
Conjunction is made.
The carrier material be corn, dregs of beans, wheat bran mixture, wherein corn, dregs of beans, wheat bran weight ratio be 4:
3:2。。
Sulfuric acid copper content is 1400mg/kg in the fermentation substrate, and ferrous sulfate content is 4300mg/kg, and zinc sulfate contains
Amount is 5000mg/kg, and sulfuric acid manganese content is 900mg/kg, and acid iodide calcium content is 18mg/kg, and sodium selenite content is 13mg/kg.
Step (4): in step (2) in the mixed bacteria liquid obtained and step (3) fermentation substrate obtained according to
Weight ratio is 0.4:1, is uniformly mixed, and is made to fermentation material；
Step (5): it is fitted into the jumbo bag of internal setting plastic envelope bag described in will be obtained in step (4) to fermentation material, sealing
The plastic envelope bag ferments 5 days (i.e. 120 hours) to get the compound organic trace element material of biosynthesis.
The plastic envelope bag with a thickness of 12, the charge of each plastic envelope bag is 650kg.
The application of the compound organic trace element of biosynthesis is prepared by technical solution as above, is that will prepare
The compound organic trace element material of biosynthesis described in obtaining is added to according to 4% weight adding proportion is not added with micro member
Mixed feed is made in the conventional diet feed of element.Such as: it is compound organic micro- that the biosynthesis is prepared in the present invention
Secondary element material 4kg is added in the 96kg routine diet feed for be not added with microelement and mixed feed is made.
Compound organic trace element detection: compound organic trace element obtained in step (4) is passed through into " atomic absorption light
Spectrometry " each microelement of detection chelation percent.
Testing result is shown: effectively inorganic microelement can be chelated into organic trace element using the method for the present invention,
Wherein: the chelation percent of copper is 94%, and the chelation percent of ferro element is 91%, and the chelation percent of Zn-ef ficiency is 92.9%, the chela of manganese element
Conjunction rate is 90.55%, and the chelation percent of iodine is 88.37%, and the chelation percent of selenium element is 90.57%.
Embodiment 3
A kind of method of the compound organic trace element of biosynthesis, comprising the following steps:
Step (1): bacillus subtilis, bafillus natto, sticky red rhodotorula, enterococcus faecium and Pediococcus acidilactici are connect respectively
Kind is cultivated in the independent culture medium after sterilizing, the component of the culture medium are as follows: molasses 2wt%, yeast extract 1wt%, magnesium sulfate
0.2wt%, potassium dihydrogen phosphate 0.2wt%, water 96.6wt%；
The bacillus subtilis, bafillus natto, enterococcus faecium and lactic acid sheet bacterium cultivation temperature be 37 DEG C, glue red ferment
The cultivation temperature of female bacterium is 28 DEG C；
The bacillus subtilis, bafillus natto, enterococcus faecium, the incubation time of Pediococcus acidilactici and sticky red rhodotorula are equal
It is 15 hours；
The shaking speed when bacillus subtilis, bafillus natto and sticky red rhodotorula culture is 230r/min；
The shaking speed when enterococcus faecium and Pediococcus acidilactici are cultivated is 35r/min；
After cultivation, the bacterium solution of individual bacillus subtilis, the bacterium solution of bafillus natto, sticky red rhodotorula are obtained respectively
The bacterium solution of bacterium solution, the bacterium solution of enterococcus faecium and Pediococcus acidilactici；
Step (2): it by the bacterium solution for the bacillus subtilis being prepared in step (1), the bacterium solution of bafillus natto, glues
The bacterium solution of the bacterium solution of Rhodotorula sp, the bacterium solution of enterococcus faecium and Pediococcus acidilactici is mixed, and mixed bacteria liquid is made；The mixing
The volume ratio of each bacterium solution in bacterium solution are as follows: bacillus subtilis bacterium solution 25%, bafillus natto bacterium solution 20%, sticky red rhodotorula bacterium solution
18%, enterococcus faecium bacterium solution 12% and Pediococcus acidilactici bacterium solution 25%；
Step (3): composite inorganic microelement material is added in carrier material, and fermentation substrate is made；
The composite inorganic microelement material is mixed by copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, calcium iodate, sodium selenite
Conjunction is made.
The carrier material be corn, dregs of beans, wheat bran mixture, wherein corn, dregs of beans, wheat bran weight ratio be 3:
3:1。
Sulfuric acid copper content is 1000mg/kg in the fermentation substrate, and ferrous sulfate content is 6400mg/kg, and zinc sulfate contains
Amount is 3600mg/kg, and sulfuric acid manganese content is 1200mg/kg, and acid iodide calcium content is 24mg/kg, and sodium selenite content is 6mg/kg.
Step (4): in step (2) in the mixed bacteria liquid obtained and step (3) fermentation substrate obtained according to
Weight ratio is 0.5:1, is uniformly mixed, and is made to fermentation material；
Step (5): it is fitted into the jumbo bag of internal setting plastic envelope bag described in will be obtained in step (4) to fermentation material, sealing
The plastic envelope bag ferments 3 days (i.e. 72 hours) to get the compound organic trace element material of biosynthesis.
The plastic envelope bag with a thickness of 12, the charge of each plastic envelope bag is 700kg.
The application of the compound organic trace element of biosynthesis is prepared by technical solution as above, is that will prepare
Obtain the compound organic trace element material of biosynthesis be added to according to 2.5% weight adding proportion be not added with it is micro
Mixed feed is made in the conventional diet feed of element.Such as: it is compound organic that the biosynthesis is prepared in the present invention
Microelement material 2.5kg is added in the 97.5kg routine diet feed for be not added with microelement and mixed feed is made.
Compound organic trace element detection: compound organic trace element obtained in step (4) is passed through into " atomic absorption light
Spectrometry " each microelement of detection chelation percent.
Testing result is shown: effectively inorganic microelement can be chelated into organic trace element using the method for the present invention,
Wherein: the chelation percent of copper is 92.1%, and the chelation percent of ferro element is 96.6%, and the chelation percent of Zn-ef ficiency is 93.75%, manganese member
The chelation percent of element is 92.75%, and the chelation percent of iodine is 91%, and the chelation percent of selenium element is 89.17%.
Application examples 1:
The mixed feed containing compound organic trace element material being prepared by the embodiment of the present invention 1 is applied to growing and fattening pigs
Raising carries out Performance of Finishing Pigs feeding experiment:
The test daily ration of test group is the cooperation containing compound organic trace element material being prepared by the embodiment of the present invention 1
Feed.
The test daily ration of control group is conventional daily ration, and wherein the content of microelement is identical as test group, but therein is added
The microelement added is inorganic microelement.
Performance of Finishing Pigs experimental result is shown in Table 1.
Table 1
| Production target | Control group | Test group | Improvement amplitude |
| Initial counterpoise/(kg/) | 40.43±1.23a | 40.17±1.27a | |
| Terminate counterpoise (kg/) | 75.20±2.14a | 77.13±1.92b | 2.57% |
| Daily gain/(g/) | 790±10.1a | 840±10.2b | 6.33% |
| Feedstuff-meat ratio | 2.78±0.04a | 2.53±0.05b | -8.99% |
| Copper content (mg/kg) in excrement | 22.3±1.21a | 16.12±1.23b | -27.71% |
| Iron content (mg/kg) in excrement | 260±5.2a | 200±5.6b | -23.08% |
| Zn content (mg/kg) in excrement | 77±5.8a | 60±5.3b | -22.08% |
| Manganese content (mg/kg) in excrement | 18.42±1.02a | 9.23±1.03b | -49.89% |
| Content of iodine (mg/kg) in excrement | 0.74±0.02a | 0.45±0.01b | -39.19% |
| The content (mg/kg) of selenium in excrement | 0.32±0.08a | 0.22±0.09b | -31.25% |
Note: (1) data are indicated with average ± SD；The identical person of same column shoulder mark lowercase indicates that difference is not significant (P > 0.05)；
Same column shoulder mark lowercase difference person indicates significant difference (P < 0.05).
(2) it feeds 44 days.
Test result is shown: growing and fattening pigs counterpoise improves 2.57% than control group, and daily gain improves 6.33% than control group,
Feedstuff-meat ratio reduces 8.99% than control group, copper in excrement, iron, zinc, manganese, iodine and selenium content reduced respectively than control group
27.71%, 23.08%, 22.08%, 49.89%, 39.19% and 31.25%.Illustrate to be compounded with by the way that addition feeding is produced by the present invention
Machine microelement material can be improved the production performance of growing and fattening pigs, improve the absorptivity of microelement, reduce the row of microelement
Put and cause the potential threat of environment.
Application examples 2:
The mixed feed containing compound organic trace element material being prepared by the embodiment of the present invention 2 is supported applied to broiler chicken
It grows, carries out meat chicken production performance feeding experiment:
The test daily ration of test group is the cooperation containing compound organic trace element material being prepared by the embodiment of the present invention 2
Feed.
The test daily ration of control group is conventional daily ration, and wherein the content of microelement is identical as test group, but therein is added
The microelement added is inorganic microelement.
Meat chicken production performance experimental result is shown in Table 2.
Table 2
| Production target | Control group | Test group | Improvement amplitude |
| Average feed intake (g/ days plumages) | 103.46±0.12a | 106.18±0.17b | 4.56% |
| Daily gain (g/ plumage) | 57.24±2.14a | 60.53±1.72b | 5.75% |
| Feedstuff-meat ratio | 1.83±0.03a | 1.74±0.02b | -4.92% |
| Death and culling rate (%) | 3.65±0.14a | 2.41±0.15b | -33.97% |
| Copper content (mg/kg) in excrement | 33.23±2.2a | 26.48±1.23b | -20.30% |
| Iron content (mg/kg) in excrement | 100.2±5.2a | 67.69±3.6b | -32.45% |
| Zn content (mg/kg) in excrement | 114.11±5.7a | 68.24±3.3b | -40.20% |
| Manganese content (mg/kg) in excrement | 22.12±1.12a | 14.06±1.13b | -36.45% |
| Content of iodine (mg/kg) in excrement | 0.82±0.02a | 0.51±0.01b | -37.65% |
| The content (mg/kg) of selenium in excrement | 0.31±0.03a | 0.19±0.02b | -40.26% |
Note: (1) data are indicated with average ± SD；The identical person of same column shoulder mark lowercase indicates that difference is not significant (P > 0.05)；
Same column shoulder mark lowercase difference person indicates significant difference (P < 0.05).
(2) feeding cycle 42 days.
Test result is shown: broiler chicken daily ingestion amount improves 4.56% than control group, and daily gain is improved than control group
5.75%, feedstuff-meat ratio reduces 4.92% than control group, and death and culling rate reduces 33.97% than control group, copper in excrement, iron, zinc, manganese,
The content of iodine and selenium reduces 20.30%, 32.45%, 40.2%, 36.45%, 37.65% and 40.26% than control group respectively.Pass through
The growth performance of broiler chicken can be promoted by feeding compound organic trace element material produced by the present invention, improve the absorption of microelement
Rate.
Application examples 3:
The mixed feed containing compound organic trace element material being prepared by the embodiment of the present invention 3 is applied to growing and fattening pigs
Cultivation carries out Performance of Finishing Pigs feeding experiment:
The test daily ration of test group is the cooperation containing compound organic trace element material being prepared by the embodiment of the present invention 3
Feed.
The test daily ration of control group is conventional daily ration, and wherein the content of microelement is identical as test group, but therein is added
The microelement added is inorganic microelement.
Performance of Finishing Pigs experimental result is shown in Table 3.
Table 3
| Production target | Control group | Test group | Improvement amplitude |
| Initial counterpoise (kg/) | 30.21±1.23a | 30.19±1.25a | |
| Terminate counterpoise (kg/) | 120.15±1.56a | 129.47±1.26b | 7.76% |
| Daily gain (g/) | 817.63±2.21a | 902.5±2.31b | 10.38% |
| Feedstuff-meat ratio | 3.01±0.02a | 2.92±0.03b | 2.99% |
| Copper content (mg/kg) in excrement | 21±1.01a | 12.4±1.03b | -40.95% |
| Iron content (mg/kg) in excrement | 85.23±5.4a | 66.54±5.5 | -21.93% |
| Zn content (mg/kg) in excrement | 58.72±5.7a | 42.33±5.3b | -27.91% |
| Manganese content (mg/kg) in excrement | 14±1.02a | 8±1.02b | -42.85% |
| Content of iodine (mg/kg) in excrement | 0.65±0.02a | 0.39±0.01b | -39.02% |
| The content (mg/kg) of selenium in excrement | 0.09±0.07a | 0.04±0.09b | -52.00% |
Note: (1) data are indicated with average ± SD；The identical person of same column shoulder mark lowercase indicates that difference is not significant (P > 0.05)；
Same column shoulder mark lowercase difference person indicates significant difference (P < 0.05).
(2) feeding cycle 110 days.
Test result is shown: growing and fattening pigs counterpoise improves 7.76% than control group, and daily gain is improved than control group
10.38%, feedstuff-meat ratio reduces 2.99% than control group, copper in excrement, iron, zinc, manganese, iodine and selenium content dropped respectively than control group
Low 40.95%, 21.93%, 27.91%, 42.85%, 39.02% and 52.00%.Illustrate produced by the present invention multiple by addition feeding
Closing organic trace element material can be improved the production performance of growing and fattening pigs, improve the absorptivity of microelement, reduce microelement
Discharge and cause the potential threat of environment.
Fattening meat quality improves test result and is shown in Table 4.
Table 4
| Meat judging quota | Control group | Test group | Improvement amplitude |
| Lean meat percentage | 74.2±7.3a | 77.9±7.2b | 4.99% |
| The thickness of backfat | 2.13±0.12a | 1.95±0.11b | -8.45% |
| Dorsal muscles cooked meat percentage | 70.2±6.9a | 73.4±6.5b | 4.56% |
| Muscle marble grain | 2.61±0.2a | 2.87±0.22b | 9.96% |
| Intramuscular fat | 3.35±0.35a | 3.87±0.36b | 15.52% |
| Meat Tenderness | 3.46±0.51a | 2.91±0.48b | -15.90% |
| Color Score | 2.64±0.14a | 2.94±0.12b | 11.36% |
| Flavour mark | 3.89±0.25a | 4.48±0.23b | 15.17% |
Note: data are indicated with average ± SD；The identical person of same column shoulder mark lowercase indicates that difference is not significant (P > 0.05)；Same column
Shoulder mark lowercase difference person indicates significant difference (P < 0.05).
Test result is shown: lean meat percentage improves 4.99% than control group, and back-fat thickness reduces 8.45% than control group, back
Flesh cooked meat percentage improves 4.56% than control group, and muscle marble grain improves 9.96% than control group, and intramuscular fat compares control group
15.52% is improved, Meat Tenderness improves 15.90%, and yellowish pink improves 11.36%, and meat flavor improves 15.17%.Explanation passes through
Addition, which feeds compound organic trace element material produced by the present invention, can improve meat quality.
Claims (8)
1. a kind of method of the compound organic trace element of biosynthesis, which comprises the following steps:
Step (1): bacillus subtilis, bafillus natto, sticky red rhodotorula, enterococcus faecium and Pediococcus acidilactici are connect respectively
Cultivated in independent culture medium kind after sterilizing, obtain respectively individual bacillus subtilis bacterium solution, bafillus natto bacterium solution,
Sticky red rhodotorula bacterium solution, enterococcus faecium bacterium solution and Pediococcus acidilactici bacterium solution；
Step (2): by the bacillus subtilis bacterium solution being prepared in step (1), bafillus natto bacterium solution, red ferment is glued
Female bacterium bacterium solution, enterococcus faecium bacterium solution and Pediococcus acidilactici bacterium solution are mixed, and mixed bacteria liquid is made；Each bacterium in the mixed bacteria liquid
The volume ratio of liquid are as follows: bacillus subtilis bacterium solution 20%-30%, bafillus natto bacterium solution 20%-30%, sticky red rhodotorula bacterium solution
15%-20%, enterococcus faecium bacterium solution 10%-15% and Pediococcus acidilactici bacterium solution 15%-25%；
Step (3): composite inorganic microelement material is added in carrier material, and fermentation substrate is made；
Step (4): in step (2) in the mixed bacteria liquid obtained and step (3) fermentation substrate obtained according to weight
Than for (0.3-0.5): 1, it is uniformly mixed, is made to fermentation material；
Step (5): it is fitted into the jumbo bag of internal setting plastic envelope bag described in will be obtained in step (4) to fermentation material, sealing
The plastic envelope bag ferments 3-5 days to get the compound organic trace element material of biosynthesis.
2. the method for the compound organic trace element of biosynthesis according to claim 1, which is characterized in that in step (1)
The component of the culture medium are as follows: molasses 2wt%, yeast extract 1wt%, magnesium sulfate 0.2wt%, potassium dihydrogen phosphate 0.2wt%, water
96.6wt%。
3. the method for the compound organic trace element of biosynthesis according to claim 1, which is characterized in that in step (1)
The bacillus subtilis, bafillus natto, enterococcus faecium and lactic acid sheet bacterium cultivation temperature be 37 DEG C, sticky red rhodotorula
Cultivation temperature be 28 DEG C；
The bacillus subtilis, bafillus natto, enterococcus faecium, the incubation time of Pediococcus acidilactici and sticky red rhodotorula are equal
It is 14-16 hours；
The shaking speed when bacillus subtilis, bafillus natto and sticky red rhodotorula culture is 200-250r/
min；
The shaking speed when enterococcus faecium and Pediococcus acidilactici are cultivated is 20-50r/min.
4. the method for the compound organic trace element of biosynthesis according to claim 1, which is characterized in that in step (3)
The composite inorganic microelement material is by copper sulphate, ferrous sulfate, zinc sulfate, manganese sulfate, calcium iodate, sodium selenite mixing system
At.
5. the method for the compound organic trace element of biosynthesis according to claim 1, which is characterized in that in step (3)
The carrier material is the mixture of corn, dregs of beans, wheat bran.
6. the method for the compound organic trace element of biosynthesis according to claim 1, which is characterized in that in step (3)
Sulfuric acid copper content is 700-1400mg/kg in the fermentation substrate, and ferrous sulfate content is 4300-8600mg/kg, and zinc sulfate contains
Amount is 2500-5000mg/kg, and sulfuric acid manganese content is 600-1200mg/kg, and acid iodide calcium content is 12-24mg/kg, sodium selenite
Content is 6-13mg/kg.
7. the method for the compound organic trace element of biosynthesis according to claim 1, which is characterized in that in step (5)
The plastic envelope bag with a thickness of 12, the charge of each plastic envelope bag is 600-700kg.
8. the application of the compound organic trace element of the described in any item biosynthesis of claim 1-7, which is characterized in that will prepare
Obtain the compound organic trace element material of biosynthesis be added to according to the weight adding proportion of 2.5%-5% be not added with it is micro-
Mixed feed is made in the conventional diet feed of secondary element.
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| CN103621979A (en) * | 2013-12-25 | 2014-03-12 | 张列飞 | Preparation and application of special compound premix for fermented feed |
| CN104798990A (en) * | 2015-05-13 | 2015-07-29 | 王升平 | Fermentation trace element premix and preparation method thereof as well as fermentation composite premix and preparation method thereof |
| CN108244369A (en) * | 2018-02-28 | 2018-07-06 | 山东和美华农牧科技股份有限公司 | A kind of lactating sow special microelement fermented feed and preparation method thereof |
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|---|---|---|---|---|
| CN103621979A (en) * | 2013-12-25 | 2014-03-12 | 张列飞 | Preparation and application of special compound premix for fermented feed |
| CN104798990A (en) * | 2015-05-13 | 2015-07-29 | 王升平 | Fermentation trace element premix and preparation method thereof as well as fermentation composite premix and preparation method thereof |
| CN108244369A (en) * | 2018-02-28 | 2018-07-06 | 山东和美华农牧科技股份有限公司 | A kind of lactating sow special microelement fermented feed and preparation method thereof |
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| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
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