CN108977360A - The method that town sewage plant tail water is used for microbial bacterial agent culture - Google Patents
The method that town sewage plant tail water is used for microbial bacterial agent culture Download PDFInfo
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- CN108977360A CN108977360A CN201811011054.2A CN201811011054A CN108977360A CN 108977360 A CN108977360 A CN 108977360A CN 201811011054 A CN201811011054 A CN 201811011054A CN 108977360 A CN108977360 A CN 108977360A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The present invention discloses a kind of method that town sewage plant tail water is used for microbial bacterial agent culture.The method includes the following steps: that (1) purifies town sewage plant tail water；(2) nutrition composition is added in the tail water of purification；(3) microbial bacteria suspension is accessed in the tail water for adding nutrition composition.The present invention cultivates microbial bacterial agent so that town sewage plant tail water is culture water source, the resource utilization to microbial inoculum production of town sewage plant tail water not only may be implemented, to obtain required microbial bacterial agent, moreover it is possible to avoid using tap water or pure water, save water resource, more economical sustainability.
Description
Technical field
The invention belongs to microbial bacterial agent preparation field, it is related to a kind of town sewage plant tail water for microbial bacterial agent culture
Method.
Background technique
Under the water body environment of pollution, due to the destruction of ecology, the original microbial flora type of water body is reduced, and leads to water
Body self-purification capacity weakens, thus more needs extraneous technology that water body is helped to restore its self-purification capacity.The method being generally available has object
Logos, chemical method, bioanalysis.Wherein, water body is administered using bioanalysis, need to be supplemented to polluted-water to its beneficial microorganism and
Its condition needed for growing, help water body solve pollution problem at this stage, are a kind of warps to achieve the purpose that administer water body
Ji effectively, the technology of green non-pollution, have good application prospect.Most of commodity microbial inoculum on the market at present such as nitrifies
Bacteria agent, denitrifying bacterium microbial inoculum, photosynthetic bacteria microbial inoculum etc. are all that culture is produced by way of fermentation.In routine
During culture production, water source used is often tap water or pure water, and water consumption is bigger, it appears uneconomical.
Water resource is just deficienter all the time in China, in addition current water pollution is relatively more, available water resource is just
Seem especially nervous, thus do not require nothing more than us and save water, also rationally to utilize existing water resource, and sufficiently excavate other
The water resource that can be utilized supplements the deficiency of existing water resource.
Summary of the invention
Based on microbial bacterial agent culture at this stage and town sewage plant tail water recycling reuse problem encountered, the present invention
Purpose be to provide a kind of method that town sewage plant tail water is used for microbial bacterial agent culture.
To achieve the above object, the method that town sewage plant tail water of the present invention is used for microbial bacterial agent culture, including it is as follows
Step:
(1) town sewage plant tail water is introduced into microbial cultivation device, and purified treatment is carried out to it；
(2) nutrition composition is added in the tail water of purification；
(3) microbial bacteria suspension is accessed in the tail water for adding nutrition composition carry out microbial inoculum culture.
Preferably, further including the steps that carrying out disinfection to town sewage plant tail water before the step 1.
Preferably, the step of described purification are as follows:
31) ammonium chloride and/or sulphite, sodium thiosulfate are added into town sewage plant tail water；
32) tail water is sufficiently stirred and is aerated, the time is 4-8 hours.
Preferably, the weight percent that the ammonium chloride adds is 0.2 ‰.
Preferably, the microbial bacterial agent type includes nitrification bacteria agent, denitrifying bacterium microbial inoculum and/or photosynthetic bacteria bacterium
Agent.
Preferably, the nutriment supplemented required for culture nitrification bacteria agent are as follows: glucose 0.1-0.5 ‰, ammonium chloride
0.1-0.5 ‰ and/or sodium bicarbonate 0.3 ‰.
Preferably, the nutriment supplemented required for culture denitrifying bacterium microbial inoculum are as follows: glucose 0.1-0.5 ‰, sodium nitrate
0.1-0.3‰。
Preferably, the nutriment supplemented required for culture photosynthetic bacteria microbial inoculum are as follows: sodium acetate 1-5 ‰, sodium bicarbonate 1-
3 ‰, ammonium chloride 0.5-1 ‰, yeast extract 0.5 ‰.
Preferably, the step of described purification are as follows:
91) tail water is continued to be heated to 35-40 DEG C, and maintains water temperature；
92) ammonium chloride and/or sulphite, sodium thiosulfate are added into the tail water of heating；
93) tail water is sufficiently stirred and is aerated, the time is 1-3 hours.The present invention is compared with other methods, beneficial to skill
Art effect has: the present invention cultivates microbial bacterial agent so that town sewage plant tail water is culture water source, and cities and towns dirt not only may be implemented
The resource utilization to microbial inoculum production of water factory's tail water, to obtain required microbial bacterial agent, moreover it is possible to avoid using tap water
Or pure water, save water resource, more economical sustainability；
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated.
Embodiment 1
The tail water for taking town sewage plant is introduced into nitrifier culture apparatus by water pump, is then carried out at purification to it
Reason, processing method are as follows: ammonium chloride is added according to 0.2 ‰ ratio, and opens stirring and aeration, the time is 8 hours.To remaining in water
After chlorine resolution completely, stop stirring, then be separately added into 0.1 ‰ glucose, 0.1 ‰ ammonium chlorides, 0.3 ‰ sodium bicarbonates.Wait cultivate
Base each component accesses nitrifier bacteria suspension after completely dissolution, in the ratio of 0.5% (v/v).Fermentation condition are as follows: aeration quantity 1L/
Min, revolving speed 80rpm, 20 DEG C of temperature.Terminate fermentation after 10d, nitrification bacteria agent product can be obtained.
Embodiment 2
Town sewage plant tail water is introduced into nitrifier culture apparatus by water pump, tail water is continued to be heated into 35-40
DEG C, and maintain water temperature；Ammonium chloride and/or sulphite, sodium thiosulfate are added into the tail water of heating；Tail water is carried out abundant
Stirring and aeration, time are 2 hours.After in water chlorine residue resolution completely after, stop stirring, then be separately added into 0.3 ‰ glucose,
0.3 ‰ ammonium chlorides, 0.3 ‰ sodium bicarbonates.After completely dissolution to culture medium each component, nitrification is accessed in the ratio of 0.5% (v/v)
Bacterium bacteria suspension.Fermentation condition are as follows: aeration quantity 1L/min, revolving speed 80rpm, 20 DEG C of temperature.Terminate fermentation after 10d, nitre can be obtained
Change bacteria agent product.
Embodiment 3
By disinfection, the tail water of purified town sewage plant, it is introduced into nitrifier culture apparatus by water pump, according to
Ammonium chloride is added in 0.2 ‰ ratio, and opens stirring；After chlorine residue resolution completely in water, stop stirring, then be separately added into
0.5 ‰ glucose, 0.5 ‰ ammonium chlorides, 0.3 ‰ sodium bicarbonates.After completely dissolution to culture medium each component, by 0.5% (v/v's)
Ratio accesses nitrifier bacteria suspension.Fermentation condition are as follows: aeration quantity 1L/min, revolving speed 80rpm, 20 DEG C of temperature.Terminate hair after 10d
Nitrification bacteria agent product can be obtained in ferment.
Embodiment 4
By the tail water of town sewage plant after disinfection, purification, it is added in denitrifying bacterium culture apparatus by water pump, according to
Ammonium chloride is added in 0.2 ‰ ratio, and opens stirring；After chlorine residue resolution completely in water, stop stirring, then be separately added into
0.1 ‰ glucose, 0.1 ‰ sodium nitrate.After completely dissolution to culture medium each component, denitrifying bacterium is accessed in the ratio of 1% (v/v)
Bacteria suspension, fermentation temperature are 20 DEG C.Terminate fermentation after 3d, denitrifying bacterium microbial inoculum product can be obtained.
Embodiment 5
By the tail water of town sewage plant after disinfection, purification, it is added in denitrifying bacterium culture apparatus by water pump, according to
Ammonium chloride is added in 0.2 ‰ ratio, and opens stirring；After chlorine residue resolution completely in water, stop stirring, then be separately added into
0.25 ‰ glucose, 0.15 ‰ sodium nitrate.After completely dissolution to culture medium each component, denitrification is accessed in the ratio of 1% (v/v)
Bacterium bacteria suspension, fermentation temperature are 20 DEG C.Terminate fermentation after 3d, denitrifying bacterium microbial inoculum product can be obtained.
Embodiment 6
By the tail water of town sewage plant after disinfection, purification, it is added in denitrifying bacterium culture apparatus by water pump, according to
Ammonium chloride is added in 0.2 ‰ ratio, and opens stirring；After chlorine residue resolution completely in water, stop stirring, then be separately added into
0.5 ‰ glucose, 0.3 ‰ sodium nitrate.After completely dissolution to culture medium each component, denitrifying bacterium is accessed in the ratio of 1% (v/v)
Bacteria suspension, fermentation temperature are 20 DEG C.Terminate fermentation after 3d, denitrifying bacterium microbial inoculum product can be obtained.
Embodiment 7
By the tail water of town sewage plant after disinfection, purification, it is added in Photosynthetic bacteria culturing device by water pump, according to
Ammonium chloride is added in 0.2 ‰ ratio, and opens stirring；After chlorine residue resolution completely in water, stop stirring, then be separately added into 1 ‰
Sodium acetate, 1 ‰ sodium bicarbonates, 0.5 ‰ ammonium chlorides, 0.5 ‰ yeast extracts.After completely dissolution to culture medium each component, by 20% (v/
V) ratio accesses photosynthetic bacteria bacteria suspension, provides light source using incandescent lamp, fermentation temperature is 20 DEG C.Terminate fermentation after 15d, i.e.,
Photosynthetic bacteria microbial inoculum product can be obtained.
Embodiment 8
By the tail water of town sewage plant after disinfection, purification, it is added in Photosynthetic bacteria culturing device by water pump, according to
Ammonium chloride is added in 0.2 ‰ ratio, and opens stirring；After chlorine residue resolution completely in water, stop stirring, then be separately added into
2.5 ‰ sodium acetates, 1.5 ‰ sodium bicarbonates, 0.75 ‰ ammonium chlorides, 0.5 ‰ yeast extracts.After completely dissolution to culture medium each component,
Photosynthetic bacteria bacteria suspension is accessed in the ratio of 20% (v/v), provides light source using incandescent lamp, fermentation temperature is 20 DEG C.It is tied after 15d
Beam fermentation, can be obtained photosynthetic bacteria microbial inoculum product.
Embodiment 9
By the tail water of town sewage plant after disinfection, purification, it is added in Photosynthetic bacteria culturing device by water pump, according to
Ammonium chloride is added in 0.2 ‰ ratio, and opens stirring；After chlorine residue resolution completely in water, stop stirring, then be separately added into 5 ‰
Sodium acetate, 3 ‰ sodium bicarbonates, 1 ‰ ammonium chlorides, 0.5 ‰ yeast extracts.After completely dissolution to culture medium each component, by 20% (v/v)
Ratio access photosynthetic bacteria bacteria suspension, using incandescent lamp provide light source, fermentation temperature be 20 DEG C.Terminate fermentation after 15d
Obtain photosynthetic bacteria microbial inoculum product.
Claims (9)
1. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, it is characterised in that: the method includes such as
Lower step:
(1) town sewage plant tail water is introduced into microbial cultivation device, and purified treatment is carried out to it；
(2) nutrition composition is added in the tail water of purification；
(3) microbial bacteria suspension is accessed in the tail water for adding nutrition composition carry out microbial inoculum culture.
2. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 1
In: further include the steps that carrying out primary filtration and disinfection to town sewage plant tail water before the step 1.
3. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 1
In: the described purification the step of are as follows:
31) ammonium chloride and/or sulphite, sodium thiosulfate are added into town sewage plant tail water；
32) tail water is sufficiently stirred and is aerated, the time is 4-8 hours.
4. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 3
In: the weight percent of the ammonium chloride addition is 0.2 ‰.
5. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 1
In: the microbial bacterial agent type includes nitrification bacteria agent, denitrifying bacterium microbial inoculum and/or photosynthetic bacteria microbial inoculum.
6. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 5
In: culture nitrification bacteria agent required for supplement nutriment are as follows: glucose 0.1-0.5 ‰, ammonium chloride 0.1-0.5 ‰ and/or
Sodium bicarbonate 0.3 ‰.
7. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 5
In: the nutriment supplemented required for culture denitrifying bacterium microbial inoculum are as follows: glucose 0.1-0.5 ‰, sodium nitrate 0.1-0.3 ‰.
8. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 5
In: the nutriment supplemented required for culture photosynthetic bacteria microbial inoculum are as follows: sodium acetate 1-5 ‰, sodium bicarbonate 1-3 ‰, ammonium chloride
0.5-1 ‰, yeast extract 0.5 ‰.
9. a kind of method that town sewage plant tail water is used for microbial bacterial agent culture, feature exist as claimed in claim 1
In: the described purification the step of are as follows:
91) tail water is continued to be heated to 35-40 DEG C, and maintains water temperature；
92) ammonium chloride and/or sulphite, sodium thiosulfate are added into the tail water of heating；
93) tail water is sufficiently stirred and is aerated, the time is 1-3 hours.
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| CN201811011054.2A CN108977360A (en) | 2018-08-31 | 2018-08-31 | The method that town sewage plant tail water is used for microbial bacterial agent culture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019867A (en) * | 2019-12-30 | 2020-04-17 | 沧州市方元生物工程有限公司 | Culture medium for cultivating bacteria for treating river channel |
Citations (2)
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|---|---|---|---|---|
| CN102730912A (en) * | 2012-07-10 | 2012-10-17 | 娄性义 | Integrated sewage-sludge treatment method |
| CN105713863A (en) * | 2016-03-30 | 2016-06-29 | 林映津 | Production technology of microbial agent through recycling of bamboo shoot processing wastewater |
-
2018
- 2018-08-31 CN CN201811011054.2A patent/CN108977360A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102730912A (en) * | 2012-07-10 | 2012-10-17 | 娄性义 | Integrated sewage-sludge treatment method |
| CN105713863A (en) * | 2016-03-30 | 2016-06-29 | 林映津 | Production technology of microbial agent through recycling of bamboo shoot processing wastewater |
Non-Patent Citations (2)
| Title |
|---|
| GUNNAR FIMLAND ET AL.: "Mutational Analysis of the Role of Tryptophan Residues in an Antimicrobial Peptide", 《BIOCHEMISTRY》 * |
| 龚雪: "污水厂尾水中微生物群落结构多样性研究", 《水污染治理技术创新与产业升级(扬州)论坛》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019867A (en) * | 2019-12-30 | 2020-04-17 | 沧州市方元生物工程有限公司 | Culture medium for cultivating bacteria for treating river channel |
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Application publication date: 20181211 |