CN108967421A - The liquid lure of fruit fly, preparation method and applications - Google Patents

The liquid lure of fruit fly, preparation method and applications Download PDF

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Publication number
CN108967421A
CN108967421A CN201811065350.0A CN201811065350A CN108967421A CN 108967421 A CN108967421 A CN 108967421A CN 201811065350 A CN201811065350 A CN 201811065350A CN 108967421 A CN108967421 A CN 108967421A
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China
Prior art keywords
fruit fly
lure
liquid
water
thickener
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CN201811065350.0A
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CN108967421B (en
Inventor
陈超
全金成
陈贵峰
江红
江一红
门友均
黄金盟
高爱华
塞斯文
齐玉洁
刘思璐
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Guangxi Academy Of Specialty Crops
Nanjing Xin'an China Green Biotechnology Co Ltd
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Guangxi Academy Of Specialty Crops
Nanjing Xin'an China Green Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention provides a kind of liquid lure of fruit fly, based on parts by weight, including following raw material: 10-60 parts of protein, 2-15 parts of thickener, 30-70 parts of water-soluble alcohol.The lure uses water-soluble alcohol rather than water is as liquid-based, and this protein fluid mixture based on water-soluble alcohol has physics and microbial stability, when water is added it can rapid retrogradation, therefore do not need using preceding " waiting overnight ".

Description

The liquid lure of fruit fly, preparation method and applications
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of liquid lure of fruit fly and preparation method thereof.
Background technique
Bactrocera trypetid is the main fruit pest in China, Southeast Asia, Australia, Hawaii, the Middle East and Africa.This It a bit include citrus fruit fly (Bactrocera dorsalis), melon and fruit fly (Bactrocera cucurbitae), Queensland trypetid (Bactrocera tryoni), citrus fruit fly (Bactrocera minax), peach fruit fly (Bactrocera zonata) etc.. The trypetid that Ceratitis spp belongs to, especially Mediterranean fruitfly (Ceratitus capitata) is south of europe, the north and the Sahara with South Africa, western australia, Mexico and the main fruit of Hawaii pest.It is North America cherry in the trypetid in Anastrepha With the pest of apple.In fruit is the primary pest of Mexico, central and south america fruit by Anastrepha trypetid.It is all this A little trypetids belong to Trypetinae.Female fruit fly in this subfamily there is very strong driving force come find protein source and Their development of ovary needs these protein sources.After having enough, they can mate and lay eggs.Male Drosophila equally can be by this The protein source of sample is attracted.
Sprayable lure based on protein has been used as prevention and treatment Bactrocera and wax in conjunction with insecticide for a long time The tool of Anastrepha.Protein source generally includes yeast autolyzate (residue during Brewage), but can also be optional Other cheap protein materials are made.These protein sprinkling lure is usually made of 50% solid for depositing in water.They Usually do not have microbial stability and the shelf-life is not grown.Male and female fruit fly all can be by such protein lure institutes Attract.Kill fruit fly effect can be reached by adding insecticide when use in lure.
Australia using the albumen bait formulation ratio of water-soluble gelling agent (such as guar gum or xanthan gum) studies have shown that do not had The albumen bait formulation for adding this kind of gelling agent is more effective.Xanthan gum and guar gum are typical dry powder thickeners, insoluble in water-soluble Property alcohol but water-soluble.
The concentration of these gelling agents and thickener usually in the flushing liquor lure of peasant's application is 0.25%-0.5%.Allusion quotation The fruit fly lure of type is the water base concentrated liquid containing about 50% solid.In use, these lures are usually diluted to The concentration of 2.5%-5.0%.Currently, on the market all fruit fly albumen lures be all it is water base, be free of any thickener, such as The orchard worker people wish to add thickener, need to propose the previous day dilution when in use and add thickener.This is because water base If fruit fly albumen lure is added to the thickener of effective concentration in advance, peasant is bought back, and can not dilute use.For agriculture For the people, water base fruit fly albumen lure on the market except validity period is short, uses if necessary in addition to unstable at present Addition thickener is also inconvenient.
But if albumen lure directly is added in thickener, it is easy agglomeration, and result in blockage at spray head.For this A way to solve the problem is usually that peasant mixes water and thickener in the previous day of application, and waits the retrogradation of mixed liquor elder generation, Then protein concentrated solution and insecticide are added in mixing viscous solution before sprinkling, this kind of lure by spraying can only be when not raining Application, because it is being easy to be washed in the rain.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of liquid lure of fruit fly, preparation method and It is applied.The lure uses water-soluble alcohol rather than water is as liquid-based, this protein fluid mixture based on water-soluble alcohol With physics and microbial stability, when water is added it can rapid retrogradation, therefore do not need using preceding " waiting overnight ".
The technical scheme is that a kind of liquid lure of fruit fly, in terms of weight fraction, including following component:
10-60 parts of protein, 2-15 parts of thickener, 30-70 parts of water-soluble alcohol.
The protein is selected from one of yeast powder, soy meal, skimmed milk power etc. or a variety of, preferred plant protein yeast Powder or soy meal, dosage are 40-60 parts preferred.
The thickener is dissolved in water but insoluble in water-soluble alcohol, if using the thickener for being dissolved in alcohol, just with background technique Aqua described in part is similar, and peasant cannot be diluted with water after buying back.
The thickener includes but is not limited to xanthan gum, guar gum, hydroxypropyl guar gum, ethyl cellulose, Methyl cellulose Element, carboxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, antler glue, starch, gelatin, pectin, alginic acid Sodium, potassium alginate, tara gum, carob.
The preparation method of fruit fly liquid lure of the present invention, in turn includes the following steps:
(1) protein and thickener are mixed evenly,
(2) it is then added in water-soluble alcohol, stirring forms the lure gel of fruit fly.
Fruit fly liquid lure of the present invention needs to be added when in use insecticide, the preferred malathion of the insecticide, Diacloden, dinotefuran, imidacloprid, thiacloprid, clothianidin, decis, cypermethrin, Cyhalothrin, kills at Acetamiprid more Rhzomorph, ethyl pleocidin, avermectin, emamectin benzoate, chlopyrifos, metrifonate etc..It is needed when fruit fly bait formulation liquid lure uses Add the aqueous solution of water or insecticide to dilute, stickiness can be increased after dilution quickly, while still can spray, the liquid of fruit fly Body lure weight concentration in insecticide aqueous solution is 2%-15%, and concentration is too low, and trapping effect is poor, and concentration is too high, and solution is too Cross it is sticky, be not easy sprinkling use.
The liquid lure of fruit fly of the present invention has the beneficial effect that:
(1) when combined with water, being formed by sprayable lure gel can be used while allocating, and generate to fruit fly very big Attraction.
(2) gained mixture gel has the advantage that feature, does not have in current commercial protein bait formulation concentrate These standby features: when storing in a sealed container, it is all stable on physics and microbiology;It can be at 5 to 10 points Sprayable gel is crowded into clock, without equal more than 24 hours.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with example, but those skilled in the art will manage Solution, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.
Embodiment 1: compare the performance of different formulations
It is used as and is lured using yeast powder, soy meal, skimmed milk power, xanthan gum, guar gum, ethyl alcohol, propylene glycol and polyethylene glycol The formula of agent concentrate is prepared according to a formula rate in table 1, forms a kind of sticky mixed liquid, and do not have Precipitating, table 1 are the viscosity of every kind of the ethyl alcohol base prepared lure concentrate of proportion, when using propylene glycol or polyethylene glycol as liquid When base, the viscosity and ethyl alcohol base phase of lure concentrate seemingly, are no longer tabulated herein.Take 10g mixed liquid that 200 millis are added It rises in water, the spraying concentration of the gel-type bait formulation that simulation peasant uses in field.Stirring mixture 5 minutes, then stands 5 points again Clock.Material is thus formed a kind of albumen bait formulation gel, property is spraying similar to common business lure.
Composition formula and performance is concentrated in 1 lure of table
Embodiment 2: compare different formulations to the attracting ability of fruit fly
Use the combination and 0.2% of citrus fruit fly (Bactrocera dorsalis), protein and thickener mixed liquor It is observed to determine which kind of combination is effectively, to be formulated as follows malathion (a kind of organophosphorus insecticide): 42 parts of protein, 8 parts of thickener, 50 parts of water-soluble alcohol, 0.2% malathion solution is added in when use, makes concentration of the lure in the solution 10%.
10 cages are taken, each cage discharges about 100 citrus fruit flies (male and female mixing) newly sprouted wings out.On peach The spray of 30cm long is chosen, every spray sprays different type bait (yeast powder/xanthan gum, the yeast powder/Guar of 5ml respectively Glue, soybean protein/xanthan gum, soybean protein/guar gum, milk powder/xanthan gum, milk powder/guar gum), and with untreated peach branch It is compared, observes the death rate after 24 hours, be shown in Table 2.
The various preparations of table 2 and combination are to the killing efficiency of citrus fruit fly
Formula Fly living Dead fly Trapping rate %
Yeast powder/xanthan gum 12 86 88%
Yeast powder/guar gum 3 100 97%
Soy meal/xanthan gum 15 89 86%
Soy meal/guar gum 4 91 96%
Milk powder/xanthan gum 39 73 65%
Milk powder/guar gum 22 74 77%
Soy meal 24 77 76%
Yeast powder 29 59 67%
Milk powder 33 50 60%
Do not handle 90 2 2%
Although soy meal, yeast powder and milk powder all can serve as the food source of citrus fruit fly, and all can serve as to lure The lure killed uses, but soy meal and yeast powder are easier to attract citrus fruit fly than milk powder.In this instance, as thickener melon Your glue shows slightly better than xanthan gum.The addition of thickener significantly improves the killing efficiency of bait formulation.
Embodiment 3: attracting ability of the more different liquid-based attractants to citrus fruit fly
We choose and prepare the preferable attractant of two trapping effects and thickening agent combination, to test different water solubilitys Influence of the alcohol to citrus fruit fly attractant effect.The group of lure becomes soy meal/yeast powder: guar gum: (ethyl alcohol gathers water-soluble alcohol Ethylene glycol, propylene glycol) it is 42%:8%:50%.The aqueous solution of malathion of the lure containing 0.2% is diluted, lure exists Weight concentration in the aqueous solution of malathion is 10%, and observation is most effective to determine which kind of combination.
10 cages are taken, each cage discharges about 100 citrus fruit flies (male and female mixing) newly sprouted wings out.On peach The spray for choosing 30cm long sprays the different lures of 5ml in every spray respectively, and with the peach spray that does not process into Row compares, and 24 as a child observed the death rate of citrus fruit fly.
The validity of the different liquid-based lures of table 3
Formula Fly living Dead fly Trapping rate %
Soy meal/guar gum/ethyl alcohol 4 91 96%
Yeast powder/guar gum/ethyl alcohol 3 100 97%
Soy meal/guar gum/polyethylene glycol 12 85 88%
Yeast powder/guar gum/polyethylene glycol 10 90 90%
Soy meal/guar gum/propylene glycol 8 95 92%
Yeast powder/guar gum/propylene glycol 7 94 93%
Do not handle 90 2 2%
Although three kinds of used prepared lures of liquid-based can have trapping effect well to citrus fruit fly in test Fruit, trapping rate is all remarkably higher than control, but the lure of ethyl alcohol base is more preferable to the attractant effect of citrus fruit fly.
Embodiment 4: various concentration dilutes comparison of the lure to the attractant effect of citrus fruit fly
An ethyl alcohol base citrus fruit fly lure formula is chosen and prepared, citrus fruit fly is lured to test concentration lure Effect.The group of lure becomes soy meal: guar gum: ethyl alcohol 42%:8%:50%.
Lure is diluted to 2%, 5%, 10% and 15% with the aqueous solution of the malathion containing 0.2%, above is dilute Releasing solution can easily spray, 5% or more dilution lure, and especially 10% and 15% lure will significantly compare water It is sticky.
10 cages are taken, each cage discharges about 100 citrus fruit flies (male and female mixing) newly sprouted wings out.On peach The spray for choosing 30cm long sprays the lure of the different diluted concentrations of 5ml in every spray respectively, and with the peach that does not process Tree spray is compared, and 24 as a child observed the death rate of citrus fruit fly.The effect of extension rate 2% is dense without other dilutions It is good to spend effect.It can be used within 5 minutes after the above lure dilution.
The validity of the different diluted concentration lures of table 4
Diluted concentration Fly living Dead fly Trapping rate %
2% 29 77 73%
5% 7 94 93%
10% 3 96 97%
15% 4 98 96%
Do not handle 88 1 1%
Embodiment 5
The lure that yeast powder/guar gum/ethyl alcohol is 42%:8%:50% is chosen, by horse traction sulphur of the lure containing 0.2% The aqueous solution of phosphorus dilutes, and weight concentration of the lure in the aqueous solution of malathion is 10%;Preparing yeast powder/water is 84%: 16% lure prepares the thickener that guar gum/water is 16%:84%, and standing etc. is to be used after mixing, and observation thickens after standing Agent reaches the time required for serviceable condition, and standing reaches after serviceable condition (time is about 12 hours), by lure with Thickener is mixed with 1: 1 weight ratio, and the aqueous solution for mixing malathion of the lure containing 0.2% is diluted, and mixing lures Weight concentration of the agent in the aqueous solution of malathion is 10%.
It is good to the time that can be used from configuration to observe and record two kinds of lure formulas.
10 cages are taken, each cage discharges about 100 citrus fruit flies (male and female mixing) newly sprouted wings out.On peach The spray for choosing 30cm long sprays the different formulations lure of 5ml in every spray respectively, and with the peach spray that does not process It is compared, 24 as a child observed the death rate of citrus fruit fly, were specifically shown in Table 5.
The different lures of table 5 reach the waiting time of sprayable state
Formula Waiting time
Yeast powder/guar gum/ethyl alcohol Add shake up after water it is immediately available, without waiting
Yeast powder/water 12 hours
By experiment as can be seen that aqueous albumen lure is using preceding needing to prepare thickener first, and configured thickening Agent, which needs to wait, can be only achieved thickening effect in 12 hours or more in order to spray, and the validity of different lures is specifically shown in Table 6.
The validity of the different lures of table 6
Formula Fly living Dead fly Trapping rate
Yeast powder/guar gum/ethyl alcohol 3 96 97%
Yeast powder/water 6 92 94%
Control 88 1 1%
As can be seen from the above table, lure formula of the invention and conventional aqueous lure formula have citrus fruit fly preferably Trapping effect, trapping rate is all remarkably higher than control, and formula trapping rate of the invention is higher.
The above content describes basic principles and main features of the invention, and the present invention is not limited to the above embodiments, Without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements are all It falls into scope of the claimed invention.

Claims (9)

1. a kind of liquid lure of fruit fly, which is characterized in that in terms of weight fraction, including following component: protein 10-60 Part, 2-15 parts of thickener, 30-70 parts of water-soluble alcohol.
2. the liquid lure of fruit fly according to claim 1, it is characterised in that the protein is selected from yeast powder, soybean One of powder, skimmed milk power are a variety of.
3. the liquid lure of fruit fly according to claim 1 or claim 2, it is characterised in that the thickener is dissolved in water but does not dissolve in Water-soluble alcohol.
4. the liquid lure of fruit fly according to claim 1, it is characterised in that the water-soluble alcohol dosage is 40-60 parts.
5. the liquid lure of fruit fly according to claim 1, it is characterised in that the thickener is selected from xanthan gum, Guar Glue, hydroxypropyl guar gum, ethyl cellulose, methylcellulose, carboxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl are fine Tie up element, antler glue, starch, gelatin, pectin, sodium alginate, potassium alginate, tara gum or carob.
6. the preparation method of -5 any fruit fly liquid lures according to claim 1, it is characterised in that successively include as follows Step:
(1) protein and thickener are mixed evenly,
(2) it is then added in water-soluble alcohol, stirring forms the dense fluids lure of fruit fly.
7. application of -5 any fruit fly liquid lures in trapping fruit fly according to claim 1.
8. application of the fruit fly liquid lure in trapping fruit fly, application method are as follows according to claim 7: using Insecticide aqueous solution diluent liquid lure, liquid lure weight concentration in insecticide aqueous solution is 2%-15%, and sprinkling uses.
9. application of the fruit fly liquid lure in trapping fruit fly according to claim 7, the insecticide is horse traction sulphur Phosphorus, Diacloden, Acetamiprid, dinotefuran, imidacloprid, thiacloprid, clothianidin, decis, cypermethrin, Cyhalothrin, Pleocidin, ethyl pleocidin, avermectin, emamectin benzoate, chlopyrifos or metrifonate.
CN201811065350.0A 2018-09-12 2018-09-12 Liquid attractant for fruit flies, preparation method and application thereof Active CN108967421B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110558342A (en) * 2019-10-18 2019-12-13 广州超威生物科技有限公司 Household fly bait composition and preparation method thereof

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Publication number Priority date Publication date Assignee Title
GB2370775A (en) * 2001-01-04 2002-07-10 Nimrod Israely Insecticidal composition based on attractant, insecticide and stabilizer, the outer surface of which expands & loses viscosity on contact with humidity
CN101015294A (en) * 2007-02-16 2007-08-15 广东省昆虫研究所 Bait glue agent for extinguishing fire ant and its prepn. method
CN101341934A (en) * 2007-07-13 2009-01-14 东莞市盛唐化工有限公司 Citrus fruit fly food attractant
CN101589728A (en) * 2009-06-30 2009-12-02 福建农林大学 Protein attractant for Bactrocera dorsalis Hendel and preparation method thereof
CN101690477A (en) * 2009-10-15 2010-04-07 杜进平 Food-sex attraction slow-release attractant for melon flies, and application thereof
CN101690478A (en) * 2009-10-15 2010-04-07 杜进平 Food-sex attraction slow-release attractant for bactrocera dorsalis, and application thereof
CN103188939A (en) * 2010-11-01 2013-07-03 农业研究组织 Novel solid bait compositions used for protecting fruits from fruit fly pests
US20130302269A1 (en) * 2012-05-14 2013-11-14 Dow Agrosciences Llc Insect attractant formulations and insect control
US20160360740A1 (en) * 2011-02-08 2016-12-15 Morton Edward Bassan, Jr. Insect Control System

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2370775A (en) * 2001-01-04 2002-07-10 Nimrod Israely Insecticidal composition based on attractant, insecticide and stabilizer, the outer surface of which expands & loses viscosity on contact with humidity
CN101015294A (en) * 2007-02-16 2007-08-15 广东省昆虫研究所 Bait glue agent for extinguishing fire ant and its prepn. method
CN101341934A (en) * 2007-07-13 2009-01-14 东莞市盛唐化工有限公司 Citrus fruit fly food attractant
CN101589728A (en) * 2009-06-30 2009-12-02 福建农林大学 Protein attractant for Bactrocera dorsalis Hendel and preparation method thereof
CN101690477A (en) * 2009-10-15 2010-04-07 杜进平 Food-sex attraction slow-release attractant for melon flies, and application thereof
CN101690478A (en) * 2009-10-15 2010-04-07 杜进平 Food-sex attraction slow-release attractant for bactrocera dorsalis, and application thereof
CN103188939A (en) * 2010-11-01 2013-07-03 农业研究组织 Novel solid bait compositions used for protecting fruits from fruit fly pests
US20160360740A1 (en) * 2011-02-08 2016-12-15 Morton Edward Bassan, Jr. Insect Control System
US20130302269A1 (en) * 2012-05-14 2013-11-14 Dow Agrosciences Llc Insect attractant formulations and insect control

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110558342A (en) * 2019-10-18 2019-12-13 广州超威生物科技有限公司 Household fly bait composition and preparation method thereof

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